A cell culture system and method

Abstract

This invention relates to the field of microbial culture, and more specifically to a cell culture system and method. Different cell mixtures are stored separately in different culture dishes. Through drive-controlled adjustment, the different culture dishes are brought together. The brought-to-be-jointed cell mixtures fuse through compression. Peristaltic stirring effectively promotes mixing while avoiding cell damage. After resetting, the cell fusion is observed, and the system continues to combine and fuse different cell types through drive control. This promotes cell fusion, reaction, and storage, facilitating the observation of experimental data and preventing contamination. Multiple culture dishes are evenly fixed on a height-adjusting actuator. Height-adjusting actuators are installed in the central support platform and two side sliding platforms. The beneficial effect of this invention is that it ensures automatic fusion and observation of cells without contamination.

Description

Technical Field

[0001] This invention relates to the field of microbial culture, and more specifically to a cell culture system and method. Background Technology

[0002] By extracting and culturing cells, and then observing their maturity in experiments, data can be easily obtained under specific conditions. Patent number CN202111343189.0 discloses a cell culture system and method. The method includes: S1: dispersing cells using proteolytic enzymes to form a cell solution; S2: applying the cell solution to a rubber membrane on a cell culture system; S3: transforming the rubber membrane into a bowl shape and adding culture medium inside the bowl-shaped membrane; S4: stretching the bowl-shaped rubber membrane using the cell culture system. The cell culture system includes a plastic frame, shallow triangular grooves, and deep triangular grooves. A shallow triangular groove is provided on each side of the plastic frame, and a deep triangular groove is provided between adjacent shallow triangular grooves. This allows for cell stretching, facilitating cell division. However, this method cannot guarantee automatic fusion and observation under contamination-free conditions. Summary of the Invention

[0003] The purpose of this invention is to provide a cell culture system and method, which has the advantage of ensuring that cells can automatically fuse and be observed in a pollution-free environment.

[0004] The objective of this invention is achieved through the following technical solution:

[0005] A cell culture system and method, the method comprising the following steps:

[0006] Step 1: Store the different cell mixtures in separate culture dishes;

[0007] Step 2: Through drive adjustment control, different culture dishes are brought together; the cell mixtures that are brought together fuse together through compression.

[0008] Step 3: Peristaltic stirring effectively promotes mixing while avoiding cell damage;

[0009] Step 4: After resetting, observe the fusion between cells, and continue to combine and fuse different cells through drive control;

[0010] Step 5: Promote cell fusion, reaction, and storage to facilitate observation of experimental data and avoid contamination.

[0011] Multiple culture dishes are provided, and the multiple culture dishes are evenly fixed on the height lifting drive. The central support platform and the two side slides are each provided with a height lifting drive. The two ends of the central support platform are respectively limited by the insertion platform to slide the two side slides. The side slides are connected and driven on the central support platform by positive and negative threaded drives.

[0012] A positive and negative threaded actuator is fixed on the central support platform. The screw shafts of the positive and negative threaded actuators are connected to the central support platform and the side slide platform through positive and negative thread engagement, respectively.

[0013] The height lifting drive includes a lifting drive, a threaded plate, a connecting longitudinal slide shaft, and a limiting fixed shaft. The drive shaft of the lifting drive rotates within the central support platform and two side slides. The drive shaft of the lifting drive is connected to the threaded plate via a threaded connection and drives the threaded plate to slide longitudinally within the limiting fixed shaft. The limiting fixed shaft is fixed within the central support platform and two side slides. The connecting longitudinal slide shaft is fixed to the threaded plate and slides longitudinally within the central support platform and two side slides.

[0014] By adding different cell mixtures to different culture dishes, and storing the entire apparatus containing the different culture dishes in a specified environment, the culture dishes with different cell mixtures are brought together by a drive to open the seals and allow the cell mixtures in the two culture dishes to fuse, according to the experimental data comparison requirements. To promote the fusion speed without damaging the cells, peristaltic stirring is used to promote fusion. The automatic drive completes the automatic driving and fusion of culture dishes with different requirements, which facilitates the observation of the reaction and avoids unnecessary contamination. Attached Figure Description

[0015] Figure 1 This is a schematic diagram of the cell culture mixing process of the present invention;

[0016] Figure 2 This is a schematic diagram of the overall structure of the present invention. Figure 1 ;

[0017] Figure 3 This is a schematic diagram of the overall structure of the present invention. Figure 2 ;

[0018] Figure 4 This is a schematic diagram of the plug-in hybrid drive structure of the present invention;

[0019] Figure 5 This is a schematic diagram of the height lifting driver of the present invention. Figure 1 ;

