A recombinant microorganism for simultaneously producing sesquiterpenes and primary metabolites and its application

Abstract

The present invention provides a recombinant microorganism for simultaneously producing sesquiterpenes and primary metabolites, wherein the primary metabolites include at least one of pantothenic acid, squalene, fatty acids and vitamin B2; the strain contains the mevalonate pathway, the β-farnesene synthesis gene and genes related to the synthesis of primary metabolites; the genes related to the mevalonate pathway include ERG10, ERG13, tHMG1, ERG12, ERG8, MVD1, IDI1 and ERG20 genes, and the copy numbers are ERG10:ERG13:tHMG1:ERG12:ERG8:MVD1:IDI1:ERG20 = 2:2:X:2:2:2:2:2, where X is an integer greater than or equal to 1; the β-farnesene synthesis gene includes bFS, and the sequence is as shown in SEQ ID NO.1. The present invention realizes high yield of primary metabolites while producing sesquiterpene compounds.

Description

Technical Field

[0001] The present invention relates to the field of bioengineering technology, and particularly relates to a recombinant microorganism capable of simultaneously producing sesquiterpenes and primary metabolites and its application. Background Art

[0002] Sesquiterpene compounds are a class of terpene compounds composed of three isoprene units, which are widely distributed in plants, microorganisms, insects, and various marine organisms. With the development of microbial technology, researchers have achieved the efficient synthesis of some sesquiterpene compounds in microorganisms. Sesquiterpene compounds are volatile products, and in their biosynthesis process, two-way extraction fermentation is often used, so that the target products will accumulate in the oil layer outside the cells. In the industrial production process, collecting the oil layer can achieve the collection of the target sesquiterpene compounds, while the bacterial liquid and bacteria will be directly discarded, which will generate a large amount of industrial waste. By producing intracellular or extracellular non-lipid-soluble products while producing extracellular products soluble in the oil phase, it is possible to rationally utilize the bacterial liquid and bacteria after fermentation, which is beneficial to green economy and energy-saving production in industry.

[0003] Primary metabolites refer to substances that are produced by microorganisms through metabolic activities and are essential for their own growth and reproduction, such as amino acids, nucleotides, polysaccharides, lipids, vitamins, pantothenic acid, squalene, and fatty acids. Through primary metabolism, nutrients can be converted into structural substances, physiologically active substances, or energy for growth. Therefore, primary metabolites are usually essential substances for the survival of the organism. As long as there is an obstacle in a certain link of the synthesis process of these substances, it will cause growth arrest at least, and lead to mutation or death of the organism at worst, which is a basic metabolic type.

[0004] In the prior art, for microorganisms that simultaneously produce sesquiterpenes and primary metabolites, due to possible competitive inhibition, it is difficult to achieve high yields of both.

[0005] Therefore, it is necessary to develop a recombinant microorganism that can simultaneously produce sesquiterpenes and primary metabolites with high yields. Summary of the Invention

[0006] The object of the present invention is to provide a recombinant microorganism capable of simultaneously producing sesquiterpenes and primary metabolites and its application, which can achieve high yields of primary metabolites while producing sesquiterpene compounds.

[0007] In the first aspect of the present invention, a strain capable of simultaneously producing sesquiterpenes and primary metabolites is provided. The strain contains the mevalonate pathway, some or all of the codon-optimized sesquiterpene synthesis genes, and genes related to the synthesis of primary metabolites; the primary metabolites include at least one of pantothenic acid, squalene, fatty acids, and vitamin B2; the sesquiterpenes include β-farnesene;

[0008] The related genes of the mevalonate pathway include ERG10, ERG13, tHMG1, ERG12, ERG8, MVD1, IDI1 and ERG20 genes; the copy numbers of the genes in the mevalonate pathway are ERG10:ERG13:tHMG1:ERG12:ERG8:MVD1:IDI1:ERG20 = 2:2:X:2:2:2:2:2, where X is an integer greater than or equal to 1;

[0009] The β-farnesene synthesis gene includes bFS, and the bFS gene contains mutations of F11S, M35T, T319S, I434T, I460V on the basis of the farnesene synthase from chamomile, and its nucleotide sequence is as shown in SEQ ID NO.1.

[0010] Furthermore, the related genes for pantothenic acid synthesis include ilv2, ilv6, ilv5, ilv3, Ecm31p, pan5, pan6 and PAND genes; the PAND gene has been codon-optimized, and its nucleotide sequence is as shown in SEQ ID NO.2.

[0011] Furthermore, the strains that simultaneously produce sesquiterpenes and the pantothenic acid include:

[0012] Strain JFS1 obtained by co-transforming plasmid pPT1, pPT2 and pXZ162 into Saccharomyces cerevisiae JVA139;

[0013] Or strain JFS2 obtained by knocking out the GAL80 gene in the strain JFS1;

[0014] Or strain JFS3 obtained by co-transforming plasmid pPT1, pPT3 and pXZ162 into Saccharomyces cerevisiae JVA139;

[0015] Or strain JFS4 obtained by knocking out the GAL80 gene in the strain JFS3;

[0016] Among them,

[0017] The plasmid pPT1 uses pMD19T as the backbone vector, the promoters are pGAL1, pGAL2, pGAL7 and pGAL10, and it contains ilv2, ilv6, ilv5 and ilv3 genes. The nucleotide sequence of the plasmid pPT1 is as shown in SEQ ID NO.3;

[0018] The plasmid pPT2 uses pMD19T as the backbone vector, the promoters are pGAL1, pGAL2, pGAL7 and pGAL10, and it contains Ecm31p, pan5, pan6 and PAND genes. The nucleotide sequence of the plasmid pPT2 is as shown in SEQ ID NO.4;

[0019] The plasmid pPT3 uses pMD19T as the backbone vector, with promoters pGAL1, pGAL7, and pGAL10, and contains the Ecm31p, pan5, and pan6 genes. The nucleotide sequence of the plasmid pPT3 is as shown in SEQ ID NO.5;

[0020] The nucleotide sequence of the plasmid pXZ162 is as shown in SEQ ID NO.6.

[0021] Furthermore, the genes related to squalene synthesis include the POS5, ADH2, ACS2, ALD6, and ERG9 genes.

[0022] Furthermore, the strains that simultaneously produce sesquiterpenes and the squalene include:

[0023] The strain JSX1 obtained by co-transforming the linearized plasmids pXZ171, pXZ159, and pXZ161 into Saccharomyces cerevisiae JVA139; then the strain JSX2 obtained by co-transforming the linearized plasmid pSX01 and pXZ162 into the strain JSX1;

[0024] Or the strain JSX3 obtained by knocking out the GAL80 gene in the strain JSX2;

[0025] Among them,

[0026] The plasmid pXZ171 uses pMD19T as the backbone vector, with promoters pGAL1, pGAL7, and pGAL10, and contains the ADH2, ACS2, and ALD6 genes. The nucleotide sequence of the plasmid pXZ171 is as shown in SEQ ID NO.7;

[0027] The plasmid pXZ159 uses pMD19T as the backbone vector, with promoter pGAL1, and contains the POS5 gene. The nucleotide sequence of the plasmid pXZ159 is as shown in SEQ ID NO.8;

[0028] The plasmid pSX01 uses pMD19T as the backbone vector, with promoter pHXT1, and contains the ERG9 gene. The nucleotide sequence of the plasmid pSX01 is as shown in SEQ ID NO.9;

[0029] The nucleotide sequence of the plasmid pXZ161 is as shown in SEQ ID NO.10.

[0030] Furthermore, the genes related to fatty acid synthesis include the ACC1, FAS1, and FAS2 genes, and the glucose-inducible promoter pHXT1 and the truncated signal peptide thiolase tesA from Escherichia coli are used.

[0031] Furthermore, the strains for simultaneously producing sesquiterpenes and the fatty acids include:

[0032] Strain JZF01 obtained by co-transforming linearized plasmids pZF01, pZF02, and pZF03 and pZF04 into Saccharomyces cerevisiae JVA139;

[0033] Or strain JZF02 obtained by knocking out the GAL80 gene in the strain JZF01;

[0034] Wherein,

[0035] The plasmid pZF01 uses pMD19T as the backbone vector, the promoter is pHXT1, and it contains the tesA gene. The nucleotide sequence of the plasmid pZF01 is as shown in SEQ ID NO.11;

[0036] The plasmid pZF02 uses pMD19T as the backbone vector, the promoter is pHXT1, and it contains the ACC1 gene. The nucleotide sequence of the plasmid pZF02 is as shown in SEQ ID NO.12;

[0037] The plasmid pZF03 uses pMD19T as the backbone vector, the promoter is pHXT1, and it contains the FAS1 and FAS2 genes. The nucleotide sequence of the plasmid pZF03 is as shown in SEQ ID NO.13;

[0038] The nucleotide sequence of the plasmid pZF04 is as shown in SEQ ID NO.14.

[0039] Furthermore, the genes related to vitamin B2 synthesis include the RIB1, RIB7, RIB2, RIB3, RIB4, and RIB5 genes.

[0040] Furthermore, the strains for simultaneously producing sesquiterpenes and vitamin B2 include:

[0041] Strain JVB01 obtained by co-transforming linearized plasmids pVB1 and pVB2 and pXZ162 into Saccharomyces cerevisiae JVA139;

[0042] Or strain JVB02 obtained by knocking out the GAL80 gene in the strain JVB01;

[0043] Wherein,

[0044] The plasmid pVB1 uses pMD19T as the backbone vector, the promoters are pGAL1, pGAL7, and pGAL10, and it contains the RIB1, RIB7, and RIB2 genes. The nucleotide sequence of the plasmid pVB1 is as shown in SEQ ID NO.15;

[0045] The plasmid pVB2 uses pMD19T as the backbone vector, with promoters pGAL1, pGAL7, and pGAL10, and contains the RIB3, RIB4, and RIB5 genes. The nucleotide sequence of the plasmid pVB2 is shown in SEQ ID NO.16;

[0046] The nucleotide sequence of the plasmid pXZ162 is shown in SEQ ID NO.6.

[0047] In the second aspect of the present invention, there is provided the use of the bacterium that simultaneously produces sesquiterpenes and primary metabolites in the production of sesquiterpenes and primary metabolites. The seed liquid of the strain is inoculated into a culture medium for eukaryotic expression to obtain sesquiterpenes and primary metabolites.

[0048] One or more technical solutions in the embodiments of the present invention have at least the following technical effects or advantages:

[0049] The recombinant microorganism capable of simultaneously producing sesquiterpenes and primary metabolites and its application provided by the present invention can achieve high yields of primary metabolites while producing sesquiterpene compounds. Among them, the recombinant microorganism strengthens the mevalonate pathway, and the copy numbers of genes in the mevalonate pathway are ERG10:ERG13:tHMG1:ERG12:ERG8:MVD1:IDI1:ERG20 = 2:2:X:2:2:2:2:2, where X is an integer greater than or equal to 1. Among them, ERG10 is the gene encoding acetoacetyl-CoA thiolase, ERG13 is the gene encoding HMG-CoA synthase, tHMG1 is the gene encoding HMG-CoA reductase, ERG12 is the gene encoding mevalonate kinase, ERG8 is the gene encoding mevalonate-5-phosphate kinase, MVD1 is the gene encoding mevalonate pyrophosphate decarboxylase, IDI1 is the gene encoding isopentenyl pyrophosphate isomerase, and ERG20 is the gene encoding farnesyl pyrophosphate synthase. The farnesene synthesis gene bFS is introduced into the strain to achieve high yields of farnesene. On the basis of the strain with high yields of farnesene, the high yields of primary metabolites squalene or vitamin B2 or pantothenic acid or fatty acids in the strain are further enhanced. BRIEF DESCRIPTION OF THE DRAWINGS

[0050] In order to more clearly illustrate the technical solutions in the embodiments of the present invention, the following will briefly introduce the drawings required for the description of the embodiments. Obviously, the drawings in the following description are some embodiments of the present invention. For those of ordinary skill in the art, without creative efforts, other drawings can also be obtained based on these drawings.

[0051] Figure 1 It is the map of plasmid pPT1;

[0052] Figure 2 It is the map of plasmid pPT2;

[0053] Figure 3 It is the map of plasmid pPT3;

[0054] Figure 4 It is the map of plasmid pXZ162;

[0055] Figure 5 It is the map of plasmid pXZ171;

[0056] Figure 6 It is the map of plasmid pXZ159;

[0057] Figure 7 It is the map of plasmid pXZ161;

[0058] Figure 8 It is the map of plasmid pSX01;

[0059] Figure 9 It is the map of plasmid pZF01;

[0060] Figure 10 It is the map of plasmid pZF02;

[0061] Figure 11 It is the map of plasmid pZF03;

[0062] Figure 12 It is the map of plasmid pZF04;

[0063] Figure 13 It is the map of plasmid pVB1;

[0064] Figure 14 It is the map of plasmid pVB2;

[0065] Figure 15 It is the map of plasmid pZY600;

[0066] Figure 16 It is the map of plasmid pZY413;

[0067] Figure 17 It is the map of plasmid pZY412L;

[0068] Figure 18 It is the map of plasmid pZY414;

[0069] Figure 19 It is the map of plasmid pZY900;

[0070] Figure 20 It is the map of plasmid pBFS45. Detailed implementation manners

[0071] The present invention will be specifically described below in conjunction with specific embodiments and examples, and the advantages and various effects of the present invention will be presented more clearly therefrom. Those skilled in the art should understand that these specific embodiments and examples are for illustrating the present invention, rather than limiting the present invention.

[0072] Throughout the specification, unless otherwise specifically stated, the terms used herein should be understood as having the meanings commonly used in the art. Therefore, unless otherwise defined, all technical and scientific terms used herein have the same meaning as the general understanding of those skilled in the art to which the present invention pertains. In case of any contradiction, this specification shall prevail.

[0073] Unless otherwise specifically stated, various raw materials, reagents, instruments, and equipment used in the present invention can be obtained through market purchases or by existing methods.

[0074] The technical solutions of the embodiments of the present application for solving the above technical problems are generally as follows:

[0075] According to a typical embodiment of the present invention, a strain for simultaneously producing sesquiterpenes and primary metabolites is provided.

[0076] The strain contains genes related to the mevalonate pathway, some or all of the codon-optimized sesquiterpene synthesis genes, and genes related to the synthesis of primary metabolites; the primary metabolites include at least one of pantothenic acid, squalene, fatty acids, and vitamin B2; the sesquiterpenes include β-farnesene.

[0077] The genes related to the mevalonate pathway include ERG10, ERG13, tHMG1, ERG12, ERG8, MVD1, IDI1, and ERG20 genes; the copy numbers of the genes in the mevalonate pathway are ERG10: ERG13: tHMG1: ERG12: ERG8: MVD1: IDI1: ERG20 = 2: 2: X: 2: 2: 2: 2: 2, where X is an integer greater than or equal to 1.

[0078] The β-farnesene synthesis gene includes bFS, and the bFS gene contains mutations of F11S, M35T, T319S, I434T, and I460V based on the farnesene synthase from chamomile, and its nucleotide sequence is as shown in SEQ ID NO.1.

[0079] The Accession / GENE id of ERG10, ERG13, tHMG1, ERG12, ERG8, MVD1, IDI1, and ERG20 in NCBI is shown in Table 1 below.

[0080] Table 1 - Genes related to the mevalonate pathway

[0081] Gene Full name Accession / GENE id ERG10 Gene of acetoacetyl-CoA thiolase 856079 ERG13 Gene of HMG-CoA synthase 854913 tHMG1 Gene of HMG-CoA reductase 854900, truncated at 4 - 1659bp ERG12 Gene of mevalonate kinase NM_001182715.1 ERG8 Gene of mevalonate-5-phosphate kinase CP046093.1, 689693..691048 MVD1 Gene of mevalonate pyrophosphate decarboxylase NM_001183220.1 IDI1 Gene of isopentenyl diphosphate isomerase NM_001183931.1 ERG20 Gene of farnesyl diphosphate synthase 853272

[0082] In the recombinant microorganism of the embodiment of the present invention, the mevalonate pathway is strengthened, and the copy numbers of genes in the mevalonate pathway are ERG10:ERG13:tHMG1:ERG12:ERG8:MVD1:IDI1:ERG20 = 2:2:X:2:2:2:2:2, where X is an integer greater than or equal to 1. Among them, ERG10 is the gene encoding acetoacetyl-CoA thiolase, ERG13 is the gene encoding HMG-CoA synthase, tHMG1 is the gene encoding HMG-CoA reductase, ERG12 is the gene encoding mevalonate kinase, ERG8 is the gene encoding mevalonate-5-phosphate kinase, MVD1 is the gene encoding mevalonate pyrophosphate decarboxylase, IDI1 is the gene encoding isopentenyl pyrophosphate isomerase, and ERG20 is the gene encoding farnesene pyrophosphate synthase.

[0083] The reason for the above ERG10:ERG13:tHMG1:ERG12:ERG8:MVD1:IDI1:ERG20 = 2:2:X:2:2:2:2:2, where X is an integer greater than or equal to 1: The above copy number ratio can strengthen the mevalonate pathway, so as to provide the maximum precursor supply;

[0084] The farnesene synthesis gene bFS is introduced into the strain. The bFS gene contains mutations of F11S, M35T, T319S, I434T, and I460V on the basis of the farnesene synthase derived from chamomile, and its nucleotide sequence is as shown in SEQ ID NO.1. After the mutation, high-yield production of farnesene can be achieved.

[0085] At the same time, on the basis of the strain with high-yield farnesene, the high-yield production of at least one of the primary metabolites pantothenic acid, squalene, fatty acid, and vitamin B2 in the strain is further strengthened.

[0086] As a specific implementation manner, the strain for simultaneously producing sesquiterpenes and primary metabolites is the strain for simultaneously producing sesquiterpenes and the above pantothenic acid; among them,

[0087] The genes related to pantothenic acid synthesis include ilv2, ilv6, ilv5, ilv3, Ecm31p, pan5, pan6, and PAND gene; the PAND gene has been codon-optimized, and its nucleotide sequence is as shown in SEQ ID NO.2.

[0088] The strain for simultaneously producing sesquiterpenes and the above pantothenic acid includes:

[0089] The strain JFS1 obtained by co-transforming plasmids pPT1, pPT2, and pXZ162 into Saccharomyces cerevisiae JVA139;

[0090] Or the strain JFS2 obtained by knocking out the GAL80 gene in the strain JFS1;

[0091] Or the strain JFS3 obtained by co-transforming the plasmids pPT1, pPT3, and pXZ162 into the Saccharomyces cerevisiae JVA139;

[0092] Or the strain JFS4 obtained by knocking out the GAL80 gene in the strain JFS3;

[0093] Wherein,

[0094] As Figure 1 shown, the plasmid pPT1 uses pMD19T as the backbone vector, the promoters are pGAL1, pGAL2, pGAL7, and pGAL10, and it contains the ilv2, ilv6, ilv5, and ilv3 genes. The nucleotide sequence of the plasmid pPT1 is as shown in SEQ ID NO.3;

[0095] As Figure 2 shown, the plasmid pPT2 uses pMD19T as the backbone vector, the promoters are pGAL1, pGAL2, pGAL7, and pGAL10, and it contains Ecm31p, pan5, pan6, and PAND genes. The nucleotide sequence of the plasmid pPT2 is as shown in SEQ ID NO.4;

[0096] As Figure 3 shown, the plasmid pPT3 uses pMD19T as the backbone vector, the promoters are pGAL1, pGAL7, pGAL10, and it contains Ecm31p, pan5, and pan6 genes. The nucleotide sequence of the plasmid pPT3 is as shown in SEQ ID NO.5;

[0097] As Figure 4 shown, the nucleotide sequence of the plasmid pXZ162 is as shown in SEQ ID NO.6. The plasmid pXZ162 is a gRNA plasmid targeting the rDNA locus, which is used to guide the Cas9 protein to cut the target site to achieve the integration of the target gene into the target site.

[0098] Table 2 - Genes related to pantothenic acid synthesis

[0099]

[0100] In the above technical solution, the principles by which strains JFS1, JFS2, JFS3, and JFS4 can produce pantothenic acid and the reasons for their high yields: In wild-type Saccharomyces cerevisiae, which is a pantothenic acid-deficient strain and requires supplementation with pantothenic acid to maintain its normal growth, there has been no reported efficient synthesis of pantothenic acid in Saccharomyces cerevisiae yet. In the present invention, by overexpressing the rate-limiting enzyme in the pantothenic acid synthesis pathway (specifically shown in Table 2) and introducing the heterologous gene Aspartate 1-decarboxylase (PAND) synthesized from Escherichia coli after codon optimization in Saccharomyces cerevisiae to provide sufficient precursors, efficient synthesis of pantothenic acid can be achieved in Saccharomyces cerevisiae strains.

[0101] Among them, strains JFS2 and JFS4 can synthesize sesquiterpenes and produce high yields of target primary metabolites without the addition of galactose induction after knocking out their GAL80 genes, which can reduce the experimental process and fermentation costs.

[0102] As a specific embodiment, the strain that simultaneously produces sesquiterpenes and primary metabolites is the strain that simultaneously produces sesquiterpenes and the said squalene; among them,

[0103] The genes related to squalene synthesis include the POS5, ADH2, ACS2, ALD6, and ERG9 genes.

[0104] The strain that simultaneously produces sesquiterpenes and the said squalene includes:

[0105] Strain JSX1 obtained by co-transforming linearized plasmids pXZ171, pXZ159, and pXZ161 into Saccharomyces cerevisiae JVA139; then strain JSX2 obtained by co-transforming linearized plasmid pSX01 and pXZ162 into the said strain JSX1;

[0106] Or the strain JSX3 obtained by knocking out the GAL80 gene in the said strain JSX2;

[0107] Among them,

[0108] As Figure 5 shown, the plasmid pXZ171 uses pMD19T as the backbone vector, the promoters are pGAL1, pGAL7, and pGAL10, and it contains the ADH2, ACS2, and ALD6 genes. The nucleotide sequence of the plasmid pXZ171 is as shown in SEQ ID NO.7;

[0109] As Figure 6 shown, the plasmid pXZ159 uses pMD19T as the backbone vector, the promoter is pGAL1, and it contains the POS5 gene. The nucleotide sequence of the plasmid pXZ159 is as shown in SEQ ID NO.8;

[0110] As Figure 8 shown, the plasmid pSX01 uses pMD19T as the backbone vector, the promoter is pHXT1, contains the ERG9 gene, and the nucleotide sequence of the plasmid pSX01 is as shown in SEQ ID NO.9;

[0111] As Figure 7 shown, the nucleotide sequence of the plasmid pXZ161 is as shown in SEQ ID NO.10. The plasmid pXZ161 is a gRNA plasmid targeting the DPP1 and exg1 sites, used to guide the Cas9 protein to cleave the target site, and achieve the integration of the target gene into the target site.

[0112] Table 3 - Related genes for squalene synthesis

[0113] Gene Full name Accession / GENE id POS5 NADH kinase 855913 ADH2 Ethanol dehydrogenase 855349 ACS2 Acetyl-CoA synthetase 850846 ALD6 Acetaldehyde dehydrogenase 856044 ERG9 Squalene synthase 856597

[0114] In the above technical solution, the principles by which the strains JSX2 and JSX3 can produce squalene and the reasons for their high yields: In strains that efficiently synthesize farnesene, the synthesis precursor FPP of squalene will be competed, so it is difficult to achieve the simultaneous efficient synthesis of the two compounds. In the present invention, the supply of the common precursor acetyl-CoA of the MVA pathway is further strengthened, and the inducible promoter pHXT1 is used to control the overexpression of ERG9. Through temporal and spatial interleaving, squalene is efficiently synthesized in the early stage and farnesene is efficiently synthesized in the later stage, so that the efficient synthesis of the two compounds can be achieved;

[0115] Among them, since the GAL80 gene of the strain JSX3 is knocked out, after knocking out Gal80, the strain can synthesize sesquiterpenes and high-yield target primary metabolites without adding galactose induction, which can reduce the experimental process and fermentation cost.

[0116] As a specific implementation manner, the strain for simultaneously producing sesquiterpenes and primary metabolites is the strain for simultaneously producing sesquiterpenes and the fatty acid, wherein,

[0117] The related genes for fatty acid synthesis include the ACC1, FAS1, and FAS2 genes, and the glucose-inducible promoter pHXT1 and the truncated signal peptide thiolase tesA from Escherichia coli are used.

[0118] The strain for simultaneously producing sesquiterpenes and the fatty acid includes:

[0119] The strain JZF01 obtained by co-transforming the linearized plasmids pZF01, pZF02, and pZF03 and pZF04 into the Saccharomyces cerevisiae JVA139;

[0120] Alternatively, the strain JZF01 is subjected to knockout of the GAL80 gene to obtain the strain JZF02;

[0121] Among them,

[0122] As Figure 9 shown, the plasmid pZF01 uses pMD19T as the backbone vector, the promoter is pHXT1, and it contains the tesA gene. The nucleotide sequence of the plasmid pZF01 is as shown in SEQ ID NO.11;

[0123] As Figure 10 shown, the plasmid pZF02 uses pMD19T as the backbone vector, the promoter is pHXT1, and it contains the ACC1 gene. The nucleotide sequence of the plasmid pZF02 is as shown in SEQ ID NO.12;

[0124] As Figure 11 shown, the plasmid pZF03 uses pMD19T as the backbone vector, the promoter is pHXT1, and it contains the FAS1 and FAS2 genes. The nucleotide sequence of the plasmid pZF03 is as shown in SEQ ID NO.13;

[0125] As Figure 12 shown, the nucleotide sequence of the plasmid pZF04 is as shown in SEQ ID NO.14. The plasmid pZF04 is a gRNA plasmid targeting the POX1, FAA1, and FAA4 sites, and is used to guide the Cas9 protein to cleave the target site to achieve the integration of the target gene into the target site.

[0126] Table 4 - Genes related to fatty acid synthesis

[0127]

[0128]

[0129] In the above technical solution, the principles by which the strains JZF01 and JZF02 can produce fatty acids and the reasons for their high yields are as follows: in the strains for the efficient synthesis of farnesene, the synthesis precursor acetyl-CoA of fatty acids will be competed, so it is difficult to achieve the simultaneous efficient synthesis of the two compounds. In the present invention, the inducible promoter pHXT1 is used to control the overexpression of fatty acid synthesis genes (such as ACC1, FAS1, and FAS2 in Table 4). Through temporal and spatial interleaving, fatty acids are efficiently synthesized in the early stage and farnesene is efficiently synthesized in the later stage, so that the efficient synthesis of the two compounds can be achieved; at the same time, by knocking out the fatty acid degradation-related genes acyl-CoA synthetase FAA1, FAA4, and β-oxidase POX1 with the plasmid pZF04, the yield of fatty acids is further increased;

[0130] Among them, the strain JZF02 has its GAL80 gene knocked out. After knocking out Gal80, the strain can synthesize sesquiterpenes and produce high-yield target primary metabolites without adding galactose induction, which can reduce the experimental process and fermentation cost.

[0131] As a specific implementation manner, the strain for simultaneously producing sesquiterpenes and primary metabolites is the strain for simultaneously producing sesquiterpenes and the vitamin B2, wherein,

[0132] The genes related to vitamin B2 synthesis include RIB1, RIB7, RIB2, RIB3, RIB4 and RIB5 genes.

[0133] The strain for simultaneously producing sesquiterpenes and the vitamin B2 includes:

[0134] Strain JVB01 obtained by co-transforming linearized plasmid pVB1 and pVB2 with pXZ162 into Saccharomyces cerevisiae JVA139;

[0135] Or the strain JVB01 is knocked out for the GAL80 gene to obtain strain JVB02;

[0136] Among them,

[0137] As Figure 13 shown, the plasmid pVB1 uses pMD19T as the backbone vector, the promoters are pGAL1, pGAL7 and pGAL10, and it contains RIB1, RIB7 and RIB2 genes. The nucleotide sequence of the plasmid pVB1 is as shown in SEQ ID NO.15;

[0138] As Figure 14 shown, the plasmid pVB2 uses pMD19T as the backbone vector, the promoters are pGAL1, pGAL7 and pGAL10, and it contains RIB3, RIB4 and RIB5 genes. The nucleotide sequence of the plasmid pVB2 is as shown in SEQ ID NO.16;

[0139] As Figure 4 shown, the nucleotide sequence of the plasmid pXZ162 is as shown in SEQ ID NO.6. The plasmid pXZ162 is a gRNA plasmid targeting the rDNA locus, which is used to guide the Cas9 protein to cut the target site to achieve the integration of the target gene into the target site.

[0140] Table 5 - Genes related to vitamin B2 synthesis

[0141]

[0142]

[0143] In the above technical solution, the principles by which strains JVB01 and JVB02 can produce riboflavin and the reasons for their high yields: There has been no report on the efficient synthesis of riboflavin in Saccharomyces cerevisiae. In the present invention, by overexpressing the rate-limiting enzymes in the riboflavin synthesis pathway (as shown in Table 5), efficient synthesis of riboflavin can be achieved on the basis of strains that efficiently synthesize farnesene.

[0144] Among them, since strain JVB02 has its GAL80 gene knocked out, after knocking out Gal80, the strain can synthesize sesquiterpenes and highly produce target primary metabolites without the addition of galactose induction, which can reduce the experimental process and fermentation cost.

[0145] In other embodiments, the primary metabolites include 2-4 of pantothenic acid, squalene, fatty acids, and riboflavin, and microorganisms that simultaneously produce sesquiterpenes, pantothenic acid, squalene, fatty acids, and riboflavin may also be included within the scope of protection of the present invention.

[0146] Next, a recombinant microorganism that simultaneously produces sesquiterpenes and primary metabolites and its application according to the present application will be described in detail with reference to examples and experimental data.

[0147] Example 1. Construction of vectors required for chassis yeast strains

[0148] (1) Plasmid pZY600

[0149] The function of plasmid pZY600 is to integrate the Cas9 protein for subsequent strain modification using the Crispr-cas9 gene editing technology. The relevant characteristics of plasmid pZY600: △ChrXII-2: Hygr_pTEF1_Cas9_tCYC1 ( Figure 15 as shown), where pTEF1_Cas9_tCYC1 refers to the expression of Cas9 controlled by the TEF1 promoter, the terminator is cyc1, and the selection marker is Hygr, i.e., hygromycin; ΔchrXII-2 refers to the position of the expression cassette inserted into the chromosome.

[0150] Specific construction process of plasmid pZY600: (1) Using plasmid pZY403 (the construction process of said plasmid pZY403 is shown below) as a template, fragment G6001 (homologous left and right arms of ChrXII-2 site for integrating Cas9 gene, introducing NotI restriction site, plasmid backbone containing ampicillin resistance and ura tag) was obtained by PCR amplification with primers G6001-F and G6001-R; (2) Using pZY403 as a template, fragment G6002 (hygromycin resistance gene and its promoter TEF terminator TEF) was obtained by PCR amplification with primers G6002-F and G6002-R; (3) Using p43802 (addgene Plasmid#43802) as a template, fragment G6003 (gene Cas9 and its promoter TEF1 terminator CYC1) was obtained by PCR amplification with primers G6003-F and G6003-R. (3) The above fragments were recombinantly constructed into pZY600 in Saccharomyces cerevisiae by DNA assemble (yeast assembly), then amplified in Escherichia coli. After restriction enzyme digestion verification and correct sequencing, pZY600 was obtained. After NotI restriction enzyme digestion linearization, the fragment with the target gene was obtained, and its sequence is shown in SEQ ID NO.17.

[0151] Among them, the construction of the plasmid pZY403 is as follows: (1) Using the yeast 30000B genome as a template, fragment Z4031 (the homologous left arm of the ChrXII-2 site for integrating the ERG13 gene) was obtained by PCR amplification with primers Z4031-F and Z4031-R; (2) Using the yeast 30000B genome as a template, fragment Z4032 (promoter) was obtained by PCR amplification with primers Z4032-F and Z4032-R; (3) Using the yeast 30000B genome as a template, fragment Z4033 (ERG13 gene) was obtained by PCR amplification with primers Z4033-F and Z4033-R; (4) Using the yeast 30000B genome as a template, fragment Z4034 (terminator) was obtained by PCR amplification with primers Z4034-F and Z4034-R; (5) Using the plasmid pZY402 (see CN 111019850 A for details) as a template, fragment Z4035 (selection marker) was obtained by PCR amplification with primers Z4035-F and Z4035-R; (6) Using the yeast 30000B genome as a template, fragment Z4036 (the homologous right arm of the ChrXII-2 site for integrating the ERG13 gene) was obtained by PCR amplification with primers Z4036-F and Z4036-R; (7) Using pZY402 (see CN 111019850 A for details) as a template, fragment Z4037 (introducing a NotI restriction site, plasmid backbone containing ampicillin resistance and ura tag) was obtained by PCR amplification with primers Z4037-F and Z4037-R; (8) The above fragments were recombinantly constructed into pZY403 in Saccharomyces cerevisiae by the method of DNA assemble (yeast assembly), then amplified in Escherichia coli, and after digestion verification and correct sequencing, pZY403 was obtained.

[0152] The sequences of each primer used for constructing the above plasmid are shown in Table 6:

[0153] Table 6 - Primers required during the construction of plasmid pZY600

[0154]

[0155]

[0156] (2) Plasmid pZY413 and gRNA plasmid pZY607

[0157] The function of plasmid pZY413 is to overexpress the related genes in the MVA pathway, providing an efficient chassis strain for FPP precursor synthesis for subsequent gene screening. The related characteristics of plasmid pZY413: △ChrXI-3: ERG8_pGAL1pGAL10_tHMG1_pGAL7_ERG12( Figure 16) The genes ERG8, ERG12, and THMG1 were respectively controlled by the promoters GAL1, GAL7, and GAL10 for expression, and the inserted chromosomal locus was ChrXI-3.

[0158] Specific construction process of plasmid pZY413: (1) Using plasmid pZY402 (see CN 111019850 A for details) as a template, fragment 4131 (the homologous right arm for integrating the ERG8, tHMG1, and ERG12 genes, introducing a NotI restriction site and a part of the plasmid backbone containing ampicillin resistance and ura tag) was obtained by PCR amplification with primers 4131-F and 4121-R; (2) Using pZY402 (see CN 111019850 A for details) as a template, fragment 4132 (the remaining part of the plasmid backbone containing ampicillin resistance and ura tag, and a part of the homologous right arm of ChrXI-3 locus for integrating the ERG8, tHMG1, and ERG12 genes, introducing a NotI restriction site) was obtained by PCR amplification with primers 4122-F and 4132-R; (3) Using pZY402 as a template, fragment 4133 (the remaining part of the homologous right arm of ChrXI-3 locus for integrating the ERG8, tHMG1, and ERG12 genes, and a part of the target genes ERG8, tHMG1, ERG12 and their promoters and terminators) was obtained by PCR amplification with primers 4133-F and 4133-R; (4) Using pZY402 as a template, fragment 4134 (the remaining part of the target genes ERG8, tHMG1, ERG12 and their promoters and terminators) was obtained by PCR amplification with primers 4134-F and 4134-R. (5) The above fragments were recombinantly constructed into pZY413 in Saccharomyces cerevisiae by the DNA assemble method, then amplified in Escherichia coli. After correct enzyme digestion verification and sequencing, pZY413 was obtained. After NotI enzyme digestion and linearization, a fragment with the target gene was obtained, and its sequence is shown in SEQ ID NO.18.

[0159] The gRNA plasmid pZY607 is a gRNA plasmid targeting the ChrXI-3 locus and is a tool plasmid for targeting and cleaving genomic DNA of Saccharomyces cerevisiae in the Crispr-Cas9 gene editing technology. Based on the plasmid p43803 (http: / / www.addgene.org / 43803 / ), its guide-RNA sequence was replaced with atatgtctctaattttggaa. The specific construction process is as follows: (1) Using plasmid p43803 as a template, fragment 6071 (a 20bp target sequence for targeted recognition, gRNAscafold, terminator SUP4, and a part of the plasmid backbone containing ampicillin resistance and ura tag) was amplified by PCR with primers 6071-F and G6031-R; (2) Using p43803 as a template, fragment 6072 (the remaining part of the plasmid backbone containing ampicillin resistance and ura tag, a 20bp target sequence for targeted recognition, and promoter SNR52) was amplified by PCR with primers G6032-F and 6072-R. (3) The above fragments were recombinantly constructed into pZY607 in Saccharomyces cerevisiae by the method of DNAassemble.

[0160] The sequences of each primer used for constructing the above plasmids are shown in Table 7 below:

[0161] Table 7 - Primers required for the construction of plasmid pZY413 and gRNA plasmid pZY607

[0162]

[0163] (3) Plasmid pZY412L and gRNA plasmid pZY606

[0164] The function of plasmid pZY412L is to overexpress related genes in the MVA pathway and provide an efficient chassis strain for FPP precursor synthesis for subsequent gene screening. The relevant characteristics of plasmid pZY412L: △ChrXII-4: IDI1_pGAL1pGAL10_ERG10_pGAL7_MVD1( Figure 17 ), where the expression of genes IDI1, MVD1, and ERG10 is controlled by promoters GAL1, GAL7, and GAL10 respectively, and the inserted chromosomal locus is ChrXII-4.

[0165] Specific construction process of plasmid pZY412L: (1) Fragment pZY412 SPD (a meaningless DNA sequence used to replace the g418 resistance gene in pZY410 (see CN 111019850 A) and containing sticky ends with SalI and SpeI restriction sites) was obtained by PCR without template denaturation and annealing using primers 412-F and 412-R. The reaction system for this PCR without template denaturation and annealing was 10 μL, with 4.5 μL of each primer and 1 μL of 10×T4 DNA ligase buffer. The following PCR program was set: 95°C, 5 min; touchdown PCR, 95°C, 1 min, -1°C / cycle, 70 cycles; 25°C, 5 min; 12°C, ∞ (the fragment could be temporarily stored at -40°C after being taken out from the PCR without template denaturation and annealing). (2) After digesting pZY401 (see CN 111019850 A) with SalI and SpeI, a large fragment pZY412 VPD of 12,835 bp (a vector fragment containing the target genes MVD1, ERG10, IDI1, as well as ampicillin resistance and ura screening markers) was recovered by gel extraction. (3) Fragment pZY412 SPD and fragment pZY412 VPD were ligated overnight at 16°C (about 20 h) using T4 DNA ligase at a molar ratio of 3:1. The ligation product was transferred into competent Escherichia coli DH10B cells for amplification. After digestion verification and correct sequencing, plasmid pZY412L was obtained. After linearization with NotI, a fragment with the target gene was obtained, and its sequence is shown in SEQ ID NO.19.

[0166] gRNA plasmid pZY606 is a gRNA plasmid targeting the Chr XII-4 locus and is a tool plasmid for target recognition and cleavage of Saccharomyces cerevisiae genomic DNA in the Crispr-Cas9 gene editing technology. It replaced the guide-RNA sequence with gcttcaagaattgagtaaac based on plasmid p43803 (http: / / www.addgene.org / 43803 / ). The specific construction process is as follows: (1) Using plasmid p43803 as a template, fragment 6061 (a 20-bp target sequence for target recognition, gRNA scafold, terminator SUP4, and a part of the plasmid backbone containing ampicillin resistance and ura tags) was amplified by PCR using primers 6061-F and G6031-R; (2) Using p43803 as a template, fragment 6062 (the remaining part of the plasmid backbone containing ampicillin resistance and ura tags, a 20-bp target sequence for target recognition, and promoter SNR52) was amplified by PCR using primers G6032-F and 6062-R. (3) The above fragments were recombinantly constructed into pZY606 in Saccharomyces cerevisiae by the DNA assemble method.

[0167] The primer sequences used for constructing the above plasmids are shown in Table 8 below:

[0168] Table 8 - Primers required for the construction of plasmid pZY412L and gRNA plasmid pZY606

[0169]

[0170] (4) Plasmid pZY414 and gRNA plasmid pZY608

[0171] The function of plasmid pZY414 is to overexpress the relevant genes in the MVA pathway, providing an efficient chassis strain for FPP precursor synthesis for subsequent gene screening. The relevant characteristics of plasmid pZY414: △ChrX-3: pGAL1_ERG13_pGAL10_tHMG1( Figure 18 ), using promoters GAL1 and GAL10 to control the expression of genes ERG13 and THMG1 respectively, and the inserted chromosomal locus is ChrX-3.

[0172] The specific construction process of the plasmid pZY414: (1) Using the genome of Saccharomyces cerevisiae strain CEN.PK2-1D(30000B) as a template, fragment 4141 (the homologous right arm of ChrX-3 locus for integrating ERG13 and tHMG1 genes, introducing NotI restriction site) was obtained by PCR amplification with primers 4141-F and 4141-R; (2) Using plasmid pRS426(ATCC87333) as a template, fragment 4142 (the plasmid backbone containing ampicillin resistance and ura tag) was obtained by PCR amplification with primers 4142-F and 4142-R; (3) Using the 30000B genome as a template, fragment 4143 (the homologous right arm of ChrX-3 locus for integrating ERG13 and tHMG1 genes, introducing NotI restriction site) was obtained by PCR amplification with primers 4143-F and 4143-R; (4) Using pZY410 (see CN111019850 A for details) as a template, fragment 4144 (the target genes ERG13, tHMG1 and their promoters and terminators) was obtained by PCR amplification with primers 4144-F and 4144-R. (4) The above fragments were recombinantly constructed into pZY414 in Saccharomyces cerevisiae by DNA assemble method, then amplified in Escherichia coli, and after enzyme digestion verification and correct sequencing, the plasmid of pZY414 was obtained. After NotI digestion and linearization, the fragment with the target gene was obtained, and its sequence is shown in SEQ ID NO.20.

[0173] The gRNA plasmid pZY608 is a gRNA plasmid targeting the ChrX-3 locus and is a tool plasmid for identifying and cleaving the genomic DNA of Saccharomyces cerevisiae in the Crispr-Cas9 gene editing technology. On the basis of plasmid p43803, its guide-RNA sequence was replaced with ctaatgtgtccgcgtttcta. The specific construction process is as follows: (1) Using plasmid p43803 (http: / / www.addgene.org / 43803 / ) as a template, fragment 6081 (a 20bp target sequence for targeted recognition, gRNA scafold, terminator SUP4, and a part of the plasmid backbone containing ampicillin resistance and ura tag) was amplified by PCR using primers 6081-F and G6031-R; (2) Using p43803 as a template, fragment 6082 (the remaining part of the plasmid backbone containing ampicillin resistance and ura tag, a 20bp target sequence for targeted recognition, and promoter SNR52) was amplified by PCR using primers G6032-F and 6082-R. (3) The above fragments were recombinantly constructed into pZY608 in Saccharomyces cerevisiae by the method of DNA assemble, then amplified in Escherichia coli. After enzyme digestion verification and correct sequencing, pZY608 was obtained.

[0174] The sequences of each primer used for constructing the above plasmid are shown in Table 9 below:

[0175] Table 9 - Primers required for the construction of plasmid pZY414 and gRNA plasmid pZY608

[0176]

[0177] Example 2, Construction of chassis yeast strain

[0178] 1. The linearized fragment of the plasmid pZY600 obtained in Example 1 was transferred into a Saccharomyces cerevisiae strain (which can be Saccharomyces cerevisiae strains such as CEN.PK2-1D, CEN.PK2-1C, BY4742, BY4741, CEN.PK113-5D, etc. In this example of the present invention, CEN.PK2-1D was specifically selected), and integrated into the chromosome according to homologous recombination. The integration site was ChrXII-2, and the selection marker was hygromycin (Hygr). Thus, the background strain Saccharomyces cerevisiae JCR1 was successfully constructed.

[0179] 2. The linearized fragment of the plasmid pZY413 obtained in Example 1 and the gRNA plasmid pZY607 obtained in Example 1 were co-transformed into the Saccharomyces cerevisiae JCR1. The selection marker was uracil (URA3), and after counter-selection with 5-FOA to remove the plasmid, Saccharomyces cerevisiae JCR23 was obtained.

[0180] 3. The linearized fragment of the plasmid pZY414 obtained in Example 1 and the gRNA plasmid pZY608 obtained in Example 1 were co-transformed into the Saccharomyces cerevisiae JCR23. The selection marker was uracil (URA3). After removing the plasmid by 5-FOA counter-selection, the Saccharomyces cerevisiae JCR25 was obtained.

[0181] 4. The linearized fragment of the plasmid pZY412L obtained in Example 1 and the gRNA plasmid pZY606 obtained in Example 1 were co-transformed into the Saccharomyces cerevisiae JCR25. The selection marker was uracil (URA3). After removing the plasmid by 5-FOA counter-selection, the Saccharomyces cerevisiae strain JCR27 was obtained.

[0182] Table 10

[0183]

[0184] Example 3: Construction of the expression vector of farnesene synthase

[0185] 1. Plasmid pZY900

[0186] Relevant characteristics of plasmid pZY900: △LEU2: LEU2(URA3)_TCYC1_LacZ_pGAL10pGAL1_ERG20_tERG20( Figure 19 ), the expression of genes ERG20 and LacZ is controlled by the promoters GAL1 and GAL10 respectively. The selection marker is Leu2, and the inserted chromosomal locus is Leu2.

