Low-temperature plasma fusion ultraviolet special pathogenic bacteria and new coronavirus disinfection technology device

Abstract

The present invention discloses a low-temperature plasma fusion ultraviolet special pathogen coronavirus disinfection technology device, which belongs to the field of disinfection technology. The device includes a low-temperature plasma airflow generator, an ultraviolet generator and an airflow circulation system; the ultraviolet generator is located in front of the low-temperature plasma airflow, and the disinfection airflow formed by the fusion of the low-temperature plasma airflow and ultraviolet light is sprayed onto the surface of the sample to be disinfected, thereby disinfecting the pathogens and coronaviruses on the sample surface; the disinfection airflow circulation is used to improve the disinfection efficiency and reduce O3 emissions. Using a working voltage of 33±5kV, a frequency of 13±5kHz, and a fusion of ultraviolet light with a wavelength of 222±10 nanometers, 6Log10CFU / cm2 of Staphylococcus aureus is disinfected and killed in 160s, and 4.28log10TCID50 / cm2 of coronavirus (SARS-CoV-2) is disinfected and killed in 30s. The efficient disinfection efficiency of this technical device for microbial coronaviruses will provide core technical device support for the development of special equipment for disinfection of pathogens and coronaviruses in modern cold chain logistics.

Description

Technical Field

[0001] The present invention relates to the technical field of pathogen and coronavirus disinfection, and in particular to a low-temperature plasma fusion ultraviolet special pathogen and coronavirus disinfection technology device. Background Art

[0002] In April 2021, the National Health Commission issued the "Diagnosis and Treatment Plan for Coronavirus Pneumonia (Trial Eighth Edition)", which pointed out that "Coronavirus is sensitive to ultraviolet rays and heat. 56°C for 30 minutes, ether, 75% ethanol, chlorine-containing disinfectants, peracetic acid, chloroform and other lipid solvents can effectively inactivate the virus", which mainly includes two basic methods: chemical disinfection and physical disinfection. Chemical disinfection mainly uses disinfectants containing active ingredients such as chlorine and peroxides for spraying or soaking to achieve the purpose of disinfection. However, due to the low temperature of the cold chain transportation environment, the disinfectant sprayed in mist form will frost and ice on the surface of the treated object, affecting the disinfection effect, and chemical agents have certain hazards to human health, which seriously affects the health of cold chain logistics practitioners. Physical disinfection is typically based on ultraviolet irradiation, among which 253.7nm ultraviolet light has the most ideal effect on pathogens, but long-term exposure may cause skin cancer and affect personal safety. In 2020, the 222nm ultraviolet lamp developed by Columbia University in the United States cannot penetrate the skin surface and eyes, is relatively safe for the human body, and has a certain disinfection effect on pathogens and coronaviruses. The University of Duisburg-Essen in Germany used long-wave 365nm (irradiation dose of 500mJ / cm2) ultraviolet light to treat 6.69log10TCID50 / mL SARS-CoV-2 for 15 minutes, which could only reduce 2 logs, while short-wave 254nm (irradiation dose of 500mJ / cm2) treatment for 6 minutes could reduce 4.5 logs. The Department of Virology at Hiroshima University in Japan used 222nm short-wave ultraviolet light (irradiation dose of 30mJ / cm2) to treat 6.32log10TCID50 / mL coronavirus (SARS-CoV-2) for 5 minutes, which can reduce 2.51 logs, but cannot completely kill it. Summary of the Invention

[0003] The present invention provides a low-temperature plasma-fused ultraviolet (UV)-specific pathogenic coronavirus disinfection device, comprising a low-temperature plasma airflow generator, an UV generator, and an airflow circulation system. The UV generator is positioned in front of the low-temperature plasma airflow. The disinfection airflow, generated by the fusion of the low-temperature plasma airflow and 222-nanometer ultraviolet light, is sprayed onto the surface of the sample being disinfected, thereby disinfecting the pathogens or coronaviruses on the sample surface. The airflow circulation system simultaneously recycles the airflow, improving disinfection performance and reducing O3 emissions.

[0004] The purpose of the present invention can be achieved through the following technical solutions:

[0005] A low-temperature plasma fusion ultraviolet special pathogen and coronavirus disinfection technology device is characterized in that: the technical device includes a low-temperature plasma airflow generator, an ultraviolet generator and an airflow circulation system; the ultraviolet generator is located in front of the low-temperature plasma airflow, and the disinfection airflow formed by the fusion of the low-temperature plasma airflow and ultraviolet light is sprayed onto the surface of the sample to be disinfected, thereby disinfecting the pathogens and coronaviruses on the sample surface.

