N-substituted 3,4-dihydroisoquinolin-1(2H)-one derivatives, compositions thereof and use thereof in medicine

Abstract

The present invention relates to a compound having an N-substituted 3,4-dihydroisoquinoline-1(2H)-one structure as shown in formula (I), which can be used for preventing, treating or alleviating diseases associated with soluble epoxide hydrolase (sEH), especially non-alcoholic fatty liver disease, fatty liver, steatohepatitis, liver fibrosis and cirrhosis.

Description

Technical Field

[0001] The present invention relates to the field of medicinal chemistry, in particular to N-substituted 3,4-dihydroisoquinoline-1(2H)-one derivative compounds and compositions thereof, and also to the use of the compounds and compositions thereof in the preparation of drugs for preventing, treating or alleviating non-alcoholic fatty liver disease. Background Art

[0002] Nonalcoholic fatty liver disease (NAFLD) is a common liver disease, especially with a high prevalence in developed countries. NAFLD is defined as a clinical pathological syndrome characterized by diffuse macrovesicular fatty degeneration of hepatocytes and lipid accumulation in patients without a history of excessive drinking (or alcohol intake <20g / d). The entire pathological process of the disease includes four stages: simple fatty liver, steatohepatitis, liver fibrosis, and cirrhosis. About 20%-30% of patients with nonalcoholic fatty liver may develop nonalcoholic steatohepatitis (NASH), which differs from simple fatty degeneration mainly in the presence of ballooning degeneration of hepatocytes and inflammatory damage, and may eventually progress to cirrhosis and hepatocellular carcinoma.

[0003] Soluble epoxide hydrolase (sEH) is a bifunctional homodimeric enzyme that is mainly involved in the metabolism of cholesterol and anti-inflammatory active substances epoxy fatty acid compounds. Studies have shown that the expression or activity level of sEH in NAFLD animal models is increased; long-term high-fat diet can lead to a significant increase in the protein level of sEH in the liver of mice and is associated with lipid accumulation; sEH inhibitors (TPPU and t-AUCB) or knockout of the sEH gene can significantly improve carbon tetrachloride-induced liver fibrosis and high-fat diet-induced liver lipid accumulation. These research results suggest that sEH can be used as a drug treatment target for NAFLD, and sEH inhibitors are expected to become candidate drugs for the treatment of NAFLD. The pharmacodynamic model of sEH inhibitors indicates that this class of compounds includes pharmacophores such as main adamantane and urea.

[0004] For example, CN101679258A discloses a 4-piperidinyl urea compound that can be used as a soluble epoxide hydrolase inhibitor, which can be used to treat sEH-mediated related diseases, such as hypertension, cardiovascular disease, inflammatory disease, lung disease, diabetes, etc. The common structural feature of this type of compound is that it contains a substituted alkyl-ureido-pyridin-4-yl structure as a parent core.

[0005] However, the inherent water solubility, melting point or pharmacokinetic properties of compounds containing urea structures are not conducive to drug development. The development of non-urea type sEH inhibitors is an important research direction for the development of drugs for NAFLD. Summary of the invention

[0006] In view of the shortcomings of the prior art, the inventors unexpectedly discovered a class of sEH inhibitors with a novel structure; in addition, since the sEH inhibitor does not contain a urea structure in its molecular structure, it can avoid the inherent disadvantages of drugs containing a urea structure. Specifically, the compound according to the present invention has an N-substituted 3,4-dihydroisoquinolin-1(2H)-one structure as a molecular skeleton.

[0007] Therefore, the first aspect of the present invention relates to a compound as shown in formula (I) and its racemate, stereoisomer, geometric isomer, tautomer, isotope-labeled substance, hydrate, solvate, polymorph, metabolite, prodrug, pharmaceutically acceptable salt or ester:

[0008]

[0009] Among them, R 1 ~R 4 R is independently selected from H, hydroxy, halogen, cyano, carboxyl, unsubstituted or substituted amino, unsubstituted or substituted alkyl, unsubstituted or substituted alkenyl, unsubstituted or substituted alkynyl, unsubstituted or substituted alkoxy, unsubstituted or substituted alkenyloxy, unsubstituted or substituted alkynyloxy, unsubstituted or substituted aminoalkyloxy, unsubstituted or substituted alkylcarbonyloxy, unsubstituted or substituted acyl or unsubstituted or substituted acylamino, R 5 represents H, alkyl, alkenyl or alkynyl.

[0010] According to one embodiment of the present invention, R 1 and R 2 Located at the 5-position and 8-position of 3,4-dihydroisoquinolin-1(2H)-one, the compound according to the present invention has a structure shown in formula (II):

[0011]

[0012] According to one embodiment of the present invention, R 1 and R 2 is selected from H, hydroxy, amino, unsubstituted or optionally substituted with one, two or more R a Substituted with the following groups: C 1-12 Alkyl, C 2-12 Alkenyl, C 2-12 Alkynyl, C 1-12 Alkoxy, C 2-12 Alkenyloxy, C 2-12 Alkynyloxy, amino-C 1-12 Alkoxy, where R a Selected from C 1-12 Alkyl, C 2-12 Alkenyl, C2-12 Alkynyl, unsubstituted or substituted C 6-14 Aryl, unsubstituted or substituted 5-14 membered heteroaryl.

[0013] Preferably, R 1 and R 2 is selected from H, hydroxy, unsubstituted or optionally substituted with one, two or more R a Substituted with the following groups: C 1-6 Alkyl, C 2-6 Alkenyl, C 1-6 Alkoxy, C 2-6 Alkenyloxy, amino-C 1-6 Alkoxy, where R a Selected from C 1-6 Alkyl, C 2-6 Alkenyl, unsubstituted or substituted C 6-10 Aryl, unsubstituted or substituted 5-14 membered heteroaryl.

[0014] R a The aryl and heteroaryl groups in the group, when substituted, are preferably substituted by a group selected from the group consisting of: C 1-6 Alkyl, C 2-6 Alkenyl, C 1-6 Alkoxy, C 2-6 The alkenyloxy group, carboxyl group, phenyl group or carboxylphenyl group is more preferably substituted by a group selected from the group consisting of methyl group, ethyl group, phenyl group, carboxyl group or carboxylphenyl group.

[0015] More preferably, R 1 and R 2 is selected from H, hydroxy, methoxy, isopropenyl, isopropenyloxy, geranyl, geranyloxy, 3,8-dimethylnon-6-enyl, 3,8-dimethylnon-6-enyloxy, 3,8-dimethylnon-2-enyl, 3,8-dimethylnon-2-enyloxy, ethylpyridylethyl, ethylpyridylethoxy, N-methyl-N-pyridylaminoethoxy, benzothiazolylethoxy, benzoxazolylethoxy, benzimidazolylethoxy, indolylethoxy, 2-phenyl-5-methyloxazolylethoxy, carboxybiphenylmethoxy, carboxyisopropoxybenzyloxy or carboxyisopropoxy.

[0016] Most preferably, R 1 and R 2 Each is independently selected from the following groups, wherein the wavy line Indicate the point of attachment of the group to the rest of the molecule:

[0017]

[0018] Preferably, R 3 and R 4are each independently selected from H, halogen, hydroxy, nitro, amino, cyano, unsubstituted or optionally substituted with one, two or more R b Substituted with the following groups: C 1-6 Alkyl, amino, C 1-6 Alkylcarbonylamino, C 1-6 Alkylcarbonyloxy, C 1-6 Alkenylcarbonylamino, where R b Selected from halogen, cyano, C 1-6 Alkyl, nitro, phenyl, C 3-6 Cycloalkyl.

[0019] More preferably, R 3 and R 4 Each is independently selected from the following groups, wherein the wavy line Indicate the point of attachment of the group to the rest of the molecule:

[0020]

[0021] Preferably, R 5 Indicates H or C 1-6 alkyl.

[0022] According to one embodiment of the present invention, the N-substituted 3,4-dihydroisoquinolin-1(2H)-one derivatives or compounds of formula (I) or (II) of the present invention are selected from the following compounds:

[0023]

[0024]

[0025] According to the second aspect of the present invention, the present invention also provides a pharmaceutical composition comprising a therapeutically effective amount of a compound represented by formula (I) or (II), or at least one of its racemates, stereoisomers, geometric isomers, tautomers, isotope-labeled substances, hydrates, solvates, polymorphs, metabolites, prodrugs, pharmaceutically acceptable salts or esters.

[0026] Preferably, the pharmaceutical composition further comprises one or more pharmaceutically acceptable excipients.

[0027] Preferably, the pharmaceutical composition may further contain one or more additional therapeutic agents.

[0028] According to the third aspect of the present invention, the present invention also provides a method for preventing, treating or alleviating diseases associated with soluble epoxide hydrolases, comprising administering to a patient an effective amount of a compound represented by formula (I) or (II), or at least one of its stereoisomers, geometric isomers, tautomers, isotope labels, hydrates, solvates, polymorphs, metabolites, prodrugs, pharmaceutically acceptable salts or esters.

[0029] Preferably, the diseases associated with soluble epoxide hydrolase include NAFLD, fatty liver, steatohepatitis, liver fibrosis and cirrhosis.