[0020] Figure 6 This is a schematic diagram of the height lifting driver of the present invention. Figure 2 ;

[0021] Figure 7 This is a schematic diagram of the structure of the petri dish of the present invention. Figure 1 ;

[0022] Figure 8 This is a schematic diagram of the structure of the petri dish of the present invention. Figure 2 ;

[0023] Figure 9 This is a schematic diagram of the structure of the petri dish of the present invention. Figure 3 ;

[0024] Figure 10 This is a schematic diagram of the structure of the petri dish of the present invention. Figure 4 ;

[0025] Figure 11 This is a schematic diagram of the structure of the petri dish of the present invention. Figure 5 ;

[0026] Figure 12 This is a schematic diagram of the side-concave insertion platform of the present invention.

[0027] In the diagram: 1. Central support platform; 2. Two side sliding platforms; 3. Two forward and reverse threaded actuators; 4. Height adjustment actuator; 5. Culture dish; 6. Side concave insertion frame; 7. Side concave insertion platform; 8. Lifting actuator; 9. Threaded plate; 10. Connecting longitudinal sliding shaft; 11. Limiting and fixing shaft; 12. Cell dish platform; 13. Transparent observation end cap; 14. Arc-shaped sealing plate; 15. Spring shaft; 16. Fixed top seat; 17. Top platform; 18. Elastic base; 19. Peristaltic stirring actuator; 20. Peristaltic driving boss. Detailed Implementation

[0028] The present invention will now be described in further detail with reference to the accompanying drawings.

[0029] As shown in the implementation described here,

[0030] By adding different cell mixtures to different culture dishes, and storing the entire apparatus containing the different culture dishes in a specified environment, the culture dishes with different cell mixtures are brought together by a drive to open the seals and allow the cell mixtures in the two culture dishes to fuse, according to the experimental data comparison requirements. To promote the fusion speed without damaging the cells, peristaltic stirring is used to promote fusion. The automatic drive completes the automatic driving and fusion of culture dishes with different requirements, which facilitates the observation of the reaction and avoids unnecessary contamination.

[0031] Further optimization based on the above embodiments:

[0032] Furthermore, multiple culture dishes 5 are provided, and multiple culture dishes 5 are evenly fixed on the height lifting drive 4. The height lifting drive 4 is provided in the central support platform 1 and the two side slides 2. The two ends of the central support platform 1 are respectively limited by the insertion platform to slide the two side slides 2. The side slides 2 are connected and driven on the central support platform 1 by the positive and negative thread drive 3.

[0033] This section is based on Figure 2 , Figure 3 , Figure 4 and Figure 5 The working process of the cell culture system and method example shown is as follows:

[0034] By adding different cell mixtures into different culture dishes 5, and by sealing the culture dishes 5 with different labels, observation can be carried out. The combination of the central support platform 1 and the two side sliding platforms 2 facilitates the bonding of different culture dishes 5, making fusion and observation convenient.

[0035] Further optimization based on the above embodiments:

[0036] Furthermore, a positive and negative threaded actuator 3 is fixed on the central support platform 1, and the screw shaft of the positive and negative threaded actuator 3 is connected to the central support platform 1 and the side slide platform 2 through positive and negative thread engagement.

[0037] This section is based on Figure 2 , Figure 3 , Figure 4 and Figure 5 The working process of the cell culture system and method example shown is as follows:

[0038] Driven by the positive and negative thread actuator 3, the distance between the central support stage 1 and the two side slide stages 2 is adjusted, thereby causing the culture dishes 5 with different labels to fit together, thus promoting driving and fusion observation.

[0039] Further optimization based on the above embodiments:

[0040] The height lifting drive 4 further includes a lifting drive 8, a threaded plate 9, a connecting longitudinal slide shaft 10, and a limiting and fixing shaft 11. The drive shaft of the lifting drive 8 rotates within the central support platform 1 and the two side slides 2. The drive shaft of the lifting drive 8 is connected to the threaded plate 9 via a threaded connection and drives the threaded plate 9 to slide longitudinally within the limiting and fixing shaft 11. The limiting and fixing shaft 11 is fixed within the central support platform 1 and the two side slides 2. The connecting longitudinal slide shaft 10 is fixed on the threaded plate 9 and slides longitudinally within the central support platform 1 and the two side slides 2.

