A sample analyzer and a control method thereof

Abstract

The application discloses a sample analyzer and a control method thereof. The sample analyzer comprises a flow chamber, a sampling module, a sheath liquid module, a driving module and a control module. The sampling module is connected to the flow chamber through a pipeline. The sheath liquid module comprises a sheath liquid pool configured to provide sheath liquid. The control method comprises the following steps: the control module controls the driving module to draw gas through the sampling module to form a first gas bubble; the control module controls the driving module to draw a sample through the sampling module; and the control module controls the driving module to transmit the first gas bubble and the sample to the flow chamber, and controls the sheath liquid module to transmit the sheath liquid in the sheath liquid pool to the flow chamber. Therefore, the sample and the sheath liquid in the pipeline can be separated through the first gas bubble, the particles in the sample can be prevented from diffusing into the sheath liquid, the loss of the particles can be reduced, the concentration of the sample entering the flow chamber can be maintained, and the accuracy of particle detection can be improved.

Description

Technical Field

[0001] This application relates to the field of sample detection technology, and in particular to a sample analyzer and its control method. Background Technology

[0002] With the development of academic research and clinical testing, the use of sample analyzers for sample testing has high practical value and can greatly help patients in the detection and treatment of infection types.

[0003] In existing technologies, flow cytometry is often used to detect the number of particles in a sample. However, mixing may occur after the sample and the sheath fluid come into direct contact. This usually causes particles in the sample to diffuse into the sheath fluid, resulting in lower detection accuracy. Summary of the Invention

[0004] The main objective of this application is to provide a sample analyzer and its control method, which aims to solve the technical problem of inaccurate sample detection in the prior art.

[0005] To address the aforementioned problems, this application provides a control method for a sample analyzer. The sample analyzer includes a flow chamber, a sampling module, a sheath fluid module, a drive module, and a control module. The sampling module is connected to the flow chamber via a pipeline. The sheath fluid module includes a sheath fluid pool for providing sheath fluid. The control method includes: the control module controlling the drive module to draw gas through the sampling module to form a first bubble; the control module controlling the drive module to draw a sample through the sampling module; the control module controlling the drive module to transfer the first bubble and the sample to the flow chamber, while simultaneously controlling the sheath fluid module to transfer the sheath fluid in the sheath fluid pool to the flow chamber. The first bubble is used to separate the sample and the sheath fluid.

[0006] To address the aforementioned problems, this application provides a sample analyzer, which includes a flow chamber, a sampling module, a sheath fluid module, a drive module, and a control module. The sampling module is connected to the flow chamber via a pipeline. The sheath fluid module includes a sheath fluid pool for providing sheath fluid. The control module is used to: control the drive module to draw gas through the sampling module to form a first bubble; control the drive module to draw a sample through the sampling module; control the drive module to transfer the first bubble and the sample to the flow chamber, and simultaneously control the sheath fluid module to transfer the sheath fluid in the sheath fluid pool to the flow chamber. The first bubble is used to separate the sample and the sheath fluid.

[0007] Compared with the prior art, this application provides a control method for a sample analyzer, wherein the sample analyzer includes a flow chamber, a sampling module, a sheath fluid module, a drive module, and a control module. The sampling module is connected to the flow chamber through a pipeline, and the sheath fluid module includes a sheath fluid pool for providing sheath fluid. The control method includes the control module controlling the drive module to draw gas through the sampling module to form a first bubble; the control module controlling the drive module to draw a sample through the sampling module; the control module controlling the drive module to transfer the first bubble and the sample to the flow chamber, and simultaneously controlling the sheath fluid module to transfer the sheath fluid in the sheath fluid pool to the flow chamber. Therefore, the sample and the sheath fluid in the pipeline can be separated by the first bubble, preventing particles in the sample from diffusing into the sheath fluid, thereby reducing particle loss and maintaining the concentration of the sample entering the flow chamber, thus improving the accuracy of particle detection. Attached Figure Description

[0008] To more clearly illustrate the technical solutions in the embodiments of this application or the prior art, the drawings used in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this application. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0009] Figure 1 This is a schematic diagram of the structure of an embodiment of the sample analyzer provided in this application;

[0010] Figure 2 This is a schematic diagram of the structure of an embodiment of the control method for the sample analyzer provided in this application;

[0011] Figure 3 This is a schematic diagram of the structure of an embodiment of the sample analyzer provided in this application;

[0012] Figure 4 This is a schematic diagram of the structure of an embodiment of the sample analyzer provided in this application;

[0013] Figure 5 This application provides Figure 4 The flowchart of an embodiment of the control method for the sample analyzer shown is illustrated.

