Microfluidic device and control method

Abstract

The present invention discloses a microfluidic device and a control method. The microfluidic device includes a microfluidic chip and a control device. The microfluidic chip is provided with a plurality of chambers and a plurality of flow channels. Each flow channel is provided with a valve hole, and all the valve holes are arranged on the same straight line; the control device includes a curve driving component and a valve hole control component. The valve control component includes a guiding seat and a plurality of control valve columns. The two ends of the control valve column are respectively opposite to the valve hole and the output end of the curve driving component; the control method includes: controlling the curve driving component to move to a preset position, and the curve driving component simultaneously controls each control valve column to realize independent lifting, so that each control valve column controls each valve hole to realize independent on-off at the same moment. By arranging all the valve holes on the same straight line, and the curve driving component can control the on-off states of different valve holes with independent control effects at the same moment, the control operation is simple and convenient.

Description

Technical Field

[0001] The present invention relates to the field of microfluidic chips, and particularly to a microfluidic device and a control method thereof. Background Art

[0002] A microfluidic chip integrates basic operation units such as sample preparation, reaction, separation, and detection in the biological, chemical, and medical analysis processes onto a chip with a micron scale, and the microfluidic chip can cooperate to complete the analysis process.

[0003] Valves for controlling the communication relationship between chambers are provided in the microfluidic chip. The valves of the microfluidic chip can be set as rotary valves, centrifugal force-driven valves, membrane valves, etc. A valve membrane refers to a soft film attached to the surface of the valve hole of a hard chip, and the film is deformed by mechanical pressing to push and block the valve hole or open and connect the valve hole, thereby controlling the flow or stop of the flow channel where the valve hole is located. In the related art, each valve hole needs to be provided with a corresponding independent pressing device, and a corresponding program needs to be set to achieve control. The control of the microfluidic chip is complex and cumbersome, and the control cost of the microfluidic chip is high. Summary of the Invention

[0004] The present invention aims to at least solve one of the technical problems existing in the prior art. For this reason, the present invention provides a microfluidic device and a control method. By arranging all the valve holes inside the microfluidic chip in the same straight line, the control device can uniformly control the on-off states of all valves, and the control operation is convenient.

[0005] The first aspect embodiment of the present invention provides a microfluidic device, including a microfluidic chip and a control device. The microfluidic chip is provided with a plurality of chambers and a plurality of flow channels. The flow channels connect corresponding two chambers, and each flow channel is provided with a valve hole. All the valve holes are arranged in the same straight line. The control device includes a curve driving component and a valve control component. The valve control component includes a guiding seat and a plurality of control valve columns. The control valve columns are slidably connected to the guiding seat. The number of valve holes is equal to that of the control valve columns. The two ends of each control valve column are respectively opposite to the valve hole and the output end of the curve driving component. The curve driving component is used to drive each control valve column to leave or enter the corresponding valve hole respectively to control the on-off of the corresponding valve hole.

[0006] According to the above embodiments of the present invention, there are at least the following beneficial effects: corresponding chambers are provided in the chip body and flow channels connecting the chambers are provided, and valve holes are provided in the corresponding flow channels and cooperate with the control valve columns to control the corresponding valve holes to block or connect the flow channels. The valves inside the microfluidic chip have a low cost. By arranging all the valve holes in the same straight line, and the curve driving component can simultaneously control each control valve column to respectively achieve independent lifting to reach the corresponding position, and further control the on-off states of different valve holes with independent control effects at the same moment. The structure is simple, the control operation is convenient, and the control cost is low.

[0007] According to some embodiments of the first aspect of the present invention, the microfluidic chip also includes an elastic film, the valve hole is a blind hole, the elastic film is arranged at the opening of the valve hole, and the control valve column is located on the side of the elastic film away from the valve hole, so that the control valve column can push the elastic film into the valve hole to achieve cutoff.

[0008] According to some embodiments of the first aspect of the present invention, the curve drive assembly includes a rotating shaft and a plurality of cams, each cam is connected to the rotating shaft, the number of cams and control valve columns is equal, one end of the control valve column away from the valve hole is opposite to the cam, and the cam is used to drive the corresponding control valve column to leave or enter the valve hole, so that when the rotating shaft rotates, each control valve column controls the opening and closing of the corresponding valve hole respectively.

[0009] According to some embodiments of the first aspect of the present invention, the guide seat includes an upper guide plate and a spring, the upper guide plate is provided with an upper guide hole, the control valve column is slidably connected in the upper guide hole, the spring is sleeved outside the control valve column, the control valve column is provided with a limit piece, one end of the spring abuts against a side of the upper guide plate close to the curve driving component, and the other end of the spring abuts against a side of the limit piece away from the curve driving component, so that the limit piece forms a movement tendency away from the microfluidic chip.

[0010] According to some embodiments of the first aspect of the present invention, the guide seat further comprises a lower guide plate, the lower guide plate is provided with a lower guide hole, and the control valve column is slidably connected in the lower guide hole.