[0187] The specific construction process of the plasmid pZY900: (1) Using the yeast S288c genome as a template, fragments 9001 (left homologous arm of Leu2), 9002 (terminator tTDH2), 9006 (gene ERG20 and terminator tERG20), and 9007 (right arm of Leu2) were amplified by 900-1F / 1R, 900-2F / 2R, 900-6F / 6R, and 900-7F / 7R respectively; (2) Using the genome of yeast 30000B as a template, fragments 9003 (terminator tCYC1) and 9005 (promoters pGAL1 and Pgal10) were amplified by primers 900-3F / 3R and 900-5F / 5R respectively; (3) The plasmid backbone (introducing the MssI restriction site, selection marker) was amplified by primers 900-8F / 8R. (4) The above fragments were recombinantly constructed into pZY900 in Saccharomyces cerevisiae by the method of DNA assemble (yeast assembly), then amplified in Escherichia coli. After enzyme digestion verification and correct sequencing, pZY900 was obtained, and the sequence is shown in SEQ ID NO.11. The sequences of each primer used for constructing the above plasmid are shown in Table 11 below:

[0188] Table 11 - Primers required for the construction of plasmid pZY900

[0189]

[0190] 2. Plasmid pBFS45

[0191] Mac-bFS with mutations F11S, M35T, T319S, I434T, and I460V optimized according to the codons of Saccharomyces cerevisiae introduced mutations on the basis of the wild-type chamomile-derived farnesene synthase (Accession: AIG92847.1), and its nucleotide sequence is shown in SEQ ID NO.1;

[0192] The process of constructing plasmid pBFS1 (containing the wild-type farnesene synthase) is as follows: (1) Amplify the fragment from the nucleotide sequence of Mac-bFS using primers pBFS1-1F / pBFS1-1R. (2) Subsequently, clone it into pZY900 by the Golden Gate assembly method to obtain plasmid pBFS1.

[0193] The process of constructing plasmid pBFS45( Figure 20 ) is as follows: (1) Amplify the fragment from the nucleotide sequence of Mac-bFS with mutations F11S, M35T, T319S, I434T, and I460V using primers pBFS1-1F / pBFS45-6R. (2) Subsequently, ligate these fragments by overlap extension PCR, and then clone them into pZY900 by the Golden Gate assembly method to obtain plasmid pBFS45. The nucleotide sequence of the coding region of plasmid pBFS45 (i.e., the codon-optimized bFS gene) is shown in SEQ ID NO.1. The primer sequences used for constructing plasmid pBFS45 are shown in Table 12 below:

[0194] Table 12 - Primers required for the construction of plasmid pBFS45

[0195]

[0196] 3. Plasmid pBFS45-P4

[0197] (1) Use the primers pAFS1-P2-1F / R, pAFS1-P2-3F / pBFS45-P4-3R, pBFS45-P4-5F / pBFS45-P4-5R, pBFS45-P4-7F / pAFS1-P2-7R, pAFS1-P2-8F / R shown in Table 13 to amplify Ura3 left arm, tCYC1, pGAL10-pGAL1, tPGK1, and Ura3 right arm from the Saccharomyces cerevisiae strain CEN.PK2-1D respectively.

[0198] (2) The His3 marker was amplified from pRS423 (ATCC87327) using the primers pAFS1-P2-2F / R shown in Table 13.

[0199] (3) Mac-bFS (F11S, M35T, T319S, I434T, I460V) was amplified from the plasmid pBFS45 constructed in the above step using the primers pBFS45-P4-4F / R and pBFS45-P4-6F / R shown in Table 13.

[0200] (4) The plasmid backbone was amplified from pRS426 using the primer pAFS1-P2-9F / R.

[0201] (5) Subsequently, these fragments obtained in the above steps were assembled to obtain the plasmid pBFS45-P4.

[0202] 4. Plasmid pBFS45-P7

[0203] (1) YPRCdelta15 left arm, tCYC1, pGAL10-pGAL1, tPGK1, and YPRCdelta15 right arm were amplified from the Saccharomyces cerevisiae strain CEN.PK2-1D using the primers pAFS1-P5-1F / R, pAFS1-P5-3F / pBFS45-P7-3R, pBFS45-P7-5F / R, pBFS45-P7-7F / pAFS1-P5-7R, and pAFS1-P5-8F / R shown in Table 13, respectively.

[0204] (2) The Trp1 marker was amplified from pRS424 using the primer pAFS1-P5-2F / R shown in Table 13.

[0205] (3) Mac-bFS (F11S, M35T, T319S, I434T, I460V) was amplified from pBFS45 using the primers pBFS45-P7-4F / R and pBFS45-P7-6F / R shown in Table 13.

[0206] (4) The plasmid backbone was amplified from pRS426 using the primer pAFS1-P5-9F / R shown in Table 13, and then these fragments were assembled to obtain the plasmid pBFS45-P7. The primer sequences used for constructing the above plasmids are shown in Table 13 below:

[0207] Table 13 - Primers required for constructing plasmids pBFS45-P4 and pBFS45-P7

[0208]

[0209]

[0210] Example 4: Construction of a High-Yielding β-Farnesene Strain

[0211] 1. Linearize the plasmids pBFS45 and pBFS1 constructed in Example 3 using Mssi, recover the fragments carrying the target genes, transform them into Saccharomyces cerevisiae JCR27, and integrate them into the chromosome according to homologous recombination. The integration site is LEU2, and the screening marker is leucine to construct strains JVA122 and JVA94. After shake-flask fermentation, the yield of strain JVA94 is 203 mg / L, and the yield of strain JVA122 is 437 mg / L. This indicates that the mutation of farnesene synthase plays an important role in improving the yield.

[0212] 2. Linearize the plasmid pBFS45-P4 constructed in Example 3, recover the fragment carrying the target gene, transform it into Saccharomyces cerevisiae JVA122, and integrate it into the chromosome according to homologous recombination. The integration site is URA3, and the screening marker is histidine to construct strain JVA129.

[0213] 3. Linearize pBFS45-P7 constructed in Example 3, recover the fragment carrying the target gene, transform it into Saccharomyces cerevisiae JVA129, and integrate it into the chromosome according to homologous recombination. The integration site is YPRCdelta15, and the screening marker is tryptophan to construct strain JVA139.

[0214] 4. Ferment the strains JVA122, JVA129, and JVA139 in shake flasks respectively. These strains can all exhibit good performance, and the β-farnesene yield can reach a relatively high level, with the yield range being 790 mg / L.

[0215] Example 5: Construction of a Vector for High-Yield Production of Pantothenic Acid While Producing β-Farnesene

[0216] The related genes involved in the embodiments of the present invention include: the native yeast gene acetolactate synthase (ilv2); acetolactate synthase small subunit (ilv6); isomerase (ilv5); dihydroxy-acid dehydratase (ilv3); hydroxymethyltransferase (Ecm31p); ketopantoate reductase (pan5); D-pantothenate synthase (pan6); the gene aspartate 1-decarboxylase (PAND) synthesized from Escherichia coli after codon optimization for Saccharomyces cerevisiae.

[0217] 1. Construction of plasmid pXZ162 (as Figure 4 shown):

[0218] Amplify pSNR52_gRNA.rDNA, KlURA3 from pKlURA3 100 (specific source is shown below) using primers 1621-F / R. Then clone it into pCAS (specific source is shown below) by the method of Golden Gate assembly to obtain plasmid pXZ162. Among them, the sources of pKlURA3 100 and pCAS are: A gRNA-tRNA array for CRISPR-Cas9 based rapid multiplexed genome editing in Saccharomyces cerevisiae;

[0219] Table 14 - Primers required for the construction of plasmid pXZ162

[0220] Primer - pXZ162 Sequence (5’-3’) 1621-F aaaggtctcagatctccaaagtgacaggtgccccgttttagagctagaaatagcaagtt(SEQ ID NO.125) 1621-R aaaggtctcaaaactctagactttttcgatgatgtagtttc(SEQ ID NO.126)

[0221] 2. Construction of plasmid pPT1 (as Figure 1 shown):

[0222] (1) The rDNA left arm was amplified from the yeast S288C genome using primers 1471-F / 1471-R. (2) tCYC1 was amplified from yeast genome 30000B using primers 1472-F / pPT1-2R. (3) Ilv2 was amplified from 30000B using primers pPT1-3F / 3R. (4) pGAL1-pGAL10 was amplified from 30000B using primers pPT1-4F / 4R. (5) Ilv5 was amplified from 30000B using primers pPT1-5F / 5R. (6) tGAL10-pGAL7 was amplified from 30000B using primers pPT1-6F / 6R. (7) Ilv3 was amplified from 30000B using primers pPT1-7F / 7R. (8) tADH1 was amplified from 30000B using primers pPT1-8F / pPT2-8R. (9) pGAL2 was amplified from 30000B using primers pPT2-9F / pPT1-9R. (10) Ilv6 was amplified from 30000B using primers pPT1-10F / pPT1-10R. (11) tERG20 was amplified from 30000B using primers pPT1-11F / pPT2-11R. (12) The rDNA Right arm was amplified from the yeast S288C genome using primers pPT2-12F / 1474-R. (13) The fragments were ligated into one fragment by overlap extension PCR and then plasmid pPT1 was obtained by Gibson assembly with pMD19T (available from TAKARA #D102A).

[0223] Table 15 - Primers required for the construction of plasmid pPT1

[0224]

[0225]

[0226] 3. Construction of plasmid pPT2 (as Figure 2 shown):

[0227] (1) The rDNA left arm was amplified from the yeast S288C genome using primers 1471-F / 1471-R. (2) tCYC1 was amplified from 30000B using primers 1472-F / pPT3-2R. (3) Ecm31P was amplified from 30000B using primers pPT3-3F / 3R. (4) pGAL1-pGAL10 was amplified from 30000B using primers pPT3-4F / 4R. (5) Pan5 was amplified from 30000B using primers pPT3-5F / 5R. (6) tGAL10-pGAL7 was amplified from 30000B using primers pPT3-6F / 6R. (7) Pan6 was amplified from 30000B using primers pPT3-7F / 7R. (8) tADH1 was amplified from 30000B using primers pPT3-8F / pPT2-8R. (9) pGAL2 was amplified from 30000B using primers pPT2-9F / pPT2-9R. (10) PanD was amplified from PanD-Sc using primers pPT2-10F / pPT2-10R. (11) tERG20 was amplified from 30000B using primers pPT2-11F / 11R. (12) The rDNA Right arm was amplified from S288C using primers pPT2-12F / 1474-R. (13) The fragments were ligated into one fragment by overlap extension PCR and then ligated with pMD19T by Gibson assembly to obtain plasmid pPT2.

[0228] Table 16 - Primers required for the construction of plasmid pPT2

[0229]

[0230]

[0231] 4. Construction of plasmid pPT3 (as Figure 3 shown):

[0232] (1) The rDNA left arm was amplified from S288C using primers 1471-F / 1471-R. (2) tCYC1 was amplified from 30000B using primers 1472-F / pPT3-2R. (3) Ecm31P was amplified from 30000B using primers pPT3-3F / 3R. (4) pGAL1-pGAL10 was amplified from 30000B using primers pPT3-4F / 4R. (5) Pan5 was amplified from 30000B using primers pPT3-5F / 5R. (6) tGAL10-pGAL7 was amplified from 30000B using primers pPT3-6F / 6R. (7) Pan6 was amplified from 30000B using primers pPT3-7F / 7R. (8) tADH1 was amplified from 30000B using primers pPT3-8F / 8R. (9) The rDNA Right arm was amplified from S288C using primers pPT3-9F / 1474-R. (10) The fragments were ligated into one fragment by overlap extension PCR and then assembled with pMD19T by Gibson assembly to obtain plasmid pPT3.

[0233] Table 17 - Primers required for the construction of plasmid pPT3

[0234]

[0235]

[0236] Example 6. Construction of strains for simultaneous production of β-farnesene and high-yield pantothenic acid

[0237] 1. The plasmids pPT1 and pPT2 constructed in Example 5 were linearized with Mssi, the fragments carrying the target genes were recovered, and co-transformed with the plasmid pXZ162 constructed in Example 5 into Saccharomyces cerevisiae JVA139. Multisite integration into the chromosome was achieved through CRISPR-Cas9 gene editing, with the integration site being rDNA and the selection marker being uracil, to construct strain JFS1.

[0238] 2. The GAL80 gene of the strain JFS1 was knocked out (construction of the knockout cassette pZY521: Using the genome of yeast 30000B as a template, the left homologous arm of GAL80 was amplified using primers 5201-F / 5211-R; using pZY900 as a template, the selection marker URA3 was amplified using primers 5212-F / 5212-R; the right homologous arm of GAL80 was amplified using primers 5213-F / 5203-R) to eliminate the use of galactose during fermentation, and strain JFS2 was constructed.

[0239] 3. Linearize plasmids pPT1 and pPT3 with Mssi, recover the fragments carrying the target genes, and co-transform them with pXZ162 into Saccharomyces cerevisiae JVA139. Achieve multi-site integration into the chromosome through crispr-cas9 gene editing. The integration site is rDNA, and the selection marker is uracil to construct strain JFS3.

[0240] 4. Knock out the GAL80 gene in strain JFS3 (the same as above) to construct strain JFS4.

[0241] The above primer sequences are shown in Table 18 below:

[0242] Table 18

[0243] Primer Sequence (5’-3’) 5201-F caatggtctaggtagtggcattcg(SEQ ID NO.193) 5211-R cgactcactatagggcgaattgggtacgacgggagtggaaagaacgg(SEQ ID NO.194) 5212-F tcccgttctttccactcccgtcgtacccaattcgccctatagtgag(SEQ ID NO.195) 5212-R gccaagcacagggcaagatgctttcacagcttgtctgtaagcgga(SEQ ID NO.196) 5213-F gcatccgcttacagacaagctgtgaaagcatcttgccctgtgctt(SEQ ID NO.197) 5203-R gagaccaccaagaatacagaagctattat(SEQ ID NO.198)

[0244] Example 7. Fermentation of the strain that simultaneously produces β-farnesene and pantothenic acid in a fermenter

[0245] Refer to the fermentation medium described in the literature (van Hoek, P.; de Hulster, E.; van Dijken, J.P.; Pronk, J.T. Fermentative capacity in high-cell-density fed-batch cultures of baker’s yeast. Biotechnol. Bioeng. 2000, 68, 517 - 523.) and conduct fed-batch fermentation on strain JFS2 constructed in Example 6. Add a covering agent during the fermentation process to achieve in-situ extraction. The covering agent can be dodecane, isopropyl myristate, isopropyl palmitate, liquid paraffin, or white oil. Control the dissolved oxygen above 20% during the fermentation process, with a pH of 5, a glucose concentration of 1 - 2 g / L, and an ethanol concentration below 5 g / L. Finally, in the fermenter, the farnesene yield of the strain is 35 g / L, and the pantothenic acid yield is 1.5 g / L.

[0246] Ferment JFS4 constructed in Example 6 using the same method, with the difference that beta-alanine with a final concentration of 15 g / L is added during the fermentation. Finally, in the fermenter, the farnesene yield of the strain is 45 g / L, and the pantothenic acid yield is 6 g / L.

[0247] Example 8. Construction of a vector for high-yield squalene synthesis

[0248] The relevant genes involved in the embodiments of the present invention include: the native yeast NADH kinase (POS5), alcohol dehydrogenase (ADH2), acetyl-CoA synthetase (ACS2), aldehyde dehydrogenase (ALD6), and squalene synthase (ERG9)

[0249] 1. Plasmid pXZ159 (such asFigure 6 The construction process shown in

[0250] (1) Using the genome of Saccharomyces cerevisiae strain CEN.PK2-1D as a template, fragment 1591 (containing the left homologous arm of the EXG1 locus) was obtained by PCR amplification with primers 1591-F and 1591-R. (2) Fragment 1593 (containing POS5) was obtained by PCR amplification with primers 1593-F and 1593-R. (3) Fragment 1595 (containing the right homologous arm of the EXG1 locus) was obtained by PCR amplification with primers 1595-F and 1595-R. (4) Using pRS426 (ATCC87333) as a template, fragment 1592 (containing the GAL1 promoter) was obtained by PCR amplification with primers 1592-F and 1592-R. (5) Fragment 1594 (containing the CYC1 terminator) was obtained by PCR amplification with primers 1594-F and 1594-R. (6) The fragments were assembled with the pMD19T vector using the Gibson assembly method. After Escherichia coli transformation, digestion verification, and correct sequencing, plasmid pXZ159 was obtained.

[0251] 2. The construction process of plasmid pXZ161 (as shown in Figure 7 )

[0252] pXZ161 is a gRNA plasmid targeting DPP1 and EXG1 sites, and it is a tool plasmid for identifying and cleaving genomic DNA of Saccharomyces cerevisiae at the target site in the Crispr-Cas9 gene editing technology. (1) Using the pstgRNA plasmid (A gRNA-tRNA array for CRISPR-Cas9 based rapid multiplexed genome editing in Saccharomyces cerevisiae) as a template, fragment 161 (containing gRNA at the DPP1 site, gRNA scaffold, tGly, and partial gRNA at the EXG1 site) was obtained by PCR amplification with primers 1611-F and 1611-R; (2) Using the pKlURA3 100 plasmid (the source of the pKlURA3 100: A gRNA-tRNA array for CRISPR-Cas9 based rapid multiplexed genome editing in Saccharomyces cerevisiae;) as a template, fragment 1612 (containing gRNA at the EXG1 site, gRNA scaffold, SUF17 terminator, and URA selection tag) was obtained by PCR amplification with primers 1612-F and 1612-R; (3) The fragment was assembled with the pCas9 vector using the GoldenGate assembly method, and after Escherichia coli transformation, enzyme digestion verification, and correct sequencing, plasmid pXZ161 was obtained.

[0253] 3. Construction process of plasmid pXZ171 (as Figure 5 shown):

[0254] (1) Using the genome of Saccharomyces cerevisiae strain CEN.PK2-1D as a template, fragment 1711 (containing the left homologous arm of the DPP1 locus) was obtained by PCR amplification with primers 1711-F and 1711-R. (2) Fragment 1712 (containing the GAL1 promoter) was obtained by PCR amplification with primers 1712-F and 1712-R. (3) Fragment 1713 (containing ADH2 and the ADH2 terminator) was obtained by PCR amplification with primers 1713-F and 1713-R. (4) Fragment 1714 (containing the GAL10 promoter) was obtained by PCR amplification with primers 1714-F and 1714-R. (5) Fragment 1715 (containing ACS2) was obtained by PCR amplification with primers 1715-F and 1715-R. (6) Fragment 1716 (containing the GAL10 terminator and the GAL7 promoter) was obtained by PCR amplification with primers 1716-F and 1716-R. (7) Fragment 1717 (containing ALD6) was obtained by PCR amplification with primers 1717-F and 1717-R. (8) Fragment 1719 (containing the right homologous arm of the DPP1 locus) was obtained by PCR amplification with primers 1719-F and 1719-R. (9) Using pRS426 (ATCC87333) as a template, fragment 1718 (containing the CYC1 terminator) was obtained by PCR amplification with primers 1718-F and 1718-R. (10) Using fragments 1712, 1713, and 1714 as templates, fragment 17110 was obtained by OE-PCR with primers 1712-F and 1714-R. (11) Using fragments 1715 and 1716 as templates, fragment 17111 was obtained by OE-PCR with primers 1715-F and 1716-R. (12) Using fragments 1717 and 1718 as templates, fragment 17112 was obtained by OE-PCR with primers 1717-F and 1718-R. (13) The fragments 1711, 17110, 17111, 17112, 1719 were assembled with the pMD19T vector using the Gibson assembly method. After Escherichia coli transformation, restriction enzyme digestion verification and correct sequencing, plasmid pXZ171 was obtained.

[0255] 4. Construction process of plasmid pSX01 (as Figure 8 shown):

[0256] (1) Using the genome of the Saccharomyces cerevisiae strain CEN.PK2-1D as a template, the rDNA left arm was amplified from S288C using 1471-F / pSX01-1R. (2) pHXT1 was amplified from the yeast S288C genome using pSX01-2F / R. (3) ERG9 was amplified from S288C using pSX01-3F / R. (4) tERG20 was amplified from 30000B using pSX01-4F / pPT2-11R. (5) The rDNA RIGHT arm was amplified from the yeast S288C genome using pPT2-12F / 1474-R. (6) The above fragments were ligated by overlap extension PCR and then assembled with the pMD19T vector. After correct enzyme digestion verification and sequencing, the plasmid pSX01 was obtained.

[0257] Table 19 - Primers required for vector construction in Example 8

[0258]

[0259]

[0260] Example 9. Construction of a strain for simultaneous production of β-farnesene and high-yield squalene

[0261] 1. The plasmids pXZ171 and pXZ159 were linearized with Mssi, the fragments carrying the target genes were recovered, and co-transformed with pXZ161 into Saccharomyces cerevisiae JVA139. Multisite integration into the chromosome was achieved through crispr-cas9 gene editing at the integration sites DPP1 and exg1, with the screening marker being uracil, to construct the strain JSX1.

[0262] 2. The plasmid pSX01 was linearized with Mssi, the fragments carrying the target genes were recovered, and co-transformed with pXZ162 into Saccharomyces cerevisiae JSX1. Multisite integration into the chromosome was achieved through crispr-cas9 gene editing at the integration site rDNA, with the screening marker being uracil, to construct the strain JSX2.

[0263] 3. The GAL80 gene of the strain JSX2 was knocked out (the same as above) to construct the strain JSX3.

[0264] Example 10. Fermentation of the strain for simultaneous production of β-farnesene and high-yield squalene in a fermenter

[0265] Using the fermentation medium described in the reference (van Hoek, P.; de Hulster, E.; van Dijken, J.P.; Pronk, J.T. Fermentative capacity in high-cell-density fed-batch cultures of baker’s yeast. Biotechnol. Bioeng. 2000, 68, 517-523.), fed-batch fermentation was carried out on the strain JSX3 constructed in Example 9. A covering agent was added during the fermentation process to achieve in-situ extraction. The covering agent can be dodecane, isopropyl myristate, isopropyl palmitate, liquid paraffin, or white oil. The dissolved oxygen was controlled above 20% during the fermentation process, the pH was 5, and the glucose concentration was 1-2 g / L. Finally, in the fermenter, the yield of farnesene by the strain was 37 g / L, and the yield of squalene was 7.5 g / L.

[0266] Example 11. Construction of plasmids required for the simultaneous production of β-farnesene and high-yield fatty acids

[0267] The relevant genes involved in the examples of the present invention include: knocking out the fatty acid degradation-related genes acyl-CoA synthetase FAA1, FAA4, β-oxidase POX1, overexpressing the native acetyl-CoA carboxylase ACC1, fatty acid synthase complex FAS1 and FAS2, and the truncated signal peptide thioesterase tesA derived from Escherichia coli.

[0268] The principle involved in the examples of the present invention: The simultaneous production of farnesene and fatty acids, and the synthesis of both will compete for the substrate acetyl-CoA, resulting in a significant decrease in the yield of farnesene. Therefore, a plasmid was constructed, and the glucose-inducible promoter pHXT1 was used to achieve the synthesis of fatty acids at the early stage of fermentation and the synthesis of farnesene at the later stage of fermentation.

[0269] 1. Construction process of plasmid pZF01 (as Figure 9 shown):

[0270] (1) Using the S288C genome as a template, FAA1 left arm was amplified from S288C with pZF01-1F / 1R. (2) pHXT1 was amplified from S288C with pZF01-2F / 2R. (3) The truncated tesA was amplified from the MG1655 genome with pZF01-3F / 3R. (4) tERG20 was amplified from 30000B with pZF01-4F / 4R. (5) FAA1 RIGHT arm was amplified from S288C with pZF01-5F / 5R. (6) The above fragments were ligated by overlap extension PCR, and then assembled with the pMD19T vector. After correct enzyme digestion verification and sequencing in Escherichia coli transformation, plasmid pZF01 was obtained.

[0271] Table 20 - Primers required for the construction of plasmid pZF01

[0272]

[0273]

[0274] 2. Construction process of plasmid pZF02 (as shown in Figure 10 )

[0275] (1) Using the S288C genome as a template, FAA4 left arm was amplified from S288C with pZF02 - 1F / 1R. (2) pHXT1 was amplified from S288C with pZF02 - 2F / 2R. (3) ACC1 was amplified from S288C with pZF02 - 3F / 3R. (4) tERG20 was amplified from 30000B with pZF02 - 4F / 4R. (4) FAA4 RIGHTarm was amplified from S288C with pZF02 - 5F / 5R. (5) The above fragments were ligated by overlap extension PCR, and then assembled with the pMD19T vector. After Escherichia coli transformation, digestion verification and correct sequencing, plasmid pZF02 was obtained.

[0276] Table 21 - Primers required for the construction of plasmid pZF02

[0277] Primer Sequence (5’-3’) pZF02-1F gatcctctagagattgtttaaacctcggccttcgttcatctc(SEQ ID NO.251) pZF02-1R tattccagatgagacctgcatttgatgcgtacttcttagttttt(SEQ ID NO.252) pZF02-2F agaagtacgcatcaaatgcaggtctcatctggaatat(SEQ ID NO.253) pZF02-2R gaataagctttcttcgctcatgattttacgtatatcaactagttg(SEQ ID NO.254) pZF02-3F gatatacgtaaaatcatgagcgaagaaagcttattcg(SEQ ID NO.255) pZF02-3R cgattagcgttagttttatttcaaagtcttcaacaatttttct(SEQ ID NO.256) pZF02-4F gaagactttgaaataaaactaacgctaatcgataaaacattag(SEQ ID NO.257) pZF02-4R gtaaaaaactatgtcttcctttaaaaaaaatccttggactagtcacg(SEQ ID NO.258) pZF02-5F aggattttttttaaaggaagacatagttttttactttccc(SEQ ID NO.259) pZF02-5R ctgcaggtcgacgatgtttaaacagtcataaattcaaatatcccgtt(SEQ ID NO.260)

[0278] 3. Construction process of plasmid pZF03 (as shown in Figure 11 )

[0279] (1) Gene synthesis of XhoI - pHXT1 - pHXT1 - AvrII; (2) Using the S288C genome as a template, POX1 left arm was amplified from S288C with pZF03 - 1F / 1R. (3) tACT1 was amplified from S288C with pZF03 - 2F / 2R. (4) FAS2 was amplified from S288C with pZF03 - 3F / 3R. (5) FAS1 was amplified from S288C with pZF03 - 4F / 4R. (6) tPGK1 was amplified from 30000B with pZF03 - 5F / 5R. (7) POX1 rightarm was amplified from S288C with pZF03 - 6F / 6R. (8) The vector backbone was amplified from pMD19T with pZF03 - 7F / 7R. (9) The above fragments were ligated by overlap extension PCR, and then digested and ligated with XhoI - pHXT1 - pHXT1 - AvrII obtained by the above gene synthesis. After Escherichia coli transformation, digestion verification and correct sequencing, plasmid pZF03 was obtained.

[0280] Table 22 - Primers required for the construction of plasmid pZF03

[0281] Primer Sequence (5'-3') pZF03-1F gatcctctagagattgcggccgcgtcaaagcactaatacattgggg(SEQ ID NO.261) pZF03-1R tttatccattggaccgtgtaatcgcaatactaatttattatattttcttttt(SEQ ID NO.262) pZF03-2F taaattagtattgcgattacacggtccaatggataaa(SEQ ID NO.263) pZF03-2R ccgtttctactaagaaatagtctctgcttttgtgcgcgt(SEQ ID NO.264) pZF03-3F gcgcacaaaagcagagactatttcttagtagaaacggcgac(SEQ ID NO.265) pZF03-3R aaatcctcgagatgaagccggaagttgagc(SEQ ID NO.266) pZF03-4F cgtaaaatccctaggatggacgcttactccacaag(SEQ ID NO.267) pZF03-4R gatttcaattcaattcaatttaggattgttcatacttttcccag(SEQ ID NO.268) pZF03-5F aacaatcctaaattgaattgaattgaaatcgatagatca(SEQ ID NO.269) pZF03-5R gaaaacaggaaacctctacaacgaacgcagaattttcgag(SEQ ID NO.270) pZF03-6F gaaaattctgcgttcgttgtagaggtttcctgttttcct(SEQ ID NO.271) pZF03-6R gcctgcaggtcgacgatgcggccgctggtaaatgtagtcatgtcattgattc(SEQ ID NO.272) pZF03-7F catttaccagcggccgcatcgtcgacctgcaggca(SEQ ID NO.273) pZF03-7R gtgctttgacgcggccgcaatctctagaggatccccgg(SEQ ID NO.274)

[0282] 4. Construction process of plasmid pZF04 (as shown in Figure 12 ):

[0283] pZF04 is a gRNA plasmid targeting FAA1, FAA4, and POX1 sites, and is a tool plasmid for identifying and cleaving the genomic DNA of Saccharomyces cerevisiae at the target site in the Crispr-Cas9 gene editing technology. (1) Using the pstgRNA plasmid (A gRNA-tRNA array for CRISPR-Cas9 based rapid multiplexed genome editing in Saccharomyces cerevisiae) as a template, the gRNA containing the POX1 site, gRNA scaffold, tGly, and partial gRNA of the FAA1 site was obtained by PCR amplification with primers pZF04-1F and pZF04-1R; (2) Using the pstgRNA plasmid as a template, the gRNA containing partial FAA1 site, gRNA scaffold, tGly, and partial FAA4 site gRNA was obtained by PCR amplification with primers pZF04-2F and pZF04-2R; (3) Using the pKlURA3 100 plasmid (source of the pKlURA3 100: A gRNA-tRNA array for CRISPR-Cas9 based rapid multiplexed genome editing in Saccharomyces cerevisiae;) as a template, the fragment containing the FAA4 site gRNA, gRNA scaffold, SUF17 terminator, and URA selection tag was obtained by PCR amplification with primers pZF04-3F and pZF04-3R; (4) The fragment was assembled with the pCas9 vector using the Golden Gate assembly method, followed by Escherichia coli transformation, restriction enzyme digestion verification, and sequencing. After correct sequencing, plasmid pZF04 was obtained.

[0284] Table 23 - Primers required for the construction process of plasmid pZF04

[0285] Primer Sequence (5'-3') pZF04-1F aaaggtctcagatccacatttttagagtcatcccgttttagagctagaaatagcaagtt(SEQ ID NO.275) pZF04-1R aaaggtctcacctgtcctcagatgcgcaagcccggaatcgaaccggg(SEQ ID NO.276) pZF04-2F aaaggtctcacaggaggcaaaggttttagagctagaaatagcaagtta(SEQ ID NO.277) pZF04-2R aaaggtctcatcttcaggattctgcgcaagcccggaatcgaaccggg(SEQ ID NO.278) pZF04-3F aaaggtctcaaagaaactgctggttttagagctagaaatagcaagtta(SEQ ID NO.279) pZF04-3R aaaggtctcaaaactctagactttttcgatgatgtagtttc(SEQ ID NO.280)

[0286] Example 12. Construction of strains for simultaneous production of β-farnesene and high-yield fatty acids

[0287] 1. Linearize plasmid pZF01 and pZF02 with Mssi, linearize plasmid pZF03 with NotI, recover the fragments with the target genes, and co-transform them with pZF04 into Saccharomyces cerevisiae JVA139. Through CRISPR-Cas9 gene editing, multi-site integration into the chromosome is achieved at the integration sites of POX1, FAA1, and FAA4, with the selection marker being uracil, to construct strain JZF01.

[0288] 2. Knock out the GAL80 gene (knockout cassette pZY521) of the said strain JZF01 to eliminate the use of galactose during fermentation, and construct and obtain strain JZF02.

[0289] Example 13. Fermentation of the strain for spatio-temporal synthesis of farnesene and fatty acids in a fermenter

[0290] Refer to the fermentation medium described in the literature (van Hoek, P.;de Hulster, E.;van Dijken, J.P.;Pronk, J.T. Fermentative capacity in high-cell-density fed-batch cultures of baker’s yeast. Biotechnol. Bioeng. 2000, 68, 517 - 523.), and perform fed-batch fermentation on the strain JZF02 constructed in Example 12. Add a covering agent during fermentation to achieve in-situ extraction. The covering agent can be dodecane, isopropyl myristate, isopropyl palmitate, liquid paraffin, or white oil. Control the pH to 5 during fermentation. Regulate the glucose concentration so that fatty acids are accumulated in the early stage of fermentation and farnesene is accumulated in the later stage of fermentation. Finally, the farnesene yield of the strain is 43 g / L, and the free fatty acid yield is 6.8 g / L.

[0291] Example 14. Construction of plasmids required for the simultaneous production of β-farnesene and vitamin B2

[0292] 1. Construction process of plasmid pVB1 (as Figure 13 shown):

[0293] (1) The rDNA left arm was amplified from S288C using primers 1471-F / 1471-R. (2) tCYC1 was amplified from 30000B using primers 1472-F / pVB1-2R. RIB1 was amplified from 30000B using primers pVB1-3F / 3R. (3) pGAL1-pGAL10 was amplified from 30000B using primers pVB1-4F / 4R. (4) RIB7 was amplified from 30000B using primers pVB1-5F / 5R. (5) tGAL10-pGAL7 was amplified from 30000B using primers pVB1-6F / 6R. (6) RIB2 was amplified from 30000B using primers pVB1-7F / 7R. (7) tADH1 was amplified from 30000B using primers pVB1-8F / 8R. (8) The rDNA Right arm was amplified from S288C using primers pVB1-9F / 1474-R. (9) The fragments were ligated into one fragment by overlap extension PCR and then assembled with pMD19T by Gibson assembly to obtain plasmid pVB1.

[0294] Table 24 - Primers required for the construction of plasmid pVB1

[0295]

[0296] 2. Construction process of plasmid pVB2 (as Figure 14 shown):

[0297] (1) The rDNA left arm was amplified from S288C using primers 1471-F / 1471-R. (2) tCYC1 was amplified from 30000B using primers 1472-F / pVB2-2R. (3) RIB3 was amplified from 30000B using primers pVB2-3F / 3R. (4) pGAL1-pGAL10 was amplified from 30000B using primers pVB2-4F / 4R. (5) RIB4 was amplified from 30000B using primers pVB2-5F / 5R. (6) tGAL10-pGAL7 was amplified from 30000B using primers pVB2-6F / 6R. (7) RIB5 was amplified from 30000B using primers pVB2-7F / 7R. (8) tADH1 was amplified from 30000B using primers pVB2-8F / 8R. (9) The rDNA Right arm was amplified from S288C using primers pVB2-9F / 1474-R. (10) The fragments were ligated into one fragment by overlap extension PCR and then assembled with pMD19T by Gibson assembly to obtain plasmid pVB2.

[0298] Table 25 - Primers required for the construction of plasmid pVB2

[0299]

[0300] Example 15: Construction of Strains Required for Simultaneous Production of β-Farnesene and Vitamin B2

[0301] 1. Linearize plasmids pVB1 and pVB2 with Mssi, recover the fragments carrying the target genes, and co-transform them with pXZ162 into Saccharomyces cerevisiae JVA139. Achieve multi-site integration into the chromosome through crispr-cas9 gene editing. The integration site is rDNA, and the selection marker is uracil to construct strain JVB01.

[0302] 2. Knock out the GAL80 gene (knockout cassette pZY521) of the strain JVB01 to eliminate the use of galactose during fermentation, and construct strain JVB02.

[0303] Example 16: Fermentation Tank Fermentation of Strains for Farnesene and Vitamin B2 Synthesis

[0304] Refer to the fermentation medium described in the literature (van Hoek, P.; de Hulster, E.; van Dijken, J.P.; Pronk, J.T. Fermentative capacity in high-cell-density fed-batch cultures of baker’s yeast. Biotechnol. Bioeng. 2000, 68, 517-523.), and perform fed-batch fermentation on the strain JVB02 constructed in Example 15. Add a covering agent during the fermentation process to achieve in-situ extraction. The covering agent can be dodecane, isopropyl myristate, isopropyl palmitate, liquid paraffin, or white oil. Control the pH at 5 during the fermentation process. The strain can produce high-yield vitamin B2 while producing farnesene. Finally, the farnesene yield of the strain is 37 g / L, and the vitamin B2 yield is 2.5 g / L.

[0305] The above embodiments are preferred embodiments of the present invention, but the embodiments of the present invention are not limited by the above embodiments. Any other changes, modifications, substitutions, combinations, and simplifications made without departing from the spirit and principle of the present invention shall be equivalent replacement methods and are all included in the protection scope of the present invention.

[0306] Finally, it should also be noted that the term "comprising", "including" or any other variant thereof is intended to cover non-exclusive inclusion, so that a process, method, article or device including a series of elements not only includes those elements, but also includes other elements not explicitly listed, or further includes elements inherent to such process, method, article or device.

[0307] Although the preferred embodiments of the present invention have been described, those skilled in the art can make additional changes and modifications to these embodiments once they know the basic creative concept. Therefore, the appended claims are intended to be construed to include the preferred embodiments as well as all changes and modifications that fall within the scope of the present invention.