[0030] Preferably, the patient is a mammal, preferably a human.

[0031] According to the fourth aspect of the present invention, the present invention also provides a compound represented by formula (I) or (II), and the use of at least one of its stereoisomers, geometric isomers, tautomers, isotope-labeled substances, hydrates, solvates, polymorphs, metabolites, prodrugs, pharmaceutically acceptable salts or esters in the preparation of drugs.

[0032] Preferably, the use may be use in preparing a drug for treating diseases associated with soluble epoxide hydrolase.

[0033] Preferably, the use may be for preparing a drug for treating, preventing or alleviating NAFLD, fatty liver, steatohepatitis, liver fibrosis and cirrhosis.

[0034] Beneficial Effects

[0035] The present invention provides a class of sEH inhibitors with a novel structure, enriching the structural types of sEH inhibitors and making up for the inherent deficiencies of urea-containing sEH inhibitors due to the presence of urea groups in the molecular structure. According to the present invention, a compound having an N-substituted 3,4-dihydroisoquinoline-1(2H)-one structure as a molecular skeleton can effectively improve the inhibitory activity of human soluble epoxide hydrolase and improve the therapeutic index confirmed by an animal model of non-alcoholic fatty liver disease, and also has good drug properties.

[0036] Definitions and explanations of terms

[0037] Unless otherwise specified, the definitions of groups and terms recorded in the specification and claims of this application, including their definitions as examples, exemplary definitions, preferred definitions, definitions recorded in tables, definitions of specific compounds in examples, etc., can be arbitrarily combined and combined with each other. The group definitions and compound structures after such combination and combination shall fall within the scope recorded in the specification of this application.

[0038] Unless otherwise specified, the numerical ranges recorded in this specification and claims are equivalent to recording at least each specific integer value therein. For example, the numerical range "1-40" is equivalent to recording each integer value in the numerical range "1-10", namely 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, and each integer value in the numerical range "11-40", namely 11, 12, 13, 14, 15, ..., 35, 36, 37, 38, 39, 40. It should be understood that in the one, two or more used herein when describing substituents, "more" should refer to an integer ≥ 3, such as 3, 4, 5, 6, 7, 8, 9 or 10.

[0039] The term "halogen" denotes a substituent group selected from fluorine, chlorine, bromine or iodine.

[0040] The term "C 1-12 "Alkyl" is understood to mean preferably a straight-chain or branched saturated monovalent hydrocarbon group having 1 to 12 carbon atoms. For example, "C 1-6 The term "alkyl" refers to straight-chain and branched alkyl groups having 1, 2, 3, 4, 5 or 6 carbon atoms. The alkyl group is, for example, methyl, ethyl, propyl, butyl, pentyl, hexyl, isopropyl, isobutyl, sec-butyl, tert-butyl, isopentyl, 2-methylbutyl, 1-methylbutyl, 1-ethylpropyl, 1,2-dimethylpropyl, neopentyl, 1,1-dimethylpropyl, 4-methylpentyl, 3-methylpentyl, 2-methylpentyl, 1-methylpentyl, 2-ethylbutyl, 1-ethylbutyl, 3,3-dimethylbutyl, 2,2-dimethylbutyl, 1,1-dimethylbutyl, 2,3-dimethylbutyl, 1,3-dimethylbutyl or 1,2-dimethylbutyl, or the like or isomers thereof.

[0041] The term "C 2-12 "Alkenyl" is understood to mean preferably a linear or branched monovalent hydrocarbon radical which contains one or more double bonds and has 2 to 12 carbon atoms. 2-12 "Alkenyl" is understood to mean preferably a linear or branched monovalent hydrocarbon radical comprising one or more double bonds and having 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12 carbon atoms. It is understood that in case the alkenyl contains more than one double bond, the double bonds may be separated from one another or conjugated.

[0042] The term "C 2-12 "Alkynyl" is understood to mean a linear or branched monovalent hydrocarbon radical containing one or more triple bonds and having 2 to 12 carbon atoms. 2-12 "Alkynyl" is understood as preferably meaning a straight-chain or branched, monovalent hydrocarbon radical which contains one or more triple bonds and has 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12 carbon atoms.

[0043] The term "C 3-6 "Cycloalkyl" is understood to mean a saturated monovalent monocyclic, bicyclic hydrocarbon ring or bridged cycloalkane having 3 to 6 carbon atoms. 3-6 "Cycloalkyl" is understood to mean a saturated monovalent monocyclic, bicyclic hydrocarbon ring or a bridged cycloalkane having 3, 4, 5 or 6 carbon atoms. 3-6 The cycloalkyl group may be a monocyclic hydrocarbon group such as cyclopropyl, cyclobutyl, cyclopentyl or cyclohexyl.

[0044] The term "C 6-14 "Aryl" is understood to mean preferably a monovalent aromatic or partially aromatic monocyclic, bicyclic or tricyclic hydrocarbon ring having 6 to 14 carbon atoms. 6-14 The term "aryl" is understood to mean preferably a monovalent aromatic or partially aromatic monocyclic, bicyclic or tricyclic hydrocarbon ring ("C 6-14 or a ring having 9 carbon atoms ("C9 aryl"), for example indanyl or indenyl, or a ring having 10 carbon atoms ("C 10 aryl), such as tetrahydronaphthyl, dihydronaphthyl or naphthyl, or a ring having 13 carbon atoms ("C 13 aryl), such as fluorenyl, or a ring having 14 carbon atoms ("C 14 aryl”), such as anthracenyl. When the C 6-20 When the aryl group is substituted, it may be monosubstituted or polysubstituted. Also, there is no limitation on the substitution position, for example, it may be substituted at the ortho position, para position or meta position.

[0045] The term "5-14 membered heteroaryl" is to be understood as including monovalent mono-, bi- or tricyclic aromatic ring systems having 5, 6, 7, 8, 9, 10, 11, 12, 13 or 14 ring atoms, in particular 5 or 6 or 9 or 10 carbon atoms, and which contain 1 to 5, preferably 1 to 3 heteroatoms which are each independently selected from N, O and S and which, in addition, in each case may be benzo-fused. In particular, the heteroaryl group is selected from thienyl, furanyl, pyrrolyl, oxazolyl, thiazolyl, imidazolyl, pyrazolyl, isoxazolyl, isothiazolyl, oxadiazolyl, triazolyl, thiadiazolyl, thia-4H-pyrazolyl and the like and benzo derivatives thereof, such as benzofuranyl, benzothienyl, benzoxazolyl, benzisoxazolyl, benzimidazolyl, benzotriazolyl, indazolyl, indolyl, isoindolyl and the like; or pyridyl, pyridazinyl, pyrimidinyl, pyrazinyl, triazinyl and the like and benzo derivatives thereof, such as quinolyl, quinazolinyl, isoquinolyl and the like; or azinyl, indolizinyl, purinyl and the like and benzo derivatives thereof; or cinnolinyl, phthalazinyl, quinazolinyl, quinoxalinyl, naphthyridinyl, pteridinyl, carbazolyl, acridinyl, phenazinyl, phenothiazinyl, phenoxazinyl and the like. When the 5-14 membered heteroaryl is connected to other groups to form the compound of the present invention, the carbon atom on the 5-14 membered heteroaryl ring may be connected to other groups, or the heteroatom on the 5-14 membered heteroaryl ring may be connected to other groups. When the 5-14 membered heteroaryl is substituted, it may be monosubstituted or polysubstituted. In addition, there is no limitation on the substitution site, for example, the hydrogen connected to the carbon atom on the heteroaryl ring may be substituted, or the hydrogen connected to the heteroatom on the heteroaryl ring may be substituted.

[0046] Unless otherwise specified, heterocyclic groups, heteroaryls or heteroarylene groups include all possible isomeric forms thereof, such as positional isomers thereof. Thus, for some illustrative non-limiting examples, forms substituted or bonded to other groups at one, two or more positions of the 1-, 2-, 3-, 4-, 5-, 6-, 7-, 8-, 9-, 10-, 11-, 12-positions, etc. (if present), may include pyridin-2-yl, pyridin-2-ylene, pyridin-3-yl, pyridin-3-ylene, pyridin-4-ylene and pyridin-4-ylene; thienyl or thienylene groups include thien-2-yl, thien-2-ylene, thien-3-ylene and thien-3-ylene; pyrazol-1-yl, pyrazol-3-yl, pyrazol-4-yl, pyrazol-5-yl.

[0047] The term "oxo" refers to a substituent in which a carbon atom, a nitrogen atom or a sulfur atom is oxidized to form an oxy group (=O).

[0048] Unless otherwise indicated, the definitions of terms herein also apply to groups containing the structures defined by the terms, for example, C 1-6 The definition of alkyl also applies to C 1-6Alkoxy, -N(C 1-6 Alkyl)2, -NHC 1-6 Alkyl or -S(O)2-C 1-6 Alkyl, etc.

[0049] It will be appreciated by those skilled in the art that the compounds of formula (I) may exist in the form of various pharmaceutically acceptable salts. If these compounds have a basic center, they may form acid addition salts; if these compounds have an acidic center, they may form a base addition salt; if these compounds contain both an acidic center (e.g., a carboxyl group) and a basic center (e.g., an amino group), they may also form an inner salt.

