Sensor for measuring viscosity and concentration of glucose solution based on photoelectric response and biosensing technology

Abstract

The present application provides a sensor for measuring the viscosity and concentration of glucose solution based on photoelectric response and biosensing technology. Specifically, the present application provides a sensor for measuring the viscosity and concentration of glucose solution, comprising a light source module, a reaction module, a glucose viscosity response module, a glucose concentration response module and a response measurement and analysis module. The sensor of the present application can complete the measurement of the viscosity and concentration of glucose solution in the same system, i.e. first measuring the glucose viscosity and then measuring the glucose concentration. The traditional measurement of glucose concentration and viscosity needs to be completed separately in different systems. The present application can reduce the measurement steps, reduce the amount of sample used, and achieve fast response speed, stable detection results, reliability and high efficiency.

Description

Technical Field

[0001] This invention relates to the field of medical sensor technology, and more specifically to a sensor based on photoelectric response and biosensing technology that can measure the viscosity and concentration of glucose solutions. Background Technology

[0002] Viscosity is a physicochemical property of a substance, a measure of the viscosity of a fluid, and an expression of the fluid's flow dynamics and internal friction. It has important applications in many fields. In clinical testing, the measured viscosity of bodily fluids such as sweat and serum can reflect physiological conditions and is an important diagnostic criterion. For example, in cardiovascular diseases such as myocardial infarction and cerebral infarction, it is associated with abnormalities in many blood parameters. Measuring serum viscosity is a very simple and effective method. Elevated serum viscosity indicates that the blood may contain more blood glucose and blood lipids. Increased blood viscosity significantly increases blood flow resistance, thus affecting normal microcirculation perfusion. In addition, viscosity is also an important parameter for some liquid pharmaceuticals, reflecting the concentration and quality of the drug.

[0003] Viscosity measurement currently mainly includes methods such as rotation, capillary action, and vibration. However, current viscosity measurement methods generally cannot achieve simple measurements, requiring moving parts and failing to meet specific requirements regarding applicability, measurement accuracy, response speed, economy, and operational difficulty. For human body fluids, traditional viscosity testing instruments are relatively large, measure large volumes, are complex to operate, require highly skilled operators, are expensive, and cannot be used only once, making clinical application difficult. Current medical sensors primarily focus on detecting the concentration of relevant substances in body fluids; sensors for measuring body fluid viscosity are relatively few, and it is difficult to simultaneously measure the viscosity and concentration of a solution using a small amount of body fluid.

[0004] Biosensors are devices that use immobilized biological materials as molecular recognition elements to selectively identify specific analytes. They are devices that are sensitive to the analyte and convert its concentration into an electrical signal for detection. By designing a suitable biosensor structure, it is possible to selectively identify glucose molecules and measure their concentration. Currently, existing sensors struggle to simultaneously measure both the viscosity and concentration parameters of glucose within the same detection system.

[0005] Therefore, there is an urgent need in this field to develop sensors that can measure viscosity parameters and concentration in the same system of glucose solution. Summary of the Invention

[0006] The purpose of this invention is to provide a sensor that can detect both viscosity and concentration parameters in the same glucose solution system.

[0007] In a first aspect, the present invention provides a sensor for measuring the viscosity and concentration of a glucose solution, comprising: a light source module, a reaction module, a glucose viscosity response module, a glucose concentration response module, and a response measurement and analysis module, wherein...

[0008] The light source module includes an excitation light source, an optical fiber, an optical lens, a shutter, and a timer; the reaction module includes a substrate and a bottomless container.

[0009] The glucose viscosity response module includes a first electrode, a second electrode, and a sensitive membrane element; the glucose concentration response module includes a third electrode, a fourth electrode, and a reference electrode.

[0010] The response measurement and analysis module includes a measurement interface, a galvanometer, and equipment for data recording and analysis.

