Adipose tissue regeneration substrate

Abstract

The object of the present invention is to provide an adipose tissue regeneration substrate that is highly operable and capable of regenerating a large volume of adipose tissue in a normal shape. The present invention is an adipose tissue regeneration substrate composed of a granular body and a bag-shaped body, wherein the granular body has an internal space and has a plurality of openings on the surface leading to the internal space and is composed of a bioabsorbable material, and the bag-shaped body has an opening and wraps a plurality of the granular bodies and is composed of a bioabsorbable material.

Description

Technical Field

[0001] The present invention relates to an adipose tissue regeneration substrate which has high operability and can regenerate a large volume of adipose tissue in a normal shape. Background Art

[0002] In the treatment of breast cancer, when the cancer formed in the breast cannot be cured by radiation or chemotherapy alone, a method using surgical resection (surgical therapy) is performed. At present, most of the total removal surgery is performed to remove all the breast tissue connected to the lesion, but in recent years, with the improvement of inspection technology, it is possible to detect the lesion as early as possible when it is small, so breast-conserving surgery that only removes part of the tissue can be performed. However, even with breast-conserving surgery, a depression will occur in the removed part, so it still puts a mental burden on the patient. Therefore, in order to improve QOL, more and more patients are undergoing breast reconstruction surgery after surgical therapy.

[0003] Silicone implants are mainly used in breast reconstruction surgery, but since they are non-bioabsorbable, they will remain in the body as foreign matter forever, and there is a possibility of postoperative leakage or infection due to rejection reactions, etc. In addition, there are concerns about adverse effects such as allergies and cancer caused by contact.

[0004] As another method, fat tissue can be collected from other parts and transplanted to the affected part with depression, but sometimes it is quickly absorbed after transplantation, causing depression again. In addition, since the collected tissue will cause new trauma, it is not necessarily preferred from the perspective of QOL.

[0005] In order to solve the problems of existing breast reconstruction surgery, the inventors disclosed a breast reconstruction component, in which a sponge containing collagen is enclosed inside a hollow granular body composed of polylactic acid (Patent Document 1). By inserting the breast reconstruction component of Patent Document 1 into the space created by partial mastectomy, surrounding cells can invade the breast reconstruction component and proliferate using the breast reconstruction component as a scaffold, and the breast can be reconstructed without transplanting fat tissue from other parts. In addition, since the breast reconstruction component of Patent Document 1 is made of bioabsorbable material, it will be slowly absorbed into the body as the breast regenerates and eventually disappears, so it is also highly safe.

[0006] Prior art literature

[0007] Patent Literature

[0008] Patent Document 1: Japanese Patent Application Publication No. 2016-140494 Summary of the invention

[0009] Technical problem to be solved by the invention

[0010] The breast reconstruction component of Patent Document 1 is very effective as a breast reconstruction surgery because it uses safe and living cells of the body to regenerate the breast. However, when the resection range of the breast reconstruction component of Patent Document 1 becomes larger, a large amount of breast reconstruction components need to be filled, so there is a problem of poor ease of implantation. In particular, as a step in the treatment of breast cancer, radiation therapy is performed at the same time after the cancer is removed in order to completely kill the cancer cells. Due to this effect, the skin will solidify, so the skin must be slowly expanded using a tissue expander, etc., and then cut open, and the implant must be inserted after the tissue expander is removed. In fact, in order to implant the small and large number of implants of Patent Document 1, and to implant them with a good appearance, a larger incision is required, which increases the burden on the patient.

[0011] In order to solve this technical problem, it is also considered to increase the size of the breast reconstruction component, but if the size of the breast reconstruction component becomes too large, the moldability will be reduced, and it will be difficult to regenerate fat tissue to the center, which will reduce the regeneration performance of the tissue. In addition, even if a large number of breast reconstruction components are inserted, each breast reconstruction component will move due to changes in posture or external forces, and the shape of the implanted part will easily collapse, so there is also a problem that it is difficult to regenerate a beautiful breast shape.