[0011] According to some embodiments of the first aspect of the present invention, the control device also includes a support plate, which is provided with a limit groove and a plurality of clearance holes. The clearance holes are arranged at the bottom of the limit groove, and the limit groove is used to install the microfluidic chip. The control valve column is slidably connected in the corresponding clearance hole, and the support plate is located on the side of the guide seat away from the curve drive assembly.

[0012] A second aspect of the present invention provides a control method for a microfluidic device, wherein the microfluidic device comprises a microfluidic chip and a control device, wherein a plurality of chambers and a plurality of flow channels are provided in the microfluidic chip, wherein the flow channels connect two corresponding chambers, and each flow channel is provided with a valve hole, and all the valve holes are arranged on the same straight line; the control device comprises a curve driving component and a valve hole control component, wherein the valve control component comprises a guide seat and a plurality of control valve columns, wherein the control valve columns are slidably connected to the guide seat, wherein the number of valve holes and control valve columns is equal, and the two ends of the control valve column are respectively opposite to the valve hole and the output end of the curve driving component;

[0013] Control methods include:

[0014] The curve driving component is controlled to move to a preset position, and the curve driving component simultaneously controls each control valve column to realize independent lifting and lowering, so that each control valve column controls each valve hole to realize independent opening and closing at the same time.

[0015] According to the above embodiments of the present invention, there are at least the following beneficial effects: corresponding chambers are provided in the chip body and flow channels connecting the respective chambers are provided, and valve holes are provided in the corresponding flow channels and cooperate with control valve columns to control the corresponding valve holes to block or connect the flow channels. The valves inside the microfluidic chip have low costs. By arranging all the valve holes on the same straight line, and the curve driving assembly can simultaneously control the respective control valve columns to independently lift and lower to reach the corresponding positions, thereby controlling the on-off states of different valve holes with independent control effects at the same time. The structure is simple, the control operation is convenient, and the control cost is low.

[0016] According to some embodiments of the second aspect of the present invention, the curve driving assembly includes a rotating shaft and a plurality of cams. Each cam is connected to the rotating shaft, and the number of cams is equal to the number of control valve columns. One end of the control valve column away from the valve hole faces the cam; controlling the curve driving assembly to move to a preset position, the curve driving assembly simultaneously controls the respective control valve columns to independently lift and lower, so that each control valve column controls the corresponding valve hole to be independently opened and closed at the same time, including:

[0017] Controlling the rotating shaft and the cams to synchronously rotate to a preset angle, and each cam respectively drives the corresponding control valve column to independently lift and lower, so that each control valve column controls the corresponding valve hole to be independently opened and closed at the same time.

[0018] According to some embodiments of the second aspect of the present invention, there are a plurality of preset angles, and the position combination states of the corresponding cams at each preset angle are different, so that the on-off combination states of the corresponding valve holes at different preset angles are different.

[0019] According to some embodiments of the second aspect of the present invention, controlling the rotating shaft and the cams to synchronously rotate to a preset angle, and each cam respectively drives the corresponding control valve column to independently lift and lower, so that the control valve column controls the on-off of the corresponding valve hole, including:

[0020] Controlling the rotating shaft to rotate to the initial preset angle, and each cam respectively drives the corresponding control valve column to independently lift and lower, so that the control valve column controls the on-off of the corresponding valve hole;

[0021] Controlling the rotating shaft to rotate to the next preset angle and controlling the on-off of the corresponding valve hole until all the preset angles are traversed and the control ends.

[0022] The additional aspects and advantages of the present invention will be partially given in the following description, partially become obvious from the following description, or be understood through the practice of the present invention. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] The above and / or additional aspects and advantages of the present invention will become obvious and easy to understand from the description of the embodiments in conjunction with the following drawings, where:

[0024] Figure 1 It is a schematic structural diagram of a microfluidic chip in the microfluidic device according to an embodiment of the present invention;

[0025] Figure 2 It is along Figure 1 The schematic cross-sectional structure diagram taken along A-A in;

[0026] Figure 3 It is a schematic structural diagram of the microfluidic device according to an embodiment of the present invention;

[0027] Figure 4 It is a schematic step diagram of the control method of the microfluidic device according to an embodiment of the present invention;

[0028] Figure 5 It is Figure 3 The specific step schematic diagram of S400 in;

[0029] Figure 6 It is Figure 4 The specific step schematic diagram of S410 in.

[0030] Reference numerals:

[0031] Microfluidic chip 100, elastic film 101, sample chamber 110, reagent chamber 120, reaction chamber 130, input channel 131, hybridization chamber 140, first flow channel 150, first valve hole 151, second flow channel 160, second valve hole 161, third flow channel 170, third valve hole 171, waste liquid chamber 180, fourth flow channel 190;

[0032] Curve driving assembly 200, rotating shaft 210, first cam 220, second cam 230, third cam 240;

[0033] Valve control assembly 300, guide seat 310, upper guide plate 311, upper guide hole 312, lower guide plate 313, lower guide hole 314, first control valve column 320, second control valve column 330, third control valve column 340, supporting plate 350, limiting groove 351, relief hole 352, spring 360. Detailed implementation manners

[0034] In the description of the present invention, unless otherwise clearly defined, terms such as "setting", "installation", "connection", etc. should be understood in a broad sense, and those skilled in the art can reasonably determine the specific meanings of the above terms in the present invention in combination with the specific content of the technical solution. In the description of the present invention, the meaning of "several" is one or more, the meaning of "multiple" is two or more, "greater than", "less than", "exceeding", etc. are understood as not including the number itself, and "above", "below", "within", etc. are understood as including the number itself. In addition, features defined with "first", "second" may explicitly or implicitly include one or more of such features. In the description of the present invention, unless otherwise stated, the meaning of "multiple" is two or more.

