Device and method for observing phototaxis behavior reaction of adult weevil

Abstract

The present invention discloses a device and method for observing the phototaxis behavior reaction of adults of the family of Weevil. The device of the present invention comprises an insect activity chamber and a phototaxis chamber connected to the insect activity chamber, wherein the main body is in the shape of a cross, the center of which is the insect activity chamber, and the phototaxis chambers are symmetrically arranged around the insect activity chamber in the shape of a "cross", and each phototaxis chamber is divided into a number of phototaxis channels of the same size by a partition, and a detachable external light source and a monitoring component are provided at one end of the phototaxis channel away from the insect activity chamber, and a trapping port is provided in the phototaxis channel adjacent to the monitoring component, and a trapping bottle is connected below. When in use, the monitoring component is used to monitor and record the phototaxis behavior of adults, collect the number, sex and state of the tested adults in the insect activity chamber, each phototaxis channel and each trapping bottle, and count the phototaxis test results. The device of the present invention has a simple structure, is suitable for monitoring the phototaxis behavior reaction of adults of the family of Weevil, such as the red palm weevil, and has a fast spectral screening speed, and the screening results are accurate and reliable.

Description

Technical Field

[0001] The present invention belongs to the technical field of insect phototaxis research, and more specifically, relates to a device and method for observing the phototaxis behavior reaction of adult Curculionidae. Background Art

[0002] The red palm weevil (Rhynchophorus ferrugineus Olivier) belongs to the genus Rhynchophorus of the family Curculionidae of the order Coleoptera. It is a destructive pest of many palm plants and is now widely distributed in more than 40 countries around the world, causing serious damage. The red palm weevil is also an international quarantine pest and is included in the list of quarantine pests for imported plants, the list of quarantine pests for agriculture, and the list of quarantine pests for forestry in China.

[0003] Because the harmful characteristics of the red palm weevil are that it spends most of its time inside the tree, and only a short period of adulthood is exposed to the outside, it is extremely hidden, so it is difficult to control. At present, the control methods of the red palm weevil include chemical control, agricultural control, biological control and physical control. Among them, agricultural control only treats the symptoms and not the root cause, and the effect is very limited. Chemical control is slow to take effect, and the control effect is not ideal, and it is easy to cause pesticide residues and pollution, because the larvae of the red palm weevil are inside the tree for several months. Pheromone control has seized the weak link in the life history of the red palm weevil, that is, the short period of time when male and female adults need to find mates during the adult stage, and uses pheromones to monitor and control the red palm weevil, with obvious results. However, because the pheromone attractant core has different volatilization and release times under different climatic conditions and site conditions, the effective period is not long, and the attractant core needs to be replaced after a period of time. If it is set in a mountain forest with poor site conditions, frequent replacement of the attractant core will cause inconvenience. Using the phototaxis of insects for pest control is currently a more common physical control method. The phototaxis of insects is an important biological characteristic of insects, but due to the different light environments of insect habitats, their phototaxis also varies greatly, and their responses to light wavelengths are different. Therefore, if we want to use the phototaxis of adults of the family Curculionidae such as the red palm weevil to control them, we can only conduct experiments on the phototaxis of adult Curculionidae and observe their sensitive wavelengths to carry out targeted control, which is conducive to the application of light trapping technology in the control of adults of the family Curculionidae such as the red palm weevil.

[0004] There are many existing devices for studying the phototactic behavior of insects, but most of them have the following problems and cannot meet the phototactic behavior experiments of adults of the family Curculionidae such as the red palm weevil. First, the number of channels for observing the phototactic behavior of insects is small, which cannot meet the rapid screening of specific wavelengths within the spectral range of several hundred nanometers. Second, there is crossover of light sources, which easily affects the accuracy of the phototactic behavior experiment of insects. For example, in order to improve the screening efficiency of a large range of spectra, some devices set a large number of channels, but do not consider the problem of cross-interference of multiple light sources. Although the screening efficiency is improved, the test results lack accuracy and lose reference significance. In order to avoid the crossover of multi-channel light sources, various means such as partitioning, grading, and reflection are adopted, which are too complicated and inefficient for the screening experiment of insect phototactic behavior. Third, there is a lack of remote real-time monitoring and on-site observation functions of insect phototactic behavior, and it is impossible to accurately record data such as the time and duration of insect phototactic behavior. In summary, designing a device that can be used to observe the phototactic behavior response of adults of the family Curculionidae is conducive to the application of phototaxis in the prevention and control of adults of the family Curculionidae. Summary of the invention

[0005] The technical problem to be solved by the present invention is to overcome the defects and shortcomings of the above-mentioned existing technologies and provide a device and method for observing the phototaxis behavior reaction of adult Curculionidae.

