A pathological section staining device

Abstract

The application discloses a pathological section dyeing equipment and belongs to the technical field of biological medical equipment. The pathological section dyeing equipment comprises a slide placing and heating device, a dyeing treatment device and a moving device. The slide placing and heating device comprises a device main body and a temperature adjusting assembly. The device main body comprises a slide table, and the slide table provides a slide placing position. The temperature adjusting assembly is used for heating and / or cooling the slide. The dyeing treatment device comprises a mounting main body and a first liquid adding module mounted on the mounting main body. The moving device is used for adjusting the relative position between the dyeing treatment device and the slide table. The pathological section dyeing equipment has the advantages of simple structure, good reagent adding effect and good temperature control effect.

Description

TECHNICAL FIELD

[0001] The present application relates to the field of biomedical equipment, in particular to a pathological section dyeing equipment. BACKGROUND

[0002] In the related art, the pathological section dyeing method is usually completed by manual operation, which has high requirements on the operation technology of the operator and is prone to inconsistent dyeing effects. Although the current pathological section dyeing equipment can automatically add reagents and perform other operations, different operation steps need to move the glass slide to different stations in the pathological section dyeing process, and the equipment structure is complex. SUMMARY

[0003] The purpose of the embodiments of the present application is to provide a pathological section dyeing equipment with simple structure, good reagent adding effect and good temperature control effect.

[0004] To achieve the above purpose, the following technical solutions are adopted in the present application:

[0005] A pathological section dyeing equipment comprises:

[0006] A glass slide placing and heating device comprises a device main body and a temperature adjusting assembly, the device main body comprises a slide table, and the slide table provides a glass slide placing position; the temperature adjusting assembly is used for heating and / or cooling the glass slide; a dyeing processing device comprises a mounting main body and a first liquid adding module mounted on the mounting main body; the first liquid adding module is used for adding reagents to the glass slide; a moving device is used for moving the position of the dyeing processing device and / or the glass slide placing and heating device, so that the dyeing processing device moves relative to the glass slide placing and heating device.

[0007] Preferably, an inner cavity is formed in the interior of the device main body; the slide table is provided with a first communication port in communication with the inner cavity, and the slide table comprises a support portion which provides a glass slide placing position; the temperature adjusting assembly comprises a heater and a blowing device; the heater is arranged in the inner cavity, and a first flow space is formed between the heater and the support portion; the blowing device is used for blowing air flow into the inner cavity.

[0008] Preferably, the blowing device is a cross-flow fan.

[0009] Preferably, the glass slide placing and heating device comprises a flow guide plate, and the flow guide plate is connected with the device main body; a second flow space is formed between the flow guide plate and the device main body, or the flow guide plate is provided with the second flow space; the device main body is provided with a flow port, and the second flow space communicates with the inner cavity through the flow port; the flow guide plate is used for guiding the gas in the inner cavity to the side of the slide table away from the inner cavity.

[0010] As preferred, the slide stage comprises a flow guide groove arranged at the side of the support part, the slide stage is provided with one or more flow guide grooves; the end of the slide stage forms a slot opening of the flow guide groove, the slot opening is used for the fluid in the flow guide groove to flow out.

[0011] As preferred, the first liquid adding module is an atomization device, the atomization device comprises an atomization sheet, the atomization sheet is a microporous atomization sheet, the atomization device comprises a first mist outlet, the atomization device sprays out the liquid atomized by the atomization sheet through the first mist outlet.

[0012] As preferred, the first liquid adding module is an atomization module, the atomization module is detachably connected with the device main body; the atomization module comprises a shell, a base and an atomization sheet; the atomization sheet is arranged between the shell and the base; the inside of the shell forms a liquid cavity, the shell is provided with a first opening in communication with the liquid cavity; the base is connected with the shell; the base is provided with a first mist outlet; the atomization sheet comprises an atomization area, the atomization area is provided with a plurality of atomization holes; one side of the atomization area is aligned with the first opening, and the other side is aligned with the first mist outlet.

[0013] As preferred, the dyeing treatment device comprises a second liquid adding module, the second liquid adding module is mounted on the mounting main body; the first liquid adding module is an atomization module, the atomization module comprises a first mist outlet, the atomization module is used for atomizing and spraying out the liquid; the second liquid adding module comprises a liquid adding pipe and a liquid pump, the liquid pump is connected with the liquid adding pipe, the liquid adding pipe comprises a liquid outlet; the first mist outlet and the liquid outlet are arranged at the same side of the dyeing treatment device.

[0014] As preferred, the dyeing treatment device comprises a blowing module; the blowing module is mounted on the mounting main body; the blowing module is provided with an air outlet, the blowing module is used for blowing out the airflow outwardly through the air outlet; the first mist outlet, the liquid outlet and the air outlet are formed at the same side of the dyeing treatment device.

[0015] As preferred, the moving device comprises a rack and a driving device; the rack comprises a first rack body and a second rack body, the device main body of the slide placing and heating device is mounted on the first rack body; the dyeing treatment device is movably connected with the second rack body, the second rack body comprises an x-axis guide rail and a y-axis guide rail, the driving device drives the dyeing treatment device to move along the x-axis guide rail or the y-axis guide rail.

[0016] The pathological section dyeing equipment has the advantages that the slide placing and heating device has the functions of bearing the slide and temperature control, the relative position between the dyeing treatment device and the slide table can be adjusted through the driving of the moving device, multiple steps can be implemented on a single station, the overall structure is simple, and good reagent adding effect and good temperature control effect are achieved. BRIEF DESCRIPTION OF DRAWINGS

[0017] The application will be further described in detail below according to the drawings and embodiments.

[0018] Figure 1 Fig. 1 is a structural schematic view of a pathological section dyeing equipment according to an embodiment of the application (a slide placing and heating device is not shown in the figure);

[0019] Figure 2 Fig. 2 is a structural schematic view of a pathological section dyeing equipment according to another embodiment of the application;

[0020] Figure 3 Fig. 3 is an implementation of part of a pathological section dyeing process;

[0021] Figure 4 Fig. 4 is a structural schematic view of a slide placing and heating device according to a first embodiment of the application;

[0022] Figure 5 Fig. 5 is a sectional view of the slide placing and heating device according to the first embodiment of the application (a blowing device is not shown in the figure);

[0023] Figure 6 Fig. 6 is a structural schematic view of a device main body in a second embodiment of the slide placing and heating device according to the application;

[0024] Figure 7 Fig. 7 is a sectional view of the device main body in the second embodiment of the slide placing and heating device according to the application;

[0025] Figure 8 Fig. 8 is a structural schematic view of a slide table in the second embodiment of the slide placing and heating device according to the application; Figure 7

[0026] Figure 9 Fig. 9 is an exploded structural schematic view of the slide table in the second embodiment of the slide placing and heating device according to the application;

[0027] Figure 10 Fig. 10 is a sectional view of the slide table in the second embodiment of the slide placing and heating device according to the application;

[0028] Figure 11 Fig. 11 is a structural schematic view of a microporous sheet of an atomizing sheet in a first liquid adding module according to an embodiment of the application; ​

[0029] Figure 12 Partial view of one of the micro-porous sheets of the atomizing sheet in the first liquid adding module according to the embodiments of the present application;

[0030] Figure 13 Cross-sectional view of the first liquid adding module according to the embodiments of the present application;

[0031] Figure 14 Enlarged view of B in Figure 13

[0032] Figure 15 Structure view of the dyeing treatment device according to the embodiments of the present application;

[0033] Figure 16 Enlarged view of C in Figure 15

[0034] In the figure: 10, glass slide placing heating device; 11, device main body; 1101, inner cavity; 11021, first communication port; 11022, second communication port; 11023, third communication port; 1103, first flow-through space; 1104, flow-through port; 1105, second flow-through space; 111, slide stage; 1111, top plate; 1112, slide support; 1113, flow guide groove; 1114, groove opening; 1115, frame body; 1116, liquid guide surface; 1117, rib; 1118, limiting structure; 112, side plate; 113, bottom plate; 12, heater; 13, air blowing device; 14, flow guide plate; 141, first plate part; 142, second plate part; 20, dyeing treatment device; 21, mounting main body; 22, first liquid adding module; 221, housing; 2211, liquid cavity; 2212, first opening; 2213, guide surface; 222, atomizing sheet; 2221, piezoelectric ceramic sheet; 2222, micro-porous sheet; 2223, mounting part; 2224, liquid receiving part; 2225, atomizing area; 2226, edge area; 2227, atomizing hole; 223, base; 2231, first atomizing port; 23, liquid adding pipe; 231, liquid outlet; 24, air blowing module; 241, air outlet; 30, moving device; 311, first frame body; 312, second frame body; 3121, y-axis guide rail; 3122, x-axis guide rail; 313, driving device; 80, power supply box; 90, glass slide. DETAILED DESCRIPTION

[0035] ​​In order to make the technical problems solved by the present application, the technical solutions adopted and the technical effects achieved more clear, the technical solutions of the embodiments of the present application will be further described in detail below with reference to the drawings. Obviously, the described embodiments are only part of the embodiments of the present application, rather than all the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without creative work fall within the protection scope of the present application.

[0036] In the description of the present application, unless explicitly defined and limited otherwise, the terms "connected", "fixed" should be understood broadly, for example, can be fixed connection, can also be detachable connection or integral; can be mechanical connection, can also be electrical connection; can be directly connected, can also be indirectly connected through an intermediate medium, can be the internal communication of two elements or the interaction relationship of two elements. For those skilled in the art, the specific meaning of the above terms in the present application can be understood according to the specific circumstances.

