Preparation method of chicken manure composite fermentation microbial inoculum, fermentation microbial inoculum prepared thereby, chicken manure biological organic fertilizer, preparation method thereof and application

Abstract

The present invention discloses a preparation method of a chicken manure composite fermentation inoculant, a fermentation inoculant prepared thereby, a chicken manure bio-organic fertilizer, and a preparation method and application thereof, relating to the technical field of microbial applications. The preparation method of the inoculant includes: S1: fermenting six strains of bacteria, namely Bacillus.spHZ3, Geobacillus stearothermophilus, Pseudoxanthomonas taiwanensis, etc., respectively, to obtain fresh fermentation bacterial liquids of the six strains of bacteria; S2: mixing the fresh fermentation bacterial liquids of the six strains of bacteria according to a volume ratio of 3-5:1-3:1-2:2-3:2-5:1-3 to obtain a composite inoculant; S3: adding a flora promoter thereto to obtain the product. The preparation method of the bio-organic fertilizer includes (1) adding an oxidant and an activator to the chicken manure for pretreatment, (2) adding the inoculant thereto for fermentation, and (3) drying and pulverizing to obtain the product. Beneficial effects: The present invention couples the oxidation treatment technology with the microbial fermentation treatment, shortens the composting time, improves the conversion of organic matter, enhances the fertilizer efficiency of the organic fertilizer, and achieves the effect of rapidly preparing the organic fertilizer.

Description

Technical Field

[0001] The present invention relates to the technical field of microbial applications, and particularly relates to a preparation method of a chicken manure composite fermentation agent, a fermentation agent prepared thereby, a chicken manure biological organic fertilizer, and preparation methods and applications thereof. Background Art

[0002] With the continuous improvement of people's living standards, the demand for livestock and poultry products has also been increasing continuously. The growing market demand has also led to the gradual development of the livestock industry from traditional free-range farming to intensive and large-scale farming, and the breeding scale has been continuously expanding. Chicken manure contains rich nutrient substances, among which the crude protein content can reach 18.7%, the fat content can reach 2.5%, and the carbohydrate content can reach 11%. At the same time, chicken manure also contains cellulose (7%), 18 kinds of amino acids, a large number of trace elements, and a relatively high content of vitamin B12. More importantly, chicken manure also contains 2.34% nitrogen, 2.32% phosphorus, and 0.83% potassium, which is a relatively high ratio among manure fertilizers. Therefore, chicken manure is a high-quality organic fertilizer resource with very good application prospects.

[0003] In addition to generating unpleasant odors and causing discomfort, it is more likely to breed mosquitoes and accelerate the spread of avian diseases. Moreover, if unrotted chicken manure is used as organic fertilizer, high temperatures will be generated after application, causing root burning and seedling rot, breeding root-knot nematodes, and allowing other pathogens to invade the crop body and cause diseases. Therefore, chicken manure must be fully fermented by microorganisms and converted into organic fertilizer before it can be used. However, the traditional microbial composting method for fermenting chicken manure to produce organic fertilizer takes 15 - 20 days to fully rot the chicken manure, resulting in the occupation of a large amount of equipment and space in the farm or organic fertilizer production factory. In addition, when microorganisms decompose nitrogen-containing and sulfur-containing compounds in chicken manure, gases such as ammonia or hydrogen sulfide will inevitably be generated, which can irritate the mucous membranes of humans and animals. In severe cases, it can enter the blood through the mucous membranes or respiratory system, combine with hemoglobin, affect blood transportation, and thus cause complications.

[0004] Therefore, how to safely and quickly convert chicken manure into organic fertilizer, which can not only reduce the labor and material costs of farms or organic fertilizer manufacturers, but also avoid the adverse environmental impacts caused by the microbial fermentation of chicken manure, is of great significance for promoting the resource utilization of chicken manure and the production and application of chicken manure organic fertilizer. The present invention couples the oxidation treatment technology with the microbial fermentation treatment. By the oxidation treatment, the firm structure of the organic nutrients in chicken manure is quickly destroyed, and most of the organic matter is converted into small-molecule carbon sources, nitrogen sources and other substances, accelerating the humification of chicken manure. At the same time, the unconverted organic matter is further fermented and transformed by the microorganisms in the biological treatment section. While realizing the rapid reproduction of beneficial microorganisms (especially those with deodorizing functions), it further realizes the deep humification of chicken manure and reduces gases such as ammonia or hydrogen sulfide, producing high-quality biological organic fertilizer with high maturity and rich nutrient content.