[0011] Furthermore, the first electrode, second electrode, third electrode, fourth electrode, and reference electrode are arranged in parallel in the reaction module, the sensitive membrane element is located on the first electrode, the light source module is located directly above the first electrode, and the response measurement and analysis module is connected to the glucose viscosity response module and the glucose concentration response module, respectively.

[0012] In another preferred embodiment, the glucose viscosity response module and the glucose concentration response module are not connected to each other.

[0013] In another preferred embodiment, the sensitive membrane element is an organic semiconductor material that has good stability to water and is fixed to the first electrode by one or more methods selected from the group consisting of: solution drop coating, physical adsorption, chemical fixation, etc.

[0014] In another preferred embodiment, the light source module excites a light source to emit light of a specific wavelength, the wavelength of which is the absorption wavelength of the sensitive membrane element.

[0015] In another preferred embodiment, the substrate comprises a substrate made of one or more materials selected from the group consisting of silicon dioxide, PET, or other organic or inorganic materials that are chemically inert, do not affect electrode response, and have good insulation properties.

[0016] In another preferred embodiment, the first electrode comprises an electrode made of one or more materials selected from the group consisting of gold, or other materials that do not affect photoelectric response, are chemically stable, and have good electrical conductivity.

[0017] In another preferred embodiment, the second electrode comprises an electrode made of one or more materials selected from the group consisting of aluminum, platinum, or other materials that are chemically inert, electrically conductive, stable, and do not affect the reaction on other electrodes.

[0018] In another preferred embodiment, the third electrode comprises a platinum-glucose oxidase-gold thin film other electrode.

[0019] In another preferred embodiment, the fourth electrode comprises an electrode made of platinum, or other chemically inert, conductive, and stable materials that do not affect the reactions on other electrodes, serving as the counter electrode to the third electrode.

[0020] In another preferred embodiment, the reference electrode includes an electrode made of a material such as a silver-plated / silver chloride electrode or a mercury / mercury chloride electrode, which has electrode potential stability, good reproducibility, and undergoes a single reversible reaction.

[0021] In another preferred embodiment, the sensitive film element comprises a P3HT:PCBM blend film, or a sensitive film made of other readily film-forming organic semiconductor materials with good photosensitivity and photocurrent response.

[0022] In another preferred embodiment, the device for data recording and analysis includes devices such as computers and mobile phones that can be used for data signal processing.

[0023] In another preferred embodiment, in the light source module, the light emitted by the excitation light source is coupled to an optical lens through an optical fiber. The optical lens has a focusing function, enabling the light emitted by the excitation light source to be focused into a collimated beam, which may include an LED light source or a laser source.

[0024] In another preferred embodiment, the reaction module is used to hold the glucose liquid to be tested. It is the site where the glucose concentration and viscosity are measured, and the site where the enzymatic reaction occurs to measure the glucose concentration. It ensures that the glucose concentration response module and the glucose viscosity response module are in full contact with the sample to be tested, and at the same time protects the glucose concentration response module and the glucose viscosity response module and isolates them from external interference.

[0025] In another preferred embodiment, the shutter is connected to a timer, which controls the opening and closing of the shutter by generating a periodic signal, so that the light beam formed by the optical lens can periodically and perpendicularly illuminate the sensitive film element on the surface of the first electrode.

[0026] In another preferred embodiment, in the glucose viscosity response module, the first electrode and the second electrode are located on the substrate surface, and the sensitive film element is located on the first electrode.

[0027] In another preferred embodiment, the first electrode and the second electrode are fixed to the substrate surface by methods such as metal evaporation, chemical modification, or by an inert adhesive that does not affect the overall response.

[0028] In another preferred embodiment, the sensitive membrane element is fixed on the first electrode by methods such as solution drop coating, physical adsorption, or chemical modification.

[0029] In another preferred embodiment, the glucose viscosity response module is used to measure the viscosity of the glucose solution to be tested located in the reaction module.

[0030] In another preferred embodiment, the bottomless container is fixed to the substrate by an adhesive, such that the glucose viscosity response module and the glucose concentration response module are partially located inside the bottomless container.