[0037] In the present application, unless explicitly defined and limited otherwise, "on" or "under" of the first feature to the second feature can include that the first and second features are in direct contact, or that the first and second features are not in direct contact but are in contact through another feature between them. Moreover, "on", "above" and "above" of the first feature to the second feature includes that the first feature is directly above and obliquely above the second feature, or only indicates that the horizontal height of the first feature is higher than that of the second feature. "Below", "below" and "below" of the first feature to the second feature includes that the first feature is directly below and obliquely below the second feature, or only indicates that the horizontal height of the first feature is less than that of the second feature.

[0038] In pathological detection, the tissue section (paraffin section) sample to be detected is placed on a glass slide, and then detection and identification are realized by adding reagents and other operations. The commonly used pathological detection is mainly immunohistochemical pathological detection. Immunohistochemistry, IHC is a new technology developed on the basis of histochemical methods combined with immunological theories and technologies, combining the theories and methods of immunology, histology and biochemistry. Using the core of immunology, i.e. the principle of specific binding of antigen and antibody, tracking antigen with antibody labeled with enzyme, metal ion, isotope, etc., displaying a certain color through chemical reaction of specific antibody labeled on the combined antigen, and observing the staining results by microscope, fluorescence microscope, electron microscope, so as to achieve the purpose of detecting antigen. The main steps in the process of immunohistochemical staining include: baking, dewaxing, hydration, antigen repair, or optional addition of peroxidase blocking, staining, re-staining, etc. In the pathological detection process, for example, in the antigen repair step, the temperature is required, at which time the glass slide, the sample on the glass slide and the reagent on the glass slide need to be heated.

[0039] In the related art, in the pathological detection process, manual operation or semi-automatic or automatic operation through a pathological staining device is generally adopted. The manual operation has the disadvantage of low efficiency. The pathological staining device in the related art has the following problems:

[0040] Firstly, a plurality of stations are provided, and the overall size of the device is large. When the steps of baking, dewaxing, hydration, antigen repair, staining, and restaining are performed, different steps are performed on different stations, different devices are arranged on the different stations, the device structure is complex, a plurality of stations occupy a large space, and the overall size of the device is large.

[0041] Secondly, in terms of temperature control, there are problems of waste of reagents and inaccurate temperature control. In the steps of baking and antigen repair, certain heating needs to be performed, and the commonly used heating methods are water bath heating or direct contact heating. In the water bath heating, the slide is immersed in the required reagent, and the temperature of the reagent needs to meet the experimental temperature, but boiling is easy to cause the sample to separate or be damaged. This method needs to use a large amount of reagents, and the reagents are wasted. In the direct contact heating, the heating piece at the bottom of the slide is contacted to complete the heating, and the required reagents are less than those in the water bath heating. However, since the contact between the heating piece and the slide is not uniform, and the heating area of the heating piece itself is not balanced, the temperature is difficult to control. Uneven heating can cause local bubbles in the sample, thereby affecting the detection and experimental effect, and the slide is easy to dry.

[0042] Thirdly, in terms of reagent addition, it is difficult to meet the requirements of adding a plurality of reagents. In the staining step, the addition of the first antibody and the second antibody is generally performed by using a dropper or a pipette to add reagents. However, there are problems such as a large minimum amount of reagents added at one time, concentrated addition of a small amount of staining reagents, and easy overflow of the slide and residue on the slide which may dilute the concentration of a kind of staining reagent, and the need to use more cleaning reagents for cleaning.

[0043] Fourthly, in the cleaning step, there are problems of waste of reagents and incomplete cleaning. In the related art, water bath cleaning or siphon cleaning is generally adopted. The water bath cleaning needs to replace the solution, and a large amount of solution reagent is wasted. The siphon cleaning cannot be cleaned completely, and residues are easy to remain, thereby affecting the next step.

[0044] The present application solves the above problems of the pathological staining device in the related art by the following means:

[0045] The problems of "multiple stations, complex overall structure of equipment, and large volume" are solved by the following embodiment one and embodiment six. The slide placing and heating device 10 of the present application has the functions of bearing the slide 90 and heating the slide 90, and cooperates with the movable staining treatment device 20, so that the slide 90 does not need to be moved from one station to another station by using a moving mechanism, and multiple or all steps in "baking, dewaxing and hydrating, antigen repairing, staining, and restaining" can be completed in a single station, thereby saving the overall volume of the equipment.

[0046] The problems of "waste of reagent and inaccurate temperature control" in temperature control are solved by the following embodiment two. The slide placing and heating device 10 of the present application provides a wind control environment, and uses a small range of wind circulation to cooperate with the heater 12 or the refrigerator for temperature adjustment. Compared with the direct contact heating method, the heating is more uniform and the temperature control is more accurate. Compared with the water bath heating method, the reagent is saved.

[0047] The problems of "inability to meet the requirements of multiple reagent addition" in reagent addition are solved by the following embodiment three. The staining treatment device 20 of the present application can add reagents by atomizing spraying through the first liquid adding module 22. Compared with the immersion staining method, the reagent usage can be greatly reduced, and compared with the drop liquid staining method, the reagent can be more accurately covered and infiltrated into the sample.

[0048] The problems of "inability to meet the requirements of multiple reagent addition" in reagent addition are solved by the following embodiment four. The staining treatment device 20 of the present application can be provided with a second liquid adding module in addition to the first liquid adding module 22, and different liquid adding modules can be used to add reagents in different steps.

[0049] The problems of "waste of reagent or incomplete cleaning" in the cleaning step are solved by the following embodiment five. The staining treatment device 20 of the present application can realize the cleaning of flowing cleaning liquid by cooperating the blowing module 24, the second liquid adding module, and the moving device 30, and the cleaning liquid can be scraped by blowing, so that the sample can be cleaned completely by a small amount of reagent, and the cleaning step is reduced to have no residue or to have most of the residue.

[0050] The following embodiment six is an embodiment of the moving device 30.

[0051] The following embodiment one to embodiment six are described in detail.

[0052] Embodiment one:

[0053] Please refer to Figures 1 to 16 The pathological section staining equipment of the present application includes the slide placing and heating device 10, the staining treatment device 20, and the moving device 30. Figure 1In the embodiment, a structural diagram of the pathological section staining device without the slide placing and heating device 10 is shown. Figure 2 In the embodiment, a placing position of the slide 90 when the device is applied is shown.

[0054] The slide placing and heating device 10 is used to carry the slide 90 and adjust the temperature of the slide 90, a sample on the slide 90, and a reagent on the slide 90. The staining treatment device 20 is used to add various reagents to the slide 90 or the sample. The moving device 30 is connected with the staining treatment device 20 to adjust the position of the staining treatment device 20, so as to move the staining treatment device 20 to or away from above the slide stage 111.

[0055] In the embodiment, the slide placing and heating device 10 includes a device main body 11 and a temperature adjusting assembly. The device main body 11 includes a slide stage 111, which provides a placing position of the slide 90. The temperature adjusting assembly is used to heat and / or cool the slide 90. The staining treatment device 20 includes a mounting main body 21 and a first liquid adding module 22, which is mounted to the mounting main body 21. The first liquid adding module 22 includes a liquid adding opening and is used to add a reagent to the slide 90 through the liquid adding opening.

[0056] In the embodiment, the slide placing and heating device 10 has the functions of carrying the slide 90 and adjusting the temperature of the slide 90 and materials thereon. When pathological staining is performed, the slide 90 is placed on the slide stage 111 of the slide placing and heating device 10, and the moving device 30 moves the staining treatment device 20 to above the slide stage 111. Through the cooperation of the temperature adjusting assembly and the staining treatment device 20, the slide 90 does not need to be moved to different stations during pathological staining, and the slide 90 can meet the requirements of reagent addition and temperature control in the operation process in the same station, so that the whole pathological staining process is more simple and convenient. The pathological staining system has high practicability, high reliability, and a wider application range.

[0057] For example, in the related art, when pathological section staining is performed, the slide 90 needs to be heated in a special heating station, and after heating is completed, the slide 90 needs to be moved to the next station for reagent addition, which causes the whole detection experiment to be troublesome. However, in the pathological section staining device, the slide 90 can be placed on the slide placing and heating device 10 in the steps of baking, deparaffinization and hydration, antigen repair, staining, and restaining in the pathological staining process, and the slide 90 does not need to be moved to another station, so that the pathological staining process is more simple.

[0058] The drying step, the antigen repairing step, the staining step and the re-staining step of the pathological staining process can be implemented on the slide placing heating device 10. As an example, Figure 3 The method related to several operation steps in the pathological staining method based on the immunohistochemical technology is provided. It should be noted that, Figure 3 The pathological staining method in the embodiment is not a limitation of the present application.

[0059] The action of placing the slide 90 can be manually performed or automatically placed by an electric device.

[0060] In the embodiment, the moving device 30 is configured to drive the staining processing device 20 to move, rather than the slide placing heating device 10, which is beneficial to the technical solutions and effects in the embodiment six by using the same staining processing device 20.

[0061] In other embodiments, the moving device 30 can also be used to move the slide placing heating device 10 to move the slide stage 111 to the lower side of the staining processing device 20; as long as the relative movement between the staining processing device 20 and the slide placing device is achieved, the position adjustment between the liquid adding mold and the slide stage 111 can be achieved.

[0062] The moving device 30 can be, but is not limited to, a gantry assembly, a mechanical hand, and a hanging rail.

[0063] In the embodiment, the pathological section staining device further includes a power box 80, and the electrical devices in the slide placing heating device 10 and the electrical devices in the staining processing device 20 are respectively electrically connected to the power box 80.

[0064] Embodiment two:

[0065] The technical solutions in the embodiment one are included. Please refer to Figures 4 to 10 The embodiment provides an implementation of the slide placing heating device 10.