[0005] The Chinese patent application document with the publication number CN111533586A discloses a chicken manure biological organic fertilizer and its preparation method. The composting raw materials include fresh chicken manure, dry distiller's grains, straw and a compound composting microbial agent; the compound composting microbial agent is cultured in a fermentation medium with dry distiller's grains enzymatic hydrolysate as the matrix; the dry distiller's grains enzymatic hydrolysate is obtained by enzymatically hydrolyzing a 5% - 15% dry distiller's grains solution; the amount of dry distiller's grains is 5% - 15% of the mass of fresh chicken manure. The preparation of the chicken manure biological organic fertilizer is as follows: fresh chicken manure, dry distiller's grains and straw are mixed, the C / N is adjusted to 20 - 24, and the moisture content is 50% - 60%. After mixing the compound composting microbial agent at 0.1% - 0.5% of the total mass of the compost pile, composting fermentation is carried out for 6 - 20 days to obtain it. This patent uses dry distiller's grains as nutrients. On the one hand, a large amount of released amino acids are used as nutrient components of the culture medium, and on the other hand, as composting auxiliary materials, it effectively reduces the difference in the nutrient environment, the production process is simple, and the quality of the biological organic fertilizer is higher. However, the production cycle is still relatively long, about 20 days, and the quality of its organic fertilizer still needs to be further improved. Summary of the Invention

[0006] The technical problem to be solved by the present invention is how to solve the problems of long composting time in the production of traditional chicken manure organic fertilizer, easy generation of odor gases such as hydrogen sulfide causing secondary pollution, and low fertilizer efficiency of traditional chicken manure organic fertilizer.

[0007] Strain description: The strains used in the microbial fermentation process of the present invention include:

[0008] Bacillus.spHZ3, which is preserved in the China Center for Type Culture Collection (CCTCC). The preservation address: Luojia Mountain, Bayi Road, Wuchang District, Wuhan City, Hubei Province. The preservation number: CCTCC NO: M 20221472, and the preservation date: September 21, 2022. This strain has excellent tolerance and passivation performance to heavy metals such as lead and copper.

[0009] Geobacillus stearothermophilus, preservation number: CGMCC 1.1865; Pseudoxanthomonas taiwanensis CGMCC 1.10867; Streptomyces corchorusii, preservation number: CGMCC 4.7392; Rhodococcus rhodochrous, preservation number: CGMCC 1.15298; Aspergillus oryzae, preservation number: CGMCC 3.13905 were all purchased from the China General Microbiological Culture Collection Center (CGMCC), and were screened by selective media such as cellulose-decomposing bacteria selection medium, milk agar medium, ammonia-removing medium, etc. Strains with good strain compatibility, strong ability to decompose organic matter, and good ammonia removal effect were selected for scientific combination. At the same time, the optimal addition ratio of each strain and the optimal addition amount of the bacterial flora promoter were studied.

[0010] The present invention realizes the solution to the above technical problems through the following technical means:

[0011] A preparation method of a chicken manure composite fermentation bacterium agent, comprising the following steps:

[0012] S1: Single-strain fermentation: Ferment six strains of Bacillus.spHZ3, Geobacillus stearothermophilus, Pseudoxanthomonas taiwanensis, Streptomyces corchorusii, Rhodococcus rhodochrous, and Aspergillus oryzae respectively to obtain fresh fermentation bacterial liquids of the six strains;

[0013] S2: Preparation of composite bacterium agent: Mix the fresh fermentation bacterial liquids of the six strains of Bacillus.spHZ3, Geobacillus stearothermophilus, Pseudoxanthomonas taiwanensis, Streptomyces corchorusii, Rhodococcus rhodochrous, and Aspergillus oryzae obtained in step S1 according to a volume ratio of (3-5):(1-3):(1-2):(2-3):(2-5):(1-3) to obtain a composite bacterium agent;

[0014] S3: Addition of bacterial flora promoter: Add a bacterial flora promoter to the composite bacterium agent obtained in step S2 to obtain the chicken manure composite fermentation bacterium agent.

[0015] Beneficial effects: The present invention prepares a composite fermentation bacterium agent that can efficiently ferment chicken manure by selecting six special strains, mixing them in a special ratio, and then adding a bacterial flora promoter, shortening the preparation time of chicken manure organic fertilizer and improving the fertilizer efficiency of chicken manure organic fertilizer.

[0016] Preferably, the specific operation steps for fermenting the six strains respectively in step S1 are:

[0017] Bacillus.spHZ3, Geobacillus stearothermophilus, and Pseudoxanthomonas taiwanensis were all cultured in LNG medium. The LNG medium consisted of 0.5% beef extract powder, 1% peptone, 0.5% yeast extract, 0.5% sodium chloride, and 1000 mL of pure water. They were inoculated into the LNG liquid medium at an inoculation amount of 1% (v / v), and cultured at 150 - 200 rpm for 12 - 24 h.

[0018] Streptomyces corchorusii and Rhodococcus rhodochrous were both cultured in SYP medium. The SYP medium consisted of 1.0% sucrose, 2.0% malt extract, 0.5% peptone, 1.0% soybean cake powder, 0.1% dipotassium hydrogen phosphate, 0.5% ammonium sulfate, 0.1% sodium chloride, 0.3% calcium carbonate, and 1000 mL of pure water. They were inoculated into the SYP liquid medium at an inoculation amount of 1% (v / v), and cultured at 150 - 200 rpm for 12 - 24 h.

[0019] Aspergillus oryzae was cultured in MSP medium. The MSP medium consisted of 3% corncob powder, 0.2% peptone, 0.4% KH2PO4, 0.04% (w / v) CaCl2, 0.04% MgSO4, and 1000 mL of pure water. It was inoculated into the MSP liquid medium at an inoculation amount of 2% (v / v), and cultured at 150 - 200 rpm for 12 - 24 h.

