Amino acid yield-related protein encoding gene pyrG and related strains, biological materials and applications thereof

Abstract

The application discloses amino acid yield related protein pyrG and a biomaterial and application thereof, and belongs to the technical field of biotechnology. The problem to be solved by the application is to regulate the amino acid yield of microorganisms, and the amino acid can be L-threonine, L-tryptophan, L-arginine and L-valine. The protein is any one of the following proteins: A1) a protein with an amino acid sequence in which aspartic acid at the 450th position of sequence 2 is replaced by a sequence of histidine, serine, glycine, alanine, valine or phenylalanine; A2) a protein with more than 80% identity with the protein shown in A1) and having the function of regulating the amino acid yield of microorganisms, which is obtained by substitution, deletion and / or addition of amino acid residues on the amino acid sequence of A1); and A3) a fusion protein with the same function, which is obtained by connecting a label to the N terminal and / or C terminal of A1) or A2). The substance for regulating the expression of the pyrG protein gene can be used for regulating the amino acid yield of microorganisms or breeding of an amino acid yield strain.