[0066] In the related art, the water bath method or the direct heating method is generally used to heat the slide 90 to achieve temperature control, and there is a waste of reagents or uneven heating.

[0067] In the embodiment, the slide placing heating device 10 provides a wind control environment, and through the cooperation of the blowing device 13 and the temperature adjusting piece, the circulation heating is achieved, the reagents are not wasted, the heating is more uniform, the slide is not easily dried, and the slide 90 does not need to be soaked in the reagent or directly contacted with the heater 12. Compared with the water bath method, the use of the slide placing heating device 10 to heat the slide 90 can save 70% to 90% of the reagents.

[0068] The temperature adjusting assembly comprises a heater 12 and a blowing device 13, the slide 90 is placed on the slide stage 111, at least a part of the slide 90 is in the same space with the heater 12, the heater 12 is a heating device, the blowing device 13 blows air to the aforementioned space to realize thermal cycling, the heater 12 does not directly contact the slide 90, the slide 90 is uniformly heated and is not easy to bubble.

[0069] Please refer to Figure 4 、 Figure 5 、 Figure 7 、 Figure 8 , the air circulation is realized by the following way:

[0070] The heater 12 is connected with the device main body 11, and the blowing device 13 is connected with the device main body 11.

[0071] The device main body 11 has an inner cavity 1101 inside, and the inner cavity 1101 is used as a heating chamber. The device main body 11 comprises a slide stage 111, and the slide stage 111 comprises a support part for providing a slide 90 placement position. When the slide 90 is placed, the support part of the slide stage 111 contacts and supports the slide 90. The slide stage 111 is provided with a first communication port 11021 which is in communication with the inner cavity 1101, and the first communication port 11021 is located inside the support part in the horizontal direction. When the slide 90 is placed on the slide stage 111, the outer edge position of the slide 90 is supported by the support part of the slide stage 111, and the lower part of the main body part of the slide 90 is the first communication port 11021.

[0072] The heater 12 is arranged in the inner cavity 1101, in other words, the heater 12 is arranged inside the device main body 11. As shown in the drawings, the first flow space 1103 is arranged between the heater 12 and the support part, so as to keep a certain distance between the slide 90 and the heater 12, and avoid direct contact between the heater 12 and the slide 90. The heater 12 can be but is not limited to an electromagnetic heater 12, a resistance heater 12, an infrared heater 12.

[0073] The blowing device 13 is used for blowing air flow to the inner cavity 1101, and the blowing device 13 is used for disturbing the gas in the inner cavity 1101.

[0074] The slide placing and heating device 10 is used, the slide 90 is placed on the slide table 111, the heater 12 and the blowing device 13 are started, the heater 12 radiates heat outward, the air in the inner cavity 1101 is heated, since the lower surface of the slide 90 is exposed by the first communication port 11021, the hot air acts on the slide 90, and the heating of the slide 90 is realized. The blowing device 13 disturbs the air in the inner cavity 1101, so that the hot air in the inner cavity 1101 is uniformly distributed, so that the temperature of each part of the inner cavity 1101 is more uniform, the heating of the slide 90 is more uniform, the dry piece situation can be avoided, the sample bubble situation caused by local temperature unevenness can be avoided, the heating effect is better, and the influence on other steps is smaller.

[0075] The slide placing and heating device 10 of the application, the heater 12 does not directly contact the slide 90, the inner cavity 1101 is realized by the cooperation of the heater 12 and the blowing device 13, the hot air circulation is realized, the accurate temperature control is realized, the reagent is not wasted, and the sample is prevented from being damaged due to the high temperature of the reagent. The slide placing and heating device 10 of the application, the heater 12 does not directly contact the slide 90, since the first flow space 1103 is reserved between the heater 12 and the slide 90, and the hot air in the inner cavity 1101 can be circulated by the blowing device 13, even if the bottom wall of the slide 90 is not flat, or the top of the heater 12 is not flat, or the heating of each region of the heater 12 is uneven, the slide 90 can be uniformly heated, and the dry piece situation can be avoided. The slide placing and heating device 10 of the application can realize temperature control by controlling the power of the heater 12, and the temperature control is simple.

[0076] In an embodiment, the slide placing and heating device 10 further comprises a refrigerator and a humidifier, by attaching a refrigeration sheet or setting other refrigerators on the blowing path of the blowing device 13, or in the inner cavity 1101, or in other positions, small range refrigeration circulation can also be realized by air flow. By setting a sprayer, a water tank and the like on the blowing path of the blowing device 13, the humidifier with the humidity increasing effect can be realized, and the humidification effect can be realized by air flow. The slide placing and heating platform of the application provides a wind control environment by the cooperation of the device main body 11 and the blowing device 13, and can realize more uniform and high-precision temperature control and humidity control through small range air circulation.

[0077] Taking the repair step in the pathological tissue staining process as an example, the repair liquid needs to be added to the sample on the slide 90, and the temperature of the repair environment needs to be around a degrees Celsius. If the water bath method is used for heating, the slide 90 needs to be soaked in the repair liquid at a degrees Celsius, and a large amount of repair liquid needs to be used, which leads to waste of solution reagents. The heating device of the present application is used for heating, only a small amount of repair liquid that can soak the sample on the slide 90 is needed, and heating can be realized through the cooperation of the heater 12 and the blowing device 13, which meets the temperature requirement of the repair step and saves solution reagents. Although the direct contact method is used for heating, it is relatively more economical than the water bath heating method, but it is easy to cause uneven heating, dry slides and other situations. The heating device of the present application is used for heating, which is more uniform, less likely to dry the slide and less likely to form bubbles.

[0078] The blowing device 13 is arranged outside the inner cavity 1101, and the blowing device 13 includes a bottom plate 113. The bottom plate 113 is provided with a second communication port 11022 that communicates with the inner cavity 1101. The heater 12 and the blowing device 13 are arranged on opposite sides of the bottom plate 113. In other words, the blowing device 13 is arranged on the side of the second communication port 11022 away from the heater 12. In this way, the blowing device 13 can conveniently send the airflow outside the inner cavity 1101 into the inner cavity 1101, which can complete the airflow disturbance in the inner cavity 1101 and avoid the blowing device 13 being too close to the heater 12, thereby avoiding damage caused by the blowing device 13 being too high in temperature. The blowing device 13 has lower requirements for heat resistance and lower cost, and can directly use the existing blowing device 13.

[0079] In the embodiment, the device main body 11 includes a side plate 112. The slide stage 111 and the bottom plate 113 are arranged on opposite sides of the side plate 112. The inner cavity 1101 for accommodating the heater 12 is formed between the side plate 112 and the bottom plate 113.

[0080] In other embodiments, the device main body 11 can also have other shapes.

[0081] In order to make the temperature of the air in the first flow space 1103 reach the target temperature faster and make the circulation effect of the hot air in the inner cavity 1101 better, the first communication port 11021 and the second communication port 11022 are arranged on opposite sides of the device main body 11. In the embodiment, the slide stage 111 is located on the upper side of the heater 12, and the heater 12 is located on the upper side of the blowing device 13.

[0082] In other embodiments, on the basis that the blowing device 13 is arranged outside the inner cavity 1101, the second communication port 11022 can also be arranged on the side plate 112 of the device main body 11.

[0083] In other embodiments, the blowing device 13 can also be arranged inside the inner cavity 1101.

[0084] In other embodiments, the blowing device 13 can also be arranged inside the inner cavity 1101, and a second communication port 11022 communicating with the inner cavity 1101 is arranged on the device main body 11, as long as the gas in the inner cavity 1101 can be disturbed when the blowing device 13 is started, so that the heat in the inner cavity 1101 is more evenly distributed.

[0085] In order to enhance the efficiency of air circulation in the inner cavity 1101 to achieve a more uniform heating effect, the blowing device 13 adopts a cross-flow fan, also known as a cross-flow fan or a cross-flow fan. Compared with using other ordinary axial flow fans as blowing devices 13, using cross-flow fans as blowing devices 13 can achieve better heat circulation effect.

[0086] The cross-flow fan can realize the functions of directional air intake and directional air outlet. The airflow sent by the cross-flow fan is a vortex, which has a better disturbance effect on the gas in the inner cavity 1101, improves the circulation effect of the gas in the inner cavity 1101, improves the heat circulation efficiency, and makes the heating of the glass slide 90 more uniform. Moreover, the cross-flow fan has simple structure, small volume, high dynamic pressure coefficient and long distance.

[0087] In other embodiments, a common axial flow fan (such as a common fan) can also be used as the blowing device 13.

[0088] The device main body 11 includes a bottom plate 113, and the slide stage 111 and the bottom plate 113 are arranged on opposite sides of the device main body 11; the second communication port 11022 is arranged on the bottom plate 113. In order to make the air supply efficiency of the blowing device 13 higher, the second communication port 11022 is configured to gradually expand from the side close to the blowing device 13 to the side close to the heater 12, in other words, the air outlet end of the second communication port 11022 is larger than the air inlet end. In this way, in the case that the width of the second communication port 11022 is smaller than the width of the heater 12, the second communication port 11022 is gradually expanded, so that the gas is blown outward under the guidance of the inner wall of the communication port during the process of passing through the second communication port 11022, so that the airflow can be blown to the outside of the heater 12, and a better heat circulation effect is achieved.

[0089] In order to facilitate the installation of the blowing device 13, a mounting plate connected with the device main body 11 is arranged, and the blowing device 13 is mounted and fixed on the mounting plate.

