An automatic feed oscillation incubator

Abstract

The application discloses an automatic material supplementing oscillation incubator, which comprises a box body, a first fixing frame, a probe, a culture bottle, an electrode sensor, a sealing flexible joint, a collection box, connecting lines and an oscillation mechanism; the first fixing frame and the collection box are fixed on the inner wall of the box body; one end of the probe is fixed on the first fixing frame through the sealing flexible joint; the probe is internally provided with the electrode sensor; one end of the electrode sensor penetrates through the sealing flexible joint and reaches into the solution in the culture bottle; the other end is connected with the probe; the electrode sensor is connected with the collection box through the probe and the connecting lines. The probe is fixed on the one side fixing frame of the first fixing chamber through the sealing flexible joint, signal interference caused by shaking of the probe and the lines is reduced, and the detection precision of the sample culture condition in the culture bottle is improved.

Description

Technical Field

[0001] This invention relates to the field of bioreactor shaking incubator technology, and in particular to an automatic feeding shaking incubator. Background Technology

[0002] Bioreactor shakers are suitable for screening and culturing various microorganisms and genetically engineered strains. High-throughput bioreactor shakers are significantly superior to conventional shakers and bioreactors. They have stable pH measurement devices and precise feeding systems, enabling further control over the culture process. At the same time, micro-feeding technology designed for shake flasks can achieve various carbon and nitrogen increases, pH control, or trace element control, which is of great significance for achieving metabolism-based research.

[0003] However, existing fed-batch shaking incubators place the feed bottles, feed pumps, etc., on a shaking plate, with the detection probe fixed to the culture bottle, all rotating with the plate. The shaking of the probe and wiring causes signal instability, making accurate monitoring impossible and affecting the culture results. Furthermore, prolonged shaking can damage the related circuitry, reducing its lifespan. Summary of the Invention

[0004] The purpose of this invention is to provide an automatic feeding and shaking incubator that can fix the probe, reduce circuit damage, and accurately detect the culture status of samples in culture flasks.

[0005] To solve the above-mentioned technical problems, the present invention provides an automatic feeding oscillating incubator, comprising: a chamber, a first fixed frame, a second fixed frame, a probe, a culture flask, an electrode sensor, a sealed flexible connector, a connecting wire, and an oscillation mechanism; the oscillation mechanism is installed inside the chamber, and the culture flask is installed on the oscillation mechanism; the first fixed frame and the junction box are fixed to the inner wall of the chamber; the sealed flexible connector is sealed and installed at the mouth of the culture flask; the probe is installed on the sealed flexible connector; one end of the probe is fixed to the first fixed frame through the sealed flexible connector; an electrode sensor is disposed inside the probe; one end of the electrode sensor passes through the sealed flexible connector and extends into the solution inside the culture flask, and the other end is connected to the probe; the electrode sensor is connected to the junction box through the probe and the connecting wire.

[0006] Furthermore, the sealing flexible joint consists of an upper cover, a flexible joint, and a lower cover; the probe is fixed on the upper cover, and the upper cover is fixed on the first fixing frame; the lower cover is sealed and installed at the mouth of the culture bottle; the flexible joint is connected between the upper cover and the lower cover.

[0007] Furthermore, the flexible connector is made of silicone.

[0008] Furthermore, the lower cover is fixed to the mouth of the culture flask by a threaded connection.

[0009] Furthermore, the automatic feeding shaking incubator also includes a second fixed frame, a feeding bottle, a feeding tube, a feeding pipe, a feeding pump, and a feeding control box; the feeding bottle and the feeding control box are placed inside the second fixed frame; a feeding pump is provided on one side of the feeding control box; the feeding pump is connected to the feeding bottle through the feeding tube; the feeding pump is connected to the culture bottle through the feeding pipe for replenishing the culture bottle with liquid.

[0010] Furthermore, the second mounting bracket is provided with an opening, through which the feed pump replenishes the culture flask with the required culture solution.