[0014] Figure 6 This application provides Figure 4 The flowchart of an embodiment of the control method for the sample analyzer shown is illustrated.

[0015] Figure 7 This is a schematic diagram of the structure of an embodiment of the sample analyzer provided in this application;

[0016] Figure 8 This application provides Figure 7The flowchart of an embodiment of the control method for the sample analyzer shown is illustrated.

[0017] Figure 9 This is a schematic diagram of an embodiment of the sample analyzer provided in this application.

[0018] Reference numerals in the attached figures: Sample analyzer 10; Flow chamber 100; Sampling module 200; Sampling needle 210; Swab 220; Cleaning inlet 221; Cleaning outlet 222; Drive module 300; First syringe 310; First controllable valve 320; Second controllable valve 330; Third controllable valve 340; Second syringe 350; Sheath fluid module 400; Sheath fluid pool 410; Third syringe 420; Fourth controllable valve 430; Control module 500; First three-way valve 610; Second three-way valve 620; First solenoid valve 630; Fifth controllable valve 710; Second solenoid valve 720; Pump 730; Waste liquid collection device 740; Third solenoid valve 750; Waste liquid pool 760; Fourth solenoid valve 770; Third three-way valve 780. Detailed Implementation

[0019] The technical solutions of the embodiments of this application will be clearly and completely described below with reference to the accompanying drawings. It is understood that the specific embodiments described herein are only for explaining this application and not for limiting it. Furthermore, it should be noted that, for ease of description, only the parts related to this application are shown in the accompanying drawings, not all structures. All other embodiments obtained by those skilled in the art based on the embodiments of this application without creative effort are within the scope of protection of this application.

[0020] The terms "first," "second," and "third" in this application are for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of indicated technical features. Therefore, a feature defined as "first," "second," or "third" may explicitly or implicitly include at least one of that feature. In the description of this application, "multiple" means at least two, such as two, three, etc., unless otherwise explicitly specified. All directional indications (such as up, down, left, right, front, back, etc.) in the embodiments of this application are only used to explain the relative positional relationships and movements between components in a specific orientation (as shown in the figures). If the specific orientation changes, the directional indications also change accordingly. Furthermore, the terms "comprising" and "having," and any variations thereof, are intended to cover non-exclusive inclusion. For example, a process, method, system, product, or device that includes a series of steps or units is not limited to the listed steps or units, but may optionally include steps or units not listed, or may optionally include other steps or units inherent to these processes, methods, products, or devices.

[0021] In this document, the term "embodiment" means that a particular feature, structure, or characteristic described in connection with an embodiment may be included in at least one embodiment of this application. The appearance of this phrase in various places throughout the specification does not necessarily refer to the same embodiment, nor is it a separate or alternative embodiment mutually exclusive with other embodiments. It will be explicitly and implicitly understood by those skilled in the art that the embodiments described herein can be combined with other embodiments.

[0022] See Figure 1 , Figure 1 This is a schematic diagram of the structure of an embodiment of the sample analyzer 10 provided in this application.

[0023] This application provides a sample analyzer 10, which includes a flow chamber 100, a sampling module 200, a sheath fluid module 400, a drive module 300, and a control module 500. The sampling module 200 is connected to the flow chamber 100 via pipelines, and the sheath fluid module 400 includes a sheath fluid pool 410 for providing sheath fluid. The control module 500 is connected to the sampling module 200, the sheath fluid module 400, and the drive module 300.

[0024] The flow chamber 100 has a cavity, a sheath fluid inlet, a sample needle, and an outlet. The cavity is used for sample detection, and the sheath fluid inlet is used to connect to the sheath fluid module 400 via tubing, allowing sheath fluid to enter the cavity. One end of the sample needle is connected to the sampling module 200 via tubing. The sample is acquired by the sampling module 200 and enters the sample needle via tubing, ultimately entering a preset position within the cavity through the sample needle. The tubing connecting the sampling module 200 and the flow chamber 100 can store sheath fluid, which propels the sample. The sample can be blood or other fluids and can be stored in test tubes or other containers. The sampling module 200 collects the sample from the container.

[0025] In one embodiment, sheath fluid enters the cavity through the sheath fluid inlet, forming a sheath flow within the flow chamber 100. This sheath flow is laminar, providing better protection for the flow chamber 100. The sample flowing from the sample needle can pass stably and uniformly through the sheath flow, and the sheath flow provides better encapsulation and confinement of the flowing sample, allowing for the detection of particle counts during sample flow. However, in the prior art, when the sample flows through the sheath flow, the sheath fluid at the tip may mix with the sample. This typically causes particles in the sample to diffuse into the sheath fluid, resulting in lower detection accuracy.