[0006] In the above device: the low-temperature plasma airflow generator includes an electrode tube assembly, an electrode tube frame, a micro fan and a fan cover frame; the electrode tube frame is provided with 2-5 layers of ceramic electrode tubes, the electrode tubes are arranged crosswise or in parallel, and the upper and lower and left and right spacing L of the electrode tubes is 6±2mm; the two ends of the electrode tubes are connected together outside the electrode frame and connected to the two poles of the high-voltage power supply.

[0007] In the above device: the ultraviolet generator consists of 2-5 groups of ultraviolet light sources, which are evenly distributed in front of the low-temperature plasma airflow plane. The height of the sample to be processed from the center line plane of the ultraviolet light source is 4-5 cm, and the distance between the sample to be processed and the plasma airflow outlet surface is 8-12 cm.

[0008] In the above device: the air flow circulation system includes an axial flow fan, a gas pipeline, and a flow sensor. The gas flow sensor-PLC is used to control the axial flow fan to regulate the low-temperature plasma air flow.

[0009] In the above device, the micro fans provided in the low-temperature plasma airflow generator are evenly distributed on the fan cover frame, and the distance h2 between the fan plane and the center plane of the upper electrode tube is 20±5mm; the air flow rate of the micro fans is 1-8m 3 / min.

[0010] In the above device, the ceramic electrode tube consists of a ceramic shell, a metal powder filling, and a silicone seal. The ceramic shell is composed of 99.5% Al2O3, has an outer diameter of 15-25mm, and a wall thickness of 1-3mm. The silicone seals at both ends are made of SiO2, and a copper or aluminum wire with a diameter of 2±0.5mm extends through the center of the silicone seal.

[0011] In the above device: the ceramic tube is filled with metal powder, which consists of aluminum powder, magnesium powder, copper powder and titanium powder, with the weight proportions of each component being 30-60 parts, 30-60 parts, 10-30 parts and 0.1-5 parts respectively, and the particle size of the metal powder is 80-150 mesh.

[0012] A method for disinfecting pathogens and coronaviruses using the above-mentioned device is characterized in that: a low-temperature plasma fusion ultraviolet disinfection airflow formed by the fusion excitation of a low-temperature plasma airflow and ultraviolet light is sprayed onto the surface of the sample to be disinfected for 30 seconds to 150 seconds, thereby disinfecting pathogens and / or coronaviruses on the sample surface; the disinfection airflow is simultaneously recycled through an airflow circulation system to improve disinfection efficiency and reduce O3 emissions.

[0013] In the above method, the operating voltage of the low-temperature plasma airflow generator is 10-40 kV, the operating frequency is 8-20 kHz, and the low-temperature plasma power density is 0.5-2.5 W / cm 2 The UV light generated by the UV generator has a wavelength of 222 ± 10 nm and a power density of 0.25-0.65 W / cm 2 .

[0014] Beneficial effects of the present invention:

[0015] (1) 222nm ultraviolet is a new technology for efficient and safe disinfection of coronavirus that has just been launched internationally. The present invention combines 222nm far ultraviolet technology with low-temperature plasma technology to form a new technology and equipment for efficient physical field disinfection of coronavirus that is harmless to the human body. It can achieve efficient and short-term killing of coronavirus, and provides an original technical equipment support for the "efficient, green and low-carbon" disinfection of coronavirus in large-scale and high-throughput modern logistics automation.

[0016] (2) Low-temperature plasma is integrated with 222nm ultraviolet. On the basis of maintaining the respective unique advantages of low-temperature plasma airflow and 222nm ultraviolet disinfection, the fusion of the two significantly improves the disinfection efficiency of pathogens and coronaviruses, and makes up for their respective shortcomings. The "low-temperature plasma fusion ultraviolet special pathogen coronavirus disinfection technology device" created can better adapt to the disinfection of coronaviruses in specific scenarios such as cold chain logistics, airport customs luggage and express logistics.

[0017] (3) The present invention can optimize the intensity of the low-temperature plasma fusion 222nm ultraviolet disinfection airflow, reduce energy consumption, and improve disinfection efficiency and reduce O3 emissions by regulating technical parameters such as the voltage intensity, operating frequency, airflow velocity and 222nm ultraviolet power density of the high-voltage electric field low-temperature plasma. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1 This is a diagram of a low-temperature plasma fusion ultraviolet-specific pathogen and coronavirus disinfection technology device.

[0019] Figure 2 It is a low-temperature plasma airflow device.

[0020] Figure 3 Schematic diagram of the electrode tube structure.

