Liquid dispensing apparatus and method and use

Abstract

The application discloses a liquid dispensing device and a liquid dispensing method and application. The device comprises sequentially connected liquid injection components, a main body and liquid discharge components. The main body comprises a pressing plate, a liquid distribution cavity and a playback liquid component penetrating through the liquid distribution cavity arranged from top to bottom. The liquid injection components are used for conveying the liquid to be dispensed to the liquid distribution cavity. The liquid distribution cavity and the playback liquid component are closable or separable. When the liquid distribution cavity and the playback liquid component are closed, the liquid to be dispensed is quantitatively dispensed and the quantitatively dispensed liquid is stored. The pressing plate is used for separating the liquid distribution cavity and the playback liquid component by applying external force. The stored quantitatively dispensed liquid flows along the outer wall of the playback liquid component and flows to the liquid transfer plate. The liquid discharge components are used for discharging the residual liquid in the liquid distribution cavity. The liquid dispensing device and method can continuously and efficiently divide and dispense the liquid, and the device has low cost.

Description

TECHNICAL FIELD

[0001] The present application relates to a liquid dispensing device and method and application. BACKGROUND

[0002] The liquid dispensing device is mainly used for quantitative transfer and equal division of liquid to a pipette tray. It mainly includes quantitative suction, directional transfer and discharge of solution. In the existing pipetting device, mainly single-channel and multi-channel pipetting devices are manually or automatically operated.

[0003] The commonly used manual single-channel and multi-channel pipetting devices are mainly pipetting guns and discharge guns, etc. For example, 8-channel discharge guns are used to complete the liquid dispensing operation of 96-well plates, which needs to be operated 12 times to complete, having the disadvantages of time-consuming and labor-consuming.

[0004] A commonly used automatic multi-channel pipetting device, such as CN112960632B discloses a fully automatic sub-packaging system, which comprises a workbench (1), a robot hand (2) and a sub-packaging turntable (3) are installed on the upper part of the workbench (1), a cryopreservation box is arranged on the upper part of the sub-packaging turntable (3), a plurality of groups of insertion holes for placing cryopreservation tubes are uniformly arranged on the upper part of the cryopreservation box, and an injection sub-packaging device is arranged at the end of the robot hand (2); the injection sub-packaging device comprises an injection pump (4) fixedly connected to the end of the robot hand, a syringe (41) is clamped on the injection pump (4), and a torque sensing device (5) for detecting the pulling force of the injection pump (4) on the syringe (41) is further arranged on the injection pump (4); the torque sensing device (5) comprises a mounting seat (51) arranged in the length direction of the syringe (41), the mounting seat (51) is fixedly connected with a clamping block for clamping the syringe (41) on the injection pump (4), a sliding rod (52) is arranged in the length direction of the syringe (41) in the mounting seat (51), the sliding rod (52) is slidingly connected with the mounting seat (51), a push piece (53) is fixedly connected to the sliding rod (52), the push piece (53) is located at the middle position of the sliding rod (52), two guide rods (54) are fixedly connected to the mounting seat (51), the two guide rods (54) are arranged in parallel with the sliding rod (52) and located on both sides of the sliding rod (52), the two guide rods (54) penetrate through the push piece (53) and are slidingly connected with the push piece (53), two buffer springs (55) are sleeved on the sliding rod (52), the two buffer springs (55) are located on both sides of the push piece (53), two control contacts (56) are arranged on the side of the mounting seat (51) close to the push piece (53), the two control contacts (56) are located on one side of the sliding rod (52), and the two control contacts (56) are located on both sides of the push piece (53). In the multi-channel pipetting device, the robot hand combined with the injection pump is the most common structure, but this structure requires high automation degree and high device cost. Even if the structure of the robot hand combined with the injection pump is not used, other automatic multi-channel pipetting devices also have the problem of high price.

[0005] In summary, the existing liquid sub-packaging device mainly has the problems of time-consuming and labor-consuming operation, high device price. SUMMARY

[0006] In view of the problems existing in the prior art, the present application provides a liquid sub-packaging device and method, which can continuously sub-packaging liquid, is efficient and labor-saving, and has low device cost.