[0308] Obviously, those skilled in the art can make various changes and modifications to the present invention without departing from the spirit and scope of the present invention. Thus, if these modifications and variations of the present invention fall within the scope of the claims of the present invention and their equivalent technologies, the present invention is also intended to include these modifications and variations. Sequence Listing <110> Wuhan University <120> A recombinant microorganism for simultaneously producing sesquiterpenes and primary metabolites and its application <160> 317 <170> SIPOSequenceListing 1.0 <210> 1 <211> 1725 <212> DNA <213> Artificial Sequence <400> 1 atgtctacta tccctgtttc cagtgtttct tcctctagtt ctgcctctcc attggtattg 60 gatgataaat tatctaccaa gcaagatgtt gtcagacaca ccaccaactt ttctgcttct 120 atctggggtg accaattcct aacataccac gaaccagaag atttggtcat caagaagcaa 180 caagttgaac aattgaagga agaagttaag aaggaattga tggctatcaa gggttctaac 240 gacccacaac aacacatcaa attaatggaa ttgatcgact ctgttcaaag attgggtatc 300 gcttaccatt tcgaagaaga aattgaagaa gctttgcaac acattcacgt tacttatggt 360 gaacactggg ttgacaagga aaacttgcaa tccgtcagct tgtggttcag attactaaga 420 gaacactggg ttgacaagga aaacttgcaa tccgtcagct tgtggttcag attactaaga 420 caacaaggtt tcaacgtttc ttccggtgtt ttcaaggact acatggacga aaaaggtaac 480 caacaaggtt tcaacgtttc ttccggtgtt ttcaaggact acatggacga aaaaggtaac 480 ttcaaggaat ctttgtgtaa cgatgctcaa ggtatcttgg ctttgtacga agctgcttac 540 ttcaaggaat ctttgtgtaa cgatgctcaa ggtatcttgg ctttgtacga agctgcttac 540 atgagagttg aaggtgaaac cagattggac aaggctttgg aattcaccaa ggttcatttg 600 atgagagttg aaggtgaaac cagattggac aaggctttgg aattcaccaa ggttcatttg 600 gatatcattt ctaaggatcc atcttgtgat tcttacttga gaactcgtgt tcaccaagcc 660 gatatcattt ctaaggatcc atcttgtgat tcttacttga gaactcgtgt tcaccaagcc 660 ctgaaacaac cattaagaag aagattggcc agaatcgaag cattacacta catgccagtc 720 ctgaaacaac cattaagaag aagattggcc agaatcgaag cattacacta catgccagtc 720 taccaacaag actcttctca caacgaagtt ttactaaagt tggctaaggt cgatttctct 780 taccaacaag actcttctca caacgaagtt ttactaaagt tggctaaggt cgatttctct 780 gttttgcaat ctatgcacaa gaaagaattg tcacatatct gtaagtggtg gaaggacttg 840 gttttgcaat ctatgcacaa gaaagaattg tcacatatct gtaagtggtg gaaggacttg 840 gatttgcaaa acaagttgcc atacgttcgt gacagagtcg ttgaaggtta cttctggatt 900 gatttgcaaa acaagttgcc atacgttcgt gacagagtcg ttgaaggtta cttctggatt 900 ttgtccatct actacgaacc tcaacatgcc agaaccagaa tgttcttgat gaagtcttgt 960 ttgtccatct actacgaacc tcaacatgcc agaaccagaa tgttcttgat gaagtcttgt 960 atgtggcttg ttgttttgga tgacactttt gacaattacg gtacctacga agagttggaa 1020 atgtggcttg ttgttttgga tgacactttt gacaattacg gtacctacga agagttggaa 1020 attttcgctc aagctgtcga aagatggagt atttcatgca tggatatgtt gccagaatat 1080 attttcgctc aagctgtcga aagatggagt atttcatgca tggatatgtt gccagaatat 1080 atgaaattga tttaccaaga attagttaac ttgcacgtcg acatggaaga atccttggaa 1140 aaggaaggta agacttacca aatccattac gtcaaggaaa tggccaagga attggtccgt 1200 aactacttgg tcgaagcccg ttggttgaag gagggttaca tgccaacctt agaagaatac 1260 atgtccgtat ccatggttac cggtacttac ggtttgatga ctgctagatc ctacgttggt 1320 agagacgaca ttgtcactga agacactttc aaatgggttt cttcttaccc accaatcgtc 1380 aaggcttcct gtgttattgt ccgtctgatg gacgatattg tctcccacaa ggaagaacaa 1440 gaaagaggtc acgttgcttc ttccattgaa tgttacagca aggaatctgg tgctaccgaa 1500 gaagaagctt gtgaatacat ctcctccaag gtcgaagacg cttggaaggt catcaacaga 1560 gaatcattaa gaccaactgc tgttccattc ccattgttga tgccagctat taatttggcc 1620 agaatgtgtg aagtcttata ctctgtcaac gatggtttca ctcacgctga aggcgacatg 1680 aagtcctata tgaagtccta ctttgtccac ccaatggtca tttga 1725 <210> 2 <211> 381 <212> DNA <213> Artificial Sequence <400> 2 atgattagaa ccatgttgca aggaaagtta catagggtaa aggttactca cgccgatttg 60 cattatgagg gttcctgtgc tattgatcag gatttcctag acgctgcagg cattttagaa 120 aacgaagcga ttgacatctg gaatgttacc aatggtaaga gatttagtac atatgccatc 180 gctgcagaaa gaggcagcag aattatctca gtgaatggag ctgccgcgca ttgtgcttct 240 gttggcgata ttgtaattat agcatcattc gttactatgc cagacgaaga agctagaaca 300 tggagaccga atgtcgctta ctttgaaggt gataatgaaa tgaagagaac tgctaaagct 360 ataccagtcc aggttgcttg a 381 <210> 3 <211> 12416 <212> DNA <213> Artificial Sequence <400> 3 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 60 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 120 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 180 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc 240 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat 300 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt 360 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt acccggggat 420 cctctagaga ttgtttaaac tttcctctaa tcaggttcca ccaaacagat accccggtgt 480 ttcacggaat ggtacgtttg atatcgctga tttgagagga ggttacactt gaagaatcac 540 agtcttgcga ccggctattc aacaaggcat tcccccaagt ttgaattctt tgaaatagat 600 tgctattagc tagtaatcca ccaaatcctt cgctgctcac caatggaatc gcaagatgcc 660 cacgatgaga ctgttcaggt taaacgcaaa agaaacacac tctgggaatt tcttcccaaa 720 ttgtatctct caatacgcat caacccatgt caattaaaca cgctgtatag agactaggca 780 gatctgacga tcacctagcg actctctcca ccgtttgacg aggccattta caaaaacata 840 acgaacgaca agcctactcg aattcgtttc caaactcttt tcgaacttgt cttcaactgc 900 tttcgcatga agtacctccc aactactttt cctcacactt gtactccatg actaaacccc 960 ccctcccatt acaaactaaa atcttacttt tattttcttt tgccctctct gtcgctctgc 1020 cttaactacg tatttctcgc cgagaaaaac ttcaatttaa gctattctcc aaaaatctta 1080 gcgtatattt tttttccaaa gtgagcaaat taaagccttc gagcgtccca aaaccttctc 1140 aagcaaggtt ttcagtataa tgttacatgc gtacacgcgt ttgtacagaa aaaaaagaaa 1200 aatttgaaat ataaataacg ttcttaatac taacataact ataaaaaaat aaatagggac 1260 ctagacttca ggttgtctaa ctccttcctt ttcggttaga gcggatgtgg ggggagggcg 1320 tgaatgtaag cgtgacataa ctaattacat gatatcgaca aaggaaaagg ggcctgttca 1380 gtgcttaccg cctgtacgct tatgacgtaa ttcagtctgt tgtctttcaa cttctgggtc 1440 aaaatttatg aactcgtcta gaccgctacc acctgccacc attggcaaaa caggaacttt 1500 tttatcaact tccacttcaa gcaaaactgg gcccttggta gaaacgaatt ctttcaactt 1560 agcgtccaat tcctcttgct tcttgactct taaacctttt aaacccatag cctccgctag 1620 ttttatgaaa tcagggttca attgatgtgt gtgggaataa cgatgttcgt agaacaggga 1680 ttgccattga gtaaccatac cttgctcttc attgttcaaa atcaaaatct tcactggagt 1740 accagcttga acggcagaac tcaattccgt tagagtcatg ttaaaggatg cgtcaccatc 1800 aatgtcaata accaaagatt ctggctttgc aacttgagca ccgatggcgg cagggagacc 1860 gtaacccatc gtacctaaac cacctgatgt gatgaaagta tgtggatttc tccatgtcca 1920 gtgttgagca gcccacattt gatgttgccc cacacccgtt gtaacaatga catgtcttcc 1980 tgtgtcgttg gcaaccttgg atagtttctt tataaccgtc tgtggtttaa ttttagatcc 2040 tggagtctcc tccatataag cgtatgggta ttccttcttc catttattta tttgagcaaa 2100 ccattcagac ctctccttaa ctgggaaaat ctttgacatc attttgccca gattggtcgt 2160 agcatcacct tccactgcta tttgagtttg aacaaccttg tttatgtttt ttggactaac 2220 ctcgaaatga ataataccac ctctaccctc ggcagctgca cgacgagctt ctggagcgaa 2280 tttagaaata ttaccagtga cacggtcgtc gaatctagca ccaactgcaa ttatcaagtc 2340 ggcattttgc actgccaggt tggcagtagc acaaccgtgc ataccaagca tatccaatga 2400 ttttggatct tcttggtcga atgaacctaa accttgtaaa gtagtggtga caggtatttg 2460 agcacggtca cttaattctt ttagtaatct tggaccatct gcatggttta aaataccagc 2520 accgacgtat aagacaggtt tctttgccaa gttgatcaaa tctgctgctt tattgatact 2580 ttgcatgaca aattcatctt gtgcgcgact ggttaattgg tttagtgcgt ttgatggaag 2640 agttgttttt gttggaattg gatttcttaa gatagctgct gtaacatcct ttggtaaatc 2700 gaccaagact ggtcccggtc taccgctcgt ggcaatttca aaagcctcgt taatacgcaa 2760 tggcaattct tccacggact tgaccatgac attccatttc gtacaagatc tagaaatacc 2820 aacgacgtca gcctcttgga aagcatcagt accgatagca ctagttggga cttgccctgt 2880 aaagacaacc attggaatcc cgtctgcaaa ggcatctgcc attggagtaa cgacattggt 2940 ggcacctggc ccagaagtaa ccaagacaac acctggttta ccagaagctc tggcgtagcc 3000 ttctgccatg tgaccggcac cttgttcgtg ttttggaaga acgaagttga atttatcact 3060 gttatgaatg gcatcgtaaa caggtaggat agcaccacct ggataaccaa atacagtatc 3120 aacgttttgt ctggacatca tttcgttaaa tatttgacca ccagttaaac cgacgaaaga 3180 ggtatccatg tcaggctcag cgcgtagttt cttagccaat tttgaaggtt cagcgggctg 3240 ttctaatgga tcaacattga aacttggagc aggctctggc cttttagagg ctggtaatgg 3300 agacgcactg taataacggg aagacgaact ataaaagcgc tgcgcgagag ctactgatct 3360 catggcaggt gtgttgcggt atgctatatg ttgaaagcaa cgcttaatag cgaagttttt 3420 tagcgtagat tgtctgatca ttatagtttt ttctccttga cgttaaagta tagaggtata 3480 ttaacaattt tttgttgata cttttatgac atttgaataa gaagtaatac aaaccgaaaa 3540 tgttgaaagt attagttaaa gtggttatgc agcttttgca tttatatatc tgttaataga 3600 tcaaaaatca tcgcttcgct gattaattac cccagaaata aggctaaaaa actaatcgca 3660 ttattatcct atggttgtta atttgattcg ttgatttgaa ggtttgtggg gccaggttac 3720 tgccaatttt tcctcttcat aaccataaaa gctagtattg tagaatcttt attgttcgga 3780 gcagtgcggc gcgaggcaca tctgcgtttc aggaacgcga ccggtgaaga ccaggacgca 3840 cggaggagag tcttccgtcg gagggctgtc gcccgctcgg cggcttctaa tccgtacttc 3900 aatatagcaa tgagcagtta agcgtattac tgaaagttcc aaagagaagg tttttttagg 3960 ctaagataat ggggctcttt acatttccac aacatataag taagattaga tatggatatg 4020 tatatggtgg tattgccatg taatatgatt attaaacttc tttgcgtcca tccaaaaaaa 4080 aagtaagaat ttttgaaaat tcaatataaa tgttgagaac tcaagccgcc agattgatct 4140 gcaactcccg tgtcatcact gctaagagaa cctttgcttt ggccacccgt gctgctgctt 4200 acagcagacc agctgcccgt ttcgttaagc caatgatcac tacccgtggt ttgaagcaaa 4260 tcaacttcgg tggtactgtt gaaaccgtct acgaaagagc tgactggcca agagaaaagt 4320 tgttggacta cttcaagaac gacacttttg ctttgatcgg ttacggttcc caaggttacg 4380 gtcaaggttt gaacttgaga gacaacggtt tgaacgttat cattggtgtc cgtaaagatg 4440 gtgcttcttg gaaggctgcc atcgaagacg gttgggttcc aggcaagaac ttgttcactg 4500 ttgaagatgc tatcaagaga ggtagttacg ttatgaactt gttgtccgat gccgctcaat 4560 cagaaacctg gcctgctatc aagccattgt tgaccaaggg taagactttg tacttctccc 4620 acggtttctc cccagtcttc aaggacttga ctcacgttga accaccaaag gacttagatg 4680 ttatcttggt tgctccaaag ggttccggta gaactgtcag atctttgttc aaggaaggtc 4740 gtggtattaa ctcttcttac gccgtctgga acgatgtcac cggtaaggct cacgaaaagg 4800 cccaagcttt ggccgttgcc attggttccg gttacgttta ccaaaccact ttcgaaagag 4860 aagtcaactc tgacttgtac ggtgaaagag gttgtttaat gggtggtatc cacggtatgt 4920 tcttggctca atacgacgtc ttgagagaaa acggtcactc cccatctgaa gctttcaacg 4980 aaaccgtcga agaagctacc caatctctat acccattgat cggtaagtac ggtatggatt 5040 acatgtacga tgcttgttcc accaccgcca gaagaggtgc tttggactgg tacccaatct 5100 tcaagaatgc tttgaagcct gttttccaag acttgtacga atctaccaag aacggtaccg 5160 aaaccaagag atctttggaa ttcaactctc aacctgacta cagagaaaag ctagaaaagg 5220 aattagacac catcagaaac atggaaatct ggaaggttgg taaggaagtc agaaagttga 5280 gaccagaaaa ccaataattt gccagcttac tatccttctt gaaaatatgc actctatatc 5340 ttttagttct taattgcaac acatagattt gctgtataac gaattttatg ctatttttta 5400 aatttggagt tcagtgataa aagtgtcaca gcgaatttcc tcacatgtag ggaccgaatt 5460 gtttacaagt tctctgtacc accatggaga catcaaagat tgaaaatcta tggaaagata 5520 tggacggtag caacaagaat atagcacgag ccgcgaagtt catttcgtta cttttgatat 5580 cgctcacaac tattgcgaag cgcttcagtg aaaaaatcat aaggaaaagt tgtaaatatt 5640 attggtagta ttcgtttggt aaagtagagg gggtaatttt tcccctttat tttgttcata 5700 cattcttaaa ttgctttgcc tctccttttg gaaagctata cttcggagca ctgttgagcg 5760 aaggctcatt agatatattt tctgtcattt tccttaaccc aaaaataagg gaaagggtcc 5820 aaaaagcgct cggacaactg ttgaccgtga tccgaaggac tggctataca gtgttcacaa 5880 aatagccaag ctgaaaataa tgtgtagcta tgttcagtta gtttggctag caaagatata 5940 aaagcaggtc ggaaatattt atgggcatta ttatgcagag catcaacatg ataaaaaaaa 6000 acagttgaat attccctcaa aaatgggctt gttaacgaaa gttgctacat ctagacaatt 6060 ctctacaacg agatgcgttg caaagaagct caacaagtac tcgtatatca tcactgaacc 6120 taagggccaa ggtgcgtccc aggccatgct ttatgccacc ggtttcaaga aggaagattt 6180 caagaagcct caagtcgggg ttggttcctg ttggtggtcc ggtaacccat gtaacatgca 6240 tctattggac ttgaataaca gatgttctca atccattgaa aaagcgggtt tgaaagctat 6300 gcagttcaac accatcggtg tttcagacgg tatctctatg ggtactaaag gtatgagata 6360 ctcgttacaa agtagagaaa tcattgcaga ctcctttgaa accatcatga tggcacaaca 6420 ctacgatgct aacatcgcca tcccatcatg tgacaaaaac atgcccggtg tcatgatggc 6480 catgggtaga cataacagac cttccatcat ggtatatggt ggtactatct tgcccggtca 6540 tccaacatgt ggttcttcga agatctctaa aaacatcgat atcgtctctg cgttccaatc 6600 ctacggtgaa tatatttcca agcaattcac tgaagaagaa agagaagatg ttgtggaaca 6660 tgcatgccca ggtcctggtt cttgtggtgg tatgtatact gccaacacaa tggcttctgc 6720 cgctgaagtg ctaggtttga ccattccaaa ctcctcttcc ttcccagccg tttccaagga 6780 gaagttagct gagtgtgaca acattggtga atacatcaag aagacaatgg aattgggtat 6840 tttacctcgt gatatcctca caaaagaggc ttttgaaaac gccattactt atgtcgttgc 6900 aaccggtggg tccactaatg ctgttttgca tttggtggct gttgctcact ctgcgggtgt 6960 caagttgtca ccagatgatt tccaaagaat cagtgatact acaccattga tcggtgactt 7020 caaaccttct ggtaaatacg tcatggccga tttgattaac gttggtggta cccaatctgt 7080 gattaagtat ctatatgaaa acaacatgtt gcacggtaac acaatgactg ttaccggtga 7140 cactttggca gaacgtgcaa agaaagcacc aagcctacct gaaggacaag agattattaa 7200 gccactctcc cacccaatca aggccaacgg tcacttgcaa attctgtacg gttcattggc 7260 accaggtgga gctgtgggta aaattaccgg taaggaaggt acttacttca agggtagagc 7320 acgtgtgttc gaagaggaag gtgcctttat tgaagccttg gaaagaggtg aaatcaagaa 7380 gggtgaaaaa accgttgttg ttatcagata tgaaggtcca agaggtgcac caggtatgcc 7440 tgaaatgcta aagccttcct ctgctctgat gggttacggt ttgggtaaag atgttgcatt 7500 gttgactgat ggtagattct ctggtggttc tcacgggttc ttaatcggcc acattgttcc 7560 cgaagccgct gaaggtggtc ctatcgggtt ggtcagagac ggcgatgaga ttatcattga 7620 tgctgataat aacaagattg acctattagt ctctgataag gaaatggctc aacgtaaaca 7680 aagttgggtt gcacctccac ctcgttacac aagaggtact ctatccaagt atgctaagtt 7740 ggtttccaac gcttccaacg gttgtgtttt agatgcttga gcgaatttct tatgatttat 7800 gatttttatt attaaataag ttataaaaaa aataagtgta tacaaatttt aaagtgactc 7860 ttaggtttta aaacgaaaat tcttattctt gagtaactct ttcctgtagg tcaggttgct 7920 ttctcaggta tagcatgagg tcgctcttat tgaccacacc tctaccgggg ttgcaatttc 7980 tttttctatt agtagctaaa aatgggtcac gtgatctata ttcgaaaggg gcggttgcct 8040 caggaaggca ccggcggtct ttcgtccgtg cggagatatc tgcgccgttc aggggtccat 8100 gtgccttgga cgatattaag gcagaaggca gtatcggggc ggatcactcc gaaccgagat 8160 tagttaagcc cttcccatct caagatgggg agcaaatggc attatactcc tgctagaaag 8220 ttaactgtgc acatattctt aaattataca acattctgga gagctattgt tcaaaaaaca 8280 aacatttcgc aggctaaaat gtggagatag gataagtttt gtagacatat ataaacaatc 8340 agtaattgga ttgaaaattt ggtgttgtga attgctcttc attatgcacc ttattcaatt 8400 atcatcaaga atagtaatag ttaagtaaac acaagattaa cataataaaa aaaataattc 8460 tttcataatg ctgagatcgt tattgcaaag cggccaccgc agggtggttg cttcttcatg 8520 tgctaccatg gtgcgttgca gttcctcgtc gacctccgcg ttggcgtaca agcagatgca 8580 cagacacgca acaagacctc ccttgcccac actagacact ccttcctgga atgccaacag 8640 tgccgtttca tccatcattt acgaaacacc agcgccttct cgtcaaccaa gaaaacagca 8700 tgtcttgaac tgtttggtgc aaaacgaacc cggtgtcttg tccagagtct cgggtacgtt 8760 agctgccaga ggctttaaca tcgattcgtt ggtcgtgtgc aacaccgagg tcaaagacct 8820 aagtagaatg accattgttt tgcaagggca agatggcgta gtcgaacaag cacgcagaca 8880 aatcgaagac ttggtccccg tctacgccgt cctagactat accaattctg agatcatcaa 8940 aagagagcta gtgatggcca gaatctctct attgggtact gaatacttcg aagacctact 9000 attgcaccac cacacttcca ccaatgctgg cgccgctgac tcccaagaat tggtcgccga 9060 aatcagagaa aagcaattcc accctgccaa cttgcccgcc agtgaggtat taaggttgaa 9120 gcacgagcat ttgaacgata tcaccaactt gaccaacaac tttggaggtc gtgtcgtcga 9180 catcagcgaa acaagctgta ttgtggagtt gtctgcaaaa cccacacgta tctctgcctt 9240 cttgaagttg gtcgagccat tcggtgtcct agagtgtgca agaagcggta tgatggcatt 9300 gccaagaact cctttgaaga caagcaccga ggaagctgcc gacgaagacg aaaagatcag 9360 cgaaatcgtc gacatttccc aactaccacc tggttagaac taacgctaat cgataaaaca 9420 ttagatttca aactagataa ggaccatgta taagaactat atacttccaa tataatatag 9480 tataagcttt aagatagtat ctctcgatct accgttccac gtgactagtc caaggatttt 9540 ttttaacagg tgccccgggt aacccagttc ctcactattt tttactgcgg aagcggaagc 9600 ggaaaatacg gaaacgcgcg ggaacataca aaacatacaa aatatacctt tctcacacaa 9660 gaaatatatg ctacttgcaa aatatcatac caaaaaactt ttcacaaccg aaaccaaaac 9720 caacggatat catacattac actaccacca ttcaaacttt actactatcc tcccttcagt 9780 ttcccttttt ctgccttttt cggtgacgga aatacgcttc agagacccta aagggaaatc 9840 catgccataa caggaaagta acatcccaat gcggactata ccaccccacc acactcctac 9900 caataacggt aactattcta tgttttctta ctcctatgtc tattcatctt tcatctgact 9960 acctaatact atgcaaaaat gtaaaatcat cacacaaaac ataaacaatc aaaatcagcc 10020 atttccgcac cttttcctct gtccactttc aaccgtccct ccaaatgtaa aatggcctat 10080 cggaatacat tttctacatc ctaactacta taaaacaacc tttagactta cgtttgctac 10140 tctcatggtt taaacatcgt cgacctgcag gcatgcaagc ttggcgtaat catggtcata 10200 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag 10260 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg 10320 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca 10380 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc 10440 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg 10500 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa 10560 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga 10620 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag 10680 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct 10740 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg 10800 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc 10860 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt 10920 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta 10980 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac 11040 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac 11040 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc 11100 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc 11100 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat 11160 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat 11160 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc 11220 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc 11220 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt 11280 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt 11280 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta 11340 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta 11340 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct 11400 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct 11400 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg 11460 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg 11460 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga 11520 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga 11520 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt 11580 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt 11580 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt 11640 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt 11640 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt 11700 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt 11700 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat 11760 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat 11760 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc 11820 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc 11820 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc 11880 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc 11880 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat 11940 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat 11940 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag 12000 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag 12000 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt 12060 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt 12060 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc 12120 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc 12120 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa 12180 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa 12180 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg 12240 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg 12240 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa 12300 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa 12300 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac 12360 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac 12360 cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtc 12416 <210> 4 <211> 9866 <212> DNA <213> Artificial Sequence <400> 4 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 60 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 120 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 180 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc 240 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat 300 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt 360 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt acccggggat 420 cctctagaga ttgtttaaac tttcctctaa tcaggttcca ccaaacagat accccggtgt 480 ttcacggaat ggtacgtttg atatcgctga tttgagagga ggttacactt gaagaatcac 540 agtcttgcga ccggctattc aacaaggcat tcccccaagt ttgaattctt tgaaatagat 600 tgctattagc tagtaatcca ccaaatcctt cgctgctcac caatggaatc gcaagatgcc 660 cacgatgaga ctgttcaggt taaacgcaaa agaaacacac tctgggaatt tcttcccaaa 720 ttgtatctct caatacgcat caacccatgt caattaaaca cgctgtatag agactaggca 780 gatctgacga tcacctagcg actctctcca ccgtttgacg aggccattta caaaaacata 840 acgaacgaca agcctactcg aattcgtttc caaactcttt tcgaacttgt cttcaactgc 900 tttcgcatga agtacctccc aactactttt cctcacactt gtactccatg actaaacccc 960 ccctcccatt acaaactaaa atcttacttt tattttcttt tgccctctct gtcgctctgc 1020 cttaactacg tatttctcgc cgagaaaaac ttcaatttaa gctattctcc aaaaatctta 1080 gcgtatattt tttttccaaa gtgagcaaat taaagccttc gagcgtccca aaaccttctc 1140 aagcaaggtt ttcagtataa tgttacatgc gtacacgcgt ttgtacagaa aaaaaagaaa 1200 aatttgaaat ataaataacg ttcttaatac taacataact ataaaaaaat aaatagggac 1260 ctagacttca ggttgtctaa ctccttcctt ttcggttaga gcggatgtgg ggggagggcg 1320 tgaatgtaag cgtgacataa ctaattacat gatatcgaca aaggaaaagg ggcctgttta 1380 cttttcgtta attgaagaga ggaattcatt ccataagtcc tccttaactt tgaaagtatg 1440 agtacctctc tcaggaaatg tcctatcctc tacacttgca atgtactcct tcaagccctg 1500 agtggcaata tctgtcatat ttacagcttg cttcacaaat tttgggacag aatcgccttg 1560 catcccgaga aggtcagata taactagaac ttgtccactg gtaccgttac ctgcaccgat 1620 acctattgtt ggtactgaga gtttagatgt tatgaactga gccatcttat gggggacaca 1680 ttcaatcagg atagaccaac agccaatttt ttgtagttgc atggcggttt catacagctc 1740 ctgcatttgt aatagacttt tgctaccttg cactttataa cctccaagtg aatgaacttt 1800 ttggggagtt agtcctatat gagccataac aggtataccc cgggagcaca gttcttcaat 1860 aaatttcatt gcatatttgt ctttggtgta agaaccgact tcaactttga ctgaggtgac 1920 tttactgtcc aatttcatga tatctatggc attcttcaag ccatcagata tgcccgattc 1980 aaacgtaccg aatggcatgt ctaccactac catggaagaa ccttcagctc tgcacacaga 2040 ggcaacatga tatttaaact cattcaagga tagtgtaatt gtgctatcgt accccaatga 2100 agtcatcgcc aaagaatctc cgactaagag taaatcacaa ttagccttgt taacccatgt 2160 tgcagtaatg aagtcatacg cagtacacat cgacaaaggc gtaccagtaa aatatttgtt 2220 cctgatatcc tgaatcgtat tatatttgac aacgtttttt gctgtagaaa aaaatcgctt 2280 agaggaggtg cataattgtc ttttcattat attcattata gttttttctc cttgacgtta 2340 aagtatagag gtatattaac aattttttgt tgatactttt atgacatttg aataagaagt 2400 aatacaaacc gaaaatgttg aaagtattag ttaaagtggt tatgcagctt ttgcatttat 2460 atatctgtta atagatcaaa aatcatcgct tcgctgatta attaccccag aaataaggct 2520 aaaaaactaa tcgcattatt atcctatggt tgttaatttg attcgttgat ttgaaggttt 2580 gtggggccag gttactgcca atttttcctc ttcataacca taaaagctag tattgtagaa 2640 tctttattgt tcggagcagt gcggcgcgag gcacatctgc gtttcaggaa cgcgaccggt 2700 gaagaccagg acgcacggag gagagtcttc cgtcggaggg ctgtcgcccg ctcggcggct 2760 tctaatccgt acttcaatat agcaatgagc agttaagcgt attactgaaa gttccaaaga 2820 gaaggttttt ttaggctaag ataatggggc tctttacatt tccacaacat ataagtaaga 2880 ttagatatgg atatgtatat ggtggtattg ccatgtaata tgattattaa acttctttgc 2940 gtccatccaa aaaaaaagta agaatttttg aaaattcaat ataaatgact gcaccacaca 3000 gaagtactat tcatattctt gggttgggtg ccatgggtac cgttttggca gttgaccttt 3060 taagatttac caatgcccta gttgtgccct tatttagatc acaagaaaga cttgcacaat 3120 ttcagaagac aaatgggaac aacatttcga ttcgcaaact ttatttggaa ggctcgccaa 3180 tattttctta tccagtggag aaatgcgaat gccctgaaac tttctctaaa aaacctattg 3240 acaacttagt cgtcactaca aagacttacc aaaccaaaga agcactagct ccttatttgc 3300 cttatattaa taaaaataca aacttaattt tgattcagaa tggactgggc gttttagaac 3360 tattgagaga agaaatcttt acagattcaa aaaacagacc acatttattt cagggtgtca 3420 tttctcatgg cgtttatcag gataaagcag gagttttcaa tcatgctggg tgggctggta 3480 tgaaaattgc caaattgcct tggactgagg aagaaatgat tcaaaaaaaa tccgtagtcg 3540 aggacgatgc agccaataac tcattagtaa aattattaac agaaccaaaa tttgccaagg 3600 aatttggaat agaacattcc acttatcaag aaatgctctt tggtcaatta tttaaatttt 3660 tggttaatgc atgcatgaat ccagttacgg ctattctaga ttgtgtcaat ggtgagatga 3720 aggccagttg cggtccagtt ttcacatcta ttattgacga gtgtttacaa atattgagag 3780 ttgcttatag gccattgttt caataccacg aaaaatacag cggtaatgaa gaatacccag 3840 aaatggatgt caatgcagta ctgacaaccg ataacatggt ctccgaagtc actagaattg 3900 gctgtgacat caattctagg aacagtagtt caatgagaca agacactttg ttcttaagag 3960 acattgaaat tgagtatatc aatggctatg tcgtcaaatt ggctgataac ttaaatctag 4020 acccaaactg ctgcaaggtt aataaaacta tcggagaatt ggccactatg agattagcat 4080 tgaacaggtc gagaagtatc aatggtgact ggagaaagga ctgatttgcc agcttactat 4140 ccttcttgaa aatatgcact ctatatcttt tagttcttaa ttgcaacaca tagatttgct 4200 gtataacgaa ttttatgcta ttttttaaat ttggagttca gtgataaaag tgtcacagcg 4260 aatttcctca catgtaggga ccgaattgtt tacaagttct ctgtaccacc atggagacat 4320 caaagattga aaatctatgg aaagatatgg acggtagcaa caagaatata gcacgagccg 4380 cgaagttcat ttcgttactt ttgatatcgc tcacaactat tgcgaagcgc ttcagtgaaa 4440 aaatcataag gaaaagttgt aaatattatt ggtagtattc gtttggtaaa gtagaggggg 4500 taatttttcc cctttatttt gttcatacat tcttaaattg ctttgcctct ccttttggaa 4560 agctatactt cggagcactg ttgagcgaag gctcattaga tatattttct gtcattttcc 4620 ttaacccaaa aataagggaa agggtccaaa aagcgctcgg acaactgttg accgtgatcc 4680 gaaggactgg ctatacagtg ttcacaaaat agccaagctg aaaataatgt gtagctatgt 4740 tcagttagtt tggctagcaa agatataaaa gcaggtcgga aatatttatg ggcattatta 4800 tgcagagcat caacatgata aaaaaaaaca gttgaatatt ccctcaaaaa tgaaaatctt 4860 ccatactgtc gaagaagttg ttcaatggag aacacaggag ctgagggaaa ctagatttag 4920 agaaactatt gggttcgttc ccacaatggg ttgcctgcat tcgggtcacg ctagtttgat 4980 ctcgcagtct gtgaaggaaa acacctatac tgtggtcagt atatttgtaa atccctccca 5040 gtttgcgcca acggaagatc tagataacta tcctcgaact ttgccagacg acatcaaatt 5100 gcttgagtcg ttgaaggtgg atgttctatt tgctcctaat gcacacgtga tgtatccaca 5160 gggaattccg ctcgacatag aagagcagaa aggccctttt gttagtgttc ttggattgag 5220 tgaaaaatta gaggggaaga cgagacctaa cttctttagg ggcgtggcaa ctgtcgtgac 5280 taaactattc aatatcgtta tggcggatgt ggcttatttt gggcagaagg acattcaaca 5340 gttcattgtt ttacagtgta tggtggacga actgtttgtt aatacaaggc tacaaatgat 5400 gcctattgta agaaacaata atggactggc tctgagtagt agaaacaaat atctttgtcc 5460 agagtcttta aagatctctg aaaaccttta ccgcgggctg aaagctgcgg aaaatgctat 5520 taggagacta gcaccagggg gacgtctctc cagatcagaa atcatcgata ctgtgactca 5580 aatatgggca ccctacgttg attcccacga tttcaaaatc gactatgttt ccttagcaga 5640 ttttaagact cttgatgaac tctccgatgt tgaaaacacc agcgaacagc agccaatagt 5700 cattagttgt gctgtatacg tgactgaccg cgaaaaaccc gatacggtcg tcagactaat 5760 agataacatc gttatttaag cgaatttctt atgatttatg atttttatta ttaaataagt 5820 tataaaaaaa ataagtgtat acaaatttta aagtgactct taggttttaa aacgaaaatt 5880 cttattcttg agtaactctt tcctgtaggt caggttgctt tctcaggtat agcatgaggt 5940 cgctcttatt gaccacacct ctaccggggt tgcaatttct ttttctatta gtagctaaaa 6000 atgggtcacg tgatctatat tcgaaagggg cggttgcctc aggaaggcac cggcggtctt 6060 tcgtccgtgc ggagatatct gcgccgttca ggggtccatg tgccttggac gatattaagg 6120 cagaaggcag tatcggggcg gatcactccg aaccgagatt agttaagccc ttcccatctc 6180 aagatgggga gcaaatggca ttatactcct gctagaaagt taactgtgca catattctta 6240 aattatacaa cattctggag agctattgtt caaaaaacaa acatttcgca ggctaaaatg 6300 tggagatagg ataagttttg tagacatata taaacaatca gtaattggat tgaaaatttg 6360 gtgttgtgaa ttgctcttca ttatgcacct tattcaatta tcatcaagaa tagtaatagt 6420 taagtaaaca caagattaac ataataaaaa aaataattct ttcataatga ttagaaccat 6480 gttgcaagga aagttacata gggtaaaggt tactcacgcc gatttgcatt atgagggttc 6540 ctgtgctatt gatcaggatt tcctagacgc tgcaggcatt ttagaaaacg aagcgattga 6600 catctggaat gttaccaatg gtaagagatt tagtacatat gccatcgctg cagaaagagg 6660 cagcagaatt atctcagtga atggagctgc cgcgcattgt gcttctgttg gcgatattgt 6720 aattatagca tcattcgtta ctatgccaga cgaagaagct agaacatgga gaccgaatgt 6780 cgcttacttt gaaggtgata atgaaatgaa gagaactgct aaagctatac cagtccaggt 6840 tgcttgaaac taacgctaat cgataaaaca ttagatttca aactagataa ggaccatgta 6900 taagaactat atacttccaa tataatatag tataagcttt aagatagtat ctctcgatct 6960 accgttccac gtgactagtc caaggatttt ttttaacagg tgccccgggt aacccagttc 7020 ctcactattt tttactgcgg aagcggaagc ggaaaatacg gaaacgcgcg ggaacataca 7080 aaacatacaa aatatacctt tctcacacaa gaaatatatg ctacttgcaa aatatcatac 7140 caaaaaactt ttcacaaccg aaaccaaaac caacggatat catacattac actaccacca 7200 ttcaaacttt actactatcc tcccttcagt ttcccttttt ctgccttttt cggtgacgga 7260 aatacgcttc agagacccta aagggaaatc catgccataa caggaaagta acatcccaat 7320 gcggactata ccaccccacc acactcctac caataacggt aactattcta tgttttctta 7380 ctcctatgtc tattcatctt tcatctgact acctaatact atgcaaaaat gtaaaatcat 7440 cacacaaaac ataaacaatc aaaatcagcc atttccgcac cttttcctct gtccactttc 7500 aaccgtccct ccaaatgtaa aatggcctat cggaatacat tttctacatc ctaactacta 7560 taaaacaacc tttagactta cgtttgctac tctcatggtt taaacatcgt cgacctgcag 7620 gcatgcaagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct 7680 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg 7740 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct 7800 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg 7860 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc 7920 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg 7980 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct 8040 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca 8100 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct 8160 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc 8220 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt 8280 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc 8340 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc 8400 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg 8460 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc 8520 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag 8580 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga 8640 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat 8700 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag 8760 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat 8820 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc 8880 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat 8940 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag 9000 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg 9060 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc 9120 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca 9180 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg 9240 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc 9300 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta 9360 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc 9420 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg 9480 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc 9540 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc 9600 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat 9660 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag 9720 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc 9780 ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa 9840 taggcgtatc acgaggccct ttcgtc 9866 <210> 5 <211> 8837 <212> DNA <213> Artificial Sequence <400> 5 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 60 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 120 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 180 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc 240 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat 300 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt 360 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt acccggggat 420 cctctagaga ttgtttaaac tttcctctaa tcaggttcca ccaaacagat accccggtgt 480 ttcacggaat ggtacgtttg atatcgctga tttgagagga ggttacactt gaagaatcac 540 agtcttgcga ccggctattc aacaaggcat tcccccaagt ttgaattctt tgaaatagat 600 tgctattagc tagtaatcca ccaaatcctt cgctgctcac caatggaatc gcaagatgcc 660 cacgatgaga ctgttcaggt taaacgcaaa agaaacacac tctgggaatt tcttcccaaa 720 ttgtatctct caatacgcat caacccatgt caattaaaca cgctgtatag agactaggca 780 gatctgacga tcacctagcg actctctcca ccgtttgacg aggccattta caaaaacata 840 acgaacgaca agcctactcg aattcgtttc caaactcttt tcgaacttgt cttcaactgc 900 tttcgcatga agtacctccc aactactttt cctcacactt gtactccatg actaaacccc 960 ccctcccatt acaaactaaa atcttacttt tattttcttt tgccctctct gtcgctctgc 1020 cttaactacg tatttctcgc cgagaaaaac ttcaatttaa gctattctcc aaaaatctta 1080 gcgtatattt tttttccaaa gtgagcaaat taaagccttc gagcgtccca aaaccttctc 1140 aagcaaggtt ttcagtataa tgttacatgc gtacacgcgt ttgtacagaa aaaaaagaaa 1200 aatttgaaat ataaataacg ttcttaatac taacataact ataaaaaaat aaatagggac 1260 ctagacttca ggttgtctaa ctccttcctt ttcggttaga gcggatgtgg ggggagggcg 1320 tgaatgtaag cgtgacataa ctaattacat gatatcgaca aaggaaaagg ggcctgttta 1380 cttttcgtta attgaagaga ggaattcatt ccataagtcc tccttaactt tgaaagtatg 1440 agtacctctc tcaggaaatg tcctatcctc tacacttgca atgtactcct tcaagccctg 1500 agtggcaata tctgtcatat ttacagcttg cttcacaaat tttgggacag aatcgccttg 1560 catcccgaga aggtcagata taactagaac ttgtccactg gtaccgttac ctgcaccgat 1620 acctattgtt ggtactgaga gtttagatgt tatgaactga gccatcttat gggggacaca 1680 ttcaatcagg atagaccaac agccaatttt ttgtagttgc atggcggttt catacagctc 1740 ctgcatttgt aatagacttt tgctaccttg cactttataa cctccaagtg aatgaacttt 1800 ttggggagtt agtcctatat gagccataac aggtataccc cgggagcaca gttcttcaat 1860 aaatttcatt gcatatttgt ctttggtgta agaaccgact tcaactttga ctgaggtgac 1920 tttactgtcc aatttcatga tatctatggc attcttcaag ccatcagata tgcccgattc 1980 aaacgtaccg aatggcatgt ctaccactac catggaagaa ccttcagctc tgcacacaga 2040 ggcaacatga tatttaaact cattcaagga tagtgtaatt gtgctatcgt accccaatga 2100 agtcatcgcc aaagaatctc cgactaagag taaatcacaa ttagccttgt taacccatgt 2160 tgcagtaatg aagtcatacg cagtacacat cgacaaaggc gtaccagtaa aatatttgtt 2220 cctgatatcc tgaatcgtat tatatttgac aacgtttttt gctgtagaaa aaaatcgctt 2280 agaggaggtg cataattgtc ttttcattat attcattata gttttttctc cttgacgtta 2340 aagtatagag gtatattaac aattttttgt tgatactttt atgacatttg aataagaagt 2400 aatacaaacc gaaaatgttg aaagtattag ttaaagtggt tatgcagctt ttgcatttat 2460 atatctgtta atagatcaaa aatcatcgct tcgctgatta attaccccag aaataaggct 2520 aaaaaactaa tcgcattatt atcctatggt tgttaatttg attcgttgat ttgaaggttt 2580 gtggggccag gttactgcca atttttcctc ttcataacca taaaagctag tattgtagaa 2640 tctttattgt tcggagcagt gcggcgcgag gcacatctgc gtttcaggaa cgcgaccggt 2700 gaagaccagg acgcacggag gagagtcttc cgtcggaggg ctgtcgcccg ctcggcggct 2760 tctaatccgt acttcaatat agcaatgagc agttaagcgt attactgaaa gttccaaaga 2820 gaaggttttt ttaggctaag ataatggggc tctttacatt tccacaacat ataagtaaga 2880 ttagatatgg atatgtatat ggtggtattg ccatgtaata tgattattaa acttctttgc 2940 gtccatccaa aaaaaaagta agaatttttg aaaattcaat ataaatgact gcaccacaca 3000 gaagtactat tcatattctt gggttgggtg ccatgggtac cgttttggca gttgaccttt 3060 taagatttac caatgcccta gttgtgccct tatttagatc acaagaaaga cttgcacaat 3120 ttcagaagac aaatgggaac aacatttcga ttcgcaaact ttatttggaa ggctcgccaa 3180 tattttctta tccagtggag aaatgcgaat gccctgaaac tttctctaaa aaacctattg 3240 acaacttagt cgtcactaca aagacttacc aaaccaaaga agcactagct ccttatttgc 3300 cttatattaa taaaaataca aacttaattt tgattcagaa tggactgggc gttttagaac 3360 tattgagaga agaaatcttt acagattcaa aaaacagacc acatttattt cagggtgtca 3420 tttctcatgg cgtttatcag gataaagcag gagttttcaa tcatgctggg tgggctggta 3480 tgaaaattgc caaattgcct tggactgagg aagaaatgat tcaaaaaaaa tccgtagtcg 3540 aggacgatgc agccaataac tcattagtaa aattattaac agaaccaaaa tttgccaagg 3600 aatttggaat agaacattcc acttatcaag aaatgctctt tggtcaatta tttaaatttt 3660 tggttaatgc atgcatgaat ccagttacgg ctattctaga ttgtgtcaat ggtgagatga 3720 aggccagttg cggtccagtt ttcacatcta ttattgacga gtgtttacaa atattgagag 3780 ttgcttatag gccattgttt caataccacg aaaaatacag cggtaatgaa gaatacccag 3840 aaatggatgt caatgcagta ctgacaaccg ataacatggt ctccgaagtc actagaattg 3900 gctgtgacat caattctagg aacagtagtt caatgagaca agacactttg ttcttaagag 3960 acattgaaat tgagtatatc aatggctatg tcgtcaaatt ggctgataac ttaaatctag 4020 acccaaactg ctgcaaggtt aataaaacta tcggagaatt ggccactatg agattagcat 4080 tgaacaggtc gagaagtatc aatggtgact ggagaaagga ctgatttgcc agcttactat 4140 ccttcttgaa aatatgcact ctatatcttt tagttcttaa ttgcaacaca tagatttgct 4200 gtataacgaa ttttatgcta ttttttaaat ttggagttca gtgataaaag tgtcacagcg 4260 aatttcctca catgtaggga ccgaattgtt tacaagttct ctgtaccacc atggagacat 4320 caaagattga aaatctatgg aaagatatgg acggtagcaa caagaatata gcacgagccg 4380 cgaagttcat ttcgttactt ttgatatcgc tcacaactat tgcgaagcgc ttcagtgaaa 4440 aaatcataag gaaaagttgt aaatattatt ggtagtattc gtttggtaaa gtagaggggg 4500 taatttttcc cctttatttt gttcatacat tcttaaattg ctttgcctct ccttttggaa 4560 agctatactt cggagcactg ttgagcgaag gctcattaga tatattttct gtcattttcc 4620 ttaacccaaa aataagggaa agggtccaaa aagcgctcgg acaactgttg accgtgatcc 4680 gaaggactgg ctatacagtg ttcacaaaat agccaagctg aaaataatgt gtagctatgt 4740 tcagttagtt tggctagcaa agatataaaa gcaggtcgga aatatttatg ggcattatta 4800 tgcagagcat caacatgata aaaaaaaaca gttgaatatt ccctcaaaaa tgaaaatctt 4860 ccatactgtc gaagaagttg ttcaatggag aacacaggag ctgagggaaa ctagatttag 4920 agaaactatt gggttcgttc ccacaatggg ttgcctgcat tcgggtcacg ctagtttgat 4980 ctcgcagtct gtgaaggaaa acacctatac tgtggtcagt atatttgtaa atccctccca 5040 gtttgcgcca acggaagatc tagataacta tcctcgaact ttgccagacg acatcaaatt 5100 gcttgagtcg ttgaaggtgg atgttctatt tgctcctaat gcacacgtga tgtatccaca 5160 gggaattccg ctcgacatag aagagcagaa aggccctttt gttagtgttc ttggattgag 5220 tgaaaaatta gaggggaaga cgagacctaa cttctttagg ggcgtggcaa ctgtcgtgac 5280 taaactattc aatatcgtta tggcggatgt ggcttatttt gggcagaagg acattcaaca 5340 gttcattgtt ttacagtgta tggtggacga actgtttgtt aatacaaggc tacaaatgat 5400 gcctattgta agaaacaata atggactggc tctgagtagt agaaacaaat atctttgtcc 5460 agagtcttta aagatctctg aaaaccttta ccgcgggctg aaagctgcgg aaaatgctat 5520 taggagacta gcaccagggg gacgtctctc cagatcagaa atcatcgata ctgtgactca 5580 aatatgggca ccctacgttg attcccacga tttcaaaatc gactatgttt ccttagcaga 5640 ttttaagact cttgatgaac tctccgatgt tgaaaacacc agcgaacagc agccaatagt 5700 cattagttgt gctgtatacg tgactgaccg cgaaaaaccc gatacggtcg tcagactaat 5760 agataacatc gttatttaag cgaatttctt atgatttatg atttttatta ttaaataagt 5820 tataaaaaaa ataagtgtat acaaatttta aagtgactct taggttttaa aacgaaaatt 5880 cttattcttg agtaactctt tcctgtaggt caggttgctt tctcaggtat agcatgaggt 5940 cgctcttatt gaccacacct ctaccggcag gtgccccggg taacccagtt cctcactatt 6000 ttttactgcg gaagcggaag cggaaaatac ggaaacgcgc gggaacatac aaaacataca 6060 aaatatacct ttctcacaca agaaatatat gctacttgca aaatatcata ccaaaaaact 6120 tttcacaacc gaaaccaaaa ccaacggata tcatacatta cactaccacc attcaaactt 6180 tactactatc ctcccttcag tttccctttt tctgcctttt tcggtgacgg aaatacgctt 6240 cagagaccct aaagggaaat ccatgccata acaggaaagt aacatcccaa tgcggactat 6300 accaccccac cacactccta ccaataacgg taactattct atgttttctt actcctatgt 6360 ctattcatct ttcatctgac tacctaatac tatgcaaaaa tgtaaaatca tcacacaaaa 6420 cataaacaat caaaatcagc catttccgca ccttttcctc tgtccacttt caaccgtccc 6480 tccaaatgta aaatggccta tcggaataca ttttctacat cctaactact ataaaacaac 6540 ctttagactt acgtttgcta ctctcatggt ttaaacatcg tcgacctgca ggcatgcaag 6600 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc 6660 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta 6720 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca 6780 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc 6840 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc 6900 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat 6960 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt 7020 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg 7080 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc 7140 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt 7200 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa 7260 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta 7320 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa 7380 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa 7440 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt 7500 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt 7560 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat 7620 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat 7680 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc 7740 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc 7800 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta 7860 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga 7920 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg 7980 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc 8040 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat 8100 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag 8160 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat 8220 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa 8280 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa 8340 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga 8400 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg 8460 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc 8520 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg 8580 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact 8640 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat 8700 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt 8760 gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat 8820 cacgaggccc tttcgtc 8837 <210> 6 <211> 9575 <212> DNA <213> Artificial Sequence <400> 6 tgatccaata tcaaaggaaa tgatagcatt gaaggatgag actaatccaa ttgaggagtg 60 gcagcatata gaacagctaa agggtagtgc tgaaggaagc atacgatacc ccgcatggaa 120 tgggataata tcacaggagg tactagacta cctttcatcc tacataaata gacgcatata 180 agtacgcatt taagcataaa cacgcactat gccgttcttc tcatgtatat atatatacag 240 gcaacacgca gatataggtg cgacgtgaac agtgagctgt atgtgcgcag ctcgcgttgc 300 attttcggaa gcgctcgttt tcggaaacgc tttgaagttc ctattccgaa gttcctattc 360 tctagaaagt ataggaactt cagagcgctt ttgaaaacca aaagcgctct gaagacgcac 420 tttcaaaaaa ccaaaaacgc accggactgt aacgagctac taaaatattg cgaataccgc 480 ttccacaaac attgctcaaa agtatctctt tgctatatat ctctgtgcta tatccctata 540 taacctaccc atccaccttt cgctccttga acttgcatct aaactcgacc tctacatttt 600 ttatgtttat ctctagtatt