Polypeptides, underwater adhesives, adhesive coatings, and methods of making and using the same
By using a polypeptide with the amino acid sequence Lys-DOPA-Phe-Lys-DOPA-Phe to form an underwater adhesive, the problems of difficult and expensive synthesis in the prior art are solved, and a highly efficient adhesive coating with good underwater adhesion and environmental adaptability is achieved on a variety of substrate surfaces.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- 天津大学浙江研究院
- Filing Date
- 2022-09-30
- Publication Date
- 2026-04-28
AI Technical Summary
Existing protein-based underwater adhesives are difficult to synthesize, expensive, and have complex amino acid sequences, which limits their widespread application.
A peptide with the amino acid sequence Lys-DOPA-Phe-Lys-DOPA-Phe was used as an underwater adhesive. The adhesive solution was formed by mixing it with a solvent, and the substrate was immersed in the solution and then cleaned to form an adhesive coating. The interaction between the peptides was used to improve the adhesion.
A simple synthetic peptide underwater adhesive was developed to form considerable underwater adhesion on a variety of substrate surfaces, exhibiting good environmental adaptability, especially demonstrating excellent adhesion performance in acidic and saline environments.
Smart Images
Figure CN115819501B_ABST
Abstract
Description
Technical Field
[0001] This invention relates to the field of biomaterials technology, and in particular to a polypeptide, an underwater adhesive, an adhesive coating, and their preparation methods and applications. Background Technology
[0002] Underwater adhesives have wide applications in daily life, industrial production, and biomedicine. In industrial production, underwater adhesives are commonly used in the construction and maintenance of underwater facilities, the repair of water pipelines, and shipbuilding. In the biomedical field, underwater adhesives are indispensable for wound treatment, tissue repair, and bone fixation. Among them, protein-based underwater adhesives have advantages such as rapid and efficient adhesion, high bonding strength, and good biocompatibility (non-toxic and harmless), making them widely used in many fields, especially in biomedicine.
[0003] In the design and development of protein-based underwater adhesives, biomimetic underwater adhesion proteins and peptides inspired by the adhesion proteins of marine organisms such as mussels, barnacles, and sandworms account for a large proportion. Common biomimetic protein-based adhesive materials contain the dopa (DOPA) structure unique to mussel and sandworm adhesion proteins and introduce other amino acid groups to enhance the performance of protein-based underwater adhesive materials. Previous reports have investigated the mechanism by which lysine enhances the adhesion performance of protein-based underwater adhesive materials, but there are few reports on the introduction and research of other amino acids. Furthermore, the design of these underwater adhesion protein materials is mainly based on the sequences of marine organism adhesion proteins, which typically contain dozens or even hundreds of amino acid sequences. Their complex structures make their synthesis difficult and expensive, limiting their applications. Summary of the Invention
[0004] The purpose of this invention is to provide a polypeptide and an underwater adhesive that has the advantages of simple sequence, convenient synthesis, and considerable underwater adhesion.
[0005] The present invention also aims to provide an adhesive coating, its preparation method, and its application to polypeptides.
[0006] To achieve the above objectives, embodiments of the present invention provide a polypeptide with the amino acid sequence Lys-DOPA-Phe-Lys-DOPA-Phe.
[0007] Embodiments of the present invention also provide an application of a polypeptide in an underwater adhesive, wherein the amino acid sequence of the polypeptide is Lys-DOPA-Phe-Lys-DOPA-Phe.
[0008] Embodiments of the present invention also provide an underwater adhesive comprising a polypeptide having the amino acid sequence Lys-DOPA-Phe-Lys-DOPA-Phe.
[0009] Embodiments of the present invention also provide an adhesive coating, the adhesive coating being prepared from an underwater adhesive comprising a polypeptide having the amino acid sequence Lys-DOPA-Phe-Lys-DOPA-Phe.
[0010] Embodiments of the present invention also provide a method for preparing the adhesive coating as described above, comprising the following steps:
[0011] S1. Mix the polypeptide with a solvent to obtain an adhesive solution;
[0012] S2. Immerse the substrate in the adhesive solution;
[0013] S3. After separation, the adhesive coating attached to the substrate is obtained.