[0090] The slide stage 111 can be configured at least in the following way:

[0091] Configuration mode one of the slide stage 111: as Figures 6 to 10As shown, the slide stage 111 is a split structure. The slide stage 111 includes a top plate 1111 and a slide support 1112, a first communication port 11021 is formed on the top plate 1111, and a support portion is formed on the slide support 1112; the slide support 1112 is arranged on the side of the top plate 1111 away from the heater 12, and the top plate 1111 supports the slide support 1112. The slide support 1112 and the top plate 1111 are detachably connected.

[0092] In this way, the slide stage 111 is configured, which facilitates machining different parts with different shapes on the top plate 1111 or the slide support 1112 according to requirements, to provide different support, gas flow guiding, and liquid flow guiding effects. It is convenient to replace the top plate 1111 or the slide support 1112 with different structures according to different requirements, or to clean the slide stage 111.

[0093] The second configuration mode of the slide stage 111 is as shown in Figures 4 to 5 The slide stage 111 is a one-piece structure. The device main body 11 includes a side plate 112, and the slide stage 111 is arranged on the top of the side plate 112, and the slide stage 111 is connected with the side plate 112.

[0094] In order to uniformly heat the slide 90, the slide placing and heating device 10 includes a flow guide plate 14. The flow guide plate 14 is connected with the device main body 11, and the flow guide plate 14 is connected with the side plate 112 of the device main body 11.

[0095] The device main body 11 is provided with an overflow port 1104, and the inner cavity 1101 communicates with the second overflow space 1105 through the overflow port 1104. A part of the flow guide plate 14 is located on the side of the slide stage 111 away from the inner cavity 1101, in other words, a part of the flow guide plate 14 is located on the top of the slide stage 111. The flow guide plate 14 is used to guide the gas in the inner cavity 1101 to the side of the slide stage 111 away from the inner cavity 1101, in other words, the flow guide plate 14 is used to guide the gas flowing out of the overflow port 1104 to the upper side of the slide stage 111.

[0096] After the gas in the inner cavity 1101 is heated, it can flow to the second overflow space 1105 through the overflow port 1104, and then flow to the upper side of the slide 90 of the slide stage 111 from the second overflow space 1105. In this way, when the slide placing and heating device 10 heats the slide 90, the bottom surface of the slide 90 exchanges heat with the hot gas in the inner cavity 1101 to achieve heating, and the top surface of the slide 90, the sample carried by the slide 90, and the reagent carried by the slide 90 exchange heat with the hot gas above the slide 90 to achieve heating, which can achieve more uniform heating of the sample and the reagent, and the heating effect is better.

[0097] Please continue to refer to Figures 4 to 6The guide plate 14 includes a first plate portion 141 and a second plate portion 142. The first plate portion 141 is connected to the side plate 112, and the second plate portion 142 is connected to the first plate portion 141. The first plate portion 141 is vertical, and the second plate portion 142 extends inward from bottom to top. The second plate portion 142 can be a curved plate or an inclined straight plate. The second flow space 1105 includes a first gap between the first plate portion 141 and the device body 11, and a second gap between the second plate portion 142 and the device body 11. The first plate portion 141 is used to guide the gas flowing out of the flow port 1104 upward, and the second plate portion 142 is used to guide the gas in the first space inward.

[0098] In this embodiment, the guide plate 14 and the side plate 112 are integrally formed. In other embodiments, the guide plate 14 and the side plate 112 may also be separate structures, and then connected by fasteners, welding, adhesive bonding, snap-fit ​​connection, etc.

[0099] In other embodiments, the flow guide plate 14 can also be connected to the plate stage 111 of the device body 11, as long as a second flow space 1105 can be formed between the flow guide plate 14 and the device body 11.

[0100] The flow port 1104 of the main body 11 of the device can be configured in at least one of the following ways:

[0101] Setting method 1 for flow port 1104: (e.g.) Figure 7 , Figure 8 As shown, the substrate stage 111 includes a top plate 1111 and a substrate support 1112. A first connecting port 11021 is formed on the top plate 1111, and a support portion is formed on the substrate support 1112. The substrate support 1112 is located on the side of the top plate 1111 away from the heater 12. The top plate 1111 supports the substrate support 1112. An overflow port 1104 is formed between the substrate support 1112 and the top plate 1111.

[0102] Method 2 for setting up port 1104: (e.g.) Figure 5 As shown, the main body 11 of the device includes a side plate 112, a slide stage 111 is disposed on the top of the side plate 112, the slide stage 111 is connected to the side plate 112, and an outlet 1104 is formed between the slide stage 111 and the side plate 112.

[0103] To guide more hot gas to the top of the stage 111 and improve the heating effect, guide plates 14 are provided on at least two sides of the main body 11. Guide plates 14 are provided on opposite sides in the width direction of the main body 11, and correspondingly, flow ports 1104 are also formed on opposite sides in the width direction of the main body 11.

[0104] A portion of the gas in the inner cavity 1101 is blown by the left over-flow port 1104 to the flow guide surface of the left flow guide plate 14, and the left flow guide plate 14 first guides the gas upward and then guides the gas to the right, so as to blow the hot gas to the upper side of the slide glass 90. Another portion of the gas in the inner cavity 1101 is blown by the right over-flow port 1104 to the flow guide surface of the right flow guide plate 14, and the right flow guide plate 14 first guides the gas upward and then guides the gas to the left, so as to blow the hot gas to the upper side of the slide glass 90. By arranging the over-flow port 1104 and the flow guide plate 14 on the opposite sides of the device body 11, the hot gas can be blown to the upper side of the slide glass 90 more evenly, and the uniform heating of the upper side can be realized. Compared with arranging only one flow guide plate 14, the heating effect is more uniform by arranging the flow guide plates 14 on the opposite sides.

[0105] In the embodiment, the flow guide plates 14 are arranged on the opposite sides in the width direction of the device body 11, and compared with arranging the flow guide plates 14 on the opposite sides in the length direction of the device body 11, the flow guide plates 14 with larger surface area can be provided, and the hot gas flow guide effect is better.

[0106] In other embodiments, the flow guide plates 14 can also be arranged on the opposite sides in the length direction of the device body 11.

[0107] On the basis of arranging the flow guide plates 14 on the opposite sides, in order to avoid the flow guide plates 14 from hindering the operation of adding reagents to the slide glass 90, the flow guide plates 14 on the opposite sides are arranged as follows:

[0108] One end of each of the flow guide plates 14 on the opposite sides is connected to the device body 11, and the other end is a free end; the free ends of the flow guide plates 14 on the opposite sides form an avoidance gap; a projection area of a projection of the avoidance gap to a plane in which the first communication port 11021 is located is an avoidance projection area, and at least a portion of the avoidance projection area is located in the first communication port 11021.

[0109] In the embodiment, the avoidance projection area completely falls into the first communication port 11021, in other words, the upper side of the main body part of the slide glass 90 for carrying the sample is the avoidance gap, so that the flow guide plates 14 do not block the upper side of the main body part of the slide glass 90, and when the reagent needs to be added to the sample in the repair step, the dyeing step and the re-dyeing step of the pathological dyeing process, the liquid can be applied downward through the liquid adding module above the avoidance gap, and the liquid falls on the slide glass 90 through the avoidance gap, so as to realize the reagent adding.

[0110] By arranging the avoidance gap, after the slide glass 90 is placed on the slide glass stage 111, the heating of the slide glass 90 can be realized, and when the reagent needs to be added to the slide glass 90, the slide glass stage 111 does not need to be pulled out, so that the whole detection and experiment process is simpler.

[0111] In other embodiments, the avoidance gap can also not be provided, and the slide table 111 can be pulled out of the device main body 11 when reagents need to be added.

[0112] Please continue to refer to Figures 7 to 10 In order to make the scope of application of the slide placing heating device 10 wider, and to facilitate various steps after the slide 90 is placed on the slide placing heating device, a guide groove 1113 is arranged on the slide placing heating device 10. The guide groove 1113 is formed on the outer side of the support portion, which refers to the side away from the center line of the first communication port 11021. The guide groove 1113 is used to receive the liquid overflowing from the slide 90.

[0113] The slide table 111 includes a guide groove 1113 arranged on the lateral side of the support portion. In this embodiment, the guide groove 1113 is arranged on each of the opposite sides in the width direction of the slide placing heating device 10. In this way, the guide groove 1113 can be provided on each of the left and right sides of the slide 90.

[0114] In order to facilitate the timely discharge of the liquid in the guide groove 1113, the end of the slide table 111 is formed with a groove opening 1114 of the guide groove 1113. The groove opening 1114 is used for the liquid in the guide groove 1113 to flow out. The liquid in the guide groove 1113 can flow to the groove opening 1114 automatically or passively, so as to be discharged out of the guide groove 1113.

[0115] Since the slide table 111 is provided with the guide groove 1113, when the amount of reagent added to the slide 90 is too large, the reagent overflowing from the slide 90 can enter the guide groove 1113 and be discharged. When it is necessary to clean the slide 90 with a cleaning agent, the cleaning agent can be discharged to the guide groove 1113 and discharged. Through the arrangement of the guide groove 1113, the scope of application of the slide placing heating device 10 is wider, and when the slide 90 is cleaned, it is also unnecessary to transfer the slide 90 to another station for placement.

[0116] In other embodiments, the guide groove 1113 can also be arranged on only one side, or can be arranged around a circle.

[0117] In other embodiments, the groove opening 1114 can also not be arranged on the end of the slide table 111, that is, the groove opening of the guide groove 1113 can also not be arranged. The liquid in the guide groove 1113 can be discharged by manually disassembling the slide table 111, absorbing the liquid in the guide groove 1113 with a water-absorbing cotton, or sucking the liquid in the guide groove 1113 with a suction pipe.

