A reaction cup signal acquisition method and device with a fault-tolerant mechanism

Abstract

The application relates to the technical field of in-vitro diagnosis equipment, in particular to a reaction cup signal collection method and device with a fault-tolerant mechanism. The collection device comprises a reaction cup rack, reaction cups arranged in a circumferential direction on the reaction cup rack, a reaction disc fixedly installed below the reaction cup rack to fix the reaction cup rack, an optical signal collection device arranged on the reaction disc, and a photoelectric encoder arranged below the reaction disc. The application adopts a single-reaction-cup multi-position high-speed signal collection and data analysis method, can screen out unqualified reaction cups, can identify abnormal reaction liquid in a reaction process, and effectively improves the reliability of detection results.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of in-vitro diagnostic equipment, in particular to a reaction cup signal acquisition method with a fault-tolerant mechanism. BACKGROUND

[0002] The reaction system of a conventional in-vitro diagnostic equipment (such as a full-automatic biochemical analyzer, a chemiluminescence analyzer, or a coagulation analyzer) adopts a structure of a rotating reaction disc, a plurality of transparent reaction cups are mounted on a reaction disc cup holder in a whole circle, and each reaction cup can simultaneously contain a reaction solution for in-vitro diagnosis. The reaction cup is generally located in a reaction disc groove with a constant temperature function. The inner side and the outer side of a certain position of the groove are generally provided with a light signal acquisition device and a light source, and the light signal acquisition device has the functions of I / V conversion and A / D conversion of the light signal.

[0003] The conventional reaction cup disc adopts a gear type code disc for positioning, so that the number of slits of the gear type code disc is the same as the number of the cuvettes. When the motor works to periodically rotate the reaction disc, the theoretical center point position of each reaction cup is sensed by a position sensor on the gear type code disc, the outer light source is sequentially irradiated on the center position of each reaction cup on the reaction disc cup holder, the light signal acquisition module can receive the light signal of each reaction cup, and the position of each reaction cup can be recognized by a reaction cup positioning device (an optical encoder or a groove type code disc) to trigger the light signal acquisition module to perform single-point low-speed sampling, and the sampled signal is sent to an upper computer for analysis.

[0004] The reaction cup is generally composed of plastic with good light transmission and needs to be reused. Due to scratches, spots, concave-convex points and other unfavorable conditions that cannot be avoided during the manufacturing, transportation and repeated use of the reaction cup, the local light transmission of the reaction cup is deteriorated, which affects the accurate acquisition of the light signal. When there are scratches and other unfavorable conditions in the reaction cup or there are bubbles in the reaction solution, the conventional single-point low-speed sampling mode will lead to incomplete detection, so that the detection result is unstable and inaccurate. SUMMARY

[0005] In order to overcome the shortcomings of the prior art, the purpose of the present application is to provide a reaction cup signal acquisition method and device with a fault-tolerant mechanism, which can perform multi-point sampling on the reaction cup and improve the usability and fault tolerance of the reaction cup.

[0006] To achieve the above-mentioned purpose, the present application provides the following technical scheme:

[0007] A reaction cup signal acquisition method with a fault-tolerant mechanism, comprising the following steps:

[0008] S10: washing the reaction cup, filling each reaction cup with water, turning on the light source, driving the reaction disc to rotate, and rotating the optical encoder with the driving mechanism;

[0009] S20: The light source is irradiated on the grating disc of the rotating photoelectric encoder, the photosensitive element of the photoelectric encoder receives the light signal, the light signal acquisition module acquires 1-m positions of the Nth reaction cup and the corresponding signal values ADC_N(0) to ADC_N(m-1) of each position, and the light signal acquisition module transmits the signal values to the microprocessor;

[0010] S30: The microprocessor analyzes the signal values of ADC_N(0) to ADC_N(m-1), analyzes whether the signal values jump in a unit time, and if there is no jump, it indicates that the reaction cup is intact; if there is a jump, the signal values of multiple positions of the waveform are analyzed whether they are greater than the threshold value, if the signal values of multiple positions are all less than the threshold value, it indicates that the reaction cup has a large scratch, the microprocessor reports the reaction cup abnormality to the upper computer, and the upper computer rejects the reaction cup; if the signal values of multiple positions have a value greater than the threshold value, the position with the highest signal value is selected from the 1-m positions and stored in the memory of the microprocessor, and the sampling value of the position is used as the detection value of the reaction liquid;