[0014] In one or more embodiments of the present invention, the solvent is dimethyl sulfoxide.
[0015] In one or more embodiments of the present invention, the modified concentration of the polypeptide in the adhesive solution is 5-100 μg / mL.
[0016] In one or more embodiments of the present invention, in step S2, the substrate is immersed in the adhesive solution for a time greater than 0.5 h.
[0017] In one or more embodiments of the present invention, the substrate includes one of mica, gold, glass, and plastic.
[0018] In one or more embodiments of the present invention, step S3 includes the separation step:
[0019] The substrate was removed and washed with ultrapure water to remove excess peptides.
[0020] Compared with the prior art, the polypeptide and underwater adhesive of the present invention uses a polypeptide synthesized from six amino acids as raw materials as an underwater adhesive. The polypeptide has the advantages of simple sequence, convenient synthesis, and considerable underwater adhesion. Moreover, the underwater adhesive of the present invention can form an adhesive coating on the surface of various materials, and has broad application prospects. Attached Figure Description
[0021] Figure 1 This is a flowchart illustrating the preparation of an adhesive coating according to an embodiment of the present invention;
[0022] Figure 2 This is a schematic diagram of a test using a surface force meter (SFA) for an adhesive coating according to an embodiment of the present invention;
[0023] Figure 3 This is a graph showing test data on the adhesion force of an adhesive coating according to an embodiment of the present invention;
[0024] Figure 4 This is a graph showing test data of the adhesion of an adhesive coating according to an embodiment of the present invention at different peptide modification concentrations;
[0025] Figure 5 This is a graph showing test data on the adhesion of an adhesive coating according to an embodiment of the present invention under different environments;
[0026] Figure 6 This is the structural formula of a polypeptide according to an embodiment of the present invention. Detailed Implementation
[0027] The specific embodiments of the present invention will now be described in detail with reference to the accompanying drawings, but it should be understood that the scope of protection of the present invention is not limited to the specific embodiments.
[0028] Unless otherwise expressly stated, throughout the specification and claims, the term "comprising" or its variations such as "including" or "comprises" shall be understood to include the stated elements or components without excluding other elements or other components.
[0029] like Figure 6 As shown, the polypeptide according to a preferred embodiment of the present invention has the amino acid sequence Lys-DOPA-Phe-Lys-DOPA-Phe.
[0030] In the present invention, Lys represents lysine, DOPA represents dopa, and Phe represents phenylalanine. The amino acids are linked together by peptide bonds, which are chemical bonds formed by the dehydration condensation of amino and carboxyl groups between amino acid molecules.
[0031] In one specific embodiment, the polypeptide of the present invention can be prepared by solid-phase synthesis.
[0032] Embodiments of the present invention also provide an underwater adhesive comprising a polypeptide with the amino acid sequence Lys-DOPA-Phe-Lys-DOPA-Phe. It is understood that the underwater adhesive of the present invention may comprise only the polypeptide with the specified sequence described above; the underwater adhesive of the present invention may also comprise a mixture of the polypeptide with the specified sequence described above and other substances.
[0033] Embodiments of the present invention also provide an adhesive coating, the adhesive coating being prepared from an underwater adhesive comprising a polypeptide having the amino acid sequence Lys-DOPA-Phe-Lys-DOPA-Phe.
[0034] After the underwater adhesive of the present invention is prepared into an adhesive coating, various functional groups existing between the polypeptides can interact. Specifically, the DOPA group and the hydroxyl group at the C-terminus of the polypeptide can form hydrogen bond interactions with the amino group of the lysine side chain, the polypeptide backbone, and other carboxyl groups; cation-π bond interactions can form between the electron-rich π system (the benzene ring of phenylalanine) and the cation (the positively charged group of the lysine side chain); π-π interactions can form between electron-rich π systems (e.g., the benzene ring of phenylalanine); simultaneously, various interactions can occur between the underwater adhesive and the substrate, including electrostatic interactions, cation-π interactions, π-π interactions, hydrophobic interactions, and hydrogen bond interactions. Through the above interactions, the adhesive coating of the present invention can exhibit considerable underwater adhesion in various environments.