[0011] Furthermore, the material replenishment control box is connected to the hub box via the connecting cable.

[0012] Furthermore, the probe is fixed to the first mounting bracket via a threaded connection.

[0013] Furthermore, the oscillation mechanism includes an eccentric rotating shaft, a rocking plate, and a motor; the culture flask is placed on the rocking plate; the eccentric rotating shaft is connected to the rocking plate; the motor controls the eccentric rotating shaft to make the rocking plate rotate with different amplitudes.

[0014] Compared with the prior art, the present invention has at least the following beneficial effects:

[0015] This invention fixes one end of the probe and the sealed flexible connector connected to the connecting wire to a first fixing frame, which is fixed inside the box. When the oscillation mechanism drives the culture bottle to oscillate and shake, the flexible connector causes the other end of the connector to shake, but not the probe. This reduces signal interference caused by probe and wire shaking, improves the accuracy of detecting the culture status of the sample in the culture bottle, and thus ensures the culture effect. Moreover, reducing the shaking of the connecting wire helps to extend its service life.

[0016] Furthermore, the electrode sensor can monitor the pH value, dissolved oxygen and other culture medium parameters of the culture in real time. Through the monitoring and feedback of the above parameters, the feeding control box controls the feeding pump to feed the culture bottle, ensuring the continuity and stability of the culture. Attached Figure Description

[0017] Figure 1 This is a schematic diagram of the structure of an automatic feeding oscillating incubator according to one embodiment of the present invention;

[0018] Figure 2 This is a schematic diagram of the structure of a sealing flexible joint in one embodiment of the present invention;

[0019] Figure 3 This is a schematic diagram of the structure of a sealing flexible joint in one embodiment of the present invention;

[0020] Figure 4 This is a schematic diagram of the fixing mechanism in one embodiment of the present invention;

[0021] Figure 5 This is a schematic diagram of the oscillation mechanism in one embodiment of the present invention. Detailed Implementation

[0022] The following description, in conjunction with schematic diagrams, illustrates an automatic feeding oscillating incubator according to the present invention, which represents a preferred embodiment of the invention. It should be understood that those skilled in the art can modify the invention described herein while still achieving its advantageous effects. Therefore, the following description should be understood as being of general knowledge to those skilled in the art and is not intended to limit the invention.

[0023] The invention is described more specifically by way of example in the following paragraphs with reference to the accompanying drawings. The advantages and features of the invention will become clearer from the following description and claims. It should be noted that the drawings are in a very simplified form and use non-precise proportions, and are only used to facilitate and clarify the illustration of the embodiments of the invention.

[0024] This invention provides an automatic feeding and shaking incubator; please refer to [reference needed]. Figure 1 It includes: box body 1, first fixing frame 2, probe 3, culture bottle 4, electrode sensor 5, sealed flexible joint 6, connecting line 7 and oscillation mechanism 8.

[0025] Specifically, the oscillation mechanism 8 is installed inside the housing 1, and the culture flask 4 is installed on the oscillation mechanism 8; the first fixing frame 2 and the junction box 18 are fixed to the inner wall of the housing 1; the sealing flexible joint 6 is sealed and installed at the mouth of the culture flask 4; the probe 3 is installed on the sealing flexible joint 6; one end of the probe 3 is fixed to the first fixing frame 2 through the sealing flexible joint 6; an electrode sensor 5 is provided inside the probe 3; one end of the electrode sensor 5 passes through the sealing flexible joint 6 and extends into the solution inside the culture flask 4; the other end is connected to the probe 3; the electrode sensor 5 is connected to the junction box 18 through the probe 3.

[0026] In one specific embodiment, the electrode sensor 5 is a pH electrode sensor, which extends into the culture flask 4 to detect the pH value of the culture flask 4. Alternatively, the electrode sensor 5 can also be a dissolved oxygen (DO) sensor, or other desired sensors, to monitor the dissolved oxygen and other culture medium parameters within the culture flask 4.