[0031] In another preferred embodiment, the adhesive used to fix the bottomless container includes EVA or other substances with high strength, good barrier properties, and no impact on response, such as thermosetting or thermoplastic resins.

[0032] In another preferred embodiment, in the glucose concentration response module, the third electrode (e.g., a platinum-glucose oxidase-gold thin film working electrode), the fourth electrode (e.g., a platinum electrode), and the reference electrode (e.g., a silver-plated / silver chloride reference electrode) are located on the substrate surface. The metal (e.g., gold) on the third electrode is coated on the glucose oxidase layer in the form of a thin film, forming a platinum-glucose oxidase-gold thin film "sandwich" structure working electrode, and the glucose concentration response module is partially located in a bottomless container.

[0033] In another preferred embodiment, the third electrode, the fourth electrode, and the reference electrode are fixed to the substrate surface by methods such as metal evaporation, chemical modification, or by an inert adhesive that does not affect the overall response.

[0034] In another preferred embodiment, the glucose concentration response module is used to measure the concentration of the glucose solution to be tested located in the reaction module.

[0035] In another preferred embodiment, in the response measurement and analysis module, the measurement interface is connected to the glucose viscosity response module, the glucose concentration response module and the ammeter located outside the bottomless container, and the ammeter is connected to the data recording and analysis device through the data output port.

[0036] In another preferred embodiment, the response measurement and analysis module is used for subsequent data processing and analysis.

[0037] In another preferred embodiment, when measuring viscosity, the excitation light source in the light source module emits periodic light that passes through the glucose solution and irradiates the sensitive membrane element on the first electrode. A periodic photocurrent is generated between the first and second electrodes. By recording the measured current response, a time-current periodic response curve is plotted. The current response of multiple periods is integrated over time and then averaged to obtain the charge value passing through the solution. By performing multiple measurements on liquids with different viscosities, a standard curve of the charge value-viscosity relationship for each solution is obtained. Thus, the viscosity value of the solution is measured based on the charge value of the solution to be tested and the standard curve.

[0038] In another preferred embodiment, when measuring the concentration, an electric current is first applied to the gold film on the platinum-glucose oxidase-gold thin film working electrode to dissolve it, exposing glucose oxidase. The enzyme catalyzes the oxidation-reduction reaction of glucose in the solution to generate an electric current. The concentration of glucose in the test solution is then measured by recording the measured current response and based on the standard curve of the glucose current-concentration relationship.

[0039] In another preferred embodiment, the sensor first measures the glucose viscosity and then measures the glucose concentration. During viscosity measurement, the glucose concentration response module is not activated; during concentration measurement, the glucose viscosity response module is not activated.

[0040] It should be understood that, within the scope of this invention, the above-described technical features of this invention and the technical features specifically described below (such as in the embodiments) can be combined with each other to form new or preferred technical solutions. Due to space limitations, they will not be described in detail here. Attached Figure Description

[0041] Figure 1 This is a schematic diagram of the sensor system of the present invention.

[0042] Figure 2 This is a schematic diagram of the light source module in an embodiment of the present invention.

[0043] Figure 3 This is a schematic diagram of the glucose viscosity response module in an embodiment of the present invention.

[0044] Figure 4 This is a schematic diagram of the glucose concentration response module in an embodiment of the present invention.

[0045] Figure 5 This is a schematic diagram of the response measurement and analysis module in an embodiment of the present invention.

[0046] Figure 6 This is a schematic diagram of a specific embodiment of the present invention.

[0047] Figure 7 This is a schematic diagram of viscosity measurement example data in an embodiment of the present invention.

[0048] Figure 8 This is a schematic diagram of concentration measurement example data in an embodiment of the present invention.