[0035] like Figure 1 As shown, embodiments of the present invention also provide a method for preparing the adhesive coating as described above, comprising the following steps:
[0036] S1. Mix the polypeptide with a solvent to obtain an adhesive solution.
[0037] Specifically, the solvent is dimethyl sulfoxide. The substrate includes one of mica, gold, glass, and plastic.
[0038] Specifically, the peptide modification concentration in the adhesive solution is 5-100 μg / mL. When the peptide modification concentration is less than 50 μg / mL, the peptide cannot form a complete coating on the surface. When the peptide concentration is higher than 100 μg / mL, problems such as excessively thick adhesive coating and the formation of aggregated particles occur.
[0039] It is understandable that the peptide modification concentration refers to the peptide concentration in the adhesive solution during the process of modifying the substrate with peptides as raw materials for adhesive coatings.
[0040] Preferably, the peptide modification concentration is 50 μg / mL. Experiments have shown that when the peptide modification concentration is 50 μg / mL, the adhesive coating formed on the substrate can completely cover the surface of the substrate (here, the surface can be considered as the side of the substrate to which the adhesive coating needs to be attached). When the peptide modification concentration is less than 50 μg / mL, the adhesive coating formed on the substrate will not completely cover the surface of the substrate; for example, some pores may form within the adhesive coating, exposing part of the substrate surface to the environment. When the peptide modification concentration is greater than 50 μg / mL, although the adhesive coating formed on the substrate can completely cover the surface of the substrate, more peptide is required to maintain a peptide modification concentration greater than 50 μg / mL in the adhesive solution, resulting in increased costs.
[0041] S2. Immerse the substrate in the adhesive solution.
[0042] In one specific embodiment, in step S2, the substrate is immersed in the adhesive solution for more than 0.5 hours. Preferably, the immersion time is 1 hour. When the immersion time reaches 1 hour, the adhesive coating can be completely adhered to the surface of the substrate. If the immersion time is less than 0.5 hours, the adhesive coating may not be completely formed on the substrate, resulting in poor adhesion of the adhesive coating attached to the substrate. If the immersion time is more than 1 hour, it wastes time and increases time costs. It is understood that the immersion time of the substrate in the adhesive solution is inversely proportional to the modification concentration of the peptide in the adhesive solution.
[0043] S3. After separation, an adhesive coating is obtained that adheres to the substrate.
[0044] In step S3, the separation steps include: removing the substrate and washing it with ultrapure water to remove excess adhering short peptides.
[0045] Embodiments of the present invention also provide an application of a polypeptide in an underwater adhesive, wherein the amino acid sequence of the polypeptide is Lys-DOPA-Phe-Lys-DOPA-Phe.
[0046] The present invention’s polypeptides, underwater adhesives, adhesive coatings, their preparation methods and applications will be described below with reference to specific embodiments.
[0047] Example 1
[0048] The force-distance curve of the adhesive coating on the mica substrate surface was measured using a surface force analyzer (SFA) to obtain the underwater adhesion force of the adhesive coating. A schematic diagram of the test is shown below. Figure 2 As shown, the specific testing process is as follows.
[0049] 1) Reference spectral calibration
[0050] Before the experiment, the prepared mica substrates to be modified were placed in a cross shape on the upper and lower bases of the SFA chamber. Coarse and fine adjustments were made using a manual differential micrometer to ensure contact between the surfaces of the two mica substrates. Then, the isochromatic interference fringes (FECO) and the yellow-green light spectrum contained in the mercury lamp were obtained using a spectrometer and a high-speed camera for pre-experiment calibration.