[0020] Preferably, in step S3, a flora promoter was added to the compound microbial agent obtained in step S2 at a ratio of 1 - 2% (w / v).

[0021] Preferably, the flora promoter in step S3 was prepared by mixing quercetin, tea polyphenols, and biotin in a mass ratio of (2 - 5) : (3 - 5) : (1 - 3).

[0022] The present invention also provides a chicken manure compound fermentation microbial agent prepared by the preparation method of the above chicken manure compound fermentation microbial agent.

[0023] (1) Oxidation treatment of chicken manure: An oxidant and an activator were added to fresh chicken manure and mixed thoroughly. After stirring at room temperature for a period of time, a preliminary conversion product of chicken manure was obtained.

[0024] Note: After the oxidation treatment of chicken manure, the obtained preliminary conversion product has a relatively high degree of organic matter stabilization and humification.

[0025] (2) Microbial fermentation of the preliminary chicken manure conversion product: Sprinkle the chicken manure compound fermentation inoculant prepared by the above method on the preliminary chicken manure conversion product obtained in step (1), mix well, adjust the water content of the preliminary chicken manure conversion product, ferment at room temperature, turn the pile once every once in a while. After several days of fermentation, when the preliminary chicken manure conversion product turns dark brown and the temperature drops to room temperature, stop fermentation to obtain the completely fermented chicken manure product;

[0026] (3) Preparation of chicken manure biological organic fertilizer: Dry and crush the completely fermented chicken manure product obtained in step (2) to obtain chicken manure biological organic fertilizer.

[0027] Beneficial effects: The present invention couples the oxidation treatment technology with the microbial fermentation treatment, significantly shortening the time required for the composting and decomposition of chicken manure, while improving the conversion of organic matter, enhancing the fertilizer efficiency of the organic fertilizer, and achieving the effect of quickly preparing high-quality biological fertilizer.

[0028] Preferably, the oxidant in step (1) is a mixture of three or more of sodium persulfate, potassium ferrate, calcium oxide, and magnesium oxide.

[0029] Preferably, the oxidant in step (1) is a mixture of sodium persulfate, calcium oxide, and magnesium oxide in a mass ratio of 3:1:2.

[0030] Preferably, the oxidant in step (1) is a mixture of sodium persulfate, potassium ferrate, calcium oxide, and magnesium oxide in a mass ratio of 3:2:1:1.

[0031] Preferably, the oxidant in step (1) is a mixture of potassium ferrate, calcium oxide, and magnesium oxide in a mass ratio of 3:1:1.

[0032] Preferably, the activator in step (1) is prepared by the following process: Dissolve FeSO4·7H2O in deoxygenated water, add diatomaceous earth, adjust the pH of the solution, shake, and then while dropping the KBH4 solution, continuously introduce N2 and stir, wash, and dry to obtain it; the mass ratio of FeSO4·7H2O to the volume of deoxygenated water is 3g / 100mL; the mass ratio of diatomaceous earth to FeSO4·7H2O is (6 - 10):15.

[0033] Preferably, the concentration of the KBH4 solution is 0.2mol / L.

[0034] Preferably, the activator in step (1) is prepared by the following process: Dissolve 1.5g of FeSO4·7H2O in 50mL of deoxygenated water, add 0.6g - 1.0g of diatomaceous earth, adjust the pH of the system to 4 - 6 with 0.5mol / L NaOH and HCl, and shake in a shaker for 10 - 18h to fully adsorb Fe 2+, Subsequently, 0.2 mol / L KBH4 solution was added dropwise (the dropping rate was 3 - 5 mL / min), while continuously introducing N2 and stirring for 6 - 8 h. The diatomaceous earth was quickly washed 2 - 3 times with absolute ethanol and deoxygenated water, and then dried to obtain the activator loaded with zero-valent iron.

[0035] Preferably, the stirring time in the step (1) is 60 - 120 min.

[0036] Preferably, the dosage of the chicken manure composite fermentation inoculant in the step (2) is: 5 - 10 mL of the chicken manure composite fermentation inoculant is sprayed into every 2000 g of the preliminary chicken manure conversion product.

[0037] Preferably, the water content of the preliminary chicken manure conversion product in the step (2) is adjusted to 50 - 60%.

[0038] Preferably, the compost is turned once every 8 - 16 h in the step (2).

[0039] The present invention also provides a chicken manure bio-organic fertilizer prepared by the above-mentioned rapid composting preparation method of chicken manure bio-organic fertilizer.

[0040] The present invention also provides an application of the chicken manure bio-organic fertilizer prepared by the above-mentioned rapid composting preparation method of chicken manure bio-organic fertilizer in agricultural production.

[0041] The advantages of the present invention are as follows:

[0042] (1) By selecting six special strains, mixing them in a special ratio, and then adding a bacterial community promoter, the present invention prepares a composite fermentation inoculant that can efficiently ferment chicken manure, shortening the preparation time of chicken manure organic fertilizer and improving the fertilizer efficiency of chicken manure organic fertilizer.