[0021] Among them: 1 is a micro fan, 2 is a fan cover frame, 3 is a ceramic electrode tube, 4 is a tube-type electrode frame, 5 is a UV lamp tube, 6 is a UV frame, 7 is an implementation sample, 8 is an axial flow fan, 9 is a flow sensor, 10 is a pipeline, 11 is a fan power supply, 12 is a low-temperature plasma power supply, 13 is a 222 nm UV power supply, 14 is a PLC controller, 15 is a silicone seal, 16 is a ceramic tube shell, 17 is metal powder; 18 is a wire. DETAILED DESCRIPTION

[0022] The present invention will be further described below with reference to the embodiments, but the protection scope of the present invention is not limited thereto:

[0023] like Figures 1 to 3 A low-temperature plasma-fused ultraviolet disinfection device for pathogens and coronaviruses includes a low-temperature plasma airflow generator, an ultraviolet generator, and an airflow circulation system. The ultraviolet generator is located in front of the low-temperature plasma airflow. The low-temperature plasma airflow and ultraviolet light fusion excite the low-temperature plasma + ultraviolet disinfection airflow, which is sprayed onto the surface of the sample to be disinfected, disinfecting the pathogens and / or coronaviruses on the sample surface. At the same time, the airflow is recycled through the airflow circulation system to improve the disinfection function and reduce O3 emissions.

[0024] The low-temperature plasma airflow generator includes an electrode tube assembly, an electrode tube frame, a micro-blower, and a blower cover. The electrode tube frame houses two layers of ceramic electrode tubes, each layer containing eight electrode tubes. The two layers of connected electrode tubes are arranged crosswise or in parallel. The vertical and horizontal spacing (L) of the electrode tubes is 6±2 mm. The ends of the electrode tubes are connected together outside the electrode frame and connected to the two poles of a high-voltage power supply.

[0025] The UV generator consists of two sets of UV light sources, evenly distributed in front of the low-temperature plasma airflow plane. The airflow circulation system includes an axial flow fan, gas pipeline, and flow sensor. The gas flow sensor-PLC controls the axial flow fan to regulate the low-temperature plasma airflow.

[0026] The micro fans set in the low-temperature plasma airflow generator are evenly distributed on the fan cover frame. The distance h2 between the fan plane and the center plane of the upper electrode tube is 22 mm. The air flow rate of the micro fans is 4 to 5 m 3 / min.

[0027] The ceramic electrode tube consists of a ceramic shell, a metal powder filling, and a silicone seal. The ceramic shell contains 99.5% Al2O3, has an outer diameter of 20mm, and a wall thickness of 2mm. The silicone seals at both ends are made of SiO2, and copper or aluminum wires with a diameter of 2±0.5mm extend through the center of the silicone seals. The ceramic tube is filled with metal powder, which consists of aluminum powder, magnesium powder, copper powder, and titanium powder in the proportions of 40 parts by weight, 40 parts by weight, 19 parts by weight, and 1 part by weight, respectively. The particle size of the metal powder is 100-120 mesh. The tube-type electrode frame and UV frame are both made of polytetrafluoroethylene.

[0028] The method for sterilization using the above-mentioned device is as follows: the disinfection airflow formed by the fusion of low-temperature plasma airflow and ultraviolet light is sprayed onto the surface of the sample to be treated, and the treatment is carried out for 30 seconds to 150 seconds to disinfect the pathogens and / or coronaviruses on the surface of the sample; the disinfection airflow is simultaneously recycled through the airflow circulation system to improve the disinfection efficiency and reduce O3 emissions.

[0029] The specific cases are as follows:

[0030] Example 1: Effect of different high-voltage electric field operating frequencies on the sterilization of pathogens by low-temperature plasma fusion with 222nm ultraviolet light

[0031] Pipette 10 μL of 10 8 CFU / mL Staphylococcus aureus (Gram-positive bacteria) suspension was dropped onto a 1 cm × 1 cm quartz glass slide (after degreasing and disinfection) and evenly spread over a 1 cm 2 The dried glass pieces were subjected to different treatments (the sample was 9±0.5 cm away from the plasma airflow outlet and 4.3±0.2 cm above the centerline plane of the UV light source): ① low-temperature plasma (30 s) followed by 222 nm UV treatment (30 s), ② 222 nm UV treatment (30 s) followed by low-temperature plasma (30 s), ③ low-temperature plasma (60 s) followed by 222 nm UV treatment (60 s), ④ 222 nm UV treatment (60 s) followed by low-temperature plasma (60 s), ⑤ low-temperature plasma fusion and 222 nm UV integration (30 s, device of the present invention), and ⑥ low-temperature plasma fusion and 222 nm UV integration (60 s, device of the present invention), respectively labeled as CP+UV-1, UV+CP-1, CP+UV-2, UV+CP-2, UV-CP-1 integration, and UV-CP-2 integration. The untreated group served as the control group. The treated group had a low-temperature plasma gas flow generator operating voltage of 33 ± 5 kV and a high-voltage electric field operating frequency of 8.5 kHz and 19 kHz, respectively. Treatment parameters: low-temperature plasma power density of 2.5 W / cm 2 , UV power density 0.6W / cm 2The UV generator has a wavelength of 222nm. Two layers of ceramic electrode mesh are arranged in parallel. The antibacterial effect was determined using the plate count method.

