Microorganism filtration capture device

Abstract

The application discloses a microbial filtering and capturing device, which comprises a shell, an upper shell and a lower shell, a cavity is formed by the upper shell and the lower shell, the upper shell is provided with a liquid outlet, the bottom of the lower shell is provided with a columnar part, the bottom of the columnar part is provided with an attachment part, a container is connected to the attachment part, the side of the columnar part is provided with a liquid inlet, a filter membrane is arranged in the cavity and divides the cavity into an upper cavity and a lower cavity, a liquid inlet flow channel extends from the liquid inlet and penetrates into the lower cavity, a guide flow channel extends from the attachment part and penetrates into the lower cavity, a valve part is used for controlling the opening and closing of the liquid inlet flow channel and the guide flow channel, a fluid promoting part is arranged on the upper shell and is used for applying pulse pressure to fluid in the cavity, and fluid in the upper cavity can pass through the filter membrane and enter the lower cavity by providing pulse pressure to the cavity through the fluid promoting part.

Description

Technical Field

[0001] The present invention relates to a filtering device, in particular to a device for filtering and capturing microorganisms. Background Art

[0002] It is well known that the content of microorganisms such as bacteria in water used in food production needs to be controlled within a small range. Therefore, before use, water samples need to be taken to detect the type and number of microorganisms in the water. The specific detection process is: water is passed through a filter membrane, which captures (or is called) the microorganisms and only allows the aqueous solution to pass through. The captured microorganisms are then introduced into a container containing culture medium, cultured in the culture medium, and then tested through biochemical methods to determine the type and number of microorganisms.

[0003] In the prior art, the operation of filtering and capturing microorganisms is usually performed in a sterile room. However, this operation is still exposed to the air, and microorganisms that may exist in the air may contaminate the microorganisms captured by the filter membrane.

[0004] To prevent the filtering and capturing of microorganisms from being exposed to the external air, a conventional filtering and capturing device is provided. The device comprises a housing, a filter membrane disposed within the housing, an attachment portion formed below the housing, a liquid inlet formed on one side of the housing, a liquid outlet formed on the top of the housing, an inlet channel extending from the inlet to below the filter membrane, an outlet channel extending from the outlet to above the filter membrane, and a flow guide channel extending from below the filter membrane to the attachment portion. A container containing culture medium is attached to the attachment portion of the housing. During use, a water sample passes through the inlet, the inlet channel, the filter membrane, the outlet channel, and finally out of the outlet. Microorganisms in the water sample are captured by the filter membrane and adhere to the lower surface of the filter membrane. After the water sample is filtered, the device and container are inverted, and the culture medium in the container flows through the flow guide channel to the bottom of the filter membrane, thereby causing microorganisms attached to the bottom of the filter membrane to fall off and enter the culture medium. The device and container are then placed upright, so that the microorganisms are immersed in the culture medium in the container. It is easy to understand that the above device prevents the microorganisms from being exposed to the outside air during the capture process and the process of being immersed in the culture solution, thereby effectively avoiding the interference of microorganisms in the outside air.

[0005] However, the above device has the following disadvantages when used:

[0006] 1. During filtration, a certain pressure difference needs to be maintained between the upper and lower sides of the filter membrane. However, this pressure difference causes the captured microorganisms to adhere to the lower surface (bottom) of the filter membrane with a greater adhesion force. After the filtration is completed and the device is inverted, although the culture medium can infiltrate the filter membrane, due to the strong adhesion of the microorganisms, a considerable number of microorganisms will not fall off the filter membrane. Although the operator attempts to increase the microorganism removal rate by shaking, the improvement in the removal rate by shaking is still limited.

[0007] 2. After filtering the water sample, before inverting the device, close the liquid outlet and inlet channels to prevent the culture medium from flowing out of them during inversion. During inversion, the long and narrow flow channel makes it difficult for the culture medium in the container to flow through the channel to the filter membrane due to the air resistance and the channel walls. Furthermore, after the device is upright, the culture medium that has flowed to the filter membrane will also have difficulty flowing back into the container.

[0008] 3. Since each flow channel is a long and narrow flow channel, it is difficult to clean the inside of the device after completing the microbial inspection operation.