[0026] See Figure 2 , Figure 2This is a schematic diagram of an embodiment of the control method for the sample analyzer 10 provided in this application. The control method of this embodiment is applied to the sample analyzer 10 described above, and includes the following steps:

[0027] Step S201: The control module 500 controls the drive module 300 to absorb gas through the sampling module 200 to form the first bubble.

[0028] When gas needs to be drawn through the sampling module 200, the sampling module 200 can be kept out of contact with the sample. The control module 500 controls the drive module 300 to operate, drawing gas through the sampling module 200 to form a first bubble in the pipeline between the sampling module 200 and the flow chamber 100. The gas can be air, oxygen, hydrogen, or other gases.

[0029] Step S202: The control module 500 controls the drive module 300 to absorb samples through the sampling module 200.

[0030] After the gas is drawn in by the sampling module 200 to form the first bubble, the control module 500 controls the sampling module to contact the sample and controls the drive module 300 to draw in the sample through the acquisition module, so that the sample comes into contact with the first bubble. The first bubble isolates the sample from the sheath fluid in the pipeline, so that the sample can be separated from the sheath fluid in the pipeline as it flows into the flow chamber 100, thus preventing particles in the sample near the first bubble from diffusing into the sheath fluid.

[0031] Step S203: The control module 500 controls the drive module 300 to transfer the first bubble and the sample to the flow chamber 100, and at the same time controls the sheath fluid module 400 to transfer the sheath fluid in the sheath fluid pool 410 to the flow chamber 100.

[0032] After the sampling module 200 collects the first bubble and the sample into the pipeline, the control module 500 can control the drive module 300 to transfer the first bubble and the sample to the flow chamber 100. At the same time, the sheath fluid module 400 controls the sheath fluid in the sheath fluid pool 410 to transfer the sheath fluid to the flow chamber 100, so that the sheath fluid forms a sheath flow in the cavity of the flow chamber 100, and the sample can pass through the sheath flow uniformly.

[0033] Therefore, in this embodiment, by separating the sample and the sheath fluid in the pipeline through the first bubble, even if the sample flows rapidly in the pipeline, particles in the sample can be prevented from diffusing into the sheath fluid, thereby reducing particle loss and maintaining the concentration of the sample entering the flow chamber, thus improving the accuracy of particle detection.

[0034] In one embodiment, after the step (step S202) in which the control module 500 controls the drive module 300 to absorb the sample through the sampling module 200, the control method further includes: the control module 500 controlling the drive module 300 to absorb the gas through the sampling module 200 to form a second bubble.

[0035] After the sample is collected by the sampling module 200, the control module 500 controls the collection module to separate from the sample and controls the drive module 300 to draw gas a second time through the collection module. The gas drawn a second time forms a second bubble, so that the sample is located between the first bubble and the second bubble, so as to isolate the sample from the sheath fluid through the first bubble and the second bubble.

[0036] The step (step S203) in which the control module 500 controls the drive module 300 to transfer the first bubble and the sample to the flow chamber 100 includes: the control module 500 controls the drive module 300 to transfer the first bubble, the sample and the second bubble to the flow chamber 100.

[0037] After the sampling module 200 collects the first bubble, the sample, and the second bubble into the pipeline, the control module 500 controls the drive module 300 to transfer the first bubble, the sample, and the second bubble to the flow chamber 100. At the same time, the sheath fluid module 400 controls the sheath fluid in the sheath fluid pool 410 to transfer the sheath fluid to the flow chamber 100, so that the sheath fluid forms a sheath flow in the cavity of the flow chamber 100, and the sample can pass through the sheath flow uniformly.

[0038] Therefore, in this embodiment, the first bubble prevents the front part of the sample from diffusing into the sheath fluid at the front of the sample, and the second bubble prevents the rear part of the sample from diffusing into the sheath fluid at the rear of the sample. This further avoids the sample at both ends diffusing into the sheath fluid, thereby reducing particle loss and maintaining the concentration of the sample entering the flow chamber 100, thus improving the accuracy of particle detection.

[0039] See Figure 3 , Figure 3 This is a schematic diagram of the structure of an embodiment of the sample analyzer 10 provided in this application.