[0035] A microfluidic chip integrates basic operation units such as sample preparation, reaction, separation, and detection in the biological, chemical, and medical analysis processes onto a chip at the micron scale, and the microfluidic chip can cooperate to complete the analysis process.

[0036] The microfluidic chip is provided with valves for controlling the communication relationship between chambers, and the valves of the microfluidic chip can be set as rotary valves, centrifugal force-driven valves, membrane valves, etc. The rotary valve controls the opening and closing of the liquid passage by rotating the valve, which needs to be embedded inside the chip and has a high manufacturing difficulty; the centrifugal force-driven valve is a valve that relies on the gradient centrifugal force to enable the liquid to break through, but it requires a corresponding centrifuge machine, and the valve control cost is high. The valve membrane refers to a soft film attached to the surface of the valve hole of the hard chip, and the film is deformed by mechanical pressing to push and block the valve hole or open and connect the valve hole, thereby controlling the flow or stop of the flow channel where the valve hole is located. Although the setting cost of the membrane valve is relatively low, in actual use, each valve hole needs to be provided with a corresponding independent pressing device, and a corresponding program needs to be set to control the corresponding blocking or connection, and the control of the microfluidic chip is complex and cumbersome, and the control cost of the microfluidic chip is high.

[0037] The following refers to Figures 1 to 6 Describe a microfluidic device and control method of the present invention, which can achieve a fast and reliable control effect on the microfluidic chip. By setting all the valve holes inside the microfluidic chip to be on the same straight line, the control device can uniformly control the on-off states of all valves, and the control operation is convenient.

[0038] Refer to Figures 1 to 3As shown in the figure, an embodiment of the first aspect of the present invention describes a microfluidic device, which includes a microfluidic chip 100 and a control device. The microfluidic chip 100 is provided with a plurality of chambers and a plurality of flow channels. The flow channels connect two corresponding chambers. Each flow channel is provided with a valve hole, and all the valve holes are arranged on the same straight line. The control device includes a curve driving component and a valve control component 300. The valve control component 300 includes a guiding seat 310 and a plurality of control valve columns. The control valve columns are slidably connected to the guiding seat 310, that is, the control valve columns can reciprocate along the connection direction of the cam and the valve hole under the restriction of the guiding seat 310. The number of valve holes is equal to the number of control valve columns. The upper and lower ends of the control valve columns are respectively opposite to the valve hole and the output end of the curve driving component. All the control valve columns are located in the same plane. The curve driving component is used to output different strokes at the same time for different positions. Furthermore, the curve driving component drives the corresponding control valve columns to leave or enter the corresponding valve holes respectively, so that each control valve column controls the on-off of the corresponding valve hole when the curve driving component drives.

[0039] Corresponding chambers are arranged in the chip body and flow channels connecting the chambers are arranged. And valve holes are arranged in the corresponding flow channels and cooperate with the control valve columns to control the blocking or connecting of the corresponding valve holes of the flow channels. The valve cost inside the microfluidic chip 100 is low. By arranging all the valve holes on the same straight line and enabling the curve driving component to simultaneously control the independent lifting of each control valve column to reach the corresponding position respectively, the on-off states of different valve holes can be controlled with independent control effects at the same moment. The structure is simple, the control operation is convenient, and the control cost is low.

[0040] It can be understood that the microfluidic chip 100 further includes an elastic film. The valve hole is a blind hole. The elastic film is arranged at the opening of the valve hole. The control valve column is located on the side of the elastic film away from the valve hole, so that the control valve column can push the elastic film into the valve hole to achieve truncation. When the control valve hole is truncated, the control valve column pushes the elastic film to squeeze into the valve hole. After the elastic film is pushed to abut against the inner wall of the valve hole, the valve hole is blocked in a highly sealed manner. When the control valve hole is connected, the control valve column moves away from the valve hole and vacates the elastic film, and the valve hole is connected.

[0041] It can be understood that the curve driving component includes a rotating shaft 210 and a plurality of cams. Each cam is connected to the rotating shaft 210. The number of cams is equal to the number of control valve columns. The end of the control valve column away from the valve hole is opposite to the cam. The cam is used to drive the corresponding control valve column to leave or enter the valve hole, so that each control valve column controls the on-off of the corresponding valve hole when the rotating shaft 210 rotates. The control valve columns are arranged in sequence along the direction parallel to the rotating shaft 210, that is, all the control valve columns are located in the same plane.