[0006] The invention aims to provide a device for observing the phototaxis behavior reaction of adults of Curculionidae.

[0007] Another object of the present invention is to provide a method for observing the phototaxis behavior response of adult Curculionidae.

[0008] The above-mentioned purpose of the present invention is achieved through the following technical solutions:

[0009] The invention provides a device for observing phototaxis behavior reaction of adult Curculionidae. The device comprises an insect activity room and a phototaxis chamber connected to the insect activity room. The phototaxis chambers are symmetrically arranged around the insect activity room in a "cross" shape, each phototaxis chamber is divided into a plurality of phototaxis channels of the same size by a partition, the phototaxis channel and the insect activity room are separated by a detachable partition, and an opaque top cover is arranged on the top of the phototaxis chamber and the insect activity room. A sealing plate is arranged at one end of the phototaxis channel away from the insect activity room, a light inlet is arranged on the sealing plate for arranging a detachable external light source, a monitoring component is arranged on the inner side of the sealing plate, a trapping port is arranged at the bottom of the phototaxis channel adjacent to the monitoring component in the phototaxis channel, and a trapping bottle corresponding to the trapping port is arranged outside the phototaxis chamber. Except for the light inlet, the trapping port and the top cover, all of the insect activity room and the phototaxis chamber are pasted with light absorbing materials.

[0010] As a preferred embodiment, the opaque top cover on the top of the phototaxis chamber includes a transparent cover plate fixed on the top of the phototaxis chamber and a movable opaque cover plate on the transparent cover plate; the opaque top cover on the top of the insect activity chamber includes a detachable transparent cover plate and a movable opaque cover plate on the transparent cover plate. When observing phototaxis behavior, the opaque cover plate is closed to create a dark environment; after the phototaxis behavior ends, the opaque cover plate can be opened to observe the activity of the insects in the phototaxis chamber through the transparent cover plate.

[0011] As an optional embodiment, the main body of the device for observing the phototaxis behavior reaction of adult Curculionidae of the present invention is a container composed of a cross-shaped bottom plate, side plates, a sealing plate and a transparent cover plate of the same size as the bottom plate; the sealing plates are located at the four ends of the cross-shaped bottom plate, and the remaining positions are side plates; the main body of the device is divided into 5 areas of equal size, the center is an insect activity room, and the phototaxis rooms are symmetrically arranged around the insect activity room in a "cross" shape; the phototaxis room is divided into a number of phototaxis channels of equal size by partitions parallel to the side plates, The phototaxis channel and the insect activity room are separated by a detachable partition; a sealing plate is provided at one end of the phototaxis channel away from the insect activity room, a light inlet is provided on the sealing plate, and a detachable external light source is set, a monitoring component is provided on the inner side of the sealing plate, a trapping port is provided at the bottom of the phototaxis channel adjacent to the monitoring component in the phototaxis channel, and a trapping bottle corresponding to the trapping port is provided outside the phototaxis room; the insect activity room and the phototaxis room are all pasted with light-absorbing materials except the light inlet, the trapping port and the top cover; a detachable opaque cover is also provided on the top of the insect activity room and each phototaxis room.

[0012] Specifically, the central area of ​​the transparent cover is detachable, that is, the transparent cover on the top of the insect activity room is detachable, which is convenient for placing the test insect source into the device of the present invention.

[0013] As an optional embodiment, the present invention further provides a detachable insect placement chamber composed of detachable partitions, so as to facilitate the placement of the test insect source into the device of the present invention through the insect placement chamber.

[0014] Specifically, the insect placement chamber is made of an opaque material, or light-absorbing materials are pasted around the insect placement chamber, so that the test insect source can first adapt to the dark environment, which is beneficial to the phototaxis experiment.

[0015] Specifically, the insect placing room is 60 cm long, 60 cm wide and 20 cm high.