[0040] The sample analyzer 10 includes a first three-way valve 610 and a second three-way valve 620. The first end of the first three-way valve 610 is connected to the drive module 300 through a pipeline, the second end of the first three-way valve 610 is connected to the flow chamber 100, the third end of the first three-way valve 610 is connected to the first end of the second three-way valve 620 through a pipeline, the second end of the second three-way valve 620 is connected to the sampling module 200 through a pipeline, and the third end of the second three-way valve 620 is connected to the drive module 300 through a pipeline.

[0041] Prior to the step where the control module 500 controls the drive module 300 to transfer the first bubble, the sample, and the second bubble to the flow chamber 100, the control method includes: the control module 500 controlling the drive module 300 to transfer the first bubble, the sample, and the second bubble to the pipeline located between the first three-way valve 610 and the second three-way valve 620.

[0042] The control module 500 controls the drive module 300 to apply suction from the end of the first three-way valve 610 connected to the drive module 300, conveying the first bubble, the sample, and the second bubble to the pipeline located between the first three-way valve 610 and the second three-way valve 620. Then, the control module 500 controls the drive module 300 to apply thrust from the end of the second three-way valve 620 connected to the drive module 300, conveying the first bubble, the sample, and the second bubble to the flow chamber 100.

[0043] Therefore, in this embodiment, by first transmitting the first bubble, the sample, and the second bubble to the pipeline located between the first three-way valve 610 and the second three-way valve 620, and then pushing them into the flow chamber 100, the first bubble, the sample, and the second bubble only need to flow within the pipeline connecting the sampling module 200 and the flow chamber 100 to be transmitted to the flow chamber 100. This reduces the length of the sample passing through the pipeline, reduces the amount of particle loss in the sample within the pipeline, and further improves the accuracy of sample detection.

[0044] See Figure 4 , Figure 4 This is a schematic diagram of the structure of an embodiment of the sample analyzer 10 provided in this application.

[0045] The drive module 300 includes a first controllable valve 320 and a first syringe 310. The first end of the first controllable valve 320 is connected to the first end of a first three-way valve 610 via a conduit. The second end of the first controllable valve 320 is connected to the third end of a second three-way valve 620 via a conduit. The common end of the first controllable valve 320 is connected to the first syringe 310 via a conduit. Further, the drive module 300 includes a second controllable valve 330. The first end of the second controllable valve 330 is connected to the common end of the first controllable valve 320 via a conduit. The second end of the second controllable valve 330 is connected to a sheath fluid reservoir 410 via a conduit. The common end of the second controllable valve 330 is connected to the first syringe 310 via a conduit.

[0046] Optionally, the sample analyzer 10 may further include a first solenoid valve 630, one end of which is connected to the second end of a second three-way valve 620 via a pipeline, and the second end of which is connected to the sampling module 200 via a pipeline. The control module 500 can control the first solenoid valve 630 to open or close the pipeline between the sampling module 200 and the first solenoid valve 630.

[0047] See Figure 5 , Figure 5 This application provides Figure 4 The control method of the sample analyzer 10 shown is illustrated in an embodiment flowchart.

[0048] The step of the control module 500 controlling the drive module 300 to transfer the first bubble, the sample, and the second bubble to the pipeline located between the first three-way valve 610 and the second three-way valve 620 includes the following steps:

[0049] Step S501: The control module 500 controls the first controllable valve 320 to connect the first syringe 310 and the first three-way valve 610, and controls the first syringe 310 to transfer the first bubble, the sample and the second bubble to the pipeline located between the first three-way valve 610 and the second three-way valve 620.

[0050] The control module 500 controls the first controllable valve 320 to connect the first syringe 310 and the first three-way valve 610, thereby connecting the tubing between the sampling module 200, the first three-way valve 610, the second three-way valve 620, the first controllable valve 320, and the first syringe 310. Furthermore, by controlling the first syringe 310 to provide suction, the first bubble, the sample, and the second bubble are transported to the tubing located between the first three-way valve 610 and the second three-way valve 620.

[0051] Furthermore, the step of the control module 500 controlling the drive module 300 to transfer the first bubble, the sample, and the second bubble to the flow chamber 100 includes the following steps:

[0052] Step S502: The control module 500 controls the first controllable valve 320 to connect the first syringe 310 and the second three-way valve 620, and controls the first syringe 310 to transfer the first bubble, the sample and the second bubble to the flow chamber 100.

[0053] The control module 500 controls the first controllable valve 320 to connect the first syringe 310 and the second three-way valve 620, so that the first syringe 310 can apply thrust along the path of the first syringe 310, the first controllable valve 320, the first three-way valve 610, and the second three-way valve 620, so as to transfer the first bubble, the sample and the second bubble to the flow chamber 100 by controlling the first syringe 310 to provide thrust.