[0042] It should be noted that the curve driving component can also be other structures that can achieve different lifting and positioning effects at different positions simultaneously, such as a crank-slider mechanism, or a push bar with a driving curved surface on its surface, etc.

[0043] It can be understood that the guiding seat includes an upper guiding plate 311 and a spring 360. The upper guiding plate 311 is provided with an upper guiding hole 312. The control valve column is slidably connected in the upper guiding hole 312. The spring 360 is sleeved outside the control valve column. The control valve column is provided with a limiting member. One end of the spring 360 abuts against one side of the upper guiding plate close to the curve driving component 200, and the other end of the spring 360 abuts against one side of the limiting member far from the curve driving component 200, so that the limiting member forms a movement trend away from the microfluidic chip 100.

[0044] It can be understood that the guiding seat 310 further includes a lower guiding plate 313. The lower guiding plate 313 is provided with a lower guiding hole 314. The control valve column is slidably connected in the lower guiding hole 314.

[0045] It can be understood that there are four chambers, which are a sample chamber 110, a reagent chamber 120, a reaction chamber 130, and a hybridization chamber 140 respectively. There are three flow channels, which are a first flow channel 150, a second flow channel 160, and a third flow channel 170 respectively. The first flow channel 150 is connected between the sample chamber 110 and the reaction chamber 130. The second flow channel 160 is connected between the reagent chamber 120 and the reaction chamber 130. The third flow channel 170 is connected between the reaction chamber 130 and the hybridization chamber 140. A first valve hole 151 is provided in the first flow channel 150, a second valve hole 161 is provided in the second flow channel 160, and a third valve hole 171 is provided in the third flow channel 170. The center points of the first valve hole 151, the second valve hole 161, and the third valve hole 171 are arranged on the same straight line, which is convenient for control design; the elastic film 101 covers the ports of the first valve hole 151, the second valve hole 161, and the third valve hole 171. The elastic film 101 is used to block at least one of the first valve hole 151, the second valve hole 161, and the third valve hole 171.

[0046] It can be understood that there are three cams, namely the first cam 220, the second cam 230, and the third cam 240. The first cam 220, the second cam 230, and the third cam 240 are all connected to the rotating shaft 210. The first cam 220, the second cam 230, and the third cam 240 rotate synchronously following the rotating shaft 210. By setting the relationship among the first cam 220, the second cam 230, and the third cam 240, different driving effects can be simultaneously controlled. There are three control valve columns, namely the first control valve column 320, the second control valve column 330, and the third control valve column 340. The center points of the first valve hole 151, the second valve hole 161, and the third valve hole 171 are located on the same straight line, and this straight line is within the plane where the first control valve column 320, the second control valve column 330, and the third control valve column 340 are located. The first control valve column 320, the second control valve column 330, and the third control valve column 340 are all slidably connected to the guide seat 310. The guide seat 310 is used to limit the movement directions of the first control valve column 320, the second control valve column 330, and the third control valve column 340. One end of the first control valve column 320 is located on the circumferential surface of the first cam 220, and the other end of the first control valve column 320 is used to push the elastic film 101 to control the on / off of the first valve hole 151. One end of the second control valve column 330 is located on the circumferential surface of the second cam 230, and the other end of the second control valve column 330 is used to push the elastic film 101 to control the on / off of the second valve hole 161. One end of the third control valve column 340 is located on the circumferential surface of the third cam 240, and the other end of the third control valve column 340 is used to push the elastic film 101 to control the on / off of the third valve hole 171.

[0047] It can be understood that the first cam 220, the second cam 230, and the third cam 240 are clamped to the rotating shaft 210. The rotating shaft 210 is parallel to the straight line where the first valve hole 151, the second valve hole 161, and the third valve hole 171 are located. The rotating shaft 210 is driven to rotate by a servo motor. The first control valve column 320, the second control valve column 330, and the third control valve column 340 are respectively located directly below the first valve hole 151, the second valve hole 161, and the third valve hole 171. The first cam 220, the second cam 230, and the third cam 240 are respectively located directly below the first control valve column 320, the second control valve column 330, and the third control valve column 340. In addition to being clamped to the rotating shaft 210, the first cam 220, the second cam 230, and the third cam 240 can also be connected by means such as welding and gluing.

[0048] It can be understood that the first valve hole 151, the second valve hole 161 and the third valve hole 171 are all blind holes, and the elastic film 101 is arranged at the openings of the first valve hole 151, the second valve hole 161 and the third valve hole 171. The elastic film 101 is pushed into the first valve hole 151, the second valve hole 161 and the third valve hole 171 respectively through the first control valve column 320, the second control valve column 330 and the third control valve column 340, so as to form an effect of blocking the corresponding flow channel. After loosening the elastic film 101, the corresponding valve hole can form an effect of connecting the corresponding flow channel.