[0043] (2) By coupling the oxidation treatment technology with the microbial fermentation treatment, the present invention quickly destroys the firm structure of organic nutrients in chicken manure through oxidation treatment, generating small-molecule carbon sources, nitrogen sources and other substances. Subsequently, these nutrients are utilized by the microorganisms in the biological treatment section. The present invention not only realizes the rapid humification of chicken manure efficiently and quickly, but also can make full use of potential carbon sources, realizes the reproduction of beneficial microorganisms (especially microorganisms with biocontrol functions), and produces high-quality bio-organic fertilizers. Description of the Drawings

[0044] Figure 1 It is a comparative diagram of the influence of different composting treatment methods on the passivation effect of copper in chicken manure;

[0045] Figure 2 It is a comparative diagram of the three-dimensional fluorescence spectrum analysis before (A) and after (B) the oxidant treatment of chicken manure;

[0046] Figure 33D fluorescence spectrum analysis chart of the bio-organic fertilizer prepared in Example 5 of the present invention;

[0047] Figure 4 Comparison chart of the influence of the traditional compost of the bio-organic fertilizer prepared in Examples 4-6 of the present invention on the seed germination index;

[0048] Figure 5 Comparison chart of the influence of adding activator and not adding activator on the humification coefficient of chicken manure; Detailed implementation manners

[0049] To make the objectives, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the embodiments of the present invention. Obviously, the described embodiments are part of the embodiments of the present invention, rather than all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts belong to the scope of protection of the present invention.

[0050] The sodium persulfate, potassium ferrate, calcium oxide, and magnesium oxide used in the present invention are all purchased from the market and are all produced by Sinopharm Chemical Reagent Co., Ltd.; the diatomite is also purchased from the market and is produced by Shijiazhuang Huabang Mineral Products Co., Ltd.

[0051] Example 1:

[0052] A preparation method of a chicken manure composite fermentation bacterium agent, comprising the following steps:

[0053] S1: Single-strain fermentation: Ferment six strains of bacteria, namely Bacillus.spHZ3, Geobacillus stearothermophilus, Pseudoxanthomonas taiwanensis, Streptomyces corchorusii, Rhodococcus rhodochrous, and Aspergillus oryzae, respectively, to obtain fresh fermentation broth of the six strains of bacteria; among them, Bacillus.spHZ3, Geobacillus stearothermophilus, and Pseudoxanthomonas taiwanensis are all cultured in LNG medium, and the LNG medium is 0.5% beef extract powder, 1% peptone, 0.5% yeast extract, 0.5% sodium chloride, 1000 mL pure water; inoculate into the LNG liquid medium at an inoculation amount of 1% (v / v), 150 rpm, and culture for 24 h;

[0054] Streptomyces corchorusii and Rhodococcus rhodochrous are both cultured in SYP medium, and the SYP medium is 1.0% sucrose, 2.0% malt extract, 0.5% peptone, 1.0% soybean cake powder, 0.1% dipotassium hydrogen phosphate, 0.5% ammonium sulfate, 0.1% sodium chloride, 0.3% calcium carbonate, 1000 mL pure water; inoculate into the SYP liquid medium at an inoculation amount of 1% (v / v), 150 rpm, and culture for 24 h;

[0055] Aspergillus oryzae was cultured using MSP medium, which consisted of 3% corncob powder, 0.2% peptone, 0.4% KH2PO4, 0.04% (w / v) CaCl2, 0.04% MgSO4, and 1000 mL of pure water. It was inoculated into the MSP liquid medium at an inoculation amount of 2% (v / v), and cultured at 150 rpm for 24 h.

[0056] S2: Preparation of compound microbial agent: The fresh fermentation broth of six strains of bacteria, namely Bacillus.spHZ3, Geobacillus stearothermophilus, Pseudoxanthomonas taiwanensis, Streptomyces corchorusii, Rhodococcus rhodochrous, and Aspergillus oryzae obtained in step S1, were mixed according to a volume ratio of 3:1:1:2:2:1 to obtain a compound microbial agent.

[0057] S3: Addition of microbial community promoter: A microbial community promoter was added to the compound microbial agent obtained in step S2 at a ratio of 1% (w / v), and the chicken manure compound fermentation microbial agent was thus prepared. The microbial community promoter was prepared by mixing quercetin, tea polyphenols, and biotin according to a mass ratio of 2:3:1.

[0058] The chicken manure compound fermentation microbial agent of this example was named chicken manure compound fermentation microbial agent a.

[0059] Example 2:

[0060] A preparation method of a chicken manure compound fermentation microbial agent includes the following steps:

[0061] S1: Single-strain fermentation: Six strains of bacteria, namely Bacillus.spHZ3, Geobacillus stearothermophilus, Pseudoxanthomonas taiwanensis, Streptomyces corchorusii, Rhodococcus rhodochrous, and Aspergillus oryzae, were respectively fermented to obtain fresh fermentation broth of the six strains of bacteria. Among them, Bacillus.spHZ3, Geobacillus stearothermophilus, and Pseudoxanthomonas taiwanensis were all cultured using LNG medium, which consisted of 0.5% beef extract powder, 1% peptone, 0.5% yeast extract, 0.5% sodium chloride, and 1000 mL of pure water. They were inoculated into the LNG liquid medium at an inoculation amount of 1% (v / v), and cultured at 180 rpm for 18 h.

