Circulating magnetic field dynamic magnetophoresis separation device and method

Abstract

The present invention provides a circulating magnetic field dynamic magnetophoresis separation device and method, wherein the device places a linear microtube separation channel on the upper part of a linear base to form an open structure, and the linear circulating magnetic field causes magnetic particles to move in a straight line as a whole in the linear separation channel to complete the dynamic magnetophoresis separation of the magnetic particles. The external magnetic field source is optimized, and an open structure is adopted to allow dynamic magnetophoresis of magnetic particles in the linear separation channel. It is beneficial to place a test instrument around the separation channel to observe and monitor the real-time separation of magnetic particles in the separation channel. Compared with a spiral separation channel, the linear separation channel is easy to process and fix. In the circulating magnetic field dynamic magnetophoresis separation method proposed on the basis of the device, after the magnetic particles flow into the separation channel, the carrier liquid is controlled not to flow, and the magnetic particles with different characteristics are separated by magnetophoresis under the traction of the magnetic field force of the circulating magnetic field, thereby reducing the influence of the carrier liquid flow rate on the magnetophoresis separation.

Description

Technical Field

[0001] The invention relates to the technical field of positive magnetophoresis separation and analysis of magnetic particles and magnetically labeled biomolecules, and negative magnetophoresis separation of non-magnetic particles, and in particular to a circulating magnetic field dynamic magnetophoresis separation device and method. Background Art

[0002] The magnetophoretic separation of magnetic particles, magnetically labeled particles and biomolecules is used in analytical chemistry, environmental testing, bioengineering and clinical medicine to analyze the composition of substances, determine the content of certain components, manipulate genetic materials and perform sample quality control. In addition, when the carrier fluid surrounding the non-magnetic particles is a magnetic fluid, the negative magnetophoretic method can also be used to separate non-magnetic particles. Magnetophoretic separation can not only separate magnetic particles, but also non-magnetic particles, and is widely used in particle separation.

[0003] In the dynamic magnetophoretic separation of the rotating magnetic field of the three-phase winding, as in the invention patent 202111498900.X "Dynamic magnetophoretic separation device and method of magnetic particles based on rotating magnetic field" previously applied by the inventor, a cylindrical base with a hollow structure is provided with a three-phase winding with an oblique lower wire slot on the base. After the three-phase sinusoidal alternating current is connected, a rotating magnetic field will be generated in the cylindrical space of the hollow structure formed by the three-phase winding and the base, and the microtube separation channel is made into a spiral shape and placed in the cylindrical space. The rotating magnetic field and the spiral separation channel cooperate with each other, and the magnetic particles in the channel are subjected to periodic magnetic field forces and magnetophoretically move along the direction of rotation of the rotating magnetic field. The rotating magnetic field generated by the three-phase winding with an oblique lower wire slot causes the magnetic particles with different characteristics in the separation channel to be subjected to different magnetic field force components along the flow direction of the carrier liquid, which will produce a magnetophoretic velocity difference and achieve effective separation.

[0004] The external magnetic field used in magnetophoresis separation is the rotating magnetic field inside the cylindrical hollow structure of the base, and the separation channel is a spiral microtube. A hollow cylindrical base is used, the inner wall of the base is provided with a lower wire groove, the three-phase winding is embedded in the lower wire groove, and a spiral microtube is provided in the cylindrical space as a separation channel.

[0005] Although effective magnetophoretic separation can be achieved, the above scheme has the following problems: (1) Under the action of the rotating magnetic field, the magnetic particles move along the spiral separation channel, and the motion trajectory is relatively complex. (2) This is a closed structure. The separation channel is inside the cylindrical structure. Under the transparent condition of the microtube separation channel, it is not conducive to placing test instruments to observe and record the real-time magnetophoretic movement of the magnetic particles in the separation channel. (3) For the cylindrical spiral separation channel, if the lead or diameter of the spiral needs to be changed, the spiral fixing groove of the separation channel needs to be reprocessed, which brings inconvenience to the separation work. (4) In the magnetophoretic separation method of the rotating magnetic field dynamic magnetophoretic separation device, the infusion pump is always turned on during the separation of magnetic particles, and the carrier flow rate and the magnetophoretic movement of the magnetic particles are superimposed on each other, which will have a certain impact on the particle separation results. Summary of the invention

[0006] In view of this, the present invention provides a circulating magnetic field dynamic magnetophoresis separation device and method to overcome the technical problems of existing dynamic magnetophoresis separation devices during magnetophoresis separation, such as complex motion trajectories of magnetic particles, inability to observe and record the real-time magnetophoresis motion of magnetic particles in the separation channel, inability to cope with changes in separation channel size, and inability to obtain accurate particle separation results.