actctttaga caaaaaaatt gtagtaagaa ctattcatag 660 agtgaatcga aaacaatacg aaaatgtaaa catttcctat acgtagtata tagagacaaa 720 atagaagaaa ccgttcataa ttttctgacc aatgaagaat catcaacgct atcactttct 780 gttcacaaag tatgcgcaat ccacatcggt atagaatata atcggggatg cctttatctt 840 gaaaaaatgc acccgcagct tcgctagtaa tcagtaaacg cgggaagtgg agtcaggctt 900 tttttatgga agagaaaata gacaccaaag tagccttctt ctaaccttaa cggacctaca 960 gtgcaaaaag ttatcaagag actgcattat agagcgcaca aaggagaaaa aaagtaatct 1020 aagatgcttt gttagaaaaa tagcgctctc gggatgcatt tttgtagaac aaaaaagaag 1080 tatagattct ttgttggtaa aatagcgctc tcgcgttgca tttctgttct gtaaaaatgc 1140 agctcagatt ctttgtttga aaaattagcg ctctcgcgtt gcatttttgt tttacaaaaa 1200 tgaagcacag attcttcgtt ggtaaaatag cgctttcgcg ttgcatttct gttctgtaaa 1260 aatgcagctc agattctttg tttgaaaaat tagcgctctc gcgttgcatt tttgttctac 1320 aaaatgaagc acagatgctt cgttcaggtg gcacttttcg gggaaatgtg cgcggaaccc 1380 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct 1440 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg 1500 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg 1560 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc 1620 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca 1680 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac 1740 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa 1800 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg 1860 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt 1920 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg 1980 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc 2040 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga 2100 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta 2160 ttgctgataa atctggagcc ggtgagcgtg ggagccgcgg tatcattgca gcactggggc 2220 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg 2280 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt 2340 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa 2400 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt 2460 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt 2520 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt 2580 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga 2640 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag 2700 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata 2760 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg 2820 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga 2880 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca 2940 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa 3000 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt 3060 tgtgatgctc gtcagggggg cggagcctat ggaaagcggc cgcacaatct ttgaaaagat 3120 aatgtatgat tatgctttca ctcatattta tacagaaact tgatgttttc tttcgagtat 3180 atacaaggtg attacatgta cgtttgaagt acaactctag attttgtagt gccctcttgg 3240 gctagcggta aaggtgcgca ttttttcaca ccctacaatg ttctgttcaa aagattttgg 3300 tcaaacgctg tagaagtgaa agttggtgcg catgtttcgg cgttcgaaac ttctccgcag 3360 tgaaagataa atgatctcca aagtgacagg tgccccgttt tagagctaga aatagcaagt 3420 taaaataagg ctagtccgtt atcaacttga aaaagtggca ccgagtcggt gctttttttc 3480 ttttttgtcc accaccaaaa tgtcaatttt tttgccattt tctaaatagt tttcttcact 3540 ctacaagaga tatcacgatt gtttgctttc gatattggat ccatctaaag tcatttcaac 3600 tatctaagat gtggttgtga ttgggccatt ttgtgaaagc cagtacgcca gcgtcaatac 3660 actcccgtca attagttgca ccatgtccac aaaatcatat accagtagag ctgagactca 3720 tgcaagtccg gttgcatcga aacttttacg tttaatggat gaaaagaaga ccaatttgtg 3780 tgcttctctt gacgttcgtt cgactgatga gctattgaaa cttgttgaaa cgttgggtcc 3840 atacatttgc cttttgaaaa cacacgttga tatcttggat gatttcagtt atgagggtac 3900 tgtcgttcca ttgaaagcat tggcagagaa atacaagttc ttgatatttg aggacagaaa 3960 attcgccgat atcggtaaca cagtcaaatt acaatataca tcgggcgttt accgtatcgc 4020 agaatggtct gatatcacca acgcccacgg ggttactggt gctggtattg ttgctggctt 4080 gaaacaaggt gcgcaagagg tcaccaaaga accaagggga ttattgatgc ttgctgaatt 4140 gtcttccaag ggttctctag cacacggtga atatactaag ggtaccgttg atattgcaaa 4200 gagtgataaa gatttcgtta ttgggttcat tgctcagaac gatatgggag gaagagaaga 4260 agggtttgat tggctaatca tgaccccagg tgtaggttta gacgacaaag gcgatgcatt 4320 gggtcagcag tacagaaccg tcgacgaagt tgtaagtggt ggatcagata tcatcattgt 4380 tggcagagga cttttcgcca agggtagaga tcctaaggtt gaaggtgaaa gatacagaaa 4440 tgctggatgg gaagcgtacc aaaagagaat cagcgctccc cattaattat acaggaaact 4500 taatagaaca aatcacatat ttaatctaat agccacctgc attggcacgg tgcaacacta 4560 cttcaacttc atcttacaaa aagatcacgt gatctgttgt attgaactga aaattttttg 4620 tttgcttctc tctctctctc tttcattatg tgagatttaa aaaccagaaa ctacatcatc 4680 gaaaaagtct agagttttag agctagaaat agcaagttaa aataaggcta gtccgttatc 4740 aacttgaaaa agtggcaccg agtcggtgct ttttttattt tttgtcacta ttgcatgccg 4800 gtagaggtgt ggtcaataag agcgacctca tgctatacct gagaaagcaa cctgacctac 4860 aggaaagagt tactcaagaa taagaatttt cgttttaaaa cctaagagtc actttaaaat 4920 ttgtatacac ttattttttt tataacttat ttaataataa aaatcataaa tcataagaaa 4980 ttcgcctcga gtcagacctt tctcttcttt tttggagggt cacctcctag ctgactcaaa 5040 tcaatgcgtg tttcataaag accagtgatg gattgatgga taagagtggc atctaaaact 5100 tcttttgtag acgtatatcg tttacgatca attgttgtat caaaatattt aaaagcagcg 5160 ggagctccaa gattcgtcaa cgtaaataaa tgaataatat tttctgcttg ttcacgtatt 5220 ggtttgtctc tatgtttgtt atatgcacta agaactttat ctaaattggc atctgctaaa 5280 ataacacgct tagaaaattc actgatttgc tcaataatct catctaaata atgcttatgc 5340 tgctccacaa acaattgttt ttgttcgtta tcttctggac tacccttcaa cttttcataa 5400 tgactagcta aatataaaaa attcacatat ttgcttggca gagccagctc atttcctttt 5460 tgtaattctc cggcactagc cagcatccgt ttacgaccgt tttctaactc aaaaagacta 5520 tatttaggta gtttaatgat taagtctttt ttaacttcct tatatccttt agcttctaaa 5580 aagtcaatcg gatttttttc aaaggaactt ctttccataa ttgtgatccc tagtaactct 5640 ttaacggatt ttaacttctt cgatttccct ttttccacct tagcaaccac taggactgaa 5700 taagctaccg ttggactatc aaaaccacca tatttttttg gatcccagtc ttttttacga 5760 gcaataagct tgtccgaatt tctttttggt aaaattgact ccttggagaa tccgcctgtc 5820 tgtacttctg ttttcttgac aatattgact tggggcatgg acaatacttt gcgcactgtg 5880 gcaaaatctc gccctttatc ccagacaatt tctccagttt ccccattagt ttcgattaga 5940 gggcgtttgc gaatctctcc atttgcaagt gtaatttctg ttttgaagaa gttcatgata 6000 ttagagtaaa agaaatattt tgcggttgct ttgcctattt cttgctcaga cttagcaatc 6060 attttacgaa catcataaac tttataatca ccatagacaa actccgattc aagttttgga 6120 tatttcttaa tcaaagcagt tccaacgacg gcatttagat acgcatcatg ggcatgatgg 6180 taattgttaa tctcacgtac tttatagaat tggaaatctt ttcggaagtc agaaactaat 6240 ttagatttta aggtaatcac tttaacctct cgaataagtt tatcattttc atcgtattta 6300 gtattcatgc gactatccaa aatttgtgcc acatgcttag tgatttggcg agtttcaacc 6360 aattggcgtt tgataaaacc agctttatca agttcactca aacctccacg ttcagctttc 6420 gttaaattat caaacttacg ttgagtgatt aacttggcgt ttagaagttg tctccaatag 6480 tttttcatct ttttgactac ttcttcactt ggaacgttat ccgatttacc acgattttta 6540 tcagaacgcg ttaagacctt attgtctatt gaatcgtctt taaggaaact ttgtggaaca 6600 atgtgatcga catcataatc acttaaacga ttaatatcta attcttggtc cacatacatg 6660 tctcttccat tttggagata atagagatag agcttttcat tttgcaattg agtattttca 6720 acaggatgct ctttaagaat ctgacttcct aattctttga taccttcttc gattcgtttc 6780 atacgctctc gcgaattttt ctggcccttt tgagttgtct gattttcacg tgccatttca 6840 ataacgatat tttctggctt atgccgcccc attactttga ccaattcatc aacaactttt 6900 acagtctgta aaataccttt tttaatagca gggctaccag ctaaatttgc aatatgttca 6960 tgtaaactat cgccttgtcc agacacttgt gctttttgaa tgtcttcttt aaatgtcaaa 7020 ctatcatcat ggatcagctg cataaaattg cgattggcaa aaccatctga tttcaaaaaa 7080 tctaatattg ttttgccaga ttgcttatcc ctaataccat taatcaattt tcgagacaaa 7140 cgtccccaac cagtataacg gcgacgttta agctgtttca tcaccttatc atcaaagagg 7200 tgagcatatg ttttaagtct ttcctcaatc atctccctat cttcaaataa ggtcaatgtt 7260 aaaacaatat cctctaagat atcttcattt tcttcattat ccaaaaaatc tttatcttta 7320 ataattttta gcaaatcatg gtaggtacct aatgaagcat taaatctatc ttcaactcct 7380 gaaatttcaa cactatcaaa acattctatt tttttgaaat aatcttcttt taattgctta 7440 acggttactt ttcgatttgt tttgaagagt aaatcaacaa tggctttctt ctgttcacct 7500 gaaagaaatg ctggttttcg cattccttca gtaacatatt tgacctttgt caattcgtta 7560 taaaccgtaa aatactcata aagcaaacta tgttttggta gtactttttc atttggaaga 7620 tttttatcaa agtttgtcat gcgttcaata aatgattgag ctgaagcacc tttatcgaca 7680 acttcttcaa aattccatgg ggtaattgtt tcttcagact tccgagtcat ccatgcaaaa 7740 cgactattgc cacgcgccaa tggaccaaca taataaggaa ttcgaaaagt caagattttt 7800 tcaatcttct cacgattgtc ttttaaaaat ggataaaagt cttcttgtct tctcaaaata 7860 gcatgcagct cacccaagtg aatttgatgg gtaatagagc cgttgtcaaa ggtccgttgc 7920 ttgcgcagca aatcttcacg atttagtttc accaataatt cctcagtacc atccattttt 7980 tctaaaattg gtttgataaa tttataaaat tcttcttggc tagctccccc atcaatataa 8040 cctgcatatc cgttttttga ttgatcaaaa aagatttctt tatacttttc tggaagttgt 8100 tgtcgaacta aagcttttaa aagagtcaag tcttgatgat gttcatcgta gcgtttaatc 8160 attgaagctg ataggggagc cttagttatt tcagtattta ctcttaggat atctgaaagt 8220 aaaatagcat ctgataaatt cttagctgcc aaaaacaaat cagcatattg atctccaatt 8280 tgcgccaata aattatctaa atcatcatcg taagtatctt ttgaaagctg taatttagca 8340 tcttctgcca aatcaaaatt tgatttaaaa ttaggggtca aacccaatga caaagcaatg 8400 agattcccaa ataagccatt tttcttctca ccggggagct gagcaatgag attttctaat 8460 cgtcttgatt tactcaatcg tgcagaaaga atcgctttag catctactcc acttgcgtta 8520 atagggtttt cttcaaataa ttgattgtag gtttgtacca actggataaa tagtttgtcc 8580 acatcactat tatcaggatt taaatctccc tcaatcaaaa aatgaccacg aaacttaatc 8640 atatgcgcta aggccaaata gattaagcgc aaatccgctt tataagtaga atctaccaat 8700 ttttttcgca gatgatagat agttggatat ttctcatgat aagcaacttc atctactata 8760 tttccaaaaa taggatgacg ttcatgcttc ttgtcttctt ccaccaaaaa agactcttca 8820 agtcgatgaa agaaactatc atctactttc gccatctcat ttgaaaaaat ctcctgtaga 8880 taacaaatac gattcttccg acgtgtatac cttctacgag ctgtccgttt gagacgagtc 8940 gcttccgctg tctctccact gtcaaataaa agagccccta taagattttt tttgatactg 9000 tggcggtctg tatttcccag aaccttgaac tttttagacg gaaccttata ttcatcagtg 9060 atcaccgccc atccgacgct atttgtgccg atatctaagc ctattgagta tttcttatcg 9120 acctttctct tcttttttgg aggtttgtca tcgtcatctt tataatccat ctagaaaact 9180 tagattagat tgctatgctt tctttctaat gagcaagaag taaaaaaagt tgtaatagaa 9240 caagaaaaat gaaactgaaa cttgagaaat tgaagaccgt ttattaactt aaatatcaat 9300 gggaggtcat cgaaagagaa aaaaatcaaa aaaaaaattt tcaagaaaaa gaaacgtgat 9360 aaaaattttt attgcctttt tcgacgaaga aaaagaaacg aggcggcctc ttttttcttt 9420 tccaaacctt tagtacgggt aattaacgac accctagagg aagaaagagg ggaaatttag 9480 tatgctgtgc ttgggtgttt tgaagtggta cggcgatgcg cggagtccga gaaaatctgg 9540 aagagtaaaa aaggagtaga aacattttga agatc 9575 <210> 7 <211> 11509 <212> DNA <213> Artificial Sequence <400> 7 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 60 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 120 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 120 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 180 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 180 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc 240 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc 240 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat 300 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat 300 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt 360 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt 360 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt acccggggat 420 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt acccggggat 420 cctctagaga ttgtttaaac ctttttttta ttgtttcctg ttgtttttct ctttcttcct 480 cctctagaga ttgtttaaac ctttttttta ttgtttcctg ttgtttttct ctttcttcct 480 tttctaattg ttctttagtt tctttgattc tttgtaatcg atcatcttcc aaggactgtg 540 tttctaattg ttctttagtt tctttgattc tttgtaatcg atcatcttcc aaggactgtg 540 tatttaccac attttgaaat tcgtccagtt tttcttgagt aaaagatgta atttcaaata 600 tatttaccac attttgaaat tcgtccagtt tttcttgagt aaaagatgta atttcaaata 600 taatctcttg gcctcgggag gtgttgtgga ttttcttaaa ttcacttttc agcatttgca 660 taatctcttg gcctcgggag gtgttgtgga ttttcttaaa ttcacttttc agcatttgca 660 attgactatc cattacattt tgtacatttt tgaattctat ttctggagcg gtataaggat 720 attgactatc cattacattt tgtacatttt tgaattctat ttctggagcg gtataaggat 720 acattggggt catcgcaaaa tgtaatgtta tagaagattc aaccggctct ttgtcaacag 780 acattggggt catcgcaaaa tgtaatgtta tagaagattc aaccggctct ttgtcaacag 780 atcgaagcgt aatttcgaat ataatctgtg gctgcttatc ccagctagac tttcttttag 840 ttaagtcagt aaagtcatcc atataaatgg aacgtatcgc ttcaagttcg ttgcactgta 900 tttcataata ttgatctaaa gtgagatgac tcaatgacat ttatacatag tatgtgttaa 960 ggggaacgga aaattattga aaaaaaaaat caatgcttat aaaatcttaa aagacaacga 1020 ggctttccaa caatgtaaag attgaaaatt tagtgtattt aatgtccttt tgtaatgaaa 1080 ataacttctt gtaagaaaga agatagagat aaatgtaaaa gtaaatttgc ttaaaaagat 1140 tctaggctgt ttatagattg attacaccag gttcttatct caaaaatcag ctactcaact 1200 gcttcctaag aaactaccta catcactata gtccaactta aaacaaataa taaacttcta 1260 aggctttcgt gtaaagtgat gttgggattg tccgatgaaa taacaagcag gcccttgcac 1320 gtcaagatta atttgaataa agaagtaaca tgtcatcttt atgtaatcgc tgttattcca 1380 tacagaacaa taaatacgta tatttcgtat gtcatgtgga gtatatattc ttttttattc 1440 cggattagaa gccgccgagc gggtgacagc cctccgaagg aagactctcc tccgtgcgtc 1500 ctcgtcttca ccggtcgcgt tcctgaaacg cagatgtgcc tcgcgccgca ctgctccgaa 1560 caataaagat tctacaatac tagcttttat ggttatgaag aggaaaaatt ggcagtaacc 1620 tggccccaca aaccttcaaa tgaacgaatc aaattaacaa ccataggatg ataatgcgat 1680 tagtttttta gccttatttc tggggtaatt aatcagcgaa gcgatgattt ttgatctatt 1740 aacagatata taaatgcaaa aactgcataa ccactttaac taatactttc aacattttcg 1800 gtttgtatta cttcttattc aaatgtaata aaagtatcaa caaaaaattg ttaatatacc 1860 tctatacttt aacgtcaagg agatgtctat tccagaaact caaaaagcca ttatcttcta 1920 cgaatccaac ggcaagttgg agcataagga tatcccagtt ccaaagccaa agcccaacga 1980 attgttaatc aacgtcaagt actctggtgt ctgccacacc gatttgcacg cttggcatgg 2040 tgactggcca ttgccaacta agttaccatt agttggtggt cacgaaggtg ccggtgtcgt 2100 tgtcggcatg ggtgaaaacg ttaagggctg gaagatcggt gactacgccg gtatcaaatg 2160 gttgaacggt tcttgtatgg cctgtgaata ctgtgaattg ggtaacgaat ccaactgtcc 2220 tcacgctgac ttgtctggtt acacccacga cggttctttc caagaatacg ctaccgctga 2280 cgctgttcaa gccgctcaca ttcctcaagg tactgacttg gctgaagtcg cgccaatctt 2340 gtgtgctggt atcaccgtat acaaggcttt gaagtctgcc aacttgagag caggccactg 2400 ggcggccatt tctggtgctg ctggtggtct aggttctttg gctgttcaat atgctaaggc 2460 gatgggttac agagtcttag gtattgatgg tggtccagga aaggaagaat tgtttacctc 2520 gctcggtggt gaagtattca tcgacttcac caaagagaag gacattgtta gcgcagtcgt 2580 taaggctacc aacggcggtg cccacggtat catcaatgtt tccgtttccg aagccgctat 2640 cgaagcttct accagatact gtagggcgaa cggtactgtt gtcttggttg gtttgccagc 2700 cggtgcaaag tgctcctctg atgtcttcaa ccacgttgtc aagtctatct ccattgtcgg 2760 ctcttacgtg gggaacagag ctgataccag agaagcctta gatttctttg ccagaggtct 2820 agtcaagtct ccaataaagg tagttggctt atccagttta ccagaaattt acgaaaagat 2880 ggagaagggc caaattgctg gtagatacgt tgttgacact tctaaataag cggatctctt 2940 atgtctttac gatttatagt tttcattatc aagtatgcct atattagtat atagcatctt 3000 tagatgacag tgttcgaagt ttcacgaata aaagataata ttctactttt tgctcccacc 3060 gcgtttgcta gcacgagtga acaccatccc tcgcctgtga gttgtaccca ttcctctaaa 3120 ctgtagacat ggtagcttca gcagtgttcg ttatgtacgg catcctccaa caaacagtcg 3180 gttatagttt gtcctgctcc tctgaatcgt ctccctcgat atttctcatg gccaggttac 3240 tgccaatttt tcctcttcat aaccataaaa gctagtattg tagaatcttt attgttcgga 3300 gcagtgcggc gcgaggcaca tctgcgtttc aggaacgcga ccggtgaaga cgaggacgca 3360 cggaggagag tcttccgtcg gagggctgtc gcccgctcgg cggcttctaa tccgtacttc 3420 aatatagcaa tgagcagtta agcgtattac tgaaagttcc aaagagaagg tttttttagg 3480 ctaagataat ggggctcttt acatttccac aacatataag taagattaga tatggatatg 3540 tatatggtgg taatgccatg taatatgatt attaaacttc tttgcgtcca tccaaaaaaa 3600 aagtaagaat ttttgaaaat tcaatataaa tgacaatcaa ggaacataaa gtagtttatg 3660 aagctcacaa cgtaaaggct cttaaggctc ctcaacattt ttacaacagc caacccggca 3720 agggttacgt tactgatatg caacattatc aagaaatgta tcaacaatct atcaatgagc 3780 cagaaaaatt ctttgataag atggctaagg aatacttgca ttgggatgct ccatacacca 3840 aagttcaatc tggttcattg aacaatggtg atgttgcatg gtttttgaac ggtaaattga 3900 atgcatcata caattgtgtt gacagacatg cctttgctaa tcccgacaag ccagctttga 3960 tctatgaagc tgatgacgaa tccgacaaca aaatcatcac atttggtgaa ttactcagaa 4020 aagtttccca aatcgctggt gtcttaaaaa gctggggcgt taagaaaggt gacacagtgg 4080 ctatctattt gccaatgatt ccagaagcgg tcattgctat gttggctgtg gctcgtattg 4140 gtgctattca ctctgttgtc tttgctgggt tctccgctgg ttcgttgaaa gatcgtgtcg 4200 ttgacgctaa ttctaaagtg gtcatcactt gtgatgaagg taaaagaggt ggtaagacca 4260 tcaacactaa aaaaattgtt gacgaaggtt tgaacggagt cgatttggtt tcccgtatct 4320 tggttttcca aagaactggt actgaaggta ttccaatgaa ggccggtaga gattactggt 4380 ggcatgagga ggccgctaag cagagaactt acctacctcc tgtttcatgt gacgctgaag 4440 atcctctatt tttattatac acttccggtt ccactggttc tccaaagggt gtcgttcaca 4500 ctacaggtgg ttatttatta ggtgccgctt taacaactag atacgttttt gatattcacc 4560 cagaagatgt tctcttcact gccggtgacg tcggctggat cacgggtcac acctatgctc 4620 tatatggtcc attaaccttg ggtaccgcct caataatttt cgaatccact cctgcctacc 4680 cagattatgg tagatattgg agaattatcc aacgtcacaa ggctacccat ttctatgtgg 4740 ctccaactgc tttaagatta atcaaacgtg taggtgaagc cgaaattgcc aaatatgaca 4800 cttcctcatt acgtgtcttg ggttccgtcg gtgaaccaat ctctccagac ttatgggaat 4860 ggtatcatga aaaagtgggt aacaaaaact gtgtcatttg tgacactatg tggcaaacag 4920 agtctggttc tcatttaatt gctcctttgg caggtgctgt cccaacaaaa cctggttctg 4980 ctaccgtgcc attctttggt attaacgctt gtatcattga ccctgttaca ggtgtggaat 5040 tagaaggtaa tgatgtcgaa ggtgtccttg ccgttaaatc accatggcca tcaatggcta 5100 gatctgtttg gaaccaccac gaccgttaca tggatactta cttgaaacct tatcctggtc 5160 actatttcac aggtgatggt gctggtagag atcatgatgg ttactactgg atcaggggta 5220 gagttgacga cgttgtaaat gtttccggtc atagattatc cacatcagaa attgaagcat 5280 ctatctcaaa tcacgaaaac gtctcggaag ctgctgttgt cggtattcca gatgaattga 5340 ccggtcaaac cgtcgttgca tatgtttccc taaaagatgg ttatctacaa aacaacgcta 5400 ctgaaggtga tgcagaacac atcacaccag ataatttacg tagagaattg atcttacaag 5460 ttaggggtga gattggtcct ttcgcctcac caaaaaccat tattctagtt agagatctac 5520 caagaacaag gtcaggaaag attatgagaa gagttctaag aaaggttgct tctaacgaag 5580 ccgaacagct aggtgaccta actactttgg ccaacccaga agttgtacct gccatcattt 5640 ctgctgtaga gaaccaattt ttctctcaaa aaaagaaata atttgccagc ttactatcct 5700 tcttgaaaat atgcactcta tatcttttag ttcttaattg caacacatag atttgctgta 5760 taacgaattt tatgctattt tttaaatttg gagttcagtg ataaaagtgt cacagcgaat 5820 ttcctcacat gtagggaccg aattgtttac aagttctctg taccaccatg gagacatcaa 5880 aaattgaaaa tctatggaaa gatatggacg gtagcaacaa gaatatagca cgagccgcgg 5940 agttcatttc gttacttttg atatcactca caactattgc gaagcgcttc agtgaaaaaa 6000 tcataaggaa aagttgtaaa tattattggt agtattcgtt tggtaaagta gagggggtaa 6060 tttttcccct ttattttgtt catacattct taaattgctt tgcctctcct tttggaaagc 6120 tatacttcgg agcactgttg agcgaaggct cattagatat attttctgtc attttcctta 6180 acccaaaaat aagggaaagg gtccaaaaag cgctcggaca actgttgacc gtgatccgaa 6240 ggactggcta tacagtgttc acaaaatagc caagctgaaa ataatgtgta gctatgttca 6300 gttagtttgg ctagcaaaga tataaaagca ggtcggaaat atttatgggc attattatgc 6360 agagcatcaa catgataaaa aaaaacagtt gaatattccc tcaaaaatga ctaagctaca 6420 ctttgacact gctgaaccag tcaagatcac acttccaaat ggtttgacat acgagcaacc 6480 aaccggtcta ttcattaaca acaagtttat gaaagctcaa gacggtaaga cctatcccgt 6540 cgaagatcct tccactgaaa acaccgtttg tgaggtctct tctgccacca ctgaagatgt 6600 tgaatatgct atcgaatgtg ccgaccgtgc tttccacgac actgaatggg ctacccaaga 6660 cccaagagaa agaggccgtc tactaagtaa gttggctgac gaattggaaa gccaaattga 6720 cttggtttct tccattgaag ctttggacaa tggtaaaact ttggccttag cccgtgggga 6780 tgttaccatt gcaatcaact gtctaagaga tgctgctgcc tatgccgaca aagtcaacgg 6840 tagaacaatc aacaccggtg acggctacat gaacttcacc accttagagc caatcggtgt 6900 ctgtggtcaa attattccat ggaactttcc aataatgatg ttggcttgga agatcgcccc 6960 agcattggcc atgggtaacg tctgtatctt gaaacccgct gctgtcacac ctttaaatgc 7020 cctatacttt gcttctttat gtaagaaggt tggtattcca gctggtgtcg tcaacatcgt 7080 tccaggtcct ggtagaactg ttggtgctgc tttgaccaac gacccaagaa tcagaaagct 7140 ggcttttacc ggttctacag aagtcggtaa gagtgttgct gtcgactctt ctgaatctaa 7200 cttgaagaaa atcactttgg aactaggtgg taagtccgcc catttggtct ttgacgatgc 7260 taacattaag aagactttac caaatctagt aaacggtatt ttcaagaacg ctggtcaaat 7320 ttgttcctct ggttctagaa tttacgttca agaaggtatt tacgacgaac tattggctgc 7380 tttcaaggct tacttggaaa ccgaaatcaa agttggtaat ccatttgaca aggctaactt 7440 ccaaggtgct atcactaacc gtcaacaatt cgacacaatt atgaactaca tcgatatcgg 7500 taagaaagaa ggcgccaaga tcttaactgg tggcgaaaaa gttggtgaca agggttactt 7560 catcagacca accgttttct acgatgttaa tgaagacatg agaattgtta aggaagaaat 7620 ttttggacca gttgtcactg tcgcaaagtt caagacttta gaagaaggtg tcgaaatggc 7680 taacagctct gaattcggtc taggttctgg tatcgaaaca gaatctttga gcacaggttt 7740 gaaggtggcc aagatgttga aggccggtac cgtctggatc aacacataca acgattttga 7800 ctccagagtt ccattcggtg gtgttaagca atctggttac ggtagagaaa tgggtgaaga 7860 agtctaccat gcatacactg aagtaaaagc tgtcagaatt aagttgtaaa aaaaaccccg 7920 gattctagaa ctagtggatc ccccgggctg caggaattcg atatcaagct tatcgatacc 7980 gtcgacctcg agtcatgtaa ttagttatgt cacgcttaca ttcacgccct ccccccacat 8040 ccgctctaac cgaaaaggaa ggagttagac aacctgaagt ctaggtccct atttattttt 8100 ttatagttat gttagtatta agaacgttat ttatatttca aatttttctt ttttttctgt 8160 acagacgcgt gtacgcatgt aacattatac tgaaaacctt gcttgagaag gttttgggac 8220 gctcgaaggc tttaatttgc tttggtcgtt tgctatgatt taattctgat tctttgcctt 8280 taacaattga taacgtgccc tttattgaac tgctaactac tcgaaaaatt ggacacaatt 8340 cagtttatag caggtgtcct ttaagtgatg tttgctgatt tccgaactgc gcgtatgtta 8400 gtggacccta gataacagtt tattccctaa cgtttctaga taatatatga caaaaatata 8460 caaccgatcg acaaattatt tctaaaatac aaaaaagtta gtcgtgaggt tatccaacgt 8520 tgggtttcta tctatatata aaagctaatc accaaacaat tgagaatcaa cgccgataac 8580 atgatgtaca attatagcag tatacgccag gcgtgctgca tttattcttt accctgtcat 8640 cgttgacgaa tacttttttt taaagggatc gcactctaac cacctgaaag gtactatctg 8700 aaaaagtgtt gactaaaaac agaagcgcct taataatagt gcactactgg aaaactatat 8760 ttaatgtaaa aaagaaacta cattgaatga aatcgaaaag tataatttct ctccgtacct 8820 gtgactgata caatgtaacg atgtagacta agaataagat tctcacttaa aagctgctat 8880 ttcaactgat tatctgcaga gtatgcgatc acaagacaga tcacaactgt gtttatcgga 8940 cataatgcta tatttttgct gcgccagaaa tcgagtacta gtttttaaat atttgttcta 9000 aacggtcaaa cttttcgtaa aaaatgctat aagccgtgtt gtccctttat gaagagctgc 9060 ttcttcactt gtaaatagcg aacaaataaa gaaaacaatc tgctacctct ttgttaaagt 9120 tgaccgcctt gaagagaact gcaagtctct gaaagtttta gctgaatatt gtcacctctt 9180 ttaggattaa cagtaagcat atttttttct ttgagattcg gaagtaaaat accctcggcg 9240 gtttaaacat cgtcgacctg caggcatgca agcttggcgt aatcatggtc atagctgttt 9300 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag 9360 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg 9420 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg 9480 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc 9540 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc 9600 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg 9660 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat 9720 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag 9780 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga 9840 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg 9900 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt 9960 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac 10020 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc 10080 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt 10140 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc 10200 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc 10260 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg 10320 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag 10380 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg 10440 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt 10500 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca 10560 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca 10620 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc 10680 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt 10740 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg 10800 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc 10860 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg 10920 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga 10980 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga 11040 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta 11100 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg 11160 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact 11220 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata 11280 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt 11340 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa 11400 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt 11460 atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtc 11509 <210> 8 <211> 7257 <212> DNA <213> Artificial Sequence <400> 8 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 60 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 120 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 180 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc 240 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat 300 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt 360 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt acccggggat 420 cctctagaga ttgtttaaac tatcatccac tgcacccact attgtgctgt cagaattagg 480 catcccagac ctcgtggtcg gcgcttccgg cggctcaaga atcactacga gtgttttaca 540 aacaattgtg aggacgtact ggtataacat gcctatattg gaaacgatag cctatccacg 600 tatacaccac cagttactgc ccgaccgcat agaactggaa agcttcccca tgatcggtaa 660 agccgtacta agcactctca aggaaatggg atatacgatg aaagaagtat ttcccaagag 720 cgtagttaat gctatacgca atgtcagggg agaatggcat gcggtgagtg actattggag 780 aaaaagagga atctcctccg tatactaaga tgtggggtaa gaatggtact gtaatatagt 840 tcaagataat agtacgtaat gtagggagcc tgcttctgtg gtatataaat aaatactggg 900 cttgcgttta tttttaaaga atcaatattt acttttcatt aacaaagggc atacgccctt 960 aaaacaaaac aaaaaatcca gatcatgcga gctttgcctt tttcagtaag tttccctacc 1020 ttgcagccga gacgcgactt cggaacgatg ccagcatacg tatttaccat tattaaaaaa 1080 atctgatggc ttgatactgc gcgtttcttt ttatagcatt aatacgcaca catacgcgca 1140 ttgttgttta ctataaataa accgtgggga cctgacccag tcatcctccc tgtgtttaca 1200 gtgcggtgca cacgagcctg agacaagccc gtaaccaggc gcgttgccgc aaatacagag 1260 gcgccccaga caacaccgca gtgtgaagca ctgtcaattt aaaaccgtgg cttgctggag 1320 atgcccagac caaccctgtt gggtttttct ctcgagcacg ccgttataat tttagcgtgt 1380 tccgtacctg tgtgcacatc aataagcggt gtaacaaact tgaacttgcc atctcatatc 1440 gtcatatgag cagttgcaga gaaaggcact ttaaataaaa aggcgtggat gataaaaaat 1500 gtatataagt tggatggatt tttgggaaaa agtaatgttt ttgcagacgt tttaaatact 1560 ccctcccttt tcttagtaat ttttattatg tattgactaa gtcaaaaata actatagaaa 1620 actaaagttt acgagaggac ccaaaagttt tgaataacac gtgcctttga ttttttgttt 1680 actttctttt tctagttaat taccaactaa acggattaga agccgccgag cgggtgacag 1740 ccctccgaag gaagactctc ctccgtgcgt cctcgtcttc accggtcgcg ttcctgaaac 1800 gcagatgtgc ctcgcgccgc actgctccga acaataaaga ttctacaata ctagctttta 1860 tggttatgaa gaggaaaaat tggcagtaac ctggccccac aaaccttcaa atgaacgaat 1920 caaattaaca accataggat gataatgcga ttagtttttt agccttattt ctggggtaat 1980 taatcagcga agcgatgatt tttgatctat taacagatat ataaatgcaa aaactgcata 2040 accactttaa ctaatacttt caacattttc ggtttgtatt acttcttatt caaatgtaat 2100 aaaagtatca acaaaaaatt gttaatatac ctctatactt taacgtcaag gagatgtttg 2160 tcagggttaa attgaataaa ccagtaaaat ggtataggtt ctatagtacg ttggattcac 2220 attccctaaa gttacagagc ggctcgaagt ttgtaaaaat aaagccagta aataacttga 2280 ggagtagttc atcagcagat ttcgtgtccc caccaaattc caaattacaa tctttaatct 2340 ggcagaaccc tttacaaaat gtttatataa ctaaaaaacc atggactcca tccacaagag 2400 aagcgatggt tgaattcata actcatttac atgagtcata ccccgaggtg aacgtcattg 2460 ttcaacccga tgtggcagaa gaaatttccc aggatttcaa atctcctttg gagaatgatc 2520 ccaaccgacc tcatatactt tatactggtc ctgaacaaga tatcgtaaac agaacagact 2580 tattggtgac attgggaggt gatgggacta ttttacacgg cgtatcaatg ttcggaaata 2640 cgcaagttcc tccggtttta gcatttgctc tgggcactct gggctttcta ttaccgtttg 2700 attttaagga gcataaaaag gtctttcagg aagtaatcag ctctagagcc aaatgtttgc 2760 atagaacacg gctagaatgt catttgaaaa aaaaggatag caactcatct attgtgaccc 2820 atgctatgaa tgacatattc ttacataggg gtaattcccc tcatctcact aacctggaca 2880 ttttcattga tggggaattt ttgacaagaa cgacagcaga tggtgttgca ttggccactc 2940 caacgggttc cacagcatat tcattatcag caggtggatc tattgtttcc ccattagtcc 3000 ctgctatttt aatgacacca atttgtcctc gctctttgtc attccgacca ctgattttgc 3060 ctcattcatc ccacattagg ataaagatag gttccaaatt gaaccaaaaa ccagtcaaca 3120 gtgtggtaaa actttctgtt gatggtattc ctcaacagga tttagatgtt ggtgatgaaa 3180 tttatgttat aaatgaggtc ggcactatat acatagatgg tactcagctt ccgacgacaa 3240 gaaaaactga aaatgacttt aataattcaa aaaagcctaa aaggtcaggg atttattgtg 3300 tcgccaagac cgagaatgac tggattagag gaatcaatga acttttagga ttcaattcta 3360 gctttaggct gaccaagaga cagactgata atgattaaaa aaaaccccgg attctagaac 3420 tagtggatcc cccgggctgc aggaattcga tatcaagctt atcgataccg tcgacctcga 3480 gtcatgtaat tagttatgtc acgcttacat tcacgccctc cccccacatc cgctctaacc 3540 gaaaaggaag gagttagaca acctgaagtc taggtcccta tttatttttt tatagttatg 3600 ttagtattaa gaacgttatt tatatttcaa atttttcttt tttttctgta cagacgcgtg 3660 tacgcatgta acattatact gaaaaccttg cttgagaagg ttttgggacg ctcgaaggct 3720 ttaatttgcg tcgccctcag tccgctcatt ttagctgaat tttctaatgt tatttttcat 3780 cagcaaaact taacagaacg ttaatttatc tacccctttt agttcattat ctctttttta 3840 tccaacattt tacagagatc tctcacttaa gtctaagtaa agacattatt ttatatggta 3900 cacttataga atatacgata ataataataa aaactatgta acataacctt cagaatttaa 3960 tattagtttc ctttttacct cattgcacta ataaaaaaat tctacagaat ctccgaaaaa 4020 gaaaatccag cttactcttt ttgttttctt cttcacacgt gagcttttcc gccggcatac 4080 gttccgttcc gtgtcgtctt gcataaaatt tccgaatcac atgttcgtaa aacaaccgga 4140 agtgccccga atataaagtc aattctcacc gctgttgtaa ctggagcttt aaggtgttat 4200 ctaaggaagg ataaaagaac ttaaacaaca acaaacaaaa aattaagtta caatgccagc 4260 attattaaaa agattattgt ttcaagtggg tcctcatcca aacgaaagaa cattcacctt 4320 atcctctgtt tcaactgatg ggcactatat ttccttgaga ccatttgtta agccaagcgg 4380 tgatgagtta tcttttcctt tcgaatgggc ctttgccggt acaaacgaaa cagttaaagc 4440 taatgatcaa ggaaacggtg tcgttactca agatttcaat ttctggttgg atacaaatgt 4500 gtacttgaac gttccaaaca cccatcgtgg cgaagtgaac accacttgga aaaattggga 4560 ttctggttgt gtcgaggaaa caggcgctgt ttacccattc ggtgccgaca aagaaagcgt 4620 ctctttcaga gaattgtggc aaccagttga cccatcaaga gaagatctag tcatcgtctc 4680 accaaacaat gagaagttct cgtcgaatgc taggtcaatt gtcctcaaag ttactgacga 4740 agcttatgat ggtttggtta ttgttattgg tagatggatt caagggtttt tgtcccaaaa 4800 gaataataac actattgaag gcttgaactt catcagatta cttgaaaaag attcaggtaa 4860 atctgagttc ttattaagct acggtaagga agtaaacaaa attccacaaa gctacgaaaa 4920 tttgaagaaa ggttccactg taaccagcaa tgggttgaac tgggaagtta ttgaatatca 4980 cgcttaatgt ttaaacatcg tcgacctgca ggcatgcaag cttggcgtaa tcatggtcat 5040 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa 5100 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc 5160 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc 5220 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact 5280 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac 5340 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa 5400 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg 5460 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa 5520 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc 5580 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac 5640 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac 5700 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg 5760 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt 5820 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa 5880 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct 5940 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga 6000 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg 6060 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct 6120 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt 6180 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc 6240 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg 6300 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag 6360 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt 6420 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag 6480 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt 6540 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca 6600 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg 6660 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat 6720 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta 6780 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca 6840 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct 6900 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat 6960 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa 7020 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt 7080 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa 7140 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa 7200 ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtc 7257 <210> 9 <211> 6581 <212> DNA <213> Artificial Sequence <400> 9 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 60 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 120 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 180 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc 240 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat 300 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt 360 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt acccggggat 420 cctctagaga ttgtttaaac tttcctctaa tcaggttcca ccaaacagat accccggtgt 480 ttcacggaat ggtacgtttg atatcgctga tttgagagga ggttacactt gaagaatcac 540 agtcttgcga ccggctattc aacaaggcat tcccccaagt ttgaattctt tgaaatagat 600 tgctattagc tagtaatcca ccaaatcctt cgctgctcac caatggaatc gcaagatgcc 660 cacgatgaga ctgttcaggt taaacgcaaa agaaacacac tctgggaatt tcttcccaaa 720 ttgtatctct caatacgcat caacccatgt caattaaaca cgctgtatag agactaggca 780 gatctgacga tcacctagcg actctctcca ccgtttgacg aggccattta caaaaacata 840 acgaacgaca agcctactcg aattcgtttc caaactcttt tcgaacttgt cttcaactgc 900 tttcgcatga agtacctccc aactactttt cctcacactt gtactccatg actaaacccc 960 ccctcccatt acaaactaaa atcttacttt tattttcttt tgccctctct gtcgctctgc 1020 cttaactacg tatttctcgc cgagaaaaac ttcaatttaa gctattctcc aaaaatctta 1080 gcgtatattt tttttccaaa gtgatgcagg tctcatctgg aatataattc ccccctcctg 1140 aagcaaattt ttcctttgag ccggaatttt tgatattccg agttcttttt ttccattcgc 1200 ggaggttatt ccattcctaa acgagtggcc acaatgaaac ttcaattcat atcgaccgac 1260 tatttttctc cgaaccaaaa aaatagcagg gcgagattgg agctgcggaa aaaagaggaa 1320 aaaatttttt cgtagttttc ttgtgcaaat tagggtgtaa ggtttctagg gcttattggt 1380 tcaagcagaa gagacaacaa ttgtaggtcc taaattcaag gcggatgtaa ggagtattgg 1440 tttcgaaagt ttttccgaag cggcatggca gggactactt gcgcatgcgc tcggattatc 1500 ttcatttttg cttgcaaaaa cgtagaatca tggtaaatta catgaagaat tctctttttt 1560 tttttttttt tttttttttt acctctaaag agtgttgacc aactgaaaaa acccttcttc 1620 aagagagtta aactaagact aaccatcata acttccaagg aattaatcga tatcttgcac 1680 tcctgatttt tcttcaaaga gacagcgcaa aggattatga cactgttgca ttgagtcaaa 1740 agtttttccg aagtgaccca gtgctctttt tttttttccg tgaaggactg acaaatatgc 1800 gcacaagatc caatacgtaa tggaaattcg gaaaaactag gaagaaatgc tgcagggcat 1860 tgccgtgccg atcttttgtc tttcagatat atgagaaaaa gaatattcat caagtgctga 1920 tagaagaata ccactcatat gacgtgggca gaagacagca aacgtaaaca tgagctgctg 1980 cgacatttga tggcttttat ccgacaagcc aggaaactcc accattatct aatgtagcaa 2040 aatatttctt aacacccgaa gttgcgtgtc cccctcacgt ttttaatcat ttgaattagt 2100 atattgaaat tatatataaa ggcaacaatg tccccataat caattccatc tggggtctca 2160 tgttctttcc ccaccttaaa atctataaag atatcataat cgtcaactag ttgatatacg 2220 taaaatcatg ggaaagctat tacaattggc attgcatccg gtcgagatga aggcagcttt 2280 gaagctgaag ttttgcagaa caccgctatt ctccatctat gatcagtcca cgtctccata 2340 tctcttgcac tgtttcgaac tgttgaactt gacctccaga tcgtttgctg ctgtgatcag 2400 agagctgcat ccagaattga gaaactgtgt tactctcttt tatttgattt taagggcttt 2460 ggataccatc gaagacgata tgtccatcga acacgatttg aaaattgact tgttgcgtca 2520 cttccacgag aaattgttgt taactaaatg gagtttcgac ggaaatgccc ccgatgtgaa 2580 ggacagagcc gttttgacag atttcgaatc gattcttatt gaattccaca aattgaaacc 2640 agaatatcaa gaagtcatca aggagatcac cgagaaaatg ggtaatggta tggccgacta 2700 catcttagat gaaaattaca acttgaatgg gttgcaaacc gtccacgact acgacgtgta 2760 ctgtcactac gtagctggtt tggtcggtga tggtttgacc cgtttgattg tcattgccaa 2820 gtttgccaac gaatctttgt attctaatga gcaattgtat gaaagcatgg gtcttttcct 2880 acaaaaaacc aacatcatca gagattacaa tgaagatttg gtcgatggta gatccttctg 2940 gcccaaggaa atctggtcac aatacgctcc tcagttgaag gacttcatga aacctgaaaa 3000 cgaacaactg gggttggact gtataaacca cctcgtctta aacgcattga gtcatgttat 3060 cgatgtgttg acttatttgg ccggtatcca cgagcaatcc actttccaat tttgtgccat 3120 tccccaagtt atggccattg caaccttggc tttggtattc aacaaccgtg aagtgctaca 3180 tggcaatgta aagattcgta agggtactac ctgctattta attttgaaat caaggacttt 3240 gcgtggctgt gtcgagattt ttgactatta cttacgtgat atcaaatcta aattggctgt 3300 gcaagatcca aatttcttaa aattgaacat tcaaatctcc aagatcgaac agtttatgga 3360 agaaatgtac caggataaat tacctcctaa cgtgaagcca aatgaaactc caattttctt 3420 gaaagttaaa gaaagatcca gatacgatga tgaattggtt ccaacccaac aagaagaaga 3480 gtacaagttc aatatggttt tatctatcat cttgtccgtt cttcttgggt tttattatat 3540 atacacttta cacagagcgt gaaactaacg ctaatcgata aaacattaga tttcaaacta 3600 gataaggacc atgtataaga actatatact tccaatataa tatagtataa gctttaagat 3660 agtatctctc gatctaccgt tccacgtgac tagtccaagg atttttttta acaggtgccc 3720 cgggtaaccc agttcctcac tattttttac tgcggaagcg gaagcggaaa atacggaaac 3780 gcgcgggaac atacaaaaca tacaaaatat acctttctca cacaagaaat atatgctact 3840 tgcaaaatat cataccaaaa aacttttcac aaccgaaacc aaaaccaacg gatatcatac 3900 attacactac caccattcaa actttactac tatcctccct tcagtttccc tttttctgcc 3960 tttttcggtg acggaaatac gcttcagaga ccctaaaggg aaatccatgc cataacagga 4020 aagtaacatc ccaatgcgga ctataccacc ccaccacact cctaccaata acggtaacta 4080 ttctatgttt tcttactcct atgtctattc atctttcatc tgactaccta atactatgca 4140 aaaatgtaaa atcatcacac aaaacataaa caatcaaaat cagccatttc cgcacctttt 4200 cctctgtcca ctttcaaccg tccctccaaa tgtaaaatgg cctatcggaa tacattttct 4260 acatcctaac tactataaaa caacctttag acttacgttt gctactctca tggtttaaac 4320 atcgtcgacc tgcaggcatg caagcttggc gtaatcatgg tcatagctgt ttcctgtgtg 4380 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc 4440 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt 4500 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg 4560 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt 4620 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc 4680 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa 4740 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa 4800 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc 4860 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc 4920 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag 4980 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga 5040 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc 5100 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac 5160 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg 5220 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca 5280 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa 5340 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa 5400 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt 5460 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag 5520 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat 5580 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc 5640 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa 5700 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca 5760 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa 5820 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt 5880 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc 5940 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact 6000 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc 6060 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg 6120 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct 6180 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc 6240 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag 6300 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac 6360 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg 6420 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt 6480 tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac 6540 attaacctat aaaaataggc gtatcacgag gccctttcgt c 6581 <210> 10 <211> 9748 <212> DNA <213> Artificial Sequence <400> 10 tgatccaata tcaaaggaaa tgatagcatt gaaggatgag actaatccaa ttgaggagtg 60 gcagcatata gaacagctaa agggtagtgc tgaaggaagc atacgatacc ccgcatggaa 120 tgggataata tcacaggagg tactagacta cctttcatcc tacataaata gacgcatata 180 agtacgcatt taagcataaa cacgcactat gccgttcttc tcatgtatat atatatacag 240 gcaacacgca gatataggtg cgacgtgaac agtgagctgt atgtgcgcag ctcgcgttgc 300 attttcggaa gcgctcgttt tcggaaacgc tttgaagttc ctattccgaa gttcctattc 360 tctagaaagt ataggaactt cagagcgctt ttgaaaacca aaagcgctct gaagacgcac 420 tttcaaaaaa ccaaaaacgc accggactgt aacgagctac taaaatattg cgaataccgc 480 ttccacaaac attgctcaaa agtatctctt tgctatatat ctctgtgcta tatccctata 540 taacctaccc atccaccttt cgctccttga acttgcatct aaactcgacc tctacatttt 600 ttatgtttat ctctagtatt actctttaga caaaaaaatt gtagtaagaa ctattcatag 660 agtgaatcga aaacaatacg aaaatgtaaa catttcctat acgtagtata tagagacaaa 720 atagaagaaa ccgttcataa ttttctgacc aatgaagaat catcaacgct atcactttct 780 gttcacaaag tatgcgcaat ccacatcggt atagaatata atcggggatg cctttatctt 840 gaaaaaatgc acccgcagct tcgctagtaa tcagtaaacg cgggaagtgg agtcaggctt 900 tttttatgga agagaaaata gacaccaaag tagccttctt ctaaccttaa cggacctaca 960 gtgcaaaaag ttatcaagag actgcattat agagcgcaca aaggagaaaa aaagtaatct 1020 aagatgcttt gttagaaaaa tagcgctctc gggatgcatt tttgtagaac aaaaaagaag 1080 tatagattct ttgttggtaa aatagcgctc tcgcgttgca tttctgttct gtaaaaatgc 1140 agctcagatt ctttgtttga aaaattagcg ctctcgcgtt gcatttttgt tttacaaaaa 1200 tgaagcacag attcttcgtt ggtaaaatag cgctttcgcg ttgcatttct gttctgtaaa 1260 aatgcagctc agattctttg tttgaaaaat tagcgctctc gcgttgcatt tttgttctac 1320 aaaatgaagc acagatgctt cgttcaggtg gcacttttcg gggaaatgtg cgcggaaccc 1380 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct 1440 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg 1500 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg 1560 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc 1620 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca 1680 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac 1740 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa 1800 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg 1860 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt 1920 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg 1980 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc 2040 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga 2100 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta 2160 ttgctgataa atctggagcc ggtgagcgtg ggagccgcgg tatcattgca gcactggggc 2220 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg 2280 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt 2340 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa 2400 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt 2460 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt 2520 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt 2580 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga 2640 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag 2700 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata 2760 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg 2820 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga 2880 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca 2940 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa 3000 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt 3060 tgtgatgctc gtcagggggg cggagcctat ggaaagcggc cgcacaatct ttgaaaagat 3120 aatgtatgat tatgctttca ctcatattta tacagaaact tgatgttttc tttcgagtat 3180 atacaaggtg attacatgta cgtttgaagt acaactctag attttgtagt gccctcttgg 3240 gctagcggta aaggtgcgca ttttttcaca ccctacaatg ttctgttcaa aagattttgg 3300 tcaaacgctg tagaagtgaa agttggtgcg catgtttcgg cgttcgaaac ttctccgcag 3360 tgaaagataa atgatcatca atggtttgaa cggaaggttt tagagctaga aatagcaagt 3420 taaaataagg ctagtccgtt atcaacttga aaaagtggca ccgagtcggt gcaaacaagc 3480 gcaagtggtt tagtggtaaa atccaacgtt gccatcgttg ggcccccggt tcgattccgg 3540 gcttgcgcac atattgaaaa aatactctgg ttttagagct agaaatagca agttaaaata 3600 aggctagtcc gttatcaact tgaaaaagtg gcaccgagtc ggtgcttttt ttcttttttg 3660 tccaccacca aaatgtcaat ttttttgcca ttttctaaat agttttcttc actctacaag 3720 agatatcacg attgtttgct ttcgatattg gatccatcta aagtcatttc aactatctaa 3780 gatgtggttg tgattgggcc attttgtgaa agccagtacg ccagcgtcaa tacactcccg 3840 tcaattagtt gcaccatgtc cacaaaatca tataccagta gagctgagac tcatgcaagt 3900 ccggttgcat cgaaactttt acgtttaatg gatgaaaaga agaccaattt gtgtgcttct 3960 cttgacgttc gttcgactga tgagctattg aaacttgttg aaacgttggg tccatacatt 4020 tgccttttga aaacacacgt tgatatcttg gatgatttca gttatgaggg tactgtcgtt 4080 ccattgaaag cattggcaga gaaatacaag ttcttgatat ttgaggacag aaaattcgcc 4140 gatatcggta acacagtcaa attacaatat acatcgggcg tttaccgtat cgcagaatgg 4200 tctgatatca ccaacgccca cggggttact ggtgctggta ttgttgctgg cttgaaacaa 4260 ggtgcgcaag aggtcaccaa agaaccaagg ggattattga tgcttgctga attgtcttcc 4320 aagggttctc tagcacacgg tgaatatact aagggtaccg ttgatattgc aaagagtgat 4380 aaagatttcg ttattgggtt cattgctcag aacgatatgg gaggaagaga agaagggttt 4440 gattggctaa tcatgacccc aggtgtaggt ttagacgaca aaggcgatgc attgggtcag 4500 cagtacagaa ccgtcgacga agttgtaagt ggtggatcag atatcatcat tgttggcaga 4560 ggacttttcg ccaagggtag agatcctaag gttgaaggtg aaagatacag aaatgctgga 4620 tgggaagcgt accaaaagag aatcagcgct ccccattaat tatacaggaa acttaataga 4680 acaaatcaca tatttaatct aatagccacc tgcattggca cggtgcaaca ctacttcaac 4740 ttcatcttac aaaaagatca cgtgatctgt tgtattgaac tgaaaatttt ttgtttgctt 4800 ctctctctct ctctttcatt atgtgagatt taaaaaccag aaactacatc atcgaaaaag 4860 tctagagttt tagagctaga aatagcaagt taaaataagg ctagtccgtt atcaacttga 4920 aaaagtggca ccgagtcggt gcttttttta ttttttgtca ctattgcatg ccggtagagg 4980 tgtggtcaat aagagcgacc tcatgctata cctgagaaag caacctgacc tacaggaaag 5040 agttactcaa gaataagaat tttcgtttta aaacctaaga gtcactttaa aatttgtata 5100 cacttatttt ttttataact tatttaataa taaaaatcat aaatcataag aaattcgcct 5160 cgagtcagac ctttctcttc ttttttggag ggtcacctcc tagctgactc aaatcaatgc 5220 gtgtttcata aagaccagtg atggattgat ggataagagt ggcatctaaa acttcttttg 5280 tagacgtata tcgtttacga tcaattgttg tatcaaaata tttaaaagca gcgggagctc 5340 caagattcgt caacgtaaat aaatgaataa tattttctgc ttgttcacgt attggtttgt 5400 ctctatgttt gttatatgca ctaagaactt tatctaaatt ggcatctgct aaaataacac 5460 gcttagaaaa ttcactgatt tgctcaataa tctcatctaa ataatgctta tgctgctcca 5520 caaacaattg tttttgttcg ttatcttctg gactaccctt caacttttca taatgactag 5580 ctaaatataa aaaattcaca tatttgcttg gcagagccag ctcatttcct ttttgtaatt 5640 ctccggcact agccagcatc cgtttacgac cgttttctaa ctcaaaaaga ctatatttag 5700 gtagtttaat gattaagtct tttttaactt ccttatatcc tttagcttct aaaaagtcaa 5760 tcggattttt ttcaaaggaa cttctttcca taattgtgat ccctagtaac tctttaacgg 5820 attttaactt cttcgatttc cctttttcca ccttagcaac cactaggact gaataagcta 5880 ccgttggact atcaaaacca ccatattttt ttggatccca gtctttttta cgagcaataa 5940 gcttgtccga atttcttttt ggtaaaattg actccttgga gaatccgcct gtctgtactt 6000 ctgttttctt gacaatattg acttggggca tggacaatac tttgcgcact gtggcaaaat 6060 ctcgcccttt atcccagaca atttctccag tttccccatt agtttcgatt agagggcgtt 6120 tgcgaatctc tccatttgca agtgtaattt ctgttttgaa gaagttcatg atattagagt 6180 aaaagaaata ttttgcggtt gctttgccta tttcttgctc agacttagca atcattttac 6240 gaacatcata aactttataa tcaccataga caaactccga ttcaagtttt ggatatttct 6300 taatcaaagc agttccaacg acggcattta gatacgcatc atgggcatga tggtaattgt 6360 taatctcacg tactttatag aattggaaat cttttcggaa gtcagaaact aatttagatt 6420 ttaaggtaat cactttaacc tctcgaataa gtttatcatt ttcatcgtat ttagtattca 6480 tgcgactatc caaaatttgt gccacatgct tagtgatttg gcgagtttca accaattggc 6540 gtttgataaa accagcttta tcaagttcac tcaaacctcc acgttcagct ttcgttaaat 6600 tatcaaactt acgttgagtg attaacttgg cgtttagaag ttgtctccaa tagtttttca 6660 tctttttgac tacttcttca cttggaacgt tatccgattt accacgattt ttatcagaac 6720 gcgttaagac cttattgtct attgaatcgt ctttaaggaa actttgtgga acaatgtgat 6780 cgacatcata atcacttaaa cgattaatat ctaattcttg gtccacatac atgtctcttc 6840 cattttggag ataatagaga tagagctttt cattttgcaa ttgagtattt tcaacaggat 6900 gctctttaag aatctgactt cctaattctt tgataccttc ttcgattcgt ttcatacgct 6960 ctcgcgaatt tttctggccc ttttgagttg tctgattttc acgtgccatt tcaataacga 7020 tattttctgg cttatgccgc cccattactt tgaccaattc atcaacaact tttacagtct 7080 gtaaaatacc ttttttaata gcagggctac cagctaaatt tgcaatatgt tcatgtaaac 7140 tatcgccttg tccagacact tgtgcttttt gaatgtcttc tttaaatgtc aaactatcat 7200 catggatcag ctgcataaaa ttgcgattgg caaaaccatc tgatttcaaa aaatctaata 7260 ttgttttgcc agattgctta tccctaatac cattaatcaa ttttcgagac aaacgtcccc 7320 aaccagtata acggcgacgt ttaagctgtt tcatcacctt atcatcaaag aggtgagcat 7380 atgttttaag tctttcctca atcatctccc tatcttcaaa taaggtcaat gttaaaacaa 7440 tatcctctaa gatatcttca ttttcttcat tatccaaaaa atctttatct ttaataattt 7500 ttagcaaatc atggtaggta cctaatgaag cattaaatct atcttcaact cctgaaattt 7560 caacactatc aaaacattct atttttttga aataatcttc ttttaattgc ttaacggtta 7620 cttttcgatt tgttttgaag agtaaatcaa caatggcttt cttctgttca cctgaaagaa 7680 atgctggttt tcgcattcct tcagtaacat atttgacctt tgtcaattcg ttataaaccg 7740 taaaatactc ataaagcaaa ctatgttttg gtagtacttt ttcatttgga agatttttat 7800 caaagtttgt catgcgttca ataaatgatt gagctgaagc acctttatcg acaacttctt 7860 caaaattcca tggggtaatt gtttcttcag acttccgagt catccatgca aaacgactat 7920 tgccacgcgc caatggacca acataataag gaattcgaaa agtcaagatt ttttcaatct 7980 tctcacgatt gtcttttaaa aatggataaa agtcttcttg tcttctcaaa atagcatgca 8040 gctcacccaa gtgaatttga tgggtaatag agccgttgtc aaaggtccgt tgcttgcgca 8100 gcaaatcttc acgatttagt ttcaccaata attcctcagt accatccatt ttttctaaaa 8160 ttggtttgat aaatttataa aattcttctt ggctagctcc cccatcaata taacctgcat 8220 atccgttttt tgattgatca aaaaagattt ctttatactt ttctggaagt tgttgtcgaa 8280 ctaaagcttt taaaagagtc aagtcttgat gatgttcatc gtagcgttta atcattgaag 8340 ctgatagggg agccttagtt atttcagtat ttactcttag gatatctgaa agtaaaatag 8400 catctgataa attcttagct gccaaaaaca aatcagcata ttgatctcca atttgcgcca 8460 ataaattatc taaatcatca tcgtaagtat cttttgaaag ctgtaattta gcatcttctg 8520 ccaaatcaaa atttgattta aaattagggg tcaaacccaa tgacaaagca atgagattcc 8580 caaataagcc atttttcttc tcaccgggga gctgagcaat gagattttct aatcgtcttg 8640 atttactcaa tcgtgcagaa agaatcgctt tagcatctac tccacttgcg ttaatagggt 8700 tttcttcaaa taattgattg taggtttgta ccaactggat aaatagtttg tccacatcac 8760 tattatcagg atttaaatct ccctcaatca aaaaatgacc acgaaactta atcatatgcg 8820 ctaaggccaa atagattaag cgcaaatccg ctttataagt agaatctacc aatttttttc 8880 gcagatgata gatagttgga tatttctcat gataagcaac ttcatctact atatttccaa 8940 aaataggatg acgttcatgc ttcttgtctt cttccaccaa aaaagactct tcaagtcgat 9000 gaaagaaact atcatctact ttcgccatct catttgaaaa aatctcctgt agataacaaa 9060 tacgattctt ccgacgtgta taccttctac gagctgtccg tttgagacga gtcgcttccg 9120 ctgtctctcc actgtcaaat aaaagagccc ctataagatt ttttttgata ctgtggcggt 9180 ctgtatttcc cagaaccttg aactttttag acggaacctt atattcatca gtgatcaccg 9240 cccatccgac gctatttgtg ccgatatcta agcctattga gtatttctta tcgacctttc 9300 tcttcttttt tggaggtttg tcatcgtcat ctttataatc catctagaaa acttagatta 9360 gattgctatg ctttctttct aatgagcaag aagtaaaaaa agttgtaata gaacaagaaa 9420 aatgaaactg aaacttgaga aattgaagac cgtttattaa cttaaatatc aatgggaggt 9480 catcgaaaga gaaaaaaatc aaaaaaaaaa ttttcaagaa aaagaaacgt gataaaaatt 9540 tttattgcct ttttcgacga agaaaaagaa acgaggcggc ctcttttttc ttttccaaac 9600 ctttagtacg ggtaattaac gacaccctag aggaagaaag aggggaaatt tagtatgctg 9660 tgcttgggtg ttttgaagtg gtacggcgat gcgcggagtc cgagaaaatc tggaagagta 9720 aaaaaggagt agaaacattt tgaagatc 9748 <210> 11 <211> 5173 <212> DNA <213> Artificial Sequence <400> 11 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 60 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 120 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 180 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc 240 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat 300 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt 360 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt acccggggat 420 cctctagaga ttgtttaaac tcaggagaac ttccctgtgc atacgggaca gcagagatag 480 gctgttctcg cgcgtttttt tttttttttt gctttttttg gttcctttcg cggatatcgt 540 attatcgata ttacaacaaa aatgtttgga gatctccttc gctgtcttta agtaaaaggc 600 tttaaccgct tatttttctc tctttttttc cttttacccc tcacttgtcg tgagacaatt 660 gtagtatatt tagtaagtat agaaagttct tgttgttaaa aaactcgtta ggatacaata 720 aaaactagaa caaacacaaa agacaaaaaa agacaacaat tgcaggtctc atctggaata 780 taattccccc ctcctgaagc aaatttttcc tttgagccgg aatttttgat attccgagtt 840 ctttttttcc attcgcggag gttattccat tcctaaacga gtggccacaa tgaaacttca 900 attcatatcg accgactatt tttctccgaa ccaaaaaaat agcagggcga gattggagct 960 gcggaaaaaa gaggaaaaaa ttttttcgta gttttcttgt gcaaattagg gtgtaaggtt 1020 tctagggctt attggttcaa gcagaagaga caacaattgt aggtcctaaa ttcaaggcgg 1080 atgtaaggag tattggtttc gaaagttttt ccgaagcggc atggcaggga ctacttgcgc 1140 atgcgctcgg attatcttca tttttgcttg caaaaacgta gaatcatggt aaattacatg 1200 aagaattctc tttttttttt tttttttttt ttttttacct ctaaagagtg ttgaccaact 1260 gaaaaaaccc ttcttcaaga gagttaaact aagactaacc atcataactt ccaaggaatt 1320 aatcgatatc ttgcactcct gatttttctt caaagagaca gcgcaaagga ttatgacact 1380 gttgcattga gtcaaaagtt tttccgaagt gacccagtgc tctttttttt tttccgtgaa 1440 ggactgacaa atatgcgcac aagatccaat acgtaatgga aattcggaaa aactaggaag 1500 aaatgctgca gggcattgcc gtgccgatct tttgtctttc agatatatga gaaaaagaat 1560 attcatcaag tgctgataga agaataccac tcatatgacg tgggcagaag acagcaaacg 1620 taaacatgag ctgctgcgac atttgatggc ttttatccga caagccagga aactccacca 1680 ttatctaatg tagcaaaata tttcttaaca cccgaagttg cgtgtccccc tcacgttttt 1740 aatcatttga attagtatat tgaaattata tataaaggca acaatgtccc cataatcaat 1800 tccatctggg gtctcatgtt ctttccccac cttaaaatct ataaagatat cataatcgtc 1860 aactagttga tatacgtaaa atcatggcgg acacgttatt gattctgggt gatagcctga 1920 gcgccgggta tcgaatgtct gccagcgcgg cctggcctgc cttgttgaat gataagtggc 1980 agagtaaaac gtcggtagtt aatgccagca tcagcggcga cacctcgcaa caaggactgg 2040 cgcgccttcc ggctctgctg aaacagcatc agccgcgttg ggtgctggtt gaactgggcg 2100 gcaatgacgg tttgcgtggt tttcagccac agcaaaccga gcaaacgctg cgccagattt 2160 tgcaggatgt caaagccgcc aacgctgaac cattgttaat gcaaatacgt ctgcctgcaa 2220 actatggtcg ccgttataat gaagccttta gcgccattta ccccaaactc gccaaagagt 2280 ttgatgttcc gctgctgccc ttttttatgg aagaggtcta cctcaagcca caatggatgc 2340 aggatgacgg tattcatccc aaccgcgacg cccagccgtt tattgccgac tggatggcga 2400 agcagttgca gcctttagta aatcatgact cataaaacta acgctaatcg ataaaacatt 2460 agatttcaaa ctagataagg accatgtata agaactatat acttccaata taatatagta 2520 taagctttaa gatagtatct ctcgatctac cgttccacgt gactagtcca aggatttttt 2580 ttaatggatc aacatttcca tgataggaaa gcctcatcat actaaagcac tttttcagtt 2640 ttttgcttta gaactgctac caattataaa atagaatgca caggttatct tgtttggttt 2700 gttattatcc ttttttcttc tttaccagtc atatcacctc attcacgtat gttcgtaatt 2760 tataatgtta aaaatggcat atatagatat atttatgaac atcctaaaaa ataactaaat 2820 ttttctacat ttcaaaaaag gtaacgttga ctgccgcatg gcagtctaat taggagctta 2880 gtgatacata tatatatata tatagtttaa acatcgtcga cctgcaggca tgcaagcttg 2940 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac 3000 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc 3060 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg 3120 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct 3180 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac 3240 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga 3300 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat 3360 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac 3420 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct 3480 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg 3540 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg 3600 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt 3660 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg 3720 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac 3780 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga 3840 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt 3900 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt 3960 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga 4020 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc 4080 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct 4140 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata 4200 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca 4260 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga 4320 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga 4380 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg 4440 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga 4500 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt 4560 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct 4620 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca 4680 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat 4740 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga 4800 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc 4860 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg 4920 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc 4980 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt 5040 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca 5100 cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg 5160 aggccctttc gtc 5173 <210> 12 <211> 11283 <212> DNA <213> Artificial Sequence <400> 12 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 60 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 120 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 180 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc 240 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat 300 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt 360 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt acccggggat 420 cctctagaga ttgtttaaac ctcggccttc gttcatctcg ttctttctac tcttagccct 480 catctttctc ttctgcattc ttttcacctc attaagaaca ttcgcggcgc cgctttaccc 540 caacaaaaac agcccatctt ttgttctacc tctttcagat aaggctcaac agatagacaa 600 taactgatca ttctttcttt tgtttgcaac ccctctgagt tgacataaaa gcgagaatac 660 aaaagaacca gcattagtaa cacatcattt ttttctctgt tcttcactat ttcttgaaaa 720 actaagaagt acgcatcaaa tgcaggtctc atctggaata taattccccc ctcctgaagc 780 aaatttttcc tttgagccgg aatttttgat attccgagtt ctttttttcc attcgcggag 840 gttattccat tcctaaacga gtggccacaa tgaaacttca attcatatcg accgactatt 900 tttctccgaa ccaaaaaaat agcagggcga gattggagct gcggaaaaaa gaggaaaaaa 960 ttttttcgta gttttcttgt gcaaattagg gtgtaaggtt tctagggctt attggttcaa 1020 gcagaagaga caacaattgt aggtcctaaa ttcaaggcgg atgtaaggag tattggtttc 1080 gaaagttttt ccgaagcggc atggcaggga ctacttgcgc atgcgctcgg attatcttca 1140 tttttgcttg caaaaacgta gaatcatggt aaattacatg aagaattctc tttttttttt 1200 tttttttttt ttttttacct ctaaagagtg ttgaccaact gaaaaaaccc ttcttcaaga 1260 gagttaaact aagactaacc atcataactt ccaaggaatt aatcgatatc ttgcactcct 1320 gatttttctt caaagagaca gcgcaaagga ttatgacact gttgcattga gtcaaaagtt 1380 tttccgaagt gacccagtgc tctttttttt tttccgtgaa ggactgacaa atatgcgcac 1440 aagatccaat acgtaatgga aattcggaaa aactaggaag aaatgctgca gggcattgcc 1500 gtgccgatct tttgtctttc agatatatga gaaaaagaat attcatcaag tgctgataga 1560 agaataccac tcatatgacg tgggcagaag acagcaaacg taaacatgag ctgctgcgac 1620 atttgatggc ttttatccga caagccagga aactccacca ttatctaatg tagcaaaata 1680 tttcttaaca cccgaagttg cgtgtccccc tcacgttttt aatcatttga attagtatat 1740 tgaaattata tataaaggca acaatgtccc cataatcaat tccatctggg gtctcatgtt 1800 ctttccccac cttaaaatct ataaagatat cataatcgtc aactagttga tatacgtaaa 1860 atcatgagcg aagaaagctt attcgagtct tctccacaga agatggagta cgaaattaca 1920 aactactcag aaagacatac agaacttcca ggtcatttca ttggcctcaa tacagtagat 1980 aaactagagg agtccccgtt aagggacttt gttaagagtc acggtggtca cacggtcata 2040 tccaagatcc tgatagcaaa taatggtatt gccgccgtga aagaaattag atccgtcaga 2100 aaatgggcat acgagacgtt cggcgatgac agaaccgtcc aattcgtcgc catggccacc 2160 ccagaagatc tggaggccaa cgcagaatat atccgtatgg ccgatcaata cattgaagtg 2220 ccaggtggta ctaataataa caactacgct aacgtagact tgatcgtaga catcgccgaa 2280 agagcagacg tagacgccgt atgggctggc tggggtcacg cctccgagaa tccactattg 2340 cctgaaaaat tgtcccagtc taagaggaaa gtcatcttta ttgggcctcc aggtaacgcc 2400 atgaggtctt taggtgataa aatctcctct accattgtcg ctcaaagtgc taaagtccca 2460 tgtattccat ggtctggtac cggtgttgac accgttcacg tggacgagaa aaccggtctg 2520 gtctctgtcg acgatgacat ctatcaaaag ggttgttgta cctctcctga agatggttta 2580 caaaaggcca agcgtattgg ttttcctgtc atgattaagg catccgaagg tggtggtggt 2640 aaaggtatca gacaagttga acgtgaagaa gatttcatcg ctttatacca ccaggcagcc 2700 aacgaaattc caggctcccc cattttcatc atgaagttgg ccggtagagc gcgtcacttg 2760 gaagttcaac tgctagcaga tcagtacggt acaaatattt ccttgttcgg tagagactgt 2820 tccgttcaga gacgtcatca aaaaattatc gaagaagcac cagttacaat tgccaaggct 2880 gaaacatttc acgagatgga aaaggctgcc gtcagactgg ggaaactagt cggttatgtc 2940 tctgccggta ccgtggagta tctatattct catgatgatg gaaaattcta ctttttagaa 3000 ttgaacccaa gattacaagt cgagcatcca acaacggaaa tggtctccgg tgttaactta 3060 cctgcagctc aattacaaat cgctatgggt atccctatgc atagaataag tgacattaga 3120 actttatatg gtatgaatcc tcattctgcc tcagaaatcg atttcgaatt caaaactcaa 3180 gatgccacca agaaacaaag aagacctatt ccaaagggtc attgtaccgc ttgtcgtatc 3240 acatcagaag atccaaacga tggattcaag ccatcgggtg gtactttgca tgaactaaac 3300 ttccgttctt cctctaatgt ttggggttac ttctccgtgg gtaacaatgg taatattcac 3360 tccttttcgg actctcagtt cggccatatt tttgcttttg gtgaaaatag acaagcttcc 3420 aggaaacaca tggttgttgc cctgaaggaa ttgtccatta ggggtgattt cagaactact 3480 gtggaatact tgatcaaact tttggaaact gaagatttcg aggataacac tattaccacc 3540 ggttggttgg acgatttgat tactcataaa atgaccgctg aaaagcctga tccaactctt 3600 gccgtcattt gcggtgccgc tacaaaggct ttcttagcat ctgaagaagc ccgccacaag 3660 tatatcgaat ccttacaaaa gggacaagtt ctatctaaag acctactgca aactatgttc 3720 cctgtagatt ttatccatga gggtaaaaga tacaagttca ccgtagctaa atccggtaat 3780 gaccgttaca cattatttat caatggttct aaatgtgata tcatactgcg tcaactatct 3840 gatggtggtc ttttgattgc cataggcggt aaatcgcata ccatctattg gaaagaagaa 3900 gttgctgcta caagattatc cgttgactct atgactactt tgttggaagt tgaaaacgat 3960 ccaacccagt tgcgtactcc atcccctggt aaattggtta aattcttggt ggaaaatggt 4020 gaacacatta tcaagggcca accatatgca gaaattgaag ttatgaaaat gcaaatgcct 4080 ttggtttctc aagaaaatgg tatcgtccag ttattaaagc aacctggttc taccattgtt 4140 gcaggtgata tcatggctat tatgactctt gacgatccat ccaaggtcaa gcacgctcta 4200 ccatttgaag gtatgctgcc agattttggt tctccagtta tcgaaggaac caaacctgcc 4260 tataaattca agtcattagt gtctactttg gaaaacattt tgaagggtta tgacaaccaa 4320 gttattatga acgcttcctt gcaacaattg atagaggttt tgagaaatcc aaaactgcct 4380 tactcagaat ggaaactaca catctctgct ttacattcaa gattgcctgc taagctagat 4440 gaacaaatgg aagagttagt tgcacgttct ttgagacgtg gtgctgtttt cccagctaga 4500 caattaagta aattgattga tatggccgtg aagaatcctg aatacaaccc cgacaaattg 4560 ctgggcgccg tcgtggaacc attggcggat attgctcata agtactctaa cgggttagaa 4620 gcccatgaac attctatatt tgtccatttc ttggaagaat attacgaagt tgaaaagtta 4680 ttcaatggtc caaatgttcg tgaggaaaat atcattctga aattgcgtga tgaaaaccct 4740 aaagatctag ataaagttgc gctaactgtt ttgtctcatt cgaaagtttc agcgaagaat 4800 aacctgatcc tagctatctt gaaacattat caaccattgt gcaagttatc ttctaaagtt 4860 tctgccattt tctctactcc tctacaacat attgttgaac tagaatctaa ggctaccgct 4920 aaggtcgctc tacaagcaag agaaattttg attcaaggcg ctttaccttc ggtcaaggaa 4980 agaactgaac aaattgaaca tatcttaaaa tcctctgttg tgaaggttgc ctatggctca 5040 tccaatccaa agcgctctga accagatttg aatatcttga aggacttgat cgattctaat 5100 tacgttgtgt tcgatgtttt acttcaattc ctaacccatc aagacccagt tgtgactgct 5160 gcagctgctc aagtctatat tcgtcgtgct tatcgtgctt acaccatagg agatattaga 5220 gttcacgaag gtgtcacagt tccaattgtt gaatggaaat tccaactacc ttcagctgcg 5280 ttctccacct ttccaactgt taaatctaaa atgggtatga acagggctgt ttctgtttca 5340 gatttgtcat atgttgcaaa cagtcagtca tctccgttaa gagaaggtat tttgatggct 5400 gtggatcatt tagatgatgt tgatgaaatt ttgtcacaaa gtttggaagt tattcctcgt 5460 caccaatctt cttctaacgg acctgctcct gatcgttctg gtagctccgc atcgttgagt 5520 aatgttgcta atgtttgtgt tgcttctaca gaaggtttcg aatctgaaga ggaaattttg 5580 gtaaggttga gagaaatttt ggatttgaat aagcaggaat taatcaatgc ttctatccgt 5640 cgtatcacat ttatgttcgg ttttaaagat gggtcttatc caaagtatta tacttttaac 5700 ggtccaaatt ataacgaaaa tgaaacaatt cgtcacattg agccggcttt ggccttccaa 5760 ctggaattag gaagattgtc caacttcaac attaaaccaa ttttcactga taatagaaac 5820 atccatgtct acgaagctgt tagtaagact tctccattgg ataagagatt ctttacaaga 5880 ggtattatta gaacgggtca tatccgtgat gacatttcta ttcaagaata tctgacttct 5940 gaagctaaca gattgatgag tgatatattg gataatttag aagtcaccga cacttcaaat 6000 tctgatttga atcatatctt catcaacttc attgcggtgt ttgatatctc tccagaagat 6060 gtcgaagccg ccttcggtgg tttcttagaa agatttggta agagattgtt gagattgcgt 6120 gtttcttctg ccgaaattag aatcatcatc aaagatcctc aaacaggtgc cccagtacca 6180 ttgcgtgcct tgatcaataa cgtttctggt tatgttatca aaacagaaat gtacaccgaa 6240 gtcaagaacg caaaaggtga atgggtattt aagtctttgg gtaaacctgg atccatgcat 6300 ttaagaccta ttgctactcc ttaccctgtt aaggaatggt tgcaaccaaa acgttataag 6360 gcacacttga tgggtaccac atatgtctat gacttcccag aattattccg ccaagcatcg 6420 tcatcccaat ggaaaaattt ctctgcagat gttaagttaa cagatgattt ctttatttcc 6480 aacgagttga ttgaagatga aaacggcgaa ttaactgagg tggaaagaga acctggtgcc 6540 aacgctattg gtatggttgc ctttaagatt actgtaaaga ctcctgaata tccaagaggc 6600 cgtcaatttg ttgttgttgc taacgatatc acattcaaga tcggttcctt tggtccacaa 6660 gaagacgaat tcttcaataa ggttactgaa tatgctagaa agcgtggtat cccaagaatt 6720 tacttggctg caaactcagg tgccagaatt ggtatggctg aagagattgt tccactattt 6780 caagttgcat ggaatgatgc tgccaatccg gacaagggct tccaatactt atacttaaca 6840 agtgaaggta tggaaacttt aaagaaattt gacaaagaaa attctgttct cactgaacgt 6900 actgttataa acggtgaaga aagatttgtc atcaagacaa ttattggttc tgaagatggg 6960 ttaggtgtcg aatgtctacg tggatctggt ttaattgctg gtgcaacgtc aagggcttac 7020 cacgatatct tcactatcac cttagtcact tgtagatccg tcggtatcgg tgcttatttg 7080 gttcgtttgg gtcaaagagc tattcaggtc gaaggccagc caattatttt aactggtgct 7140 cctgcaatca acaaaatgct gggtagagaa gtttatactt ctaacttaca attgggtggt 7200 actcaaatca tgtataacaa cggtgtttca catttgactg ctgttgacga tttagctggt 7260 gtagagaaga ttgttgaatg gatgtcttat gttccagcca agcgtaatat gccagttcct 7320 atcttggaaa ctaaagacac atgggataga ccagttgatt tcactccaac taatgatgaa 7380 acttacgatg taagatggat gattgaaggt cgtgagactg aaagtggatt tgaatatggt 7440 ttgtttgata aagggtcttt ctttgaaact ttgtcaggat gggccaaagg tgttgtcgtt 7500 ggtagagccc gtcttggtgg tattccactg ggtgttattg gtgttgaaac aagaactgtc 7560 gagaacttga ttcctgctga tccagctaat ccaaatagtg ctgaaacatt aattcaagaa 7620 cctggtcaag tttggcatcc aaactccgcc ttcaagactg ctcaagctat caatgacttt 7680 aacaacggtg aacaattgcc aatgatgatt ttggccaact ggagaggttt ctctggtggt 7740 caacgtgata tgttcaacga agtcttgaag tatggttcgt ttattgttga cgcattggtg 7800 gattacaaac aaccaattat tatctatatc ccacctaccg gtgaactaag aggtggttca 7860 tgggttgttg tcgatccaac tatcaacgct gaccaaatgg aaatgtatgc cgacgtcaac 7920 gctagagctg gtgttttgga accacaaggt atggttggta tcaagttccg tagagaaaaa 7980 ttgctggaca ccatgaacag attggatgac aagtacagag aattgagatc tcaattatcc 8040 aacaagagtt tggctccaga agtacatcag caaatatcca agcaattagc tgatcgtgag 8100 agagaactat tgccaattta cggacaaatc agtcttcaat ttgctgattt gcacgatagg 8160 tcttcacgta tggtggccaa gggtgttatt tctaaggaac tggaatggac cgaggcacgt 8220 cgtttcttct tctggagatt gagaagaaga ttgaacgaag aatatttgat taaaaggttg 8280 agccatcagg taggcgaagc atcaagatta gaaaagatcg caagaattag atcgtggtac 8340 cctgcttcag tggaccatga agatgatagg caagtcgcaa catggattga agaaaactac 8400 aaaactttgg acgataaact aaagggtttg aaattagagt cattcgctca agacttagct 8460 aaaaagatca gaagcgacca tgacaatgct attgatggat tatctgaagt tatcaagatg 8520 ttatctaccg atgataaaga aaaattgttg aagactttga aataaaacta acgctaatcg 8580 ataaaacatt agatttcaaa ctagataagg accatgtata agaactatat acttccaata 8640 taatatagta taagctttaa gatagtatct ctcgatctac cgttccacgt gactagtcca 8700 aggatttttt ttaaaggaag acatagtttt ttactttccc ccctgccctt cataaacact 8760 acgtttcatt ttctaagagc atcaatttgc aaacacctga actacttgca caatacatag 8820 tttcttctcc tacagataca gttaataatt aataaataac attacacata aacatgttta 8880 agtttcattt cagcaagaca agattgacat tattaagcgt gatctaaact atgaatttct 8940 cccgggtatg aaactacatt ctactattga ttttaataga tgagcgatta aacgggatat 9000 ttgaatttat gactgtttaa acatcgtcga cctgcaggca tgcaagcttg gcgtaatcat 9060 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag 9120 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg 9180 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa 9240 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca 9300 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg 9360 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc 9420 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc 9480 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac 9540 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc 9600 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata 9660 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata 9660 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc 9720 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc 9720 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca 9780 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca 9780 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag 9840 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag 9840 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta 9900 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta 9900 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg 9960 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg 9960 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc 10020 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc 10020 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt 10080 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt 10080 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa 10140 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa 10140 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat 10200 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat 10200 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga 10260 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga 10260 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac 10320 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac 10320 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg 10380 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg 10440 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt 10500 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct 10560 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat 10620 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta 10680 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca 10740 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat 10800 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac 10860 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa 10920 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt 10980 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg 11040 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat 11100 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt 11160 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct 11220 aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc 11280 gtc 11283 <210> 13 <211> 17748 <212> DNA <213> Artificial Sequence <400> 13 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 60 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 120 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 180 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc 240 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat 300 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt 360 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt acccggggat 420 cctctagaga ttgcggccgc gtcaaagcac taatacattg gggctttaag agtgtttata 480 aaggtctaac ctgtaaaaat tatttaaaca acttgaacag gccttaaagt tttcctcatt 540 ccgctcatca tcactaatat tgctctccgt ttttgaatac acacttgaca ctaataagta 600 tcacagaaaa aaagaaaata taataaatta gtattgcgat tacacggtcc aatggataaa 660 cattttttat caacactatg atatataaat ataatagttt ttcgtatata tattcctttt 720 tttggtcaat ttttgaaatt ttcgtagaaa agggagagac aaaacacatt atatcaatga 780 aaacgtacaa aaagtagata aagtcagtgc ttaaacacgt cttttcctta aaaatacttt 840 attattttta ttttattgag agggtggttt aaaaatagaa atagagagag aggtacatac 900 ataaacatac gcgcacaaaa gcagagacta tttcttagta gaaacggcga ccgcgacagc 960 ttggaggtca tcgtgagaaa tagatacctt cacatcggta acaccagctt cttcggcagc 1020 ctttttggcg ttaccgtgca gttcaacggc tggagcgttt ttgttaacgc gtacgatttc 1080 gatgtctttc aatgcagcac caccgcctaa ggacttgacg cctaaggact tgaaaacagc 1140 ctctttggcg gaccatgtcc cagcgaaaga gctttgcaca ctaggctgcg cgctgcagta 1200 ctctatttct tgcggggtga aattgcgctc gataaaagta tcattttcaa cgttgatgct 1260 agtgattaat tcaacatcta caccgacgcc accgttagag actttctcct tggtcatgtt 1320 ttcaatcatc ttggcagttt caatggtgtt agcattgatg taactgtcct tgctttggat 1380 gtttttagag ttgaaagtca aggagcctga tttcttatcc ttagatacac gggctaatgg 1440 gtccaagtaa acatcctctt ccaattcatc agtgtatgga gcatgctctt tacttacgaa 1500 caacttgttg tagatcatac cattatggaa gaatttgtag gcacttttct ctctggcact 1560 aaccttggcg acatactcgt tgtatctgtc ttcagtgata gcaccgtata agtagtctgg 1620 atgaaccaca atagcttgac cacccttttg accaaaaccg aaagaagtga tggacacggc 1680 tctgacaccg tcggtcttta aagtcttgga tgggtacaag acgtattcaa attgctccaa 1740 gatcttatcc acgttatcag cgttacggtt acctggaata ataccactgt ttagaatttg 1800 caaagcacca ttcatcatcc atgcaccagc agcacccttt ggatgaccag tcaagaactt 1860 ttggaaaaca ccaatgacgg gattaccttc agatctaccc aaatgcttca tcatttcatt 1920 aattgtggca gattcgttct tgtcattagc ctttgtggat gtaccgtgga atgaagcgac 1980 acccaagtca tcaatagtta aaccgtaagt agccagtgct cctctcaatg gagcaatacg 2040 tgggtccctc ttgtagaagt cgttacccca ttgttgttgt gcagctctca attgactttc 2100 agcttcgttg tggatttctc tggtacgttc aagtaagaac tcgttttggt cttcgcttgg 2160 aatttcttcg gcctccaact tcaaagcttc caattcgttt tctacccaat ctttaatctg 2220 agcttcacga gtaaccaatt ggcgctttct gtacttcatg ttcaagtttg gtgaagcata 2280 cttaacactg gagtggtgtt cacgagcagt ggttaaaata cccttacctg gagctggcac 2340 agatctacca atcttatcgg tggcggtagc agccatggca acaataccgt aaattggcac 2400 acccatcttc aaagctaaat cagcttgcat gatgatttga ataccagcac cttgagcttc 2460 cataaaaccg ttacgggtag tggtggcagg tctggacatt tccgctgggg tacgaccatg 2520 ttcaaattct tccaaagtgt tggaagtggc cttcatgtta ccgaactcaa aggagccttc 2580 ttcttggaaa tcatcgtaac caccgacaat acagattcta gccttaccag acaagatggt 2640 ttctacacca atgtcaacag attccacgga tgtggcacag gcaccaacag gtgtcttgat 2700 tggaccagat gaggaaatca acaacatatt aacccaagcg gacatggtgt tgataaatga 2760 ttcttgtaaa atatcatttt ggacaggctc atccttgaaa cggtccttaa acataccacg 2820 taaggcagaa acaccaccca taccagaacc agaacagtta ccaacctcag aaacatgtac 2880 gtatttgtac atttcgtatg ggtcggtgat accagatgca ataaatgctt ccacaacaga 2940 gaccaaaacg aataatgtga ttgggtcaac ctgagaaatg atatcatcag agataccata 3000 agtcttagca ttccaaccag ttggaatttg acctgcaacc aaacggtcaa atctcaaagc 3060 ctttggaatg tataaagtgg caccctttag taacttaaca gagtactctc ctgtttctgg 3120 gatttcgaag atatccactt tgtcaccatg ttggtgttta aattgttcgg cagtttcctt 3180 cgaagcctca aatggttcca agtcttcttc gacaatgact tcttgaatca tttccttctt 3240 ttctgggttg taaccattga ataactctgg ttcgatcaat ctgataccac tgtgttctag 3300 gattgatgtt tcatacttgg ccttaacgtc cttgtcatca actggttctt ttgttttgga 3360 atcaacccaa ccagtgtatg gacgaccctt caaattaccg ttatggtatg aaatgaagcc 3420 cataatccag gccatttcaa cgcaaccttc caacgaaaat tcaccaaaag cttccatttc 3480 ccatcttgtt ctggccgaac cccatgggcc gacttcagca aaaccggtaa ctacaataac 3540 tctttccaaa tccaacaaac cttcaagctc agcgggagca atttgtttaa cctgtttgta 3600 tggtttcaat tctgggaagt ccagttgaat gttagctctt ggttgaattt cgacttgagc 3660 atatgcagca tcagcgctat tgccattgac aaccttatgc tccaaagcag tttcgatgga 3720 aactgccttt ctaacttcag aagtttcaac caactcttta cgcaatttag cagtgaattc 3780 cttcaattca ggaacaaatt gcaaaccacc attcaagtca gccataacag gtgatttttg 3840 gcacaattct acgacttctg gagtcaatag acccaataag ttgaaagcca tttccttttg 3900 agagaaagta cgaacaccca tcttttcaat gccttcagca atgatgttat tagcgctcat 3960 taaaccagta cctctagtcc aaccaataat agcaccgcaa acggttaatt gattggccca 4020 ggattcagag tgccatctgt tgaacaaagt ttccaaagac aacttggatt ctgaatacat 4080 accatcacca ccgaaagtac catggtttgg agacattggt agaatgactt gagctggtct 4140 tgtttcaata cctcttgcag atttttgctt cttgacacaa cccatcattc ttaagatatt 4200 ggtcaacatg attctatgag caaattcaga cttagaatca atatgttcta attcaatacc 4260 ttgttctgga atggccgcga atggaataat agcatctaga tcccaaccta aaccaccatt 4320 cttttcagtg tcgtagataa attcaatcaa agcttcaacg tcttgcttag aaccttggtt 4380 gaatggaaca acaatcaaag tagaaccctt agcaccatat ttggcgtaaa tggattggta 4440 gtagtctgta acttgcttag agaaacgaga ggtggtaaca acaaccttag caccaccttg 4500 taacaaacct tgcaagactt cagcaccaat agaaccctta ccagcaccag tgatcaagac 4560 gtatttgtcc ttgaaggtga caccgttgaa ggcagccttt tctaaaccat ctaagaaaag 4620 agaagacaat tggcggtcat atttccaatc tccggcagga gtcttctttc tcaaatgtaa 4680 gaacggaata gtttctcttg gaatagttga agataccgtc ttagaaatgg tagatttgtt 4740 tggcaaagaa gcaacttctt tggtggaatc tttgtccaaa gcatcctcaa catccatacc 4800 ggccaattgg gttgtttgag aaggatcgat ttctttggag ctttccaaga acttcatcaa 4860 atcactatat agtttttcaa attcaaccct ggtggagctg gaaatatctt gtttatcagc 4920 ttgagcactg attgccttgt aaacacgagt caaatcctct tcaatagtag gttgagattc 4980 tttggtgatt ggaccaccaa gggccatttc ttgtacgtat tgagataatt tcctaacctt 5040 ttctcttggc tcttctgagt atgtaatgtt accgttcttg tcaatagcag tttttggacc 5100 ggtaggctta gcaacatctt tgtaaactgg atcaacatcc aaaacttgtt tacagttttc 5160 aatcaactgc tcaccaagag ttttaaccaa ttgatagttt tcaccttttg tttcatcagt 5220 gttagagata tggtattcca tgaatttaat caaagcatcg ttagatctgt tcatgatatt 5280 gatagcttca ctaacaacct ctctatcaac gtttttcaag acaccatgaa ttatctcaaa 5340 gtataatgat aataaagatt gtttagccca gttccaggaa gaatcgaagg ttctggcctt 5400 ttttctagag aaagaagtag caacaccgtt aacaaagaat tcacctaatt cggcattcaa 5460 gtaatccaac tgagcttgaa gttcagcaac agtgtccttt tctttcaaga actttctttc 5520 accgttatcc aagtccattt ttagataacg agccaatact tgcagttgtt gacgcgccaa 5580 aaccttgtgg tctttggtta tttcttccag agcgccagca tcgatcatag ctgcaccggc 5640 agcagcacct gcaccggcag caccactagc gctagcagct gatgataagt caacaccaac 5700 aatggaagcg tatttttgag ccatggagtc caagaaagcc ttggcatcag cttcagaacc 5760 tagacgagca gcaggctcgt tagataaagc taccaaaagg acaccatctt gtctaccaga 5820 tggtagtccc cagcgagttt gtaagtattt tctagcgaca gtaatagtaa acccaccagg 5880 catcttagat gagattaatc ttgataataa cgaggaagat tgcttaccca atgctccaga 5940 gaaggtatct tggaaagttt ctgccaattc ttctaatgga gtttcttctg gtttttcagg 6000 agtagtacca aattctttac ctaaatcacc caaaatttca ttttggactg tagatttacc 6060 accgaccaag tctttgattg tcttggacat tggaatggaa tctaacgact tcttcaactt 6120 gtgagcaacc aaaacgtgca acaataggga agccttgaca ggttcatcgg caatctcagc 6180 agctgctgca gctggggctg ctgccgcgac gggagctggg gctgctgctg caggagcagg 6240 agcactagca gctggagttg gagcaggagc ttcttcctta gcgggctctt cctttgcagc 6300 tagttcggat ggatctgggg tataataaat ctctttggca tccttcgaat agcataagat 6360 ttctctatgt aaagacagag cagcatcgta agattcgtat ttattcttca aggttctttg 6420 agccatccca gccaaagttg gagaaggacc gatttcaaca accctttcag tgttaaaatc 6480 cttcaaaaaa acatcttgag tttcaatcca tctcacagga gaggcaaatt gataagctaa 6540 caattcagtt agcaaaatat gagctaattc ttgctcaact tccggcttca tctcgaggat 6600 tttacgtata tcaactagtt gacgattatg atatctttat agattttaag gtggggaaag 6660 aacatgagac cccagatgga attgattatg gggacattgt tgcctttata tataatttca 6720 atatactaat tcaaatgatt aaaaacgtga gggggacacg caacttcggg tgttaagaaa 6780 tattttgcta cattagataa tggtggagtt tcctggcttg tcggataaaa gccatcaaat 6840 gtcgcagcag ctcatgttta cgtttgctgt cttctgccca cgtcatatga gtggtattct 6900 tctatcagca cttgatgaat attctttttc tcatatatct gaaagacaaa agatcggcac 6960 ggcaatgccc tgcagcattt cttcctagtt tttccgaatt tccattacgt attggatctt 7020 gtgcgcatat ttgtcagtcc ttcacggaaa aaaaaaaaga gcactgggtc acttcggaaa 7080 aacttttgac tcaatgcaac agtgtcataa tcctttgcgc tgtctctttg aagaaaaatc 7140 aggagtgcaa gatatcgatt aattccttgg aagttatgat ggttagtctt agtttaactc 7200 tcttgaagaa gggttttttc agttggtcaa cactctttag aggtaaaaaa aaaaaaaaaa 7260 aaaaaaaaaa gagaattctt catgtaattt accatgattc tacgtttttg caagcaaaaa 7320 tgaagataat ccgagcgcat gcgcaagtag tccctgccat gccgcttcgg aaaaactttc 7380 gaaaccaata ctccttacat ccgccttgaa tttaggacct acaattgttg tctcttctgc 7440 ttgaaccaat aagccctaga aaccttacac cctaatttgc acaagaaaac tacgaaaaaa 7500 ttttttcctc ttttttccgc agctccaatc tcgccctgct atttttttgg ttcggagaaa 7560 aatagtcggt cgatatgaat tgaagtttca ttgtggccac tcgtttagga atggaataac 7620 ctccgcgaat ggaaaaaaag aactcggaat atcaaaaatt ccggctcaaa ggaaaaattt 7680 gcttcaggag gggggaatta tattccagat gagacctgca tgcaggtctc atctggaata 7740 taattccccc ctcctgaagc aaatttttcc tttgagccgg aatttttgat attccgagtt 7800 ctttttttcc attcgcggag gttattccat tcctaaacga gtggccacaa tgaaacttca 7860 attcatatcg accgactatt tttctccgaa ccaaaaaaat agcagggcga gattggagct 7920 gcggaaaaaa gaggaaaaaa ttttttcgta gttttcttgt gcaaattagg gtgtaaggtt 7980 tctagggctt attggttcaa gcagaagaga caacaattgt aggtcctaaa ttcaaggcgg 8040 atgtaaggag tattggtttc gaaagttttt ccgaagcggc atggcaggga ctacttgcgc 8100 atgcgctcgg attatcttca tttttgcttg caaaaacgta gaatcatggt aaattacatg 8160 aagaattctc tttttttttt tttttttttt ttttttacct ctaaagagtg ttgaccaact 8220 gaaaaaaccc ttcttcaaga gagttaaact aagactaacc atcataactt ccaaggaatt 8280 aatcgatatc ttgcactcct gatttttctt caaagagaca gcgcaaagga ttatgacact 8340 gttgcattga gtcaaaagtt tttccgaagt gacccagtgc tctttttttt tttccgtgaa 8400 ggactgacaa atatgcgcac aagatccaat acgtaatgga aattcggaaa aactaggaag 8460 aaatgctgca gggcattgcc gtgccgatct tttgtctttc agatatatga gaaaaagaat 8520 attcatcaag tgctgataga agaataccac tcatatgacg tgggcagaag acagcaaacg 8580 taaacatgag ctgctgcgac atttgatggc ttttatccga caagccagga aactccacca 8640 ttatctaatg tagcaaaata tttcttaaca cccgaagttg cgtgtccccc tcacgttttt 8700 aatcatttga attagtatat tgaaattata tataaaggca acaatgtccc cataatcaat 8760 tccatctggg gtctcatgtt ctttccccac cttaaaatct ataaagatat cataatcgtc 8820 aactagttga tatacgtaaa atccctagga tggacgctta ctccacaaga ccattaaccc 8880 tatctcacgg ttctttagag cacgtgcttc tggtaccaac cgcttcattt ttcattgctt 8940 cgcaattaca agaacaattt aataaaattt tgcccgaacc cactgaaggg tttgctgcag 9000 atgacgagcc taccacacct gctgaactag tggggaaatt ccttggctac gtatcttctc 9060 tagtcgaacc ttccaaggtc ggtcaattcg atcaggtctt gaacctttgc ttaacagaat 9120 ttgaaaactg ttatttagaa ggcaatgaca ttcacgcctt ggctgctaaa ctattacagg 9180 aaaacgacac aactttagtg aagactaaag aactaattaa aaattatatt accgccagaa 9240 taatggctaa gagaccattt gacaaaaaat ccaactctgc tctttttagg gccgtcggcg 9300 agggtaacgc acaattggta gccattttcg gtggtcaagg taacaccgac gactactttg 9360 aagaattgcg tgatctatat caaacttatc atgtcttagt gggagattta atcaagttct 9420 ccgctgaaac tttaagtgaa ctgattagaa ctactttaga tgctgaaaaa gtctttactc 9480 aaggtttaaa catattggaa tggttggaga acccttcaaa taccccagac aaggactatt 9540 tactttccat tccaatttca tgccccttaa ttggtgtcat tcaattggct cactacgtag 9600 ttactgccaa gcttttgggt ttcactccag gtgagttaag atcttactta aaaggtgcta 9660 caggtcactc tcaaggtttg gttactgctg tcgccatagc tgagacggat tcctgggaat 9720 ccttcttcgt ctccgtaaga aaagcaatta ctgtattatt cttcatcggt gttcgttgtt 9780 acgaagcata cccaaacact tccctaccac catccatctt ggaagattcc ttggaaaaca 9840 atgaaggtgt tccatctcca atgttgtcca tttccaatct aactcaagaa caagttcaag 9900 actatgtaaa taagactaac tctcatttgc cagctggtaa acaagttgaa atttctctag 9960 tcaatggtgc gaagaatcta gtcgtatcgg gcccaccaca atcattatat ggtttaaact 10020 tgactttaag aaaggccaag gccccatctg gactggatca atcaagaatc ccattcagcg 10080 aaagaaaatt gaagttctcc aataggttct tacctgttgc atcaccattc cattcccatc 10140 tattggttcc agcttcagat ttgattaaca aagacttagt caaaaacaat gtcagcttta 10200 acgctaaaga tattcaaatc cccgtttacg acacttttga tggttcagat ctaagagtcc 10260 tttcaggttc catttccgag agaatcgtcg actgcatcat tagattacct gtcaaatggg 10320 aaactactac acaattcaaa gccacccaca tattagactt tggtccaggt ggagcttccg 10380 gtttaggtgt tttaacccat cgtaataaag atggtactgg tgttcgtgtt atcgttgccg 10440 gtactctcga cattaaccca gatgatgatt acggattcaa gcaagaaatc tttgatgtta 10500 ctagtaatgg tttgaagaaa aatccaaact ggttggaaga ataccatcca aaattaatta 10560 agaacaaatc aggcaaaatt tttgtcgaaa caaaattttc taaattaatc ggtagaccac 10620 ctttattggt tcctggtatg acaccatgta ctgtttctcc agatttcgta gctgctacca 10680 caaatgctgg ttataccatt gagttggccg gtggtggtta cttttccgca gcaggtatga 10740 ccgccgctat tgattctgtg gtttctcaga tagaaaaggg tagtaccttc ggtatcaact 10800 tgatctacgt caatccattt atgttacaat ggggtattcc attaatcaag gaactaagaa 10860 gcaaaggtta tccaattcaa ttcttgacca ttggtgctgg tgtcccatca ttggaagttg 10920 ctagtgaata catagagaca ttaggtttga agtacttggg tttgaaacca ggttccattg 10980 atgctatttc gcaagttata aacattgcta aagcacatcc aaacttccca atagctttac 11040 aatggaccgg tggtagaggt ggtggtcatc attctttcga agatgcccac actccaatgt 11100 tacaaatgta ctccaagatt agaagacatc caaacattat gttgatattc ggttctggtt 11160 tcggttctgc