[0062] Both Streptomyces corchorusii and Rhodococcus rhodochrous were cultured using SYP medium, which consisted of 1.0% sucrose, 2.0% malt extract, 0.5% peptone, 1.0% soybean cake powder, 0.1% dipotassium hydrogen phosphate, 0.5% ammonium sulfate, 0.1% sodium chloride, 0.3% calcium carbonate, and 1000 mL of pure water. They were inoculated into the SYP liquid medium at an inoculation amount of 1% (v / v), and cultured at 180 rpm for 18 h.

[0063] Aspergillus oryzae is cultured using MSP medium, and the MSP medium consists of 3% corncob powder, 0.2% peptone, 0.4% KH2PO4, 0.04% (w / v) CaCl2, 0.04% MgSO4, and 1000 mL of pure water; it is inoculated into the MSP liquid medium at an inoculation amount of 2% (v / v), and cultured at 180 rpm for 18 h;

[0064] S2: Prepare the composite microbial agent: Mix the fresh fermentation broths of six strains of bacteria, namely Bacillus.spHZ3, Geobacillus stearothermophilus, Pseudoxanthomonas taiwanensis, Streptomyces corchorusii, Rhodococcus rhodochrous, and Aspergillus oryzae obtained in step S1 according to a volume ratio of 4:2:2:2:3:3 to obtain the composite microbial agent;

[0065] S3: Add the microbial community promoter: Add the microbial community promoter to the composite microbial agent obtained in step S2 according to a ratio of 1.5% (w / v), and the chicken manure composite fermentation microbial agent is thus prepared; the microbial community promoter is prepared by mixing quercetin, tea polyphenols, and biotin according to a mass ratio of 3:4:2.

[0066] Name the chicken manure composite fermentation microbial agent of this example as chicken manure composite fermentation microbial agent b.

[0067] Example 3:

[0068] A preparation method of a chicken manure composite fermentation microbial agent includes the following steps:

[0069] S1: Single-strain fermentation: Ferment six strains of bacteria, namely Bacillus.spHZ3, Geobacillus stearothermophilus, Pseudoxanthomonas taiwanensis, Streptomyces corchorusii, Rhodococcus rhodochrous, and Aspergillus oryzae, respectively, to obtain the fresh fermentation broths of the six strains of bacteria; among them, Bacillus.spHZ3, Geobacillus stearothermophilus, and Pseudoxanthomonas taiwanensis are all cultured using LNG medium, and the LNG medium consists of 0.5% beef extract powder, 1% peptone, 0.5% yeast extract, 0.5% sodium chloride, and 1000 mL of pure water; it is inoculated into the LNG liquid medium at an inoculation amount of 1% (v / v), and cultured at 200 rpm for 12 h;

[0070] Both Streptomyces corchorusii and Rhodococcus rhodochrous are cultured using SYP medium, and the SYP medium consists of 1.0% sucrose, 2.0% malt extract, 0.5% peptone, 1.0% soybean cake powder, 0.1% dipotassium hydrogen phosphate, 0.5% ammonium sulfate, 0.1% sodium chloride, 0.3% calcium carbonate, and 1000 mL of pure water; it is inoculated into the SYP liquid medium at an inoculation amount of 1% (v / v), and cultured at 200 rpm for 12 h;

[0071] Aspergillus oryzae is cultured using MSP medium. The MSP medium consists of 3% corncob powder, 0.2% peptone, 0.4% KH2PO4, 0.04% (w / v) CaCl2, 0.04% MgSO4, and 1000 mL of pure water. It is inoculated into the MSP liquid medium at an inoculation amount of 2% (v / v), and cultured at 200 rpm for 12 h.

[0072] S2: Preparation of compound microbial agent: The fresh fermentation broths of six strains of bacteria, namely Bacillus.spHZ3, Geobacillus stearothermophilus, Pseudoxanthomonas taiwanensis, Streptomyces corchorusii, Rhodococcus rhodochrous, and Aspergillus oryzae obtained in step S1 are mixed according to a volume ratio of 5:3:2:3:5:2 to obtain a compound microbial agent.

[0073] S3: Addition of microbial agent promoter: A microbial agent promoter is added to the compound microbial agent obtained in step S2 according to a ratio of 2% (w / v), and the chicken manure compound fermentation microbial agent is thus prepared. The microbial agent promoter is prepared by mixing quercetin, tea polyphenols, and biotin according to a mass ratio of 5:5:3.

[0074] The chicken manure compound fermentation microbial agent of this example is named chicken manure compound fermentation microbial agent c.