[0054] Optionally, in one embodiment, when it is necessary to use the suction provided by the first syringe 310 to transfer the first bubble, sample, and second bubble to the tubing located between the first three-way valve 610 and the second three-way valve 620, the control module 500 can control the first solenoid valve 630 to open. When it is necessary to use the thrust provided by the first syringe 310 to transfer the first bubble, sample, and second bubble to the flow chamber 100, the control module 500 controls the first solenoid valve 630 to close.

[0055] See Figure 6 , Figure 6 This application provides Figure 4 The control method of the sample analyzer 10 shown is illustrated in an embodiment flowchart.

[0056] Before the step of control module 500 controlling the first controllable valve 320 to connect the first syringe 310 and the second three-way valve 620 (step S502), the control method includes the following steps:

[0057] Step S601: The control module 500 controls the second controllable valve 330 to connect the first syringe 310 and the sheath fluid pool 410, and controls the first syringe 310 to extract sheath fluid from the sheath fluid pool 410.

[0058] The control module 500 controls the second controllable valve 330 to connect the first syringe 310 and the sheath fluid pool 410, thereby connecting the pipeline between the first syringe 310, the second controllable valve 330, and the sheath fluid pool 410. Furthermore, the first syringe 310 is controlled to extract sheath fluid from the sheath fluid pool 410.

[0059] Step S602: The control module 500 controls the second controllable valve 330 to connect the first syringe 310 and the first controllable valve 320.

[0060] The control module 500 controls the second controllable valve 330 to connect the first syringe 310 and the first controllable valve 320, so that the first syringe 310, the second controllable valve 330, the first controllable valve 320 and the second three-way valve 620 are connected. Furthermore, the first syringe 310 is controlled to use sheath fluid to transfer the first bubble, the sample and the second bubble to the flow chamber 100.

[0061] See Figure 7 , Figure 7 This is a schematic diagram of the structure of an embodiment of the sample analyzer 10 provided in this application.

[0062] The drive module 300 also includes a third controllable valve 340 and a second syringe 350. The first end of the third controllable valve 340 is connected to the first syringe 310 through a pipeline, the second end of the third controllable valve 340 is connected to the sheath fluid pool 410 through a pipeline, and the common end of the third controllable valve 340 is connected to the second syringe 350 through a pipeline.

[0063] In this embodiment, the control module 500 can control the third controllable valve 340 to connect the second syringe 350 and the sheath fluid pool 410, so that the second syringe 350 draws sheath fluid from the sheath fluid pool 410. The control module 500 also controls the third controllable valve 340 to connect the second syringe 350 and the first syringe 310, so that the sheath fluid in the second syringe 350 is pushed into the first syringe 310 and the tubing connected to the first syringe 310.

[0064] See Figure 8 , Figure 8 This application provides Figure 7 The control method of the sample analyzer 10 shown is illustrated in an embodiment flowchart.

[0065] The steps of controlling the first syringe 310 to transfer the first bubble, the sample, and the second bubble to the tubing located between the first three-way valve 610 and the second three-way valve 620 include:

[0066] Step S801: The control module 500 controls the first syringe 310 and the second syringe 350 to transfer the first bubble, the sample and the second bubble to the pipeline located between the first three-way valve 610 and the second three-way valve 620.

[0067] The control module 500 controls the first controllable valve 320 to connect the first syringe 310 and the first three-way valve 610, thereby connecting the tubing between the sampling module 200, the first three-way valve 610, the second three-way valve 620, the first controllable valve 320, the first syringe 310, and the second syringe 350. Furthermore, by controlling the first syringe 310 and the second syringe 350 to provide suction, the first bubble, the sample, and the second bubble are transported to the tubing located between the first three-way valve 610 and the second three-way valve 620.

[0068] The steps of controlling the first syringe 310 to transfer the first bubble, the sample, and the second bubble to the flow chamber 100 include:

[0069] Step S802: The control module 500 controls the first syringe 310 and the second syringe 350 to transfer the first bubble, the sample and the second bubble to the flow chamber 100.

[0070] The control module 500 controls the first controllable valve 320 to connect the first syringe 310, the second syringe 350, and the second three-way valve 620, so that the first syringe 310 and the second syringe 350 can apply thrust along the path of the first syringe 310, the first controllable valve 320, the first three-way valve 610, and the second three-way valve 620, so as to transfer the first bubble, the sample, and the second bubble to the flow chamber 100 by controlling the first syringe 310 to provide thrust.