[0007] The first aspect of the present application provides a liquid dispensing device, which comprises a liquid injection component 1, a main body 2 and a liquid discharge component 3 connected in sequence, wherein the main body 2 comprises a pressing plate 21, a liquid distribution chamber 22 and a liquid return component 23 penetrating through the liquid distribution chamber 22 arranged from top to bottom, wherein the liquid injection component 1 is used to deliver the liquid to be dispensed to the liquid distribution chamber 22; the liquid distribution chamber 22 and the liquid return component 23 are closable or separable, when the liquid distribution chamber 22 and the liquid return component 23 are closed, the liquid to be dispensed is quantitatively dispensed and the quantitatively dispensed liquid is stored; the pressing plate 21 is used to separate the liquid distribution chamber 22 and the liquid return component 23 by applying an external force, and the quantitatively dispensed liquid stored in the liquid distribution chamber 22 flows along the outer wall of the liquid return component 23 downward and flows to a liquid transfer plate 4; the liquid discharge component 3 is used to discharge the remaining liquid in the liquid distribution chamber 22.

[0008] According to the liquid dispensing device of the present application, the remaining liquid in the liquid distribution chamber 22 can be discharged by the liquid discharge component 3, for example after the liquid dispensing is completed.

[0009] According to the liquid dispensing device of the present application, the liquid dispensing device can be used for continuous production operations such as batch detection of medical reagents, for example, dispensing liquid, especially dispensing liquid to a well plate, such as but not limited to a 24-well plate, a 96-well plate or a 384-well plate.

[0010] According to the liquid dispensing device of the present application, preferably, the bottom plate of the liquid distribution chamber 22 is arranged in a layout of high in the middle and low at both ends in the direction perpendicular to the liquid injection component 1 and the liquid discharge component 3.

[0011] According to the liquid dispensing device of the present application, preferably, the bottom plate of the liquid distribution chamber 22 is provided with a flow guide groove, the flow guide groove is in communication with the liquid injection component 1 and the liquid discharge component 3 at both ends respectively, and the height of the liquid injection component 1 is higher than the height of the liquid discharge component 3. Preferably, the flow guide groove is arranged in the middle of the bottom plate of the liquid distribution chamber 22.

[0012] According to the liquid dispensing device of the present application, preferably, the flow guide groove is arranged in a slope α in the horizontal direction. The α angle can be determined according to the physical properties of the liquid, such as viscosity, etc., preferably the α angle is 1-60°, preferably 5-45°, more preferably 5-30°.

[0013] According to the liquid dispensing device of the present application, preferably, the liquid dispensing cavity 22 is provided with a plurality of holes, each of which comprises a boss 221, a flow groove 222 and a liquid storage component 223; wherein the boss 221 protrudes from the hole opening of the hole, for preventing the liquid to be dispensed from flowing into the hole; the flow groove 222 is arranged on the boss 221, for making the liquid to be dispensed flow into the hole for quantitative dispensing to form a quantitatively dispensed liquid. In the present application, the number of holes is determined according to the needs, for example, when the liquid dispensing target is a 96-well plate, 96 holes corresponding to the 96-well plate can be provided.

[0014] According to the liquid dispensing device of the present application, preferably, the playback liquid component 23 comprises a plurality of tube bodies 231 matched with the plurality of holes of the liquid dispensing cavity 22, each tube body 231 is provided with a liquid locking component 232 on the side wall, and each tube body 231 is provided with a tapered component 233 at the lower end; the top of each tube body 231 is in contact with the pressing plate 21; the liquid locking component 232 is used for sealing connection with the boss 221 and preventing the liquid to be dispensed from flowing into the hole.

[0015] According to the liquid dispensing device of the present application, when the liquid dispensing cavity 22 is sealed with the playback liquid component 23, the liquid to be dispensed flows into the hole through the flow groove 222, completes quantitative dispensing of the liquid and is stored in the space formed by the liquid storage component 223 and the playback liquid component 23. In the present application, the volume of the space formed by the liquid storage component 223 and the playback liquid component 23 is the same as the target volume of each well of the liquid dispensing target, for example, the target volume of each well of a 96-well plate is 250 μL, and the volume of the space formed by each liquid storage component 223 and the playback liquid component 23 is 250 μL.