[0027] For further details, please refer to... Figure 2 The sealing flexible joint 6 is composed of an upper cover 9, a flexible joint 10 and a lower cover 11.

[0028] Specifically, the probe 3 is fixed on the upper cover 9, which is fixed on the first fixing frame 2; the lower cover 11 is sealed and installed at the mouth of the culture bottle 4; and the flexible connector 10 is connected between the upper cover 9 and the lower cover 11.

[0029] When the oscillation mechanism 8 rotates, the culture bottle 4 rotates with the oscillation mechanism 8, and the flexible connector 10 swings back and forth. However, since the probe 3 is fixed to the first fixed frame through one end of the sealing flexible connector 6, the probe 3 no longer swings with the rotation of the culture bottle 4. At the same time, the shaking frequency of the connecting line 7 is reduced.

[0030] Specifically, the flexible connector 10 is made of silicone. Silicone has good thermal stability and is not easily deformed when the temperature inside the chamber changes, thus reducing the impact on elasticity. In addition, silicone has stable chemical properties and will not burn when the temperature inside the chamber rises. Moreover, silicone has good elasticity, so when the flexible connector 10 made of silicone is shaken back and forth, it will not collide with the mouth of the culture bottle 4, that is, it will not affect the stability of the probe 3 and the electrode sensor 5, ensuring the accuracy of detecting the sample culture status inside the culture bottle 4.

[0031] In one specific embodiment, the lower cover 11 is fixed to the mouth of the culture flask 4 by a threaded connection.

[0032] In one specific embodiment, the junction box 18 is fixed to one corner of the first fixing frame 2 by a threaded connection.

[0033] In this embodiment, the probe 3 is fixed to the first fixing frame through one end of the sealing flexible connector 6, which reduces signal interference caused by the shaking of the probe 3 and the line, improves the detection accuracy of the sample culture status in the culture bottle 4, and thus improves the sample culture effect; moreover, reducing the shaking of the connecting line 7 improves the service life of the connecting line 7.

[0034] For further details, please refer to... Figure 4 The automatic feeding oscillating incubator also includes a second fixing frame 12, a feeding bottle 13, a feeding pipe 16, a feeding pipe 17, a feeding pump 14, and a feeding control box 15; the feeding bottle 13 and the feeding control box 15 are placed in the second fixing frame 12; the feeding pump 14 is provided on one side of the feeding control box 15; the feeding pump 14 is connected to the feeding bottle 13 through the feeding pipe 16; the feeding pump 14 is connected to the culture bottle 4 through the feeding pipe 17, and is used to replenish the culture bottle 4 with liquid.

[0035] Furthermore, the second fixing frame 12 is provided with an opening, through which the feed pump 14 replenishes the culture flask 4 with the required culture liquid.

[0036] In addition, the material replenishment control box 15 is connected to the hub box 18 via the connecting line 7.

[0037] In one specific embodiment, when the probe 3 detects that the culture flask 4 lacks the required culture solution, the probe 3 transmits a control signal to the feeding box 15 through the hub box 18. After receiving the signal, the feeding box 15 controls the feeding pump 14 to draw the required culture solution from the feeding bottle 13 and feeds the culture flask 4.

[0038] By setting up detection probe 3, the pH value, dissolved oxygen and other culture medium parameters of the culture are monitored in real time, and the feeding control system automatically feeds the culture bottle 4, ensuring the continuity and stability of the culture.

[0039] For preferred options, please refer to [the following]. Figure 5 The oscillation mechanism 8 includes an eccentric rotating shaft 19, a rocking plate 20, and a motor 21; the culture bottle 4 is placed on the rocking plate; the eccentric rotating shaft is connected to the rocking plate; the motor controls the eccentric rotating shaft to make the rocking plate rotate with different amplitudes.