[0049] Explanation of reference numerals in the attached figures:

[0050] 10: Light source module;

[0051] 20: Reaction module;

[0052] 30: Glucose viscosity response module;

[0053] 40: Glucose concentration response module;

[0054] 50: Response Measurement and Analysis Module;

[0055] 11: Laser source;

[0056] 12: Optical fiber;

[0057] 13: Optical lens;

[0058] 14: Shutter speed;

[0059] 15: Timer;

[0060] 21: Silicon dioxide substrate;

[0061] 22: Bottomless container;

[0062] 31: Gold electrode;

[0063] 32: Aluminum electrode;

[0064] 33: P3HT:PCBM blend membrane;

[0065] 41: Platinum-glucose oxidase-gold thin film working electrode;

[0066] 42: Platinum counter electrode;

[0067] 43: Silver-plated / silver chloride reference electrode;

[0068] 51: Measurement interface;

[0069] 52: Ammeter;

[0070] 53: Data recording and analysis equipment. Detailed Implementation

[0071] Through extensive and in-depth research, the inventors have developed, for the first time, a sensor capable of measuring the viscosity and concentration of glucose solutions. Viscosity measurement is based on the photoelectric effect of organic semiconductor materials, while concentration measurement is based on a biosensing detection mechanism based on enzyme-specific recognition. Using this sensor, the viscosity and concentration of glucose solutions can be measured in the same system. The sensor first measures the glucose viscosity and then the glucose concentration, avoiding the cumbersome steps of traditional methods that require separate measurements of glucose concentration and viscosity in their respective systems. This allows for faster response, more stable and reliable detection results, and higher efficiency while reducing the required sample volume (down to the order of 10-100 microliters). This invention is based on this foundation.

[0072] Sensor for measuring the viscosity and concentration of glucose solution

[0073] One aspect of the present invention aims to provide a sensor for measuring the viscosity and concentration of a glucose solution.

[0074] like Figure 1 As shown, the sensor of the present invention includes a light source module 10, a reaction module 20, a glucose viscosity response module 30, a glucose concentration response module 40, and a response measurement and analysis module 50. Further, as... Figure 2 As shown, the light source module 10 includes a laser source 11, an optical fiber 12, an optical lens 13, a shutter 14, and a timer 15; further, the reaction module 20 includes a silicon dioxide substrate 21 and a bottomless container 22; further, as... Figure 3 As shown, the glucose viscosity response module 30 includes a gold electrode 31, an aluminum electrode 32, and a P3HT:PCBM blend film 33; further, as Figure 4 As shown, the glucose concentration response module 40 includes a platinum-glucose oxidase-gold thin film working electrode 41, a platinum counter electrode 42, and a silver-plated / silver chloride reference electrode 43; further, as Figure 5 As shown, the response measurement and analysis module 50 includes a measurement interface 51, a galvanometer 52, and a data recording and analysis device 53 (computer, mobile phone, etc.).

[0075] Furthermore, such as Figure 6The diagram shows an overall design of a sensor based on the present invention capable of measuring the viscosity and concentration of a glucose solution. The excitation light emitted by the laser source 11 is coupled to an optical lens 13 via an optical fiber 12. The optical lens 13 has a focusing function, enabling the excitation light emitted by the laser source 11 to be focused into an excitation beam. Furthermore, the shutter 14 is connected to a timer 15, which generates periodic signals to control the opening and closing of the shutter 14, allowing the excitation beam formed by the optical lens 13 to periodically and perpendicularly irradiate the P3HT:PCBM blend film 33. Furthermore, the gold electrode 31 and aluminum electrode 32 are grown on the surface of the silicon dioxide substrate 21, and the P3HT:PCBM blend film 33 is only coated on the gold electrode 31; the bottomless container 22 is fixed to the silicon dioxide substrate 21 by an adhesive such as EVA, so that the glucose viscosity response module 30 is partially located inside the bottomless container 22; furthermore, the platinum-glucose oxidase-gold thin film working electrode 41, platinum counter electrode 42, and silver-plated / silver chloride reference electrode 43 are located on the surface of the silicon dioxide substrate 21, gold is coated on the glucose oxidase layer in the form of a thin film, and glucose oxidase is fixed on the platinum electrode, forming a platinum-glucose oxidase-gold thin film "sandwich" structure. The working electrode is positioned such that the glucose concentration response module 40 is located within the bottomless container 22; further, the test solution is added into the bottomless circular container 22, such that the gold electrode 31, aluminum electrode 32, P3HT:PCBM blend film 33, and platinum-glucose oxidase-gold thin film working electrode 41, platinum counter electrode 42, and silver-plated / silver chloride reference electrode 43 are covered and connected by the test solution; further, the glucose viscosity response module 30, the glucose concentration response module 40, and the ammeter 52 located outside the bottomless container 22 are connected through the measurement interface 51, and the ammeter 52 is connected to the data recording and analysis device 53 through the data output port.