[0012] An object of the present invention is to provide an adipose tissue regeneration substrate that has high operability and can regenerate a large volume of adipose tissue in a normal shape.

[0013] Technical solutions to solve problems

[0014] The present invention is a fat tissue regeneration substrate composed of granules and bag-shaped bodies, wherein the granules have an internal space and have multiple openings on the surface leading to the internal space, and are composed of bioabsorbable materials; the bag-shaped body has an opening and wraps multiple granules, and is composed of bioabsorbable materials.

[0015] The present invention is described in detail below.

[0016] The inventor of the present invention conducted research and found that by stuffing a plurality of granular bodies made of bioabsorbable material into a bag-shaped body having an opening and made of bioabsorbable material, implantation is easy even when the resection site is large. In addition, even when force is applied from the outside, the shape is not easily collapsed and can be regenerated into normal-shaped tissue, thereby completing the present invention.

[0017] The adipose tissue regeneration substrate of the present invention is composed of granules and a bag body. The granules have an internal space and have multiple openings on the surface leading to the internal space, and are composed of bioabsorbable material. The bag body has an opening and wraps multiple granules, and is composed of bioabsorbable material.

[0018] Here, the schematic diagram of the adipose tissue regeneration substrate of the present invention and the above-mentioned granules is shown in Figure 1 , 2 .

[0019] like Figure 1 As shown, the adipose tissue regeneration substrate of the present invention is a structure in which a plurality of granules 1 are enclosed in a bag-shaped body 3. The granules 1 have an internal space and a plurality of openings leading to the internal space on the surface, and are in a closed shape as a whole. The bag-shaped body 3 has a plurality of openings and an internal space, and is in a closed shape in which the material enclosed inside does not move to the outside. Cells passing through the openings of the bag-shaped body 3 and the granules 1 proliferate using the wall surface inside the granules as a scaffold, thereby regenerating adipose tissue. In addition, since the granules 1 and the bag-shaped body 3 are composed of bioabsorbable materials, during the process of adipose tissue regeneration, the space of the adipose tissue to be regenerated is maintained, and after the adipose tissue is regenerated, it is absorbed by the body and eventually disappears. In the adipose tissue regeneration substrate of the present invention, by gathering a plurality of granules into the bag-shaped body, it is easier to implant in a larger space and has high operability. In addition, by wrapping a plurality of granules 1 in the bag-shaped body 3, the granules 1 will not be scattered in a wider range, so they can be implanted in a shape close to the shape after regeneration. In addition, even if a force is applied from the outside after implantation, the granular body 1 will not move to the outside of the bag-shaped body 3, so the shape at the time of implantation is not easily collapsed, and adipose tissue of normal shape can be regenerated. Moreover, in the adipose tissue regeneration substrate of the present invention, a sponge-like porous body 2 composed of a bioabsorbable material can be set inside the granular body 1. When a sponge-like porous body 2 composed of a bioabsorbable material is set inside the granular body 1, the regeneration of adipose tissue can be promoted due to the increase of cell scaffolds, and the strength can also be improved.

[0020] It should be noted that the sponge-like porous body is not limited to a sponge-like body, but also includes a shape having a plurality of voids such as non-woven fabric and cotton.

[0021] The bioabsorbable material constituting the above-mentioned granules is not particularly limited as long as the safety as an implant is confirmed, but since the regeneration of adipose tissue requires about half a year to one year, it is preferably a material that has the strength and decomposition rate that can maintain the space where the adipose tissue regeneration substrate is embedded during this period. As such a bioabsorbable material, collagen, gelatin, chitin, chitosan, etc. can be cited in natural polymers, and homopolymers of lactic acid, glycolic acid, ε-caprolactone, dioxanone, trimethylene carbonate, or copolymers composed of at least two or more materials selected from these can be cited in synthetic polymers. Among them, polylactic acid or a copolymer of lactic acid and other bioabsorbable materials is preferred because the strength and decomposition rate in the body are suitable for adipose tissue regeneration substrates. As the copolymers of the above-mentioned polylactic acid or lactic acid and other bioabsorbable materials, polylactide, copolymers of lactide and glycolic acid, and copolymers of lactide and ε-caprolactone described in Patent Document 1 can be cited.