[0007] To this end, the present invention provides the following technical solutions:

[0008] On the one hand, the present invention provides a circulating magnetic field dynamic magnetophoresis separation device, comprising: a base, a separation channel, a three-phase winding, a three-phase transformer, a frequency conversion circuit, a UV / Vi ultraviolet / visible light detector, a waste liquid collection bottle, a three-way valve and an infusion pump; the base is a linear base, the base has a lower wire groove, and the three-phase winding is embedded in the lower wire groove; when the three-phase winding is supplied with a three-phase alternating current, a moving magnetic field is generated on the upper part of the linear base, and the magnetic induction intensity of the magnetic field changes according to the law of a sine function curve. When the three-phase current changes periodically, a circulating magnetic field is formed on the upper part of the base;

[0009] The separation channel is a linear separation channel in a tubular shape; the separation channel is located at the upper part of the base; when the circulating magnetic pole moves past the magnetic particles in the separation channel, the particles will be affected by the magnetic field force and undergo magnetophoresis.

[0010] Furthermore, it also includes: a testing instrument arranged around the separation channel.

[0011] Furthermore, the base is a four-pole three-phase winding base structure. The four poles generated by the three-phase winding are two pairs of N-pole and S-pole magnetic poles. The magnetic field on the upper part of the base travels and circulates in a straight line direction as N-pole, S-pole, N-pole, and S-pole, and differs by 90° in space.

[0012] Furthermore, the base is a silicon steel base.

[0013] Furthermore, the device comprises: a three-phase winding base, a transparent acrylic plate is fixed on the upper part of the base, a separation channel positioning groove is provided on the acrylic plate, and the separation channel is embedded in the positioning groove.

[0014] Furthermore, the device includes: two three-phase winding bases, which are placed in mirror symmetry above and below; two transparent acrylic plates of the same specifications are fixed between the two bases to form a bonding surface, a semi-cylindrical groove is opened on the lower bonding surface, and a semi-cylindrical groove is also opened on the upper bonding surface, and the separation channel is embedded in the positioning groove formed by the two semi-cylindrical grooves.

[0015] In another aspect, the present invention further provides a method for separating magnetic particles using the above-mentioned circulating magnetic field dynamic magnetophoresis separation device, comprising:

[0016] Preparation of magnetic particles with the same magnetic susceptibility and different particle sizes;

[0017] The infusion pump is turned on, and the magnetic particles to be separated are injected into the carrier liquid through the three-way valve using an injection syringe. When the magnetic particles flow into the separation channel driven by the carrier liquid, the infusion pump is turned off to make the carrier liquid flow rate zero;

[0018] When the power supply of the linear three-phase winding base is turned on, a circulating magnetic field will be generated on the upper part of the base; the frequency, magnetic induction intensity and carrier flow rate parameters of the circulating magnetic field are adjusted to perform magnetic particle magnetic force traction magnetophoresis separation;

[0019] When the magnetic particles achieve magnetophoretic separation in the separation channel, the power supply of the linear three-phase winding base is disconnected, the circulating magnetic field disappears, the infusion pump is turned on, and the carrier liquid carries the magnetic particles out of the separation channel at a certain flow rate.

[0020] Furthermore, the method also includes: using a testing instrument to observe the separation of particles through a transparent separation channel.

[0021] Furthermore, the method also includes: testing the separation effect using an ultraviolet-visible light detector.

[0022] Advantages and positive effects of the present invention:

[0023] The circulating magnetic field dynamic magnetophoresis separation device in the present invention places a linear microtube separation channel on the upper part of a linear base to form an open structure, thereby realizing magnetophoresis separation of magnetic particles with different characteristics. The linear circulating magnetic field causes the magnetic particles to move in a straight line as a whole in the separation channel, and can complete dynamic magnetophoresis separation of magnetic particles in the linear separation channel. The external magnetic field source is optimized, and an open structure is adopted to allow dynamic magnetophoresis of magnetic particles in the linear separation channel. It is beneficial to place the test instrument around the separation channel to observe and monitor the real-time separation of magnetic particles in the separation channel. Compared with the spiral separation channel, the use of a linear separation channel is easy to process and fix, which facilitates the implementation of magnetophoresis separation.