[0051] 2) Construction of adhesive coating
[0052] a) Prepare adhesive solutions with different solvents but a peptide modification concentration of 50 μg / mL. The selected solvents are water, 5% DMSO, DMSO, 5 mM HCl, and 5 mM HAc. Here, 5% DMSO, 5 mM HCl, and 5 mM HAc refer to the concentration in water, meaning they can be considered as mixtures of DMSO, HCl, and HAc with water, respectively. The concentrations mentioned above are volume concentrations; for example, 5% DMSO means a volume ratio of DMSO to water of 5:95.
[0053] b) Immerse both mica substrates in the adhesive solution in a) for 1 hour to modify the surface and construct the adhesive coating.
[0054] c) After the adhesive coating is constructed, remove the two mica substrates and rinse the surfaces of the two mica substrates with pure water. Finally, dry the surfaces with N2, so that an adhesive coating is formed on the surfaces of both mica substrates.
[0055] 3) System equilibrium
[0056] The two mica substrates with adhesive coatings were remounted on the base of the SFA chamber in their previous positions. The buffer solution was then rapidly injected between the mica surfaces, and the positions of the two mica surfaces were adjusted to ensure the buffer solution covered both surfaces. A certain amount of water was injected into the SFA chamber to inhibit buffer evaporation, and the system was allowed to reach equilibrium for 0.5 hours.
[0057] 4) Adhesion test of adhesive coating
[0058] After the system reaches equilibrium, the distance between the two mica surfaces is adjusted to approximately 300-400 nm (at which point the force between the two surfaces is approximately 0 N). Then, a motor controls the lower surface to approach the upper surface at a constant speed of approximately 1.5 nm / s. As the two surfaces continuously approach each other, they continue to compress upon contact. Maximum compression is reached when the FECO stripes stop moving. After maintaining this state for 3 minutes, the lower surface is controlled to move in the opposite direction, separating the two surfaces. During the movement of the FECO stripes, a high-speed camera records their motion. Using the stripe displacement and other instrumental information, the force-distance curve of the adhesive coating on the two mica substrate surfaces can be calculated. When a large adhesive force exists, the adhesive coating on the two mica substrate surfaces will jump out during separation. The adhesive force (F0) of the adhesive coating can be calculated based on the jump distance and the elastic coefficient of the spring connecting the lower surface. ad The adhesion energy E per unit area between two surfaces. ad By F ad / R=1.5πE ad Define a glass cylinder, where R is the radius of curvature of the glass cylinder. A glass cylinder refers to... Figure 2In the middle, there is a structure connected to the mica substrate below.
[0059] The adhesion of the obtained adhesive coating is as follows Figure 3 As shown, the underwater adhesion of the adhesive coating (with different solvents) is as follows: 1.35 ± 0.58 mJ / m -2 0.61±0.03mJ m -2 10.34±0.31mJ m -2 0.35±0.12mJ m -2 and 1.28±0.42mJ m -2 From the above data, it can be seen that when the adhesive solution contains DMSO, the underwater adhesion of the adhesive coating can reach 10.34 ± 0.31 mJ / m². -2 .
[0060] Example 2
[0061] The force-distance curve of the adhesive coating on the mica substrate surface was measured using a surface force analyzer (SFA) to obtain the underwater adhesion force of the adhesive coating. A schematic diagram of the test is shown below. Figure 2 As shown, the specific testing process is as follows.
[0062] 1) Reference spectral calibration
[0063] Before the experiment, the prepared mica substrates to be modified were placed in a cross shape on the upper and lower bases of the SFA chamber. Coarse and fine adjustments were made using a manual differential micrometer to ensure contact between the surfaces of the two mica substrates. Then, the isochromatic interference fringes (FECO) and the yellow-green light spectrum contained in the mercury lamp were obtained using a spectrometer and a high-speed camera for pre-experiment calibration.
[0064] 2) Construction of adhesive coating
[0065] a) Prepare adhesive solutions with peptide modification concentrations of 5 μg / mL, 10 μg / mL, 50 μg / mL and 100 μg / mL respectively, using DMSO as the solvent.
[0066] b) Immerse both mica substrates in the adhesive solution in a) for 1 hour to modify the surface and construct the adhesive coating.