[0016] According to the liquid dispensing device of the present application, preferably, the top of each tube body 231 is in contact with the pressing plate 21.

[0017] According to the liquid dispensing device of the present application, preferably, the liquid locking component 232 is used for sealing connection with the boss 221 and preventing the liquid to be dispensed from flowing into the hole.

[0018] According to the liquid dispensing device of the present application, preferably, when the liquid dispensing cavity 22 is in a sealed state with the playback liquid component 23, the quantitatively dispensed liquid is stored in the space formed by the liquid storage component 223 and the playback liquid component 23. For example, an external force presses the pressing plate 21, and the tube body 231 in contact with the pressing plate 21 moves downward, at this time, the liquid dispensing cavity 22 is separated from the playback liquid component 23, and the liquid stored in the space formed by the liquid storage component 223 and the playback liquid component 23 flows downward along the outer wall of the tapered component 233 and flows to the pipetting plate 4, completing the quantitative dispensing of the liquid. In the present application, the pipetting plate 4 is the liquid dispensing target, for example, but not limited to, the pipetting plate 4 is a 24-well plate, a 96-well plate or a 384-well plate, etc.

[0019] According to the liquid dispensing device, preferably, the diameter of the liquid locking component 232 is greater than the diameter of the convex 221. When the external force presses the pressing plate 21, the tube 231 in contact with the pressing plate 21 is pressed downward, and at this time, the liquid locking component 232 is sealed and connected with the convex 221, thereby preventing the liquid to be dispensed from flowing into the hole.

[0020] According to the liquid dispensing device, preferably, the liquid locking component 232 is provided with an exhaust hole.

[0021] According to the liquid dispensing device, preferably, the liquid locking component 232 is provided with a sealing ring inside.

[0022] According to the liquid dispensing device, preferably, the outer wall of the tapered component 233 is provided with a sealing ring 234. The sealing ring provided on the outer wall of the tapered component 233 is used to ensure the airtightness of the space formed by the liquid storage component 223 and the playback liquid component 23.

[0023] According to the liquid dispensing device, preferably, a partition is provided between the liquid injection component 1 and the liquid separation cavity 22 of the main body 2. In the present application, the liquid injection component 1 can be a liquid injection groove, a liquid injection pipe, a liquid injection port, etc.

[0024] According to the liquid dispensing device, preferably, a sealing plug 31 is provided between the liquid discharge component 3 and the liquid separation cavity 22 of the main body 2. In the present application, the liquid discharge component 3 can be a liquid discharge groove, a liquid discharge pipe, a liquid discharge port, etc., which can achieve the purpose of discharging the remaining liquid in the liquid separation cavity 22.

[0025] According to the liquid dispensing device, preferably, the liquid dispensing device further comprises a spring 24 for supporting the pressing plate 21. The position of the spring can be determined according to the need, so as to achieve the purpose of supporting the pressing plate 21.

[0026] According to the liquid dispensing device, according to the need, the pressing plate 21 can be provided with a plurality of holes matched with a plurality of holes of the liquid separation cavity 22.

[0027] According to one embodiment of the present application, the liquid dispensing device comprises, but is not limited to, a liquid injection component 1, a main body 2 and a liquid discharge component 3 connected in sequence, wherein the main body 2 comprises, from top to bottom, a pressing plate 21, a liquid distribution chamber 22 and a playback liquid component 23 penetrating through the liquid distribution chamber 22, wherein the liquid injection component 1 is used to deliver the liquid to be dispensed to the liquid distribution chamber 22; the liquid distribution chamber 22 is sealable or separable from the playback liquid component 23, and when the liquid distribution chamber 22 is sealed with the playback liquid component 23, the liquid to be dispensed is quantitatively dispensed and stored; the pressing plate 21 is used to separate the liquid distribution chamber 22 from the playback liquid component 23 by applying an external force, and the stored quantitatively dispensed liquid flows down along the outer wall of the playback liquid component 23 and flows to a pipetting plate 4; and the liquid discharge component 3 is used to discharge the remaining liquid in the liquid distribution chamber 22.