[0024] The magnetophoretic separation method proposed on the basis of the circulating magnetic field dynamic magnetophoretic separation device is that after the magnetic particles flow into the separation channel, the infusion pump is turned off to control the carrier liquid to stop flowing. Under the traction of the magnetic field force of the circulating magnetic field, the magnetic particles with different characteristics are separated by magnetophoretic motion. Then the infusion pump is turned on again, and the separated magnetic particles are driven by the carrier liquid to flow out of the separation channel in sequence. This method reduces the influence of the carrier liquid flow rate on magnetophoretic separation. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative labor.

[0026] Figure 1 Schematic diagram of the structure of a circulating magnetic field dynamic magnetophoresis separation device in an embodiment of the present invention;

[0027] Figure 2 It is a schematic diagram of a linear base structure in an embodiment of the present invention;

[0028] Figure 3 Schematic diagram of the generation of a linear circulating magnetic field in an embodiment of the present invention;

[0029] Figure 4 This is a single-layer chain connection diagram of a three-phase winding on a linear base in an embodiment of the present invention;

[0030] Figure 5 This is a schematic diagram of the separation channel structure in an embodiment of the present invention;

[0031] Figure 6 This is a schematic diagram of a three-phase winding base and a separation channel structure in an embodiment of the present invention;

[0032] Figure 7It is a schematic diagram of another structure of a three-phase winding base and a separation channel in an embodiment of the present invention;

[0033] Figure 8 Schematic diagram of separation results of dynamic magnetophoresis separation in a circulating magnetic field in an embodiment of the present invention;

[0034] In the figure, 1-base, 2-separation channel, 3-three-phase winding, 3-1-A phase winding, 3-2-B phase winding, 3-3-C phase winding, 4-three-phase transformer, 5-frequency conversion circuit, 6-UV / Vi ultraviolet / visible light detector, 7-waste liquid collection bottle, 8-three-way valve, 9-infusion pump, 10-down line slot, 11-equivalent magnetic pole, 12-acrylic plate, 13-separation channel positioning slot, 14-liquid carrier inlet, 15-liquid carrier outlet, 16-injection syringe, 17-magnetic induction intensity. DETAILED DESCRIPTION

[0035] In order to enable those skilled in the art to better understand the scheme of the present invention, the technical scheme in the embodiments of the present invention will be clearly and completely described below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work should fall within the scope of protection of the present invention.

[0036] It should be noted that the terms "first", "second", etc. in the specification and claims of the present invention and the above-mentioned drawings are used to distinguish similar objects, and are not necessarily used to describe a specific order or sequence. It should be understood that the data used in this way can be interchanged where appropriate, so that the embodiments of the present invention described herein can be implemented in an order other than those illustrated or described herein. In addition, the terms "including" and "having" and any variations thereof are intended to cover non-exclusive inclusions, for example, a process, method, system, product or device that includes a series of steps or units is not necessarily limited to those steps or units that are clearly listed, but may include other steps or units that are not clearly listed or inherent to these processes, methods, products or devices.

[0037] like Figure 1 As shown, the circulating magnetic field dynamic magnetophoresis separation device in the embodiment of the present invention includes: a base 1, a separation channel 2, a three-phase winding 3, a three-phase transformer 4, a frequency conversion circuit 5, a UV / Vi ultraviolet / visible light detector 6, a waste liquid collection bottle 7, a three-way valve 8 and an infusion pump 9.

[0038] After the three-phase winding 3 is connected to the three-phase sinusoidal alternating current, a circulating magnetic field will be generated on the upper part of the base 1, and the linear microtube separation channel 2 is placed on the upper part of the base 1. Under the joint action of the injection syringe 16, the three-way valve 8 and the infusion pump 9, the sample is input into the separation channel 2, and the magnetic particles in the injection move in a straight line in the separation channel 2 driven by the carrier liquid. The three-phase winding base that generates the circulating magnetic field and the linear separation channel support each other. When the circulating magnetic field passes near the magnetic particles in the separation channel, the particles will be affected by the magnetic field force generated by the circulating magnetic field and move magnetically at a certain speed along the direction of movement of the magnetic field. The carrier liquid flowing out of the separation channel 2 is detected by a UV / Vi ultraviolet / visible light detector, and the waste liquid is recovered by a waste liquid collection bottle.