[0011] S40: The water in the reaction cup is pumped out, dried, and the reaction liquid is added, and the full-range light signal of the qualified reaction cup is collected and transmitted to the microprocessor, and the microprocessor analyzes whether the full-range light signal of each detected reaction cup is normal;

[0012] S50: If the full-range light signal jumps, the reaction liquid has a bubble abnormality, and the microprocessor reports the abnormality to the upper computer; the upper computer does not use the detection result of the reaction cup and prompts the user to re-detect the sample or automatically re-tests the sample.

[0013] As a preferred scheme of the reaction cup signal acquisition method with a fault-tolerant mechanism, the driving mechanism and the photoelectric encoder are coaxially connected and rotated.

[0014] As a preferred scheme of the reaction cup signal acquisition method with a fault-tolerant mechanism, in step S20, the position of the reaction cup is collected according to the change of light flux, when the light flux decreases to a constant value, it indicates that the light source has completely passed through the Nth reaction cup, the photosensitive element transmits the signal to the microprocessor, and the light signal acquisition module collects data; when the light flux rises, it indicates that the light signal starts to partially leave the Nth reaction cup, the photosensitive element transmits the signal to the microprocessor, and the light signal acquisition module stops data collection.

[0015] As a preferred scheme of the reaction cup signal acquisition method with a fault-tolerant mechanism, in step S50, the full-range light signal is the signal in the whole movement process from when the light signal completely passes through the Nth reaction cup to when the light signal starts to partially leave the Nth reaction cup.

[0016] As a preferred scheme of the reaction cup signal acquisition method with the fault-tolerant mechanism, in the step S50, the signal value of the jump of the full light signal is ADCi = ADC_N(i) - ADC_N(i+1), and when the change value of ADCi is greater than a threshold ref or ADCi / ADC_N(i) is greater than a threshold Aref, the reaction liquid has a bubble abnormality.

[0017] As a preferred scheme of the reaction cup signal acquisition method with the fault-tolerant mechanism, the microprocessor is a signal control chip, and the microprocessor includes a cleaning module and a water injection module, which are used for automatically cleaning the reaction cup and automatically injecting water into the reaction cup.

[0018] As a preferred scheme of the reaction cup signal acquisition method with the fault-tolerant mechanism, the light signal acquisition module adopts a high-speed A / D conversion chip.

[0019] A reaction cup signal acquisition device with a fault-tolerant mechanism includes a reaction cup rack, a plurality of reaction cups arranged in a circumferential direction on the reaction cup rack, a reaction disc arranged below the reaction cup rack and fixedly installed on the reaction cup rack, a light signal acquisition device arranged on the reaction disc, and a photoelectric encoder arranged below the reaction disc.

[0020] As a preferred scheme of the reaction cup signal acquisition method with the fault-tolerant mechanism, the photoelectric encoder is provided with a grating disc, one side of the grating disc is provided with a light source, and the other side of the grating disc is provided with a light-sensitive element for receiving light.

[0021] As a preferred scheme of the reaction cup signal acquisition method with the fault-tolerant mechanism, the acquisition device further includes a driving mechanism connected with the reaction disc and the photoelectric encoder, the driving mechanism is connected with a microprocessor, and the microprocessor is connected with an upper computer.

[0022] Compared with the prior art, the present application has the following beneficial effects: by adopting the high-speed signal acquisition and data analysis method of multiple positions of a single reaction cup, the present application can screen out unqualified reaction cups, identify abnormal reaction liquids during the reaction process, and effectively improve the reliability of the detection result. The present application can be applied to quality screening after production of the reaction cup, full-process data monitoring of the reaction process, and can also improve the usability and fault tolerance of the reaction cup. BRIEF DESCRIPTION OF DRAWINGS

[0023] In order to more clearly illustrate the technical solutions in the specific embodiments of the present application or the prior art, the drawings required to be used in the description of the specific embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are some embodiments of the present application, and other drawings can be obtained by those skilled in the art without creative effort on the basis of these drawings. Among them:

[0024] Figure 1 It is an exploded view of the explosion structure of the collecting device of the present application.