[0028] In the embodiment, the bottom plate of the liquid distribution chamber 22 is arranged in a layout of high in the middle and low at both ends in the direction perpendicular to the liquid injection component 1 and the liquid discharge component 3; the bottom plate of the liquid distribution chamber 22 is provided with a flow guide groove, and the flow guide groove is in communication with the liquid injection component 1 and the liquid discharge component 3 at both ends, respectively, and is arranged in a slope α in the horizontal direction, and the height of the liquid injection component 1 is higher than the height of the liquid discharge component 3.

[0029] In the embodiment, the liquid distribution chamber 22 is provided with a plurality of holes, and each hole comprises a boss 221, a flow slot 222 and a liquid storage component 223; wherein the boss 221 protrudes from the hole of the hole, and is used to prevent the liquid to be dispensed from flowing into the hole; the flow slot 222 is arranged on the boss 221, and is used to make the liquid to be dispensed flow into the hole for quantitative dispensing to form quantitatively dispensed liquid.

[0030] In the embodiment, the playback liquid component 23 comprises a plurality of pipe bodies 231 matched with the plurality of holes of the liquid distribution chamber 22, a liquid locking component 232 arranged on the side wall of each pipe body 231, and a tapered component 233 arranged at the lower end of each pipe body 231; the top of each pipe body 231 is in contact with the pressing plate 21; the liquid locking component 232 is used to be in sealing connection with the boss 221 and prevent the liquid to be dispensed from flowing into the hole; the liquid locking component 232 is provided with an exhaust hole, and is provided with a sealing ring inside; and the outer wall of the tapered component 233 is provided with a sealing ring 234.

[0031] In the embodiment, a partition plate is arranged between the liquid injection component 1 and the liquid distribution chamber 22 of the main body 2; a sealing plug 31 is arranged between the liquid discharge component 3 and the liquid distribution chamber 22 of the main body 2; and the liquid dispensing device further comprises a spring 24 used to support the pressing plate 21.

[0032] When the pressing plate 21 is pressed by an external force, the spring is compressed, the tube body 231 in contact with the pressing plate 21 is downward, the playback liquid component 23 is downward as a whole, at this time, the liquid storage cavity 22 is separated from the playback liquid component 23, the liquid stored in the space formed by the liquid storage component 223 and the playback liquid component 23 flows downward along the outer wall of the conical component 233 and flows to the liquid transfer plate 4, at the same time, the liquid locking component 232 of the playback liquid component 23 is in sealing connection with the boss 221 to prevent the liquid to be distributed from flowing into the hole, and the quantitative and uniform distribution of the liquid is ensured. When the external force is removed, the spring 24 is bounced up, the tube body 231 in contact with the pressing plate 21 is upward, the playback liquid component 23 penetrating the liquid storage cavity 22 is upward as a whole, so that the liquid storage cavity 22 is sealed with the playback liquid component 23, the liquid to be distributed enters the hole through the flow groove 222, the quantitative distribution of the liquid is completed and stored in the space formed by the liquid storage component 223 and the playback liquid component 23. When the liquid transfer plate 4 is replaced and the pressing plate 21 is pressed by an external force again, the spring is compressed, the tube body 231 in contact with the pressing plate 21 is downward, and then the liquid storage cavity 22 is separated from the playback liquid component 23, the liquid stored in the space formed by the liquid storage component 223 and the playback liquid component 23 flows downward along the outer wall of the conical component 233 and flows to the liquid transfer plate 4, at the same time, the liquid locking component 232 of the playback liquid component 23 is in sealing connection with the boss 221 to prevent the liquid to be distributed from flowing into the hole, and the quantitative and uniform distribution of the liquid is ensured. According to the needs, the cycle is repeated for several times, so that the liquid distribution device of the application can continuously distribute the liquid.

[0033] The second aspect of the application provides a method for distributing liquid by using the liquid distribution device, the method comprising: the liquid to be distributed is transported to the liquid storage cavity 22 by the liquid injection component 1, the liquid is quantitatively distributed and stored in the liquid storage cavity 22, the liquid storage cavity 22 is separated from the playback liquid component 23 by applying an external force, and the stored liquid flows downward along the outer wall of the playback liquid component 23 and flows to the liquid transfer plate 4; the remaining liquid in the liquid storage cavity 22 is discharged through the liquid discharge component 3.

[0034] According to the liquid distribution method of the application, the remaining liquid in the liquid storage cavity 22 can be discharged through the liquid discharge component 3 after the liquid distribution is completed.