[0032] Table 1 Effects of different high voltage electric field operating frequencies on Staphylococcus aureus

[0033]

[0034] As can be seen from Table 1, the number of Staphylococcus aureus in the control group was 6.41±0.12Log 10 CFU / cm 2 After different treatments, in the same group: the UV-CP-1 integrated group was always lower than the CP+UV-1 and UV+CP-1 groups; the UV-CP-2 integrated group was always lower than the CP+UV-2 and UV+CP-2 groups, indicating that the sterilization effect of the low-temperature plasma fusion 222nm ultraviolet integrated treatment was significantly higher than that of the sequential treatment groups; in different groups: the number of Staphylococcus aureus of 8.5kHz and 19kHz UV-CP-2 integrated treatments were 1.78±0.23 and 1.83±0.12Log respectively 10 CFU / cm 2 , killing 4.63 and 4.58 Log values ​​respectively, and there was no significant difference between the high-voltage electric field operating frequency of 8.5kHz and 19kHz (p>0.05). Therefore, the high-voltage electric field operating frequency of 8.5kHz was preferred for the following experiments.

[0035] Example 2: Effect of different power densities of low-temperature plasma combined with 222nm ultraviolet light on the elimination of Staphylococcus aureus

[0036] Pipette 10 μL of 10 8 CFU / mL Staphylococcus aureus (Gram-positive bacteria) suspension was dropped onto a 1 cm × 1 cm quartz glass slide (after degreasing and disinfection) and evenly spread over a 1 cm 2The dried glass slides were then subjected to different treatments (the sample was 9 ± 0.5 cm from the plasma airflow outlet and 4.3 ± 0.2 cm from the centerline of the UV light source): ① low-temperature plasma (30 s) followed by 222 nm UV (30 s), ② 222 nm UV (30 s) followed by low-temperature plasma (30 s), ③ low-temperature plasma (60 s) followed by 222 nm UV (60 s), ④ 222 nm UV (60 s) followed by low-temperature plasma (60 s), ⑤ low-temperature plasma fusion with 222 nm UV (30 s), and ⑥ low-temperature plasma fusion with 222 nm UV (60 s). These treatments are labeled CP+UV-1, UV+CP-1, CP+UV-2, UV+CP-2, UV-CP-1 integration, and UV-CP-2 integration, respectively. The untreated group was the control group, and the treated groups had low-temperature plasma power densities of 0.5, 1.0, 1.5, 2.0, and 2.5 W / cm 2 Treatment parameters: The operating voltage of the low-temperature plasma gas flow generator is 33±5kV, the operating frequency of the high-voltage electric field is 8.5kHz, the wavelength of the ultraviolet generator is 222nm, and the ultraviolet power density is 0.6W / cm 2 The ceramic electrode mesh is arranged in parallel and has two layers. The antibacterial effect is determined by the plate count method.

[0037] Table 2 Effect of low-temperature plasma power density on Staphylococcus aureus

[0038]

[0039] As can be seen from Table 2, the number of Staphylococcus aureus in the control group was 6.41±0.07Log 10 CFU / cm 2 After different treatments, in the same group: the UV-CP-1 integrated group was always lower than the CP+UV-1 and UV+CP-1 groups; the UV-CP-2 integrated group was always lower than the CP+UV-2 and UV+CP-2 groups, indicating that the sterilization effect of the low-temperature plasma fusion 222nm ultraviolet integrated treatment was significantly higher than that of the sequential treatment groups; in different groups: the power density was 0.5, 1.0, 1.5, 2.0 and 2.5 W / cm 2 The numbers of Staphylococcus aureus in UV+CP were 2.47±0.34, 2.35±0.24, 1.73±0.16, 1.47±0.04 and 1.09±0.10Log, respectively. 10 CFU / cm 2 , respectively killing 3.94, 4.05, 4.67, 4.93 and 5.31 Log values. As the power density increases, the sterilization effect becomes stronger. Therefore, the optimal low-temperature plasma power density is 2.5W / cm2 Carry out the next experiment.