tgatgacact tacccatact taaccggtga atggtccaca aaattcgatt 11220 atccaccaat gccattcgat ggtttcctat ttggttcgag ggtcatgatt gctaaggaag 11280 ttaaaacttc tcctgatgct aagaagtgta ttgctgcttg tactggtgtt cctgatgata 11340 aatgggaaca aacctacaag aagccaactg gtggtattgt cactgttcgc tctgaaatgg 11400 gtgaaccaat tcacaaaatt gccactcgtg gtgttatgct atggaaggaa ttcgacgaaa 11460 ccatcttcaa cttaccaaag aataagttgg taccaacttt ggaagcaaag agagattaca 11520 ttatctcaag attgaacgcc gatttccaaa aaccatggtt tgctaccgtc aacggtcaag 11580 cccgtgacct agccacaatg acatacgaag aagttgcaaa gagattggtg gaattaatgt 11640 tcatcagatc taccaactct tggtttgatg tcacatggag aacctttact ggtgatttcc 11700 tacgtcgtgt cgaagaacgt ttcactaaaa gtaagacatt gtctttaatc caatcctatt 11760 ctctactaga caagcctgat gaagctattg aaaaagtatt taatgcttat cctgccgcta 11820 gggaacagtt cttgaatgcg caagatattg atcacttttt gagcatgtgt caaaatccaa 11880 tgcaaaaacc agtgcctttt gttccagttt tggatcgtag attcgagatt tttttcaaaa 11940 aagattcgtt atggcaatct gagcacttgg aagccgtcgt cgaccaagac gttcaaagaa 12000 catgtatcct acatggacct gttgcagcac aattcactaa agtcatcgat gaaccaatta 12060 agagcattat ggatggtatt cacgatggtc acatcaaaaa gttactacat caatattacg 12120 gtgacgatga gtcaaagatt ccagcagttg agtactttgg tggtgaaagc cctgtagacg 12180 tacaaagtca agttgattct tcctctgtat ctgaagactc agctgttttt aaggcaacat 12240 cctctactga tgaagaaagc tggtttaagg ctttggcggg atccgaaatt aactggagac 12300 atgcaagttt cttatgttcc tttatcactc aagataaaat gtttgtttct aacccaatta 12360 gaaaagtttt caagccaagc caaggaatgg ttgttgagat ttccaacggc aatacttctt 12420 caaagactgt tgtcactctt tcagaacctg ttcaaggtga attgaaacca actgttattt 12480 tgaagttgtt gaaggagaac ataatccaaa tggaaatgat tgagaacaga actatggatg 12540 gtaagcccgt cagcttgcca ttgttgtaca acttcaaccc agataatggt tttgctccaa 12600 tctctgaagt tatggaggac agaaaccaaa gaattaagga aatgtactgg aaattatgga 12660 ttgatgagcc tttcaatttg gactttgacc caagagatgt cattaagggc aaagatttcg 12720 agatcaccgc taaagaagtt tatgacttta cacacgctgt tggaaacaat tgtgaagact 12780 tcgtttctag acctgataga acgatgttgg ccccaatgga ctttgctatt gttgtcggat 12840 ggagagccat catcaaggcc attttcccta atacggtcga tggtgactta ttgaagttgg 12900 ttcatttgtc taacggctac aagatgattc ctggcgctaa gccactgcaa gttggtgatg 12960 ttgtttcaac tactgctgtt attgaatctg tcgtcaacca acctacagga aagattgtcg 13020 atgtggtagg tacattatcg agaaatggca agcctgtcat ggaagtcacc tcctcattct 13080 tctacagagg caactatact gactttgaaa acactttcca aaagactgtt gaacctgttt 13140 atcaaatgca catcaaaact tctaaagata tagctgtctt gcgctctaag gagtggttcc 13200 aattggacga tgaagacttc gatctgttaa acaaaacttt gactttcgaa actgaaactg 13260 aattggacga tgaagacttc gatctgttaa acaaaacttt gactttcgaa actgaaactg 13260 aagttacttt caagaatgct aacatcttct cttcagtgaa atgttttggc ccaattaaag 13320 aagttacttt caagaatgct aacatcttct cttcagtgaa atgttttggc ccaattaaag 13320 ttgaattgcc aaccaaagaa accgtggaga tcggtattgt cgattacgaa gccggtgcct 13380 ttgaattgcc aaccaaagaa accgtggaga tcggtattgt cgattacgaa gccggtgcct 13380 ctcacggtaa ccctgttgtt gatttcttga agagaaacgg ttccacattg gaacaaaagg 13440 ctcacggtaa ccctgttgtt gatttcttga agagaaacgg ttccacattg gaacaaaagg 13440 tcaatctaga aaatcctatt ccaattgcag tacttgattc gtacactcca agtaccaacg 13500 tcaatctaga aaatcctatt ccaattgcag tacttgattc gtacactcca agtaccaacg 13500 aaccatacgc tagagtttct ggtgatttga atccaattca cgtttcacgt cattttgcct 13560 aaccatacgc tagagtttct ggtgatttga atccaattca cgtttcacgt cattttgcct 13560 cttacgcaaa cttgccaggt actatcacgc acggtatgtt ttcttctgct tccgtccgtg 13620 cttacgcaaa cttgccaggt actatcacgc acggtatgtt ttcttctgct tccgtccgtg 13620 ctttgattga aaactgggct gctgacagtg tttcatccag ggtacgtggc tacacttgtc 13680 ctttgattga aaactgggct gctgacagtg tttcatccag ggtacgtggc tacacttgtc 13680 aatttgttga catggttttg cctaacactg ctttgaaaac atcgattcaa catgttggta 13740 aatttgttga catggttttg cctaacactg ctttgaaaac atcgattcaa catgttggta 13740 tgatcaatgg tagaaaattg ataaagtttg aaactagaaa tgaagatgac gttgtagttt 13800 tgatcaatgg tagaaaattg ataaagtttg aaactagaaa tgaagatgac gttgtagttt 13800 tgactggtga agccgaaatt gaacaacctg ttactacctt cgttttcact ggtcaaggtt 13860 tgactggtga agccgaaatt gaacaacctg ttactacctt cgttttcact ggtcaaggtt 13860 cacaagaaca aggtatgggt atggacttat acaaaacttc taaagctgct caagatgttt 13920 ggaatagagc tgacaaccat ttcaaggaca cttatggttt ctctatctta gacattgtca 13980 ttaacaaccc agttaactta acaattcact tcggtggtga aaagggtaag aggatcagag 14040 aaaactattc tgctatgatc tttgagacta tcgtggatgg aaaattgaag actgaaaaaa 14100 ttttcaagga aattaatgag cacagtactt cttacacatt tagatctgaa aaaggtttat 14160 tgtctgctac tcaatttaca caaccagctt taactttgat ggaaaaagct gctttcgaag 14220 acttgaaatc taaaggtttg atcccagccg atgctacttt tgctggtcac tctttaggtg 14280 agtatgctgc tttggcctct ttggctgatg ttatgtctat cgaatcttta gttgaagttg 14340 tgttctacag aggtatgact atgcaagttg ctgttccaag agatgagttg ggcagatcca 14400 actatggtat gattgccatt aacccaggta gagtcgctgc atcattctct caagaagctt 14460 tgcaatatgt tgttgagaga gttggtaaga gaaccggctg gttggttgaa atcgtcaact 14520 acaacgttga aaaccaacaa tatgttgcag ctggtgatct aagagcttta gacaccgtta 14580 ccaatgttct aaacttcatc aaattacaaa aaattgatat tattgaacta caaaagtcct 14640 ccaatgttct aaacttcatc aaattacaaa aaattgatat tattgaacta caaaagtcct 14640 tatctttgga agaagttgaa ggtcatttgt ttgagatcat tgacgaagct tccaagaaat 14700 tatctttgga agaagttgaa ggtcatttgt ttgagatcat tgacgaagct tccaagaaat 14700 ctgctgtcaa gcctcgccca cttaaattgg agagaggttt tgcttgtatc ccattagttg 14760 ctgctgtcaa gcctcgccca cttaaattgg agagaggttt tgcttgtatc ccattagttg 14760 gtatttctgt tcctttccat tccacctact tgatgaatgg tgttaaacca ttcaagagtt 14820 gtatttctgt tcctttccat tccacctact tgatgaatgg tgttaaacca ttcaagagtt 14820 tcttgaagaa gaatatcata aaagaaaatg tgaaggttgc tagattggcc ggaaagtaca 14880 tcttgaagaa gaatatcata aaagaaaatg tgaaggttgc tagattggcc ggaaagtaca 14880 ttccaaactt gactgcaaaa ccattccagg ttactaagga atatttccag gacgtttatg 14940 ttccaaactt gactgcaaaa ccattccagg ttactaagga atatttccag gacgtttatg 14940 atttgactgg ctccgaacct atcaaggaaa tcatcgacaa ctgggaaaag tatgaacaat 15000 atttgactgg ctccgaacct atcaaggaaa tcatcgacaa ctgggaaaag tatgaacaat 15000 cctaaattga attgaattga aatcgataga tcaatttttt tcttttctct ttccccatcc 15060 cctaaattga attgaattga aatcgataga tcaatttttt tcttttctct ttccccatcc 15060 tttacgctaa aataatagtt tattttattt tttgaatatt ttttatttat atacgtatat 15120 tttacgctaa aataatagtt tattttattt tttgaatatt ttttatttat atacgtatat 15120 atagactatt atttatcttt taatgattat taagattttt attaaaaaaa attacgctcc 15180 atagactatt atttatcttt taatgattat taagattttt attaaaaaaa attacgctcc 15180 tcttttaatg cctttatgca gttttttttc ccattcgata tttctatgtt cgggttcagc 15240 tcttttaatg cctttatgca gttttttttc ccattcgata tttctatgtt cgggttcagc 15240 gtattttaag tttaataact cgaaaattct gcgttcgttg tagaggtttc ctgttttcct 15300 tcgaaccctc tgttttgcga cttttgtttc aattcaacta gtgtcgccaa gttttaacaa 15360 aaagttacaa aatcctagtg agaggccatc ttatgtgcat aacggtactc tctatctatt 15420 tacatatcta atactattca cataactatg acgaatcaat gacatgacta catttaccag 15480 cggccgcatc gtcgacctgc aggcatgcaa gcttggcgta atcatggtca tagctgtttc 15540 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt 15600 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc 15660 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg 15720 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct 15780 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca 15840 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga 15900 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc 15960 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg 16020 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat 16080 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt 16140 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc 16200 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg 16260 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg 16320 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg 16380 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg 16440 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca 16500 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga 16560 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga 16620 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt 16680 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt 16740 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat 16800 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag 16860 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct 16920 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt 16980 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg 17040 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca 17100 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt 17160 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat 17220 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac 17280 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa 17340 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt 17400 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt 17460 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa 17520 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt 17580 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa 17640 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta 17700 tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtc 17748 <210> 14 <211> 9921 <212> DNA <213> Artificial Sequence <400> 14 tgatccaata tcaaaggaaa tgatagcatt gaaggatgag actaatccaa ttgaggagtg 60 gcagcatata gaacagctaa agggtagtgc tgaaggaagc atacgatacc ccgcatggaa 120 tgggataata tcacaggagg tactagacta cctttcatcc tacataaata gacgcatata 180 agtacgcatt taagcataaa cacgcactat gccgttcttc tcatgtatat atatatacag 240 gcaacacgca gatataggtg cgacgtgaac agtgagctgt atgtgcgcag ctcgcgttgc 300 attttcggaa gcgctcgttt tcggaaacgc tttgaagttc ctattccgaa gttcctattc 360 tctagaaagt ataggaactt cagagcgctt ttgaaaacca aaagcgctct gaagacgcac 420 tttcaaaaaa ccaaaaacgc accggactgt aacgagctac taaaatattg cgaataccgc 480 ttccacaaac attgctcaaa agtatctctt tgctatatat ctctgtgcta tatccctata 540 taacctaccc atccaccttt cgctccttga acttgcatct aaactcgacc tctacatttt 600 ttatgtttat ctctagtatt actctttaga caaaaaaatt gtagtaagaa ctattcatag 660 agtgaatcga aaacaatacg aaaatgtaaa catttcctat acgtagtata tagagacaaa 720 atagaagaaa ccgttcataa ttttctgacc aatgaagaat catcaacgct atcactttct 780 gttcacaaag tatgcgcaat ccacatcggt atagaatata atcggggatg cctttatctt 840 gaaaaaatgc acccgcagct tcgctagtaa tcagtaaacg cgggaagtgg agtcaggctt 900 tttttatgga agagaaaata gacaccaaag tagccttctt ctaaccttaa cggacctaca 960 gtgcaaaaag ttatcaagag actgcattat agagcgcaca aaggagaaaa aaagtaatct 1020 aagatgcttt gttagaaaaa tagcgctctc gggatgcatt tttgtagaac aaaaaagaag 1080 tatagattct ttgttggtaa aatagcgctc tcgcgttgca tttctgttct gtaaaaatgc 1140 agctcagatt ctttgtttga aaaattagcg ctctcgcgtt gcatttttgt tttacaaaaa 1200 tgaagcacag attcttcgtt ggtaaaatag cgctttcgcg ttgcatttct gttctgtaaa 1260 aatgcagctc agattctttg tttgaaaaat tagcgctctc gcgttgcatt tttgttctac 1320 aaaatgaagc acagatgctt cgttcaggtg gcacttttcg gggaaatgtg cgcggaaccc 1380 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct 1440 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg 1500 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg 1560 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc 1620 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca 1680 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac 1740 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa 1800 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg 1860 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt 1920 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg 1980 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc 2040 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga 2100 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta 2160 ttgctgataa atctggagcc ggtgagcgtg ggagccgcgg tatcattgca gcactggggc 2220 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg 2280 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt 2340 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa 2400 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt 2460 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt 2520 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt 2580 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga 2640 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag 2700 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata 2760 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg 2820 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga 2880 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca 2940 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa 3000 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt 3060 tgtgatgctc gtcagggggg cggagcctat ggaaagcggc cgcacaatct ttgaaaagat 3120 aatgtatgat tatgctttca ctcatattta tacagaaact tgatgttttc tttcgagtat 3180 atacaaggtg attacatgta cgtttgaagt acaactctag attttgtagt gccctcttgg 3240 gctagcggta aaggtgcgca ttttttcaca ccctacaatg ttctgttcaa aagattttgg 3300 tcaaacgctg tagaagtgaa agttggtgcg catgtttcgg cgttcgaaac ttctccgcag 3360 tgaaagataa atgatccaca tttttagagt catcccgttt tagagctaga aatagcaagt 3420 taaaataagg ctagtccgtt atcaacttga aaaagtggca ccgagtcggt gcaaacaagc 3480 gcaagtggtt tagtggtaaa atccaacgtt gccatcgttg ggcccccggt tcgattccgg 3540 gcttgcgcat ctgaggacag gaggcaaagg ttttagagct agaaatagca agttaaaata 3600 aggctagtcc gttatcaact tgaaaaagtg gcaccgagtc ggtgcaaaca agcgcaagtg 3660 gtttagtggt aaaatccaac gttgccatcg ttgggccccc ggttcgattc cgggcttgcg 3720 cagaatcctg aagaaactgc tggttttaga gctagaaata gcaagttaaa ataaggctag 3780 tccgttatca acttgaaaaa gtggcaccga gtcggtgctt tttttctttt ttgtccacca 3840 ccaaaatgtc aatttttttg ccattttcta aatagttttc ttcactctac aagagatatc 3900 acgattgttt gctttcgata ttggatccat ctaaagtcat ttcaactatc taagatgtgg 3960 ttgtgattgg gccattttgt gaaagccagt acgccagcgt caatacactc ccgtcaatta 4020 gttgcaccat gtccacaaaa tcatatacca gtagagctga gactcatgca agtccggttg 4080 catcgaaact tttacgttta atggatgaaa agaagaccaa tttgtgtgct tctcttgacg 4140 ttcgttcgac tgatgagcta ttgaaacttg ttgaaacgtt gggtccatac atttgccttt 4200 tgaaaacaca cgttgatatc ttggatgatt tcagttatga gggtactgtc gttccattga 4260 aagcattggc agagaaatac aagttcttga tatttgagga cagaaaattc gccgatatcg 4320 gtaacacagt caaattacaa tatacatcgg gcgtttaccg tatcgcagaa tggtctgata 4380 tcaccaacgc ccacggggtt actggtgctg gtattgttgc tggcttgaaa caaggtgcgc 4440 aagaggtcac caaagaacca aggggattat tgatgcttgc tgaattgtct tccaagggtt 4500 ctctagcaca cggtgaatat actaagggta ccgttgatat tgcaaagagt gataaagatt 4560 tcgttattgg gttcattgct cagaacgata tgggaggaag agaagaaggg tttgattggc 4620 taatcatgac cccaggtgta ggtttagacg acaaaggcga tgcattgggt cagcagtaca 4680 gaaccgtcga cgaagttgta agtggtggat cagatatcat cattgttggc agaggacttt 4740 tcgccaaggg tagagatcct aaggttgaag gtgaaagata cagaaatgct ggatgggaag 4800 cgtaccaaaa gagaatcagc gctccccatt aattatacag gaaacttaat agaacaaatc 4860 acatatttaa tctaatagcc acctgcattg gcacggtgca acactacttc aacttcatct 4920 tacaaaaaga tcacgtgatc tgttgtattg aactgaaaat tttttgtttg cttctctctc 4980 tctctctttc attatgtgag atttaaaaac cagaaactac atcatcgaaa aagtctagag 5040 ttttagagct agaaatagca agttaaaata aggctagtcc gttatcaact tgaaaaagtg 5100 gcaccgagtc ggtgcttttt ttattttttg tcactattgc atgccggtag aggtgtggtc 5160 aataagagcg acctcatgct atacctgaga aagcaacctg acctacagga aagagttact 5220 caagaataag aattttcgtt ttaaaaccta agagtcactt taaaatttgt atacacttat 5280 tttttttata acttatttaa taataaaaat cataaatcat aagaaattcg cctcgagtca 5340 gacctttctc ttcttttttg gagggtcacc tcctagctga ctcaaatcaa tgcgtgtttc 5400 ataaagacca gtgatggatt gatggataag agtggcatct aaaacttctt ttgtagacgt 5460 atatcgttta cgatcaattg ttgtatcaaa atatttaaaa gcagcgggag ctccaagatt 5520 cgtcaacgta aataaatgaa taatattttc tgcttgttca cgtattggtt tgtctctatg 5580 tttgttatat gcactaagaa ctttatctaa attggcatct gctaaaataa cacgcttaga 5640 aaattcactg atttgctcaa taatctcatc taaataatgc ttatgctgct ccacaaacaa 5700 ttgtttttgt tcgttatctt ctggactacc cttcaacttt tcataatgac tagctaaata 5760 taaaaaattc acatatttgc ttggcagagc cagctcattt cctttttgta attctccggc 5820 actagccagc atccgtttac gaccgttttc taactcaaaa agactatatt taggtagttt 5880 aatgattaag tcttttttaa cttccttata tcctttagct tctaaaaagt caatcggatt 5940 tttttcaaag gaacttcttt ccataattgt gatccctagt aactctttaa cggattttaa 6000 cttcttcgat ttcccttttt ccaccttagc aaccactagg actgaataag ctaccgttgg 6060 actatcaaaa ccaccatatt tttttggatc ccagtctttt ttacgagcaa taagcttgtc 6120 cgaatttctt tttggtaaaa ttgactcctt ggagaatccg cctgtctgta cttctgtttt 6180 cttgacaata ttgacttggg gcatggacaa tactttgcgc actgtggcaa aatctcgccc 6240 tttatcccag acaatttctc cagtttcccc attagtttcg attagagggc gtttgcgaat 6300 ctctccattt gcaagtgtaa tttctgtttt gaagaagttc atgatattag agtaaaagaa 6360 atattttgcg gttgctttgc ctatttcttg ctcagactta gcaatcattt tacgaacatc 6420 ataaacttta taatcaccat agacaaactc cgattcaagt tttggatatt tcttaatcaa 6480 agcagttcca acgacggcat ttagatacgc atcatgggca tgatggtaat tgttaatctc 6540 acgtacttta tagaattgga aatcttttcg gaagtcagaa actaatttag attttaaggt 6600 aatcacttta acctctcgaa taagtttatc attttcatcg tatttagtat tcatgcgact 6660 atccaaaatt tgtgccacat gcttagtgat ttggcgagtt tcaaccaatt ggcgtttgat 6720 aaaaccagct ttatcaagtt cactcaaacc tccacgttca gctttcgtta aattatcaaa 6780 cttacgttga gtgattaact tggcgtttag aagttgtctc caatagtttt tcatcttttt 6840 gactacttct tcacttggaa cgttatccga tttaccacga tttttatcag aacgcgttaa 6900 gaccttattg tctattgaat cgtctttaag gaaactttgt ggaacaatgt gatcgacatc 6960 ataatcactt aaacgattaa tatctaattc ttggtccaca tacatgtctc ttccattttg 7020 gagataatag agatagagct tttcattttg caattgagta ttttcaacag gatgctcttt 7080 aagaatctga cttcctaatt ctttgatacc ttcttcgatt cgtttcatac gctctcgcga 7140 atttttctgg cccttttgag ttgtctgatt ttcacgtgcc atttcaataa cgatattttc 7200 tggcttatgc cgccccatta ctttgaccaa ttcatcaaca acttttacag tctgtaaaat 7260 acctttttta atagcagggc taccagctaa atttgcaata tgttcatgta aactatcgcc 7320 ttgtccagac acttgtgctt tttgaatgtc ttctttaaat gtcaaactat catcatggat 7380 cagctgcata aaattgcgat tggcaaaacc atctgatttc aaaaaatcta atattgtttt 7440 gccagattgc ttatccctaa taccattaat caattttcga gacaaacgtc cccaaccagt 7500 ataacggcga cgtttaagct gtttcatcac cttatcatca aagaggtgag catatgtttt 7560 aagtctttcc tcaatcatct ccctatcttc aaataaggtc aatgttaaaa caatatcctc 7620 taagatatct tcattttctt cattatccaa aaaatcttta tctttaataa tttttagcaa 7680 atcatggtag gtacctaatg aagcattaaa tctatcttca actcctgaaa tttcaacact 7740 atcaaaacat tctatttttt tgaaataatc ttcttttaat tgcttaacgg ttacttttcg 7800 atttgttttg aagagtaaat caacaatggc tttcttctgt tcacctgaaa gaaatgctgg 7860 ttttcgcatt ccttcagtaa catatttgac ctttgtcaat tcgttataaa ccgtaaaata 7920 ctcataaagc aaactatgtt ttggtagtac tttttcattt ggaagatttt tatcaaagtt 7980 tgtcatgcgt tcaataaatg attgagctga agcaccttta tcgacaactt cttcaaaatt 8040 ccatggggta attgtttctt cagacttccg agtcatccat gcaaaacgac tattgccacg 8100 cgccaatgga ccaacataat aaggaattcg aaaagtcaag attttttcaa tcttctcacg 8160 attgtctttt aaaaatggat aaaagtcttc ttgtcttctc aaaatagcat gcagctcacc 8220 caagtgaatt tgatgggtaa tagagccgtt gtcaaaggtc cgttgcttgc gcagcaaatc 8280 ttcacgattt agtttcacca ataattcctc agtaccatcc attttttcta aaattggttt 8340 gataaattta taaaattctt cttggctagc tcccccatca atataacctg catatccgtt 8400 ttttgattga tcaaaaaaga tttctttata cttttctgga agttgttgtc gaactaaagc 8460 ttttaaaaga gtcaagtctt gatgatgttc atcgtagcgt ttaatcattg aagctgatag 8520 gggagcctta gttatttcag tatttactct taggatatct gaaagtaaaa tagcatctga 8580 taaattctta gctgccaaaa acaaatcagc atattgatct ccaatttgcg ccaataaatt 8640 atctaaatca tcatcgtaag tatcttttga aagctgtaat ttagcatctt ctgccaaatc 8700 aaaatttgat ttaaaattag gggtcaaacc caatgacaaa gcaatgagat tcccaaataa 8760 gccatttttc ttctcaccgg ggagctgagc aatgagattt tctaatcgtc ttgatttact 8820 caatcgtgca gaaagaatcg ctttagcatc tactccactt gcgttaatag ggttttcttc 8880 aaataattga ttgtaggttt gtaccaactg gataaatagt ttgtccacat cactattatc 8940 aggatttaaa tctccctcaa tcaaaaaatg accacgaaac ttaatcatat gcgctaaggc 9000 caaatagatt aagcgcaaat ccgctttata agtagaatct accaattttt ttcgcagatg 9060 atagatagtt ggatatttct catgataagc aacttcatct actatatttc caaaaatagg 9120 atgacgttca tgcttcttgt cttcttccac caaaaaagac tcttcaagtc gatgaaagaa 9180 actatcatct actttcgcca tctcatttga aaaaatctcc tgtagataac aaatacgatt 9240 cttccgacgt gtataccttc tacgagctgt ccgtttgaga cgagtcgctt ccgctgtctc 9300 tccactgtca aataaaagag cccctataag attttttttg atactgtggc ggtctgtatt 9360 tcccagaacc ttgaactttt tagacggaac cttatattca tcagtgatca ccgcccatcc 9420 gacgctattt gtgccgatat ctaagcctat tgagtatttc ttatcgacct ttctcttctt 9480 ttttggaggt ttgtcatcgt catctttata atccatctag aaaacttaga ttagattgct 9540 atgctttctt tctaatgagc aagaagtaaa aaaagttgta atagaacaag aaaaatgaaa 9600 ctgaaacttg agaaattgaa gaccgtttat taacttaaat atcaatggga ggtcatcgaa 9660 agagaaaaaa atcaaaaaaa aaattttcaa gaaaaagaaa cgtgataaaa atttttattg 9720 cctttttcga cgaagaaaaa gaaacgaggc ggcctctttt ttcttttcca aacctttagt 9780 acgggtaatt aacgacaccc tagaggaaga aagaggggaa atttagtatg ctgtgcttgg 9840 gtgttttgaa gtggtacggc gatgcgcgga gtccgagaaa atctggaaga gtaaaaaagg 9900 agtagaaaca ttttgaagat c 9921 <210> 15 <211> 9377 <212> DNA <213> Artificial Sequence <400> 15 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 60 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 120 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 180 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc 240 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat 300 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt 360 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt acccggggat 420 cctctagaga ttgtttaaac tttcctctaa tcaggttcca ccaaacagat accccggtgt 480 ttcacggaat ggtacgtttg atatcgctga tttgagagga ggttacactt gaagaatcac 540 agtcttgcga ccggctattc aacaaggcat tcccccaagt ttgaattctt tgaaatagat 600 tgctattagc tagtaatcca ccaaatcctt cgctgctcac caatggaatc gcaagatgcc 660 cacgatgaga ctgttcaggt taaacgcaaa agaaacacac tctgggaatt tcttcccaaa 720 ttgtatctct caatacgcat caacccatgt caattaaaca cgctgtatag agactaggca 780 gatctgacga tcacctagcg actctctcca ccgtttgacg aggccattta caaaaacata 840 acgaacgaca agcctactcg aattcgtttc caaactcttt tcgaacttgt cttcaactgc 900 tttcgcatga agtacctccc aactactttt cctcacactt gtactccatg actaaacccc 960 ccctcccatt acaaactaaa atcttacttt tattttcttt tgccctctct gtcgctctgc 1020 cttaactacg tatttctcgc cgagaaaaac ttcaatttaa gctattctcc aaaaatctta 1080 gcgtatattt tttttccaaa gtgagcaaat taaagccttc gagcgtccca aaaccttctc 1140 aagcaaggtt ttcagtataa tgttacatgc gtacacgcgt ttgtacagaa aaaaaagaaa 1200 aatttgaaat ataaataacg ttcttaatac taacataact ataaaaaaat aaatagggac 1260 ctagacttca ggttgtctaa ctccttcctt ttcggttaga gcggatgtgg ggggagggcg 1320 tgaatgtaag cgtgacataa ctaattacat gatatcgaca aaggaaaagg ggcctgttta 1380 tattgccagc gtcgatgttg atgacaacgc agagttcata cggttttggt tttgggcttc 1440 acttgtctca gtaggttgag ggtttgtatg aagtttcaga ggctccgtta gtaaatgacc 1500 cattctttct atcttggtcc tgagataacc ttctatctcc ttggagtcta tgccttcact 1560 ggagtttgtc cagtgtatgg gtaccatagg cactcgttca acgcacttaa ggtaaggcgc 1620 atgatctacc tgttttatct tttccgggtt atttgtcagc agcctgacat taccgatgcc 1680 aagatccagc aaaatagcct tacccagcga gaagtcccta gcatccacgg gatgtttcag 1740 cattaagttg gcctgcactg tgtcagcacc taagtcttgc aggttgtagg ccttgagttt 1800 ctcacctaac ccgatgccac gaccctcttg tcttagatac acgataacac catggccgtt 1860 tccacctttg atgttgcttg tgggttcgtg gtcgcaagcg ataagcctac cggccctatc 1920 gaattgttca ccacaatcac aacgggcgct ccatgcgttt tcaccggtgt aacattcaga 1980 atggatccgt accagcgtgt cgttatgggc gtcccatgta gtggctttgg aagctaataa 2040 ctcacctgta ctatcatcaa actccagagc taatccgagt ctatcgtctt cgtctgccac 2100 agttctgccg ggatacagtt tgccaatata agcgcccctg atcattctat cttgttgcgt 2160 ctcgcactgt cttctacgga atagcgagcg cgaccgtatg tcttcaccaa acacaatggc 2220 tagatgttcc ttgttgtccc tgttgttact gtaaagatgt aaaaagatat ccggaccctg 2280 tgtggttggg atacgagctc ttgcgacaca ttgtactaga ggcaagccgc catcgccgtt 2340 cctgccatca cccgtaccac taacctcgta tttgctgcta tcctgtttac tgttgtcgta 2400 gttatctatg gtcattatag ttttttctcc ttgacgttaa agtatagagg tatattaaca 2460 attttttgtt gatactttta tgacatttga ataagaagta atacaaaccg aaaatgttga 2520 aagtattagt taaagtggtt atgcagcttt tgcatttata tatctgttaa tagatcaaaa 2580 atcatcgctt cgctgattaa ttaccccaga aataaggcta aaaaactaat cgcattatta 2640 tcctatggtt gttaatttga ttcgttgatt tgaaggtttg tggggccagg ttactgccaa 2700 tttttcctct tcataaccat aaaagctagt attgtagaat ctttattgtt cggagcagtg 2760 cggcgcgagg cacatctgcg tttcaggaac gcgaccggtg aagaccagga cgcacggagg 2820 agagtcttcc gtcggagggc tgtcgcccgc tcggcggctt ctaatccgta cttcaatata 2880 gcaatgagca gttaagcgta ttactgaaag ttccaaagag aaggtttttt taggctaaga 2940 taatggggct ctttacattt ccacaacata taagtaagat tagatatgga tatgtatatg 3000 gtggtattgc catgtaatat gattattaaa cttctttgcg tccatccaaa aaaaaagtaa 3060 gaatttttga aaattcaata taaatgtctt tgacaccact gtgtgaagat ttaccacaat 3120 ttctgcaaaa ctatctaccg aatgctggtc aaacggaaaa taccattgtg ccctttgtca 3180 cactaactta tgctcaatcg ctcgacgcga gagtatctag gggccctgga gtgaggacta 3240 caatttcaca tcccgagacc aaaacaatga cgcattattt gagacatcat cacgatggaa 3300 tactcgtagg aagtggaaca gtgctagctg ataatcctgg attgaattgt aaatggggtc 3360 ccgatccggc tgcaaattcc ccaaggccaa taataataga tacaaagcaa aagtggcgat 3420 ttgatggttc aaaaatgcaa gaacttttta ttaaacgaca gggtaagccg ccaatcgttg 3480 ttgtcacaag tgagcccatt ataaaagaac aacatgtaga ctacgcaatt tgtccaataa 3540 atgatactac gaaattggtc gattggaaga aattgtttga gatattaaaa gaagaattca 3600 atataaggtc agtaatggtt gaaggaggtg ccaatgtaat aaatcagttg ttgctgagga 3660 gcgatattgt caacagtctt ataataacta ttggatcaac atttctgggt agctcaggca 3720 ccgaagttag cccaccccaa acagtaaatt taaaggatat gtcatggtgg aagggcatta 3780 ccgatgtggt gctttgtgcg agactggccg atgactaatt tgccagctta ctatccttct 3840 tgaaaatatg cactctatat cttttagttc ttaattgcaa cacatagatt tgctgtataa 3900 cgaattttat gctatttttt aaatttggag ttcagtgata aaagtgtcac agcgaatttc 3960 ctcacatgta gggaccgaat tgtttacaag ttctctgtac caccatggag acatcaaaga 4020 ttgaaaatct atggaaagat atggacggta gcaacaagaa tatagcacga gccgcgaagt 4080 tcatttcgtt acttttgata tcgctcacaa ctattgcgaa gcgcttcagt gaaaaaatca 4140 taaggaaaag ttgtaaatat tattggtagt attcgtttgg taaagtagag ggggtaattt 4200 ttccccttta ttttgttcat acattcttaa attgctttgc ctctcctttt ggaaagctat 4260 acttcggagc actgttgagc gaaggctcat tagatatatt ttctgtcatt ttccttaacc 4320 caaaaataag ggaaagggtc caaaaagcgc tcggacaact gttgaccgtg atccgaagga 4380 ctggctatac agtgttcaca aaatagccaa gctgaaaata atgtgtagct atgttcagtt 4440 agtttggcta gcaaagatat aaaagcaggt cggaaatatt tatgggcatt attatgcaga 4500 gcatcaacat gataaaaaaa aacagttgaa tattccctca aaaatggagg actctaataa 4560 tgaagccagc gatgacttca acaacttatt aaacaaagaa attgagtctg caaaggaagt 4620 gaagttaaga aaattcgcga acaggaataa taataggaac gaaaacagtt ccaaagtcaa 4680 agatgcgagt ggattcagac tgagagttat tcaaaccgat gggcacaaaa ctaaaaagac 4740 agaccctgat tatgaagtga caattgatgg acccttaagg aagattgagc cttatttttt 4800 cacttataaa actttctgta aggagaggtg gagagatcgt aagttggtgg atgtattcgt 4860 gagcgaattc agggatcgag agcccagcta ttattccaaa accattgcag agggaaaagt 4920 atacttaaac gatgaacctg caaaccttga taccatcatt cgtgacggtg atctgatcac 4980 acataaagta catcgacatg aaccgccagt cacatctaaa ccaatagata ttgtgtttga 5040 agacgaagat atccttgtca ttgataaacc cagcagtatt ccagtgcacc caacgggcag 5100 atacagattc aataccatta caaaaatgct agaaagacag ctaggttatt cagtacatcc 5160 atgtaataga ctagacaagc caactagtgg actaatgttt ttagccaaga ctccattagg 5220 tgcagataga atgggagacc aaatgaaagc gagggaagtc accaaagaat acgtagcccg 5280 cgtgaagggc gagttcccta taggtatagt agaggtagat aagcctgtca gatctgttaa 5340 ccctaaagtt gcactaaacg ctgtttgcga gatgagcgat gaaaacgcta aacatgccaa 5400 gaccgttttc caaagggtta gctatgatgg acagacgagt attgtaaagt gtaaaccatt 5460 gacaggtaga actcaccaaa taagagtcca tttgcagtac ttgggatttc ccatagcaaa 5520 tgaccctata tactcgaacc cagatatctg gggtccagat ttgggccgcg gtggacttca 5580 aaactatgat gacatcgttc tgaaactaga cgctattggc aagactaatc ctgcagagag 5640 ctggattcat cctcatagcg agggtgaata cttgcttggc cgtcagtgcg aagaatgcga 5700 ggctgaaatg tacacagatc ccggtactaa tgatctcgac ctctggctgc atgccttccg 5760 gtacgagtca ttggaaagaa attcggatac gcaaaaacct ctctggagtt atagaacaaa 5820 ataccctgaa tgggccttag agccacatcg ccgatatatg gaaatggccg ttaaggaagc 5880 tggtaagtgt ggcccgacaa agactgcttt tagtgttggt gccgttcttg ttcatggaac 5940 tcaagtactt gccacaggat attcaagaga gctaccagga aacactcatg cagaacagtg 6000 tgctttgata aagtactcgc agttacaccc gaactgtccc actatagtcc ctatgggaac 6060 agtgctctat acaaccatgg aaccctgttc cttcaggcta agtggcaatg agccttgctg 6120 cgatagaatc ctggccaccc aaggtgccat tggtactgtc ttcgtggggg ttatggagcc 6180 cgatacattt gttaaaaaca atacaagttt gaacaagctg gaatcgcacg gtgtgaacta 6240 catacaaata ccaggctacg aggaggagtg caccatcatt gccttcaaag gccacgataa 6300 tagtgacgac aaagcttagg cgaatttctt atgatttatg atttttatta ttaaataagt 6360 tataaaaaaa ataagtgtat acaaatttta aagtgactct taggttttaa aacgaaaatt 6420 cttattcttg agtaactctt tcctgtaggt caggttgctt tctcaggtat agcatgaggt 6480 cgctcttatt gaccacacct ctaccggcag gtgccccggg taacccagtt cctcactatt 6540 ttttactgcg gaagcggaag cggaaaatac ggaaacgcgc gggaacatac aaaacataca 6600 aaatatacct ttctcacaca agaaatatat gctacttgca aaatatcata ccaaaaaact 6660 tttcacaacc gaaaccaaaa ccaacggata tcatacatta cactaccacc attcaaactt 6720 tactactatc ctcccttcag tttccctttt tctgcctttt tcggtgacgg aaatacgctt 6780 cagagaccct aaagggaaat ccatgccata acaggaaagt aacatcccaa tgcggactat 6840 accaccccac cacactccta ccaataacgg taactattct atgttttctt actcctatgt 6900 ctattcatct ttcatctgac tacctaatac tatgcaaaaa tgtaaaatca tcacacaaaa 6960 cataaacaat caaaatcagc catttccgca ccttttcctc tgtccacttt caaccgtccc 7020 tccaaatgta aaatggccta tcggaataca ttttctacat cctaactact ataaaacaac 7080 ctttagactt acgtttgcta ctctcatggt ttaaacatcg tcgacctgca ggcatgcaag 7140 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc 7200 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta 7260 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca 7320 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc 7380 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc 7440 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat 7500 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt 7560 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg 7620 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc 7680 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt 7740 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa 7800 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta 7860 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa 7920 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa 7980 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt 8040 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt 8100 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat 8160 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat 8220 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc 8280 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc 8340 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta 8400 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga 8460 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg 8520 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc 8580 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat 8640 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag 8700 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat 8760 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa 8820 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa 8880 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga 8940 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg 9000 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc 9060 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg 9120 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact 9180 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat 9240 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt 9300 gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat 9360 cacgaggccc tttcgtc 9377 <210> 16 <211> 7682 <212> DNA <213> Artificial Sequence <400> 16 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 60 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 120 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 180 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc 240 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat 300 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt 360 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt acccggggat 420 cctctagaga ttgtttaaac tttcctctaa tcaggttcca ccaaacagat accccggtgt 480 ttcacggaat ggtacgtttg atatcgctga tttgagagga ggttacactt gaagaatcac 540 agtcttgcga ccggctattc aacaaggcat tcccccaagt ttgaattctt tgaaatagat 600 tgctattagc tagtaatcca ccaaatcctt cgctgctcac caatggaatc gcaagatgcc 660 cacgatgaga ctgttcaggt taaacgcaaa agaaacacac tctgggaatt tcttcccaaa 720 ttgtatctct caatacgcat caacccatgt caattaaaca cgctgtatag agactaggca 780 gatctgacga tcacctagcg actctctcca ccgtttgacg aggccattta caaaaacata 840 acgaacgaca agcctactcg aattcgtttc caaactcttt tcgaacttgt cttcaactgc 900 tttcgcatga agtacctccc aactactttt cctcacactt gtactccatg actaaacccc 960 ccctcccatt acaaactaaa atcttacttt tattttcttt tgccctctct gtcgctctgc 1020 cttaactacg tatttctcgc cgagaaaaac ttcaatttaa gctattctcc aaaaatctta 1080 gcgtatattt tttttccaaa gtgagcaaat taaagccttc gagcgtccca aaaccttctc 1140 aagcaaggtt ttcagtataa tgttacatgc gtacacgcgt ttgtacagaa aaaaaagaaa 1200 aatttgaaat ataaataacg ttcttaatac taacataact ataaaaaaat aaatagggac 1260 ctagacttca ggttgtctaa ctccttcctt ttcggttaga gcggatgtgg ggggagggcg 1320 tgaatgtaag cgtgacataa ctaattacat gatatcgaca aaggaaaagg ggcctgttta 1380 tttcttcaaa tattgggcca attcttcgat ggagatcaaa ggaatgccat gtttcttacc 1440 aaacgcttgg cagtcattta atctcatcat agttccttgt tcgtcatcgt taaccaattc 1500 gccaataaca gcgacgggac ttagtccact tagtttacac aaatcgacac cggcctcagt 1560 gtggcctctt ctctgcaaaa caccgccatc agcggctctc aagggacaga tgtgccctgg 1620 ttttaaaaat gattttggcg tagaggaaga gtctgcaaga gccctacaag tcatcgacct 1680 gtcgtgagca gaaatacctg tggtagtgcc ttgggctaca tctacagtta ttgtgtatgc 1740 agttccatgc ctgtcgtcat cattggattc aaacttcatg cctgttctca gtagtggaag 1800 gtccaactta tcagcaatgg cattcgtcat aggtgcacag acgtagcctg aggaatggcg 1860 tacaagaaag gccatttgtt cagtgctgac attttcagca gcgcaaataa gatcaccttc 1920 attttcacga ccggcatcgt ccataacaat tacaaactta ttctgcttga agtgttctat 1980 agcttgatca attggtgtaa acattatagt tttttctcct tgacgttaaa gtatagaggt 2040 atattaacaa ttttttgttg atacttttat gacatttgaa taagaagtaa tacaaaccga 2100 aaatgttgaa agtattagtt aaagtggtta tgcagctttt gcatttatat atctgttaat 2160 agatcaaaaa tcatcgcttc gctgattaat taccccagaa ataaggctaa aaaactaatc 2220 gcattattat cctatggttg ttaatttgat tcgttgattt gaaggtttgt ggggccaggt 2280 tactgccaat ttttcctctt cataaccata aaagctagta ttgtagaatc tttattgttc 2340 ggagcagtgc ggcgcgaggc acatctgcgt ttcaggaacg cgaccggtga agaccaggac 2400 gcacggagga gagtcttccg tcggagggct gtcgcccgct cggcggcttc taatccgtac 2460 ttcaatatag caatgagcag ttaagcgtat tactgaaagt tccaaagaga aggttttttt 2520 aggctaagat aatggggctc tttacatttc cacaacatat aagtaagatt agatatggat 2580 atgtatatgg tggtattgcc atgtaatatg attattaaac ttctttgcgt ccatccaaaa 2640 aaaaagtaag aatttttgaa aattcaatat aaatggcagt taaaggatta ggcaaaccag 2700 atcaagttta tgacggttcc aaaatcaggg tcggtatcat tcatgcccgt tggaaccgtg 2760 tcattattga cgctcttgtg aaaggtgcta ttgaaagaat ggcttccctt ggtgtcgagg 2820 aaaataacat aataattgaa actgttcctg gatcctatga attaccttgg gggacaaaaa 2880 ggtttgttga cagacaagcg aagttgggaa agccgctaga tgttgtaatt cctattggtg 2940 tacttatcaa aggtagtaca atgcactttg aatacatttc agattccacc actcacgcat 3000 tgatgaactt acaagaaaaa gtcgacatgc ccgtcatttt tggcctctta acttgcatga 3060 ctgaagaaca agccctggcc agagctggca tcgatgaagc gcactccatg cacaaccatg 3120 gtgaagattg gggtgctgca gcagtggaaa tggctgttaa gttcggtaaa aatgcttttt 3180 gatttgccag cttactatcc ttcttgaaaa tatgcactct atatctttta gttcttaatt 3240 gcaacacata gatttgctgt ataacgaatt ttatgctatt ttttaaattt ggagttcagt 3300 gataaaagtg tcacagcgaa tttcctcaca tgtagggacc gaattgttta caagttctct 3360 gtaccaccat ggagacatca aagattgaaa atctatggaa agatatggac ggtagcaaca 3420 agaatatagc acgagccgcg aagttcattt cgttactttt gatatcgctc acaactattg 3480 cgaagcgctt cagtgaaaaa atcataagga aaagttgtaa atattattgg tagtattcgt 3540 ttggtaaagt agagggggta atttttcccc tttattttgt tcatacattc ttaaattgct 3600 ttgcctctcc ttttggaaag ctatacttcg gagcactgtt gagcgaaggc tcattagata 3660 tattttctgt cattttcctt aacccaaaaa taagggaaag ggtccaaaaa gcgctcggac 3720 aactgttgac cgtgatccga aggactggct atacagtgtt cacaaaatag ccaagctgaa 3780 aataatgtgt agctatgttc agttagtttg gctagcaaag atataaaagc aggtcggaaa 3840 tatttatggg cattattatg cagagcatca acatgataaa aaaaaacagt tgaatattcc 3900 ctcaaaaatg tttactggta ttgtagaatg catggggact gttttggaaa acaacccata 3960 tgatgactct gaaagtggag gtcaaggagt ttctattact attggcaatg cggggagtat 4020 tctcaccgat tgtcacgttg gagattcaat agccgtaaat ggggtatgcc ttactgtgac 4080 cgagtttaat aacgactcct tcaaagttgg gatatcacca gaaactataa aacgaagtaa 4140 tgtcgcttcc tggattcaag gcacccaggt caacttggag agagcggtat ctcaagacgt 4200 taggttcggt ggtcattatg tacagggtca cgtagacact gttgctaata ttgtctcaag 4260 aagacctgag gggaattcaa ttatttttgg gtttcagtta agagatcaag agtactttaa 4320 atacatagta gaaaagggat tcatttgtat agatggaact tccttgacca taatcaaggt 4380 tgacccactt tcgcaaggtg gagccttcta tattagtatg ataaagcaca cccaagacaa 4440 tgttatcatg cctttgaaga aaattggcga cgaggttaat attgaagtag atttgactgg 4500 gaagattatt gagaagcaaa ttctattaac gttggaaaac caaatatcaa agaaagatag 4560 tactttgaat actatgatct caaacattat cgaggagaag gttagaaact acctaaataa 4620 ataagcgaat ttcttatgat ttatgatttt tattattaaa taagttataa aaaaaataag 4680 tgtatacaaa ttttaaagtg actcttaggt tttaaaacga aaattcttat tcttgagtaa 4740 ctctttcctg taggtcaggt tgctttctca ggtatagcat gaggtcgctc ttattgacca 4800 cacctctacc ggcaggtgcc ccgggtaacc cagttcctca ctatttttta ctgcggaagc 4860 ggaagcggaa aatacggaaa cgcgcgggaa catacaaaac atacaaaata tacctttctc 4920 acacaagaaa tatatgctac ttgcaaaata tcataccaaa aaacttttca caaccgaaac 4980 caaaaccaac ggatatcata cattacacta ccaccattca aactttacta ctatcctccc 5040 ttcagtttcc ctttttctgc ctttttcggt gacggaaata cgcttcagag accctaaagg 5100 gaaatccatg ccataacagg aaagtaacat cccaatgcgg actataccac cccaccacac 5160 tcctaccaat aacggtaact attctatgtt ttcttactcc tatgtctatt catctttcat 5220 ctgactacct aatactatgc aaaaatgtaa aatcatcaca caaaacataa acaatcaaaa 5280 tcagccattt ccgcaccttt tcctctgtcc actttcaacc gtccctccaa atgtaaaatg 5340 gcctatcgga atacattttc tacatcctaa ctactataaa acaaccttta gacttacgtt 5400 tgctactctc atggtttaaa catcgtcgac ctgcaggcat gcaagcttgg cgtaatcatg 5460 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc 5520 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc 5580 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat 5640 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac 5700 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt 5760 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca 5820 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc 5880 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact 5940 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct 6000 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag 6060 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca 6120 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa 6180 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc 6240 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag 6300 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg 6360 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca 6420 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc 6480 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag 6540 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata 6600 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat 6660 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat 6660 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg 6720 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg 6720 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc 6780 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc 6780 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc 6840 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc 6840 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc 6900 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc 6900 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc 6960 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc 6960 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc 7020 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc 7020 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa 7080 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa 7080 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat 7140 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat 7140 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata 7200 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata 7200 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca 7260 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca 7260 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag 7320 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag 7320 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc 7380 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc 7440 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata 7500 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta 7560 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta 7620 agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg 7680 tc 7682 <210> 17 <211> 9263 <212> DNA <213> Artificial Sequence <400> 17 ggccgcgaca ttgaactctt ggccgactct tctgatgagg ctttcgaaaa atctactttc 60 attaacacaa ttgagttcat tgtgcacgat atttgggtcg aaacgttgat aaagaacata 120 aacttattac aaatgtttga tgcagatttg aaatgctaca atgacaagta cataatttgc 180 aagctaaagg gtcagtatcc ctccacaaat atagtggaca taatgtgccg tttgaagcat 240 ttttccaatt ctattttgga aactttcaaa ttcggaatcg agctaaaaga acaagatcaa 300 tgccacaata gaaacactat tatcaactac gttcttttca gtagagtatt ttcaacgatt 360 gttttagaga ttcaaaaatg ctttatcttg atcgttaagt tcatgtattc tgtcaatttt 420 ttagaaaaat tttccgatga agtatttttg tcatttattg aaattctcat aaaaattgtc 480 tttgagcacc agattccgca gttatttttg ggaattgatg aaattattca actctggtta 540 aagaacaatg aaggaaaacg gcaacaactc ttgagcgctt ggtgcaacgg aactgttcaa 600 gatatgaagc aaagccaaca aagagagtcc tcaaacgcag aatctgaatc aatcacttca 660 agtacagaag aggacgaaga aggcctgcaa ttcaataaat gggatgtgat tgaaccattt 720 attgataata tcaaggcttt gaatcaatca aaatcccata tgtgacgcag cgataaaacc 780 gcggcatgca aacatctaca caattagcaa gggcaatcca tattttgtct tttcgcgccc 840 tggaaaggcc taagtaatgt cgtaaacgca ttctatctgt acttcaactc tcctctgtgc 900 attggtttgt gcaaatcaca ttttacgata ctgccagata tatgcaaaaa gagaaaacca 960 agggaccaga acaaagcaaa