[0039] The three-phase winding base structure is externally connected to a frequency conversion circuit 5, which can adjust the frequency of the three-phase AC voltage applied to the three-phase winding 3, thereby controlling the frequency of the circulating magnetic field acting on the magnetic particles in the separation channel. The three-phase winding base structure is externally connected to a three-phase transformer 4, which can change the magnitude of the three-phase AC voltage applied to the three-phase winding 3, affect the magnitude of the magnetic induction intensity in the separation channel 2, and control the magnetic field force exerted on the magnetic particles in the separation channel 2. The circulating magnetic field generated by this three-phase winding base causes magnetic particles with different characteristics to be subjected to different magnetic field force components along the flow direction of the carrier liquid, resulting in a magnetophoresis velocity difference, thereby achieving effective separation of magnetic particles.

[0040] like Figure 2 As shown, the base 1 in the embodiment of the present invention is a silicon steel linear base, and there is a lower wire groove 10 on the base 1, and the three-phase winding 3 is embedded in the lower wire groove 10. When the three-phase alternating current is passed through the three-phase winding 3, a moving magnetic field is generated on the upper part of the linear silicon steel base 1, and the magnetic induction intensity of the magnetic field changes according to the law of the sine function curve. The three-phase currents are respectively U phase, V phase and W phase. Assuming that the number of magnetic poles p = 1, the phase of the U phase current at ωt = 0° Then the magnetic field generated on the upper part of the linear base when ωt = 150° is as follows Figure 3 As shown. At this moment, the U-phase current i1>0, the V-phase current i2>0, and the W-phase current i3<0, which is equivalent to an equivalent magnetic pole S pole 11 on the upper part of the base 1. The three-phase AC current continues to change, and the S pole moves to the left. The dotted line shows the magnetic induction intensity 17. When the three-phase current changes in one cycle, the magnetic pole S pole passes through the upper part of the base once from right to left along a straight line. In this way, when the three-phase current changes periodically, a circulating magnetic field will be formed on the upper part of the base 1.

[0041] For the 4-pole three-phase winding base structure, it can be equivalent to 4 magnetic poles running along a straight line on the upper part of the base 1. The three-phase winding 3 includes the A-phase winding 3-1, the B-phase winding 3-2, and the C-phase winding 3-3. The 4 poles generated are two pairs of N-pole and S-pole magnetic poles. The magnetic field on the upper part of the base runs and circulates along a straight line in the distribution of N-pole-S-pole-N-pole-S-pole, and the phases differ by 90° in space. The single-layer chain connection method of the three-phase winding with 4 poles and 24 slots to generate a circulating magnetic field is as follows: Figure 4 shown.

[0042] The material of the separation channel is PEEK plastic. The linear separation channel is Figure 5 As shown, a transparent acrylic plate 12 is fixed on the upper part of the silicon steel base, and a separation channel positioning groove 13 is provided on the acrylic plate 12. The separation channel 2 is tubular, with an outer diameter of 1.59 mm and an inner diameter of 0.75 mm. The separation channel is directly embedded in the positioning groove 13, with a carrier liquid inlet 14 on the left and a carrier liquid outlet 15 on the right. When the circulating magnetic pole moves near the magnetic particles in the separation channel, the particles will be affected by the magnetic field force and undergo magnetophoresis.

[0043] Three-phase winding base and separation channel structure embodiment 1:

[0044] The three-phase winding base and separation channel structure of Example 1 are as follows: Figure 6 As shown in the figure, a three-phase winding silicon steel base 1 is provided, a transparent acrylic plate 12 is fixed on the upper part of the base, a separation channel positioning groove 13 is provided on the acrylic plate 12, and the separation channel 2 is embedded in the positioning groove 13. With this unilateral structure, the magnetic particles in the separation channel will be subjected to a large normal magnetic pull generated by the circulating magnetic field.

[0045] Three-phase winding base and separation channel structure embodiment 2:

[0046] The three-phase winding base and separation channel structure of Example 2 are as follows: Figure 7 As shown. Two three-phase winding silicon steel bases 1 are placed symmetrically in upper and lower mirror images. Two transparent acrylic plates 12 of the same specifications are fixed between the bases. A linear separation channel 2 is set on the joint surface of the acrylic plate 12. Specifically, a semi-cylindrical groove is opened on the lower joint surface, and a semi-cylindrical groove is also opened on the upper joint surface, and the tubular separation channel is embedded in the positioning groove formed by the two semi-cylindrical grooves. With this bilateral structure, the normal magnetic pull of the magnetic particles in the separation channel under the circulating magnetic field can offset each other.