[0009] 4. Each flow channel is controlled by a linear action valve to control the on and off of each flow channel. However, the linear action valve is prone to misoperation and the structure is not compact enough. Summary of the Invention

[0010] In response to the above technical problems existing in the prior art, an embodiment of the present invention provides a microorganism filtration and capture device.

[0011] To solve the above technical problems, the technical solutions adopted in the embodiments of the present invention are:

[0012] A microorganism filtration and capture device, comprising:

[0013] The housing comprises an upper shell and a lower shell butted together at an edge, the upper shell and the lower shell enclosing a cavity; a liquid outlet communicating with the cavity is formed at the top of the upper shell, a columnar portion is formed at the bottom of the lower shell, an attachment portion is formed at the bottom of the columnar portion, a container containing culture fluid is connected to the attachment portion, and a liquid inlet is formed on the side of the columnar portion;

[0014] a filter membrane disposed in the cavity with its edge clamped between the edges of the upper shell and the lower shell, the filter membrane dividing the cavity into an upper cavity and a lower cavity;

[0015] a liquid inlet channel extending from the liquid inlet and passing through the lower chamber;

[0016] a diversion channel extending from the attachment portion through to the lower cavity;

[0017] A valve component is used to selectively control the opening and closing of the liquid inlet flow channel and the diversion flow channel; wherein:

[0018] The upper shell is provided with a fluid actuating component for applying pulse pressure to the fluid in the cavity. The fluid actuating component provides pulse pressure to the cavity so that the fluid in the upper cavity can instantly pass through the filter membrane and enter the lower cavity.

[0019] Preferably, the fluid actuating component receives external pressure and provides pulse pressure to the fluid in the chamber by changing its volume.

[0020] Preferably, a through central groove is formed in the middle of the upper shell, and a stepped portion is formed on the groove wall of the central groove; the fluid actuating component includes:

[0021] A floating cover, the edge of which is adapted to the central groove so that the floating cover can move axially along the central groove;

[0022] a spring interposed between the floating cover and the stepped portion;

[0023] A silicone ring sheet covering the annular gap formed by the floating cover and the central groove; wherein:

[0024] The floating cover is pressed to move axially downward to reduce the volume of the upper chamber and provide pulse pressure to the fluid in the chamber. After the pressure is released, the spring moves the floating cover upward to increase the volume of the upper chamber.

[0025] Preferably, a hollow portion is formed in the eccentric area of ​​the upper shell, and the fluid actuating component includes a sac covering the hollow portion and protruding outward, and the sac is made of an elastic material; wherein:

[0026] The volume of the bladder is reduced by pressing the bladder so as to provide a pulse pressure to the fluid in the cavity.

[0027] Preferably, the liquid inlet is formed in the middle of the floating cover.

[0028] Preferably, the valve component comprises:

[0029] A valve cavity radially penetrates the columnar portion and is coaxial with and interpenetrates the liquid inlet. The lower end of the liquid inlet channel penetrates the cavity wall at the front of the valve cavity to form a port. The diversion channel penetrates the cavity wall corresponding to the middle portion of the valve cavity to form upper and lower ports.

[0030] A valve core is extended into the valve cavity, and a head of the valve core extends toward the liquid inlet. A blind hole is formed on the end surface of the head of the valve core. A first front valve hole and a second front valve hole are formed on the outer periphery of the valve core at a position corresponding to the port of the liquid inlet channel, and radially penetrate to the blind hole, and the first front valve hole and the second front valve hole are arranged at a first angle thereto; a first rear valve hole and a second rear valve hole are formed on the outer periphery of the valve core at a position corresponding to the port of the diversion channel, and the first rear valve hole and the second rear valve hole are arranged at a second angle thereto;

[0031] A knob is mounted on the tail of the valve core to receive torque and drive the valve core to rotate; wherein:

[0032] The first angle and the second angle are arranged according to the following rules:

[0033] When the valve core rotates to the point where the first front valve hole is opposite to the port of the liquid inlet channel, the first rear valve hole and the second rear valve hole are both misaligned with the two ports of the diversion channel;

[0034] When the valve core rotates to the point where the first rear valve hole is opposite to the two ports of the diversion channel, the ports of the liquid inlet channel are all misaligned with the first front valve hole and the second front valve hole and are circumferentially located between them;

[0035] When the valve core rotates to the point where the second rear valve hole is opposite to the two ports of the diversion flow channel, the port of the liquid inlet flow channel is opposite to the second front valve hole.