[0025] Figure 2 It is a schematic view of the overall structure of the collecting device of the present application.

[0026] Figure 3 It is a schematic view of the cross-sectional structure of the reaction cup of the present application.

[0027] Figure 4 It is a flowchart of the present application.

[0028] Figure 5 It is the light signal collected by the same reaction cup multiple times when the reaction cup has good light transmission.

[0029] Figure 6 It is a schematic view of the light signal collected when the reaction cup has scratches and spots. Figure 1 ;

[0030] Figure 7 It is a schematic view of the light signal collected when the reaction cup has multiple scratches and spots.

[0031] Figure 8 It is a schematic view of the light signal collected when the reaction cup has scratches and spots. Figure 2 ;

[0032] Figure 9 It is a schematic view of the light signal collected when the reaction cup has bubbles. Figure 1 ;

[0033] Figure 10 It is a schematic view of the light signal collected when the reaction cup has bubbles. Figure 2 .

[0034] In the figure, the reference numerals are: 1, reaction cup holder; 2, reaction cup; 3, reaction disc; 4, groove; 5, light signal collecting device; 6, photoelectric encoder. DETAILED DESCRIPTION

[0035] The preferred embodiments of the present application will be described below in conjunction with the drawings, and it should be understood that the preferred embodiments described herein are only used to illustrate and explain the present application, and are not used to limit the present application.

[0036] Example 1

[0037] Reference Figures 1-10 For the first embodiment of the present application, the reaction cup signal acquisition method with fault-tolerant mechanism is provided. By using the high-speed signal acquisition and data analysis method of multiple positions of a single reaction cup, unqualified reaction cups can be screened, and abnormal reaction liquid can be identified during the reaction process, thereby effectively improving the reliability of the detection result. The usability and fault tolerance of the reaction cup are improved.

[0038] Specifically, the method comprises the following steps: S10: the instrument is turned on, the upper computer sends an instruction to the microprocessor to control the cleaning module to clean the reaction cup, and then controls the injection module to fill each reaction cup with water; the light source is turned on, the upper computer sends an instruction to the microprocessor to start the driving mechanism to drive the reaction disc to rotate, and the photoelectric encoder rotates with the driving mechanism;

[0039] S20: the light source irradiates the grating disc of the rotating photoelectric encoder, the photosensitive element of the photoelectric encoder receives the light signal, when the light flux decreases to a constant value, it indicates that the light source has completely passed through the Nth reaction cup, the photosensitive element transmits the signal to the microprocessor, and the light signal acquisition module collects data; when the light flux rises, it indicates that the light signal starts to partially leave the Nth reaction cup, the photosensitive element transmits the signal to the microprocessor, and the light signal acquisition module stops data collection, that is, the 1-m positions of the Nth reaction cup are collected, and the signal value of each position is ADC_N(0) to ADC_N(m-1); the light signal acquisition module transmits the signal value to the microprocessor;

[0040] S30: the analysis module of the microprocessor analyzes the signal values of ADC_N(0) to ADC_N(m-1), analyzes whether the signal value jumps in a unit time, and if there is no jump, it indicates that the reaction cup is perfect; if there is a jump, the signal values of multiple positions of the waveform are analyzed whether they are greater than the threshold value, if the signal values of multiple positions are all less than the threshold value, it indicates that the reaction cup has a large scratch, the microprocessor reports the reaction cup to the upper computer, the upper computer removes the reaction cup, the instrument will not use the reaction cup for sample and reagent injection, and prompts the user to replace the reaction cup; if the signal values of multiple positions have a value greater than the threshold value, select a position Pos_Ni (the position where the highest signal value is located) without a scratch in the 1-m positions, and store it in the memory of the microprocessor, and use the sampling value of the position as the detection value of the reaction liquid sample;

[0041] S40: The host computer sends instructions to the microprocessor to control the cleaning module to pump out the water in the reaction cup and dry it, and sends instructions to the microprocessor to control the injection module to add the reaction solution, the microprocessor controls the optical signal acquisition module to collect the full-path optical signal of the qualified reaction cup, and after the full-path optical signal collection is completed, it is transmitted to the microprocessor, and the analysis module of the microprocessor analyzes the full-path optical signal of each detected reaction cup.