[0049] It is understandable that the microfluidic chip 100 further includes an input channel 131, the reaction chamber 130 is connected to the input channel 131, the input channel 131 is used to connect to an external input device, and the airflow driving device is used to form positive air pressure or negative air pressure to drive the gas flow inside the microfluidic chip 100. Among them, the input device can be a driving airflow driving device, a material input device, etc., which can provide corresponding driving force for the chambers and flow channels inside the microfluidic chip 100 to achieve controlled flow of liquid between chambers.

[0050] It is understandable that the microfluidic chip 100 further includes a waste liquid chamber 180 and a fourth flow channel 190 . The fourth flow channel 190 connects the hybridization chamber 140 and the waste liquid chamber 180 . The waste liquid chamber 180 is used to receive waste liquid generated after hybridization of the internal chambers and flow channels of the microfluidic chip 100 .

[0051] It can be understood that the guide seat 310 includes an upper guide plate 311 and three springs 360. The upper guide plate 311 is provided with three upper guide holes 312. The first control valve column 320, the second control valve column 330 and the third control valve column 340 are respectively slidably connected to the three upper guide holes 312. The three upper guide holes 312 are used to cooperate with the clearance hole 352 to limit the positions of the first control valve column 320, the second control valve column 330 and the third control valve column 340 to ensure that the movement trajectories of the three control valve columns are controllable. The three springs 360 are respectively sleeved on the first control valve column 320, the second control valve column 330 and the third control valve column 340. A control valve column 320, a second control valve column 330 and a third control valve column 340, the first control valve column 320, the second control valve column 330 and the third control valve column 340 are all provided with a limit piece, one end of the spring 360 abuts against the side of the upper guide plate 311 close to the curve drive component 200, and the other end of the spring 360 abuts against the side of the limit piece away from the curve drive component 200, so that the limit piece forms a movement trend away from the supporting plate 350, which can ensure that the blocking or connection action of each control valve column on each valve hole is reliable.

[0052] Specifically, the guide seat 310 also includes a lower guide plate 313, which is provided with three lower guide holes 314. The first control valve column 320, the second control valve column 330 and the third control valve column 340 are respectively slidably connected to the three lower guide holes 314. The lower guide holes 314 are used to cooperate with the upper guide holes 312 and the yield holes 352 to limit the positions of the first control valve column 320, the second control valve column 330 and the third control valve column 340, ensuring that the movement trajectories of the three control valve columns are controllable, which can further improve the reliability of the valve hole blocking or connection action.

[0053] It is understandable that the microfluidic device further comprises a support plate 350, in which a limiting groove 351 and three clearance holes 352 are provided, the clearance holes 352 are provided at the bottom of the limiting groove 351, and the clearance holes 352 penetrate the bottom of the limiting groove 351 and the bottom surface of the support plate 350, the limiting groove 351 is used to install the microfluidic chip 100, the first control valve column 320, the second control valve column 330 and the third control valve column 340 are respectively slidably connected to the three clearance holes 352, and the support plate 350 is located on the side of the guide seat 310 away from the curve driving component 200. After the microfluidic chip 100 is placed in the limiting groove 351, the three clearance holes 352 are respectively connected to the first valve hole 151, the second valve hole 161 and the third valve hole 171.

[0054] The common microfluidic chips 100 in the related art are mostly controlled by pneumatic drive or multi-motor joint drive. Pneumatic drive for drug extraction establishes a complex air path, which is not only not conducive to miniaturized design, but also increases the design cost; multi-motor joint drive requires the use of multiple motors, which has high device cost, and these motors need to be jointly controlled, which has high control cost. In addition, due to the size of the motor, a larger interval needs to be set between adjacent valve holes in the microfluidic control chip, which is not conducive to the miniaturized design of the microfluidic chip 100.

[0055] The second aspect of the present invention provides a control method for a microfluidic device, wherein the microfluidic device includes a microfluidic chip 100 and a control device. The microfluidic chip 100 is provided with a plurality of chambers and a plurality of flow channels, wherein the flow channels connect two corresponding chambers, and each flow channel is provided with a valve hole, and all the valve holes are arranged on the same straight line; the control device includes a curve driving component and a valve control component 300, wherein the valve control component 300 includes a guide seat 310 and a plurality of control valve columns, wherein the control valve columns are slidably connected to the guide seat 310, that is, the control valve columns can realize reciprocating motion under the restriction of the guide seat 310, and the number of valve holes is equal to the number of control valve columns, and the two ends of the control valve columns are respectively opposite to the valve holes and the output end of the curve driving component, and the curve driving component is used to output different strokes for different positions at the same time;

[0056] refer to Figure 4 As shown, the control method includes but is not limited to the following steps:

[0057] S400: Control the curve driving component 200 to move to a preset position. The curve driving component 200 simultaneously controls each control valve stem to achieve independent lifting and lowering, so that each control valve stem controls the corresponding valve hole to achieve independent on-off at the same moment.

[0058] Corresponding chambers are provided in the chip body and flow channels connecting the chambers are provided. Valve holes are provided in the corresponding flow channels and cooperate with the control valve stems to control the corresponding valve holes to block or connect the flow channels. The valves inside the microfluidic chip 100 have low costs. By arranging all the valve holes on the same straight line and enabling the curve driving component to simultaneously control each control valve stem to independently lift and lower to reach the corresponding position, the on-off states of different valve holes can be controlled with independent control effects at the same moment. The structure is simple, the control operation is convenient, and the control cost is low.