[0075] Example 4:

[0076] A rapid composting preparation method for chicken manure biological organic fertilizer includes the following steps:

[0077] (1) Oxidation treatment of chicken manure: 0.1 g of oxidant and 0.1 g of activator are added to fresh chicken manure, and they are fully mixed evenly. After stirring at room temperature for 120 min, a preliminary conversion product of chicken manure with a relatively high degree of organic matter stabilization and humification is obtained.

[0078] Among them, the oxidant is prepared by mixing sodium persulfate, calcium oxide, and magnesium oxide according to a mass ratio of 3:1:2; the activator is prepared through the following process: Dissolve 1.5 g of FeSO4·7H2O in 50 mL of deoxygenated water, add 0.6 g of diatomaceous earth, adjust the pH of the system to 4.5 with 0.5 mol / L NaOH and HCl, and oscillate in a shaker for 10 h to fully adsorb Fe 2+ , then, dropwise add 0.2 mol / L KBH4 solution (dropwise addition rate 3 mL / min), continuously introduce N2 and stir for 6 h at the same time, quickly wash the diatomaceous earth twice with absolute ethanol and deoxygenated water, and dry it to obtain the activator loaded with zero-valent iron.

[0079] (2) Microbial fermentation of the preliminary chicken manure conversion product: Spray 5 mL of the chicken manure composite fermentation inoculant a prepared in Example 1 onto 2000 g of the preliminary chicken manure conversion product obtained in step (1), mix well, adjust the water content of the preliminary chicken manure conversion product to 50%, ferment at room temperature, turn the pile once every 12 h. After 7 days of fermentation, the preliminary chicken manure conversion product turns dark brown and the temperature drops to room temperature, indicating that the fermentation is completed. Stop the fermentation to obtain the completely fermented chicken manure product;

[0080] (3) Preparation of chicken manure bio-organic fertilizer: Dry and crush the completely fermented chicken manure product obtained in step (2) to obtain chicken manure bio-organic fertilizer rich in beneficial bacteria.

[0081] The chicken manure bio-organic fertilizer prepared in this example was tested, and the test results were as follows:

[0082] The effective viable count of the prepared bio-organic fertilizer was determined according to the method in NY / T 2321—2013 "Inspection Rules for Microbial Fertilizer Products", and the effective viable count was 2.7×10 8 CFU·g -1 . The organic matter content, pH value and other indicators of the bio-organic fertilizer product were determined according to the method in NY884—2012 "Bio-organic Fertilizer". The organic matter content was 47.3%, and the pH was 7.1, meeting the technical index requirements of the bio-organic fertilizer product.

[0083] Example 5:

[0084] A rapid composting preparation method of chicken manure bio-organic fertilizer includes the following steps:

[0085] (1) Oxidation treatment of chicken manure: Add 0.3 g of oxidant and 0.15 g of activator to fresh chicken manure, mix well, stir at room temperature for 60 min to obtain a preliminary chicken manure conversion product with a relatively high degree of organic matter stabilization and humification;

[0086] Among them, the oxidant is prepared by mixing sodium persulfate, potassium ferrate, calcium oxide and magnesium oxide in a mass ratio of 3:2:1:1; the activator is prepared by the following process: Dissolve 1.5 g of FeSO4·7H2O in 50 mL of deoxygenated water, add 0.8 g of diatomaceous earth, adjust the pH of the system to 5 with 0.5 mol / L NaOH and HCl, and oscillate in a shaker for 14 h to fully adsorb Fe 2+ , then, dropwise add 0.2 mol / L KBH4 solution (dropwise addition rate 4 mL / min), continuously introduce N2 and stir for 7 h at the same time, quickly wash the diatomaceous earth 3 times with absolute ethanol and deoxygenated water, and dry to obtain the activator loaded with zero-valent iron;

[0087] (2) Microbial fermentation of the preliminary chicken manure conversion product: Spray 8 mL of the chicken manure composite fermentation inoculant b prepared in Example 2 onto 2000 g of the preliminary chicken manure conversion product obtained in step (1), mix well, adjust the water content of the preliminary chicken manure conversion product to 55%, ferment at room temperature, turn the pile once every 14 h. After 5 days of fermentation, the preliminary chicken manure conversion product turns dark brown and the temperature drops to room temperature, indicating the completion of fermentation. Stop fermentation to obtain the completely fermented chicken manure product;

[0088] (3) Preparation of chicken manure bio-organic fertilizer: Dry and pulverize the completely fermented chicken manure product obtained in step (2) to obtain chicken manure bio-organic fertilizer rich in beneficial bacteria.

[0089] The chicken manure bio-organic fertilizer prepared in this example was tested, and the experimental results are as follows:

[0090] The effective viable count of the prepared bio-organic fertilizer was determined according to the method in NY / T 2321—2013 "Inspection Procedures for Microbial Fertilizer Products", and the effective viable count was 6.2×10 8 CFU·g -1 . The organic matter content, pH value and other indicators of the bio-organic fertilizer product were determined according to the method in NY884—2012 "Bio-organic Fertilizer". The organic matter content was 54.3%, and the pH was 7.3, meeting the technical index requirements of the bio-organic fertilizer product.