[0047] The linear three-phase winding base dynamic magnetophoresis device can be used to perform dynamic magnetophoresis separation of magnetic particles, and the specific method includes:

[0048] S1. Prepare magnetic particles with the same magnetic susceptibility but different particle sizes.

[0049] S2. Turn on the infusion pump and inject the magnetic particles to be separated into the carrier liquid through the three-way valve using an injection syringe. When the magnetic particles flow into the separation channel driven by the carrier liquid, turn off the infusion pump to make the carrier liquid flow rate zero.

[0050] S3, when the power supply of the linear three-phase winding base is turned on, a circulating magnetic field will be generated on the upper part of the base; the frequency, magnetic induction intensity, carrier flow rate and other parameters of the circulating magnetic field are adjusted to perform magnetic particle magnetic field force traction magnetophoresis separation;

[0051] Under the action of the linear motion circulating magnetic field, when the equivalent magnetic pole passes near the magnetic particles in the separation channel, the particles are pulled by the magnetic field force of the circulating magnetic field and undergo magnetophoretic motion along the flow direction of the carrier liquid, resulting in magnetophoretic displacement.

[0052] S4. Use a microscope or other testing instruments to observe the separation of particles through a transparent separation channel.

[0053] Magnetic particles with different characteristics will be subjected to different components of magnetic field force along the flow direction of the carrier liquid, thus resulting in differences in magnetophoretic velocity and displacement, and different positions in the separation channel.

[0054] S5. After the magnetic particles have achieved magnetophoretic separation in the separation channel, the power supply of the linear three-phase winding base is disconnected, the circulating magnetic field disappears, the infusion pump is turned on, and the carrier liquid carries the magnetic particles out of the separation channel at a certain flow rate.

[0055] S6. Use an ultraviolet-visible light detector (UV / Vis) to test the separation effect.

[0056] Magnetic particles with different characteristics have different retention times in the separation channel, and the time they flow out of the separation channel will be different, thus achieving magnetophoretic separation.

[0057] For ease of understanding, the above-mentioned magnetophoresis separation method is described below with a specific example.

[0058] In the circulating magnetic field dynamic magnetophoresis separation device, specifically according to Example 2, the magnetic field force of the magnetic particles in the separation channel is pulled magnetophoresis motion along a linear motion by the circulating magnetic field to separate magnetic particles of different particle sizes. Core-shell polystyrene magnetic particles of different particle sizes are prepared to obtain mixed samples with magnetic particle diameters of 6.3μm and 3.5μm. Magnetic particles of different particle sizes have the same magnetic susceptibility and show superparamagnetic response characteristics. The working state and related parameters of the three-phase winding circulating magnetic field magnetophoresis separation device are set. The direction of travel of the circulating magnetic field is the same as the flow direction of the carrier liquid, and the injection volume of the magnetic particle mixed sample is 20μl. The frequency of the rotating magnetic field is adjusted to 6Hz, the three-phase voltage added to the three-phase winding is 18V, deionized water is used as the carrier liquid, and the carrier liquid flow rate is 0.1ml / min. Turn on the infusion pump, inject the magnetic particle mixed sample into the carrier liquid through the three-way valve, and after the mixed sample flows into the separation channel driven by the carrier liquid, turn off the infusion pump. When the power of the linear three-phase winding base is turned on, the circulating magnetic field moves linearly near the magnetic particles in the separation channel. The particles are pulled by the magnetic field force of the circulating magnetic field and undergo magnetophoretic displacement along the flow direction of the carrier liquid. Magnetic particles with different characteristics have different magnetophoretic displacements, and their positions in the separation channel are front and back. After 12 minutes, the magnetic particles achieve magnetophoretic separation in the separation channel. Disconnect the power of the linear three-phase winding base, the circulating magnetic field disappears, turn on the infusion pump, and the separated magnetic particles are driven by the carrier liquid to flow out of the separation channel and pass through the UV-visible light detector. The separation is tested with a UV-visible light detector, and the separation results of the magnetic particles are shown as follows. Figure 8 As shown. Magnetic particles with diameters of 6.3μm (peak a) and 3.5μm (peak b) were separated. These two magnetic particles with the same magnetic susceptibility and different diameters can be completely separated in the circulating magnetic field dynamic magnetophoresis separation device to meet the separation and analysis requirements. The circulating magnetic field dynamic magnetophoresis separation method achieves the separation of magnetic particles with good resolution.