[0071] Therefore, in this embodiment, using both the first syringe 310 and the second syringe 350 to simultaneously transport the first bubble, the sample, and the second bubble to the flow chamber 100 can accelerate the sample flow rate and improve the efficiency of sample analysis. The first syringe 310 can be a small syringe, and the second syringe 350 can be a large syringe. The small syringe allows for precise positioning of the sample, while the large syringe provides greater suction or thrust, enabling rapid transport of the sample to the predetermined location and improving work efficiency.

[0072] Further, see Figure 4 and Figure 7 The sheath fluid module 400 also includes a third syringe 420 and a fourth controllable valve 430. The first end of the fourth controllable valve 430 is connected to the flow chamber 100 through a pipeline, the second end of the fourth controllable valve 430 is connected to the flow chamber 100 through a pipeline, and the common end of the fourth controllable valve 430 is connected to the third syringe 420 through a pipeline.

[0073] The step of controlling the sheath fluid module 400 to transfer the sheath fluid in the sheath fluid pool 410 to the flow chamber 100 includes: the control module 500 controlling the fourth controllable valve 430 to connect the third syringe 420 and the sheath fluid pool 410, and controlling the third syringe 420 to extract the sheath fluid from the sheath fluid pool 410. The control module 500 controlling the fourth controllable valve 430 to connect the third syringe 420 and the flow chamber 100, and controlling the third syringe 420 to transfer the sheath fluid in the third syringe 420 to the flow chamber 100.

[0074] Specifically, the control module 500 controls the fourth controllable valve 430 to connect the third syringe 420 and the sheath fluid pool 410, thereby connecting the pipeline between the third syringe 420, the fourth controllable valve 430, and the sheath fluid pool 410, so as to extract sheath fluid from the sheath fluid pool 410 by controlling the third syringe 420. After the third syringe 420 is filled with sheath fluid, the control module 500 controls the fourth controllable valve 430 to connect the third syringe 420 and the flow chamber 100, thereby connecting the pipeline between the third syringe 420, the fourth controllable valve 430, and the flow chamber 100, and controls the third syringe 420 to transfer the sheath fluid in the third syringe 420 to the flow chamber 100.

[0075] See Figure 9 , Figure 9 This is a schematic diagram of the structure of an embodiment of the sample analyzer 10 provided in this application.

[0076] The sample analyzer 10 also includes a cleaning module (not shown in the figure), and the control method also includes: the control module 500 controls the cleaning module to clean the sampling module 200.

[0077] The cleaning module includes a fifth controllable valve 710, a second solenoid valve 720, a pump 730, a waste liquid collection device 740, a third solenoid valve 750, a waste liquid pool 760, a fourth solenoid valve 770, and a third three-way valve 780. The sampling module 200 includes a sampling needle and a swab 220, with the swab 220 sleeved on the sampling needle. The swab 220 includes a cleaning inlet 221 and a cleaning outlet 222.

[0078] The first end of the fifth controllable valve 710 is connected to the first three-way valve 610 via a pipeline. The second end of the fifth controllable valve 710 is connected to the cleaning inlet 221 of the swab 220 via a pipeline. The common end of the fifth controllable valve 710 is connected to the first end of the first controllable valve 320 via a pipeline. The cleaning outlet 222 of the swab 220 is connected to one end of the second solenoid valve 720 via a pipeline. The other end of the second solenoid valve 720 is connected to the first end of the third three-way valve 780 via a pipeline. The second end of the third three-way valve 780 is connected to one end of the pump 730 via a pipeline. The pump 730 is connected to the waste liquid collection device 740 via a pipeline.

[0079] Therefore, when the outer wall of the sampling needle needs to be cleaned, the control module 500 can control the first syringe 310 to inject the sheath fluid of the first syringe 310 into the cleaning inlet 221 of the swab 220 through the tubing. After the outer wall of the sampling needle is cleaned by the swab 220 and the sheath fluid, the second solenoid valve 720 is turned on, and the pump 730 transfers the used sheath fluid from the cleaning outlet 222 of the swab 220 to the waste liquid collection device 740. When the inner wall of the sampling needle needs to be cleaned, the control module 500 controls the fifth controllable valve 710 to connect the first syringe 310, the first three-way valve 610, the second three-way valve 620, and the sampling needle. The first syringe 310 transfers the sheath fluid to the sampling needle. After the inner wall of the sampling needle is cleaned by the sheath fluid, the second solenoid valve 720 is turned on, and the pump 730 transfers the used sheath fluid from the cleaning outlet 222 of the swab 220 to the waste liquid collection device 740.