[0076] Furthermore, subsequent data processing and analysis are performed through the response measurement and analysis module 50. When measuring viscosity, the laser source is turned on. At this time, the P3HT:PCBM blend film is excited by the excitation light to generate electrons, thereby generating photocurrent. By controlling the shutter switch through a timer (or manually), a periodic current change can be generated on the electrode. This periodic change is collected and displayed on the device through the response measurement and analysis module 50. By recording the measured current response and plotting it as a time-current periodic response curve, integrating the current response over multiple periods and then averaging the results, the charge value of the solution can be obtained. By performing multiple measurements on liquids of different viscosities, a standard curve of the charge-viscosity relationship for each solution is obtained. Based on the charge value of the sample to be tested and the standard curve, the viscosity value of the solution can be measured. At this point, since the gold film on the platinum-glucose oxidase-gold thin film working electrode 41 has not yet dissolved under current, only the viscosity response exists, thus enabling the solution viscosity to be measured first. After the viscosity measurement is completed, when the concentration is measured, the platinum-glucose oxidase-gold thin film working electrode 41 is reverse-current energized through the response measurement and analysis module 50, causing the gold film to dissolve. At this point, the glucose oxidase on the electrode is exposed, catalyzing the oxidation and decomposition of glucose in the solution to be tested to generate electrons and thus generate current. The current response is collected and displayed on the device by the response measurement and analysis module 50. By performing multiple measurements on glucose solutions of varying concentrations, a standard curve of the current-concentration relationship of glucose was obtained. Then, by recording the current response of the test solution and plotting it as a time-current response curve, the concentration of glucose in the test solution could be measured.

[0077] The main advantages of this invention include:

[0078] (1) The sensor of the present invention measures glucose concentration and viscosity sequentially within the same system. Viscosity measurement utilizes the different current responses generated by the photosensitive device under different solution conditions, thereby enabling relatively accurate indirect measurement of the viscosity of the solution to be tested, ensuring the reliability of viscosity measurement; concentration measurement is achieved through the specific identification of glucose by glucose oxidase. Traditional concentration and viscosity measurements require separate measurements in two systems.

[0079] (2) The sensor of the present invention does not require the use of a relatively precise moving part to measure viscosity, thus eliminating the interference of external motion factors; at the same time, the measurement can be performed simply by covering the electrode with the liquid to be measured to form a circuit, which greatly reduces the volume of the liquid to be measured and reduces the cost. Compared with general viscosity measurement, it simplifies the more demanding measurement conditions.

[0080] (3) The sensor of the present invention greatly reduces the size of the measurement system and improves the portability of the system. It can be used with mobile devices to achieve portable measurement and the measurement process is more efficient.

[0081] (4) The sensor of the present invention measures the glucose concentration in the glucose solution after measuring the viscosity of the glucose solution, and avoids the interference of the new substances generated by the oxidation reaction of glucose when measuring the concentration first on the subsequent glucose solution viscosity. It can be used with mobile devices to realize portable measurement of glucose concentration and viscosity.

[0082] (5) After the sensor of the present invention is initially adjusted and debugged in the response measurement and analysis module 50, subsequent measurements only require manual operation of the laser source and reverse power control of the gold thin film, so it is easy to learn and simple to operate.