[0022] When the bioabsorbable material of the granules is polylactide, a copolymer of lactide and glycolic acid, or a copolymer of lactide and ε-caprolactone, the weight average molecular weight is preferably 4000 to 300000. By making the weight average molecular weight within the above range, a material having a decomposition rate more suitable for the regeneration of adipose tissue can be obtained. The weight average molecular weight is more preferably 100000 or more, and more preferably 200000 or less.

[0023] The shape of the granules is not particularly limited as long as it can provide a scaffold for cell proliferation and maintain space for the adipose tissue to be regenerated, and may be spherical, columnar, or irregular shapes. Among them, a spherical shape is preferred, and an ellipsoidal spherical shape is more preferred, because the shape is not easily collapsed by external forces after implantation, and appropriate spaces are generated between the granules to further promote the regeneration of adipose tissue.

[0024] The size of the internal space is not particularly limited, but is preferably 10 mm 3 Above 100000mm 3 By setting the size of the internal space to the above range, it is possible to ensure space for regenerating adipose tissue and regenerate adipose tissue more reliably to the center of the granular body. The size of the internal space is preferably 25 mm. 3 More than 50 mm is more preferred 3 More than 50000mm is preferred 3 Below, more preferably 25000mm 3 the following.

[0025] The shape of the openings of the granular body is not particularly limited, and may be circular, lattice-shaped, polygonal, irregular, or the like.

[0026] In addition, the number of openings of the granules is not particularly limited as long as there are more than two. The size and occupancy of the openings of the granules are not particularly limited, as long as the cells can smoothly pass into the interior of the granules, and preferably the openings with a maximum length of 0.1 mm or more and 20 mm or less are distributed at an occupancy of 50% or more and 99% or less of the surface area of ​​the granules. When the size and occupancy of the openings are within the above range, the balance between the strength of the granules and the invasiveness of the cells can be further improved. The maximum length of the openings can be a size that fat tissue can invade and the surrounding tissues that already exist as tissues other than fat cannot invade, preferably 15 mm or less, more preferably 10 mm or less. In order to facilitate tissue invasion, the occupancy of the openings is preferably 60% or more of the surface area of ​​the granules, more preferably 70% or more, and from the viewpoint of ensuring the shape of the granules, it is preferably 95% or less, more preferably 90% or less. It should be noted that in this specification, the maximum length refers to the maximum length when the distance between two points of the opening is measured.

[0027] As a more specific form of the above-mentioned granular body, a mesh granular body, a porous capsule, etc. can be listed. In the case where the above-mentioned granular body is a mesh granular body, as the mesh constituting the above-mentioned granular body, a mesh (woven mesh), a woven fabric, a knitted fabric, etc. formed by a monofilament or a multifilament can be listed. Among them, from the viewpoints of elasticity, shape retention, and penetration into fat tissue, an ellipsoidal spherical body of the mesh is more preferred.

[0028] When the granular body is composed of a mesh, the thickness of each mesh constituting the granular body is not particularly limited, but from the viewpoints of the elasticity, shape retention, cell penetration, etc. of the mesh, it is preferably 0.05 mm to 1 mm, and more preferably 0.1 mm to 0.4 mm. The mesh size of the mesh constituting the granular body is preferably in the range of 0.01 mm to 6 mm in the longitudinal and transverse directions, respectively, and more preferably in the range of 0.02 mm to 5 mm.

[0029] The size of the granules is not particularly limited. When the granules are ellipsoidal, the major diameter is preferably 8 mm to 150 mm, and the minor diameter is 5 mm to 100 mm. By making the size of the granules within the above range, the shape can be easily adjusted when regenerating a large volume of adipose tissue, and the adipose tissue can be regenerated to the center more reliably. The major diameter of the granules is preferably 10 mm to 30 mm, and more preferably 15 mm to 20 mm. The minor diameter of the granules is preferably 5 mm to 20 mm, and more preferably 7 mm to 15 mm.