[0091] Example 6:

[0092] A rapid composting preparation method of chicken manure bio-organic fertilizer includes the following steps:

[0093] (1) Oxidation treatment of chicken manure: Add 0.4 g of oxidant and 0.2 g of activator to fresh chicken manure, mix well, and stir at room temperature for 80 min to obtain a preliminary chicken manure conversion product with a higher degree of organic matter stabilization and humification;

[0094] Among them, the oxidant is prepared by mixing potassium ferrate, calcium oxide and magnesium oxide in a mass ratio of 3:1:1; the activator is prepared by the following process: Dissolve 1.5 g of FeSO4·7H2O in 50 mL of deoxygenated water, add 1.0 g of diatomite, adjust the pH of the system to 6 with 0.5 mol / L NaOH and HCl, and oscillate in a shaker for 18 h to fully adsorb Fe 2+ , then, dropwise add 0.2 mol / L KBH4 solution (dropwise addition rate 4 mL / min), continuously introduce N2 and stir for 7 h at the same time, quickly wash the diatomite 3 times with absolute ethanol and deoxygenated water, and dry to obtain the activator loaded with zero-valent iron;

[0095] (2) Microbial fermentation of the preliminary chicken manure conversion product: Sprinkle 10 mL of the chicken manure compound fermentation inoculant c prepared in Example 3 onto 2000 g of the preliminary chicken manure conversion product obtained in step (1), mix well, adjust the water content of the preliminary chicken manure conversion product to 60%, ferment at room temperature, turn the pile once every 16 h. After 6 days of fermentation, the preliminary chicken manure conversion product turns dark brown and the temperature drops to room temperature, indicating that the fermentation is completed. Stop the fermentation to obtain the completely fermented chicken manure product;

[0096] (3) Preparation of chicken manure biological organic fertilizer: Dry and crush the completely fermented chicken manure product obtained in step (2) to obtain chicken manure biological organic fertilizer rich in beneficial bacteria.

[0097] The chicken manure biological organic fertilizer prepared in this example was tested, and the test results were as follows:

[0098] The effective viable count of the prepared biological organic fertilizer was determined according to the method in NY / T 2321—2013 "Inspection Regulations for Microbial Fertilizer Products", and the effective viable count was 4.3×10 8 CFU·g -1 . The organic matter content, pH value and other indicators of the biological organic fertilizer product were determined according to the method in NY884—2012 "Biological Organic Fertilizer". The organic matter content was 49.8%, and the pH was 7.5, meeting the technical index requirements of the biological organic fertilizer product.

[0099] Example 7:

[0100] Implementation case of applying the biological organic fertilizer prepared in the present invention in wheat production

[0101] Test crop: Yangmai 158

[0102] Test fertilizers: The biological organic fertilizers prepared in Examples 4-6 and the organic fertilizers prepared by traditional composting methods, with the application rate of 3000 kg / hm 2 . The fertilizers were all applied as base fertilizers into the soil tillage layer at one time, and no topdressing was carried out during the whole maize growth period. Other management measures were the same as those in the local area. A blank control was set, that is, no fertilizer was applied.

[0103] Determination indexes: At the maize harvest stage, ears of 30 consecutive plants with consistent growth were collected from each plot to measure the yield components such as the number of ears and 1000-grain weight.

[0104] The experimental results are shown in Table 1 below:

[0105] Table 1 Effects of biological organic fertilizers prepared in different examples on wheat yield components and yield

[0106]

[0107] Figure 1It is a comparison chart of the passivation effect of different composting treatments on copper in chicken manure; it can be seen from the chart that the composting of oxidant treatment coupled with compound microbial agents can significantly increase the distribution rate of residual Cu.

[0108] Figure 2 It is a comparison chart of three-dimensional fluorescence spectroscopy analysis before (A) and after (B) the oxidant treatment of chicken manure; it can be seen from the chart that after the oxidant treatment, characteristic peaks with excitation wavelength / emission wavelength of 330–360 / 400–460 nm appear, indicating the formation of humic acid substances (as shown by the black arrow).

[0109] Figure 3 It is a three-dimensional fluorescence spectroscopy analysis chart of the bio-organic fertilizer prepared in Example 5 of the present invention; it can be seen from the chart that the bio-organic fertilizer prepared after oxidant treatment + microbial fermentation contains very rich humic acid (as shown by the black arrow).

[0110] Figure 4 It is a comparison chart of the influence of traditional composting of the bio-organic fertilizer prepared in Examples 4-6 of the present invention on the seed germination index; it can be seen from the chart that the bio-organic fertilizer prepared by the present invention significantly increases the seed germination index.

[0111] Figure 5 It is a comparison chart of the influence of adding an activator and not adding an activator on the humification coefficient of chicken manure. It can be seen from the chart that the humification coefficient of the bio-organic fertilizer prepared by adding an activator is significantly higher than that without adding an activator.

[0112] From the data in Table 1, we can see that in the bio-organic fertilizer test groups obtained in Examples 4-6 of the present invention, the wheat yield is much higher than that of the organic fertilizer of traditional composting and the blank test group. By changing the ratio and composition of the oxidant and the activator, or the proportion of the compound microbial agent, the fertilizer efficiency shows obvious changes. Therefore, the composition and preparation process of the compound microbial agent in the present invention are the key to realizing the stable fertilizer efficiency and soil conditioning effect of the present invention, and the effect will be weakened if either is lacking.