[0083] The invention will be further illustrated below with reference to specific embodiments. It should be understood that these embodiments are for illustrative purposes only and are not intended to limit the scope of the invention. Experimental methods in the following embodiments, unless otherwise specified, are generally performed under conventional conditions, such as those recommended by the manufacturer. Unless otherwise stated, percentages and parts are by weight.

[0084] Unless otherwise specified, all materials and reagents used in the embodiments of this invention are commercially available products.

[0085] Example

[0086] like Figure 1 As shown, the sensor of the present invention includes a light source module 10, a reaction module 20, a glucose viscosity response module 30, a glucose concentration response module 40, and a response measurement and analysis module 50. Further, as... Figure 2 As shown, the light source module 10 includes a laser source 11, an optical fiber 12, an optical lens 13, a shutter 14, and a timer 15; further, the reaction module 20 includes a silicon dioxide substrate 21 and a bottomless container 22; further, as... Figure 3 As shown, the glucose viscosity response module 30 includes a first electrode (gold electrode 31), a second electrode (aluminum electrode 32), and a P3HT:PCBM blend film 33; further, as Figure 4 As shown, the glucose concentration response module 40 includes a third electrode (platinum-glucose oxidase-gold thin film working electrode 41), a fourth electrode (platinum counter electrode 42), and a reference electrode 43 (silver-plated / silver chloride electrode); further, as Figure 5 As shown, the response measurement and analysis module 50 includes a measurement interface 51, a galvanometer 52, and a data recording and analysis device 53 (such as a computer, mobile phone, or other device).

[0087] Furthermore, the gold electrode 31, aluminum electrode 32, platinum-glucose oxidase-gold thin film working electrode 41, platinum counter electrode 42, and silver-plated / silver chloride reference electrode 43 are arranged in parallel in the reaction module (P3HT:PCBM blend film 33 is located on the gold electrode 31, the light source module is located directly above the gold electrode 31, and the response measurement and analysis module is connected to the glucose viscosity response module and the glucose concentration response module through measurement interfaces respectively, while the glucose viscosity response module and the glucose concentration response module are not connected to each other).

[0088] Furthermore, such as Figure 6The diagram illustrates an overall design of a sensor based on the photoelectric response of this invention, capable of measuring the viscosity and concentration of a glucose solution. The excitation light emitted by the laser source 11 is coupled to an optical lens 13 via an optical fiber 12. The optical lens 13 has a focusing function, enabling the excitation light emitted by the laser source 11 to be focused into an excitation beam. In a preferred embodiment, the laser source in the light source module can emit light of a specific wavelength, the wavelength of which belongs to the absorption wavelength of the P3HT:PCBM blend film 33. Furthermore, the shutter 14 is connected to a timer 15, which controls the opening and closing of the shutter 14 by generating a periodic signal, allowing the excitation beam formed by the optical lens 13 to periodically and perpendicularly irradiate the P3HT:PCBM blend film 33. Furthermore, the gold electrode 31 and aluminum electrode 32 are fixed to the surface of the silica substrate 21 by metal vapor deposition (metal vapor deposition is a specific method, which can be directly placed into the machine for metal vapor deposition). The P3HT:PCBM blend film 33 is only coated on the gold electrode 31. The bottomless container 22 is fixed to the silica substrate 21 by an adhesive such as EVA, so that the glucose viscosity response module 30 is partially located inside the bottomless container 22. Furthermore, the platinum-glucose oxidase-gold thin film working electrode 41 is fixed to the surface of the silica substrate 21 by three methods: metal vapor deposition, physical adsorption, and chemical modification; the platinum counter electrode 42 is fixed by metal vapor deposition; and the silver / silver chloride reference electrode 43 is fixed by first metal vapor deposition and then chemical modification. For the platinum-glucose oxidase-gold thin film working electrode 41, platinum metal is first vapor-deposited, and then the glucose oxidase layer is fixed by physical adsorption. (Achieved by adding adsorbent materials such as chitosan and carbon nanotubes) adsorbed onto the platinum layer, and finally, gold is chemically modified to cover the glucose oxidase layer in the form of a thin film, forming a platinum-glucose oxidase-gold thin film "sandwich" structure working electrode, and making the glucose concentration response module 40 part located in the bottomless container 22; further, the test solution is added into the bottomless circular container 22, so that the gold electrode 31, aluminum electrode 32, P3HT:PCBM blend film 33, and platinum-glucose oxidase-gold thin film working electrode 41, platinum counter electrode 42 and reference electrode 43 (silver-plated / silver chloride electrode) are covered and connected by the test solution; further, the glucose viscosity response module 30 part, the glucose concentration response module 40 part and the ammeter 52 located outside the bottomless container 22 are connected through the measurement interface 51, and the ammeter 52 is connected to the data recording and analysis device 53 through the data output port.