[0118] In order to facilitate the arrangement of the guide groove 1113, and to make the excess liquid on the slide 90 enter the guide groove 1113 in time and smoothly, the slide table 111 is arranged in the following manner:

[0119] The slide table 111 comprises a top plate 1111 and a slide support 1112; the first communication port 11021 is arranged on the top plate 1111, and the support part is arranged on the slide support 1112; the slide support 1112 is arranged on the side of the top plate 1111 away from the inner cavity 1101, and the top plate 1111 supports the slide support 1112; the flow guide groove 1113 is formed on the top plate 1111, and the groove opening 1114 of the flow guide groove 1113 is formed on the end of the top plate 1111. The flow guide groove 1113 is open at the top, and liquid can flow into and fall into the flow guide groove 1113 from top to bottom.

[0120] In this way, the slide table 111 is located above the top plate 1111, the flow guide groove 1113 is formed on the top plate 1111, and the flow guide groove 1113 is located below the slide table 111, so that the liquid on the slide glass 90 can be easily guided into the flow guide groove 1113. Moreover, by arranging the slide support 1112 separately from the top plate 1111, it is convenient to directly process the flow guide groove 1113 with an open top on the top plate 1111. In addition, the top plate 1111 is processed with the flow guide groove 1113, and the slide support 1112 can be detachably connected to the top plate 1111. The slide support 1112 is disassembled at regular intervals, which facilitates regular cleaning of the flow guide groove 1113 and prevents the flow guide groove 1113 from accumulating dirt.

[0121] In other embodiments, the slide table 111 can also be an integral structure, and the flow guide channel can be punched in the interior of the slide table 111 by punching.

[0122] Please continue to refer to Figure 10 In order to automatically discharge the liquid entering the flow guide groove 1113, the flow guide groove 1113 is arranged obliquely. In this embodiment, the flow guide groove 1113 comprises a first groove part and a second groove part opposite to each other, and the groove opening 1114 is formed on the second groove part of the flow guide groove 1113; the flow guide groove 1113 is inclined downward from the first groove part to the second groove part. In this way, the liquid entering the flow guide groove 1113 can flow along the flow guide groove 1113 to the groove opening 1114 under the action of gravity, so as to be discharged from the slide glass placing and heating device 10.

[0123] In other embodiments, the flow guide groove 1113 can also be arranged horizontally, and the liquid in the flow guide groove 1113 can be discharged from the groove opening 1114 by suction or blowing.

[0124] Please continue to refer to Figure 9 On the basis of arranging the flow guide groove 1113 on the top plate 1111, in order to more smoothly guide the liquid on the slide glass 90 into the flow guide groove 1113, the slide support 1112 is configured as follows:

[0125] The slide support 1112 comprises a frame 1115 connected with the support part. In the present embodiment, the frame 1115 surrounds the support part in the horizontal direction, in other words, the frame 1115 surrounds the slide 90. The top of the frame 1115 is provided with a liquid guide surface 1116. The liquid guide surface 1116 is used to receive the liquid flowing outwards on the slide 90, and is used to guide the liquid downwards and outwards, so that the liquid flows into the flow guide groove 1113.

[0126] The liquid guide surface 1116 is located above the flow guide groove 1113, and the liquid guide surface 1116 is inclined from the side close to the support part to the side away from the support part to the direction close to the flow guide groove 1113. In the present embodiment, the liquid guide surface 1116 is inclined downwards from the side close to the support part to the side away from the support part. The liquid guide surface 1116 is used to guide the liquid into the flow guide groove 1113.

[0127] After the slide 90 is placed on the support part of the slide platform, the upper surface of the slide 90 is higher than the liquid guide surface 1116. When there is more liquid, the liquid flows to the liquid guide surface 1116 of the frame 1115, and flows into the flow guide groove 1113 under the guidance of the liquid guide surface 1116, and then flows out through the slot 1114 of the flow guide groove 1113.

[0128] The liquid guide surface 1116 can be an inclined plane or an inclined curved surface, as long as it can guide the liquid downwards and outwards.

[0129] In other embodiments, the slide support 1112 can also not be provided with the liquid guide surface 1116.

[0130] In other embodiments, the liquid guide surface 1116 can also be vertically arranged to guide the liquid downwards into the flow guide groove 1113.

[0131] In the pathological staining operation, asynchronous operation is required. For example, in a hospital laboratory, a laboratory may need to detect several pathological tissue samples at the same time. However, these pathological tissue samples may come from different biological parts. Therefore, different samples need to be subjected to different operation steps, different reagents may be used in different steps, and different temperature control requirements may be required in different steps. In the related art, one placing table is integrated into one slide 90 placing position, and one placing table uses one set of heating system. The power of a single heating system is large. For the foregoing case, when the total amount of samples is small and asynchronous operation or different reagent adding is required, it may occur that only one slide 90 is placed on a placing platform with one placing position. Therefore, resources and space are wasted.

[0132] In order to make the slide placing heating device 10 be able to flexibly meet the needs of asynchronous operation and different reagent addition when applied to the pathological staining process, the slide placing heating device 10 is configured to include only one support part, so as to avoid waste. In other embodiments, the slide placing heating device 10 includes two or three or four support parts.

[0133] In order to provide more stable support to the slide 90 and ensure the heating effect on the slide 90, the slide stage 111 is configured in the following manner: the slide stage 111 includes a top plate 1111 and a slide holder 1112; the first communication port 11021 is provided on the top plate 1111, and the support part is provided on the slide holder 1112; the slide holder 1112 is provided on the side of the top plate 1111 away from the inner cavity 1101, and the top plate 1111 supports the slide holder 1112; the slide holder 1112 includes a frame body 1115 and a rib 1117, and the support part is the rib 1117 or the support part includes the rib 1117; the frame body 1115 includes oppositely arranged first and second frame parts, one end of the rib 1117 is connected with the first frame part, and the other side is connected with the second frame part; the ribs 1117 are arranged at intervals, and the third communication port 11023 is formed between adjacent ribs 1117, and the third communication port 11023 communicates with the inner cavity 1101 through the first communication port 11021. By supporting the main body part of the slide 90 with a plurality of ribs 1117, the stability of the placement of the slide 90 can be improved, and the reliability of the detection experiment can be ensured. Through the arrangement of the third communication port 11023, the area of the third communication port 11023 is large and the width of the rib 1117 is small, so that the hot air flow in the inner cavity 1101 can uniformly and effectively contact the slide 90 for heating, and the arrangement of the rib 1117 does not affect the heating effect.

[0134] The slide holder 1112 further includes a limiting structure 1118 for limiting the displacement of the slide 90 in the front-rear direction.

[0135] Embodiment three:

[0136] The technical solutions of the above-mentioned embodiment one or the technical solutions of the above-mentioned embodiment one and embodiment two are included. Please refer to Figures 11 to 14 In this embodiment, the first liquid adding module 22 realizes reagent addition by means of atomization spraying.

[0137] In the related art, reagent addition is generally realized by a dripping method, and the dripping method is that the reagent naturally drips on the slide 90, and there are problems of concentrated reagent addition position and large reagent consumption. When the reagent is added by the dripping method, the dropper is generally placed above the slide 90, and the liquid in the dropper is dropped by gravity to complete actual addition, but due to the viscosity and surface tension of the liquid, a certain amount of liquid needs to be dropped at a time, and if the amount is less than this amount, the liquid cannot be dropped and the reagent addition cannot be completed, therefore, the dripping method has a minimum addition liquid amount limit, and the addition requirement less than this liquid amount cannot be met, which easily causes waste of the reagent, and the dripping method also has the disadvantage of uneven reagent addition. In the related art, there are problems of uneven addition and large consumption in the process of immunohistochemical staining.

[0138] Please refer to Figure 13 、 Figure 14 The first liquid adding module 22 includes an atomizing sheet 222, the atomizing sheet 222 is a microporous atomizing sheet 222, the reagent is atomized into small water droplets by high-frequency vibration of the atomizing sheet 222, and then sprayed to the slide 90 or other positions, which can realize addition of a smaller liquid amount, the liquid spraying amount is controllable, the reagent can be saved, the liquid coverage area is larger and more uniform, and the reagent contacts the sample more uniformly.

[0139] Exemplarily, the minimum liquid amount that can be added by the atomizing module at a time is about 1 microliter, and the reagent particles after atomization are 3 micrometers to 20 micrometers. When performing immunohistochemical examination, compared with the existing droplet method for adding reagents, the atomizing module of the present application can reduce the amount of liquid used compared with the existing droplet method for adding reagents, and can effectively save costs.

[0140] The atomizing sheet 222 is a piezoelectric microporous atomizing sheet 222, and the atomizing sheet 222 includes a piezoelectric ceramic sheet 2221 and a microporous sheet 2222. On this basis, the setting mode of the atomizing sheet 222 can be realized by any one of the following embodiments:

[0141] The atomizing sheet 222 setting mode one: the atomizing sheet 222 is a microporous atomizing sheet 222 with a leakage prevention function, the microporous sheet 2222 is a microporous atomizing film sheet, and the microporous atomizing film sheet is an elastic film sheet.

[0142] The atomizing sheet 222 setting mode two: the atomizing sheet 222 is a common piezoelectric ceramic atomizing sheet 222, the microporous sheet 2222 is a metal sheet, and the metal sheet is provided with a plurality of atomizing holes 2227.

[0143] The atomizing sheet 222 setting mode one will be described in detail as follows:

[0144] When the dyeing treatment device 20 is used to add reagent to the slide 90, the first liquid adding module 22 is located above the slide table 111, and the first liquid adding module 22 sprays the reagent downward to realize reagent addition. In order to improve the spraying efficiency, the atomizing sheet 222 is parallel to the slide 90 when the first liquid adding module 22 is used to spray the reagent downward. In this way, when the liquid amount above the atomizing sheet 222 is large, the reagent may seep downward through the atomizing holes 2227 of the atomizing sheet 222 under the action of gravity, and then the reagent seeps, which leads to insufficient reagent addition accuracy or pollution of the slide 90 or other positions.