[0059] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit it. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the aforementioned embodiments, or replace some or all of the technical features therein with equivalents. However, these modifications or replacements do not cause the essence of the corresponding technical solutions to deviate from the scope of the technical solutions of the embodiments of the present invention.

Claims

1. A circulating magnetic field dynamic magnetophoresis separation device, comprising: A base, a separation channel, a three-phase winding, a three-phase transformer, a frequency conversion circuit, a UV / Vi ultraviolet / visible light detector, a waste liquid collection bottle, a three-way valve and an infusion pump; characterized in that the base is a linear base, the base has a lower wire groove, the three-phase winding is embedded in the lower wire groove, and a linear arrangement of the three-phase winding is formed; when the three-phase winding is passed through a three-phase sinusoidal alternating current, a moving magnetic field is generated on the upper part of the linear base, and the magnetic induction intensity of the magnetic field changes according to the law of the sinusoidal function curve. When the three-phase current changes periodically, a circulating magnetic field will be formed on the upper part of the base; The separation channel is a linear separation channel in a tubular shape; the separation channel is located at the upper part of the base; when the circulating magnetic pole moves near the magnetic particles in the separation channel, the particles will be affected by the magnetic field force, pulling the magnetic particles to undergo magnetophoresis; When performing dynamic magnetophoretic separation of magnetic particles, the circulating magnetic field dynamic magnetophoretic separation device prepares magnetic particles with the same magnetic susceptibility and different particle sizes; turns on the infusion pump, and injects the magnetic particles to be separated into the carrier liquid through the three-way valve using an injection syringe. When the magnetic particles flow into the separation channel driven by the carrier liquid, the infusion pump is turned off to make the carrier liquid flow rate zero; the power supply of the linear three-phase winding base is turned on, and a circulating magnetic field is generated on the upper part of the base; the frequency, magnetic induction intensity and carrier liquid flow rate parameters of the circulating magnetic field are adjusted to perform magnetic particle magnetic field force traction magnetophoretic separation; when magnetic particles with different characteristics magnetophoretically move at different speeds in the separation channel, magnetophoretic separation is achieved through the speed difference, the power supply of the linear three-phase winding base is disconnected, the circulating magnetic field disappears, the infusion pump is turned on, and the carrier liquid carries the magnetic particles with different characteristics at a certain flow rate and flows out of the separation channel at different times.

2. A circulating magnetic field dynamic magnetophoresis separation device according to claim 1, characterized in that: Also includes: A testing instrument is disposed around the separation channel.

3. The circulating magnetic field dynamic magnetophoresis separation device according to claim 1, characterized in that: The base is a four-pole three-phase winding base structure. The four poles generated by the three-phase winding are two pairs of N-pole and S-pole magnetic poles. The magnetic field on the upper part of the base travels and circulates in a straight line direction as N-pole, S-pole, N-pole, S-pole, and is spaced 90° apart.

4. The circulating magnetic field dynamic magnetophoresis separation device according to claim 1, characterized in that: The base is a silicon steel base.

5. The circulating magnetic field dynamic magnetophoresis separation device according to claim 4, characterized in that: The device comprises: a three-phase winding base, a transparent acrylic plate is fixed on the upper part of the base, a separation channel positioning groove is provided on the acrylic plate, and the separation channel is arranged in the positioning groove in an embedded manner.

6. The circulating magnetic field dynamic magnetophoresis separation device according to claim 4, characterized in that: The device comprises: two three-phase winding bases, which are placed in mirror symmetry up and down; two transparent acrylic plates of the same specification are fixed between the two bases to form a bonding surface, a semi-cylindrical groove is opened on the lower bonding surface, and a semi-cylindrical groove is also opened on the upper bonding surface, and a separation channel is set in a positioning groove formed by the two semi-cylindrical grooves in an embedded manner.

7. The circulating magnetic field dynamic magnetophoresis separation device according to claim 1, characterized in that: Also includes: Use the testing instrument to observe the separation of particles through the transparent separation channel.

8. The circulating magnetic field dynamic magnetophoresis separation device according to claim 1, characterized in that: Also includes: The separation was tested using a UV-Vis detector.

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