[0035] According to the liquid distribution method of the application, preferably, the external force is selected from a mechanical external force, a gas-closed external force or an automatic external force. The automatic external force can be an external force controlled by a PLC, a DCS or the like operating system, and considering the cost, the mechanical external force or the gas-closed external force is preferred. For example, the external force can be a manual external force.

[0036] According to one specific embodiment of the present application, the method for liquid dispensing includes, but is not limited to, pouring the liquid to be dispensed into the liquid injection component 1, opening the partition between the liquid injection component 1 and the liquid distribution cavity 22, and allowing the liquid to be dispensed to flow into the liquid distribution cavity 22. The liquid distribution cavity 22 is provided with a plurality of holes, each of which is provided with a boss 221, a flow groove 222, and a liquid storage component 223. When the liquid distribution cavity 22 and the playback liquid component 23 are in a closed state, the liquid to be dispensed flows into the holes through the flow grooves 222 and is quantitatively dispensed to form quantitatively dispensed liquid, which is stored in the space formed by the liquid storage component 223 and the playback liquid component 23. The spring 24 supports the pressing plate 21, and an external force presses the pressing plate 21, which is in contact with the tube body 231, downward, and the playback liquid component 23 as a whole is also downward. At this time, the liquid distribution cavity 22 and the playback liquid component 23 are separated, and the liquid stored in the space formed by the liquid storage component 223 and the playback liquid component 23 flows downward along the outer wall of the conical component 233 and flows to the pipette plate 4. At the same time, the liquid locking component 232 of the playback liquid component 23 is in sealed connection with the boss 221 to prevent the liquid to be dispensed from flowing into the holes, thereby ensuring the quantitative and uniform distribution of the liquid. When the external force is removed, the spring 24 is lifted, the tube body 231 in contact with the pressing plate 21 is upward, and the playback liquid component 23 penetrating the liquid distribution cavity 22 as a whole is also upward, so that the liquid distribution cavity 22 and the playback liquid component 23 are closed, and the liquid to be dispensed flows into the holes through the flow grooves 222, and the quantitative dispensing of the liquid is completed and stored in the space formed by the liquid storage component 223 and the playback liquid component 23. When the pipette plate 4 is replaced and the external force is applied to press the pressing plate 21 again, the spring is compressed, the tube body 231 in contact with the pressing plate 21 is downward, and the liquid distribution cavity 22 and the playback liquid component 23 are separated. The liquid stored in the space formed by the liquid storage component 223 and the playback liquid component 23 flows downward along the outer wall of the conical component 233 and flows to the pipette plate 4. At the same time, the liquid locking component 232 of the playback liquid component 23 is in sealed connection with the boss 221 to prevent the liquid to be dispensed from flowing into the holes, thereby ensuring the quantitative and uniform distribution of the liquid. According to the need, the cycle is repeated several times, so that the liquid dispensing device of the present application can continuously dispense liquid.

[0037] The third aspect of the present application provides the above-mentioned liquid dispensing device or the above-mentioned method for use in biology and chemistry.

[0038] The present application has at least one of the following advantages:

[0039] 1. The existing automatic multi-channel pipetting device has a high cost, about 100,000 yuan per device, and also needs to use the gun head and the corresponding supporting device of the manufacturer. Once the related parts inside the device have problems and affect the use, the cost and processing fee of the device are relatively high. The device of the present application can greatly reduce the cost of the device, and the cost is only thousands of yuan, and the cost of the parts is low, and the replacement is convenient.

[0040] 2、The liquid dispensing device and method of the present application can continuously pipette, equally distribute liquid, have the advantages of simple operation, time and labor saving, and can greatly save operation cost.

[0041] Additional aspects and advantages of the present application will be described in the description that follows, and in part will become apparent to those skilled in the art from the description, or by practice of the application. BRIEF DESCRIPTION OF DRAWINGS

[0042] Figure 1 The schematic diagram of the liquid dispensing device of the embodiment 1 of the present application is split;

[0043] Figure 2 The schematic diagram of the liquid dispensing device of the embodiment 1 of the present application is assembled;

[0044] Figure 3 The cross-sectional view of the playback liquid component of the liquid dispensing device of the embodiment 1 of the present application is shown;

[0045] Figure 4 The cross-sectional view of the liquid distribution cavity of the liquid dispensing device of the embodiment 1 of the present application is shown;

[0046] Figure 5 The bottom plate schematic diagram of the liquid distribution cavity of the liquid dispensing device of the embodiment 1 of the present application is shown.