[0016] The present invention adheres black light-absorbing velvet paper to the left and right sides and the bottom of all the channels except the light inlet, the trapping port and the top cover, thereby ensuring that the light source emitted by the light source is only visible at the light inlet, so that when the adults of the Curculionidae family choose light sources of different wavelengths, they are only attracted or avoided by the light source at the light inlet and captured by the trapping bottle, thereby accurately screening the attraction and avoidance effects of light sources of different wavelengths on the adults of the red palm weevil, and effectively avoiding the influence of other factors on the experimental results.

[0017] Specifically, the light-absorbing material is black velvet paper.

[0018] Specifically, the detachable external light source is in the shape of a cuboid, which internally includes a filter, a group of LED lamp beads with a specific wavelength, a power supply and a fan control group, and externally includes voltage and current setting knobs, a liquid crystal display, a fan port and a power cord.

[0019] Specifically, the monitoring component is arranged at the corner between the bottom plate and the sealing plate.

[0020] Specifically, the monitoring component is a miniature real-time monitoring camera.

[0021] Specifically, the caliber of the trapping bottle is the same as that of the trapping port, and a funnel is provided between the trapping port and the trapping bottle.

[0022] Specifically, each phototaxis chamber contains 3 groups of phototaxis channels of equal width, and the entire device has a total of 12 groups of phototaxis channels.

[0023] The present invention adopts a 4-way 12-channel design, which overcomes the problem that the existing insect phototaxis behavior screening device has a small number of channels and cannot meet the rapid screening of specific wavelengths within a spectral range of several hundred nanometers.

[0024] Specifically, the length of the phototaxis channel is 80 cm, the width is 25 cm, and the height is 25 cm. Due to the small space of the existing device, the channel is not long enough, which is not suitable for the long-distance phototaxis of large coleopteran flying beetles such as red palm weevils. In addition, the channel size is too small, which will cause large coleopteran insects to collide and change direction multiple times when flying towards the light source or double the phototaxis behavior selection time, prolonging the experimental time or even changing the experimental results, causing data errors.

[0025] Specifically, the light inlet has a diameter of 6.5 cm, is 9.25 cm away from the left and right partitions respectively, is 8.5 cm away from the bottom plate, and is 10 cm away from the upper cover plate.

[0026] Specifically, the diameter of the trapping bottle is 10 cm, and it is 7.5 cm away from the two side partitions and 5 cm away from the sealing plate.

[0027] The present invention also provides a method for observing the phototactic behavior reaction of adult Curculionidae insects by using the device, the method comprising: arranging light sources at intervals at the light inlet position and numbering the phototactic channels where the light sources are located, placing the test insect source in the insect activity room, removing the detachable partition, covering the cover, opening the monitoring component to monitor and record the phototactic behavior of the adult insects, and after the test, opening the cover, collecting and counting the number, gender and state of the test adult insects in the insect activity room, each phototactic channel and each trapping bottle.

[0028] Specifically, the number of insect sources for testing is not less than 160, and the ratio of female to male adults is 1:1.

[0029] Specifically, the number of insect sources for testing was 200, including 100 female and 100 male adults.

[0030] Specifically, the insect source for testing is an adult red palm weevil.

[0031] The present invention has the following beneficial effects:

[0032] 1. The device of the present invention not only satisfies the rapid screening of insects' phototaxis responses to different wavelengths within a wide spectral range, thus shortening the screening time; at the same time, it effectively avoids the interference of light sources' intersection and crossover in the simultaneous screening of multiple light sources on the phototaxis behavior of insects, thus ensuring that the screening test results are more accurate and effective.

[0033] 2. The spatial design of the device described in the present invention is suitable for the habit of large coleopteran beetles such as red palm weevils to fly long distances to light, so that the insects' choice of wavelength is closer to the selection behavior in the real environment, and avoids the situation where the large coleopteran insects collide with and change direction multiple times when flying toward the light source due to the channel size being too small, or the phototaxis behavior selection time is doubled, which prolongs the experimental time and even changes the experimental results, causing data errors.