[0030] The number of the above-mentioned granules in the adipose tissue regeneration substrate of the present invention is not particularly limited as long as it is 2 or more, and can be appropriately adjusted according to the size of the granules and the size of the implantation space. From the viewpoint of operability and further promoting the regeneration of large volumes of adipose tissue, the number is preferably 5 or more, more preferably 10 or more, preferably 100 or less, and more preferably 50 or less.

[0031] The bioabsorbable material constituting the sponge-like porous body is not particularly limited, and examples thereof include synthetic polymers such as polyglycolide, polylactide, poly-ε-caprolactone, lactide-glycolic acid copolymer, glycolide-ε-caprolactone copolymer, lactide-ε-caprolactone copolymer, polycitric acid, polymalic acid, poly-α-cyanoacrylate, poly-β-hydroxy acid, polytrimethylene oxalate, polytetramethylene oxalate, polyorthoester, polyorthocarbonate, polyethylene carbonate, poly-γ-benzyl-L-glutamate, poly-γ-methyl-L-glutamate, poly-L-alanine, polyethylene glycol sebacate, polysaccharides such as starch, alginic acid, hyaluronic acid, chitin, pectic acid and its derivatives, and natural polymers such as proteins such as gelatin, collagen, albumin, and fibrin. Among them, collagen is preferably contained because of its high affinity with the organism.

[0032] When the sponge-like porous body contains collagen, it preferably contains 50% by weight or more of collagen. The content of collagen in the sponge-like porous body is more preferably 60% by weight or more, more preferably 70% by weight or more, more preferably 80% by weight or more, particularly preferably 90% by weight or more, very preferably 95% by weight or more, and most preferably 100% by weight.

[0033] The collagen can be any collagen derived from the skin, tendon, etc. of cattle, pigs, etc., without particular limitation. Among them, from the viewpoint of eliminating antigenicity and improving safety, it is preferred to treat collagen with an enzyme such as protease and pepsin to remove the telopeptide as much as possible to obtain atelocollagen.

[0034] Examples of commercially available products of the sponge-like porous body containing collagen include Pelnac (manufactured by Smith & Nephew Wound Management KK.) and Terudermis (manufactured by Terumo Corporation).

[0035] A granular body used as the adipose tissue regeneration substrate of the present invention is also one of the present invention. The granular body has an internal space and a plurality of openings on the surface leading to the internal space, and is made of a bioabsorbable material.

[0036] The shape of the bag-like body is not particularly limited, and a rectangular bag-like, a round bag-like, etc. can be used, and any shape can be used according to the ease of molding of the implantation position. In addition, as a specific mode, a net or a porous bag-like body formed by weaving a monofilament into a bag-like shape can be listed.

[0037] The bioabsorbable material constituting the above-mentioned bag-like body is not particularly limited. Since it is not necessary to maintain long-term strength than the granular body, the same material as the bioabsorbable material constituting the above-mentioned sponge-like porous body can be used. However, in order to maintain a plurality of granular bodies and maintain the overall shape, a stronger force is required, and as an implant, a material with a small amount of inflammatory reaction or rejection reaction is preferably used. As such a bioabsorbable material, a material that can be used as a suture can be listed, preferably polyglycolide, polylactic acid, polycaprolactone, polydioxane, trimethylene carbonate or these copolymers are used, and more preferably polyglycolide, a copolymer of polyglycolide and other bioabsorbable materials or a copolymer of lactic acid and other bioabsorbable materials are used.

[0038] When the bag-like body is a net, the thickness of the monofilament constituting the bag-like body is not particularly limited, but is preferably 0.01 mm or more, more preferably 0.1 mm or more, preferably 2 mm or less, more preferably 0.5 mm or less, from the viewpoint of the balance between flexibility and strength.

[0039] The occupancy rate of the opening of the bag-like body is not particularly limited as long as the cells can smoothly pass into the granules, but is preferably 50% to 99% of the surface area of ​​the bag-like body. When the occupancy rate of the opening is within the above range, the balance between the strength of the bag-like body and the invasiveness of the cells can be further improved. The occupancy rate of the opening of the bag-like body is preferably 60% to 60% of the surface area of ​​the bag-like body, more preferably 70% to 70%, more preferably 95% to 90%, and more preferably 90% to 90%.