[0047] Wherein, 1-liquid injection component; 2-main body; 21-pressing plate; 22-liquid distribution cavity; 23-playback liquid component; 24-spring; 221-convex boss; 222-flow channel; 223-liquid storage component; 231-tube body; 232-liquid locking component; 233-conical component; 234-sealing ring; 3-liquid discharge component; 31-sealing plug; 4-pipetting plate; 5-liquid storage container. DETAILED DESCRIPTION

[0048] The present application will be described below with reference to specific embodiments, and it should be noted that these embodiments are merely illustrative and should not be construed as limiting the present application.

[0049] The specific embodiments of the present application will be further described below with reference to the accompanying drawings. Wherein the same parts are denoted by the same reference numerals. It should be noted that the words "front", "back", "left", "right", "up" and "down" used in the following description refer to the directions in the drawings, and the words "inner" and "outer" refer to the directions towards or away from the geometric center of a particular component.

[0050] Embodiment 1

[0051] Adopted Figures 1-5The liquid dispensing device is shown, the device has sequentially communicated liquid injection component 1, main body 2 and liquid discharge component 3, the main body 2 has the pressing plate 21, the liquid separation cavity 22 and the playback liquid component 23 which are arranged from top to bottom, wherein the liquid injection component 1 is used for conveying the liquid to be dispensed to the liquid separation cavity 22; the liquid separation cavity 22 and the playback liquid component 23 are sealable or separable, when the liquid separation cavity 22 and the playback liquid component 23 are sealed, the liquid to be dispensed is quantitatively dispensed and the quantitatively dispensed liquid is stored; the pressing plate 21 is used for separating the liquid separation cavity 22 and the playback liquid component 23 by applying external force, the stored quantitatively dispensed liquid flows along the outer wall of the playback liquid component 23 and flows to the liquid transfer plate 4; the liquid discharge component 3 is used for discharging the remaining liquid in the liquid separation cavity 22;

[0052] Wherein the bottom plate of the liquid separation cavity 22 is arranged in a layout of high in the middle and low at both ends in the direction perpendicular to the liquid injection component 1 and the liquid discharge component 3 (such as Figure 5 The bottom plate of the liquid separation cavity 22 is provided with a flow guide groove, the flow guide groove is communicated with the liquid injection component 1 and the liquid discharge component 3 respectively at both ends, and the height of the liquid injection component 1 is higher than that of the liquid discharge component 3, the flow guide groove is arranged in a slope α in the horizontal direction, and α is 5°;

[0053] Wherein the liquid separation cavity 22 is provided with 96 holes, each hole is provided with a boss 221, a flow groove 222 and a liquid storage component 223; the boss 221 protrudes from the hole of the hole, which is used to prevent the liquid to be dispensed from flowing into the hole; the flow groove 222 is arranged on the boss 221, which is used to make the liquid to be dispensed flow into the hole for quantitative dispensing to form quantitatively dispensed liquid; when the liquid separation cavity 22 and the playback liquid component 23 are in a sealed state, the quantitatively dispensed liquid is stored in the space formed by the liquid storage component 223 and the playback liquid component 23 (the volume of the space formed by each liquid storage component 223 and the playback liquid component 23 is 250 μL);

[0054] Wherein the playback liquid component 23 has 96 pipe bodies 231 matched with the 96 holes of the liquid separation cavity 22, each pipe body 231 is provided with a liquid locking component 232 on the side wall, and each pipe body 231 is provided with a tapered component 233 at the lower end; the top of each pipe body 231 is in contact with the pressing plate 21; the liquid locking component 232 is used for sealing connection with the boss 221 and preventing the liquid to be dispensed from flowing into the hole; the liquid locking component 232 is provided with an exhaust hole, and the inside of the liquid locking component 232 is provided with a sealing ring, and the outer wall of the tapered component 233 is provided with a sealing ring 234;

[0055] Wherein the liquid injection component 1 and the liquid separation cavity 22 of the main body 2 are provided with a partition plate; the liquid discharge component 3 and the liquid separation cavity 22 of the main body 2 are provided with a sealing plug 31; the liquid dispensing device further comprises a spring 24 for supporting the pressing plate 21.