attacgatat tcttcgaatt ccttcgtgct tgactaagac 1020 aaagggatgg acgtagcgat ttttagcggg ccaagaactg gttccgaaaa agcacaggta 1080 caccgaaccc tcagctaagg agggacagca ccgatgcgga aggacaaact ttctttttgc 1140 ctatcacagt atcttatcga gctaactatt ttcgacacac atgaaaaagc agaaatatta 1200 acgaaaaaga aaagaaagac catgtcatgt acgggcaatc agaatctgta acaagcgcca 1260 tttttttttc tgtatcgggc cctccttact gctctccttc cgtgtaacgc gttatggctg 1320 caggtcgaca acccttaata taacttcgta taatgtatgc tatacgaagt tattaggtct 1380 agagatctgt ttagcttgcc tcgtccccgc cgggtcaccc ggccagcgac atggaggccc 1440 agaataccct ccttgacagt cttgacgtgc gcagctcagg ggcatgatgt gactgtcgcc 1500 cgtacattta gcccatacat ccccatgtat aatcatttgc atccatacat tttgatggcc 1560 gcacggcgcg aagcaaaaat tacggctcct cgctgcagac ctgcgagcag ggaaacgctc 1620 ccctcacaga cgcgttgaat tgtccccacg ccgcgcccct gtagagaaat ataaaaggtt 1680 aggatttgcc actgaggttc ttctttcata tacttccttt taaaatcttg ctaggataca 1740 gttctcacat cacatccgaa cataaacaac catgggtaaa aagcctgaac tcaccgcgac 1800 gtctgtcgag aagtttctga tcgaaaagtt cgacagcgtc tccgacctga tgcagctctc 1860 ggagggcgaa gaatctcgtg ctttcagctt cgatgtagga gggcgtggat atgtcctgcg 1920 ggtaaatagc tgcgccgatg gtttctacaa agatcgttat gtttatcggc actttgcatc 1980 ggccgcgctc ccgattccgg aagtgcttga cattggggaa ttcagcgaga gcctgaccta 2040 ttgcatctcc cgccgtgcac agggtgtcac gttgcaagac ctgcctgaaa ccgaactgcc 2100 cgctgttctg cagccggtcg cggaggcaat ggatgcgatc gctgcggccg atcttagcca 2160 gacgagcggg ttcggcccat tcggaccgca aggaatcggt caatacacta catggcgtga 2220 tttcatatgc gcgattgctg atccccatgt gtatcactgg caaactgtga tggacgacac 2280 cgtcagtgcg tccgtcgcgc aggctctcga tgagctgatg ctttgggccg aggactgccc 2340 cgaagtccgg cacctcgtgc acgcggattt cggctccaac aatgtcctga cggacaatgg 2400 ccgcataaca gcggtcattg actggagcga ggcgatgttc ggggattccc aatacgaggt 2460 cgccaacatc ttcttctgga ggccgtggtt ggcttgtatg gagcagcaga cgcgctactt 2520 cgagcggagg catccggagc ttgcaggatc gccgcggctc cgggcgtata tgctccgcat 2580 tggtcttgac caactctatc agagcttggt tgacggcaat ttcgatgatg cagcttgggc 2640 gcagggtcga tgcgacgcaa tcgtccgatc cggagccggg actgtcgggc gtacacaaat 2700 cgcccgcaga agcgcggccg tctggaccga tggctgtgta gaagtactcg ccgatagtgg 2760 aaaccgacgc cccagcactc gtccgagggc aaaggaataa tcagtactga caataaaaag 2820 attcttgttt tcaagaactt gtcatttgta tagttttttt atattgtagt tgttctattt 2880 taatcaaatg ttagcgtgat ttatattttt tttcgcctcg acatcatctg cccagatgcg 2940 aagttaagtg cgcagaaagt aatatcatgc gtcaatcgta tgtgaatgct ggtcgctata 3000 ctgctgtcga ttcgatacta acgagctcat agcttcaaaa tgtttctact ccttttttac 3060 tcttccagat tttctcggac tccgcgcatc gccgtaccac ttcaaaacac ccaagcacag 3120 catactaaat ttcccctctt tcttcctcta gggtgtcgtt aattacccgt actaaaggtt 3180 tggaaaagaa aaaagagacc gcctcgtttc tttttcttcg tcgaaaaagg caataaaaat 3240 ttttatcacg tttctttttc ttgaaaattt tttttttgat ttttttctct ttcgatgacc 3300 tcccattgat atttaagtta ataaacggtc ttcaatttct caagtttcag tttcattttt 3360 cttgttctat tacaactttt tttacttctt gctcattaga aagaaagcat agcaatctaa 3420 tctaagtttt ctagaactag tggatccccc gggaaaaatg gacaagaagt actccattgg 3480 gctcgatatc ggcacaaaca gcgtcggttg ggccgtcatt acggacgagt acaaggtgcc 3540 gagcaaaaaa ttcaaagttc tgggcaatac cgatcgccac agcataaaga agaacctcat 3600 tggcgccctc ctgttcgact ccggggagac ggccgaagcc acgcggctca aaagaacagc 3660 acggcgcaga tatacccgca gaaagaatcg gatctgctac ctgcaggaga tctttagtaa 3720 tgagatggct aaggtggatg actctttctt ccataggctg gaggagtcct ttttggtgga 3780 ggaggataaa aagcacgagc gccacccaat ctttggcaat atcgtggacg aggtggcgta 3840 ccatgaaaag tacccaacca tatatcatct gaggaagaag cttgtagaca gtactgataa 3900 ggctgacttg cggttgatct atctcgcgct ggcgcatatg atcaaatttc ggggacactt 3960 cctcatcgag ggggacctga acccagacaa cagcgatgtc gacaaactct ttatccaact 4020 ggttcagact tacaatcagc ttttcgaaga gaacccgatc aacgcatccg gagttgacgc 4080 caaagcaatc ctgagcgcta ggctgtccaa atcccggcgg ctcgaaaacc tcatcgcaca 4140 gctccctggg gagaagaaga acggcctgtt tggtaatctt atcgccctgt cactcgggct 4200 gacccccaac tttaaatcta acttcgacct ggccgaagat gccaagcttc aactgagcaa 4260 agacacctac gatgatgatc tcgacaatct gctggcccag atcggcgacc agtacgcaga 4320 cctttttttg gcggcaaaga acctgtcaga cgccattctg ctgagtgata ttctgcgagt 4380 gaacacggag atcaccaaag ctccgctgag cgctagtatg atcaagcgct atgatgagca 4440 ccaccaagac ttgactttgc tgaaggccct tgtcagacag caactgcctg agaagtacaa 4500 ggaaattttc ttcgatcagt ctaaaaatgg ctacgccgga tacattgacg gcggagcaag 4560 ccaggaggaa ttttacaaat ttattaagcc catcttggaa aaaatggacg gcaccgagga 4620 ccaggaggaa ttttacaaat ttattaagcc catcttggaa aaaatggacg gcaccgagga 4620 gctgctggta aagcttaaca gagaagatct gttgcgcaaa cagcgcactt tcgacaatgg 4680 gctgctggta aagcttaaca gagaagatct gttgcgcaaa cagcgcactt tcgacaatgg 4680 aagcatcccc caccagattc acctgggcga actgcacgct atcctcaggc ggcaagagga 4740 aagcatcccc caccagattc acctgggcga actgcacgct atcctcaggc ggcaagagga 4740 tttctacccc tttttgaaag ataacaggga aaagattgag aaaatcctca catttcggat 4800 tttctacccc tttttgaaag ataacaggga aaagattgag aaaatcctca catttcggat 4800 accctactat gtaggccccc tcgcccgggg aaattccaga ttcgcgtgga tgactcgcaa 4860 accctactat gtaggccccc tcgcccgggg aaattccaga ttcgcgtgga tgactcgcaa 4860 atcagaagag accatcactc cctggaactt cgaggaagtc gtggataagg gggcctctgc 4920 atcagaagag accatcactc cctggaactt cgaggaagtc gtggataagg gggcctctgc 4920 ccagtccttc atcgaaagga tgactaactt tgataaaaat ctgcctaacg aaaaggtgct 4980 ccagtccttc atcgaaagga tgactaactt tgataaaaat ctgcctaacg aaaaggtgct 4980 tcctaaacac tctctgctgt acgagtactt cacagtttat aacgagctca ccaaggtcaa 5040 tcctaaacac tctctgctgt acgagtactt cacagtttat aacgagctca ccaaggtcaa 5040 atacgtcaca gaagggatga gaaagccagc attcctgtct ggagagcaga agaaagctat 5100 atacgtcaca gaagggatga gaaagccagc attcctgtct ggagagcaga agaaagctat 5100 cgtggacctc ctcttcaaga cgaaccggaa agttaccgtg aaacagctca aagaagacta 5160 cgtggacctc ctcttcaaga cgaaccggaa agttaccgtg aaacagctca aagaagacta 5160 tttcaaaaag attgaatgtt tcgactctgt tgaaatcagc ggagtggagg atcgcttcaa 5220 tttcaaaaag attgaatgtt tcgactctgt tgaaatcagc ggagtggagg atcgcttcaa 5220 cgcatccctg ggaacgtatc acgatctcct gaaaatcatt aaagacaagg acttcctgga 5280 cgcatccctg ggaacgtatc acgatctcct gaaaatcatt aaagacaagg acttcctgga 5280 caatgaggag aacgaggaca ttcttgagga cattgtcctc acccttacgt tgtttgaaga 5340 tagggagatg attgaagaac gcttgaaaac ttacgctcat ctcttcgacg acaaagtcat 5400 gaaacagctc aagaggcgcc gatatacagg atgggggcgg ctgtcaagaa aactgatcaa 5460 tgggatccga gacaagcaga gtggaaagac aatcctggat tttcttaagt ccgatggatt 5520 tgccaaccgg aacttcatgc agttgatcca tgatgactct ctcaccttta aggaggacat 5580 ccagaaagca caagtttctg gccaggggga cagtcttcac gagcacatcg ctaatcttgc 5640 aggtagccca gctatcaaaa agggaatact gcagaccgtt aaggtcgtgg atgaactcgt 5700 caaagtaatg ggaaggcata agcccgagaa tatcgttatc gagatggccc gagagaacca 5760 aactacccag aagggacaga agaacagtag ggaaaggatg aagaggattg aagagggtat 5820 aaaagaactg gggtcccaaa tccttaagga acacccagtt gaaaacaccc agcttcagaa 5880 tgagaagctc tacctgtact acctgcagaa cggcagggac atgtacgtgg atcaggaact 5940 ggacatcaat cggctctccg actacgacgt ggatcatatc gtgccccagt cttttctcaa 6000 agatgattct attgataata aagtgttgac aagatccgat aaaaatagag ggaagagtga 6060 taacgtcccc tcagaagaag ttgtcaagaa aatgaaaaat tattggcggc agctgctgaa 6120 cgccaaactg atcacacaac ggaagttcga taatctgact aaggctgaac gaggtggcct 6180 gtctgagttg gataaagccg gcttcatcaa aaggcagctt gttgagacac gccagatcac 6240 caagcacgtg gcccaaattc tcgattcacg catgaacacc aagtacgatg aaaatgacaa 6300 actgattcga gaggtgaaag ttattactct gaagtctaag ctggtctcag atttcagaaa 6360 ggactttcag ttttataagg tgagagagat caacaattac caccatgcgc atgatgccta 6420 cctgaatgca gtggtaggca ctgcacttat caaaaaatat cccaagcttg aatctgaatt 6480 tgtttacgga gactataaag tgtacgatgt taggaaaatg atcgcaaagt ctgagcagga 6540 aataggcaag gccaccgcta agtacttctt ttacagcaat attatgaatt ttttcaagac 6600 cgagattaca ctggccaatg gagagattcg gaagcgacca cttatcgaaa caaacggaga 6660 aacaggagaa atcgtgtggg acaagggtag ggatttcgcg acagtccgga aggtcctgtc 6720 catgccgcag gtgaacatcg ttaaaaagac cgaagtacag accggaggct tctccaagga 6780 aagtatcctc ccgaaaagga acagcgacaa gctgatcgca cgcaaaaaag attgggaccc 6840 caagaaatac ggcggattcg attctcctac agtcgcttac agtgtactgg ttgtggccaa 6900 agtggagaaa gggaagtcta aaaaactcaa aagcgtcaag gaactgctgg gcatcacaat 6960 catggagcga tcaagcttcg aaaaaaaccc catcgacttt ctcgaggcga aaggatataa 7020 agaggtcaaa aaagacctca tcattaagct tcccaagtac tctctctttg agcttgaaaa 7080 cggccggaaa cgaatgctcg ctagtgcggg cgagctgcag aaaggtaacg agctggcact 7140 gccctctaaa tacgttaatt tcttgtatct ggccagccac tatgaaaagc tcaaagggtc 7200 tcccgaagat aatgagcaga agcagctgtt cgtggaacaa cacaaacact accttgatga 7260 gatcatcgag caaataagcg aattctccaa aagagtgatc ctcgccgacg ctaacctcga 7320 taaggtgctt tctgcttaca ataagcacag ggataagccc atcagggagc aggcagaaaa 7380 cattatccac ttgtttactc tgaccaactt gggcgcgcct gcagccttca agtacttcga 7440 caccaccata gacagaaagc ggtacacctc tacaaaggag gtcctggacg ccacactgat 7500 tcatcagtca attacggggc tctatgaaac aagaatcgac ctctctcagc tcggtggaga 7560 cagcagggct gaccccaaga agaagaggaa ggtgtgatct cttctcgagt catgtaatta 7620 gttatgtcac gcttacattc acgccctccc cccacatccg ctctaaccga aaaggaagga 7680 gttagacaac ctgaagtcta ggtccctatt tattttttta tagttatgtt agtattaaga 7740 acgttattta tatttcaaat ttttcttttt tttctgtaca gacgcgtgta cgcatgtaac 7800 attatactga aaaccttgct tgagaaggtt ttgggacgct cgaaggcttt aatttgcccg 7860 ccatccagtg tcgaaaacga gctctcgaga acccttaata taacttcgta taatgtatgc 7920 tatacgaagt tattaggtga tatcagatcc actagtggct actatcggcg actctctcga 7980 aatttttctt aacgcgtcct tgtactgcgt ctaacgcttt tgccacttgg atttctatta 8040 taggaaatag tctcacttac tgggcgacga attttcgcgt tttgatgaag cacaggaaga 8100 atttcttttt tttttggctt cttctggttc cgttttttac gcgcacaaat ctaaaaaaag 8160 aaataattat aacctagtct cgaaaatttt catcgatcca ttcgttcctt tttttcgatt 8220 ttttcagatc aaaattcttg tttctttctt tgtcttagtt tatattaaaa gatattttga 8280 ttttactcct gaactattta ttctttctaa gaaggccaga acactacagc tgttttaacc 8340 gactacgaag ttctccattc tcgaacacta gccttcattt accaaacagg aactagcgta 8400 tatcattagt ccttattcga aaagagattg gtagatattt attgtagttt gtgagaagga 8460 gaaaatactg tcattggact gatagttaga ggacattaac ctctcttacg ttcgctcaaa 8520 aaaattaaaa taagcaagta gctgaaatca agtctttcat acaacggtca gaccatgaac 8580 actgatcaac aaccttatca gggccaaacg gactataccc agggaccagg taacgggcaa 8640 agtcaggaac aagactatga ccaatatggc cagcctttgt atccttcaca agctgatggt 8700 tactacgatc caaatgtcgc tgctggtact gaagctgata tgtatggtca acaaccacca 8760 aacgagtctt acgaccaaga ctacacaaac ggtgaatact atggtcaacc gccaaatatg 8820 gctgctcaag acggtgaaaa cttctcggat tttagcagtt acggccctcc tggaacacct 8880 ggatatgata gctatggtgg tcagtatacc gcttctcaaa tgagttatgg agaaccaaat 8940 tcgtcgggta cctcgactcc aatttacggt aattatgacc caaatgctat cgctatggct 9000 ttgccaaatg aaccttatcc cgcttggact gctgactctc aatctcccgt ttcgatcgag 9060 caaatcgaag atatctttat tgatttgacc aacagactcg ggttccaaag agactccatg 9120 agaaatatgt ttgatcattt tatggttctc ttggactcta ggtcctcgag aatgtctcct 9180 gatcaagctt tactatcttt acatgccgac tacattggtg gcgatactgc taactataaa 9240 aaatggtatt ttgctgctca ggc 9263 <210> 18 <211> 8424 <212> DNA <213> Artificial Sequence <400> 18 ggccgcgcag agaatcctcg ggtttgtaaa ccatgttata gttaaacccc gatattacac 60 cattaaaatc atttctaaat atgcggtcgt cgatgatcag ctgcagggaa atgccaaaat 120 atggacccca tagaggtctt ggcttgttca tatcgttggg tcgatttctg ttcccagtgt 180 tatttggttg aatgggaaca gtcgcgtctt gccccatctt gtctctgccg ctttcgtcga 240 atctgacttt gcaattgacg ttgcatatca acgtgccgga tcccggaata gcgtgatagt 300 ctactcctgt tagggcatgt tgtgtttgta cgacctggtt ttgccacatt tgcagaaaaa 360 ctgttgcttg cgcgaagggg gtagcattaa atattattct gcagttgttt ggattaaaaa 420 gctgtacgaa ttgggccaat ttgttggagt ccggatcatc caaatgcgct aatatcttct 480 tcgtaaatgt ggccgttatt tgtgcttgat ttgcgtcatt cattaccata gtattactat 540 tggtgttcat atttctgtta ttactgttat tagtttggcg ttttgcctga cgatcagcct 600 ggtggggcca agtatatact ataaaaacag gttagttact tgctctatgc gtttgcgcat 660 cctcttttta cttttttttt ttcagtaaag cctaagcata aatcgtttta tacgtacgac 720 acgttcaact tttcttggtt agtagtggca atctctgcaa tacatacagg gagtcatggt 780 ctatcatctt gtccaatcaa agaagcatcg gttcagatcg agcaaactgt agggagaaag 840 gaaagtagaa atgcagagtg tgctatatgt ccaatctcgg ttttgtagtt tggatgtcat 900 tagagatcta ccacccaacc ggctgctttc atgtggaaca gaaaagaaat cggggcgctt 960 cctcttctgt attcctttaa ttaacgtttt tattcagcca tctaaccatc atacccccat 1020 acggtaacaa aacctcttct aagaaaagaa gtctctgctc ctccgccatc ttatttttat 1080 tcgctgcgcg cgtttattgt cgcatcgcta gccagcaaaa agttggttgc ctttttttac 1140 ctaaaaaaga cacatctaac tgattagttt tccgttttag gatattgacg ccaagcgtgc 1200 gtctgattgc aaattaaagc cttcgagcgt cccaaaacct tctcaagcaa ggttttcagt 1260 ataatgttac atgcgtacac gcgtttgtac agaaaaaaaa gaaaaatttg aaatataaat 1320 aacgttctta atactaacat aactataaaa aaataaatag ggacctagac ttcaggttgt 1380 ctaactcctt ccttttcggt tagagcggat gtggggggag ggcgtgaatg taagcgtgac 1440 ataactaatt acatgatatc gacaaaggaa aaggggcctg tttatttatc aagataagtt 1500 tccggatctt tttctttcct aacaccccag tcagcctgag ttacatccag ccattgaacc 1560 ttagaaaatc ttttgtcatc agcggtttga gccctaagat caacatcttg cttagcaatc 1620 actgcaatgg cgtcataacc accagcacca ggtattaagc aagtaagaac tccttttaag 1680 gtctggcaat catccaataa gctagtttgt acgggaggtt cgatatcggc accagattct 1740 ttagttattt ttctaaagga acgtctaatt gtggcaactg catctctaac ttctgtgatc 1800 tcaggatact tttgacaggt acagtcattc ctctcaagag actcaaatat ctgatcgctg 1860 taatcgtcat gagtctcgtg taagcgatct agtttagata gtccatccat aaatctagaa 1920 tttgcatgat cgagttctgt atatattttc aagctttccg gcatatgcga atcataccaa 1980 ttttttacct tctggaccag ttttactgtt tctgaaccat tcttaatatc gcccatccat 2040 aaagttaatc ccgaaggtaa atggttactt ttaatcgtta tattccagtc ttcttcatta 2100 accaaatgcg ccagtttact gccgtaagta gcacttccaa tatctggcaa attagagatt 2160 aatgcgggtg ggaatcttct atatctgata gatccatatg ctgccgccgc tacatcaaac 2220 ccgcttccaa ttttaccctg agcttgacaa tgagcaactt gtgataaatt atgaataact 2280 tctctatatt tgtctacatt attttccagg tccgatacaa aaaaggaggc caaagctgta 2340 gttaaaactg tgactaaacc tgccgaggag cccagccctg ttttgggaac ttcttcaatt 2400 ctgtgcgaat gaaaactcaa tcttctgttg ccacgatgtt cggtaacgct gtcctcctga 2460 ctgtgcgaat gaaaactcaa tcttctgttg ccacgatgtt cggtaacgct gtcctcctga 2460 gaatggtagg catcatcaga gaaaatatca ataacgaaca agtttctatt gcagtagtcg 2520 gaatggtagg catcatcaga gaaaatatca ataacgaaca agtttctatt gcagtagtcg 2520 tccatgttag gcttaaagta gctaaatacg ttagcgataa ctttttcaat gaaagggttc 2580 tccatgttag gcttaaagta gctaaatacg ttagcgataa ctttttcaat gaaagggttc 2580 ttagatccgc ctatcgaaac aggaatgaag ccagttttag gacttatatg gtacagccac 2640 ttagatccgc ctatcgaaac aggaatgaag ccagttttag gacttatatg gtacagccac 2640 tccccatctt taaattgttt acttttcaca cgcacttcaa acttatcaga ctcttgcaat 2700 tccccatctt taaattgttt acttttcaca cgcacttcaa acttatcaga ctcttgcaat 2700 gaaccgtaag gatgggctac agcatgcatt cttgccgata atccgactac aaatgcttca 2760 gaaccgtaag gatgggctac agcatgcatt cttgccgata atccgactac aaatgcttca 2760 tatttcggat ctaaaactaa atatccacca gctagtaacg ctttccctgg ggcactgaag 2820 tatttcggat ctaaaactaa atatccacca gctagtaacg ctttccctgg ggcactgaag 2820 gctctcaact ctgacattat agttttttct ccttgacgtt aaagtataga ggtatattaa 2880 gctctcaact ctgacattat agttttttct ccttgacgtt aaagtataga ggtatattaa 2880 caattttttg ttgatacttt tatgacattt gaataagaag taatacaaac cgaaaatgtt 2940 caattttttg ttgatacttt tatgacattt gaataagaag taatacaaac cgaaaatgtt 2940 gaaagtatta gttaaagtgg ttatgcagct tttgcattta tatatctgtt aatagatcaa 3000 gaaagtatta gttaaagtgg ttatgcagct tttgcattta tatatctgtt aatagatcaa 3000 aaatcatcgc ttcgctgatt aattacccca gaaataaggc taaaaaacta atcgcattat 3060 aaatcatcgc ttcgctgatt aattacccca gaaataaggc taaaaaacta atcgcattat 3060 tatcctatgg ttgttaattt gattcgttga tttgaaggtt tgtggggcca ggttactgcc 3120 tatcctatgg ttgttaattt gattcgttga tttgaaggtt tgtggggcca ggttactgcc 3120 aatttttcct cttcataacc ataaaagcta gtattgtaga atctttattg ttcggagcag 3180 tgcggcgcga ggcacatctg cgtttcagga acgcgaccgg tgaagaccag gacgcacgga 3240 ggagagtctt ccgtcggagg gctgtcgccc gctcggcggc ttctaatccg tacttcaata 3300 tagcaatgag cagttaagcg tattactgaa agttccaaag agaaggtttt tttaggctaa 3360 gataatgggg ctctttacat ttccacaaca tataagtaag attagatatg gatatgtata 3420 tggtggtatt gccatgtaat atgattatta aacttctttg cgtccatcca aaaaaaaagt 3480 aagaattttt gaaaattcaa tataaatggt tttaaccaat aaaacagtca tttctggatc 3540 gaaagtcaaa agtttatcat ctgcgcaatc gagctcatca ggaccttcat catctagtga 3600 ggaagatgat tcccgcgata ttgaaagctt ggataagaaa atacgtcctt tagaagaatt 3660 agaagcatta ttaagtagtg gaaatacaaa acaattgaag aacaaagagg tcgctgcctt 3720 ggttattcac ggtaagttac ctttgtacgc tttggagaaa aaattaggtg atactacgag 3780 agcggttgcg gtacgtagga aggctctttc aattttggca gaagctcctg tattagcatc 3840 tgatcgttta ccatataaaa attatgacta cgaccgcgta tttggcgctt gttgtgaaaa 3900 tgttataggt tacatgcctt tgcccgttgg tgttataggc cccttggtta tcgatggtac 3960 atcttatcat ataccaatgg caactacaga gggttgtttg gtagcttctg ccatgcgtgg 4020 ctgtaaggca atcaatgctg gcggtggtgc aacaactgtt ttaactaagg atggtatgac 4080 aagaggccca gtagtccgtt tcccaacttt gaaaagatct ggtgcctgta agatatggtt 4140 agactcagaa gagggacaaa acgcaattaa aaaagctttt aactctacat caagatttgc 4200 acgtctgcaa catattcaaa cttgtctagc aggagattta ctcttcatga gatttagaac 4260 aactactggt gacgcaatgg gtatgaatat gatttctaaa ggtgtcgaat actcattaaa 4320 gcaaatggta gaagagtatg gctgggaaga tatggaggtt gtctccgttt ctggtaacta 4380 ctgtaccgac aaaaaaccag ctgccatcaa ctggatcgaa ggtcgtggta agagtgtcgt 4440 cgcagaagct actattcctg gtgatgttgt cagaaaagtg ttaaaaagtg atgtttccgc 4500 attggttgag ttgaacattg ctaagaattt ggttggatct gcaatggctg ggtctgttgg 4560 tggatttaac gcacatgcag ctaatttagt gacagctgtt ttcttggcat taggacaaga 4620 tcctgcacaa aatgttgaaa gttccaactg tataacattg atgaaagaag tggacggtga 4680 tttgagaatt tccgtatcca tgccatccat cgaagtaggt accatcggtg gtggtactgt 4740 tctagaacca caaggtgcca tgttggactt attaggtgta agaggcccgc atgctaccgc 4800 tcctggtacc aacgcacgtc aattagcaag aatagttgcc tgtgccgtct tggcaggtga 4860 attatcctta tgtgctgccc tagcagccgg ccatttggtt caaagtcata tgacccacaa 4920 caggaaacct gctgaaccaa caaaacctaa caatttggac gccactgata taaatcgttt 4980 gaaagatggg tccgtcacct gcattaaatc ctaatttgcc agcttactat ccttcttgaa 5040 aatatgcact ctatatcttt tagttcttaa ttgcaacaca tagatttgct gtataacgaa 5100 ttttatgcta ttttttaaat ttggagttca gtgataaaag tgtcacagcg aatttcctca 5160 catgtaggga ccgaattgtt tacaagttct ctgtaccacc atggagacat caaagattga 5220 aaatctatgg aaagatatgg acggtagcaa caagaatata gcacgagccg cgaagttcat 5280 ttcgttactt ttgatatcgc tcacaactat tgcgaagcgc ttcagtgaaa aaatcataag 5340 gaaaagttgt aaatattatt ggtagtattc gtttggtaaa gtagaggggg taatttttcc 5400 cctttatttt gttcatacat tcttaaattg ctttgcctct ccttttggaa agctatactt 5460 cggagcactg ttgagcgaag gctcattaga tatattttct gtcattttcc ttaacccaaa 5520 aataagggaa agggtccaaa aagcgctcgg acaactgttg accgtgatcc gaaggactgg 5580 ctatacagtg ttcacaaaat agccaagctg aaaataatgt gtagctatgt tcagttagtt 5640 tggctagcaa agatataaaa gcaggtcgga aatatttatg ggcattatta tgcagagcat 5700 caacatgata aaaaaaaaca gttgaatatt ccctcaaaaa tgtcattacc gttcttaact 5760 tctgcaccgg gaaaggttat tatttttggt gaacactctg ctgtgtacaa caagcctgcc 5820 gtcgctgcta gtgtgtctgc gttgagaacc tacctgctaa taagcgagtc atctgcacca 5880 gatactattg aattggactt cccggacatt agctttaatc ataagtggtc catcaatgat 5940 ttcaatgcca tcaccgagga tcaagtaaac tcccaaaaat tggccaaggc tcaacaagcc 6000 accgatggct tgtctcagga actcgttagt cttttggatc cgttgttagc tcaactatcc 6060 gaatccttcc actaccatgc agcgttttgt ttcctgtata tgtttgtttg cctatgcccc 6120 catgccaaga atattaagtt ttctttaaag tctactttac ccatcggtgc tgggttgggc 6180 tcaagcgcct ctatttctgt atcactggcc ttagctatgg cctacttggg ggggttaata 6240 ggatctaatg acttggaaaa gctgtcagaa aacgataagc atatagtgaa tcaatgggcc 6300 ttcataggtg aaaagtgtat tcacggtacc ccttcaggaa tagataacgc tgtggccact 6360 tatggtaatg ccctgctatt tgaaaaagac tcacataatg gaacaataaa cacaaacaat 6420 tttaagttct tagatgattt cccagccatt ccaatgatcc taacctatac tagaattcca 6480 aggtctacaa aagatcttgt tgctcgcgtt cgtgtgttgg tcaccgagaa atttcctgaa 6540 gttatgaagc caattctaga tgccatgggt gaatgtgccc tacaaggctt agagatcatg 6600 actaagttaa gtaaatgtaa aggcaccgat gacgaggctg tagaaactaa taatgaactg 6660 tatgaacaac tattggaatt gataagaata aatcatggac tgcttgtctc aatcggtgtt 6720 tctcatcctg gattagaact tattaaaaat ctgagcgatg atttgagaat tggctccaca 6780 aaacttaccg gtgctggtgg cggcggttgc tctttgactt tgttacgaag agacattact 6840 caagagcaaa ttgacagctt caaaaagaaa ttgcaagatg attttagtta cgagacattt 6900 gaaacagact tgggtgggac tggctgctgt ttgttaagcg caaaaaattt gaataaagat 6960 cttaaaatca aatccctagt attccaatta tttgaaaata aaactaccac aaagcaacaa 7020 attgacgatc tattattgcc aggaaacacg aatttaccat ggacttcata agcgaatttc 7080 ttatgattta tgatttttat tattaaataa gttataaaaa aaataagtgt atacaaattt 7140 taaagtgact cttaggtttt aaaacgaaaa ttcttattct tgagtaactc tttcctgtag 7200 gtcaggttgc tttctcaggt atagcatgag gtcgctctta ttgaccacac ctctaccggt 7260 tacgtggatt gagccagcaa tacagatcat tattaaactg ttttgtacat gatgttagta 7320 tataatcgta aagcttttct aatatgtata ccttatacat ggaactccac agaacttgca 7380 aacataccaa aaatccttta ttcttgttca ctcattttac atcaaaaaat aatatttcag 7440 ttattaagga aaataaaaaa atagattaga gaagcatttt gaagaaatag tatattcttt 7500 tattgaacct aagagcgtga tatttttact cgaaataaaa tacgaaaaat ctatacactc 7560 atctttccga ctactattgg ctcctgctca aaaaaagagg gaaaaaaagc tccaaaattc 7620 tatcttttcc tatcgctcct gtcctatcct tattacgttc attactattt taatactatc 7680 cattctttta ttttcagtct aaaaaaaaca tttctcataa cgggaaaagc aaaaaaatgt 7740 caagcttata catcaaaaca ccactgcatg cattatctgc tggtccggat tctcatgcaa 7800 atagctccta ttatgacaat cttttattgc cttcattttc caatttatcc agtaatatca 7860 gcaggaacaa tatcacaacc gataacaata taaattcagc aagtcctagg aagtattcgt 7920 ttcattcgct caatgtgtcg ccaattcttt ccccaatatc tttggctaac gaaatacttg 7980 gaaaaaaatc aaatactgca ccagcatcgc cacatcatat ggattacaat ccaatttcct 8040 cattaacacc aggcaattca ccagaattca acaaggcaag cttatctcag attagtttta 8100 caaatccatt gaactatggg tctggcttag gtttttcctc taattcacaa cctcgactac 8160 cattgttaga caggctatcg tccgtctctt tatctaagag accggagcgc ccacaacaaa 8220 gcctaccatc actaaggcat ctgcaattat tacccagtcc tcttttacaa gaaaacgcag 8280 cacgcttccc tgatacatca aaacgtacat ctaattggaa aactgatctt acccattggt 8340 gtaaggatac aaactatcaa gactatgtta aaatacgcga agaagtggct catttcaagc 8400 cattaagtat acccaactta acgc 8424 <210> 19 <211> 7396 <212> DNA <213> Artificial Sequence <400> 19 ggccgcgact caatgacttc gggatctaat tcagcataaa taatctcttc tccctcgcca 60 gcttcagcta caatcttgcc tctggggtcc accactatag agtggccata tgcatggtaa 120 gaactctgta gattccttgc gggagaacat aacatcacgt acacttggtt gtcaactgcc 180 ctgcttcttg ccagtaaatg ccaatgcaag ggccctgtga cagtgttgaa tgcactaggg 240 tagatcatgg caaacgcacc cttgcgtgca cttagcattg cgagttccgg aaacctcatg 300 tcatagcata tacctacgcc aaacttacca tatttcgtgt caatggtagt agacttctct 360 ccagggctca aggtttcact ttcatggaac gatataccgt taggaatgtc tacatcgaaa 420 agatggacct tccgatgctt gtcaatcaac ttgccatcct cgttgaaaat tatagaggtg 480 ttgtagattt tgtctgtctt tggatccaat tccggaattg ttcctcctac caagatgatt 540 ttaaacttat tggctaaatt agacagaaat tgaactgacg tcgaaggctc tttcggattg 600 ataacttctg aatactttct aaactgatca gtggagtacg gagaattgaa gcattctggc 660 aatacgacca acttggtatc cggctgttcc ttcatagccc tttcaatgaa cgttgcagcc 720 ctttgaagat tggccatttt gtcaggactc gagcctgaca gttggaccaa cgcaacttta 780 attttttgtg aaagaatctt cgaagcactc atactggcga tcttcacgcc ctcctgctat 840 tacaaaagct gtgtttttac aagaatcaaa ttaagttagc aagatatcat acaacattat 900 tgataatttc aatatcgtgt tcgtacctga tgacgtatct gtgcattgat aaggcccgca 960 tggtttcaga aagcagagcg gaacgattcc aaattagtgg ccttgtgctt tgcatgtcaa 1020 ttgtgttacc ttcagctcgt ggatttgttt tatcaataca cagtctacag tcaagnnnnn 1080 nnnnntatca aattttgcgt tcgagcgtat aaaatagccg ctgtagctac ttaagttcct 1140 gttcagccgg tagaggtgtg gtcaataaga gcgacctcat gctatacctg agaaagcaac 1200 ctgacctaca ggaaagagtt actcaagaat aagaattttc gttttaaaac ctaagagtca 1260 ctttaaaatt tgtatacact tatttttttt ataacttatt taataataaa aatcataaat 1320 cataagaaat tcgcttattc ctttggtaga ccagtctttg cgtcaatcaa agattcgttt 1380 gtttcttgtg ggcctgaacc gacttgagtt aaaatcactc tggcaacatc cttttgcaac 1440 tcaagatcca attcacgtgc agtaaagtta gatgattcaa attgatggtt gaaagcctca 1500 agctgctcag tagtaaattt cttgtcccat ccaggaacag agccaaacaa tttatagata 1560 aatgcaaaga gtttcgactc attttcagct aagtagtaca acacagcatt tggacctgca 1620 tcaaacgtgt atgcaacgat tgtttctccg taaaactgat taatggtgtg gcaccaactg 1680 atgatacgct tggaagtgtc attcatgtag aatattggag ggaaagagtc caaacatgtg 1740 gcatggaaag agttggaatc catcattgtt tcctttgcaa aggtggcgaa atctttttca 1800 acaatggctt tacgcatgac ttcaaatctc tttggtacga catgttcaat tctttcttta 1860 aatagttcgg aggttgccac ggtcaattgc ataccctgag tggaactcac atccttttta 1920 atatcgctga caactaggac acaagctttc atctgaggcc agtcagagct gtctgcgatt 1980 tgtactgcca tggaatcatg accatcttca gcttttccca tttcccaggc cacgtatccg 2040 ccaaacaacg atctacaagc tgaaccagac ccctttcttg ctattctaga tatttctgaa 2100 gttgactgtg gtaattggta taacttagca attgcagaga ccaatgcagc aaagccagca 2160 gcggaggaag ctaaaccagc tgctgtagga aagttatttt cggagacaat gtggagtttc 2220 cattgagata atgtgggcaa tgaggcgtcc ttcgattcca tttcctttct taattggcgt 2280 aggtcgcgca gacaattttg agttctttca ttgtcgatgc tgtgtggttc tccatttaac 2340 cacaaagtgt cgcgttcaaa ctcaggtgca gtagccgcag aggtcaacgt tctgaggtca 2400 tcttgcgata aagtcactga tatggacgaa ttggtgggca gattcaactt cgtgtccctt 2460 ttcccccaat acttaagggt tgcgatgttg acgggtgcgg taacggatgc tgtgtaaacg 2520 gtcatttttg agggaatatt caactgtttt tttttatcat gttgatgctc tgcataataa 2580 tgcccataaa tatttccgac ctgcttttat atctttgcta gccaaactaa ctgaacatag 2640 ctacacatta ttttcagctt ggctattttg tgaacactgt atagccagtc cttcggatca 2700 cggtcaacag ttgtccgagc gctttttgga ccctttccct tatttttggg ttaaggaaaa 2760 tgacagaaaa tatatctaat gagccttcgc tcaacagtgc tccgaagtat agctttccaa 2820 aaggagaggc aaagcaattt aagaatgtat gaacaaaata aaggggaaaa attaccccct 2880 ctactttacc aaacgaatac taccaataat atttacaact tttccttatg attttttcac 2940 tgaagcgctt cgcaatagtt gtgagcgata tcaaaagtaa cgaaatgaac ttcgcggctc 3000 gtgctatatt cttgttgcta ccgtccatat ctttccatag attttcaatc tttgatgtct 3060 ccatggtggt acagagaact tgtaaacaat tcggtcccta catgtgagga aattcgctgt 3120 gacactttta tcactgaact ccaaatttaa aaaatagcat aaaattcgtt atacagcaaa 3180 tctatgtgtt gcaattaaga actaaaagat atagagtgca tattttcaag aaggatagta 3240 agctggcaaa tcatatcttt tcaatgacaa tagaggaagc accaccacca ccattacaaa 3300 tggcggcaac accgatctta cctccttctt gctgtaagat ggatagcagt gtaacaacca 3360 ctctagcacc agaacaaccc aatgggtgac ctagagcaac agcaccacca tatacattaa 3420 ccttagatgg gtctagcttc aaaatcttag tgttcaccaa accgacaacc gaaaaggctt 3480 cattgaattc aaagtaatca acagaattga tgtcttcgat gccagcatgt ttcaaagcct 3540 ttggaactgc aagagatgga gcccatgtaa aatcagctgg ttgatgagcg gcctcacccc 3600 aacctttgat aatagccaaa ggcttcaaat tcttttcctt caaaactttt tcggaaacca 3660 agatgacggc tgcagcacca tcgttgattg gagaagcgtt agcggcagta acagtaccgt 3720 tttctttttg gaaaacagtc cttgcagatc tcaatttttc aacgtgtaat ctagcaggtt 3780 cctcgtcctt cgtgacttga gtatcaggct tacctctaaa tcccttaatg gtaacaggta 3840 caatttcatt gtcgaattta ccttcctttt gagatttttg agatttttgg taggattcga 3900 tggcaaaatt gtcttgttgt tctctagtaa tatcccaatc acgggcacac ttttctgcgt 3960 gtacacccat ggctagacca tcgtacgcat cgttcaaccc atctctttcg acaccatcaa 4020 caagaacagt ttggccaaat ttggcacccg cacgggctgc tggcatgtag tatggtgcgt 4080 tagtcataga ttcacaacca ccagctacga caacatcagc attaccacat ttgatggatt 4140 gagcacccaa aatgattgcc ttcatagcgg atgcacagac cttgttaact gtgcttgcaa 4200 cgatatgatt actcaaaccg gcagccaaag caacttgtct ggccggagct tggcccaaat 4260 tggcagaaag aacgttacca aaaataattt cgtcaaaatc cttggatgca tccaattctg 4320 gaaccttagc caaggcgcct tttaaagcaa cagcacccaa ttccactgct gtcttggagg 4380 atagagaacc ctggaatgaa ccaattgggg ttctggcagt cgatacaatg taaacgttct 4440 gagacattta tattgaattt tcaaaaattc ttactttttt tttggatgga cgcaaagaag 4500 tttaataatc atattacatg gcaataccac catatacata tccatatcta atcttactta 4560 tatgttgtgg aaatgtaaag agccccatta tcttagccta aaaaaacctt ctctttggaa 4620 ctttcagtaa tacgcttaac tgctcattgc tatattgaag tacggattag aagccgccga 4680 gcgggcgaca gccctccgac ggaagactct cctccgtgcg tcctggtctt caccggtcgc 4740 gttcctgaaa cgcagatgtg cctcgcgccg cactgctccg aacaataaag attctacaat 4800 actagctttt atggttatga agaggaaaaa ttggcagtaa cctggcccca caaaccttca 4860 aatcaacgaa tcaaattaac aaccatagga taataatgcg attagttttt tagccttatt 4920 tctggggtaa ttaatcagcg aagcgatgat ttttgatcta ttaacagata tataaatgca 4980 aaagctgcat aaccacttta actaatactt tcaacatttt cggtttgtat tacttcttat 5040 tcaaatgtca taaaagtatc aacaaaaaat tgttaatata cctctatact ttaacgtcaa 5100 ggagaaaaaa ctataatgac tgccgacaac aatagtatgc cccatggtgc agtatctagt 5160 tacgccaaat tagtgcaaaa ccaaacacct gaagacattt tggaagagtt tcctgaaatt 5220 attccattac aacaaagacc taatacccga tctagtgaga cgtcaaatga cgaaagcgga 5280 gaaacatgtt tttctggtca tgatgaggag caaattaagt taatgaatga aaattgtatt 5340 gttttggatt gggacgataa tgctattggt gccggtacca agaaagtttg tcatttaatg 5400 gaaaatattg aaaagggttt actacatcgt gcattctccg tctttatttt caatgaacaa 5460 ggtgaattac ttttacaaca aagagccact gaaaaaataa ctttccctga tctttggact 5520 aacacatgct gctctcatcc actatgtatt gatgacgaat taggtttgaa gggtaagcta 5580 gacgataaga ttaagggcgc tattactgcg gcggtgagaa aactagatca tgaattaggt 5640 attccagaag atgaaactaa gacaaggggt aagtttcact ttttaaacag aatccattac 5700 atggcaccaa gcaatgaacc atggggtgaa catgaaattg attacatcct attttataag 5760 atcaacgcta aagaaaactt gactgtcaac ccaaacgtca atgaagttag agacttcaaa 5820 tgggtttcac caaatgattt gaaaactatg tttgctgacc caagttacaa gtttacgcct 5880 tggtttaaga ttatttgcga gaattactta ttcaactggt gggagcaatt agatgacctt 5940 tctgaagtgg aaaatgacag gcaaattcat agaatgctat aaacaggccc cttttccttt 6000 gtcgatatca tgtaattagt tatgtcacgc ttacattcac gccctccccc cacatccgct 6060 ctaaccgaaa aggaaggagt tagacaacct gaagtctagg tccctattta tttttttata 6120 gttatgttag tattaagaac gttatttata tttcaaattt ttcttttttt tctgtacaaa 6180 cgcgtgtacg catgtaacat tatactgaaa accttgcttg agaaggtttt gggacgctcg 6240 aaggctttaa tttgcacgct gcaggtcgac aacccttaat ataggtgata tcagatccac 6300 tagtggcaat tccccattag agtcaaataa aaggatgcct cacggaggta tgttacccgc 6360 gctatttcac atggctcatt gaattagagg tggaatttgg tgtaccctcc cctcctcatc 6420 tgatgaagta gtgatccgac aattcttaaa agttgtagac attactttta ccaccaacta 6480 agttgtattt atattgctac ccttatcctt ttatatctaa ctagcgctca taaggttggg 6540 gcaatactaa aactgtgttc ttattcaact cattaaatac gtggcagtac gtaccctatt 6600 agaaacaata ggaaacagca gagtcggaag aagccaaatg ccagatttga agtccaaaac 6660 cttgtcaagc caatctttgg gagcggctat tcctccagaa attgtgtacc aaatacttac 6720 ataccagttt agggatttgt taagaaatga ccatccaggt acagcagaaa agtttaacga 6780 aaacttaact acatttgtca aaagtaattt aactgttaat aagacattct cccatatttg 6840 tcaagttctc atttatagat attgtaattt aactaccgca aagagatttc acggtctttt 6900 acagacttta aagggaaata gatgcctttg caataagatt gaagtagccg atttccaaga 6960 attaacgtct attggactgg ggaggtccag tgagatgaat aaaatgatta aaaatctaac 7020 taacgaaact cttcttgagt ttctaatgct cacaaaagca aacttaaggg aattcttagc 7080 atgtgaaaat attcaagacg atttagacga taatatcata aagtatattc tatctccaga 7140 aaaagtcttg agtgtagtgg atttctgtgg ctgttcaggt accactttca cagaaagttt 7200 tattaaggcc ctggataaat actaccctaa taaatcgatt gatcagtacc ggctggaacc 7260 tattcaacaa aattatcaga ttacctgctt aggcctaaat gattgtattg atctaccatc 7320 acacgttctt tggaagatct taaaaatgtt accagaacta caaaaacttg atttatctca 7380 tacctccatt gatggc 7396 <210> 20 <211> 6365 <212> DNA <213> Artificial Sequence <400> 20 ggccgcctca aattacacac atacagatag gtcacatcat agaggtgata atgttttggg 60 tgatgcctct ttaggcgatt cgtttaattc aatgccagat tttactccaa gaaatttctt 120 gatacctaca ttaaaaaaat cacgggaaaa gttcgagcct catctatcca atccaaacca 180 acattttgga aatgtaacct tctaataatt tgtcatttga ctgacgaatc gttaggcaca 240 gttatatagt tatatacaca tagacataca tatgttcgca tatatattat attactatgc 300 attcacctaa atagctactg ctctattaat acagtgcttt tttgatggct ttaatttaac 360 ttattagaca ttactttgcg tttattcccc tccaatatat ttttatgttt gtttttcttg 420 aacattttat gatttcttgc tcttggcggc tactgatttt gttaagcaac tcatcaagag 480 gtcaaaaaaa gtagaacgag atctttgtgt tcggttaccc ggctcagatc ctaacttggt 540 atgtttattc gtataagtta ctgttgtcca caggcaatac tctgcagaaa attaaaacgg 600 cattaatgct aggacaacca gaattgttac tactgtatgt gcgatagttg ataactgcaa 660 cattatgccc ggtatattct caaaaaaccc tattactgca tacgaagaaa tcgcaagaga 720 aatctttcgg tttggaaaag ctcactgtga ggttccttgg agccaatagt aatacagcac 780 aatccaagga aaaatctggc ctatatgcaa ggaaggagag atagtcaaaa gcattctttc 840 ccctagaagt tggtgcatat atggcatcgt taaaacatat tacccccaaa atttcttctc 900 taaacgatgt gcttggcctt tgttttggtt tttgatgtcg gtcgtttgag gccccttgcg 960 gaaaatcgag atcgccgaat ggcacgcgag ggaagggaaa taaggtttaa aggcactgaa 1020 acaataggca agaagtaggc gagagccgac atacgagagc ttcattgctc ctacatttat 1080 cggatggcgc acatagctaa aaaaggaaga ttttcactca taagggaaag gaaccgcatg 1140 actcaagaga agcatgtggt ttttgagttt ttttcgttga attttcaggt aaagctcaat 1200 agttatgaca attacaacaa cagaattctt tctatatatg cacgaacttg taatatggaa 1260 gaaattatga cgtacaaact ataaagtaaa tattttacgt aacacatggt gctgttgtgc 1320 ttctttttca agagaatacc aatgacgtat gactaagttt aggatttaat gcaggtgacg 1380 gacccatctt tcaaacgatt tatatcagtg gcgtccaaat tgttaggttt tgttggttca 1440 gcaggtttcc tgttgtgggt catatgactt tgaaccaaat ggccggctgc tagggcagca 1500 cataaggata attcacctgc caagacggca caggcaacta ttcttgctaa ttgacgtgcg 1560 ttggtaccag gagcggtagc atgcgggcct cttacaccta ataagtccaa catggcacct 1620 tgtggttcta gaacagtacc accaccgatg gtacctactt cgatggatgg catggatacg 1680 gaaattctca aatcaccgtc cacttctttc atcaatgtta tacagttgga actttcaaca 1740 ttttgtgcag gatcttgtcc taatgccaag aaaacagctg tcactaaatt agctgcatgt 1800 gcgttaaatc caccaacaga cccagccatt gcagatccaa ccaaattctt agcaatgttc 1860 aactcaacca atgcggaaac atcacttttt aacacttttc tgacaacatc accaggaata 1920 gtagcttctg cgacgacact cttaccacga ccttcgatcc agttgatggc agctggtttt 1980 ttgtcggtac agtagttacc agaaacggag acaacctcca tatcttccca gccatactct 2040 tctaccattt gctttaatga gtattcgaca cctttagaaa tcatattcat acccattgcg 2100 tcaccagtag ttgttctaaa tctcatgaag agtaaatctc ctgctagaca agtttgaata 2160 tgttgcagac gtgcaaatct tgatgtagag ttaaaagctt ttttaattgc gttttgtccc 2220 tcttctgagt ctaaccatat cttacaggca ccagatcttt tcaaagttgg gaaacggact 2280 actgggcctc ttgtcatacc atccttagtt aaaacagttg ttgcaccacc gccagcattg 2340 attgccttac agccacgcat ggcagaagct accaaacaac cctctgtagt tgccattggt 2400 atatgataag atgtaccatc gataaccaag gggcctataa caccaacggg caaaggcatg 2460 taacctataa cattttcaca acaagcgcca aatacgcggt cgtagtcata atttttatat 2520 ggtaaacgat cagatgctaa tacaggagct tctgccaaaa ttgaaagagc cttcctacgt 2580 accgcaaccg ctctcgtagt atcacctaat tttttctcca aagcgtacaa aggtaactta 2640 ccgtgaataa ccaaggcagc gacctctttg ttcttcaatt gttttgtatt tccactactt 2700 aataatgctt ctaattcttc taaaggacgt attttcttat ccaagctttc aatatcgcgg 2760 gaatcatctt cctcactaga tgatgaaggt cctgatgagc tcgattgcgc agatgataaa 2820 cttttgactt tcgatccaga aatgactgtt ttattggtta aaaccattta tattgaattt 2880 tcaaaaattc ttactttttt tttggatgga cgcaaagaag tttaataatc atattacatg 2940 gcaataccac catatacata tccatatcta atcttactta tatgttgtgg aaatgtaaag 3000 agccccatta tcttagccta aaaaaacctt ctctttggaa ctttcagtaa tacgcttaac 3060 tgctcattgc tatattgaag tacggattag aagccgccga gcgggcgaca gccctccgac 3120 ggaagactct cctccgtgcg tcctggtctt caccggtcgc gttcctgaaa cgcagatgtg 3180 cctcgcgccg cactgctccg aacaataaag attctacaat actagctttt atggttatga 3240 agaggaaaaa ttggcagtaa cctggcccca caaaccttca aatcaacgaa tcaaattaac 3300 aaccatagga taataatgcg attagttttt tagccttatt tctggggtaa ttaatcagcg 3360 aagcgatgat ttttgatcta ttaacagata tataaatgca aaagctgcat aaccacttta 3420 actaatactt tcaacatttt cggtttgtat tacttcttat tcaaatgtca taaaagtatc 3480 aacaaaaaat tgttaatata cctctatact ttaacgtcaa ggagaaaaaa ctataatgaa 3540 actctcaact aaactttgtt ggtgtggtat taaaggaaga cttaggccgc aaaagcaaca 3600 acaattacac aatacaaact tgcaaatgac tgaactaaaa aaacaaaaga ccgctgaaca 3660 aaaaaccaga cctcaaaatg tcggtattaa aggtatccaa atttacatcc caactcaatg 3720 tgtcaaccaa tctgagctag agaaatttga tggcgtttct caaggtaaat acacaattgg 3780 tctgggccaa accaacatgt cttttgtcaa tgacagagaa gatatctact cgatgtccct 3840 aactgttttg tctaagttga tcaagagtta caacatcgac accaacaaaa ttggtagatt 3900 agaagtcggt actgaaactc tgattgacaa gtccaagtct gtcaagtctg tcttgatgca 3960 attgtttggt gaaaacactg acgtcgaagg tattgacacg cttaatgcct gttacggtgg 4020 taccaacgcg ttgttcaact ctttgaactg gattgaatct aacgcatggg atggtagaga 4080 cgccattgta gtttgcggtg atattgccat ctacgataag ggtgccgcaa gaccaaccgg 4140 tggtgccggt actgttgcta tgtggatcgg tcctgatgct ccaattgtat ttgactctgt 4200 aagagcttct tacatggaac acgcctacga tttttacaag ccagatttca ccagcgaata 4260 tccttacgtc gatggtcatt tttcattaac ttgttacgtc aaggctcttg atcaagttta 4320 caagagttat tccaagaagg ctatttctaa agggttggtt agcgatcccg ctggttcgga 4380 tgctttgaac gttttgaaat atttcgacta caacgttttc catgttccaa cctgtaaatt 4440 ggtcacaaaa tcatacggta gattactata taacgatttc agagccaatc ctcaattgtt 4500 cccagaagtt gacgccgaat tagctactcg cgattatgac gaatctttaa ccgataagaa 4560 cattgaaaaa acttttgtta atgttgctaa gccattccac aaagagagag ttgcccaatc 4620 tttgattgtt ccaacaaaca caggtaacat gtacaccgca tctgtttatg ccgcctttgc 4680 atctctatta aactatgttg gatctgacga cttacaaggc aagcgtgttg gtttattttc 4740 ttacggttcc ggtttagctg catctctata ttcttgcaaa attgttggtg acgtccaaca 4800 tattatcaag gaattagata ttactaacaa attagccaag agaatcaccg aaactccaaa 4860 ggattacgaa gctgccatcg aattgagaga aaatgcccat ttgaagaaga acttcaaacc 4920 tcaaggttcc attgagcatt tgcaaagtgg tgtttactac ttgaccaaca tcgatgacaa 4980 atttagaaga tcttacgatg ttaaaaaata atcatgtaat tagttatgtc acgcttacat 5040 tcacgccctc cccccacatc cgctctaacc gaaaaggaag gagttagaca acctgaagtc 5100 taggtcccta tttatttttt tatagttatg ttagtattaa gaacgttatt tatatttcaa 5160 atttttcttt tttttctgta cagacgcgtg tacgcatgta acattatact gaaaaccttg 5220 cttgagaagg ttttgggacg ctcgaaggct ttaatttgcc cacttttcaa tgaaacggat 5280 attgatatgc tagtaaaagg acgagctcaa gagcgaaaat ataagtaaag aattcgagtg 5340 cacttgtctc catgcagcaa gatttcatat gagtcttttt tatcttttta ctttttacat 5400 tacacgatat gcactttatg aaaatttaac gaggttggaa gccggataat caaccaaaat 5460 caggcacgaa ggcacactcg tatatgcatg ttgttgaaac tctgttacgc tgaactaaca 5520 atcacacatg tagaggtcac cgggaaaagt tgcgacccca tggaaggtcg atctcttcgt 5580 ttggctttgc ttggctggcg gcattgcgct tcttcgctta tacccgtctc ttgacgctcg 5640 agctcgttca ttgagatacc tttattcttg cacattttct ggcttttttc gctactcggg 5700 tacatgtaat catgcacaca gaaggtgctg tagggtgaaa gttcctttgt gctgtcgttt 5760 gtttttaatg ccaaactttc cggtgatcaa taaccacctc tttttccttc aggaaacctt 5820 attattgttc ttggatagta ctaggaagta tataaggaac ctcgattttg gtattgcacg 5880 gctatacaca tctaagaaac tttgtataaa aggtggctac cctattcata gcttgatatc 5940 aataggccat ctcatcactt tttattgaaa aggaaaggag ggaaatatat ctgattcaaa 6000 ttacttgttt gcttctcttt aagacaaaag catagataat ttcagcgtgg aacgccggaa 6060 taagattggt accctcgtca gaaagttaca aataccgctt catcttcaaa atgacttcac 6120 cggaatcact atcttctcgt catatcaggc aaggaaggac atacacaacc acagacaagg 6180 tcatatcgcg gtcgtcgtcg tactcatcta atagttcaat atctaaagat tacggcgatc 6240 acacaccctt gtccgtcagc agtgcagctt cagagacatt accctcacct cagtatatgc 6300 cgataaggac attcaataca atgcctacag ctggcccaac gcctttacat ttatttcaaa 6360 atggc 6365 <210> 21 <211> 58 <212> DNA <213> Artificial Sequence <400> 21 gcttgagaag gttttgggac gctcgaaggc tttaatttgc ccgccatcca gtgtcgaa 58 <210> 22 <211> 59 <212> DNA <213> Artificial Sequence <400> 22 cattatacga agttatatta agggttgtcg acctgcagcc ataacgcgtt acacggaag 59 <210> 23 <211> 59 <212> DNA <213> Artificial Sequence <400> 23 cgggccctcc ttactgctct ccttccgtgt aacgcgttat ggctgcaggt cgacaaccc 59 <210> 24 <211> 59 <212> DNA <213> Artificial Sequence <400> 24 agtaaaaaag gagtagaaac attttgaagc tatgagctcg ttagtatcga atcgacagc 59 <210> 25 <211> 59 <212> DNA <213> Artificial Sequence <400> 25 ggtcgctata ctgctgtcga ttcgatacta acgagctcat agcttcaaaa tgtttctac 59 <210> 26 <211> 59 <212> DNA <213> Artificial Sequence <400> 26 aagggttctc gagagctcgt tttcgacact ggatggcggg caaattaaag ccttcgagc 59 <210> 27 <211> 38 <212> DNA <213> Artificial Sequence <400> 27 gggaacaaaa gcgcggccgc gacattgaac tcttggcc 38 <210> 28 <211> 59 <212> DNA <213> Artificial Sequence <400> 28 tccaaaaaaa aagtaagaat ttttgaaaat tcaatataac ataacgcgtt acacggaag 59 <210> 29 <211> 59 <212> DNA <213> Artificial Sequence <400> 29 ccttccgtgt aacgcgttat gttatattga attttcaaaa attcttactt tttttttgg 59 <210> 30 <211> 44 <212> DNA <213> Artificial Sequence <400> 30 gtttagttga gagtttcatt atagtttttt ctccttgacg ttaa 44 <210> 31 <211> 44 <212> DNA <213> Artificial Sequence <400> 31 caaggagaaa aaactataat gaaactctca actaaacttt gttg 44 <210> 32 <211> 47 <212> DNA <213> Artificial Sequence <400> 32 gacataacta attacatgat tattttttaa catcgtaaga tcttcta 47 <210> 33 <211> 46 <212> DNA <213> Artificial Sequence <400> 33 ttacgatgtt aaaaaataat catgtaatta gttatgtcac gcttac 46 <210> 34 <211> 59 <212> DNA <213> Artificial Sequence <400> 34 attatacgaa gttatattaa gggttgtcga cctgcagcgc aaattaaagc cttcgagcg 59 <210> 35 <211> 58 <212> DNA <213> Artificial Sequence <400> 35 gcttgagaag gttttgggac gctcgaaggc tttaatttgc gctgcaggtc gacaaccc 58 <210> 36 <211> 58 <212> DNA <213> Artificial Sequence <400> 36 taagaaaaat ttcgagagag tcgccgatag tagccactag tggatctgat atcaccta 58 <210> 37 <211> 59 <212> DNA <213> Artificial Sequence <400> 37 ctatacgaag ttattaggtg atatcagatc cactagtggc tactatcggc gactctctc 59 <210> 38 <211> 55 <212> DNA <213> Artificial Sequence <400> 38 gagacggtca cagcttgtct gtgcggccgc ctgagcagca aaataccatt tttta 55 <210> 39 <211> 46 <212> DNA <213> Artificial Sequence <400> 39 tggtattttg ctgctcaggc ggccgcacag acaagctgtg accgtc 46 <210> 40 <211> 45 <212> DNA <213> Artificial Sequence <400> 40 ccaagagttc aatgtcgcgg ccgcgctttt gttcccttta gtgag 45 <210> 41 <211> 59 <212> DNA <213> Artificial Sequence <400> 41 catgaggtcg ctcttattga ccacacctct accggttacg tggattgagc cagcaatac 59 <210> 42 <211> 24 <212> DNA <213> Artificial Sequence <400> 42 aaggtggatg ggtaggttat atag 24 <210> 43 <211> 27 <212> DNA <213> Artificial Sequence <400> 43 gtatctcttt gctatatatc tctgtgc 27 <210> 44 <211> 30 <212> DNA <213> Artificial Sequence <400> 44 caatagtaat actatggtaa tgaatgacgc 30 <210> 45 <211> 24 <212> DNA <213> Artificial Sequence <400> 45 cttcttcgta aatgtggccg ttat 24 <210> 46 <211> 23 <212> DNA <213> Artificial Sequence <400> 46 cgtacaaagg taacttaccg tga 23 <210> 47 <211> 23 <212> DNA <213> Artificial Sequence <400> 47 caattgaaga acaaagaggt cgc 23 <210> 48 <211> 59 <212> DNA <213> Artificial Sequence <400> 48 gtttaataat gatctgtatt gctggctcaa tccacgtaac cggtagaggt gtggtcaat 59 <210> 49 <211> 25 <212> DNA <213> Artificial Sequence <400> 49 gataaaggca tccccgatta tattc 25 <210> 50 <211> 19 <212> DNA <213> Artificial Sequence <400> 50 cacaaagtat gcgcaatcc 19 <210> 51 <211> 59 <212> DNA <213> Artificial Sequence <400> 51 taaatgatca tatgtctcta attttggaag ttttagagct agaaatagca agttaaaat 59 <210> 52 <211> 59 <212> DNA <213> Artificial Sequence <400> 52 tttctagctc taaaacttcc aaaattagag acatatgatc atttatcttt cactgcgga 59 <210> 53 <211> 34 <212> DNA <213> Artificial Sequence <400> 53 tcgacaaccc ttaatatagg tgatatcaga tcca 34 <210> 54 <211> 34 <212> DNA <213> Artificial Sequence <400> 54 ctagtggatc tgatatcacc tatattaagg gttg 34 <210> 55 <211> 25 <...