[0113] The above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit them; although the present invention has been described in detail with reference to the foregoing embodiments, those of ordinary skill in the art should understand that they can still modify the technical solutions recorded in the foregoing embodiments, or perform equivalent replacements on some of the technical features; and these modifications or replacements do not make the essence of the corresponding technical solutions deviate from the spirit and scope of the technical solutions of the various embodiments of the present invention.

Claims

1. A preparation method of a chicken manure composite fermentation bacterial agent, characterized in that, It includes the following steps: S1: Single-strain fermentation: Ferment six strains of bacteria, namely Bacillus.spHZ3, Geobacillus stearothermophilus, Pseudoxanthomonas taiwanensis, Streptomyces corchorusii, Rhodococcus rhodochrous, and Aspergillus oryzae, respectively, to obtain fresh fermentation broth of the six strains; the preservation numbers of the six strains of Bacillus.spHZ3, Geobacillus stearothermophilus, Pseudoxanthomonas taiwanensis, Streptomyces corchorusii, Rhodococcus rhodochrous, and Aspergillus oryzae are CCTCC NO: M 20221472, CGMCC 1.1865, CGMCC1.10867, CGMCC 4.7392, CGMCC 1.15298, and CGMCC 3.13905, respectively; S2: Preparation of compound microbial agent: Mix the fresh fermentation broth of the six strains of Bacillus.spHZ3, Geobacillus stearothermophilus, Pseudoxanthomonas taiwanensis, Streptomyces corchorusii, Rhodococcus rhodochrous, and Aspergillus oryzae obtained in step S1 according to a volume ratio of 3-5:1-3:1-2:2-3:2-5:1-3 to obtain a compound microbial agent; S3: Adding microbial flora promoter: Add a microbial flora promoter to the compound microbial agent obtained in step S2 to obtain a compound fermentation microbial agent for chicken manure; the microbial flora promoter is prepared by mixing quercetin, tea polyphenols, and biotin according to a mass ratio of 2-5:3-5:1-3.

2. The preparation method of the chicken manure composite fermentation inoculant according to claim 1, characterized in that, In step S3, the microbial flora promoter is added to the compound microbial agent obtained in step S2 according to a ratio of 1-2% w / v.

3. A compound fermentation microbial agent for chicken manure prepared by the preparation method according to any one of claims 1-2.

4. A rapid composting preparation method for chicken manure biological organic fertilizer, characterized in that, It includes the following steps: (1) Oxidation treatment of chicken manure: Add an oxidant and an activator to fresh chicken manure, mix well, stir at room temperature for a period of time, and obtain a preliminary conversion product of chicken manure; (2) Microbial fermentation of the preliminary conversion product of chicken manure: Spray the compound fermentation microbial agent for chicken manure according to claim 3 on the preliminary conversion product of chicken manure obtained in step (1), mix well, adjust the water content of the preliminary conversion product of chicken manure, ferment at normal temperature, turn the pile once every once in a while, after fermenting for several days, when the preliminary conversion product of chicken manure turns dark brown and the temperature drops to normal temperature, stop fermentation to obtain a completely fermented product of chicken manure; (3) Preparation of chicken manure bio-organic fertilizer: Dry and crush the completely fermented product of chicken manure obtained in step (2) to obtain chicken manure bio-organic fertilizer.

5. The rapid composting preparation method of the chicken manure biological organic fertilizer according to claim 4, characterized in that, The oxidant in step (1) is a mixture of three or more of sodium persulfate, potassium ferrate, calcium oxide, and magnesium oxide.

6. The rapid composting preparation method of the chicken manure biological organic fertilizer according to claim 4, characterized in that, The activator in step (1) is prepared by the following process: Dissolve FeSO4·7H2O in deoxygenated water, add diatomaceous earth, adjust the pH of the solution, shake, and then continuously introduce N2 and stir while dropping KBH4 solution, wash, and dry to obtain it; the mass ratio of FeSO4·7H2O to the volume of deoxygenated water is 3g / 100mL; the mass ratio of diatomaceous earth to FeSO4·7H2O is 6-10:

15.

7. The rapid composting preparation method of the chicken manure biological organic fertilizer according to claim 4, characterized in that, The stirring time in step (1) is 60-120 min.

8. The rapid composting preparation method of the chicken manure biological organic fertilizer according to claim 4, characterized in that, In the step (2), the dosage of the chicken manure composite fermentation inoculant is as follows: 5-10 mL of the chicken manure composite fermentation inoculant is sprayed into every 2000 g of the preliminary chicken manure conversion product; the water content of the preliminary chicken manure conversion product is adjusted to 50-60%; and the pile is turned over once every 8-16 h.

9. The chicken manure biological organic fertilizer prepared by the preparation method according to any one of claims 4-8.

10. The application of the chicken manure biological organic fertilizer prepared by the preparation method according to any one of claims 4-8 in agricultural production.

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