[0056] Example 2

[0057] The liquid dispensing device of Example 1 is used to dispense liquid. The liquid to be dispensed in the liquid storage container 5 is poured into the liquid injection component 1. The partition between the liquid injection component 1 and the liquid distribution chamber 22 is opened. The liquid to be dispensed flows into the liquid distribution chamber 22. When the liquid distribution chamber 22 and the liquid playback component 23 are in a sealed state, the liquid to be dispensed flows into the hole through the flow channel 222 in the liquid distribution chamber 22 and is quantitatively dispensed. The quantitatively dispensed liquid is stored in the space formed by the liquid storage component 223 and the liquid playback component 23 (the volume of the space formed by each of the liquid storage component 223 and the liquid playback component 23 is 250 μL). The spring 24 supports the pressing plate 21. An external force is manually applied to press the pressing plate 21. The tube body 231 in contact with the pressing plate 21 moves downward. The liquid playback component 23 as a whole moves downward. At this time, the liquid distribution chamber 22 and the liquid playback component 23 are separated. The liquid stored in the space formed by the liquid storage component 223 and the liquid playback component 23 flows downward along the outer wall of the conical component 233 and flows to the liquid transfer plate 4 (96-well plate). At the same time, the liquid locking component 232 of the liquid playback component 23 is in sealing connection with the boss 221 to prevent the liquid to be dispensed from flowing into the hole and to ensure quantitative and uniform liquid distribution. When the external force is manually removed, the spring 24 is released. The tube body 231 in contact with the pressing plate 21 moves upward. The liquid playback component 23 penetrating the liquid distribution chamber 22 as a whole moves upward. The liquid distribution chamber 22 and the liquid playback component 23 are in a sealed state. The liquid to be dispensed flows into the hole through the flow channel 222. The quantitative dispensing of the liquid is completed. The liquid is stored in the space formed by the liquid storage component 223 and the liquid playback component 23.

[0058] The liquid transfer plate 4 (96-well plate) is replaced. When the external force is manually applied to press the pressing plate 21 again, the spring is compressed. The tube body 231 in contact with the pressing plate 21 moves downward. The liquid distribution chamber 22 and the liquid playback component 23 are separated. The liquid stored in the space formed by the liquid storage component 223 and the liquid playback component 23 flows downward along the outer wall of the conical component 233 and flows to the liquid transfer plate 4. At the same time, the liquid locking component 232 of the liquid playback component 23 is in sealing connection with the boss 221 to prevent the liquid to be dispensed from flowing into the hole and to ensure quantitative and uniform liquid distribution.

[0059] The external force is manually applied to press the pressing plate 10 times. The liquid dispensing of 10 96-well plates is completed. After the liquid dispensing is completed, the remaining liquid in the liquid distribution chamber 22 is discharged through the liquid discharge component 3.

[0060] The liquid dispensing device of the present application can continuously dispense liquid. The volume of the liquid in any 10 wells of the liquid transfer plate 4 (96-well plate) is measured by using a liquid transfer gun. The volume is 250 μL. It is proved that the liquid dispensing device of the present application can accurately and quantitatively dispense liquid.

[0061] In the description of the specification, reference to "one embodiment", "some embodiments", "an example", "a specific example", or "some examples" means that a particular feature, structure, material, or characteristic being described is included in at least one embodiment or example of the application. The appearances of the above expressions in various places in the specification are not necessarily referring to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics can be combined in any suitable manner in one or more embodiments or examples.

[0062] Although embodiments of the application have been shown and described, it will be understood by those of ordinary skill in the art that various changes, modifications, substitutions, and alterations can be made hereto without departing from the spirit and scope of the application, which is defined by the following claims and their equivalents.