[0042] S50: If the full-path optical signal appears signal value jump (ΔADCi = ADC_N(i)-ADC_N(i+1), ΔADCi change value is greater than threshold ref or ΔADCi / ADC_N(i) is greater than threshold Δref), it is considered that the reaction solution has bubbles and other abnormalities; the microprocessor will report the abnormality to the host computer; the host computer will not use the detection result of the reaction cup and prompt the user to retest the sample or automatically retest the sample, avoiding the reporting of abnormal results.

[0043] Preferably, the driving mechanism is coaxially connected with the photoelectric encoder.

[0044] Preferably, in step S50, the full-path optical signal is the signal during the whole movement of the optical signal from starting to pass through the Nth reaction cup to starting to partially leave the Nth reaction cup.

[0045] Preferably, the optical signal acquisition module uses a high-speed A / D conversion chip.

[0046] Referring to Figures 1-3 A reaction cup signal acquisition device with fault tolerance mechanism, comprising a reaction cup holder 1, a plurality of reaction cup positions circumferentially arranged on the reaction cup holder 1, a reaction cup 2 arranged in each reaction cup position, a reaction disc 3 fixedly installed below the reaction cup holder 1 to fix the reaction cup holder 1, an optical signal acquisition device 5 arranged on the reaction disc 3, and a photoelectric encoder 6 arranged below the reaction disc 3; the reaction disc 3 is provided with a groove 4 corresponding to the reaction cup 2 for placing the reaction cup 2. The reaction disc 3 and the photoelectric encoder 6 are connected with a driving mechanism, the driving mechanism is connected with a microprocessor, and the microprocessor is connected with a host computer; the optical signal acquisition device 5 is connected with the microprocessor to transmit the collected signal to the microprocessor.

[0047] As Figure 3 shown, point A on the reaction cup represents the starting point of the optical signal starting to pass through the Nth reaction cup; point B represents the point at which the optical signal starts to partially leave the Nth reaction cup; that is, the position of 1-m collected is between A and B, and the collected optical signal waveform is as shown in Figure 5 The microprocessor analyzes whether the waveform has a mutation.

[0048] Preferably, the grating disc of the photoelectric encoder is engraved with a plurality of light-transmissive slits, the number of the slits is the same as the number of the reaction cup positions, the reaction disc is above the photoelectric encoder, and the non-light-transmissive lines above the photoelectric encoder correspond to the reaction cup positions. One side of the grating disc is a light source, and the other side is a light-sensitive element receiving light. The rotation of the grating disc with the driving mechanism causes the light beam passing through the grating disc to be interrupted, and through the receiving of the light-sensitive element and the processing of the electronic circuit, a specific electrical signal output is generated. The photoelectric code disc of the photoelectric encoder is coaxial with the motor, and when the motor rotates, the grating disc rotates at the same speed as the motor, the light flux received by the light-sensitive element changes synchronously with the light-transmissive lines, and the output waveform of the light-sensitive element becomes a pulse signal after shaping. According to the change of the pulse, the position of the reaction cup can be accurately understood.

[0049] In use, first, the instrument is turned on, the reaction cup is cleaned, each reaction cup is filled with water, the light source is turned on, the driving mechanism drives the reaction disc to rotate, and the light source is sequentially irradiated on each reaction cup; the light signal acquisition module acquires the full-range light signal of each reaction cup; each reaction cup has a certain width, and there is a certain time when the reaction cup passes in front of the sensor of the light signal acquisition module, and the light signal uses a high-speed A / D conversion chip. According to the position information fed back by the photoelectric encoder, 1 to m positions of the Nth reaction cup are acquired, and each position signal value is ADC_N(0) to ADC_N(m-1).