[0040] Example 3: Experimental study on the disinfecting effect of low-temperature plasma fusion with 222nm ultraviolet light on Staphylococcus aureus

[0041] Pipette 10 μL of 10 8 CFU / mL Staphylococcus aureus (Gram-positive bacteria) suspension was dropped onto a 1 cm × 1 cm quartz glass slide (after degreasing and disinfection) and evenly spread over a 1 cm 2 The dried glass slides were then subjected to different treatments (the sample was 9 ± 0.5 cm from the plasma airflow outlet and 4.3 ± 0.2 cm from the centerline of the UV light source): ① low-temperature plasma (30 s) followed by 222 nm UV (30 s), ② 222 nm UV (30 s) followed by low-temperature plasma (30 s), ③ low-temperature plasma (60 s) followed by 222 nm UV (60 s), ④ 222 nm UV (60 s) followed by low-temperature plasma (60 s), ⑤ low-temperature plasma fusion with 222 nm UV (30 s), and ⑥ low-temperature plasma fusion with 222 nm UV (60 s). These treatments are labeled CP+UV-1, UV+CP-1, CP+UV-2, UV+CP-2, UV-CP-1 integration, and UV-CP-2 integration, respectively. The untreated group was the control group, and the treated group: the wavelength of the UV generator was 222 nm, and the UV power density was 0.2, 0.4, and 0.6 W / cm 2 Treatment parameters: The operating voltage of the low-temperature plasma gas flow generator is 33±5kV, the high-voltage electric field operating frequency is 8.5kHz, and the low-temperature plasma power density is 2.5W / cm 2 The ceramic electrode mesh is arranged in parallel and has two layers. The antibacterial effect is determined by the plate count method.

[0042] Table 3 Effects of different UV power densities on Staphylococcus aureus

[0043]

[0044] As can be seen from Table 3, the number of Staphylococcus aureus in the control group was 6.38±0.32Log 10 CFU / cm 2 After different treatments, in the same group: the UV-CP-1 integrated group was always lower than the CP+UV-1 and UV+CP-1 groups; the UV-CP-2 integrated group was always lower than the CP+UV-2 and UV+CP-2 groups, indicating that the sterilization effect of the low-temperature plasma fusion 222nm ultraviolet integrated treatment was significantly higher than that of the sequential treatment groups; in different groups: 0.2, 0.4 and 0.6W / cm 2The number of Staphylococcus aureus in UV+CP was 2.24±0.14, 2.18±0.06 and 1.76±0.05Log, respectively. 10 CFU / cm 2 , killing 4.14, 4.20 and 4.62 Log values ​​respectively, and the UV power density was 0.2 and 0.4 W / cm 2 There was no significant difference (p>0.05), but they were the same as the UV power density of 0.6W / cm 2 There is a significant difference (p < 0.05). Therefore, the optimal UV power density is 0.6W / cm 2 Carry out the next experiment.

[0045] Example 4: Experimental study on the sterilization effect of Staphylococcus aureus by low-temperature plasma fusion with 222nm ultraviolet light with different numbers of electrode network layers

[0046] Pipette 10 μL of 10 8 CFU / mL Staphylococcus aureus (Gram-positive bacteria) suspension was dropped onto a 1 cm × 1 cm quartz glass slide (after degreasing and disinfection) and evenly spread over a 1 cm 2 The dried glass slides were then subjected to different treatments (the sample was 9 ± 0.5 cm from the plasma airflow outlet and 4.3 ± 0.2 cm from the centerline of the UV light source): ① low-temperature plasma (30 s) followed by 222 nm UV (30 s), ② 222 nm UV (30 s) followed by low-temperature plasma (30 s), ③ low-temperature plasma (60 s) followed by 222 nm UV (60 s), ④ 222 nm UV (60 s) followed by low-temperature plasma (60 s), ⑤ low-temperature plasma fusion with 222 nm UV (30 s), and ⑥ low-temperature plasma fusion with 222 nm UV (60 s). These treatments are labeled CP+UV-1, UV+CP-1, CP+UV-2, UV+CP-2, UV-CP-1 integration, and UV-CP-2 integration, respectively. The untreated group served as the control group. The treated groups had one, two, and three electrode network layers, respectively. Treatment parameters: The operating voltage of the low-temperature plasma gas flow generator is 33±5kV, the high-voltage electric field operating frequency is 8.5kHz, and the low-temperature plasma power density is 2.5W / cm 2 The wavelength of the UV generator is 222nm, and the UV power density is 0.6W / cm 2 The electrode grids were arranged in parallel. The antibacterial effect was determined by the plate count method.

[0047] Table 4 Effects of different electrode network layers on Staphylococcus aureus

[0048]

[0049] As can be seen from Table 4, the number of Staphylococcus aureus in the control group was 6.50±0.19Log 10 CFU / cm 2 After different treatments, in the same group: the UV-CP-1 integrated group was always lower than the CP+UV-1 and UV+CP-1 groups; the UV-CP-2 integrated group was always lower than the CP+UV-2 and UV+CP-2 groups, indicating that the sterilization effect of the low-temperature plasma fusion 222nm ultraviolet integrated treatment was significantly higher than that of the sequential treatment groups; in different groups: the number of Staphylococcus aureus in the 1st, 2nd and 3rd layers of UV+CP were 2.45±0.09, 1.65±0.13 and 2.08±0.17Log respectively 10 CFU / cm 2 , killing 4.05, 4.85, and 4.42 Log values, respectively, and there were significant differences between the different electrode mesh layers (p < 0.05). Therefore, the preferred number of electrode mesh layers is 2 for the following experiments.