[0089] Furthermore, the response measurement and analysis module 50 performs subsequent data processing and analysis. When measuring viscosity, the laser source is turned on. At this time, the P3HT:PCBM blend film is excited by the excitation light to generate electrons, thereby generating photocurrent. By controlling the shutter switch through a timer (or manually), a periodic current change can be generated on the electrode. This periodic change is collected and displayed on a device (such as a mobile phone, computer, etc.) by the response measurement and analysis module 50. The measured current response is recorded and plotted as a time-current periodic response curve. The current response of multiple periods is integrated and then averaged to obtain the charge value of the solution. By performing multiple measurements on liquids with different viscosities, a standard curve of the charge-viscosity relationship for each solution is obtained. The viscosity value of the solution is then measured based on the charge value of the sample and the standard curve. At this point, since the gold film on the platinum-glucose oxidase-gold thin film working electrode 41 has not yet dissolved under current, only the viscosity response exists. Therefore, the viscosity of the solution is measured first. When the concentration is measured after the viscosity measurement is completed, the platinum-glucose oxidase-gold thin film working electrode 41 is reverse-currented through the response measurement and analysis module 50 to dissolve the gold film. At this time, the glucose oxidase on the electrode is exposed, catalyzing the oxidation and decomposition of glucose in the solution to generate electrons and thus generate current. The current response is collected and displayed on the device by the response measurement and analysis module 50. By performing multiple measurements on glucose solutions of varying concentrations, a standard curve of the current-concentration relationship of glucose was obtained. Then, by recording the current response of the test solution and plotting it as a time-current response curve, the concentration of glucose in the test solution could be measured.

[0090] In this invention, a specific measurement example process is as follows:

[0091] A certain volume (e.g., 1 mL) of a test solution (containing glucose) of varying viscosities is added to the bottomless container 22. The light source module 10 is activated, generating an excitation beam with an 8-second period (5 seconds on, 3 seconds off) that irradiates the glucose viscosity response module 30. At this time, the P3HT:PCBM blend film is excited by the excitation light, generating photoelectrons and thus a photocurrent. This causes a periodic current change on the electrode, which is collected and displayed on a device (e.g., a mobile phone or computer) by the response measurement and analysis module 50. Data recording and analysis by the device 53 (e.g., a mobile phone or computer) yields results such as... Figure 7The time-current periodic response curve shown (current data and time are plotted as an image using conventional methods) allows for the determination of solution viscosity by comparing the charge value calculated from the integral of the response curve with the charge-viscosity relationship curve of the solution. After viscosity measurement, an electric current is applied to the gold film on the platinum-glucose oxidase-gold thin film working electrode 41 to dissolve it, exposing the glucose oxidase on the platinum electrode. A glucose solution of a certain concentration (e.g., 0.1 mol / L) is then added dropwise to the solution. The glucose oxidase catalyzes the oxidation and decomposition of glucose in the test solution, generating electrons and thus current. The current response is acquired and displayed on a device (e.g., a mobile phone or computer) through the response measurement and analysis module 50. Data recording and analysis devices 53 (e.g., mobile phones or computers) can then be used to obtain data such as... Figure 8 The time-current response curve shown (current data and time are plotted as a graph using conventional methods) allows us to determine the glucose concentration by comparing the magnitude of the response value with the glucose current-concentration relationship curve.