[0050] The compounds of the present invention may exist in the form of solvates (e.g. hydrates), wherein the compounds of the present invention contain polar solvents as structural elements of the crystal lattice of the compounds, in particular water, methanol or ethanol. The amount of polar solvents, in particular water, may exist in a stoichiometric or non-stoichiometric ratio.

[0051] According to their molecular structure, the compounds of the present invention may be chiral and therefore may exist in various enantiomeric forms. These compounds may thus exist in racemic form or in optically active form. The compounds of the present invention or their intermediates may be separated into enantiomeric compounds by chemical or physical methods known to those skilled in the art, or used in this form for synthesis. In the case of racemic amines, diastereomers are prepared from the mixture by reaction with optically active resolution agents. Examples of suitable resolution agents are optically active acids, such as tartaric acid, diacetyltartaric acid, dibenzoyltartaric acid, mandelic acid, malic acid, lactic acid, suitable N-protected amino acids (e.g. N-benzoylproline or N-phenylsulfonylproline) or various optically active camphorsulfonic acids in the R and S forms. Chromatographic enantiomer resolution may also be advantageously performed with the aid of optically active resolution agents (e.g. dinitrobenzoylphenylglycine, cellulose triacetate or other carbohydrate derivatives or chiral derivatized methacrylate polymers immobilized on silica gel). Suitable eluents for this purpose are aqueous or alcoholic solvent mixtures, for example hexane / isopropanol / acetonitrile.

[0052] The term "tautomer" refers to functional group isomers resulting from the rapid movement of an atom in a molecule between two positions. The compounds of the present invention may exhibit tautomerism. Tautomeric compounds may exist in two or more interconvertible species. Prototropic tautomers arise from the migration of a covalently bonded hydrogen atom between two atoms. Tautomers generally exist in equilibrium, and attempts to separate a single tautomer usually produce a mixture whose physicochemical properties are consistent with a mixture of compounds. The position of equilibrium depends on the chemical characteristics within the molecule. For example, in many aliphatic aldehydes and ketones such as acetaldehyde, the keto form predominates; while in phenols, the enol form predominates. The present invention encompasses all tautomeric forms of the compounds.

[0053] The term "geometric isomer" refers to the isomers produced when the two carbon atoms constituting the double bond are connected to different atoms or groups because the carbon-carbon double bond in the molecule cannot rotate freely, such as cis- and trans-isomers.

[0054] The corresponding stable isomers can be separated according to known methods, for example by extraction, filtration or column chromatography. The term "patient" refers to any animal including mammals, preferably mice, rats, other rodents, rabbits, dogs, cats, pigs, cows, sheep, horses or primates, most preferably humans.

[0055] The term "therapeutically effective amount" refers to the amount of an active compound or drug that elicits the biological or medical response that a researcher, veterinarian, physician or other clinician is seeking in a tissue, system, animal, individual or human, and includes one or more of the following: (1) Preventing disease: e.g., preventing a disease, disorder or condition in an individual who is susceptible to the disease, disorder or condition but does not yet experience or develop the pathology or symptoms of the disease. (2) Treating disease: e.g., inhibiting the disease, disorder or condition (i.e., preventing further development of the pathology and / or symptoms) in an individual who is experiencing or developing the pathology or symptoms of the disease, disorder or condition. (3) Alleviating disease: e.g., alleviating the disease, disorder or condition (i.e., reversing the pathology and / or symptoms) in an individual who is experiencing or developing the pathology or symptoms of the disease, disorder or condition. BRIEF DESCRIPTION OF THE DRAWINGS

[0056] Figure 1 and Figure 2 The photographs of the analysis of the pathological changes and fat accumulation degree of liver tissues in each group in Biological Example 2 are shown (HE staining and Oil Red O staining, ×200). Example

[0057] The following examples are used to illustrate the present invention but are not intended to limit the scope of the present invention.

[0058] Example 1: 6,8-dimethoxy-3,4-dihydroisoquinolin-1-(2H)-one

[0059]

[0060] 1.0 g (4.7 mmol) of 3,5-dimethoxybenzenepropionic acid was added to 2.0 mL of a mixed solution of water and acetone, and the temperature was lowered to 0°C. 1.0 mL of an acetone solution containing 0.7 mL (5.0 mmol) of triethylamine was added thereto, and then 1.0 mL of an acetone solution containing 0.6 mL (6.3 mmol) of ethyl chloroformate was added thereto, and the temperature was controlled at 0°C for reaction for 0.5 h. 1.0 mL of an aqueous solution containing 0.5 g (7.7 mmol) of sodium azide was added thereto, and the temperature was controlled at 0°C for reaction for 1 h. The reaction solution was poured into ice water, extracted with ethyl acetate (20 mL × 3), the organic phase was separated and dried over anhydrous magnesium sulfate, and concentrated under reduced pressure. 2.0 mL of toluene was added thereto, and the reaction was heated at 110°C for 2 h. The reaction solution was concentrated under reduced pressure, and 0.5 mL of trifluoromethanesulfonic acid was added dropwise at 0°C. After the addition was completed, the temperature was raised to 100°C for 2 h. The reaction solution was poured into a mixture of 20 mL of ethyl acetate and 10 mL of ice water. The organic layer was separated and washed with water, dried over anhydrous magnesium sulfate, and concentrated under reduced pressure. The residue was subjected to column chromatography to obtain 0.6 g of an off-white solid with a yield of 68.1%. 1 H NMR (500 MHz, CDCl 3, ppm)δ:7.07(s,1H),6.40(d,J=2.2Hz,1H),6.32(d,J=2.1Hz,1H),3.90(s,3H),3.8 4(s,3H),3.43(td,J=6.5,2.8Hz,2H),2.89(t,J=6.4Hz,2H); HRMS(ESI):m / z[M+H] + :208.0969.

[0061] Example 2: 2-(3-fluorobenzyl)-6,8-dimethoxy-3,4-dihydroisoquinolin-1-(2H)-one (Compound 1)

[0062]

[0063] 30 mg (0.1 mmol) of 6,8-dimethoxy-3,4-dihydroisoquinolin-1-(2H)-one was added to 1.0 mL of DMF, and 8 mg (0.2 mmol) of sodium hydride and 27 mg (0.1 mmol) of 3-fluorobenzyl bromide were added thereto under ice bath. The reaction was carried out at 0°C for 3 h, and water was added to quench the reaction. The product was extracted with ethyl acetate (10 mL×3), and the organic phase was separated and dried over anhydrous sodium sulfate, and concentrated under reduced pressure. The residue was subjected to column chromatography to obtain 24 mg of a light yellow oil with a yield of 53.3%.1 H NMR (500 MHz, CDCl 3, ppm)δ:7.31-7.26(m,1H),7.12(d,=7.6Hz,1H),7.06(d,J=9.6Hz,1H),6.95(d,J=2.2Hz,1H),6.42(d,J=2.3Hz,1H),6.26 (d,J=2.3Hz,1H),4.74(s,2H),3.92(s,3H),3.83(s,3H),3.43-3.38(m,2H),2.82(t,J=6.4Hz,2H); HRMS(ESI):m / z[M+H] + :316.1339.

[0064] Example 3: 2-(3-fluorobenzyl)-8-hydroxy-6-methoxy-3,4-dihydroisoquinolin-1-(2H)-one (Compound 2)

[0065]

[0066] 333 mg (1.1 mmol) of compound 1 was added to 2.0 mL of N,N-dimethylacetamide, to which 448 mg (10.6 mmol) of lithium chloride was added, and the mixture was reacted at 140 °C for 6 h under nitrogen protection. Water was added to quench the reaction, and the mixture was extracted with ethyl acetate (15 mL×3). The organic phase was dried over anhydrous sodium sulfate and concentrated under reduced pressure. The residue was subjected to column chromatography to obtain 180 mg of a light yellow oil with a yield of 56.6%. 1 H NMR (500 MHz, CDCl 3, ppm)δ:7.31(td,J=7.9,6.0Hz,1H),7.10(d,J=7.6Hz,1H),7.00(ddd,J=12.2,10.6,5.9Hz,2H),6.34(d,J=2.3Hz,1H) ,6.19(d,J=2.1Hz,1H),4.71(s,2H),3.80(s,3H),3.45(t,J=6.8Hz,2H),2.89(t,J=6.7Hz,2H); HRMS(ESI):m / z[M+H] + :302.1195.