[0040] In summary, in this embodiment, one end of the probe 3 connected to the connecting line 7 and one end of the sealing flexible connector 6 are fixed to the first fixing frame 2. The first fixing frame 2 is fixed inside the housing 1. When the oscillation mechanism 8 drives the culture bottle 4 to oscillate and shake, the other end of the sealing flexible connector 6 is shaken by the flexibility of the culture bottle 4, but the probe 3 will not shake. This reduces signal interference caused by the shaking of the probe 3 and the connecting line 7, improves the detection accuracy of the sample culture status in the culture bottle 4, and thus ensures the culture effect. Moreover, reducing the shaking of the connecting line 7 helps to improve its service life.

[0041] Furthermore, the electrode sensor 5 can monitor the pH value, dissolved oxygen and other culture medium parameters of the culture in real time. Through the monitoring and feedback of the above parameters, the feeding control box 15 controls the feeding pump 14 to feed the culture bottle 4, so as to ensure the continuity and stability of the culture.

[0042] Obviously, those skilled in the art can make various modifications and variations to this invention without departing from its spirit and scope. Therefore, if these modifications and variations fall within the scope of the claims of this invention and their equivalents, this invention also intends to include these modifications and variations.

Claims

1. An automatic feeding and shaking incubator, characterized in that, include: The enclosure, first mounting frame, probe, culture flask, electrode sensor, sealed flexible connector, junction box, connecting wires, and oscillation mechanism; The oscillation mechanism is installed inside the box, and the culture flask is fixed on the oscillation mechanism; The first fixing bracket and the junction box are fixed to the inner wall of the box. The sealing flexible joint is sealed and installed at the mouth of the culture bottle; The sealing flexible joint consists of an upper cover, a flexible joint, and a lower cover; The probe is fixed to the upper cover, which is fixed to the first fixing frame; the lower cover is sealed and installed at the mouth of the culture bottle. The flexible joint is connected between the upper cover and the lower cover; The probe is mounted on the sealing flexible joint; the probe is fixed to the first fixing frame through one end of the sealing flexible joint. An electrode sensor is installed inside the probe; one end of the electrode sensor passes through the sealed flexible connector and extends into the solution inside the culture flask, and the other end is connected to the probe; the electrode sensor is connected to the junction box through the probe and the connecting wire.

2. The automatic feeding and shaking incubator as described in claim 1, characterized in that, The flexible joint is made of silicone.

3. The automatic feeding and shaking incubator as described in claim 1, characterized in that, The lower cover is fixed to the mouth of the culture bottle by a threaded connection.

4. The automatic feeding shaking incubator as described in claim 1, characterized in that, The automatic feeding oscillating incubator also includes a second fixed frame, feeding bottle, feeding pipe, supply pipe, feeding pump, and feeding control box; The feeding bottle and the feeding control box are placed inside the second fixing frame; the feeding pump is provided on one side of the feeding control box; the feeding pump is connected to the feeding bottle through the feeding pipe; the feeding pump is connected to the culture bottle through the feeding pipe for replenishing the culture bottle with liquid.

5. The automatic feeding oscillating incubator as described in claim 4, characterized in that, The second mounting bracket has an opening through which the feed pump replenishes the culture flask with the required culture solution.

6. The automatic feeding and shaking incubator as described in claim 4, characterized in that, The material replenishment control box is connected to the junction box via the connecting cable.

7. The automatic feeding shaking incubator as described in claim 1, characterized in that, The probe is fixed to the first mounting bracket by a threaded connection.

8. The automatic feeding shaking incubator as described in claim 1, characterized in that, The oscillation mechanism includes an eccentric rotating shaft, a rocker plate, and a motor; The culture flask is placed on the rocking plate; the eccentric rotating shaft is connected to the rocking plate; the motor controls the eccentric rotating shaft to make the rocking plate rotate with different amplitudes.

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