[0040] The size of the opening of the bag-shaped body is not particularly limited as long as it does not hinder the invasion of cells into the granules and does not cause the granules to scatter outside the bag-shaped body. The maximum length of the opening of the bag-shaped body is preferably 1 / 50 times or more, more preferably 1 / 20 times or more, preferably 1 / 3 times or less, and more preferably 1 / 10 times or less of the short diameter of the granules. By making the size of the opening of the bag-shaped body within the above range, the shape imparting property and the operability of the obtained adipose tissue regeneration substrate as a whole can be further improved.

[0041] When the bag-like body is a net, specific numerical values ​​of the size of the mesh of the bag-like body are, for example, preferably 0.02 mm to 0.5 mm both in the longitudinal direction and the transverse direction, and more preferably 0.05 mm to 0.1 mm.

[0042] The size of the bag-shaped body can be appropriately adjusted according to the volume of the implanted part and the number of the granules. From the viewpoint of improving the moldability of the adipose tissue regeneration substrate and inhibiting the collapse of the granules after implantation, the internal space of the bag-shaped body is preferably 1.2 times or more, more preferably 1.5 times or more, preferably 3 times or less, and more preferably 2 times or less of the total volume of the granules. It should be noted that the total volume of the granules also includes the volume of the internal space of the granules.

[0043] The manufacturing method of the adipose tissue regeneration substrate of the present invention is not particularly limited, and can be manufactured in the following manner, that is, the sponge-like porous body is wrapped with a mesh body composed of a bioabsorbable material and the end is closed, thereby manufacturing a plurality of granules, and the obtained granules are wrapped with a bag-like body composed of a bioabsorbable material and the end is closed. In addition, it is also possible to insert the sponge-like porous body material from the opening after manufacturing the granules. The method of closing the end of the mesh body or the bag-like body is not particularly limited, and for example, a method of tying monofilaments to each other, hot pressing, etc. can be cited.

[0044] The adipose tissue regeneration substrate of the present invention is used for implanting adipose tissue to regenerate adipose tissue. By using the present invention, living adipose tissue composed of one's own cells can be regenerated without implanting tissue in other parts. Examples of adipose tissue that can be used with the present invention include breasts, buttocks, abdomen, etc. Among them, since the present invention can regenerate a large volume of adipose tissue in a normal shape, it can play a significant effect in the use of implanting it into a defective part caused by partial mastectomy to regenerate the breast.

[0045] Effects of the Invention

[0046] According to the present invention, it is possible to provide an adipose tissue regeneration substrate that has high operability and can regenerate a large volume of adipose tissue in a normal shape. BRIEF DESCRIPTION OF THE DRAWINGS

[0047] Figure 1 Schematic diagram of the adipose tissue regeneration substrate of the present invention.

[0048] Figure 2 is a schematic diagram of a granular body.

[0049] Figure 3 This is a diagram showing the measurement results of the moldability with respect to hole-shaped defects.

[0050] Figure 4 This is a diagram showing the measurement results of the moldability with respect to horizontal defects.

[0051] Figure 5These are magnetic resonance images (MRI) taken 0 (immediately after transplantation), 1, 3, 6, and 9 months after the adipose tissue regeneration matrix obtained in Example 1 was transplanted into the defect on the fascia of a pig.

[0052] Figure 6 This is a hematoxylin and eosin (HE)-stained image of the transplanted part 6 months after the adipose tissue regeneration matrix obtained in Example 1 was transplanted into the defect part on the fascia of a pig.

[0053] Figure 7 This is an Oil Red O-stained image of the transplanted part 6 months after the adipose tissue regeneration matrix obtained in Example 1 was transplanted into the defect part on the fascia of a pig.

[0054] Figure 8 This is an Azan-stained image of the transplanted part 6 months after the adipose tissue regeneration matrix obtained in Example 1 was transplanted into the defect part on the fascia of a pig.