[0050] Example 5: Experimental study on the sterilization effect of Staphylococcus aureus by low-temperature plasma fusion with 222nm ultraviolet light using different electrode and tube network arrangements

[0051] Pipette 10 μL of 10 8 CFU / mL Staphylococcus aureus (Gram-positive bacteria) suspension was dropped onto a 1 cm × 1 cm quartz glass slide (after degreasing and disinfection) and evenly spread over a 1 cm 2 The dried glass slides were then subjected to different treatments (the sample was 9 ± 0.5 cm from the plasma airflow outlet and 4.3 ± 0.2 cm from the centerline of the UV light source): ① low-temperature plasma (30 s) followed by 222 nm UV (30 s), ② 222 nm UV (30 s) followed by low-temperature plasma (30 s), ③ low-temperature plasma (60 s) followed by 222 nm UV (60 s), ④ 222 nm UV (60 s) followed by low-temperature plasma (60 s), ⑤ low-temperature plasma fusion with 222 nm UV (30 s), and ⑥ low-temperature plasma fusion with 222 nm UV (60 s). These treatments are labeled CP+UV-1, UV+CP-1, CP+UV-2, UV+CP-2, UV-CP-1 integration, and UV-CP-2 integration, respectively. The untreated group served as the control group. The treated groups had the electrode network arranged in a cross pattern and in a parallel pattern, respectively. Treatment parameters: The operating voltage of the low-temperature plasma gas flow generator is 33±5kV, the high-voltage electric field operating frequency is 8.5kHz, and the low-temperature plasma power density is 2.5W / cm 2 The wavelength of the UV generator is 222nm, and the UV power density is 0.6W / cm 2The low-temperature plasma airflow rate in the air circulation system is 4-6m 3 / min. 2 layers of ceramic electrode mesh. Antibacterial efficacy was determined by plate count method.

[0052] Table 5 Effects of different electrode network arrangements on Staphylococcus aureus

[0053]

[0054] As can be seen from Table 5, the number of Staphylococcus aureus in the control group was 6.83±0.24Log 10 CFU / cm 2 After different treatments, in the same group: the UV-CP-1 integrated group was always lower than the CP+UV-1 and UV+CP-1 groups; the UV-CP-2 integrated group was always lower than the CP+UV-2 and UV+CP-2 groups, indicating that the sterilization effect of the low-temperature plasma fusion 222nm ultraviolet integrated treatment was significantly higher than that of the sequential treatment groups; in different groups: the number of Staphylococcus aureus in the cross and parallel UV+CP treatments were 1.32±0.16 and 1.83±0.15Log respectively 10 CFU / cm 2 , killing 5.51 and 5.00 Log values, respectively. There were significant differences between the different electrode network arrangements (p < 0.05). Therefore, the cross-arrangement of the electrode network was preferred for the following experiments.

[0055] Example 6: Experimental study on the killing effect of low-temperature plasma fusion with 222nm ultraviolet light at different treatment times on Staphylococcus aureus

[0056] Pipette 10 μL of 10 8 CFU / mL Staphylococcus aureus (Gram-positive bacteria) suspension was dropped onto a 1 cm × 1 cm quartz glass slide (after degreasing and disinfection) and evenly spread over a 1 cm 2 , dried at room temperature. The dried glass pieces were placed in a culture dish and subjected to low-temperature plasma fusion 222nm ultraviolet treatment for different treatment times (the sample was 9±0.5cm away from the plasma airflow outlet surface, and 4.3±0.2cm high from the center line plane of the ultraviolet light source). The untreated group served as the control group, and the treated group: the treatment times were 20, 40, 60, 80, 100, 120, 140 and 160s, respectively. Treatment parameters: the operating voltage of the low-temperature plasma airflow generator was 33±5kV, the operating frequency of the high-voltage electric field was 8.5kHz, the low-temperature plasma power density was 2.5W / cm2, and the ultraviolet power density was 0.6W / cm 2The ceramic electrode mesh was arranged in parallel with two layers. The antibacterial effect was determined by plate count. A temperature measuring instrument was used to measure the surface temperature changes of the samples subjected to low-temperature plasma fusion and 222nm ultraviolet light at different treatment times.