[0080] The third solenoid valve 750 is connected to the inlet of the flow chamber 100 and the waste liquid tank 760 through pipelines. The outlet of the waste liquid tank 760 is connected to the third end of the third three-way valve 780 through the fourth solenoid valve 770. After the flow chamber 100 has completed the detection, the control module 500 can control the third solenoid valve 750 to open, so as to transfer the liquid in the flow chamber 100 to the waste liquid tank 760. By controlling the fourth solenoid valve 770 to open, the liquid in the waste liquid tank 760 is transferred to the waste liquid collection device 740 through the pump 730.

[0081] Therefore, in this embodiment, after the sample analysis is completed through the flow chamber 100, the cleaning module can be controlled by the controller to clean the tubing and various components inside the sample analyzer 10, thereby facilitating the secondary use of the sample analyzer 10.

[0082] See Figure 1 This application also provides a sample analyzer 10, which includes a flow chamber 100, a sampling module 200, a sheath fluid module 400, a drive module 300, and a control module 500. The sampling module 200 is connected to the flow chamber 100 via a pipeline. The sheath fluid module 400 includes a sheath fluid pool 410 for providing sheath fluid. The control module 500 is connected to the sampling module 200, the sheath fluid module 400, and the drive module 300. The control module 500 controls the drive module 300 to draw gas through the sampling module 200 to form a first bubble. The control module 500 also controls the drive module 300 to draw a sample through the sampling module 200, and controls the drive module 300 to transfer the first bubble and the sample to the flow chamber 100. Simultaneously, the control module 500 controls the sheath fluid module 400 to transfer the sheath fluid in the sheath fluid pool 410 to the flow chamber 100.

[0083] Furthermore, the control module 500 is used to control the drive module 300 to draw gas through the sampling module 200 to form a second bubble. The control module 500 is also used to control the drive module 300 to transport the first bubble, the sample, and the second bubble to the flow chamber 100.

[0084] Therefore, in this embodiment, the first bubble prevents the sample at the front end from diffusing into the sheath fluid at the front end of the sample, and the second bubble prevents the sample at the rear end from diffusing into the sheath fluid at the rear end of the sample. This further avoids the sample at both ends diffusing into the sheath fluid, thereby reducing particle loss and maintaining the concentration of the sample entering the flow chamber 100, thus improving the accuracy of particle detection.

[0085] The sample analyzer 10 provided in this embodiment also includes the sample analyzer 10 provided in any of the above embodiments, which will not be described in detail here.

[0086] This document uses specific examples to illustrate the principles and implementation methods of this application. The descriptions of the above embodiments are only for the purpose of helping to understand the methods and core ideas of this application. At the same time, for those skilled in the art, there will be changes in the specific implementation methods and application scope based on the ideas of this application. Therefore, the content of this specification should not be construed as a limitation of this application.

Claims

1. A control method for a sample analyzer, characterized in that, The sample analyzer includes: a flow chamber, a sampling module, a sheath fluid module, a drive module, and a control module. The sampling module is connected to the flow chamber via a pipeline. The sheath fluid module includes a sheath fluid pool for supplying sheath fluid. The control method includes: The control module controls the drive module to draw in gas through the sampling module to form a first bubble; The control module controls the drive module to extract samples through the sampling module; The control module controls the drive module to transport the first bubble and the sample to the flow chamber, and simultaneously controls the sheath fluid module to transport the sheath fluid in the sheath fluid pool to the flow chamber; The control method further includes, after the step whereby the control module controls the drive module to extract the sample through the sampling module: The control module controls the drive module to draw in gas through the sampling module to form a second bubble; The step of the control module controlling the drive module to transfer the first bubble and the sample to the flow chamber includes: The control module controls the drive module to transport the first bubble, the sample, and the second bubble to the flow chamber.

2. The control method for the sample analyzer according to claim 1, characterized in that, The sample analyzer includes a first three-way valve and a second three-way valve. The first end of the first three-way valve is connected to the drive module through a pipeline, the second end of the first three-way valve is connected to the flow chamber, the third end of the first three-way valve is connected to the first end of the second three-way valve through a pipeline, the second end of the second three-way valve is connected to the sampling module through a pipeline, and the third end of the second three-way valve is connected to the drive module through a pipeline. Prior to the step where the control module controls the drive module to transfer the first bubble, the sample, and the second bubble to the flow chamber, the control method includes: The control module controls the drive module to transport the first bubble, the sample, and the second bubble to the pipeline located between the first three-way valve and the second three-way valve.