[0059] It can be understood that the curve driving component 200 includes a rotating shaft and a plurality of cams. Each cam is connected to the rotating shaft 210. The number of cams is equal to the number of control valve stems. One end of the control valve stem away from the valve hole faces the cam.

[0060] Refer to Figure 5 As shown, in step S400, control the curve driving component 200 to move to a preset position. The curve driving component 200 simultaneously controls each control valve stem to achieve independent lifting and lowering, so that each control valve stem controls the corresponding valve hole to achieve independent on-off at the same moment, including but not limited to the following steps:

[0061] S410: Control the rotating shaft 210 and the cams to synchronously rotate to a preset angle. Each cam drives the corresponding control valve stem to achieve independent lifting and lowering, so that each control valve stem controls the corresponding valve hole to achieve independent on-off at the same moment.

[0062] It can be understood that there are multiple preset angles. The position combination states of all the cams corresponding to each preset angle are different, so that the lifting and lowering combination states of each control valve stem at different preset angles are different, and thus the on-off combination states of the corresponding valve holes at different preset angles are different. It can control different valve holes to achieve independent on-off control effects at the same moment, and the control operation is convenient. Set the corresponding number of preset angles according to different requirements, and set the rotation positions of the cams at the corresponding preset angles, so as to adjust the pushing positions of the control valve stems and finally control the on-off effects of the corresponding valve holes.

[0063] It can be understood that, refer to Figure 6 As shown, in step S410, control the rotating shaft 210 and the cams to synchronously rotate to a preset angle. Each cam drives the corresponding control valve stem to achieve independent lifting and lowering, so that the control valve stem controls the on-off of the corresponding valve hole, including but not limited to the following steps:

[0064] S411: Control the rotation shaft 210 to rotate to the initial preset angle, and each cam drives the corresponding control valve stem to achieve independent lifting respectively, so that the control valve stem controls the on-off of the corresponding valve hole;

[0065] S412: Control the rotation shaft 210 to rotate to the next preset angle, and control the on-off of the corresponding valve hole until all preset angles are traversed, and the control ends.

[0066] When the control ends, that is, the rotation shaft 210 rotates according to the rotation of all preset angles to complete the control of the preset process.

[0067] It can be understood that there are four chambers, which are the sample chamber 110, the reagent chamber 120, the reaction chamber 130 and the hybridization chamber 140 respectively. There are three flow channels, which are the first flow channel 150, the second flow channel 160 and the third flow channel 170 respectively. The first flow channel 150 is connected between the sample chamber 110 and the reaction chamber 130. The second flow channel 160 is connected between the reagent chamber 120 and the reaction chamber 130. The third flow channel 170 is connected between the reaction chamber 130 and the hybridization chamber 140. The first valve hole 151 is provided in the first flow channel 150. The second valve hole 161 is provided in the second flow channel 160. The third valve hole 171 is provided in the third flow channel 170. The first valve hole 151, the second valve hole 161 and the third valve hole 171 are arranged in the same straight line. There are three cams, which are the first cam 220, the second cam 230 and the third cam 240 respectively. The first cam 220, the second cam 230 and the third cam 240 are all connected to the rotation shaft 210. There are three control valve stems, which are the first control valve stem 320, the second control valve stem 330 and the third control valve stem 340 respectively. The first control valve stem 320, the second control valve stem 330 and the third control valve stem 340 are all slidably connected to the guide seat 310. One end of the first control valve stem 320 is located on the circumferential surface of the first cam 220, and the other end of the first control valve stem 320 is used to control the on-off of the first valve hole 151. One end of the second control valve stem 330 is located on the circumferential surface of the second cam 230, and the other end of the second control valve stem 330 is used to control the on-off of the second valve hole 161. One end of the third control valve stem 340 is located on the circumferential surface of the third cam 240, and the other end of the third control valve stem 340 is used to control the on-off of the third valve hole 171;

[0068] Control the rotation shaft 210 and the cams to rotate synchronously to the preset angle, and each cam drives the corresponding control valve stem to achieve independent lifting respectively, so that the control valve stem controls the on-off of the corresponding valve hole, including but not limited to the following steps:

[0069] The rotation shaft 210 is controlled to rotate to a first preset angle. The first cam 220 controls the first control valve stem 320 to descend to connect the first valve hole 151. The second cam 230 and the third cam 240 respectively control the second control valve stem 330 and the third control valve stem 340 to ascend to block the second valve hole 161 and the third valve hole 171. The material in the sample chamber 110 enters the reaction chamber 130 through the first flow channel 150;

[0070] The rotation shaft 210 is controlled to rotate to a second preset angle. The second cam 230 controls the second control valve stem 330 to descend to connect the second valve hole 161. The first cam 220 and the third cam 240 respectively control the first control valve stem 320 and the third control valve stem 340 to ascend to block the first valve hole 151 and the third valve hole 171. The material in the reagent chamber 120 enters the reaction chamber 130 through the second flow channel 160;

[0071] The rotation shaft 210 is controlled to rotate to a third preset angle. The third cam 240 controls the third control valve stem 340 to descend to connect the third valve hole 171. The first cam 220 and the second cam 230 respectively control the first control valve stem 320 and the second control valve stem 330 to ascend to block the first valve hole 151 and the second valve hole 161. The material in the reaction chamber 130 enters the hybridization chamber 140 through the third flow channel 170 for cultivation, and a cultivation result is obtained.