[0092] As can be seen from the above, its response is obvious and can accurately reflect the relationship between the charge and the viscosity, current and concentration of the glucose solution. It enables the measurement of the glucose concentration after measuring the viscosity of the glucose solution, and avoids the interference caused by the oxidation reaction of glucose when measuring the concentration first.

[0093] All documents mentioned in this invention are incorporated herein by reference as if each document were individually incorporated by reference. Furthermore, it should be understood that after reading the foregoing teachings of this invention, those skilled in the art can make various alterations or modifications to this invention, and these equivalent forms also fall within the scope defined by the appended claims.

Claims

1. A sensor for measuring the viscosity and concentration of a glucose solution, characterized in that, include: The system comprises a light source module, a reaction module, a glucose viscosity response module, a glucose concentration response module, and a response measurement and analysis module. The light source module includes an excitation light source, an optical fiber, an optical lens, a shutter, and a timer; the reaction module includes a substrate and a bottomless container. The glucose viscosity response module includes a first electrode, a second electrode, and a sensitive membrane element; The glucose concentration response module includes a third electrode, a fourth electrode, and a reference electrode; The response measurement and analysis module includes a measurement interface, a galvanometer, and equipment for data recording and analysis. Furthermore, the first electrode, second electrode, third electrode, fourth electrode, and reference electrode are arranged in parallel in the reaction module, the sensitive membrane element is located on the first electrode, the light source module is located directly above the first electrode, and the response measurement and analysis module is connected to the glucose viscosity response module and the glucose concentration response module, respectively. The glucose viscosity response module and the glucose concentration response module are not connected to each other. The third electrode includes a platinum-glucose oxidase-gold thin film working electrode; When measuring the concentration, an electric current is first applied to the gold film on the platinum-glucose oxidase-gold thin film working electrode to dissolve it and expose glucose oxidase. The enzyme catalyzes the oxidation reaction of glucose in the solution to generate an electric current. The concentration of glucose in the test solution is then measured by recording the measured current response and based on the standard curve of the current-concentration relationship of glucose. The sensor first measures the glucose viscosity, and then measures the glucose concentration; the glucose concentration response module does not work during viscosity measurement, and the glucose viscosity response module does not work during concentration measurement.

2. The sensor as described in claim 1, characterized in that, The sensitive membrane element is an organic semiconductor material, which is fixed onto the first electrode by one or more methods selected from the group consisting of: solution drop coating, physical adsorption, and / or chemical modification.

3. The sensor as described in claim 1, characterized in that, The light source module excites the light source to emit light of a specific wavelength, which is the absorption wavelength of the sensitive film element.

4. The sensor as described in claim 1, characterized in that, The first electrode comprises an electrode made of one or more materials selected from the group consisting of gold.

5. The sensor as described in claim 1, characterized in that, The second electrode comprises an electrode made of one or more materials selected from the group consisting of aluminum and platinum.

6. The sensor as described in claim 1, characterized in that, The fourth electrode comprises a platinum electrode, or an electrode made of other chemically inert, conductive, and stable materials that do not affect the reactions on other electrodes, serving as the counter electrode to the third electrode.

7. The sensor as described in claim 1, characterized in that, The reference electrode includes a silver-plated / silver chloride electrode or a mercury / mercuric chloride electrode.

8. The sensor as described in claim 1, characterized in that, When measuring viscosity, the excitation light source in the light source module emits periodic light that irradiates the sensitive membrane element on the first electrode. A periodic photocurrent is generated between the first and second electrodes. By recording the measured current response, a time-current periodic response curve is plotted. The current response of multiple periods is integrated over time and then averaged to obtain the charge value of the solution. By performing multiple measurements on liquids with different viscosities, a standard curve of the charge value-viscosity relationship for each solution is obtained. Thus, the viscosity value of the solution to be tested can be measured based on the charge value of the solution to be tested and the standard curve.

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