3. The control method for the sample analyzer according to claim 2, characterized in that, The drive module includes a first controllable valve and a first syringe. The first end of the first controllable valve is connected to the first end of the first three-way valve through a pipeline. The second end of the first controllable valve is connected to the third end of the second three-way valve through a pipeline. The common end of the first controllable valve is connected to the first syringe through a pipeline. The step of the control module controlling the drive module to transfer the first bubble, the sample, and the second bubble to the pipeline located between the first three-way valve and the second three-way valve includes: The control module controls the first controllable valve to connect the first syringe and the first three-way valve, so that the pipeline between the sampling module, the second three-way valve, the first three-way valve, the first controllable valve and the first syringe is connected, and controls the first syringe to transmit the first bubble, the sample and the second bubble to the pipeline located between the first three-way valve and the second three-way valve; The step of the control module controlling the drive module to transfer the first bubble, the sample, and the second bubble to the flow chamber includes: The control module controls the first controllable valve to connect the first syringe and the second three-way valve, so that the first syringe can apply thrust along the path of the first syringe, the first controllable valve, the second three-way valve and the first three-way valve, and controls the first syringe to transport the first bubble, the sample and the second bubble to the flow chamber.

4. The control method for the sample analyzer according to claim 3, characterized in that, The drive module includes a second controllable valve, the first end of which is connected to the common end of the first controllable valve via a pipeline, the second end of which is connected to the sheath fluid pool via a pipeline, and the common end of which is connected to the first syringe via a pipeline. Before the step of the control module controlling the first controllable valve to connect the first syringe and the second three-way valve, the control method includes: The control module controls the second controllable valve to connect the first syringe and the sheath fluid pool, and controls the first syringe to extract sheath fluid from the sheath fluid pool; The control module controls the second controllable valve to connect the first syringe and the first controllable valve.

5. The control method for the sample analyzer according to claim 3, characterized in that, The drive module includes a third controllable valve and a second syringe. The first end of the third controllable valve is connected to the first syringe through a pipeline, and the common end of the third controllable valve is connected to the second syringe through a pipeline. The step of controlling the first syringe to deliver the first bubble, the sample, and the second bubble to the tubing located between the first three-way valve and the second three-way valve includes: The control module controls the first and second syringes to transfer the first bubble, the sample, and the second bubble to the tubing located between the first three-way valve and the second three-way valve; The step of controlling the first syringe to deliver the first bubble, the sample, and the second bubble to the flow chamber includes: The control module controls the first syringe and the second syringe to transfer the first bubble, the sample and the second bubble to the flow chamber.

6. The control method for the sample analyzer according to any one of claims 1-5, characterized in that, The sheath fluid module also includes a third syringe and a fourth controllable valve. The first end of the fourth controllable valve is connected to the flow chamber via a pipeline, the second end of the fourth controllable valve is connected to the sheath fluid pool via a pipeline, and the common end of the fourth controllable valve is connected to the third syringe via a pipeline. The step of controlling the sheath fluid module to transfer the sheath fluid in the sheath fluid pool to the flow chamber includes: The control module controls the fourth controllable valve to connect the third syringe and the sheath fluid pool, and controls the third syringe to extract sheath fluid from the sheath fluid pool; The control module controls the fourth controllable valve to connect the third syringe and the flow chamber, and controls the third syringe to transfer the sheath fluid in the third syringe to the flow chamber.

7. The control method for the sample analyzer according to any one of claims 1-5, characterized in that, The sample analyzer also includes a cleaning module, and the control method further includes: The control module controls the cleaning module to clean the sampling module.

8. A sample analyzer, characterized in that, include: The system comprises a flow chamber, a sampling module, a sheath fluid module, a drive module, and a control module. The sampling module is connected to the flow chamber via piping. The sheath fluid module includes a sheath fluid pool for supplying sheath fluid. The control module is used for: The drive module is controlled to draw in gas through the sampling module to form a first bubble; The driving module is controlled to acquire samples through the sampling module; The drive module is controlled to transport the first bubble and the sample to the flow chamber, while the sheath fluid module is controlled to transport the sheath fluid in the sheath fluid pool to the flow chamber. The control module is further used for: The drive module is controlled to draw in gas through the sampling module to form a second bubble; The drive module is controlled to transport the first bubble, the sample, and the second bubble to the flow chamber.

Images