[0041] This section is based on Figure 4 , Figure 5 , Figure 6 and Figure 7 The working process of the cell culture system and method example shown is as follows:

[0042] By controlling the lifting actuators 4 at different heights on the central support platform 1 and the two side slides 2, the culture dishes 5 at different heights can reach the specified height and then be fitted together; by driving the threaded engagement of the lifting actuator 8, the threaded plate 9 and the connecting longitudinal slide shaft 10 are restrained by the longitudinal sliding on the limiting fixed shaft 11, thus completing the adjustment of the height.

[0043] Further optimization based on the above embodiments:

[0044] Furthermore, the culture dish 5 includes a cell stand 12 and a transparent observation cap 13. The side end of the cell stand 12 is fixed to the connecting longitudinal sliding shaft 10, and the transparent observation cap 13 is sealed on the upper end of the cell stand 12.

[0045] This section is based on Figure 8 , Figure 9 , Figure 10 and Figure 11 The working process of the cell culture system and method example shown is as follows:

[0046] After adding different cell mixtures to the cell stand 12, the transparent observation end cap 13 is then pressed and sealed to facilitate observation and culture. Once the desired culture effect is achieved, the cells are firmly attached and fused together. The vertical sliding shaft 10 is connected to adjust the height of the cell stand 12.

[0047] Further optimization based on the above embodiments:

[0048] Furthermore, the culture dish 5 also includes an arc-shaped sealing plate 14, a spring shaft 15, a fixed top seat 16, a top platform 17, and an elastic base 18. The two ends of the cell culture dish 12 are respectively slidably sealed with arc-shaped sealing plates 14. The arc-shaped sealing plates 14 are fixed on the spring shaft 15 and are pressed and adhered to the cell culture dish 12 by the spring sealing. The spring shaft 15 is limited to slide within the fixed top seat 16, and the fixed top seat 16 is fixed within the cell culture dish 12. The outer end of the arc-shaped sealing plate 14 is fixed with the top platform 17, and the elastic base 18 is sealed and fixed at the lower end of the cell culture dish 12.

[0049] This section is based on Figure 8 , Figure 9 , Figure 10 and Figure 11 The working process of the cell culture system and method example shown is as follows:

[0050] By driving the two cell dishes 12 on the central support platform 1 and the side slide platform 2 to fit together, the two arc-shaped sealing plates 14 are simultaneously compressed by the fitting of the two top platforms 17. The compression spring shaft 15 is displaced inward within the fixed top seat 16, so that the liquid flows out through the fixed top seat 16 and mixes thoroughly with the opened cell dish 12, which facilitates the fusion and observation of cells.

[0051] Further optimization based on the above embodiments:

[0052] Furthermore, the culture dish 5 also includes a peristaltic stirring driver 19 and a plurality of peristaltic driving protrusions 20. The peristaltic stirring driver 19 is fixed to the lower end of the cell dish platform 12, and a plurality of peristaltic driving protrusions 20 are irregularly fixed on the drive shaft of the peristaltic stirring driver 19. The peristaltic driving protrusions 20 are attached to the elastic base 18.

[0053] This section is based on Figure 8 , Figure 9 , Figure 10 and Figure 11 The working process of the cell culture system and method example shown is as follows:

[0054] The peristaltic stirring actuator 19 drives multiple irregularly positioned peristaltic driving protrusions 20 to lift and lower the elastic base 18. Through the elastic reset and peristalsis of the elastic base 18, cell fusion on the cell dish 12 is promoted while avoiding damage to the cells.

[0055] Further optimization based on the above embodiments:

[0056] Furthermore, the elastic base 18 is made of rubber.

[0057] This section is based on Figure 8 , Figure 9 , Figure 10 and Figure 11 The working process of the cell culture system and method example shown is as follows:

[0058] The elastic base 18 is made of rubber, which allows for easy peristalsis and repositioning, promoting cell fusion.

[0059] Further optimization based on the above embodiments:

[0060] Furthermore, two concave insert frames 6 are fixed to both ends of the culture dish 5 on the central support platform 1; a concave insert platform 7 is fixed to the side end of the culture dish 5 on the side slide platform 2, and the concave insert platform 7 is sealed and inserted into the concave insert frame 6.

[0061] This section is based on Figure 8 , Figure 9 , Figure 10 and Figure 11The working process of the cell culture system and method example shown is as follows:

[0062] Two concave insert frames 6 are fixed to both ends of the culture dish 5 on the central support platform 1, and a concave insert platform 7 is fixed to the side end of the culture dish 5 on the side slide platform 2. During the bonding and fusion process, leakage is avoided, while communication and fusion are promoted. This facilitates the observation and recovery of fused cells for further cell experiments.