[0055] Fig. 9 This is an anti-CD31 antibody immunostaining image of the transplanted part 6 months after the adipose tissue regeneration matrix obtained in Example 1 was transplanted into the defect part on the fascia of a pig. DETAILED DESCRIPTION

[0056] The embodiments of the present invention will be described in more detail below with reference to examples, but the present invention is not limited to these examples.

[0057] (Example 1)

[0058] Collagen sponge (Pelnac, manufactured by Smith & Nephew Wound Management KK.) was wrapped with a mesh (monofilament thickness: 0.2 mm to 0.25 mm, mesh opening: 1×1 mm to 2×2 mm) composed of polylactic acid (weight average molecular weight: 220,000), and the ends were sealed by heat-compression bonding to obtain elliptical spherical granules with a long diameter of 18 mm and a short diameter of 7.5 mm having collagen sponge inside. Thereafter, 30 granules were prepared by the same method, and the obtained granules were wrapped with an envelope-shaped bag-shaped body (monofilament composition: 0.015 mm×12, mesh size: 0.05 mm×0.05 mm) composed of 110 mm×35 mm polyglycolide multifilaments, and the ends were sealed by heat-fusion bonding to obtain a fat tissue regeneration substrate.

[0059] (Example 2)

[0060] An adipose tissue regeneration substrate was obtained in the same manner as in Example 1 except that the collagen sponge was not used.

[0061] (Comparative Example 1)

[0062] Thirty granules of Example 1 were used directly as a substrate for adipose tissue regeneration.

[0063] <Evaluation>

[0064] The following evaluations were performed on the adipose tissue regeneration substrates obtained in Examples and Comparative Examples.

[0065] (Evaluation of tissue regeneration 1)

[0066] The back of a mini pig (about 20 kg) was subcutaneously incised, and the adipose tissue regeneration matrix obtained in Examples 1 and 2 was implanted on the left side of the midline. Four months later, the portion where the adipose tissue regeneration matrix was implanted was removed to confirm the presence or absence of tissue regeneration, and the result showed that about 4 cm of tissue had been regenerated.

[0067] (Evaluation of tissue regeneration 2)

[0068] As experimental animals, miniature pigs (about 25 kg) of large animals were prepared, and the skin of the abdomen was cut in the middle. After that, the fat and mammary tissue on the left and right sides of the abdomen were peeled off, and a defect was made under the mammary gland and on the fascia. The adipose tissue regeneration substrate obtained in Example 1 was transplanted into the defect on the fascia, and the skin was sutured.

[0069] After surgery, magnetic resonance imaging (MRI) images of the abdomen were taken at 0 (immediately after transplantation), 1, 3, 6, and 9 months. The taken magnetic resonance imaging (MRI) images are shown in Figure 5 .

[0070] In addition, 6 months after the operation, the fat tissue above the rib layer on the right side of the abdomen was removed and the transplanted part was removed. The obtained specimen was prepared into a section specimen and subjected to hematoxylin and eosin (HE) staining, oil red O staining, Azan staining and anti-CD31 antibody immunostaining. The microscopic images of each staining are shown in Figure 6 , Figure 7 , Figure 8 and Fig. 9 .

[0071] from Figure 5 It can be seen that 6 months after the operation, in the area where the adipose tissue regeneration substrate was transplanted, regeneration of adipose tissue from the peripheral area connected to the adipose tissue and mammary tissue was confirmed (in Figure 5 MRI images (T1 enhanced images) show white parts). In addition, 9 months after the operation, regeneration of fat tissue from the periphery of the fat tissue regeneration matrix was confirmed in a larger area.

[0072] And, from Figure 6 , Figure 7 and Figure 8It can be seen that 6 months after the operation, the formation of adipose tissue and collagen tissue was confirmed inside the adipose tissue regeneration matrix. Fig. 9 It was found that blood vessels were formed in the adipose tissue and collagen tissue.