[0034] 3. Since the insect phototaxis experiment is generally conducted in a closed dark space, in order to develop the subsequent multifunctional spectrum insect trap, in addition to obtaining the data of the number of insects collected in the trapping barrel, it is also necessary to monitor the various selection behaviors of insects in the process of selecting light sources of different wavelengths, and monitor the time when insects select different light sources. This requires each experiment to last all night, and manual monitoring cannot be achieved, or manual observation will frequently interfere with the experimental process. The device of the present invention installs a real-time monitoring device inside each independent channel, making the experimental results more real and objective, and making the collected data types richer, which can meet the experimental data requirements required for screening tests and subsequent R&D products.

[0035] 4. Except for the light inlet, the trapping port and the top cover, the left and right sides and the bottom of all other channels and the central insect chamber of the device described in the present invention are all pasted with black light-absorbing velvet paper to ensure that the light emitted by the light source is only visible at the light inlet. When the adult red palm weevil chooses light sources of different wavelengths, it is only attracted or avoided by the light source at the light inlet and captured by the trapping bottle. The attraction and avoidance effects of light sources of different wavelengths on the adult red palm weevil are accurately screened, effectively avoiding the influence of other factors on the experimental results. BRIEF DESCRIPTION OF THE DRAWINGS

[0036] Figure 1 It is a three-dimensional diagram of the device described in the present invention.

[0037] Figure 2 It is a top view of the device described in the present invention.

[0038] Figure 3 Schematic diagram of the phototaxis chamber of the device described in the present invention.

[0039] Figure 4 Schematic diagram of the setup of a detachable light source for phototaxis monitoring experiments.

[0040] Figure 5 The statistical results of the differences in the tropism of red palm weevils with different wavelengths. In the figure, capital letters AB represent extremely significant differences, P < 0.01; lowercase letters ab represent significant differences, P < 0.05.

[0041] Figure captions: 1-insect activity chamber; 2-phototaxis chamber; 3-cross-shaped bottom plate; 4-side plate; 5-partition; 6-detachable external light source; 7-trapping port; 8-monitoring component; 9-sealing plate; 10-light inlet; 11-trapping bottle; 12-funnel; 13-transparent cover; 14-opaque cover, 15-insect chamber; 21-phototaxis channel. DETAILED DESCRIPTION

[0042] The present invention is further described below in conjunction with the accompanying drawings and specific examples, but the examples do not limit the present invention in any form. Unless otherwise specified, the reagents, methods and equipment used in the present invention are conventional reagents, methods and equipment in the art.

[0043] Unless otherwise specified, the reagents and materials used in the following examples are commercially available.

[0044] Embodiment 1 Device for observing the phototaxis behavior of adult Curculionidae

[0045] The present invention provides a device for observing the phototaxis behavior of adult Curculionidae, the stereogram of the device is as follows: Figure 1 As shown, its top view is as Figure 2 As shown, the schematic diagram of the phototaxis chamber in the device is as shown Figure 3 As shown. Figures 1 to 3As shown, the device for observing the phototaxis behavior reaction of adult Curculionidae of the present invention comprises an insect activity room 1 and a phototaxis room 2 connected to the insect activity room 1; the phototaxis room 2 is symmetrically arranged around the insect activity room 1 in a "cross" shape, each phototaxis room is divided into a plurality of phototaxis channels 21 of the same size by a partition 5, the phototaxis channel 21 is separated from the insect activity room 1 by a detachable partition, and an opaque top cover is arranged on the top of the phototaxis room 2 and the insect activity room 1; a sealing plate 9 is arranged at one end of the phototaxis channel 21 away from the insect activity room 1, a light inlet 10 is arranged on the sealing plate 9, and a detachable external light source 6 is set, a monitoring component 8 is arranged on the inner side of the sealing plate 9, a trapping port 7 is arranged at the bottom of the phototaxis channel adjacent to the monitoring component in the phototaxis channel, and a trapping bottle 11 corresponding to the trapping port 7 is arranged outside the phototaxis room; except for the light inlet, the trapping port and the top cover, all the insect activity room 1 and the phototaxis room 2 are pasted with light absorbing materials.

[0046] The opaque top cover in the above device includes a transparent cover plate 13 fixed on the top of the phototaxis chamber and a movable opaque cover plate 14 on the transparent cover plate 13. When observing the phototaxis behavior, the opaque cover plate 14 is closed to create a dark environment; after the phototaxis behavior ends, the opaque cover plate 14 can be opened to observe the activity of the insects in the phototaxis chamber through the transparent cover plate 13.