[0036] Preferably, the filter membrane is covered with mesh both above and below.

[0037] Preferably, a silicone sealing gasket is provided between the upper shell and the lower shell at the periphery of the edge of the filter membrane.

[0038] Preferably, the upper end of the diversion channel passes through to the center of the lower cavity, the bottom of the lower cavity is constructed into a structure with a low middle and high edges, and the liquid inlet channel is located on one side of the diversion channel.

[0039] Preferably, the attachment portion is formed with a plurality of circumferentially arranged elastic catches for engaging with the upper end of the container.

[0040] Compared with the prior art, the beneficial effects of the microorganism filtration and capture device provided by the embodiments of the present invention are:

[0041] 1. The device can improve the membrane filtration of microorganisms.

[0042] 2. The device can improve the fluidity of the culture solution, thereby facilitating the culture solution to infiltrate the microorganisms and return to the container.

[0043] 3. The internal flow channel and cavity of the device are easy to clean.

[0044] 4. The valve components of the device are compact in structure and easy to operate. BRIEF DESCRIPTION OF THE DRAWINGS

[0045] Figure 1 A main cross-sectional view of a microorganism filtration and capture device provided in an embodiment of the present invention (the fluid actuating component is of the first structure).

[0046] Figure 2 for Figure 1 A-direction view.

[0047] Figure 3 A main cross-sectional view of a microorganism filtration and capture device provided in an embodiment of the present invention (the fluid actuating component is the second structure).

[0048] Figure 4 for Figure 3 B-direction view.

[0049] Figure 5 This is a view of the usage status of the microorganism filtration and capture device provided by an embodiment of the present invention (the liquid inlet channel is connected and the diversion channel is cut off).

[0050] Figure 6 for Figure 5 CC cross-sectional view.

[0051] Figure 7 A diagram of the microorganism filtration and capture device provided by an embodiment of the present invention in use (the liquid inlet channel is cut off and the diversion channel is connected).

[0052] Figure 8 for Figure 7 DD cross-sectional view

[0053] Figure 9 An embodiment of the present invention provides a view of a microorganism filtration and capture device in a tilted and inverted state.

[0054] Figure 10 An embodiment of the present invention provides a microbial filtration and capture device in use (liquid inlet channel open, diversion channel open).

[0055] Figure 11 for Figure 10 EE cross-sectional view.

[0056] In the picture:

[0057] 10-housing; 11-upper housing; 12-lower housing; 13-upper chamber; 14-lower chamber; 15-columnar portion; 16-liquid inlet; 17-liquid outlet; 171-cover; 18-attachment portion; 181-elastic claw; 19-tube; 21-liquid inlet channel; 22-diversion channel; 30-filter membrane; 31-mesh; 40-valve component; 41-valve core; 421-first front valve hole; 422-second front valve hole Valve hole; 431-first rear valve hole; 432-second rear valve hole; 44-knob; 50-fluid actuating component; 51-center groove; 52-floating cover; 53-spring; 54-silicone ring; 50'-fluid actuating component; 51'-hollow part; 52'-bag; 60-silicone sealing gasket; 70-fastener; 100-device; 200-container; 201-sealing plug; 202-culture medium. DETAILED DESCRIPTION

[0058] In order to enable those skilled in the art to better understand the technical solution of the present invention, the present invention is described in detail below with reference to the accompanying drawings and specific embodiments.

[0059] An embodiment of the present invention discloses a microorganism filtration and capture device 100 , which is used to filter water samples used for food in a non-exposure manner, thereby capturing microorganisms in the water sample and immersing the water sample in a culture solution 202 in a container 200 .

[0060] like Figure 1 As shown, the filtering and capturing device 100 includes: a housing 10, a filter membrane 30, a liquid inlet channel 21, a diversion channel 22, a valve component 40, and fluid actuating components 50, 50'.