[0073] (Evaluation of Formability)

[0074] (1) Formability of hole defects

[0075] As a substitute for skin and adipose tissue, 358 g of chicken breast with skin was prepared, and the skin was partially peeled off to expose the meat. Afterwards, a cross was cut on the exposed meat, and a hole-shaped defect was made by digging out the central part. The skin was restored to its original position, and the length (longitudinal and transverse) of the incision and the height of the defect were measured. Afterwards, the adipose tissue regeneration substrate obtained in Example 1 was implanted in the defect, and after the skin was restored to its original position, the length (longitudinal and transverse) of the incision and the height of the defect were measured.

[0076] Then, 30 adipose tissue regeneration substrates of Comparative Example 1 were used to measure the length of the incision (vertical and horizontal) and the height of the defect using the same method. The measurement results are shown in Figure 3 From the measurement results, it can be seen that the adipose tissue regeneration substrate of Comparative Example 1 enters the incision site and is difficult to be formed in the height direction, while the adipose tissue regeneration substrate of Example 1 does not spread in the vertical and horizontal directions, but is densely packed in the form of a mountain in the height direction, so it is easy to form a shape with height, and has excellent formability for adipose tissues such as breasts and buttocks.

[0077] (2) Formability of horizontal defects

[0078] As a substitute for skin and fat tissue, 379g of chicken breast with skin was prepared, and the skin was peeled off to expose the meat. Then, an incision was made in the direction of the muscle fibers of the exposed meat, and the length (transverse), length (vertical) when the incision was opened, and the height of the incision were measured. Then, the adipose tissue regeneration substrate obtained in Example 1 was implanted in the incision, and the length (vertical, transverse) and height of the incision were measured. Then, 30 adipose tissue regeneration substrates of Comparative Example 1 were used, and the length (vertical, transverse) and height of the incision were measured using the same method.

[0079] At this time, the implanted state of the adipose tissue regeneration substrate in Example 1 and Comparative Example 1 was observed, and the result showed that no adipose tissue regeneration substrate leaked out from the incision in Example 1, but a plurality of adipose tissue regeneration substrates protruded and fell off from the incision in Comparative Example 1. It should be noted that the measurement of Comparative Example 1 was performed after the protruding and fallen adipose tissue regeneration substrate was pressed into the incision.

[0080] The measurement results are shown in Figure 4 From the measurement results, it can be seen that the fat tissue regeneration substrate of Comparative Example 1 will expand along the longitudinal direction of the incision and is difficult to be formed in the height direction, but Example 1 is not easy to expand in the longitudinal and transverse directions, and can be densely packed in the form of a mountain in the height direction, so it is easy to form a shape with height, and has excellent formability for fat tissues such as breasts and buttocks.

[0081] Industrial Applicability

[0082] According to the present invention, it is possible to provide an adipose tissue regeneration substrate that has high operability and is capable of regenerating a large volume of adipose tissue in a normal shape.

[0083] Explanation of symbols

[0084] 1: granular body; 2: sponge-like porous body; 3: bag-like body.

Claims

1. A substrate for regenerating fat tissue, Features: It is composed of granular bodies and bag-like bodies. The granular body has an internal space and a plurality of openings on the surface leading to the internal space, and is made of a bioabsorbable material. The bag-shaped body has a plurality of openings and encloses a plurality of the granules, and is made of a bioabsorbable material. The granular body has a sponge-like porous body made of a bioabsorbable material inside a mesh body that is in a closed shape as a whole. The bag-shaped body is a bag-shaped net.

2. The adipose tissue regeneration substrate according to claim 1, Features: The granules are ellipsoidal spheres containing polylactic acid or a copolymer of lactic acid and other bioabsorbable materials.

3. The adipose tissue regeneration substrate according to claim 1 or 2, Features: The bioabsorbable material constituting the bag-shaped body is polyglycolide, a copolymer of polyglycolide and other bioabsorbable materials, or a copolymer of lactic acid and other bioabsorbable materials.

4. The adipose tissue regeneration substrate according to claim 1 or 2, Features: It is used to implant in the defect caused by partial breast removal.

Images