[0067] Example 4: 2-(3-fluorobenzyl)-6-methoxy-8-isopentenyloxy-3,4-dihydroisoquinolin-1-(2H)-one (Compound 3)

[0068]

[0069] 142 mg (0.5 mmol) of compound 2 was added into 2.0 mL of DMF, to which 28 mg (0.7 mmol) of sodium hydride was added, and then 0.06 mL (0.5 mmol) of isopentenyl bromide was added. The reaction was allowed to react at room temperature for 2 h. Water was added to quench the reaction, and the mixture was extracted with ethyl acetate (15 mL×3). The organic phases were combined and washed with water and saturated sodium chloride three times each, dried over anhydrous sodium sulfate, and concentrated under reduced pressure. The residue was subjected to column chromatography to obtain 152 mg of a light yellow oil in a yield of 87.3%. 1 H NMR (500 MHz, CDCl 3, ppm)δ:7.27(dt,J=7.9,7.0Hz,1H),7.11(d,J=7.6Hz,1H),7.05(d,J=9.7Hz,1H) ,6.94(td,J=8.4,2.0Hz,1H),6.42(d,J=2.2Hz,1H),6.25(d,J=2.1Hz,1H),5.57 (t,J=6.4Hz,1H),4.74(s,2H),4.66(d,J=6.4Hz,2H),3.82(s,3H),3.39(t,J=6. 4Hz,2H),2.80(t,J=6.3Hz,2H),1.77(s,3H),1.75(s,3H); HRMS(ESI):m / z[M+H] + :370.1811.

[0070] Example 5: 2-(3-fluorobenzyl)-8-hydroxy-6-methoxy-5-isopentenyl-3,4-dihydroisoquinolin-1-(2H)-one (Compound 4)

[0071]

[0072] 0.48 g (1.3 mmol) of compound 3 was added to 6.0 mL of anhydrous dichloromethane, and montmorillonite K was added thereto in batches. 10 3.0 g, after addition, react for 4 h, filter and concentrate under reduced pressure, and purify the residue by column chromatography to obtain 145 mg of light yellow oil, with a yield of 30.0%. 1 H NMR (500 MHz, CDCl 3,ppm)δ:7.34(m,1H),7.12(d,J=2.0Hz,1H),7.06(m,2H),6.45(s,1H),5.37(t,J=6.4Hz,1H),4.76(s,2H),3.84(t,J=6 .4Hz,2H),3.82(s,3H),3.56(d,J=6.4Hz,2H),3.39(t,J=6.4Hz,2H),1.79(s,3H),1.76(s,3H); HRMS(ESI):m / z[M+H] + :370.1810.

[0073] Example 6: 6,8-dimethoxy-2-(1-phenylethyl)-3,4-dihydroisoquinolin-1-(2H)-one (Compound 5)

[0074]

[0075] The raw material was replaced by (1-bromoethyl)benzene from 3-fluorobenzyl bromide, and the preparation was carried out according to the method of Example 2 with a yield of 92.3%. 1 H NMR (500 MHz, CDCl 3, ppm)δ:7.40(d,J=7.3Hz,2H),7.32-7.29(m,2H),7.28-7.23(m,1H),6.42(d,J=2.3Hz,1H),6.28(q,J=7.1Hz,1H),6.22(d, J=2.3Hz,1H),3.93(s,3H),3.82(s,3H),3.29-3.00(m,2H),2.72-2.60(m,2H),1.55(d,J=7.1Hz,3H); HRMS(ESI):m / z[M+H] + :312.1607).

[0076] Example 7: 6,8-dimethoxy-2-[1-(3-trifluoromethyl-phenyl)ethyl]-3,4-dihydroisoquinolin-1-(2H)-one (Compound 6)

[0077]

[0078] The raw material was replaced by 3-(1-bromoethyl)trifluorotoluene from 3-fluorobenzyl bromide, and the preparation was carried out according to the method of Example 2 with a yield of 72.1%. 1H NMR (500MHz, CDCl3, ppm) δ: 7.65-7.58 (m, 2H), 7.53 (d, J = 7.7Hz, 1H), 7.45 (t, J = 7.8Hz, 1H), 6.43 (d, J = 2.3Hz, 1H), 6.32 (q, J = 7.1Hz, 1 H),6.24(d,J=2.3Hz,1H),3.94(s,3H),3.83(s,3H),3.32-2.97(m,2H),2.73-2.64(m,2H),1.58(d,J=7.1Hz,3H); HRMS(ESI):m / z[M+H] + :380.1473.

[0079] Example 8: 6,8-dimethoxy-2-(3-trifluoromethyl-benzyl)-3,4-dihydroisoquinolin-1-(2H)-one (Compound 7)

[0080]

[0081] The raw material was replaced by 3-(trifluoromethyl)benzyl bromide from 3-fluorobenzyl bromide, and the preparation was carried out according to the method of Example 2 with a yield of 47.2%. 1 H NMR (500 MHz, CDCl 3, ppm)δ:7.59-7.50(m,3H),7.44(t,J=7.6Hz,1H),6.43(d,J=2.3Hz,1H),6.27(d,J=2.3Hz,1H),4.8 0(s,2H),3.93(s,3H),3.84(s,3H),3.44-3.38(m,2H),2.82(t,J=6.4Hz,2H); HRMS(ESI):m / z[M+H] + :366.1308.

[0082] Example 9: 2-(3-chloro-4-fluorobenzyl)-6,8-dimethoxy-3,4-dihydroisoquinolin-1-(2H)-one (Compound 8)

[0083]

[0084] The raw material was replaced by 3-chloro-4-fluorobenzyl bromide from 3-fluorobenzyl bromide, and the preparation was carried out according to the method of Example 2 with a yield of 45.4%. 1 H NMR (500 MHz, CDCl 3,ppm)δ:7.40(dd,J=7.0,2.1Hz,1H),7.25-7.20(m,1H),7.08(t,J=8.7Hz,1H),6.42(d,J=2.3Hz,1H),6.26(d,J=2 .3Hz,1H),4.67(s,2H),3.92(s,3H),3.83(s,3H),3.43-3.38(m,2H),2.82(t,J=6.4Hz,2H); HRMS(ESI):m / z[M+H] + :350.0948.

[0085] Example 10: 2-(3-nitro-5-trifluoromethylbenzyl)-6,8-dimethoxy-3,4-dihydroisoquinolin-1-(2H)-one (Compound 9)

[0086]

[0087] The raw material was replaced by 3-nitro-5-trifluoromethylbenzyl bromide, and the preparation was carried out according to the method of Example 2 with a yield of 45.4%. 1 H NMR (600MHz, CDCl3) δ: 8.38 (d, J = 3.5, 2H), 7.96 (s, 1H), 6.44 (d, J = 1.7, 1H), 6.30 (d, J = 1.3, 1H), 4. 88(s,2H),3.92(s,3H),3.85(s,3H),3.52(t,J=6.3,2H),2.91(t,J=6.2,2H); HRMS(ESI):m / z[M+H] + :411.1164.

[0088] Example 11: 8-Hydroxy-6-methoxy-2-(1-phenylethyl)-3,4-dihydroisoquinolin-1-(2H)-one (Compound 10)

[0089]

[0090] The raw material was replaced by compound 5 from compound 1 and prepared according to the method of Example 3 with a yield of 59.5%. 1 HNMR (500MHz, CDCl 3, ppm)δ:7.39-7.27(m,5H),6.34(d,J=2.4Hz,1H),6.16-6.10(m,2H),3.79(s,3H),3. 37-3.01(m,2H),2.74(t,J=6.2Hz,2H),1.59(d,J=7.1Hz,3H); HRMS(ESI):m / z[M+H] + :298.1448.

[0091] Example 12: 8-Hydroxy-6-methoxy-2-[1-(3-trifluoromethyl-phenyl)ethyl]-3,4-dihydroisoquinolin-1-(2H)-one (Compound 11)

[0092]

[0093] The raw material was replaced by compound 6 from compound 1 and the product was prepared according to the method of Example 3 with a yield of 97.0%. 1 HNMR (500MHz, CDCl 3, ppm)δ:7.62-7.45(m,4H),6.35(d,J=2.4Hz,1H),6.19-6.14(m,2H),3.80(s,3H),3. 41-2.98(m,2H),2.77(t,J=6.6Hz,2H),1.63(d,J=7.1Hz,3H); HRMS(ESI):m / z[M+H] + :366.1310.

[0094] Example 13: 8-Hydroxy-6-methoxy-2-(3-trifluoromethyl-benzyl)-3,4-dihydroisoquinolin-1-(2H)-one (Compound 12)

[0095]

[0096] The raw material was replaced by compound 7 from compound 1 and prepared according to the method of Example 3 with a yield of 77.9%. 1 HNMR (500MHz, CDCl 3, ppm)δ:7.58-7.45(m,4H),6.35(d,J=2.4Hz,1H),6.20-6.18(m,1H),4.78(s,2H) ,3.80(s,3H),3.45(t,J=6.8Hz,2H),2.89(t,J=6.7Hz,2H); HRMS(ESI):m / z[M+H] + :352.1166.

[0097] Example 14: 2-(3-chloro-4-fluorobenzyl)-8-hydroxy-6-methoxy-3,4-dihydroisoquinolin-1-(2H)-one (Compound 13)

[0098]

[0099] The raw material was replaced by compound 8 from compound 1 and the product was prepared according to the method of Example 3 with a yield of 58.3%. 1 HNMR (500MHz, CDCl 3,ppm)δ:7.41(dd,J=6.9,2.1Hz,1H),7.25(ddd,J=8.4,4.5,2.2Hz,1H),7.16(t,J=8.6Hz,1H),6.38(d,J=2.4Hz,1H ),6.24-6.21(m,1H),4.70(s,2H),3.84(s,3H),3.48(t,J=6.8Hz,2H),2.93(t,J=6.7Hz,2H); HRMS(ESI):m / z[M+H] + :336.0809.