[0061] The housing 10 is a split structure, comprising an upper shell 11 and a lower shell 12. Both upper shell 11 and lower shell 12 are configured as disc-shaped structures with a central protrusion. The upper shell 11 and lower shell 12 are butted together at their edges and secured by fasteners 70. The butted upper shell 11 and lower shell 12 form a cavity within the housing 10. The upper shell 11 and lower shell 12 can be injection molded from a food-grade plastic material, with the cavity walls being designed to be as smooth as possible to minimize the adhesion of microorganisms and particulate matter.

[0062] A semipermeable membrane or filter cloth can be selected as the filter membrane 30, so that the pore size of the micropores of the filter membrane 30 is as large as possible while meeting the requirements of intercepting and capturing microorganisms, so as to increase the smoothness of the fluid passing through and thus increase the filtration efficiency. Both surfaces of the filter membrane 30 are covered with a mesh 31, which is used to limit the filter membrane 30 from excessive deformation under the action of pressure difference during filtration, that is, the mesh 31 is used to improve the rigidity of the filter membrane 30. The edges of the filter membrane 30 and the mesh 31 are clamped between the edges of the upper shell 11 and the lower shell 12, so that the filter membrane 30 divides the cavity into an upper cavity 13 and a lower cavity 14. A silicone sealing gasket 60 is provided between the edges of the upper shell 11 and the lower shell 12 on the periphery of the filter membrane 30 to prevent the fluid from leaking through the joint between the upper shell 11 and the lower shell 12.

[0063] A liquid outlet 17 is formed at the upper shell 11, and the liquid outlet 17 passes through the upper cavity 13. When filtering the water sample, the liquid outlet 17 is connected to the wastewater collector (for collecting the filtered water sample). A columnar portion 15 is formed at the bottom of the lower shell 12, and an attachment portion 18 is formed at the bottom of the columnar portion 15. The attachment portion 18 has a structure adapted to fit the container 200 mouth of the container 200 for holding the culture solution 202, so that the container 200 mouth of the container 200 is attached to the attachment portion 18. Specifically, the attachment portion has circumferentially arranged elastic claws 181. The container 200 mouth of the container 200 is inserted into the attachment portion 18, and the elastic claws 181 are elastically deformed and reset to be clamped to the outer edge of the container 200 mouth. Preferably, a tube body 19 extends downward from the bottom of the slot of the attachment portion 18. During the process of attaching the container 200 to the attachment portion 18, as shown Figure 5 As shown, the tube 19 passes through the sealing plug 201 at the mouth of the container 200 and extends into the container 200. In this way, during operation, there is no need to open the sealing plug 201, thereby preventing the culture solution 202 in the container 200 from being exposed to the outside air.

[0064] A liquid inlet 16 is formed on the side of the columnar portion 15, and the liquid inlet 16 is connected to a water sample supply mechanism (such as a vessel with a micro pump or a syringe). A liquid inlet channel 21 extends from the liquid inlet 16 and passes through the lower cavity 14. Preferably, the liquid inlet channel 21 is a straight channel and axially passes through the interior of the columnar portion 15. Figure 5 As shown, the water sample can flow into the lower chamber 14 through the liquid inlet 16 and the liquid inlet channel 21, flow into the upper chamber 13 through the filter membrane 30, and then flow into the waste liquid collector through the liquid outlet 17. When the water sample passes through the filter membrane 30, the microorganisms are intercepted and captured by the filter membrane 30 and at least partially adhere to the lower surface of the filter membrane 30.

[0065] It should be noted that generally, the water sample supply mechanism is used to supply water sample to the liquid inlet 16 so that the pressure in the lower chamber 14 is greater than the pressure in the upper chamber 13 , thereby maintaining the pressure difference and facilitating filtration.

[0066] The flow guide flow channel 22 axially penetrates the inside of the columnar portion 15, and preferably, the flow guide flow channel 22 is a straight flow channel, the lower end of the flow guide flow channel 22 extends to the pipe body 19, the upper end of the flow guide flow channel 22 extends and penetrates to the lower cavity 14, and the flow guide flow channel 22 is located at the axial center of the columnar portion 15, and the liquid inlet flow channel 21 is located at one side of the flow guide flow channel 22. As a preferred, the cavity bottom of the lower cavity 14 is configured as a structure with a low center and a high edge, in this way, the fluid in the lower cavity 14 can flow into the container 200 through the flow guide flow channel 22.