[0063] The fixed connection described in this device can refer to fixing through welding, block merging and fixing, integral casting and fixing, and threaded fixing, etc., and the appropriate method can be selected based on the installation and disassembly methods. The rotating connection can refer to fixing the bearing on the shaft by baking the bearing, and the shaft or shaft hole is provided with a spring retaining ring groove or shaft baffle. The bearing is axially fixed by the elastic retaining ring being locked in the spring retaining ring groove or shaft baffle, and rotation is achieved by the relative sliding of the bearings. The limiting sliding refers to the sliding of a slider with a ball or bearing to reduce resistance by fitting with a sliding groove, limiting the sliding trajectory, sliding direction and position, and limiting the position by limiting the position. Different connection methods are used to further distinguish the application in different usage environments.

Claims

1. A method of culturing cells, the method comprising: The method comprises the following steps: ​ Step one, store different cell mixtures into different culture dishes respectively; Step two, through driving adjustment control, the different culture dishes are adhered to each other; the adhered cell mixtures are fused through extrusion; Step three, through peristaltic stirring, the mixing is effectively promoted while the cell damage is avoided; Step four, after resetting, the fusion between the cells is observed, and through driving control, the different cells are continuously combined and fused; Step five, the fusion between the cells, the reaction and the storage are promoted, the experimental data is observed conveniently, and the pollution is avoided; The system culture dish (5) is provided with a plurality of culture dishes (5), the plurality of culture dishes (5) are uniformly fixed on the height lifting driver (4), the center support table (1) and the two side sliding tables (2) are provided with the height lifting driver (4) therein, the two ends of the center support table (1) are respectively connected to the two side sliding tables (2) through the insertion table limiting sliding, and the side sliding tables (2) are connected and driven on the center support table (1) through the forward and reverse screw drivers (3); The culture dish (5) further comprises an arc-shaped sealing plate (14), a spring shaft (15), a fixed top seat (16), a top table (17) and an elastic base (18), the two ends of the cell dish table (12) are respectively provided with the arc-shaped sealing plates (14) which are slidably and sealingly arranged, the arc-shaped sealing plates (14) are fixed on the spring shafts (15) and are extruded and adhered to the cell dish table (12) by the spring sealing, the spring shafts (15) are limitingly and slidably arranged in the fixed top seats (16), the fixed top seats (16) are fixed in the cell dish table (12), the outer ends of the arc-shaped sealing plates (14) are fixed with the top tables (17), and the elastic bases (18) are sealingly and fixedly arranged at the lower ends of the cell dish table (12); The two ends of the culture dish (5) on the center support table (1) are respectively fixed with two side concave insertion frames (6); The side end of the culture dish (5) on the side sliding table (2) is fixed with a side concave insertion table (7), and the side concave insertion table (7) is sealingly inserted into the side concave insertion frame (6).

2. A cell culture method according to claim 1, wherein, The center support table (1) is fixed with the forward and reverse screw drivers (3), and the screw shafts of the forward and reverse screw drivers (3) are respectively connected with the center support table (1) and the side sliding tables (2) through forward and reverse screw thread cooperation.

3. A cell culture method according to claim 1, wherein, The height lifting driver (4) comprises a lifting driver (8), a screw plate (9), a connecting longitudinal sliding shaft (10) and a limiting fixed shaft (11), the transmission shaft of the lifting driver (8) is rotatably arranged in the center support table (1) and the two side sliding tables (2), the transmission shaft of the lifting driver (8) is connected with the driving screw plate (9) through screw thread cooperation and is limitingly and longitudinally slidably arranged in the limiting fixed shaft (11), the limiting fixed shaft (11) is fixed in the center support table (1) and the two side sliding tables (2), the connecting longitudinal sliding shaft (10) is fixed on the screw plate (9) and is longitudinally slidably arranged in the center support table (1) and the two side sliding tables (2).

4. The cell culture method of claim 1, wherein, The culture dish (5) comprises a cell dish table (12) and a transparent observation end cover (13), the side end of the cell dish table (12) is fixed on the connecting longitudinal sliding shaft (10), and the transparent observation end cover (13) is sealingly arranged on the upper end of the cell dish table (12).

5. A cell culture method according to claim 4, wherein, The culture dish (5) further comprises a peristaltic stirring driver (19) and a plurality of peristaltic driving bosses (20), the peristaltic stirring driver (19) is fixed at the lower end of the cell dish table (12), a plurality of peristaltic driving bosses (20) are irregularly fixed on the driving shaft of the peristaltic stirring driver (19), and the peristaltic driving bosses (20) are attached to the elastic base (18).

6. The cell culture method of claim 1, wherein, The material of the elastic base (18) is rubber.

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