[0067] c) After the adhesive coating is constructed, remove the two mica substrates and rinse the surfaces of the two mica substrates with pure water. Finally, dry the surfaces with N2, so that an adhesive coating is formed on the surfaces of both mica substrates.
[0068] 3) System equilibrium
[0069] The two mica substrates with adhesive coatings were remounted on the base of the SFA chamber in their previous positions. The buffer solution was then rapidly injected between the mica surfaces, and the positions of the two mica surfaces were adjusted to ensure the buffer solution covered both surfaces. A certain amount of water was injected into the SFA chamber to inhibit buffer evaporation, and the system was allowed to reach equilibrium for 0.5 hours.
[0070] 4) Adhesion test of adhesive coating
[0071] After the system reaches equilibrium, the distance between the two mica surfaces is adjusted to approximately 300-400 nm (at which point the force between the two surfaces is approximately 0 N). Then, a motor controls the lower surface to approach the upper surface at a constant speed of approximately 1.5 nm / s. As the two surfaces continuously approach each other, they continue to compress upon contact. Maximum compression is reached when the FECO stripes stop moving. After maintaining this state for 3 minutes, the lower surface is controlled to move in the opposite direction, separating the two surfaces. During the movement of the FECO stripes, a high-speed camera records their motion. Using the stripe displacement and other instrumental information, the force-distance curve of the adhesive coating on the two mica substrate surfaces can be calculated. When a large adhesive force exists, the adhesive coating on the two mica substrate surfaces will jump out during separation. The adhesive force (F0) of the adhesive coating can be calculated based on the jump distance and the elastic coefficient of the spring connecting the lower surface. ad The adhesion energy E per unit area between two surfaces ad By F ad / R=1.5πE ad Define a glass cylinder, where R is the radius of curvature of the glass cylinder. A glass cylinder refers to... Figure 2 In the middle, there is a structure connected to the mica substrate below.
[0072] The adhesion strength of the obtained adhesive coating is shown in the attached figure. Figure 4 As shown, the underwater adhesion of the adhesive coating (with different peptide modification concentrations) is as follows: 3.97 ± 1.40 mJ / m². -2 7.40±2.56mJ m -2 10.34±0.31mJ m -2 and 9.82±1.33mJm -2 According to the examples, when the peptide modification concentration is greater than 50 μg / mL, the underwater adhesion of the adhesive coating does not increase. Therefore, it can be considered that when the peptide modification concentration is greater than 50 μg / mL, the adhesive coating has a reasonable underwater adhesion.
[0073] Example 3
[0074] The force-distance curve of the adhesive coating on the mica substrate surface was measured using a surface force analyzer (SFA) to obtain the underwater adhesion force of the adhesive coating. A schematic diagram of the test is shown below. Figure 2As shown. The specific testing process is as follows.
[0075] 1) Reference spectral calibration
[0076] Before the experiment, the prepared mica substrates to be modified were placed in a cross shape on the upper and lower bases of the SFA chamber. Coarse and fine adjustments were made using a manual differential micrometer to ensure contact between the surfaces of the two mica substrates. Then, the isochromatic interference fringes (FECO) and the yellow-green light spectrum contained in the mercury lamp were obtained using a spectrometer and a high-speed camera for pre-experiment calibration.
[0077] 2) Construction of adhesive coating
[0078] a) Prepare an adhesive solution with a peptide modification concentration of 50 μg / mL, wherein the solvent is DMSO.
[0079] b) Immerse two mica substrates in the adhesive solution in a) for 1 hour to perform surface modification and construct an adhesive coating.
[0080] c) After the adhesive coating is constructed, remove the two mica substrates and rinse the surface of the mica substrates with DMSO, 50% DMSO solution and pure water. Finally, blow the surface dry with N2, so that an adhesive coating is formed on the surface of both mica substrates.