[0145] In the related art, in order to reduce the reagent seepage amount, the number of the atomizing holes 2227 of the atomizing sheet 222 is generally reduced or the hole diameter of the atomizing holes 2227 is reduced. However, since the atomizing effect is related to the number of the atomizing holes 2227, reducing the number of the atomizing holes 2227 will reduce the atomizing efficiency and the atomizing effect of the atomizing sheet 222. If the hole diameter of the atomizing holes 2227 is reduced, the viscous liquid cannot be atomized or is difficult to be atomized, which leads to reduced atomizing effect and atomizing performance.

[0146] In order to improve or solve the problem of liquid seepage of the atomizing sheet 222, the atomizing sheet 222 is provided in the above-mentioned first mode, that is, the micro-porous sheet 2222 is a micro-porous membrane sheet with elastic deformation performance. When the first liquid adding module 22 is vertically placed and not working, the liquid receiving part 2224 of the micro-porous membrane sheet deforms under the action of liquid weight, so that the atomizing holes 2227 deform, thereby closing or reducing the hole diameter of the liquid inlet holes of the atomizing holes 2227, to prevent liquid seepage and reduce the liquid seepage amount, which can avoid reagent waste and improve reagent addition accuracy.

[0147] The micro-porous atomizing sheet 222 is a piezoelectric micro-porous atomizing sheet 222, and the atomizing sheet 222 includes a micro-porous membrane sheet and a piezoelectric ceramic sheet 2221. The micro-porous membrane sheet is provided with a plurality of atomizing holes 2227, and the micro-porous membrane sheet is connected with the piezoelectric ceramic sheet 2221. A high-frequency alternating voltage is provided by a driving power supply to make the piezoelectric ceramic sheet 2221 vibrate at high frequency, thereby driving the micro-porous membrane sheet to vibrate, so that the liquid passing through the atomizing holes 2227 is atomized. The micro-porous membrane sheet includes a liquid receiving part 2224 and a mounting part 2223, the mounting part 2223 is connected with the outer edge of the liquid receiving part 2224, and the mounting part 2223 is used to be connected with the piezoelectric ceramic sheet 2221.

[0148] The piezoelectric ceramic atomizing sheet 222 is provided with a through hole. The mounting portion 2223 of the micro-porous atomizing diaphragm is attached to the piezoelectric ceramic atomizing sheet 222, and the mounting portion 2223 is fixed relative to the piezoelectric ceramic atomizing sheet 222. The liquid receiving portion 2224 of the micro-porous atomizing diaphragm is aligned with the through hole of the piezoelectric ceramic atomizing sheet 222, the liquid receiving portion 2224 is exposed by the through hole, the liquid receiving portion 2224 is provided with a plurality of atomizing holes 2227, the liquid receiving portion 2224 is used to contact liquid, and the atomizing holes 2227 provide atomizing channels. When the piezoelectric ceramic atomizing sheet 222 is in operation, the piezoelectric ceramic sheet 2221 drives the micro-porous atomizing diaphragm to vibrate.

[0149] Please refer to Figure 11 、 Figure 12 In order to improve or avoid the situation that the liquid seeps downward through the atomizing holes 2227 when the atomizing device is vertically placed without reducing the number of atomizing holes 2227, an elastic diaphragm is used as the micro-porous atomizing diaphragm. The elastic diaphragm can deform under the action of force and can restore to the original shape after the external force is removed.

[0150] Since the micro-porous atomizing diaphragm is an elastic diaphragm, when the liquid receiving portion 2224 of the micro-porous atomizing diaphragm is subjected to a downward force, the liquid receiving portion 2224 of the micro-porous atomizing diaphragm deforms, the inlet deforms, and the area of the inlet decreases or the inlet is closed. When the area of the inlet decreases, the amount of liquid seepage decreases; when the inlet is closed, the liquid above the liquid receiving portion 2224 cannot seep downward through the atomizing holes 2227.

[0151] When the atomizing device is in a shutdown state and is vertically placed, the first mist outlet 2231 of the atomizing device faces downward. At this time, the micro-porous atomizing diaphragm is horizontal, and the gravity of the liquid in the liquid tank acts on the liquid receiving portion 2224. Since the micro-porous atomizing diaphragm is an elastic diaphragm, if the weight of the liquid in the liquid tank is large, the downward force acting on the liquid receiving portion 2224 is large, and the liquid receiving portion 2224 is concave downward under the action of the gravity of the liquid. At this time, the inlet deforms from a circular shape to an oval shape, the area of the inlet decreases, and the liquid seepage downward can be hindered. When the inlet continues to deform from an oval shape to a straight line shape, the inlet is closed, and the liquid seepage downward can be prevented. When the downward force acting on the liquid receiving portion 2224 decreases or disappears, the liquid receiving portion 2224 can restore to the initial state under the action of its own elasticity.

[0152] It should be noted that when the atomizing device is turned on, the micro-porous atomizing diaphragm vibrates under the action of the piezoelectric ceramic, the inlet can be opened, the liquid can enter the atomizing holes 2227, and the liquid can be sprayed out from the first mist outlet 2231.

[0153] The micro-porous atomizing diaphragm can be, but is not limited to, a polyimide diaphragm, a polytetrafluoroethylene diaphragm, a polyethylene diaphragm, a polyethylene terephthalate diaphragm, and a polyvinylidene fluoride diaphragm.

[0154] Please continue to refer to Figure 11 、 Figure 12 The liquid receiving portion 2224 also includes an edge region 2226 which does not have the atomizing holes 2227, and the edge region 2226 is arranged between the mounting portions 2223 in the atomizing region 2225. The micro-porous atomizing diaphragm is an elastic diaphragm, and the mounting portions 2223 are fixed relative to the piezoelectric ceramic sheet 2221, and the central region of the liquid receiving portion 2224 is more likely to deform relative to the region adjacent to the mounting portions 2223, and the atomizing holes 2227 are more likely to shrink and close. By arranging the edge region 2226, the shrinkage effect of the liquid inlet in the atomizing region 2225 can be ensured when the liquid acts on the liquid receiving portion 2224 by gravity.

[0155] In order to make the liquid inlet more likely to deform to a closed state when the liquid receiving portion 2224 is subjected to the action of the liquid weight, the atomizing holes 2227 are arranged in a plurality of rows in a linear arrangement. By arranging a plurality of linearly arranged atomizing holes 2227, the liquid inlet of the atomizing holes 2227 in the same row is more likely to deform along the center line of the row and then fold, so that the deformation of the liquid inlet of the atomizing holes 2227 tends to be stretched and folded. This deformation mode can reduce the pore size of the liquid inlet, and when the liquid inlet is completely folded, the liquid inlet is completely closed, thereby avoiding liquid leakage.

[0156] In order to make the liquid inlet have a tendency to deform in a specific manner under the action of the liquid weight on the liquid receiving portion 2224, on the basis of arranging the atomizing holes 2227 in a plurality of rows in a linear arrangement, the atomizing holes 2227 in the atomizing region 2225 are also arranged in an asymmetric design: in the atomizing region 2225, the number of atomizing holes 2227 in each row is a, and the number of rows in the atomizing region 2225 is b, and a is greater than b. In this way, the elastic modulus of the atomizing region 2225 in the x direction is less than the elastic modulus of the atomizing region 2225 in the y direction, that is, the atomizing region 2225 is more likely to stretch and deform in the x direction when the liquid weight acts on the upper surface.

[0157] Example Four:

[0158] The technical solutions of the above-mentioned embodiment one are included; or, on the basis of the above-mentioned embodiment one, one or two of the technical solutions of embodiments two to three are included.

[0159] The atomizing module of the embodiment uses a separate liquid tank, and the atomizing module is detachably mounted on the device main body 11. When this kind of atomizing module is used as a reagent adding device, it is convenient to replace the atomizing module according to the actual different to be added to meet the demand of adding different reagents, which is more flexible.

[0160] Different reagents or other liquids are contained in the independent liquid cavities 2211 of different atomization modules, and different adding and different dyeing requirements can be met by replacing the atomization modules. When the dyeing treatment device 20 is applied in a laboratory, different samples are sent to the laboratory, and different dyeing reagents need to be used for different samples. In the case that the atomization modules can be replaced, the same dyeing treatment device 20 can be used to replace the atomization modules storing different reagents according to different reagent adding requirements, which is high in efficiency, high in flexibility, and wide in application range.

[0161] Please refer to Figure 13 、 Figure 14 、 Figure 15 In an embodiment of the atomization module of the present application, the atomization module comprises a shell 221, a base 223, and an atomization sheet 222.

[0162] The inside of the shell 221 forms a liquid cavity 2211 for containing liquid to be added, such as reagents. The atomization sheet 222 is arranged between the shell 221 and the base 223, and the base 223 is connected with the shell 221 to realize the installation of the atomization sheet 222. The atomization sheet 222 comprises an atomization area 2225, and a plurality of atomization holes 2227 are arranged in the atomization area 2225. The atomization sheet 222 disperses the water molecule structure of the liquid by high-frequency resonance, and sprays water mist including a large number of fine water droplets through the atomization holes 2227 of the atomization sheet 222.