[0100] Example 15: 2-[(3-nitro-5-trifluoromethyl)benzyl]-8-hydroxy-6-methoxy-3,4-dihydroisoquinolin-1-(2H)-one (Compound 14)

[0101]

[0102] The raw material was replaced by compound 9 from compound 1, and the product was prepared according to the method of Example 3 with a yield of 59.9%. 1 HNMR (400MHz, CDCl3) δ: 8.43 (s, 1H), 8.38 (s, 1H), 7.94 (s, 1H), 6.34 (d, J = 2.3, 1H), 6.24-6.12 ( m,1H),4.86(s,2H),3.81(s,3H),3.54(t,J=6.7,2H),2.96(t,J=6.7,2H); HRMS(ESI):m / z[M+H] + :397.1160.

[0103] Example 16: 6-methoxy-8-isopentenyloxy-2-(1-phenylethyl)-3,4-dihydroisoquinolin-1-(2H)-one (Compound 15)

[0104]

[0105] The product was prepared using compound 10 and isopentenyl bromide according to the method of Example 4 with a yield of 61.8%. 1 HNMR (500MHz, CDCl 3,ppm)δ:7.40(d,J=7.8Hz,2H),7.32(t,J=7.5Hz,2H),7.27-7.23(m,1H),6.41(d,J= 2.3Hz,1H),6.28(q,J=7.0Hz,1H),6.21(d,J=2.2Hz,1H),5.59(ddd,J=6.3,5.1,1. 3Hz,1H),4.68(d,J=6.3Hz,2H),3.80(s,3H),3.29-2.99(m,2H),2.64(dt,J=10.6, 5.2Hz,2H),1.78(s,3H),1.76(s,3H),1.54(d,J=7.1Hz,3H); HRMS(ESI):m / z[M+H] + :366.2065.

[0106] Example 17: 6-methoxy-8-isopentenyloxy-2-[1-(3-trifluoromethyl-phenyl)ethyl]-3,4-dihydroisoquinolin-1-(2H)-one (Compound 16)

[0107]

[0108] The product was prepared using compound 11 and isopentenyl bromide according to the method of Example 4 with a yield of 71.2%. 1 HNMR (500MHz, CDCl 3, ppm)δ:7.64-7.58(m,2H),7.52(d,J=7.7Hz,1H),7.44(t,J=7.7Hz,1H),6.42(d,J =2.3Hz,1H),6.33(q,J=7.0Hz,1H),6.22(d,J=2.3Hz,1H),5.59(ddd,J=6.4,5.1,1 .4Hz,1H),4.68(d,J=6.3Hz,2H),3.81(s,3H),3.32-2.96(m,2H),2.67(dd,J=7.4, 4.4Hz,2H),1.78(s,3H),1.76(s,3H),1.57(d,J=7.1Hz,3H); HRMS(ESI):m / z[M+H] + :434.1929.

[0109] Example 18: 6-methoxy-8-isopentenyloxy-2-[3-(trifluoromethyl)benzyl]-3,4-dihydroisoquinolin-1-(2H)-one (Compound 17)

[0110]

[0111] The compound 12 and isopentenyl bromide were used to prepare the product according to the method of Example 4 with a yield of 69.3%. 1 HNMR (400MHz, CDCl3) δ: 7.55 (m, 3H), 7.44 (d, J = 7.7, 1H), 6.43 (d, J = 2.3, 1H), 6.25 (d, J = 2.2, 1H), 5.57 (t, J = 6.4, 1H), 4.80 (s, 2H),4.66(d,J=6.4,2H),3.81(s,3H),3.40(t,J=6.4,2H),2.80(t,J=6.3,2H),1.77(s,3H),1.75(s,3H); HRMS(ESI):m / z[M+H] + :420.1770.

[0112] Example 19: 6-methoxy-8-isopentenyloxy-2-(3-chloro-4-fluorobenzyl)-3,4-dihydroisoquinolin-1-(2H)-one (Compound 18)

[0113]

[0114] The product was prepared using compound 13 and isopentenyl bromide according to the method of Example 4 with a yield of 71.8%. 1 HNMR (500MHz, CDCl 3, ppm)δ:7.40(dd,J=7.0,1.8Hz,1H),7.24-7.19(m,1H),7.07(t,J=8.7Hz,1H),6.42(d,J=2.1Hz,1H),6.25(d,J=1.8Hz,1H),5.57(t,J=6.3Hz,1H ),4.67(s,2H),4.66(d,J=6.5Hz,2H),3.82(s,3H),3.39(t,J=6.4Hz,2H),2.79(t,J=6.3Hz,2H),1.77(s,3H),1.75(s,3H); HRMS(ESI):m / z[M+H] + :404.1430.

[0115] Example 20: 6-methoxy-8-isopentenyloxy-2-[(3-nitro-5-trifluoromethyl)benzyl]-3,4-dihydroisoquinolin-1-(2H)-one (Compound 19)

[0116]

[0117] The product was prepared using compound 14 and isopentenyl bromide according to the method of Example 4 with a yield of 72.1%. 1HNMR (400MHz, CDCl3) δ: 8.39 (d, J = 3.2, 2H), 7.95 (s, 1H), 6.45 (d, J = 1.6, 1H), 6.31 (d, J = 1.6, 1H), 5.55 (t, J = 6.3Hz, 1H), 4.80 (s, 2H),4.66(d,J=6.5Hz,2H),3.85(s,3H),3.42(t,J=6.2,2H),2.90(t,J=6.2,2H),1.78(s,3H),1.75(s,3H); HRMS(ESI):m / z[M+H] + :465.1639.

[0118] Example 21: 6-methoxy-8-geranyloxy-2-(3-trifluoromethyl-benzyl)-3,4-dihydroisoquinolin-1-(2H)-one (Compound 20)

[0119]

[0120] The compound 12 and geranyl bromide were used to prepare the product according to the method of Example 4 with a yield of 55.3%. 1 HNMR (400MHz, CDCl3) δ: 7.73 (s 1H),7.57(d,J=7.5,1H),7.52(m,1H),7.38(d,J=7.4,1H),6.55(d,J=1.4,1 H),6.32(d,J=1.4,1H),5.45(t,J=6.2,1H),5.19(s,2H),4.70(d,J=6.2,2H ),4.35(t,J=6.1,1H),3.85(s,3H),3.79(m,2H),3.10(t,J=5.4,2H),1.97( t,J=7.7,2H),1.84(s,6H),1.75(s,3H),1.57(m,2H).;HRMS(ESI):m / z[M+H] + :488.2416.

[0121] Example 22: 2-(3-Trifluoromethyl-benzyl)-8-hydroxy-6-methoxy-5-geranyl-3,4-dihydroisoquinolin-1-(2H)-one (Compound 21)

[0122]

[0123] The product was prepared using compound 20 according to the method of Example 5 with a yield of 26.4%. 1H NMR(400MHz, CDCl3)δ:7.55(s,1H),7.52-7.44(m,2H),7.43-7.37(m,1H), 6.39(s,1H),5.60(t,J=6.2,1H),5.46–5.23(m,1H),4.80(s,2H),3.94(m,2 H),3.82(s,3H),3.46(d,J=6.1,2H),3.10(t,J=5.5,2H),2.17-2.09(m,2H) ,2.05(m,2H),1.91(s,3H),1.82(s,3H),1.78(s,3H); HRMS(ESI):m / z[M+H] + :488.2418.

[0124] Example 23: 8-[2-(5-ethylpyridin-2-yl)ethoxy]-6-methoxy-2-(3-trifluoromethyl-benzyl)-3,4-dihydroisoquinolin-1(2H)-one (Compound 22)

[0125]

[0126] Compound 12 and 2-(5-ethyl-2-pyridyl)ethanol p-toluenesulfonate were used to prepare the product according to the method of Example 4 with a yield of 34.5%. 1 H NMR (600MHz, CDCl3) δ: 8.37 (s, 1H), 7.59 (s, 1H), 7.54 (t, J = 8.1, 2H), 7.48 (s, 2H), 7.45 (t, J = 7.7, 1H), 6.45 (d, J = 1.6, 1H), 6.26 (s, 1H), 4.82 ( s,2H),4.41(t,J=6.9,2H),3.80(s,3H),3.40(t,J=5.8,4H),2.81(t,J= 6.3,2H),2.63(q,J=7.6,2H),1.24(t,J=7.6,3H); HRMS(ESI):m / z[M+H] + :485.2036.

[0127] Example 24: 6-methoxy-8-[2-(5-methyl-2-phenyloxazol-4-yl)ethoxy]-2-(3-trifluoromethyl-benzyl)-3,4-dihydroisoquinolin-1(2H)-one (Compound 23)

[0128]

[0129] Compound 12 and 2-(5-methyl-2-phenyl-4-oxazolyl)ethanol p-toluenesulfonate were used to prepare the product according to the method of Example 4 with a yield of 64.5%.1 H NMR(400MHz, CDCl3)δ:7.98(dd,J=7.9,1.7,2H),7.58(s,1H),7.53(t,J=6.7,2H),7.48–7.35(m,4H),6.47(d,J=2.3,1H),6.25(d,J=2.2,1H), 4.80(s,2H),4.34(t,J=7.0,2H),3.78(s,3H),3.39(t,J=6.3,2H),3.1 2(t,J=7.0,2H),2.80(t,J=6.3,2H),2.40(s,3H); HRMS(ESI):m / z[M+H] + :537.2026.