[0050] As shown in Figure 5 , the light signals of the same reaction cup collected multiple times are good in light transmission, the light transmission rate is lowest when the light source irradiates on the cup wall, the light transmission rate gradually increases with the rotation of the reaction disc, the signal is in the rising state, the light transmission rate is stable when the light source is completely away from the cup wall position at the center position, and the signal is in the horizontal state. The area irradiated on the cup wall is larger and larger, the light transmission rate becomes low, and the signal is in the falling state. When the reaction cup has scratches and other unfavorable conditions, the light transmission rate is low, and the signal falls, as shown in Figure 6 , Figure 7 or Figure 8As shown, the waveforms are shown when there are scratches or spots in the reaction cup. When there are scratches, analyze whether the signal values at multiple positions are greater than the threshold value. If the signal values at multiple positions are all less than the threshold value, it indicates that there is a large scratch in the reaction cup, and the signal control chip (MCU or FPGA) reports to the upper computer that the reaction cup is abnormal. The upper computer will exclude the reaction cup, the instrument will not use the reaction cup for sample and reagent injection, and the user will be prompted to replace the reaction cup. If the signal values at multiple positions are greater than the threshold value, select a position Pos_Ni (the position of the highest signal value) without scratches from 1-m positions and store it in the memory. Use the sampling value of the position as the detection value of the reaction liquid. By analyzing the signal values ADC_N(0) to ADC_N(m-1), if the reaction cup itself does not have scratches and other adverse conditions, it can be used normally. The water in the reaction cup is pumped out, dried, and the reaction liquid is added. The full light signal of the qualified reaction cup is collected, and whether the full light signal of each detected reaction cup is normal is analyzed. As shown in Figure 9 and Figure 10 If the full light signal has a jump in signal value (ΔADCi = ADC_N(i) - ADC_N(i+1), the change value ΔADCi is greater than the threshold value ref or ΔADCi / ADC_N(i) is greater than the threshold value Δref), it is considered that the reaction liquid has bubbles and other abnormalities. The signal control chip (MCU or FPGA) will report the abnormality to the upper computer. The upper computer will not use the detection result of the reaction cup and will prompt the user to retest the sample or automatically retest the sample, avoiding the reporting of abnormal results.

[0051] Referring to Figure 4For the specific flowchart of the present application, first, the instrument is turned on and the reaction cup is cleaned, the reaction cup is filled with water, the light source is turned on, the reaction disc is moved, the full-range light signal of each reaction cup is collected by the light signal collection module, and is converted into an electric signal, and the signal values ADC_N(0) to ADC_N(m-1) are analyzed to determine whether a jump occurs, and if there is no jump, it indicates that the reaction cup does not have a scratch; if there is a jump, the signal values ADC_N(0) to ADC_N(m-1) are analyzed to determine whether they are all less than the threshold value, which indicates that the reaction cup has a large scratch, and the microprocessor reports the reaction cup anomaly to the upper computer, the upper computer excludes the reaction cup, and the instrument will not use the reaction cup for sample and reagent filling; if the signal values of multiple positions are greater than the threshold value, a position Pos_Ni (the position of the highest signal value) that does not have a scratch is selected from 1-m positions and is stored in the memory of the microprocessor, and the position is used as the position for detecting the reaction liquid sample in the future; the water in the reaction cup is discharged, and the reaction liquid is added; then the qualified reaction cup is subjected to full-range light signal collection and conversion into an electric signal, and the full-range light signal is analyzed to determine whether a jump occurs, and if a jump occurs, the upper computer is reported and the reaction liquid is prompted to be replaced; if there is no jump, the detection result is used. By using the high-speed signal collection and data analysis method of multiple positions of the reaction cup, the present application can screen unqualified reaction cups, identify abnormal reaction liquids during the reaction process, and effectively improve the reliability of the detection result. It can also be applied to quality screening after reaction cup production, full-range data monitoring of the reaction process, and can also improve the usability and fault tolerance of the reaction cup.

[0052] Finally, it should be noted that: the above embodiments are only used to illustrate the technical solutions of the present application, and not to limit them; although the present application has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the foregoing embodiments, or make equivalent replacements for part or all of the technical features. Any modification, equivalent replacement, improvement, etc. made within the spirit and principles of the present application shall be included in the protection scope of the present application.