[0067] The valve member 40 is used to control the opening and closing of the liquid inlet flow channel 21 and the flow guide flow channel 22, and the valve member 40 is configured to control the opening and closing of the flow channel by rotating the valve core 41, and the valve member 40 includes the valve core 41 and the knob 44 for receiving the torsion to drive the valve core 41 to rotate. Specifically, a valve cavity is formed in the columnar portion 15, the valve cavity transversely penetrates the columnar portion 15, and the front end of the valve core 41 extends to and is coaxial with the liquid inlet 16, the valve core 41 extends into the valve cavity and is in close contact with the cavity wall of the valve cavity, the tail of the valve core 41 is located outside the columnar portion 15, and the knob 44 is installed on the tail of the valve core 41.

[0068] The lower end of the liquid inlet flow channel 21 penetrates the cavity wall of the front part of the valve cavity to form a port, and the flow guide flow channel 22 penetrates the middle part of the valve cavity to form two corresponding ports at the upper and lower parts. A blind hole is formed on the end face of the head of the valve core 41, the blind hole axially extends a section, and two front valve holes, i.e., the first front valve hole 421 and the second front valve hole 422, are formed on the outer periphery of the valve core 41 corresponding to the axial position of the port of the lower end of the liquid inlet flow channel 21, the first front valve hole 421 and the second valve hole are arranged at an angle, in this way, the first front valve hole 421 can be relatively penetrated with the port of the liquid inlet flow channel 21 or the second front valve hole 422 can be relatively penetrated with the port of the liquid inlet flow channel 21 by rotating the valve core 41. Two rear valve holes, i.e., the first rear valve hole 431 and the second rear valve hole 432, are formed on the valve core 41 corresponding to the axial position of the two ports of the flow guide flow channel 22, the first rear valve hole 431 and the second rear valve hole 432 are also arranged at an angle, in this way, the first rear valve hole 431 can be relatively penetrated with the two ports of the flow guide flow channel 22 or the second rear valve hole 432 can be relatively penetrated with the two ports of the flow guide flow channel 22 by rotating the valve core 41.

[0069] The above-mentioned valve member 40 controls the opening and closing of the flow channel by rotating, which makes the whole device 100 more compact in structure and more convenient to operate.

[0070] The angle relationship between the two front valve holes and the two rear valve holes in the circumferential direction is:

[0071] As Figure 5 and Figure 6As shown, the circumferential angle of the first front valve hole 421 may be set to 0°, then the angle of the second front valve hole 422 is 135°, the angle of the first rear valve hole 431 is 45°, and the angle of the second rear valve hole 432 is 135°. In other words, the valve holes are arranged in the counterclockwise order of the first front valve hole 421, the first rear valve hole 431, the second front valve hole 422 and the second rear valve hole 432 (the second front valve hole 422 and the second rear valve hole 432 are at the same angle).

[0072] Based on the above, if Figure 5 and Figure 6 As shown, the valve core 41 can be rotated to make the first front valve hole 421 opposite to the port at the lower end of the liquid inlet channel 21 so that the liquid inlet channel 21 is connected. At this time, the two rear valve holes are misaligned with the ports of the diversion channel 22, and the diversion channel 22 is cut off; Figure 7 and Figure 8 As shown, the valve core 41 can be rotated 45 degrees counterclockwise so that the first rear valve hole 431 is opposite to the end of the diversion channel 22, thereby making the diversion channel 22 conductive. At this time, the two front valve holes are misaligned with the ends of the liquid inlet channel 21, and the liquid inlet channel 21 is cut off. Figure 10 and Figure 11 As shown, the valve core 41 can be rotated 90° counterclockwise to make the second front valve hole 422 opposite to the port of the liquid inlet channel 21, and the second rear valve hole 432 opposite to the port of the diversion channel 22, so that both the liquid inlet channel 21 and the diversion channel 22 are connected.