[0057] Table 6 Effects of different treatment times on Staphylococcus aureus

[0058] Processing time (s) Staphylococcus aureus count (Log10 CFU / cm2) Sterilization rate (%) Temperature (℃) control 6.47±0.06 - 22.4 20 5.69±0.07 80.7478 24.5 40 3.54±0.26 99.8643 25.5 60 1.93±0.06 99.9967 27.1 80 1.83±0.07 99.9973 28.7 100 1.50±0.15 99.9988 29.4 120 1.26±0.06 99.9993 30.6 140 0.79±0.33 99.9998 31.5 160 0 100.0000 32.5

[0059] As can be seen from Table 6, the number of Staphylococcus aureus in the control group was 6.47±0.06Log 10 CFU / cm 2 , after low-temperature plasma fusion 222nm UV treatment, the number of Staphylococcus aureus at treatment times of 20, 40, 60, 80, 100, 120 and 140s were: 5.69±0.06, 3.54±0.26, 1.93±0.06, 1.83±0.07, 1.50±0.15, 1.26±0.06 and 0.79±0.33Log, respectively. 10 CFU / cm 2 , killing 0.78, 2.93, 4.54, 4.63, 4.97, 5.20, and 5.68 Log values, respectively. When the treatment time was 160 seconds, all Staphylococcus aureus were killed. As the treatment time increased, the sterilization effect improved. As the treatment time increased, the sample surface temperature showed a downward-increasing trend, remaining below 34°C.

[0060] Example 7: Experiment on the disinfection of novel coronavirus (SARS-CoV-2) by low-temperature plasma fusion with 222nm ultraviolet

[0061] According to the test optimization results of the above 6 examples with Staphylococcus aureus as the disinfection target, and the example with the new coronavirus (SARS-CoV-2) as the targeted disinfection target, a disinfection effect test was carried out.

[0062] At 20℃ ambient temperature and 50% relative humidity, 10 μL of 10 7 A suspension of coronavirus (SARS-CoV-2: BetaCoV / JS02 / Human / 2019) with a TCID 50 / mL was dropped onto a 1 cm × 1 cm quartz glass slide (after degreasing and disinfection) and evenly spread over a 1 cm 2, and let it stand to dry. The dried glass slides were placed in a culture dish and subjected to low-temperature plasma fusion 222nm ultraviolet treatment for different treatment times (the sample was 9±0.5cm away from the plasma airflow outlet surface and 4.3±0.2cm away from the center line plane of the ultraviolet light source). The untreated group was the positive control group (virus control group) and the negative control group (cell control group). The treatment groups: the treatment times were 20, 40, 60, 80, 100, 120, 140 and 160s respectively. Treatment parameters: the operating voltage of the low-temperature plasma airflow generator was 33±5kV, the high-voltage electric field operating frequency was 8.5kHz, the low-temperature plasma power density was 2.5W / cm2, the wavelength of the ultraviolet generator was 222nm, the ultraviolet power density was 0.6W / cm2, the ceramic electrode mesh was 2 layers, and the electrode meshes were arranged in parallel. The infectivity of the coronavirus (SARS-CoV-2) was determined by the TCID 50 virus titer determination method. (The experimental materials were provided by the Jiangsu Provincial Center for Disease Control and Prevention, and the experiments were conducted in the P3 laboratory of the Jiangsu Provincial Center for Disease Control and Prevention.) A temperature measuring instrument was used to measure the surface temperature changes of the samples subjected to 222nm ultraviolet radiation from the low-temperature plasma fusion at different treatment times. Ozone content was measured using an ozone detector. Treatment parameters: high-voltage electric field operating frequency of 8.5kHz, low-temperature plasma power density of 2.5W / cm 2 , UV power density 0.6W / cm 2 , 2 layers of ceramic electrode mesh, the electrode meshes are arranged in parallel, and the processing time is 60s.

[0063] Table 7 Ozone and temperature changes

[0064] Time(s) Ozone (ppm) Temperature (℃) 0 1100 19.1 30 1090 22.1 60 1020 24.5 90 1000 26.1 120 1112 29.3 150 1077 29.8

[0065] Table 8 Disinfection of novel coronavirus

[0066] Group <![CDATA[Coronavirus titer (log 10 TCID 50 / cm 2 )]]> Killing rate (%) Positive control group 4.28 — Negative control group 0 — 30s 0 100 60s 0 100 90s 0 100 120s 0 100 150s 0 100

[0067] As can be seen from Tables 7 and 8, after low-temperature plasma fusion 222nm ultraviolet treatment, no coronavirus was detected at treatment times of 30, 60, 90, 120, and 150s, and the killing rate was 100%. The cells in the negative control group grew normally, the average logarithmic value and range of the virus titer in the positive control group was 4.28 (4.00-4.50), and the cells in the disinfection test group showed no lesions, which was consistent with the cell growth of the negative control group. As the treatment time increased, the surface temperature of the sample showed a downward and upward trend. When the treatment time was 150s, the surface temperature of the sample was below 30°C, and the ozone content was maintained at around 1000-1100ppm, indicating that the disinfection effect of low-temperature plasma fusion 222nm ultraviolet on the new coronavirus (SARS-CoV-2) was not related to the temperature rise within 150 seconds, maintaining the characteristics of low-temperature plasma cold sterilization.