[0072] Through the above method, the corresponding microfluidic control effect can be achieved on the test liquid to sequentially perform the required mixing reaction tests.

[0073] It can be understood that an input channel 131, a waste liquid chamber 180, and a fourth flow channel 190 are further provided in the microfluidic chip 100. The fourth flow channel 190 connects the hybridization chamber 140 and the waste liquid chamber 180. The reaction chamber 130 is connected to the input channel 131, and the input channel 131 is used to connect an external input device;

[0074] After controlling the elastic film 101 to block the first valve hole 151 and the second valve hole 161 and connect the third valve hole 171, so that the material in the reaction chamber 130 enters the hybridization chamber 140 through the third flow channel 170 for cultivation and a cultivation result is obtained, the following steps are included but not limited to:

[0075] Control the input device to input a cleaning liquid through the input channel 131, so that the material in the hybridization chamber 140 enters the waste liquid chamber 180 through the fourth flow channel 190.

[0076] It should be noted that the input channel 131 is respectively connected to an independent pressure blower and a liquid supply device. The pressure blower can generate positive pressure or negative pressure, and the liquid supply device can provide a cleaning liquid.

[0077] The control method of the microfluidic device according to the second aspect embodiment will be described in detail below in combination with specific usage data:

[0078] In the initial state, the first cam 220, the second cam 230, and the third cam 240 respectively control the first control valve column 320, the second control valve column 330, and the third control valve column 340 to be in the raised position. The upwardly pushed elastic film 101 blocks the first valve hole 151, the second valve hole 161, and the third valve hole 171, and the sample chamber 110 is heated at 95 °C for 2 minutes;

[0079] The rotating shaft 210 is controlled to rotate to a first preset angle. The first cam 220 controls the first control valve column 320 to descend to connect the first valve hole 151. The second cam 230 and the third cam 240 respectively control the second control valve column 330 and the third control valve column 340 to rise, so that the elastic film 101 blocks the second valve hole 161 and the third valve hole 171. The pressure blower applies a negative pressure to the reaction chamber 130 through the input channel 131, and the material in the sample chamber 110 enters the reaction chamber 130 through the first flow channel 150;

[0080] The rotating shaft 210 is controlled to rotate to a second preset angle. The second cam 230 controls the second control valve column 330 to descend to connect the second valve hole 161. The first cam 220 and the third cam 240 respectively control the first control valve column 320 and the third control valve column 340 to rise, so that the elastic film 101 blocks the first valve hole 151 and the third valve hole 171. The pressure blower applies a negative pressure to the reaction chamber 130 through the input channel 131, and the material in the reagent chamber 120 enters the reaction chamber 130 through the second flow channel 160;

[0081] The rotating shaft 210 is controlled to rotate to a fourth preset angle to return to the initial state. The first cam 220, the second cam 230, and the third cam 240 respectively control the first control valve column 320, the second control valve column 330, and the third control valve column 340 to be in the raised position. The upwardly pushed elastic film 101 blocks the first valve hole 151, the second valve hole 161, and the third valve hole 171, and the reaction chamber 130 is heated at 40 °C for 30 minutes;

[0082] The rotating shaft 210 is controlled to rotate to a third preset angle. The third cam 240 controls the third control valve column 340 to descend to connect the third valve hole 171. The first cam 220 and the second cam 230 respectively control the first control valve column 320 and the second control valve column 330 to rise, so that the elastic film 101 blocks the first valve hole 151 and the second valve hole 161. The pressure blower applies a positive pressure to the reaction chamber 130 through the input channel 131, and the material in the reaction chamber 130 enters the hybridization chamber 140 through the third flow channel 170 for normal temperature incubation for 1 to 2 hours;

[0083] Keep the control rotation axis 210 at the third preset angle. The elastic film 101 blocks the first valve hole 151 and the second valve hole 161 and connects the third valve hole 171. The liquid supply device inputs the cleaning liquid into the reaction chamber 130 and the hybridization chamber 140 through the input channel 131. The waste liquid after cleaning reaches the waste liquid chamber 180, and the cultivation result is obtained after photographing the gold sheet in the hybridization chamber 140.