[0073] When filtering water samples, Figure 5 As shown, by rotating the valve core 41, the liquid inlet channel 21 is opened and the diversion channel 22 is cut off. The water sample enters the lower chamber 14 through the liquid inlet 16 and the liquid inlet channel 21, flows into the upper chamber 13 through the filter membrane 30, and then flows out from the liquid outlet 17. When the water sample passes through the filter membrane 30, the microorganisms are intercepted and captured by the filter membrane 30 and at least partially adhere to the lower surface of the filter membrane 30.

[0074] After filtering the water sample, Figure 7 As shown, by rotating the valve core 41 by 45 degrees, the liquid inlet channel 21 is cut off, the diversion channel 22 is connected, and the liquid outlet 17 is blocked by the cover 171, as shown in FIG. Figure 9 As shown, the device 100 and container 200 are tilted and inverted, and the culture medium 202 in the container 200 enters the lower chamber 14 through the tube 19 and the flow channel 22 and submerges the filter membrane 30. The purpose is to immerse the microorganisms on the lower surface of the filter membrane 30 in the culture medium 202. The device 100 and container 200 can also be shaken to accelerate the immersion of the microorganisms in the culture medium 202.

[0075] After being inverted for a period of time, the device 100 and the container 200 are upright, and the fluid in the lower chamber 14 and the upper chamber 13 enters the container 200 through the diversion channel 22, thereby completing the capture and infiltration of the microorganisms, and then culturing the microorganisms for subsequent detection.

[0076] like Figure 10 and Figure 11 As shown, by continuing to rotate the valve core 4190°, the liquid inlet channel 21 and the diversion channel 22 are both opened, which is beneficial for cleaning the interior of the device 100 (described in detail later).

[0077] Fluid-actuating components 50, 50' are disposed on the upper shell 11. These components 50, 50', by receiving pressure and elastically returning to their original position, change the volume of the cavity enclosed by the upper and lower shells 11, 12. Specifically, they change the volume of the upper chamber 13, thereby applying a pulsed pressure to the fluid in the cavity. After the water sample is filtered and the device 100 is inverted, this pulsed pressure causes the pressure in the upper chamber 13 to momentarily exceed the pressure in the lower chamber 14, thereby allowing the fluid (culture fluid 202) in the upper chamber 13 to pass through the filter membrane 30 and enter the lower chamber 14. As the fluid passes through the filter membrane 30, it exerts an impact force on microorganisms attached to the lower surface of the filter membrane 30, forcing the attached microorganisms to fall off the filter membrane 30. This effectively improves the removal of microorganisms from the membrane.

[0078] Two structural types of fluid actuating components 50, 50' are described below:

[0079] The fluid actuating component 50 of the first structure

[0080] like Figure 1 and Figure 2 As shown, a central groove 51 is formed in the middle of the upper shell 11, and the central groove 51 passes through the upper cavity 13, and the groove wall of the central groove 51 has a stepped portion. The flow channel actuating component includes a floating cover 52, a spring 53 and a silicone ring 54. The floating cover 52 is placed in the central groove 51, and the edge of the floating cover 52 matches the central groove 51 so that the floating cover 52 can move up and down within the limitation of the central groove 51. The spring 53 is arranged between the floating cover 52 and the stepped portion of the central groove 51, and the silicone ring 54 covers the annular gap between the floating cover 52 and the central groove 51 to prevent fluid from leaking from the annular gap.

[0081] Based on the above, if Figure 9As shown, the upper shell 11, the floating cover 52 and the filter membrane 30 surround the upper chamber 13. When the floating cover 52 is pressed downward, the volume of the upper chamber 13 becomes smaller, thereby increasing the pressure in the upper chamber 13, and forcing the fluid in the upper chamber 13 (the culture solution 202 that enters the upper chamber 13 through the filter membrane 30) to pass through the filter membrane 30 in the opposite direction and enter the lower chamber 14. After the pressure on the floating cover 52 is released, the spring 53 resets the floating cover 52 to move upward, thereby increasing the volume of the upper chamber 13 and reducing the pressure. The fluid in the lower chamber 14 flows into the upper chamber 13 through the filter membrane 30. Repeated pressing causes the fluid to flow between the upper chamber 13 and the lower chamber 14.