Claims

1. A strain for simultaneously producing sesquiterpenes and pantothenic acid, characterized in that, The strain uses Saccharomyces cerevisiae as the starting strain and contains genes related to the mevalonate pathway, genes for the synthesis of sesquiterpenes, and genes related to pantothenic acid synthesis; the sesquiterpene is β-farnesene; The genes related to the mevalonate pathway include ERG10, ERG13, tHMG1, ERG12, ERG8, MVD1, IDI1, and ERG20 genes; the copy numbers of the genes in the mevalonate pathway are ERG10:ERG13:tHMG1:ERG12:ERG8:MVD1:IDI1:ERG20 = 2:2:X:2:2:2:2:2, where X is an integer greater than or equal to 1; The β-farnesene synthesis gene is bFS, and the bFS gene contains F11S, M35T, T319S, I434T, I460V mutations, and its nucleotide sequence is as shown in SEQ ID NO.1; The ERG10, ERG13, tHMG1, ERG12, ERG8, MVD1, IDI1, and ERG20 genes are derived from Accession / GENE id 856079, 854913, 854900, NM_001182715.1, CP046093.1, NM_001183220.1, NM_001183931.1, 853272 in NCBI in sequence; The strains that simultaneously produce sesquiterpenes and pantothenic acid are JFS2 and JFS4, and the preparation steps include: Co-transforming plasmids pPT1, pPT2, and pXZ162 into Saccharomyces cerevisiae JVA139 to obtain strain JFS1; Knocking out the GAL80 gene of the strain JFS1 to obtain strain JFS2; Alternatively, co-transforming plasmids pPT1, pPT3, and pXZ162 into Saccharomyces cerevisiae JVA139 to obtain strain JFS3; Knocking out the GAL80 gene of the strain JFS3 to obtain strain JFS4; Among them, The plasmid pPT1 uses pMD19T as the backbone vector, the promoters are pGAL1, pGAL2, pGAL7, and pGAL10, and it contains ilv2, ilv6, ilv5, and ilv3 genes. The nucleotide sequence of the plasmid pPT1 is as shown in SEQ ID NO.3; The plasmid pPT2 uses pMD19T as the backbone vector, the promoters are pGAL1, pGAL2, pGAL7, and pGAL10, and it contains Ecm31p, pan5, pan6, and PAND genes. The nucleotide sequence of the plasmid pPT2 is as shown in SEQ ID NO.4; The plasmid pPT3 uses pMD19T as the backbone vector, the promoters are pGAL1, pGAL7, pGAL10, and it contains Ecm31p, pan5, and pan6 genes. The nucleotide sequence of the plasmid pPT3 is as shown in SEQ ID NO.5; The nucleotide sequence of the plasmid pXZ162 is shown as SEQ ID NO.6; the Saccharomyces cerevisiae JVA139 contains the genes related to the mevalonate pathway and the β-farnesene synthesis gene bFS.

2. Application of the strain for simultaneously producing sesquiterpenes and pantothenic acid as claimed in claim 1 in the production of β-farnesene and pantothenic acid.

3. A strain for simultaneously producing sesquiterpenes and squalene, characterized in that, The strain uses Saccharomyces cerevisiae as the starting strain and contains the genes related to the mevalonate pathway, the sesquiterpene synthesis gene, and the genes related to squalene synthesis; the sesquiterpene is β-farnesene; The genes related to the mevalonate pathway include ERG10, ERG13, tHMG1, ERG12, ERG8, MVD1, IDI1, and ERG20 genes; the copy numbers of the genes in the mevalonate pathway are ERG10:ERG13:tHMG1:ERG12:ERG8:MVD1:IDI1:ERG20 = 2:2:X:2:2:2:2:2, where X is an integer greater than or equal to 1; The β-farnesene synthesis gene is bFS, and the bFS gene contains mutations of F11S, M35T, T319S, I434T, and I460V, and its nucleotide sequence is shown as SEQ ID NO.1; The ERG10, ERG13, tHMG1, ERG12, ERG8, MVD1, IDI1, and ERG20 genes are derived from Accession / GENE id 856079, 854913, 854900, NM_001182715.1, CP046093.1, NM_001183220.1, NM_001183931.1, 853272 in NCBI in sequence; The strain for simultaneously producing sesquiterpenes and squalene is JSX2 or JSX3, and the preparation steps include: The strain JSX1 obtained by co-transforming the linearized plasmid pXZ171, pXZ159, and pXZ161 into Saccharomyces cerevisiae JVA139; then the strain JSX2 obtained by co-transforming the linearized plasmid pSX01 and pXZ162 into the strain JSX1; Or the strain JSX3 obtained by knocking out the GAL80 gene of the strain JSX2; Wherein, The plasmid pXZ171 uses pMD19T as the backbone vector, the promoters are pGAL1, pGAL7, and pGAL10, and it contains the ADH2, ACS2, and ALD6 genes. The nucleotide sequence of the plasmid pXZ171 is shown as SEQ ID NO.7; The plasmid pXZ159 uses pMD19T as the backbone vector, the promoter is pGAL1, and it contains the POS5 gene. The nucleotide sequence of the plasmid pXZ159 is shown as SEQ ID NO.8; The plasmid pSX01 uses pMD19T as the backbone vector, the promoter is pHXT1, and it contains the ERG9 gene. The nucleotide sequence of the plasmid pSX01 is shown as SEQ ID NO.9; The nucleotide sequence of the plasmid pXZ161 is shown as SEQ ID NO.10; The Saccharomyces cerevisiae JVA139 contains the relevant genes of the mevalonate pathway and the β-farnesene synthesis gene bFS.

4. Use of the strain for simultaneously producing sesquiterpenes and squalene as claimed in claim 3 in the production of β-farnesene and squalene.

5. A strain for simultaneously producing sesquiterpenes and fatty acids, characterized in that, The strain uses Saccharomyces cerevisiae as the starting strain and contains the relevant genes of the mevalonate pathway, the sesquiterpene synthesis gene, and the relevant genes of fatty acid synthesis; the sesquiterpene is β-farnesene; The relevant genes of the mevalonate pathway include the ERG10, ERG13, tHMG1, ERG12, ERG8, MVD1, IDI1, and ERG20 genes; the copy numbers of the genes in the mevalonate pathway are ERG10:ERG13:tHMG1:ERG12:ERG8:MVD1:IDI1:ERG20 = 2:2:X:2:2:2:2:2, where X is an integer greater than or equal to 1; The β-farnesene synthesis gene is bFS, and the bFS gene contains the F11S, M35T, T319S, I434T, I460V mutations, and its nucleotide sequence is as shown in SEQ ID NO.1; The ERG10, ERG13, tHMG1, ERG12, ERG8, MVD1, IDI1, and ERG20 genes are derived from Accession / GENE id 856079, 854913, 854900, NM_001182715.1, CP046093.1, NM_001183220.1, NM_001183931.1, 853272 in NCBI in sequence; The strain for simultaneously producing sesquiterpenes and fatty acids is JZF01 or JZF02, and the preparation steps include: The strain JZF01 obtained by co-transforming the linearized plasmids pZF01, pZF02, and pZF03 and pZF04 into Saccharomyces cerevisiae JVA139; Or the strain JZF02 obtained by knocking out the GAL80 gene of the strain JZF01; Wherein, The plasmid pZF01 uses pMD19T as the backbone vector, the promoter is pHXT1, and it contains the tesA gene. The nucleotide sequence of the plasmid pZF01 is as shown in SEQ ID NO.11; The plasmid pZF02 uses pMD19T as the backbone vector, the promoter is pHXT1, and it contains the ACC1 gene. The nucleotide sequence of the plasmid pZF02 is as shown in SEQ ID NO.12; The plasmid pZF03 uses pMD19T as the backbone vector, the promoter is pHXT1, and it contains the FAS1 and FAS2 genes. The nucleotide sequence of the plasmid pZF03 is as shown in SEQ ID NO.13; The nucleotide sequence of the plasmid pZF04 is as shown in SEQ ID NO.14; the Saccharomyces cerevisiae JVA139 contains the relevant genes of the mevalonate pathway and the β-farnesene synthesis gene bFS.

6. Use of the strain for simultaneously producing sesquiterpenes and fatty acids as claimed in claim 4 in the production of β-farnesene and fatty acids.

7. A strain for simultaneously producing sesquiterpenes and vitamin B2, characterized in that, The strain uses Saccharomyces cerevisiae as the starting strain and contains genes related to the mevalonate pathway, genes for the synthesis of sesquiterpenes, and genes related to the synthesis of vitamin B2; the sesquiterpene is β-farnesene; The genes related to the mevalonate pathway include ERG10, ERG13, tHMG1, ERG12, ERG8, MVD1, IDI1, and ERG20 genes; the copy numbers of the genes in the mevalonate pathway are ERG10: ERG13: tHMG1: ERG12: ERG8: MVD1: IDI1: ERG20 = 2: 2: X: 2: 2: 2: 2: 2, where X is an integer greater than or equal to 1; The β-farnesene synthesis gene is bFS, and the bFS gene contains mutations of F11S, M35T, T319S, I434T, and I460V, and its nucleotide sequence is as shown in SEQ ID NO.1; The ERG10, ERG13, tHMG1, ERG12, ERG8, MVD1, IDI1, and ERG20 genes are derived from Accession / GENE id 856079, 854913, 854900, NM_001182715.1, CP046093.1, NM_001183220.1, NM_001183931.1, 853272 in NCBI in sequence; The strain that simultaneously produces sesquiterpenes and vitamin B2 is JVB01 or JVB02, and the preparation steps include: The strain JVB01 obtained by co-transforming the linearized plasmid pVB1 and pVB2 and pXZ162 into Saccharomyces cerevisiae JVA139; Or the strain JVB02 obtained by knocking out the GAL80 gene of the strain JVB01; Among them, The plasmid pVB1 uses pMD19T as the backbone vector, the promoters are pGAL1, pGAL7, and pGAL10, and it contains RIB1, RIB7, and RIB2 genes, and the nucleotide sequence of the plasmid pVB1 is as shown in SEQ ID NO.15; The plasmid pVB2 uses pMD19T as the backbone vector, the promoters are pGAL1, pGAL7, and pGAL10, and it contains RIB3, RIB4, and RIB5 genes, and the nucleotide sequence of the plasmid pVB2 is as shown in SEQ ID NO.16; The nucleotide sequence of the plasmid pXZ162 is as shown in SEQ ID NO.6; the Saccharomyces cerevisiae JVA139 contains the genes related to the mevalonate pathway and the β-farnesene synthesis gene bFS.

8. Use of the strain that simultaneously produces sesquiterpenes and vitamin B2 according to claim 7 in the production of β-farnesene and vitamin B2.

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