[0163] In order to realize the dispersion and spraying of the liquid, the shell 221 is provided with a first opening 2212 communicating with the liquid cavity 2211, the base 223 is provided with a first mist outlet 2231, one side of the atomization area 2225 is aligned with the first opening 2212 of the shell 221, so that the liquid in the liquid cavity 2211 can contact the atomization sheet 222, and the other side of the atomization area 2225 is aligned with the first mist outlet 2231 of the base 223, so that the liquid that has completed atomization through the atomization holes 2227 can be sprayed out of the atomization module through the first mist outlet 2231.

[0164] In this embodiment, the atomization module is a device for spraying the atomized liquid downward. The liquid cavity 2211, the atomization sheet 222, and the base 223 are arranged in sequence along the height direction of the atomization module, the atomization sheet 222 is arranged below the liquid cavity 2211, and the first mist outlet 2231 of the atomization module is arranged below the atomization module, so that the water mist liquid can be sprayed downward.

[0165] The atomization module of the present application adds reagents by the atomization spraying mode, the liquid sprayed by the atomization module is soft and will not damage the sample, the liquid has a wider and more uniform coverage area under the same liquid volume, and the minimum adding liquid volume of the atomization module is reduced, so that the reagent can be saved.

[0166] The atomization module of the present application has an independent liquid tank. When the atomization module is used as a reagent adding device, different atomization modules can be replaced according to the different types of reagents to meet the needs of adding different reagents, which is more flexible. For example, in the pathological staining operation, there is an asynchronous operation requirement. When the pathological section staining equipment is used, the steps to be performed and the reagents to be added on different glass slides 90 are different. The atomization module containing reagent one can be installed first, and then the atomization module containing reagent two can be installed. This can meet the needs of asynchronous addition and different types of addition, and is more flexible to use and has a wide range of applications.

[0167] In order to make the structure of the atomization module more simple, the shell 221 includes a bottom plate 113 part, and the first opening 2212 is arranged on the bottom plate 113 part. The first opening 2212 is arranged at the bottom of the shell 221. The liquid in the liquid cavity 2211 does not need to be pumped to the atomization sheet 222 by using a pump. The atomization module does not need to be provided with a liquid pump or other pressure device. The structure of the atomization module can be simplified. The liquid in the liquid cavity 2211 can flow to the atomization sheet 222 under the action of gravity. The atomization sheet 222 can spray outward after being electrified. The efficiency is higher.

[0168] In order to make the liquid in the liquid cavity 2211 be able to be sprayed efficiently in the case that the remaining liquid amount is small, and avoid waste caused by liquid residue, a guide surface 2213 is arranged in the liquid cavity 2211 for guiding the liquid at the bottom to the atomization sheet 222.

[0169] In this embodiment, the shell 221 is arranged in the following manner: the shell 221 includes a top plate 1111 part, a side plate 112 part and a bottom plate 113 part. The liquid cavity 2211 is surrounded by the top plate 1111 part, the side plate 112 part and the bottom plate 113 part. The first opening 2212 is arranged on the bottom plate 113 part. The side of the bottom plate 113 part close to the top plate 1111 part forms a guide surface 2213. The guide surface 2213 is a downwardly inclined surface. The guide surface 2213 can be an inclined plane or an inclined curved surface.

[0170] Embodiment five:

[0171] The technical solutions of the above-mentioned embodiment one are included. Or, on the basis of the above-mentioned embodiment one, one or more technical solutions in embodiments two to four are included.

[0172] In the staining step, high-value staining reagents such as primary antibodies and secondary antibodies need to be added. After the addition of the primary antibody reagent is completed, a period of incubation is required. The cleaning step is performed to avoid the mixing of the residual primary antibody reagent and the secondary antibody reagent. Then, the secondary antibody reagent is added. In the cleaning step, a cleaning liquid is used for cleaning. The cleaning liquid has a low cost compared with the staining reagent.

[0173] In the related art, the same liquid adding module is generally used to add different reagents such as dyeing reagents and cleaning reagents. However, due to different effects to be achieved in different steps, the same liquid adding module cannot meet different requirements.

[0174] Please refer to Figure 15 、 Figure 16 In this embodiment, the dyeing treatment device 20 includes a mounting body 21, a first liquid adding module 22, and a second liquid adding module. The mounting body 21 provides mounting positions for the two liquid adding modules, and the first liquid adding module 22 and the second liquid adding module are respectively mounted on the mounting body 21.

[0175] The first liquid adding module 22 is an atomization module, which is used to add liquid to the slide 90 by atomization spraying. The atomization module includes a first mist outlet 2231, which is used to spray mist after atomizing the liquid into many small water droplets.

[0176] The second liquid adding module includes a liquid adding pipe 23, a liquid pump, and a liquid storage container. The liquid adding pipe 23 includes a liquid outlet 231, the liquid pump is connected to the liquid adding pipe 23, and the liquid pump is used to pump the liquid in the liquid storage container to the liquid adding pipe 23 and send it out through the liquid outlet 231. The liquid adding pipe 23 is used to add liquid by ordinary liquid adding. The liquid adding pipe 23 adds liquid to the slide 90 by spraying liquid, and the force of the liquid spray can be adjusted to avoid damage to the tissue sample. When the dyeing treatment device 20 is applied to an immunohistochemical dyeing device, the liquid adding pipe 23 can be connected to the liquid storage container, and the liquid pump can be used to pump the liquid in the liquid storage container to the liquid adding pipe 23 and spray it out through the liquid outlet 231 of the liquid adding pipe 23.

[0177] The mounting body 21 includes a mounting plate, and the mounting plate is provided with a pipe mounting portion 2223, and the liquid adding pipe 23 is mounted on the pipe mounting portion 2223. In this embodiment, the pipe mounting portion 2223 is a groove.

[0178] The first mist outlet 2231 and the liquid outlet 231 are arranged on the same side of the dyeing device. The liquid outlet side of the dyeing treatment device 20 can be directed towards the slide 90 to which liquid is to be added, so that the atomization module or the liquid adding pipe 23 can be selected to add liquid to the slide 90 according to actual requirements.

[0179] It should be noted that the terms "liquid", "solution", and "reagent" in the present application are the same.

[0180] The dyeing treatment device 20 of the present application integrates two liquid adding modules in one device. The atomization module adds liquid by atomization spraying, and the liquid adding pipe 23 adds liquid by spraying. Using the atomization module to add liquid can reduce the minimum amount of liquid added, save liquid, and achieve more uniform and gentle addition of liquid. Using the liquid adding pipe 23 to add liquid has high efficiency and is suitable for use in the cleaning step. When the liquid adding pipe 23 is used to add reagent, it can provide a larger amount of liquid and facilitate better cleaning effect. The dyeing treatment device 20 integrates two liquid adding modules, which can be used in different steps to add liquid. This not only avoids wasting high-cost liquid, but also makes the use more flexible.

[0181] For example, the first liquid adding module 22 can be used to uniformly add high-cost reagents such as primary antibody reagent and secondary antibody reagent by atomization spraying. The second liquid adding module can be used to add ordinary reagents such as cleaning agent by adding liquid through the liquid adding pipe 23.

[0182] In order to improve the cleaning efficiency of the cleaning step, the dyeing treatment device 20 is provided with a blowing module 24. The blowing module 24 is installed on the installation main body 21 and is used to blow air flow to the slide 90. The liquid adding pipe 23 cooperates with the blowing module 24. In the cleaning step, the liquid adding pipe 23 is used to add cleaning liquid to the slide 90, and the blowing module 24 is used to blow air flow to the slide 90. The air flow blows the liquid on the slide 90 in a specific direction, which produces the effect of wind scraping the cleaning liquid on the slide 90. A small amount of cleaning liquid can also be used to clean thoroughly, improving the cleaning efficiency. The liquid adding pipe 23 and the blowing module 24 are integrated in the same dyeing treatment device 20. They can be driven by a single moving module to move synchronously, achieving the effect of directional liquid and wind scraping cleaning. This kind of directional liquid and wind scraping cleaning method can also avoid the residue of primary antibody reagent or cleaning liquid, and avoid diluting the concentration of secondary antibody reagent.

[0183] The first mist outlet 2231, the air outlet 241 and the liquid outlet 231 are formed on the same side of the dyeing treatment device 20. Without the need to adjust the posture of the dyeing treatment device 20, it is convenient to add liquid to the slide 90 and to blow air to the slide 90 to use the air flow to scrape the liquid on the slide 90.

[0184] In the related art, the cleaning scheme in the pathological tissue dyeing treatment process is water bath solution cleaning or siphon cleaning. Using water bath method to clean wastes a lot of solution reagent, and using siphon method to clean cannot clean thoroughly. The present application uses the liquid adding pipe 23 to cooperate with the blowing module 24 to move synchronously, achieving the effect of directional liquid flow and wind flow scraping. This method can not only save reagent, but also clean thoroughly.

[0185] To provide better cleaning results through the second liquid addition module, the liquid addition tube 23 is tilted relative to the horizontal plane parallel to the slide 90. Tiltping the liquid addition tube 23 prevents it from being perpendicular to the slide 90 when adding liquid, thus reducing the impact force of the liquid on the sample on the slide 90 and minimizing damage to the sample tissue.

[0186] Example 6:

[0187] The technical solution includes the above-described embodiment one; or, based on the above-described embodiment one, it includes one or more technical solutions from embodiment two to embodiment five.

[0188] Please refer to Figure 1 , Figure 2 The moving device 30 includes a frame and a drive unit 313. The frame is a gantry.

[0189] The frame includes a first frame 311 and a second frame 312. The main body 11 of the slide placement heating device 10 is installed on the first frame 311. The staining treatment device 20 is movably connected to the second frame 312. The second frame 312 includes an x-axis guide rail 3122 and a y-axis guide rail 3121. The driving device 313 drives the staining treatment device 20 to move along the x-axis guide rail 3122 or the y-axis guide rail 3121.