[0082] By quickly pressing the floating cover 52 with a finger and slowly releasing the floating cover 52 (slowly removing the pressure), the fluid in the upper chamber 13 quickly passes through the filter membrane 30 and flows into the lower chamber 14, and then the fluid in the lower chamber 14 slowly passes through the filter membrane 30 and enters the upper chamber 13, thereby generating an impact force on the microorganisms in the direction away from the filter membrane 30, which is conducive to the microorganisms leaving the filter membrane 30.

[0083] The fluid actuating component 50 of this structure is suitable for a device 100 with a relatively large cavity volume.

[0084] The fluid actuating component 50' of the second structure

[0085] like Figure 3 and Figure 4 As shown, a hollow portion 51' is formed in an eccentric area of ​​the upper housing 11. The fluid-actuating component 50' includes a pouch 52' that covers the hollow portion 51' and protrudes outward. The pouch 52' is made of an elastic material. The upper chamber 13 is enclosed by the upper housing 11, the pouch 52', and the filter membrane 30. The cavity within the pouch 52' serves as part of the upper chamber 13.

[0086] By pressing the bag 52', the cavity inside the bag 52' is reduced, and the pressure in the upper cavity 13 is greater than the pressure in the lower cavity 14, so that the fluid in the upper cavity 13 enters the lower cavity 14 through the filter membrane 30. After the pressure on the bag 52' is released, the bag 52' is reset, and the cavity is enlarged. The pressure in the upper cavity 13 is less than the pressure in the lower cavity 14, and the fluid in the lower cavity 14 enters the upper cavity 13 through the filter membrane 30. Repeated pressing allows the fluid to flow between the upper cavity 13 and the lower cavity 14.

[0087] By quickly pressing the pouch 52' with a finger and slowly releasing the pouch 52' (slowly removing the pressure), the fluid in the upper chamber 13 quickly passes through the filter membrane 30 and flows into the lower chamber 14, and then the fluid in the lower chamber 14 slowly passes through the filter membrane 30 and enters the upper chamber 13, thereby generating an impact force on the microorganisms in a direction away from the filter membrane 30, which is conducive to the microorganisms leaving the filter membrane 30.

[0088] The fluid actuating component 50 ′ of this structure makes the volume change small, and is therefore suitable for the device 100 with a smaller cavity volume.

[0089] The above-mentioned fluid actuating components 50 , 50 ′ further have advantages in the following aspects.

[0090] 1. The culture medium 202 enters the cavity more smoothly. This is because when the container is inverted, the fluid actuating components 50, 50' are pressed to squeeze the gas in the cavity and the flow channel 22 into the container 200. When the container is reset, negative pressure is formed in the container 200, forcing the culture medium 202 into the cavity.

[0091] 2. After the device 100 and the container 200 are placed upright, the fluid actuating components 50 and 50 ′ are pressed to allow the fluid (culture solution 202 ) in the cavity to flow smoothly downward and into the container 200 .

[0092] 3. It is convenient for cleaning the interior of the device 100. The reason is that after filtration and culture, the interior of the device 100 needs to be cleaned to facilitate reuse. At this time, the container 200 is disassembled, the liquid outlet 17 is blocked, and the valve core 41 is rotated to connect the liquid inlet channel 21 and the diversion channel 22. The device 100 is immersed in the cleaning liquid. By repeatedly pressing the fluid actuating components 50, 50', the cleaning liquid is repeatedly introduced into the liquid inlet channel 21 and the diversion channel 22 through the liquid inlet 16 and the tube body 19 and repeatedly enters the cavity, thereby cleaning the channel and the cavity.

[0093] 4. The valve component 40 allows the two flow channels to have three on-off states, and cooperates with the fluid actuating components 50 and 50 ′ to facilitate demoulding of microorganisms and cleaning of the interior of the device 100 .

[0094] The above embodiments are merely exemplary embodiments of the present invention and are not intended to limit the scope of the present invention. The scope of protection of the present invention is defined by the claims. Those skilled in the art may make various modifications or equivalent substitutions to the present invention within the spirit and scope of protection of the present invention, and such modifications or equivalent substitutions shall also be deemed to fall within the scope of protection of the present invention.