[0047] Specifically, the main body of the device for observing the phototaxis behavior reaction of adult Curculionidae of the present invention is a container composed of a cross-shaped bottom plate 3, a side plate 4, a sealing plate 9 and a transparent cover plate 13 of the same size as the bottom plate; the sealing plate 9 is located at the four ends of the cross-shaped bottom plate 3, and the remaining positions are side plates 4; the main body of the device is divided into 5 areas of equal size, the center is an insect activity chamber 1, and the rest are phototaxis chambers 2; the phototaxis chamber 2 is divided into 3 phototaxis channels 21 of equal size by two partitions 5 parallel to the side plates 4, and the phototaxis channel 21 is 80 cm long, 25 cm wide, and 25 cm high; the phototaxis channel 21 is separated from the insect activity chamber 1 by a detachable partition; a sealing plate 9 is provided at the end of the phototaxis channel 21 away from the insect activity chamber 1, and a light inlet 10 is provided on the sealing plate 9, the diameter of the light inlet 10 is 6.5 cm, and the distance from the left and right side partitions is 9.25 cm respectively, and the distance from the bottom plate is 8.5 cm m, 10cm away from the upper cover, can be used to set a detachable external light source 6, the detachable external light source 6 is a rectangular parallelepiped, its interior includes a filter, a LED lamp bead group of a specific wavelength, a power supply and a fan control group, and the exterior includes a voltage and current setting knob, a liquid crystal display screen, a fan port and a power cord; a monitoring component (a miniature real-time monitoring camera) 8 is provided at the corner of the bottom plate 3 and the sealing plate 9; a trapping port 7 is provided at the bottom of the phototaxis channel 21 adjacent to the monitoring component 8 in the phototaxis channel 21, and a trapping bottle 11 corresponding to the trapping port 7 is provided outside the phototaxis chamber 2, the diameter of the trapping bottle is 10cm, and it is 7.5cm away from the two side partitions and 5cm away from the sealing plate; except for the light inlet, the trapping port and the top cover, all the insect activity room 1 and the phototaxis chamber 2 are pasted with light-absorbing materials to avoid the cross influence of light; the insect activity room 1 and each phototaxis chamber 2 are also provided with a detachable opaque cover on the top.

[0048] The central area of ​​the transparent cover plate 13 described in the above device is detachable, that is, the transparent cover plate 13 on the top of the insect activity room 1 is detachable, which is convenient for placing the test insect source into the device described in the present invention.

[0049] The present invention also provides a detachable insect placement chamber 15 composed of detachable partitions, which is convenient for placing the test insect source into the device of the present invention through the insect placement chamber 15. The insect placement chamber is made of opaque material, or light-absorbing material is pasted around the insect placement chamber, so that the test insect source can first adapt to the dark environment, which is beneficial to the phototaxis experiment; the insect placement chamber is 60 cm long, 60 cm wide and 20 cm high.

[0050] Example 2 Observation of phototactic behavior of red palm weevil

[0051] The present invention takes the red palm weevil as an example to illustrate the use method of the device for observing the phototaxis behavior response of adult Weevilidae described in Example 1 of the present invention.

[0052] The specific steps are as follows:

[0053] (1) 100 female and male red palm weevils of laboratory standard insect sources were raised under the conditions of constant temperature of 28°C, humidity of 65%, and light cycle of L:D = 14h:10h (light from 5:00 am to 19:00 pm, darkness for the rest of the time). Existing studies have shown that the age of insects has little effect on the spectral selection range of insects. Therefore, the experiment selected insects from the initial stage of eclosion to the active stage for 30 to 45 days or when the total number of test insects was less than 80, and then replaced with new eclosion insect sources.

[0054] (2) Select the phototaxis channel for the experiment, set up a detachable light source and number the phototaxis channel for the experiment. The trapping bottles are also numbered according to the corresponding channels. The schematic diagram of the setting of the detachable light source in the phototaxis behavior reaction monitoring experiment is shown in the figure. Figure 4 As shown; a Jooan JA-A1-wifi version micro mobile phone remote camera was selected as a micro real-time monitoring camera (length, width and height of 3.4 cm each), and it was placed in the middle of the angle between the light inlet corresponding to the test channel and the center line of the trapping bottle mouth;

[0055] (3) The experimental wavelength range of this embodiment is 265nm~795nm, including ultraviolet, visible light and near-infrared spectra, and is divided into 10 groups of experiments, with 6 wavelengths in each group in the visible light spectrum range and 3 wavelengths in each group in the ultraviolet and infrared spectrum ranges, see Table 1 for details.