[0068] The test conclusion of the BSL-3 laboratory of Jiangsu Provincial Center for Disease Control and Prevention in accordance with the national health industry standard "Laboratory Evaluation Standard for Coronavirus Disinfection Effect" (WS / T 775-2021) is as follows: Under the conditions of 20°C test environment temperature and 50% relative humidity, the low-temperature plasma fusion 222nm ultraviolet new coronavirus disinfection test equipment is 9±0.5cm away from the plasma electrode tube (plasma airflow outlet plane) and 4.3±0.2cm away from the center line plane of the ultraviolet light source. The sample disinfection time is 30 seconds, and the new coronavirus (SARS-CoV-2) stained on the glass slide is completely inactivated, and the disinfection effect is qualified.

[0069] The above description is merely a preferred embodiment of the present invention and is not intended to limit the present invention. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art will be able to modify the technical solutions described in the aforementioned embodiments or replace some of the technical features therein with equivalents. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principles of the invention shall be included within the scope of protection of the present invention.

Claims

1. A low-temperature plasma fusion ultraviolet special pathogen coronavirus disinfection technology device, characterized by: The technical device includes a low-temperature plasma airflow generator, an ultraviolet generator and an airflow circulation system; the ultraviolet generator is located in front of the low-temperature plasma airflow, and the disinfection airflow formed by the fusion of the low-temperature plasma airflow and ultraviolet light is sprayed onto the surface of the sample to be disinfected, thereby disinfecting the pathogens and coronaviruses on the sample surface; The low-temperature plasma airflow generator includes an electrode tube assembly, an electrode tube frame, a micro fan, and a fan cover frame; the electrode tube frame is provided with 2-5 layers of ceramic electrode tubes, each layer is provided with 3-15 electrode tubes, the two layers of electrode tubes are arranged crosswise or in parallel, and the vertical and horizontal spacing L of the electrode tubes is 6±2mm; the two ends of the electrode tubes are connected together outside the electrode frame and connected to the two poles of the high-voltage power supply; The ultraviolet generator consists of 2-5 groups of ultraviolet light sources, which are evenly distributed in front of the low-temperature plasma airflow plane. The height of the sample to be processed from the center line plane of the ultraviolet light source is 4-5 cm, and the distance between the sample to be processed and the plasma airflow outlet surface is 8-12 cm.

2. The low-temperature plasma fusion ultraviolet special pathogen coronavirus disinfection technology device according to claim 1 is characterized in that: The airflow circulation system includes an axial flow fan, a gas pipeline, and a flow sensor. The gas flow sensor-PLC is used to control the axial flow fan to regulate the low-temperature plasma airflow.

3. The low-temperature plasma fusion ultraviolet special pathogen coronavirus disinfection technology device according to claim 1, characterized in that: The micro fans set in the low-temperature plasma airflow generator are evenly distributed on the fan cover frame, and the distance h2 between the fan plane and the center plane of the upper electrode tube is 20±5 mm; the air flow rate of the micro fans is 1-8 m 3 / min.

4. The low-temperature plasma fusion ultraviolet special pathogen coronavirus disinfection technology device according to claim 1, characterized in that: The ceramic electrode tube consists of a ceramic tube shell, metal powder and a silicone seal, with an outer diameter of 15-25 mm and a wall thickness of 1-3 mm; the silicone seal material used at both ends is SiO2, and a copper or aluminum wire with a diameter of 2±0.5 mm passes through the center of the silicone seal.

5. The low-temperature plasma fusion ultraviolet special pathogen coronavirus disinfection technology device according to claim 4 is characterized in that: The ceramic tube is filled with metal powder, which consists of aluminum powder, magnesium powder, copper powder and titanium powder. The weight proportions of the components are 30-60 parts, 30-60 parts, 10-30 parts and 0.1-5 parts respectively. The particle size of the metal powder is 80-150 mesh.

6. A method for disinfecting pathogenic bacteria coronavirus using the technical device according to claim 1, characterized in that: The disinfection airflow formed by the fusion of low-temperature plasma airflow and ultraviolet light is sprayed onto the surface of the sample to be disinfected for 30 seconds to 150 seconds to disinfect the pathogens or coronaviruses on the sample surface; the disinfection airflow is recycled through the air circulation system to improve the disinfection efficiency and reduce O3 emissions.

7. The method according to claim 6, wherein: The operating voltage of the low-temperature plasma gas flow generator is 10-40 kV, the operating frequency is 8-20 kHz, and the low-temperature plasma power density is 0.5-2.5 W / cm 2 The UV generator produces UV light with a wavelength of 222 ± 10 nm and a power density of 0.25-0.65 W / cm 2 .

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