Claims

1. A microorganism filtration and capture device, characterized in that: include: A housing, comprising an upper shell and a lower shell butted at an edge, wherein the upper shell and the lower shell form a cavity; The top of the upper shell is formed with a liquid outlet communicating with the cavity, the bottom of the lower shell is formed with a columnar portion, the bottom of the columnar portion is formed with an attachment portion, a container containing culture fluid is connected to the attachment portion, and a side of the columnar portion is formed with a liquid inlet; a filter membrane disposed in the cavity with its edge clamped between the edges of the upper shell and the lower shell, the filter membrane dividing the cavity into an upper cavity and a lower cavity; a liquid inlet channel extending from the liquid inlet and passing through the lower chamber; a diversion channel extending from the attachment portion through to the lower cavity; A valve component is used to selectively control the opening and closing of the liquid inlet flow channel and the diversion flow channel; wherein: The upper shell is provided with a fluid actuating component for applying pulse pressure to the fluid in the cavity. The fluid actuating component provides pulse pressure to the cavity, so that the fluid in the upper cavity can instantly pass through the filter membrane and enter the lower cavity. The fluid actuating component receives external pressure and provides pulse pressure to the fluid in the cavity by changing its volume; A through central groove is formed in the middle of the upper shell, and a stepped portion is formed on the groove wall of the central groove; the fluid actuating component includes: A floating cover, the edge of which is adapted to the central groove so that the floating cover can move axially along the central groove; a spring interposed between the floating cover and the stepped portion; A silicone ring sheet covering the annular gap formed by the floating cover and the central groove; wherein: The floating cover is pressed to move axially downward to reduce the volume of the upper chamber and provide pulse pressure to the fluid in the chamber. After the pressure is released, the spring moves the floating cover upward to increase the volume of the upper chamber.

2. The microorganism filtration and capture device according to claim 1, characterized in that: The liquid inlet is formed in the middle of the floating cover.

3. The microorganism filtration and capture device according to claim 1, characterized in that: The valve component comprises: A valve cavity radially penetrates the columnar portion and is coaxial with and interpenetrates the liquid inlet. The lower end of the liquid inlet channel penetrates the cavity wall at the front of the valve cavity to form a port. The diversion channel penetrates the cavity wall corresponding to the middle portion of the valve cavity to form upper and lower ports. A valve core is extended into the valve cavity, and a head of the valve core extends toward the liquid inlet. A blind hole is formed on the end surface of the head of the valve core. A first front valve hole and a second front valve hole are formed on the outer periphery of the valve core at a position corresponding to the port of the liquid inlet channel, and radially penetrate to the blind hole, and the first front valve hole and the second front valve hole are arranged at a first angle thereto; a first rear valve hole and a second rear valve hole are formed on the outer periphery of the valve core at a position corresponding to the port of the diversion channel, and the first rear valve hole and the second rear valve hole are arranged at a second angle thereto; A knob is mounted on the tail of the valve core to receive torque and drive the valve core to rotate; wherein: The first angle and the second angle are arranged according to the following rules: When the valve core rotates to the point where the first front valve hole is opposite to the port of the liquid inlet channel, the first rear valve hole and the second rear valve hole are both misaligned with the two ports of the diversion channel; When the valve core rotates to the point where the first rear valve hole is opposite to the two ports of the diversion channel, the ports of the liquid inlet channel are all misaligned with the first front valve hole and the second front valve hole and are circumferentially located between them; When the valve core rotates to the point where the second rear valve hole is opposite to the two ports of the diversion flow channel, the port of the liquid inlet flow channel is opposite to the second front valve hole.

4. The microorganism filtration and capture device according to claim 1, characterized in that: The top and bottom of the filter membrane are both covered with mesh sheets.

5. The microorganism filtration and capture device according to claim 1, characterized in that: A silicone sealing gasket is provided between the upper shell and the lower shell on the periphery of the edge of the filter membrane.

6. The microorganism filtration and capture device according to claim 1, characterized in that: The upper end of the diversion channel passes through to the center of the lower cavity, the bottom of the lower cavity is configured as a structure with a low middle portion and high edges, and the liquid inlet channel is located on one side of the diversion channel.

7. The microorganism filtration and capture device according to claim 1, characterized in that: The attachment portion is formed with a plurality of elastic catches arranged circumferentially for snapping with the upper end of the container.

Images