[0081] 3) System equilibrium
[0082] The two mica substrates with adhesive coatings were remounted on the base of the SFA chamber in their previous positions. Then, 100 mM HAc buffer solution was rapidly injected between the surfaces of the two mica substrates, and the positions of the substrate surfaces were adjusted to ensure the buffer solution covered both surfaces. A certain amount of water was injected into the SFA chamber to inhibit buffer evaporation, and the system was allowed to reach equilibrium for 0.5 hours.
[0083] 4) Adhesion test of adhesive coating
[0084] After the system reached equilibrium, the distance between the two mica substrate surfaces was adjusted to approximately 300-400 nm (at which point the force between the two surfaces was approximately 0 N). Then, a motor was used to control the lower surface to approach the upper surface at a constant speed of approximately 1.5 nm / s. As the two surfaces drew closer, they continued to compress upon contact. Maximum compression was reached after the FECO stripes stopped moving. After 3 minutes, the lower surface was controlled to move in the opposite direction, separating the two surfaces. During the movement of the FECO stripes, a high-speed camera recorded their motion. Using the stripe displacement and other instrumental information, the force-distance curve of the adhesive coating on the mica substrate surface could be calculated. When a large adhesive force exists, the adhesive coating on the two mica substrate surfaces will jump out during separation. The adhesive force (F0) of the adhesive coating can be calculated based on the jump distance and the elastic coefficient of the spring connecting the lower surface. ad The adhesion energy E per unit area between two surfaces. ad By F ad / R=1.5πE ad Define R as the radius of curvature of the glass column.
[0085] The adhesion strength of the obtained adhesive coating is as follows Figure 5 As shown, the underwater adhesion of the adhesive coating in an acidic environment (100 mM HAc) can reach 7.61 ± 0.74 mJ / m². -2 .
[0086] Example 4
[0087] The force-distance curve of the adhesive coating on the mica substrate surface was measured using a surface force analyzer (SFA) to obtain the underwater adhesion force of the adhesive coating. A schematic diagram of the test is shown below. Figure 2 As shown. The specific testing process is as follows.
[0088] 1) Reference spectral calibration
[0089] Before the experiment, the prepared mica substrates to be modified were placed in a cross shape on the upper and lower bases of the SFA chamber. Coarse and fine adjustments were made using a manual differential micrometer to ensure contact between the surfaces of the two mica substrates. Then, the isochromatic interference fringes (FECO) and the yellow-green light spectrum contained in the mercury lamp were obtained using a spectrometer and a high-speed camera for pre-experiment calibration.
[0090] 2) Construction of adhesive coating
[0091] a) Prepare an adhesive solution with a peptide modification concentration of 50 μg / mL, wherein the solvent is DMSO.
[0092] b) Immerse two mica substrates in the adhesive solution in a) for 1 hour to perform surface modification and construct an adhesive coating.
[0093] c) After the adhesive coating is constructed, remove the two mica substrates and rinse the surface of the mica substrates with DMSO, 50% DMSO solution and pure water. Finally, blow the surface dry with N2, so that an adhesive coating is formed on the surface of both mica substrates.
[0094] 3) System equilibrium
[0095] Two mica substrates with adhesive coatings were remounted on the base of the SFA chamber in their previous positions. Then, 250 mM KCl buffer solution was rapidly injected between the surfaces of the two mica substrates, and the positions of the substrate surfaces were adjusted to ensure the buffer solution covered both surfaces. A certain amount of water was injected into the SFA chamber to inhibit buffer evaporation, and the system was allowed to reach equilibrium for 0.5 hours.
[0096] 4) Coating adhesion test
[0097] After the system reached equilibrium, the distance between the two mica substrate surfaces was adjusted to approximately 300-400 nm (at which point the force between the two surfaces was approximately 0 N). Then, a motor was used to control the lower surface to approach the upper surface at a constant speed of approximately 1.5 nm / s. As the two surfaces drew closer, they continued to compress upon contact. Maximum compression was reached after the FECO stripes stopped moving. After 3 minutes, the lower surface was controlled to move in the opposite direction, separating the two surfaces. During the movement of the FECO stripes, a high-speed camera recorded their motion. Using the stripe displacement and other instrumental information, the force-distance curve of the adhesive coating on the mica substrate surface could be calculated. When a large adhesive force exists, the adhesive coating on the two mica substrate surfaces will jump out during separation. The adhesive force (F0) of the adhesive coating can be calculated based on the jump distance and the elastic coefficient of the spring connecting the lower surface. ad The adhesion energy E per unit area between two surfaces. ad By F ad / R=1.5πE ad Define R as the radius of curvature of the glass column.