[0130] Example 25: 8-{2-[Benzo[d]oxazol-2-yl(methylamino)]ethoxy}-6-methoxy-2-(3-trifluoromethyl-benzyl)-3,4-dihydroisoquinolin-1(2H)-one (Compound 24)

[0131]

[0132] Compound 12 and 2-(N-methyl-N-benzoxazole-2-amino)ethanol p-toluenesulfonate were used to prepare the product according to the method of Example 4 with a yield of 67.6%. 1 H NMR (600MHz, CDCl3) δ: 7.57 (s, 1H), 7.52 (t, J = 8.1, 2H), 7.44 (t, J = 7.7, 1H), 7.36 –7.31(m,1H),7.25(d,J=7.8,1H),7.14(td,J=7.7,1.0,1H),6.98(td,J=7.8,1.1, 1H),6.42(d,J=2.3,1H),6.27(d,J=2.2,1H),4.77(s,2H),4.34(t,J=5.2,2H),4. 05(t,J=5.2,2H),3.78(s,3H),3.47(s,3H),3.42–3.36(m,2H),2.81(t,J=6.3,2H) . ; HRMS (ESI): m / z [M+H] + :526.1964.

[0133] Example 26: 8-{2-[Benzo[d]thiazol-2-yl(methylamino)]ethoxy}-6-methoxy-2-(3-(trifluoromethyl)benzyl)-3,4-dihydroisoquinolin-1(2H)-one (Compound 25)

[0134]

[0135] Compound 12 and 2-(N-methyl-N-benzothiazole-2-amino)ethanol p-toluenesulfonate were used to prepare the product according to the method of Example 4 with a yield of 65.3%. 1 H NMR (600MHz, CDCl3) δ: 7.55 (dt, J = 16.2, 7.5, 5H), 7.44 (t, J = 7.7, 1H), 7.31–7.26 (m, 1H), 7.07–7.00 (m, 1H), 6.43 (d, J = 2.2, 1H), 6.26 (d, J = 2.1, 1H),4.79(s,2H),4.36(t,J=5.1,2H),4.12(t,J=5.1,2H),3.76(s,3H),3 .41(s,3H),3.40–3.36(m,2H),2.81(t,J=6.3,2H); HRMS(ESI):m / z[M+H] + :542.1734.

[0136] Example 27: 2-{4-[6-methoxy-1-oxo-2-(3-trifluoromethyl-benzyl)-1,2,3,4-tetrahydroisoquinolin-8-oxy-methylene]phenoxy}-2-methylpropanoic acid (Compound 26)

[0137]

[0138] 151 mg (0.5 mmol) of ethyl 2-[4-(bromomethyl)benzyloxy]-2-methylpropanoate, 176 mg (0.5 mmol) of compound 12, and 325 mg (1 mmol) of cesium carbonate were put into a reaction flask, 2 mL of dry DMF was added thereto, the reaction was allowed to proceed at 25°C overnight, 25 mL of water was added, the mixture was extracted with 30 mL of ethyl acetate, the organic phase was separated and washed with 25 mL of saturated sodium chloride, dried over anhydrous magnesium sulfate, and filtered to remove insoluble matter, evaporated to dryness, 5 mL of methanol and 2 mL of 10% sodium hydroxide were added, the mixture was refluxed at 80°C for 1 h, methanol was evaporated under reduced pressure, 15 mL of water was added to dissolve the mixture, the pH was adjusted to 1-2 with concentrated hydrochloric acid in an ice-water bath, 25 mL of ethyl acetate was added to extract the mixture, the ethyl acetate layer was separated, the mixture was washed with 15 mL of saturated brine, dried over anhydrous magnesium sulfate, and evaporated to dryness. The residue was subjected to column chromatography to obtain 0.17 g of the title compound in a yield of 62.6%. 1H NMR(600MHz, CDCl3)δ:7.57(d,J=12.9,2H),7.53(d,J=7.6,1H),7.48–7.38(m,3H),6.91(d,J=7.7,2H),6.41(s,1H),6.26 (s,1H),5.09(s,2H),4.83(s,2H),3.77(s,3H),3.40(t,J=5.8,2H),2.81(t,J=5.7,2H),1.57(s,6H); HRMS(ESI):m / z[M+H] + :544.1946.

[0139] Example 28: 4'-[6-methoxy-1-oxo-2-(3-trifluoromethyl-benzyl)-1,2,3,4-tetrahydroisoquinolin-8-oxy-methylene]-[1,1'-biphenyl]-3-carboxylic acid (Compound 27)

[0140]

[0141] The reaction was carried out according to the method of Example 27, except that 2-[4-(bromomethyl)benzyloxy]-2-methylpropionic acid ethyl ester was replaced with 4'-bromomethyl-3-methyl biphenylcarboxylate. The yield was 60.3%. 1 H NMR (400MHz, DMSO) δ: 13.09 (s, 1H), 8.22 (s, 1H), 7.95 (t, J = 6.8, 2H), 7.70 (m, 5H), 7.62 (m, 4H), 6.64 (d, J = 2.2, 1H), 6. 48(d,J=1.9,1H),5.25(s,2H),4.78(s,2H),3.81(s,3H),3.45(t,J=6.1,2H),2.85(t,J=6.0,2H); HRMS(ESI):m / z[M+H] + :562.1876.

[0142] Example 29: 4'-[6-methoxy-1-oxo-2-(3-trifluoromethyl-benzyl)-1,2,3,4-tetrahydroisoquinolin-8-oxy-methylene]-[1,1'-biphenyl]-2-carboxylic acid (Compound 28)

[0143]

[0144] The reaction was carried out according to the method of Example 27, except that 2-(4-bromomethyl-benzyloxy)-2-methylpropionic acid ethyl ester was replaced with 4'-bromomethyl-2-methylformate biphenyl. The yield was 60.8%. 1H NMR(600MHz, CDCl3)δ:7.89(dd,J=7.8,1.0,1H),7.58(d,J=10.2,1H),7.49(ddd,J=29.6,19.3,8.3,2H),7.40–7.34(m,1H),7.32(d,J=7.6,0H) ,6.40(d,J=2.2,0H),6.24(d,J=2.1,0H),5.05(s,1H),4.83(s,1H),3.7 3(s,3H),3.39(t,J=6.3,1H),2.80(t,J=6.3,1H); HRMS(ESI):m / z[M+H] + :562.1912.

[0145] Example 30: 2-[6-methoxy-1-oxo-2-(3-trifluoromethyl-benzyl)-1,2,3,4-tetrahydroisoquinolin-8-oxy]-2-methylpropanoic acid (Compound 29)

[0146]

[0147] The product was prepared according to the method of Example 27, except that 2-[4-(bromomethyl)benzyloxy]-2-methylpropanoic acid ethyl ester was replaced with 2-bromo-2-methylpropanoic acid ethyl ester. The yield was 54.7%. 1 H NMR (600MHz, CDCl3) δ: 7.49 (d, J = 1.9, 1H), 7.47 (m, 2H), 7.23 (d, J = 7.6, 1H), 6.43 (s, 1H), 6.28 (s, 1H) ,4.86(s,2H),3.48(s,3H),3.42(t,J=5.8,2H),2.81(t,J=5.7,2H),1.57(s,6H); HRMS(ESI):m / z[M+H] + :438.1529.

[0148] Example 31: 8-[2-(5-ethylpyridin-2-yl)ethoxy]-6-methoxy-2-[(3-trifluoromethyl-5-nitro-benzyl)]-3,4-dihydroisoquinolin-1(2H)-one (Compound 30)

[0149]

[0150] Compound 14 and 2-(5-ethyl-2-pyridyl)ethanol p-toluenesulfonate were used to prepare the product according to the method of Example 4 with a yield of 59.2%. 1H NMR (600MHz, CDCl3) δ: 8.48 (d, J = 1.4, 1H), 8.34–8.24 (m, 2H), 8.09 (d, J = 1.4, 1H), 7.49(dd,J=7.5,1.4,1H),7.12(d,J=7.3,1H),6.79(d,J=1.4,1H),6.68(d,J=1.6,1 H),4.87(s,2H),4.37(t,J=4.4,2H),3.90(m,2H),3.86(s,3H),3.32(t,J=4.4,2H) ,2.90(t,J=5.4,2H),2.65(q,J=6.6,2H),1.33(t,J=6.6,3H); HRMS(ESI):m / z[M+H] + :530.1906.

