A method for enriching ammonia oxidizing bacteria

By designing an SBR reactor and gradually increasing the ammonia nitrogen concentration, AOB was successfully enriched, solving the problems of high oxygen consumption and poor enrichment effect, and achieving the effect of efficiently treating high-concentration ammonia nitrogen wastewater.

CN115849574BActive Publication Date: 2025-09-30JIANGNAN UNIV +1
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Patent Information

Application Number
CN202211465979.0
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Filing Date
2022-11-22
Publication Date
2025-09-30
Estimated Expiration
2042-11-22

AI Technical Summary

Technical Problem

The existing method for enriching ammonia-oxidizing bacteria (AOB) consumes a lot of oxygen and has poor enrichment effect. In addition, the enriched sludge cannot effectively treat high-ammonia nitrogen wastewater.

Method used

A cylindrical sequencing batch reactor (SBR) with a height of 40 cm and an inner diameter of 14 cm was used, and an insulation layer was used to maintain a temperature of 28-30°C. The dissolved oxygen was controlled at 0.5-1 mg/L. By gradually increasing the ammonia nitrogen concentration of artificial simulated wastewater to 500 mg/L, AOB was cultured in inorganic salt culture medium to inhibit the growth of other microorganisms, thereby achieving the advantageous enrichment of AOB.

Benefits of technology

It has achieved efficient enrichment of AOB, with an ammonia nitrogen removal rate of over 98% and an AOB abundance of 38.65%. It is capable of treating high-concentration ammonia nitrogen wastewater and reducing energy consumption and operating costs.

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Abstract

The present invention discloses a method for enriching ammonia-oxidizing bacteria, belonging to the field of sewage treatment technology. This method uses an SBR reactor to enrich AOB. For wastewater with high ammonia-nitrogen concentrations, the microbial ammonia-nitrogen load is gradually increased. A culture medium primarily composed of inorganic salts is used, without the addition of other carbon sources. The simple process significantly inhibits the growth of protozoa, metazoa, fungi, and heterotrophic bacteria in the sludge, favoring AOB as the dominant species in the activated sludge population and allowing them to tolerate increasingly high ammonia-nitrogen concentrations.
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