[0084] It can be understood that the first preset angle, the second preset angle, and the third preset angle correspond to 90°, 180°, and 270° respectively, the angle of the initial position corresponds to 0°, the initial position is the zero adjustment position, and the fourth preset angle is 360°. By respectively setting the lift, return stroke, near dwell angle, and far dwell angle of each cam, the rotation positions corresponding to the first cam 220, the second cam 230, and the third cam 240 at different preset angles are designed, and then the corresponding pushing or retracting effects of the first control valve stem 320, the second control valve stem 330, and the third control valve stem 340 are controlled, so that the flow states of each valve hole can be controlled simultaneously, which is convenient to control.

[0085] In the description of this specification, the description with reference to terms such as "one embodiment", "some embodiments", "schematic embodiments", "examples", "specific examples", or "some examples" means that the specific features, structures, materials, or characteristics described in connection with the embodiment or example are included in at least one embodiment or example of the present invention. In this specification, the schematic descriptions of the above terms do not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials, or characteristics described can be combined in a suitable manner in any one or more embodiments or examples.

[0086] Although the embodiments of the present invention have been shown and described, those of ordinary skill in the art can understand that various changes, modifications, substitutions, and variations can be made to these embodiments without departing from the principles and purposes of the present invention. The scope of the present invention is defined by the claims and their equivalents.

Claims

1. A microfluidic device, characterized in that, include: A microfluidic chip, wherein the microfluidic chip is provided with a plurality of chambers and a plurality of flow channels, wherein the flow channels connect two corresponding chambers, each of the flow channels is provided with a valve hole, and all the valve holes are arranged on the same straight line; A control device, comprising a curve driving component and a valve control component, wherein the valve control component comprises a guide seat and a plurality of control valve posts, wherein the control valve posts are slidably connected to the guide seat, wherein the number of the valve holes and the control valve posts are equal, wherein the two ends of the control valve posts are respectively opposite to the valve holes and the output end of the curve driving component, and wherein the curve driving component is used to drive each of the control valve posts to leave or enter the corresponding valve hole respectively, so as to control the on-off of the corresponding valve hole; The microfluidic chip further comprises an elastic film, the valve hole is a blind hole, the elastic film is arranged at the opening of the valve hole, and the control valve column is located on a side of the elastic film away from the valve hole, so that the control valve column can push the elastic film into the valve hole to achieve cutoff; The curve driving assembly includes a rotating shaft and a plurality of cams, each of which is connected to the rotating shaft, and the number of the cams and the control valve posts is equal. One end of the control valve post away from the valve hole faces the cam, and the cam is used to drive the corresponding control valve post to leave or enter the valve hole, so that when the rotating shaft rotates, each control valve post controls the opening and closing of the corresponding valve hole respectively; The guide seat comprises an upper guide plate and a spring, the upper guide plate is provided with an upper guide hole, the control valve column is slidably connected to the upper guide hole, the spring is sleeved outside the control valve column, the control valve column is provided with a limiter, one end of the spring abuts against a side of the upper guide plate close to the curve driving component, and the other end of the spring abuts against a side of the limiter away from the curve driving component, so that the limiter forms a movement trend away from the microfluidic chip; The guide seat further comprises a lower guide plate, the lower guide plate is provided with a lower guide hole, and the control valve column is slidably connected to the lower guide hole; The control device also includes a support plate, which is provided with a limit groove and a plurality of clearance holes. The clearance holes are arranged at the bottom of the limit groove, and the limit groove is used to install the microfluidic chip. The control valve column is slidably connected to the corresponding clearance hole. The support plate is located on the side of the guide seat away from the curve driving component.

2. A control method for a microfluidic device according to claim 1, characterized in that: The control method comprises: The curve driving assembly is controlled to move to a preset position, and the curve driving assembly simultaneously controls each of the control valve posts to realize independent lifting and lowering, so that each of the control valve posts can respectively control each of the valve holes to realize independent opening and closing at the same time.

3. The control method of the microfluidic device according to claim 2, wherein, The controlling of the curve driving assembly to move to a preset position, the curve driving assembly simultaneously controlling each of the control valve posts to realize independent lifting and lowering, so that each of the control valve posts respectively controls each of the valve holes to realize independent opening and closing at the same time, comprises: Control the synchronous rotation of the rotating shaft and the cam to a preset angle, and each of the cams drives the corresponding control valve stem to achieve independent lifting, so that each of the control valve stems controls the corresponding valve hole to achieve independent on-off at the same moment.

4. The control method of the microfluidic device according to claim 3, characterized in that, There are multiple preset angles, and the position combination states of the corresponding cams at each preset angle are different, so that the on-off combination states of the corresponding valve holes at different preset angles are different.

5. The control method of the microfluidic device according to claim 4, wherein, The control of the synchronous rotation of the rotating shaft and the cam to a preset angle, and each of the cams drives the corresponding control valve stem to achieve independent lifting, so that each of the control valve stems controls the corresponding valve hole to achieve independent on-off at the same moment, includes: Control the rotating shaft to rotate to the initial preset angle, and each of the cams drives the corresponding control valve stem to achieve independent lifting respectively, so that the control valve stem controls the on-off of the corresponding valve hole; Control the rotating shaft to rotate to the next preset angle and control the on-off of the corresponding valve hole until all the preset angles are traversed and the control ends.

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