[0098] The adhesion strength of the obtained adhesive coating is as follows Figure 5 As shown, the underwater adhesion of the adhesive coating in a high-salt environment (250 mM KCl) can reach 3.74 ± 0.46 mJ / m². -2 .
[0099] As can be seen from the data in Example 1, when DMSO is used as the solvent of the adhesive solution, the resulting adhesive coating has good underwater adhesion.
[0100] The data from Example 2 show that when the peptide modification concentration is greater than 25 μg / mL, the resulting adhesive coating exhibits good underwater adhesion. When the peptide modification concentration is greater than 50 μg / mL, the underwater adhesion of the resulting adhesive coating is even better.
[0101] As can be seen from the data in Examples 3 and 4, the adhesive coating of the present invention has a certain degree of tolerance to acidic and salty environments.
[0102] In summary, the beneficial effects of the present invention are as follows:
[0103] 1) The underwater adhesive and the adhesive coating formed by using the peptides of the present invention have considerable underwater adhesion and a certain degree of tolerance to acidic and saline environments.
[0104] 2) The raw material polypeptide of the underwater adhesive of the present invention contains only 6 natural amino acids. Compared with natural marine organism adhesion proteins containing dozens or hundreds of amino acids, the polypeptide and underwater adhesive of the present invention have simple sequences and are simple and economical to synthesize.
[0105] 3) The underwater adhesive of the present invention can spontaneously form an adhesive coating on various substrate surfaces. The modification method is simple and efficient, and it has good adaptability to various solvents, which is beneficial to subsequent practical applications.
[0106] The foregoing description of specific exemplary embodiments of the invention is for illustrative and explanatory purposes. These descriptions are not intended to limit the invention to the precise forms disclosed, and it will be apparent that many changes and variations can be made in accordance with the foregoing teachings. The exemplary embodiments were chosen and described in order to explain the specific principles of the invention and its practical application, thereby enabling those skilled in the art to implement and utilize various different exemplary embodiments of the invention, as well as various different choices and variations. The scope of the invention is intended to be defined by the claims and their equivalents.
Claims
1. A polypeptide, characterized in that, Its amino acid sequence is Lys-DOPA-Phe-Lys-DOPA-Phe.
2. The application of a polypeptide in the preparation of underwater adhesives, characterized in that, The amino acid sequence of the polypeptide is Lys-DOPA-Phe-Lys-DOPA-Phe.
3. An underwater adhesive, characterized in that, The underwater adhesive comprises a polypeptide with the amino acid sequence Lys-DOPA-Phe-Lys-DOPA-Phe.
4. An adhesive coating, characterized in that, The adhesive coating is made from an underwater adhesive, which includes a polypeptide with the amino acid sequence Lys-DOPA-Phe-Lys-DOPA-Phe.
5. A method for preparing an adhesive coating as described in claim 4, characterized in that, Includes the following steps: S1. Mix the polypeptide with a solvent to obtain an adhesive solution; S2. Immerse the substrate in the adhesive solution; S3. After separation, the adhesive coating attached to the substrate is obtained; The solvent is dimethyl sulfoxide; the substrate includes one of mica, gold, glass, and plastic. In the adhesive solution, the modified concentration of the polypeptide is 5-100 μg / mL.
6. The method for preparing the adhesive coating as described in claim 5, characterized in that, In step S2, the substrate is immersed in the adhesive solution for a time greater than 0.5 hours.
7. The method for preparing the adhesive coating as described in claim 5, characterized in that, In step S3, the separation step includes: The substrate was removed and washed with ultrapure water to remove excess peptides.