[0151] Example 32: 8-[2-(5-ethylpyridin-2-yl)ethoxy]-6-methoxy-2-[(3-trifluoromethyl-5-amino-)benzyl)]-3,4-dihydroisoquinolin-1(2H)-one (Compound 31)

[0152]

[0153] 1 g (1.9 mmol) of compound 30, 0.2 g of 10% palladium on carbon, 1 g of ammonium formate and 20 ml of methanol were put into a reaction bottle and catalytic hydrogenation reaction was carried out overnight. The insoluble matter was removed by filtration and the methanol was evaporated under reduced pressure. 20 ml of dichloromethane was added to the residue for slurrying and filtered to obtain a yellow solution. The filter cake was washed with 10 ml*2 of dichloromethane. The filtrates were combined and evaporated to dryness to obtain 0.9 g of the title compound in a yield of 95.0%. 1 H NMR (600MHz, CDCl3) δ: = 8.37 (d, J = 1.8, 1H), 7.46 (dd, J = 7.9, 2.3, 1H), 7.40 (d, J = 7.9,1H),6.91(s,1H),6.85(s,1H),6.79(s,1H),6.45(d,J=2.3,1H),6.25(d,J=2. 3,1H),4.69(s,2H),4.40(t,J=7.0,2H),3.79(s,3H),3.38(dd,J=12.5,6.2,4H), 2.79(t,J=6.3,2H),2.62(q,J=7.6,2H),1.23(t,J=7.6,3H); HRMS(ESI):m / z[M+H] + :500.2167.

[0154] Example 33: 8-[2-(5-ethylpyridin-2-yl)ethoxy]-6-methoxy-2-{[3-trifluoromethyl-5-(2,2-difluoro-2-chloroacetamido)]benzyl}-3,4-dihydroisoquinolin-1(2H)-one (Compound 32)

[0155]

[0156] 244 mg (0.49 mmol) of compound 31, 155 mg of EDC.HCl, 22 mg of DMAP and 10 mL of anhydrous DCM were put into a reaction flask and stirred under ice-water bath. 0.1 ml of difluorochloroacetic acid and 0.5 ml of DIPEA were added and reacted for 1 h. The mixture was washed with equal volumes of water, saturated sodium bicarbonate and brine in sequence and dried over anhydrous magnesium sulfate. The solvent was evaporated and the residue was purified by silica gel column to obtain 155 mg of the title compound in a yield of 51.9%. 1 H NMR (400MHz, CDCl3) δ = 10.13 (s, 1H), 8.31 (d, J = 1.8, 1H), 8.02 (s, 1H), 7.76 (s, 1H), 7.35 (dd,J=7.9,2.1,1H),7.28(s,1H),7.20(d,J=7.9,1H),6.42(d,J=2.3,1H),6.18(d,J=2. 1,1H),4.69(s,2H),4.35(t,J=6.8,2H),3.80(s,3H),3.42(t,J=6.4,2H),3.22(t,J=6.7 ,2H),2.80(t,J=6.3,2H),2.56(q,J=7.6,2H),1.18(t,J=7.6,3H); HRMS(ESI):m / z[M+H] + :612.1687.

[0157] Example 34: 8-[2-(5-ethylpyridin-2-yl)ethoxy]-6-methoxy-2-{[3-trifluoromethyl-5-(2-cyanoacetamido)]benzyl}-3,4-dihydroisoquinolin-1(2H)-one (Compound 33)

[0158]

[0159] Substituting difluorochloroacetic acid with cyanoacetic acid, the product was prepared according to the method of Example 33 with a yield of 33.5%. 1HNMR (400MHz, CDCl3) δ = 9.92 (s, 1H), 8.33 (d, J = 1.8, 1H), 7.77 (s, 1H), 7.53 (s, 1H), 7.39 (d d,J=7.9,1.6,1H),7.23(d,J=7.9,1H),7.11(s,1H),6.47(d,J=2.2,1H),6.28(d,J=2.1,1H) ,4.65(s,2H),4.41(t,J=6.6,2H),3.83(s,3H),3.49(t,J=6.4,2H),3.36(s,2H),3.26(t,J= 6.6,2H),2.89(t,J=6.3,2H),2.53(q,J=7.6,2H),1.14(t,J=7.6,3H); HRMS(ESI):m / z[M+H] + :567.2218.

[0160] Example 35: 8-[2-(5-ethylpyridin-2-yl)ethoxy]-6-methoxy-2-[3-trifluoromethyl-5-(2,2-dichloroacetamido)-benzyl]-3,4-dihydroisoquinolin-1(2H)-one (Compound 34)

[0161]

[0162] The product was prepared according to the method of Example 33 with a yield of 49.6%, except that difluorochloroacetic acid was replaced by dichloroacetic acid. 1 H NMR (600MHz, CDCl3) δ = 10.18 (s, 1H), 8.33 (d, J = 1.9, 1H), 7.93 (s, 1H), 7.59 (s, 1H), 7.36 (dd, J=7.9,2.3,1H),7.22(s,1H),7.19(d,J=7.9,1H),6.47(d,J=2.3,1H),6.23(s,1H),6.19(d,J =2.2,1H),4.71(s,2H),4.39(t,J=7.0,2H),3.82(s,3H),3.41(t,J=6.4,2H),3.23(t,J=7.0, 2H),2.78(t,J=6.4,2H),2.55(q,J=7.6,2H),1.18(dd,J=10.0,5.3,3H); HRMS(ESI):m / z[M+H] + :610.1497.

[0163] Example 36: 8-[2-(5-ethylpyridin-2-yl)ethoxy]-6-methoxy-2-[3-trifluoromethyl-5-(2,2,2-trichloroacetamido)-benzyl]-3,4-dihydroisoquinolin-1(2H)-one (Compound 35)

[0164]

[0165] The product was prepared according to the method of Example 33 with a yield of 22.4%, except that difluorochloroacetic acid was replaced by trichloroacetic acid. 1 H NMR(400MHz, CDCl3)δ=9.02-8.91(m,1H),8.37(s,1H),8.02(s,1H),7.76(s,1H),7 .50-7.43(m,1H),7.40(s,1H),7.37(s,1H),6.45(d,J=1.8,1H),6.26(s,1H),4.77 (s,2H),4.40(t,J=6.8,2H),3.80(s,3H),3.45(t,J=6.2,2H),3.35(t,J=6.8,2H), 2.84(t,J=6.1,2H),2.62(d,J=7.6,2H),1.22(t,J=7.6,3H); HRMS(ESI):m / z[M+H] + :644.1100.

[0166] Example 37: 8-[2-(5-ethylpyridin-2-yl)ethoxy]-6-methoxy-2-[3-trifluoromethyl-5-(2,2,2-trifluoroacetamido)-benzyl]-3,4-dihydroisoquinolin-1(2H)-one (Compound 36)

[0167]

[0168] The product was prepared according to the method of Example 33, except that difluorochloroacetic acid was replaced by trifluoroacetic acid. The yield was 36.9%. 1H NMR (400MHz, CDCl3) δ = 10.34 (s, 1H), 8.30 (d, J = 1.7, 1H), 7.95 (s, 1H), 7.75 (s, 1H), 7.32(d,J=6.3,1H),7.17(d,J=7.9,1H),6.42(d,J=2.2,1H),6.17(d,J=2.0,1H),4. 67(s,2H),4.35(t,J=6.8,2H),3.80(s,3H),3.43(t,J=6.3,2H),3.20(t,J=6.7,2H) ,2.80(t,J=6.3,2H),2.54(q,J=7.6,2H),1.17(t,J=7.6,3H); HRMS(ESI):m / z[M+H] + :596.1993.

[0169] Example 38: 8-[2-(5-ethylpyridin-2-yl)ethoxy]-6-methoxy-2-[3-trifluoromethyl-5-(2-chloroacetamido)-benzyl]-3,4-dihydroisoquinolin-1(2H)-one (Compound 37)

[0170]

[0171] Substituting difluorochloroacetic acid with chloroacetic acid, the product was prepared according to the method of Example 33 with a yield of 22.3%. 1 HNMR (600MHz, CDCl3) δ = 9.02 (s, 1H), 8.36 (d, J = 1.9, 1H), 7.88 (s, 1H), 7.71 (s, 1H), 7.44 (d d,J=7.9,1.9,1H),7.33(d,J=7.9,1H),7.30(s,1H),6.45(d,J=2.3,1H),6.25(d,J=2.2,1H) ,4.75(s,2H),4.40(t,J=6.9,2H),4.13(s,2H),3.80(s,3H),3.44(t,J=6.4,2H),3.33(t,J= 6.9,2H),2.84(t,J=6.3,2H),2.60(q,J=7.6,2H),1.21(t,J=7.6,3H); HRMS(ESI):m / z[M+H] + :576.1888.