[0190] The second frame 312 includes a fixed frame and a movable arm. The fixed frame is equipped with a Y-axis guide rail 3121, and the movable arm is also equipped with a Y-axis guide rail 3121. The first frame 311 is mounted on the fixed frame. The movable arm is slidably mounted on the Y-axis guide rail 3121 via a first pulley assembly, and the dyeing treatment device 20 is slidably mounted on the X-axis guide rail 3122 via a second pulley assembly.

[0191] The driving device 313 includes a first linear driving device 313 and a second linear driving device 313. The first linear driving device 313 is used to drive the moving arm and dyeing treatment device 20 to reciprocate in the y direction, and the second linear driving device 313 is used to drive the dyeing treatment device 20 to reciprocate in the x direction.

[0192] The glass slide placement and heating device 10 installed on the first frame 311 can be the structure in Embodiment 2, or it can be another structure.

[0193] Example 7:

[0194] To facilitate understanding of the pathological slide staining device of the present invention, a method for staining pathological slide samples based on the pathological slide staining device of the present invention is provided:

[0195] Place the sample after slicing on a glass slide 90;

[0196] The slide 90 is placed on the slide placing heating device 10;

[0197] The heating device 12 and the blowing device 13 in the temperature adjusting assembly are started in the baking step, the temperature in the inner cavity 1101 is monitored in real time by the temperature detector, and the power of the heating device 12 and the blowing device 13 is adjusted to control the temperature in the inner cavity 1101 of the device main body 11 within the target temperature;

[0198] The moving device 30 controls the dyeing treatment device 20 to move forward, backward, left and right so that the dyeing treatment device 20 moves to the top of the slide 90 first;

[0199] In the dewaxing and hydration step, the liquid adding pipe 23 of the second liquid adding module adds room temperature gradient alcohol solution and / or other reagents to the sample of the slide 90;

[0200] In the antigen repair step, the liquid adding pipe 23 of the second liquid adding module adds repair solution to the sample of the slide 90, and the temperature adjusting assembly controls the temperature in the inner cavity 1101 within the target temperature;

[0201] In the dyeing step, the first liquid adding module 22 adds primary reagent to the sample of the slide 90 by atomizing spraying, and enters the incubation stage. If necessary, after the addition of the primary reagent and the incubation are completed, the second liquid adding module 22 adds secondary reagent to the sample of the slide 90;

[0202] In the cleaning step, the liquid adding pipe 23 of the second liquid adding module adds cleaning solution to the sample of the slide 90, the blowing module 24 blows air to the slide 90, the air power is controllable and adjustable, and the moving device 30 controls the dyeing treatment device 20 to move from front to back or from left to right to realize directional liquid flow and blowing scraping to clean;

[0203] In the re-dyeing step, the first liquid adding module 22 adds constant-temperature DAB reagent to the sample of the slide 90 by atomizing spraying.

[0204] In the dewaxing and hydration step and the antigen repair step, if necessary, the first liquid adding module 22 can also be used to add liquid.

[0205] In the above reagent adding process, the excess reagent can be discharged from the slide 90 to the flow guide groove 1113. In the cleaning step, the cleaning solution is scraped to the direction of the flow guide groove 1113 by the moving and blowing of the dyeing treatment device 20. In this way, the cooperation of the slide placing heating device 10, the dyeing treatment device 20 and the moving device 30 can also achieve good removal and recovery of excess reagents, keep the slide 90 clean, and avoid the influence of reagent residues on the detection experiment results.

[0206] It should be noted that the above pathological section staining process can be increased, reduced, adjusted according to actual situation, the above steps are not as a limit of the present application.

[0207] In the description of the present application, it should be understood that the terms "upper", "lower", "left", "right" and other orientation or position relations are based on the orientation or position relations shown in the drawings, and are only for the convenience of description and simplification of operation, and do not indicate or imply that the devices or elements referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore cannot be understood as a limitation of the present application. In addition, the terms "first", "second" are only used to distinguish in the description, and have no special meaning.

[0208] In the description of the present application, the description of the terms "an embodiment", "an example" and the like means that the specific features, structures, materials or characteristics described in conjunction with the embodiment or example are contained in at least one embodiment or example of the present application. In the present application, the illustrative description of the above terms does not necessarily refer to the same embodiment or example.

[0209] In addition, it should be understood that although the present application is described in terms of embodiments, each embodiment does not contain only one independent technical solution, and the description of the present application is only for the sake of clarity, and those skilled in the art should consider the present application as a whole, and the technical solutions in each embodiment can be appropriately combined to form other embodiments which can be understood by those skilled in the art.

[0210] The technical principles of the present application are described above in conjunction with specific embodiments. These descriptions are only for the purpose of explaining the principles of the present application, and cannot be interpreted in any way as a limitation of the scope of protection of the present application. Based on the explanation here, those skilled in the art can think of other specific embodiments of the present application without creative labor, and these embodiments will fall within the scope of protection of the present application.

Claims

1. A pathological section staining device, characterized in that, include: A glass slide placement heating device (10) includes a device body (11) and a temperature regulating component. The device body (11) includes a slide stage (111) which provides a position for placing a glass slide (90). The temperature regulating component is used to heat and / or cool the glass slide (90). The staining apparatus (20) includes a mounting body (21) and a first liquid addition module (22) mounted on the mounting body (21); the first liquid addition module (22) is used to add reagents to a glass slide (90); A moving device (30) is used to move the position of the staining device (20) and / or the slide placement heating device (10) so that the staining device (20) moves relative to the slide placement heating device (10); The dyeing treatment device (20) includes a second liquid addition module, which is installed on the mounting body (21); the first liquid addition module (22) is an atomizing module, which includes a first mist outlet (2231) and is used to atomize and spray liquid; the second liquid addition module includes a liquid addition pipe (23) and a liquid pump, which is connected to the liquid addition pipe (23) and the liquid addition pipe (23) includes a liquid outlet (231); The atomizing module adds liquid by atomizing spraying, and the liquid adding pipe (23) adds liquid by spraying. The atomizing module is detachably installed on the main body (11) of the device, and the liquid chambers (2211) of different atomizing modules are filled with different reagents or other liquids; The device body (11) has an inner cavity (1101) inside, the slide stage (111) includes a support portion, the support portion provides a position for placing the glass slide (90); the temperature regulating assembly includes a blowing device (13), the blowing device (13) is used to blow airflow into the inner cavity (1101); The slide placement heating device (10) includes a guide plate (14) connected to the device body (11); a second flow space (1105) is formed between the guide plate (14) and the device body (11), or the guide plate (14) is provided with a second flow space (1105); the device body (11) is provided with a flow port (1104), and the second flow space (1105) is connected to the inner cavity (1101) through the flow port (1104); the guide plate (14) is used to guide the gas in the inner cavity (1101) to the side of the slide stage (111) away from the inner cavity (1101); The slide stage (111) includes a flow guide groove (1113) disposed on the side of the support portion, and the slide stage (111) is provided with one or more of the flow guide grooves (1113); the end of the slide stage (111) forms the opening (1114) of the flow guide groove (1113), and the opening (1114) allows the fluid in the flow guide groove (1113) to flow out.

2. The pathological section staining device according to claim 1, characterized in that, The slide stage (111) is provided with a first communication port (11021) communicating with the inner cavity (1101); The temperature regulating assembly includes a heater (12); the heater (12) is disposed in the inner cavity (1101), and a first flow space (1103) is provided between the heater (12) and the support.

3. The pathological section staining device according to claim 2, characterized in that, The blowing device (13) is a crossflow fan.

4. The pathological section staining device according to claim 1, characterized in that, The first liquid addition module (22) is an atomizing device, which includes an atomizing plate (222), which is a microporous atomizing plate (222). The atomizing device includes a first mist outlet (2231). The atomizing device atomizes the liquid through the atomizing plate (222) and sprays it out through the first mist outlet (2231).

5. The pathological section staining device according to claim 1, characterized in that, The first liquid addition module (22) is an atomizing module, and the atomizing module is detachably connected to the main body (11) of the device; The atomizing module includes a housing (221), a base (223), and an atomizing plate (222); the atomizing plate (222) is disposed between the housing (221) and the base (223); The housing (221) has a liquid cavity (2211) inside, and the housing (221) has a first opening (2212) communicating with the liquid cavity (2211); the base (223) is connected to the housing (221); the base (223) has a first mist outlet (2231); The atomizing plate (222) includes an atomizing area (2225), which is provided with a plurality of atomizing holes (2227); one side of the atomizing area (2225) is aligned with the first opening (2212), and the other side is aligned with the first mist outlet (2231).

6. The pathological section staining device according to claim 1, characterized in that, The first mist outlet (2231) and the liquid outlet (231) are located on the same side of the dyeing treatment device (20).

7. The pathological section staining device according to claim 6, characterized in that, The dyeing treatment device (20) includes a blowing module (24); the blowing module (24) is installed on the mounting body (21); the blowing module (24) is provided with an air outlet (241), and the blowing module (24) is used to blow air out through the air outlet (241); the first mist outlet (2231), the liquid outlet (231) and the air outlet (241) are formed on the same side of the dyeing treatment device (20).

8. The pathological section staining apparatus according to any one of claims 1-7, characterized in that, The mobile device (30) includes a frame and a drive unit (313); The frame includes a first frame (311) and a second frame (312). The main body (11) of the slide placement heating device (10) is installed on the first frame (311). The staining treatment device (20) is movably connected to the second frame (312). The second frame (312) includes an x-axis guide rail (3122) and a y-axis guide rail (3121). The driving device (313) drives the staining treatment device (20) to move along the x-axis guide rail (3122) or the y-axis guide rail (3121).

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