Claims

1. A reaction cup signal acquisition method with a fault-tolerant mechanism, applying a reaction cup signal acquisition device with a fault-tolerant mechanism, characterized in that: The reaction cup signal acquisition device comprises a reaction cup rack (1), a plurality of reaction cups (2) arranged circumferentially on the reaction cup rack (1), a reaction disc (3) arranged below the reaction cup rack (1) and fixedly installed on the reaction cup rack (1), a light signal acquisition device (5) arranged on the reaction disc (3), and a photoelectric encoder (6) arranged below the reaction disc (3). The signal acquisition method comprises the following steps: S10: The reaction cup is cleaned, each reaction cup is filled with water, a light source is turned on, and the driving mechanism drives the reaction disc to rotate, and the photoelectric encoder rotates with the driving mechanism; S20: The light source irradiates on the grating disc of the rotating photoelectric encoder, the photosensitive element of the photoelectric encoder receives the light signal, the light signal acquisition module acquires 1-m positions of the Nth reaction cup and the signal values ADC_N(0) to ADC_N(m-1) corresponding to each position, and the light signal acquisition module transmits the signal values to the microprocessor; S30: The microprocessor analyzes the signal values of ADC_N(0) to ADC_N(m-1), analyzes whether the signal values jump in a unit time, and if no jump occurs, it indicates that the reaction cup is intact; if there is a jump, the signal values of multiple positions of the waveform are analyzed whether they are greater than a threshold value, if the signal values of multiple positions are all less than the threshold value, it indicates that the reaction cup has a large scratch, the microprocessor reports the reaction cup abnormality to the upper computer, and the upper computer excludes the reaction cup; if the signal values of multiple positions are greater than the threshold value, the position with the highest signal value is selected from the 1-m positions and stored in the memory of the microprocessor, and the sampling value of the position is used as the detection value of the reaction liquid; S40: The water in the reaction cup is pumped out, dried, and the reaction liquid is added, the full light signal of the qualified reaction cup is acquired, and the full light signal is transmitted to the microprocessor, and the microprocessor analyzes whether the full light signal of each detected reaction cup is normal; S50: If the full light signal jumps, the reaction liquid has a bubble abnormality, the microprocessor reports the abnormality to the upper computer, and the upper computer does not use the detection result of the reaction cup and prompts the user to re-detect the sample or automatically re-tests the sample; In the step S20, the position of the reaction cup is acquired according to the change of light flux, when the light flux decreases to a constant value, it indicates that the light source completely passes through the Nth reaction cup, the photosensitive element transmits the signal to the microprocessor, and the light signal acquisition module acquires data; when the light flux rises, it indicates that the light signal starts to partially leave the Nth reaction cup, the photosensitive element transmits the signal to the microprocessor, and the light signal acquisition module stops data acquisition; In the step S50, the full light signal is the signal in the whole movement process from when the light signal completely passes through the Nth reaction cup to when the light signal starts to partially leave the Nth reaction cup; in the step S50, the signal value of the full light signal jump is ΔADCi = ADC_N(i)-ADC_N(i+1), when the change value of ΔADCi is greater than a threshold value ref or ΔADCi / ADC_N(i) is greater than a threshold value Δref, the reaction liquid has a bubble abnormality.

2. The reaction cup signal acquisition method with fault-tolerant mechanism according to claim 1, characterized in that: The driving mechanism is coaxially connected with the photoelectric encoder.

3. The reaction cup signal acquisition method with fault-tolerant mechanism according to claim 2, characterized in that: The microprocessor is a signal control chip, and the microprocessor includes a cleaning module and a water injection module, which are used for automatically cleaning the reaction cup and automatically injecting water into the reaction cup.

4. The reaction cup signal acquisition method with fault-tolerant mechanism according to claim 3, characterized in that: The light signal acquisition module adopts a high-speed A / D conversion chip.

5. The reaction cup signal acquisition method with fault-tolerant mechanism according to claim 1, characterized in that: The photoelectric encoder (6) is provided with a grating disc, one side of the grating disc is provided with a light source, and the other side of the grating disc is provided with a light-sensitive element for receiving light.

6. The reaction cup signal acquisition method with fault-tolerant mechanism according to claim 5, characterized in that: The driving mechanism is connected with the reaction disc (3) and the photoelectric encoder (6), the driving mechanism is connected with a microprocessor, and the microprocessor is connected with an upper computer.

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