[0172] Example 39: 8-[2-(5-ethylpyridin-2-yl)ethoxy]-6-methoxy-2-[3-trifluoromethyl-5-(2,2-difluoroacetamido)-benzyl]-3,4-dihydroisoquinolin-1(2H)-one (Compound 38)

[0173]

[0174] The product was prepared according to the method of Example 33 with a yield of 22.3%, except that difluorochloroacetic acid was used instead of difluoroacetic acid. 1 H NMR (600MHz, CDCl3) δ: 9.72 (s, 1H), 8.32 (d, J = 1.9, 1H), 7.91 (s, 1H), 7.71 (s, 1H), 7.35 (dd, J =7.9,2.1,1H),7.26(s,1H),7.21(d,J=7.9,1H),6.44(d,J=2.3,1H),6.19(d,J=2.2,1H),5.9 7(t,J=54.1,1H),4.70(s,2H),4.37(t,J=6.9,2H),3.80(s,3H),3.43(t,J=6.4,2H),3.23(t, J=6.9,2H),2.80(t,J=6.3,2H),2.55(q,J=7.6,2H),1.18(t,J=7.6,3H); HRMS(ESI):m / z[M+H] + :578.2083.

[0175] Example 40: 8-[2-(5-ethylpyridin-2-yl)ethoxy]-6-methoxy-2-[3-trifluoromethyl-5-(4-methyl-2-cyano-2-pentenylamido)-benzyl]-3,4-dihydroisoquinolin-1(2H)-one (Compound 39)

[0176]

[0177] The product was prepared according to the method of Example 33 with a yield of 25.4%, except that difluorochloroacetic acid was replaced by 4-methyl-2-cyano-2-pentenoic acid. 1H NMR (600MHz, DMSO-d6) δ: 9.29 (s, 1H), 8.19 (d, J = 1.4, 1H), 7.74 (t, J = 1.4, 1H), 7.49 (dd, J = 7.5, 1.6, 1H), 7.40 (t ,J=1.5,1H),7.32(t,J=1.4,1H),7.13(d,J=7.5,1H),7.04(d,J=1.4,1H),6.99(d,J=1.4,1H),6.95(d,J=6.0,1H ),4.61(s,2H),4.35(t,J=7.8,2H),3.87(t,J=5.5,2H),3.85(s,3H),3.81(t,J=5.6,1H),3.24(t,J=7.8,2H),2. 78(dd,J=12.6,6.2,1H),2.63(q,J=6.6,2H),1.33(t,J=6.6,3H),1.11(s,3H),1.10(s,3H); HRMS(ESI):m / z[M+H] + :621.2695.

[0178] The following experiments are used to illustrate the application of the representative compounds of the general formula (I) of the present invention in drugs for treating NAFLD.

[0179] Biological Example 1: In vitro human soluble epoxide hydrolase (hsEH) inhibitory activity assay

[0180] The hsEH-specific substrate (3-phenyl-oxiranyl)-acetic acid cyano-6-methoxy-naphthalen-2-yl)-methyl ester (PHOME) itself is non-fluorescent, but it can be hydrolyzed under the action of hsEH to generate the product 6-methoxy-2-naphthaldehyde, which can emit fluorescence at a wavelength of 465nm under 330nm light excitation. By measuring this fluorescence, the inhibitory activity of representative compounds against hsEH can be evaluated.

[0181] A fluorescence-based human soluble epoxide hydrolase assay kit (Cayman Chemical, Cat. No. 10011671) was used. According to the instructions, 185 μl of Bis-Tris buffer, 5 μl of test compound at different concentrations (TPPU as a positive control), 5 μl of recombinant hsEH, and 5 μl of 10 μM PHOME were added to a black 96-well plate and incubated at 25°C for 15 min. The product formation fluorescence (λem=330 nm, λex=465 nm) was measured by a Tecan Infinite F200 Pro plate reader. Each concentration was measured three times, and the IC was calculated using GraphPad Prism software. 50 .

[0182] Table 1. Results of human soluble epoxide hydrolase inhibitory activity assay

[0183]

[0184]

[0185] Biological Example 2: Therapeutic effects of representative compounds according to the present invention on diet-induced NAFLD in mice

[0186] 120 SPF male C57BL / 6J mice (7-8 weeks old, weight 20±2g) were randomly divided into a normal group (10 mice) and a model group (110 mice) after adaptive feeding for 1 week; NAFLD was induced by fructose, and the model group mice were given 30% fructose aqueous solution every day, and the normal group mice were given drinking water. After 4 weeks of induction, the model group was randomly divided into 11 groups (NALFD group, TPPU group, compound 4 group, compound 17 group, compound 21 group, compound 24 group, compound 27 group, compound 29 group, compound 36 group, compound 39 group, compound 40 group), among which the test compound and the positive control (TPPU) group were gavaged at 10 mg / kg body weight, and the normal group and the negative group were given the same volume of drinking water.

[0187] After 4 weeks of administration, the mice in the 14 groups were anesthetized, and the eyeballs were removed to collect peripheral blood. The plasma was collected after centrifugation, and the liver function and peripheral blood lipid content were tested by an automatic biochemical analyzer. After blood collection, the mice were killed by cervical dislocation, fixed on the operating table, and the skin and peritoneum were cut open with surgical scissors in turn to expose the liver and remove the liver tissue, fixed with paraformaldehyde, and stained with HE and Oil Red O to observe the pathological changes of liver tissue and the degree of fat accumulation. The photos taken are shown in the figure. Figure 1 and Figure 2 as shown in .

[0188] Table 2 Test results of triglyceride, total cholesterol, interleukin-6, alanine aminotransferase and aspartate aminotransferase in each group

[0189]

[0190] Based on the above test results and Figure 1 and Figure 2 The pathological changes in liver tissue and the degree of fat accumulation shown in the staining photos show that the representative compounds of the present invention all have inhibitory activity against hsEH, among which the IC values ​​of the enzyme inhibitory activity of compound 4, compounds 20-29, compounds 32, and compounds 34-39 are 50 <100nM, the enzyme inhibitory activity of compounds 26-29 and compound 39 is better than that of the positive control drug TPPU. Compared with the NALFD model group, the compounds of the present invention can improve the levels of triglycerides, total cholesterol, interleukin-6, alanine aminotransferase, and aspartate aminotransferase; compared with the positive control drug TPPU, compounds 29 and compound 39 show better reduction effects on triglycerides, total cholesterol, interleukin-6, alanine aminotransferase, and aspartate aminotransferase; liver tissue pathological sections show that the tested compounds 29 and 39 are superior to the NALFD model group and the positive control drug TPPU group in reversing liver tissue lesions and reducing fat accumulation. PPARγ and PTP1B inhibitors such as unsubstituted 3-phenylisoquinolinone derivatives with isopentenyl structures (compounds 40 and 41), styracone lactone derivatives (compound 42) or styracone acid A (compound 43) did not show hsEH inhibitory activity. Animal experiments showed that the 3-phenylisoquinolinone derivatives (compounds 40 and 41) failed to improve the relevant biochemical indexes of animals with non-alcoholic fatty liver disease. The above facts show that the N-substituted 3,4-dihydroisoquinolin-1(2H)-one derivatives according to the present invention act through specific drug targets and can be used to treat NALFD.

[0191] The above embodiments do not limit the present invention in any form. Any simple modification, equivalent change and modification made to the above embodiments according to the technical essence of the present invention without departing from the technical solution of the present invention still fall within the scope of the technical solution of the present invention.

Claims

1. A compound represented by formula (II) or a pharmaceutically acceptable salt thereof: R 1 Select from the following groups, where the wavy line Indicates the point of attachment of the group to the rest of the molecule: hydroxyl, methoxy, R 2 Select from the following groups, where the wavy line Indicates the point of attachment of the group to the rest of the molecule: H, R 3 and R 4 are each independently selected from H, halogen, hydroxy, nitro, amino, cyano, unsubstituted or optionally substituted with one, two or more R b Substituted with the following groups: C 1-6 Alkyl, C 1-6 Alkylcarbonylamino, C 1-6 Alkenylcarbonylamino, where R b Selected from halogen, cyano, C 1-6 Alkyl, nitro; R 5 Indicates H or C 1-6 alkyl.

2. The compound according to claim 1 or a pharmaceutically acceptable salt thereof, wherein R 3 and R 4 Each is independently selected from the following groups, wherein the wavy line Indicate the point of attachment of the group to the rest of the molecule: H, halogen, hydroxy, nitro, amino, cyano, unsubstituted or optionally substituted with one, two or more R b Substituted C 1-6 alkyl, 3. The following compound or a pharmaceutically acceptable salt thereof:

4. A pharmaceutical composition comprising a therapeutically effective amount of at least one of the compounds according to any one of claims 1 to 3 or pharmaceutically acceptable salts thereof.

5. The pharmaceutical composition according to claim 4, further comprising one or more pharmaceutically acceptable excipients.

6. The pharmaceutical composition according to claim 4 or 5, further comprising one or more additional therapeutic agents.

7. Use of the compound according to any one of claims 1 to 3 or a pharmaceutically acceptable salt thereof in the preparation of a medicament for treating diseases associated with soluble epoxide hydrolases.

8. The use according to claim 7, which is used in the preparation of a drug for treating, preventing or alleviating fatty liver.

9. The use according to claim 7, which is for preparing a drug for treating, preventing or alleviating NAFLD.

10. The use according to claim 7, which is for preparing a drug for treating, preventing or alleviating fatty hepatitis, liver fibrosis and liver cirrhosis.

11. Use of the compound according to any one of claims 1 to 3 or a pharmaceutically acceptable salt thereof in the preparation of a medicament for treating, preventing or alleviating NAFLD.

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