Claims

1. A liquid dispensing device, characterized in that, The device includes an injection component (1), a main body (2), and a drainage component (3) connected in sequence. The main body (2) includes a pressure plate (21) arranged from top to bottom, a liquid distribution chamber (22), and a return liquid component (23) penetrating the liquid distribution chamber (22). The liquid injection component (1) is used to deliver the liquid to be dispensed to the dispensing chamber (22); The liquid dispensing chamber (22) and the liquid return component (23) can be sealed or separated. When the liquid dispensing chamber (22) and the liquid return component (23) are sealed, they are used to quantitatively dispense the liquid to be dispensed and store the quantitatively dispensed liquid. The pressure plate (21) is used to separate the liquid dispensing chamber (22) from the liquid return component (23) by applying external force, and the stored quantitatively dispensed liquid flows down along the outer wall of the liquid return component (23) and flows to the pipette plate (4); The draining component (3) is used to drain the remaining liquid from the liquid distribution chamber (22); The liquid separation chamber (22) is provided with multiple holes, each hole including a boss (221), a flow groove (222) and a liquid storage component (223); The boss (221) protrudes from the opening of the hole to prevent the liquid to be dispensed from flowing into the hole; The flow groove (222) is provided on the boss (221) to allow the liquid to be distributed to flow into the hole for quantitative distribution to form quantitatively distributed liquid; When the liquid separation chamber (22) and the liquid return component (23) are in a closed state, the quantitatively distributed liquid is stored in the space formed by the liquid storage component (223) and the liquid return component (23); The liquid return component (23) includes multiple tubes (231) that match the multiple holes of the liquid distribution chamber (22), each tube (231) has a liquid-locking component (232) on its side wall, and each tube (231) has a tapered component (233) at its lower end; The top of each tube (231) is in contact with the pressure plate (21); The liquid-locking component (232) is used to seal and connect with the boss (221) and prevent the liquid to be dispensed from flowing into the hole; The diameter of the liquid-locking component (232) is larger than the diameter of the boss (221); The liquid-locking component (232) is provided with an exhaust port; The liquid-locking component (232) is provided with a sealing ring inside; A sealing ring (234) is provided on the outer wall of the tapered component (233).

2. The liquid dispensing device according to claim 1, characterized in that, The bottom plate of the liquid distribution chamber (22) is arranged with high ends and low middle along the direction perpendicular to the liquid injection component (1) and the liquid discharge component (3).

3. The liquid dispensing device according to claim 1 or 2, characterized in that, The bottom plate of the liquid separation chamber (22) is provided with a guide groove. The two ends of the guide groove are respectively connected to the liquid injection component (1) and the liquid drainage component (3), and the height of the liquid injection component (1) is higher than the height of the liquid drainage component (3).

4. The liquid dispensing device according to claim 3, characterized in that, The guide channel is arranged with a slope α along the horizontal direction.

5. The liquid dispensing device according to claim 4, characterized in that, The α angle is 1-60°.

6. The liquid dispensing device according to claim 5, characterized in that, The α angle is 5-45°.

7. The liquid dispensing device according to claim 6, characterized in that, The α angle is 5-30°.

8. The liquid dispensing apparatus according to any one of claims 1-3, characterized in that, A partition is provided between the liquid injection component (1) and the liquid distribution chamber (22) of the main body (2).

9. The liquid dispensing apparatus according to any one of claims 1-3, characterized in that, A sealing plug (31) is provided between the draining component (3) and the liquid distribution chamber (22) of the main body (2).

10. The liquid dispensing apparatus according to any one of claims 1-3, characterized in that, The liquid dispensing device also includes a spring (24) for supporting the pressure plate (21).

11. A method for liquid dispensing using the liquid dispensing apparatus according to any one of claims 1-10, characterized in that, The method includes: delivering the liquid to be dispensed to the dispensing chamber (22) through the injection component (1); when the dispensing chamber (22) and the return liquid component (23) are sealed, quantitatively dispensing the liquid to be dispensed and storing the quantitatively dispensed liquid; separating the dispensing chamber (22) and the return liquid component (23) by applying external force; the stored quantitatively dispensed liquid flows down along the outer wall of the return liquid component (23) and flows to the pipette plate (4); the remaining liquid in the dispensing chamber (22) is discharged through the drain component (3).

12. The application of the liquid dispensing apparatus according to any one of claims 1-10 or the method according to claim 11 in biology and chemistry.

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