[0056] (4) The six wavelengths of the test are distributed in sequence at the light sources of each channel (for example Figure 4 ), the same channel position of each wavelength group was repeated 3 times, and the 4 directions were rotated once each, and each wavelength group was tested 12 times in total.

[0057] (5) The preparatory work before the experiment started at 18:30 every day: prepare the test light source, determine the position and place the light source, turn on the light source, set the current to 250mA and the voltage to 12V; place the prepared test insect source in a detachable insect chamber, and place the insect chamber in the insect activity chamber. At 19:00, remove the isolation board of the insect chamber, cover the transparent cover of the central area (insect activity chamber), and then cover the opaque covers of each channel (phototropism chamber) and the central area (insect activity chamber); open the Joan Smart Link app on the mobile phone, and use the camera to monitor the behavior and time of the adult insects in each recording channel approaching, selecting the light source, or returning to the exit channel in real time. The camera is also equipped with a memory card, and the monitoring video records can be viewed at any time; if special circumstances are encountered during monitoring, the opaque cover of the corresponding part can be opened to observe and inspect through the transparent cover, which will not affect the progress of the experiment.

[0058] (6) The experiment started at the same time every day, with one group per day; the experimental time was from 18:30 to 8:00 the next day. At 8:00 the next day, the two covers were opened and the number, sex, and state of adult red palm weevils in the central insect room, each channel, and each trapping bottle were collected and counted.

[0059] (7) After the experiment is completed, the test insects are separated into male and female types and placed in a breathable plastic box with a length of 25 cm, a width of 15 cm, and a height of 15 cm for breeding. They are supplemented with 10% honey cotton balls for nutrition. A small vacuum cleaner is used to clean all channels of the device, and a fan is used to ventilate and disperse the odor until the next experiment.

[0060] Through the above experiments, the phototaxis behavior of large flying beetles such as the red palm weevil can be observed dynamically in real time, and their sensitive wavelengths, duration of action and duration can be quickly screened, providing a technical basis for the next step of developing multi-period programmed multi-spectrum insect attractant lamps specifically for red palm weevils.

[0061] Table 1 Wavelength grouping

[0062]

[0063]

[0064] Example 3 Analysis of observation results of phototactic behavior of red palm weevils

[0065] The present invention uses the method described in Example 2 to conduct multiple groups of experiments and repeat them, and the statistical results are shown in Table 2:

[0066] Table 2

[0067]

[0068]

[0069] After SPSS variance analysis, it can be seen that the total number of red palm weevils trapped under different wavelengths of light and the number of male and female weevils trapped under different wavelengths are significantly different (P<0.01). The data results are shown in Table 3:

[0070] Table 3 Results of variance analysis

[0071]

[0072]

[0073] Because the sample sizes of each wavelength group are different, the present invention also uses the Scheffe method to perform multiple comparisons. The statistical results of the differences in the tropism of red palm weevils at different wavelengths are as follows: Figure 5 As shown in the figure, capital letters AB represent extremely significant differences, P < 0.01; lowercase letters ab represent significant differences, P < 0.05. Figure 5It can be seen that the difference in the tropism of the red palm weevil in each group of wavelength comparison is significant (different letters indicate significant differences, P < 0.05), and the device can also detect significant and extremely significant differences in the wavelength gradient range of 5 to 10 nm. This result shows that the device for observing the phototactic behavior response of adult Curculionidae of the present invention can meet the phototactic behavior experiments of adults of Curculionidae such as the red palm weevil, is suitable for monitoring the phototactic behavior response of adults of Curculionidae such as the red palm weevil, and the speed of spectral screening is fast, and the screening results are accurate and reliable.

[0074] The above embodiments are preferred implementation modes of the present invention, but the implementation modes of the present invention are not limited to the above embodiments. Any other changes, modifications, substitutions, combinations, and simplifications that do not deviate from the spirit and principles of the present invention should be equivalent replacement methods and are included in the protection scope of the present invention.

Claims

1. A device for observing the phototaxis behavior of adult Curculionidae, characterized in that: It comprises an insect activity room (1) and a phototropism room (2) connected to the insect activity room (1); The phototaxis chambers (2) are symmetrically arranged in a "cross" shape around the insect activity chamber (1), each phototaxis chamber is divided into a plurality of phototaxis channels (21) of the same size by a partition (5), the phototaxis channel (21) and the insect activity chamber (1) are separated by a detachable partition, and an opaque top cover is provided on the top of the phototaxis chamber (2) and the insect activity chamber (1); a sealing plate (9) is provided at one end of the phototaxis channel (21) away from the insect activity chamber (1), and a light inlet (10) is provided on the sealing plate (9) for arranging a detachable external light source (6) A monitoring component (8) is provided on the inner side of the sealing plate (9); a trapping port (7) is provided at the bottom of the phototaxis channel adjacent to the monitoring component (8) in the phototaxis channel (21); a trapping bottle (11) corresponding to the trapping port (7) is provided on the outside of the phototaxis chamber (2); light absorbing materials are pasted on all of the insect activity chamber (1) and the phototaxis chamber (2) except for the light inlet, the trapping port and the top cover; the opaque top cover on the phototaxis chamber (2) includes a transparent cover plate (13) fixed on the top of the phototaxis chamber and a movable light-proof cover plate (14) on the transparent cover plate; The main body of the device is a container composed of a cross-shaped bottom plate (3), side plates (4), a sealing plate (9) and a transparent cover plate (13) of the same size as the bottom plate; the sealing plate (9) is located at the four ends of the cross-shaped bottom plate (3), and the remaining positions are side plates (4); the main body of the device is divided into five areas of equal size, the center of which is an insect activity chamber (1), and the phototaxis chamber (2) is symmetrically arranged around the insect activity chamber (1) in a "cross" shape; the phototaxis chamber (2) is divided into a plurality of phototaxis channels (21) of the same size by a partition (5) parallel to the side plate (4), and the phototaxis channel (21) is separated from the insect activity chamber (1) by a detachable partition; A sealing plate (9) is provided at one end of the light channel (21) away from the insect activity chamber (1), and a light inlet (10) is provided on the sealing plate (9) for arranging a detachable external light source (6). A monitoring component (8) is provided on the inner side of the sealing plate (9). A trapping port (7) is provided at the bottom of the phototaxis channel adjacent to the monitoring component (8) in the phototaxis channel (21), and a trapping bottle (11) corresponding to the trapping port (7) is provided outside the phototaxis chamber (2); except for the light inlet, the trapping port and the top cover, all of the insect activity chamber (1) and the phototaxis chamber (2) are pasted with light absorbing materials; and a detachable light-proof cover plate (14) is also provided on the top of the insect activity chamber (1) and each phototaxis chamber (2).

2. The device according to claim 1, characterized in that: The transparent cover plate (13) on the top of the insect activity room (1) is detachable.

3. The device according to claim 1 or 2, characterized in that: The detachable external light source (6) includes a filter, a group of LED lamp beads with a specific wavelength, a power supply and a fan control group, and includes voltage and current setting knobs, a liquid crystal display, a fan port and a power cord.

4. The device according to claim 1 or 2, characterized in that: The monitoring component (8) is arranged at the corner between the bottom plate (3) and the sealing plate (9).

5. The device according to claim 1 or 2, characterized in that: The caliber of the trapping bottle (11) is the same as that of the trapping port (7), and a funnel (12) is provided between the trapping port (7) and the trapping bottle (11).

6. The device according to claim 1 or 2, characterized in that: The light absorbing material is black velvet paper.

7. A method for observing the phototaxis behavior of adult Curculionidae insects using the device according to any one of claims 1 to 6, characterized in that: Light sources are arranged at intervals at the light inlet and numbered, and the test insect source is placed in the insect activity room (1), the detachable partition is removed, the cover is closed, and the monitoring component is opened to monitor and record the phototaxis behavior of the adult insects. After the test, the cover is opened, and the number, sex and state of the test adult insects in the insect activity room, each phototaxis channel, and each trapping bottle are collected and counted.

8. The method according to claim 7, characterized in that: The number of insect sources for testing shall not be less than 80.

Images