A composition for improving skin microbiota comprising ginseng extract

Abstract

The present invention provides a composition for improving skin microbial flora, which includes a ginseng extract; an antibacterial agent derived from ginseng; and an alkanediol. The composition for improving skin microbial flora of the present invention can increase the proportion of beneficial bacteria in the skin microbial flora by promoting the proliferation of beneficial bacteria and inhibiting the proliferation of harmful bacteria, thereby improving the composition of the skin microbial flora to maintain a balanced state, ultimately contributing to the enhancement of the skin health of the human body.

Description

TECHNICAL FIELD

[0001] The present application relates to a composition for improving skin microbiota, comprising ginseng extract. BACKGROUND

[0002] Microbiome, a compound word from the combination of the words microflora and genome, refers to a microbial community, including all microorganisms and their genetic materials present in or coexisting in all environments such as humans, animals, and plants, soil, ocean, lake, rock, atmosphere, etc. Recently, microbiome has also been used to refer to a microbial community in various environments. Various microbiomes, such as plant microbiome, animal microbiome, environmental microbiome, human microbiome, gut microbiome, skin microbiome, etc., have been studied. Among these microbiomes, the human microbiome has been most actively studied.

[0003] The human body is a complex and active ecosystem, and various microorganisms exist in the intestines, skin, etc. of the human body. 500 to 1,000 different types of microorganisms symbiotically reside inside the intestines, skin, etc., forming a microbial flora. Intestinal microorganisms found in the intestines synthesize substances such as vitamins and provide them to the human body, and also play a role in preventing infection caused by pathogenic microorganisms introduced through contaminated food.

[0004] The microbial flora residing on the surface of the skin affects the physiological functions of humans, such as growth, nutrition, immunity, and resistance to pathogens, while also acting as a barrier to prevent the invasion of foreign microorganisms, particularly pathogenic microorganisms. In addition, the resident microbial flora of the skin plays a role in regulating the pH of the skin and acts as a skin barrier, and is also known to affect the state of the skin through changes in the content of skin lipids.

[0005] The skin microbial flora not only contains beneficial microorganisms, but also contains harmful microorganisms that are harmful to the human body. Therefore, for healthy skin, it is necessary to promote the growth of beneficial microorganisms and inhibit the growth of harmful microorganisms.

[0006] Ginseng (Panax ginseng C.A. Meyer), a perennial herb of the genus Panax of the Araliaceae family, has been traditionally used in Oriental medicine, which is sweet and bitter in taste, has a warming and invigorating energy, and has herbal effects such as strengthening vital energy, preserving the spleen, and invigorating the lungs.

[0007] The main bioactive substances of ginseng include saponins, also called ginsenosides, and essential oil components, polyacetylenes, phenolic components, glycosides, acidic peptides, etc., and also include other components such as vitamins, sugars, and minerals. As general bioactive effects of ginseng, effects on the central nervous system, enhancement of immune function, and anticancer effects, etc. have been reported. In particular, about 40 types of ginsenosides have been discovered to date, and such ginsenosides have been found to have a wide range of effects on the endocrine system, the immune system, and the metabolic system, as well as the central nervous system, and thus, have proven to have an ideal effect in regulating body functions, i.e., normalizing physiological functions. These ginsenosides can have similar activities to each other or opposite activities to each other, and it is known that a specific ginsenoside alone or multiple types of ginsenosides together achieve various effects through interactions.

[0008] The present inventors found that a ginseng extract promotes the growth of beneficial microorganisms, and found the potential use of the ginseng extract as a skin prebiotic. In this context, the present inventors researched a composition capable of selectively increasing beneficial microorganisms alone while inhibiting the growth of harmful microorganisms, and as a result, found that the use of a ginseng extract in combination with an antibacterial agent derived from ginseng and hexanediol can effectively inhibit the growth of harmful microorganisms while promoting the growth of beneficial microorganisms, thereby completing the present invention. SUMMARY

[0009] PROBLEM TO BE SOLVED BY THE INVENTION

[0010] An object of the present invention is to provide a composition for improving a skin microbial flora, which is capable of promoting the growth of beneficial microorganisms in the skin microbial flora while inhibiting the growth of harmful microorganisms.

[0011] TECHNICAL SOLUTION

[0012] One aspect of the present invention provides a composition for improving a skin microbial flora, the composition comprising: a ginseng extract; an antibacterial agent derived from ginseng; and an alkanediol.

[0013] ADVANTAGEOUS EFFECTS

[0014] The composition for improving a skin microbial flora of the present invention can increase the proportion of beneficial microorganisms in the skin microbial flora by promoting the growth of beneficial microorganisms while inhibiting the growth of harmful microorganisms. Accordingly, the composition of the present invention can improve the composition of the skin microbial flora and maintain it in a balanced state, and thus can ultimately provide the benefit of enhancing human skin health.

[0015] In particular, the composition of the present invention can exhibit an effect of delaying the rate of skin aging by increasing the proportion of beneficial microorganisms in the skin microbial flora, or an effect of relieving skin conditions by reducing the proportion of harmful microorganisms that have an adverse effect on the skin.

[0016] In addition, the composition for improving skin microbial flora of the present application contains ginseng concentrate and an antibacterial agent derived from ginseng as main ingredients, and exhibits a desirable effect of promoting the growth of beneficial microorganisms with respect to harmful microorganisms even at a low concentration of alkylene glycol, and thus has the advantages that the composition can exhibit low toxicity, less irritation to the skin, and desirable stability. BRIEF DESCRIPTION OF DRAWINGS

[0017] FIG. 1 is a graph comparing the promotion of the growth of skin beneficial microorganisms by fructo-oligosaccharides and red ginseng concentrate. DETAILED DESCRIPTION

[0018] Hereinafter, the present application will be described in more detail.

[0019] The terms and words used in the present specification and claims should not be limited to the meanings that are commonly used and dictionary meanings, but should be interpreted based on the principle that the inventor appropriately defines the terms to best describe the present application in accordance with the technical spirit of the present application.

[0020] In the present application, the term "microbial flora" refers to a microbial community, including bacteria, fungi, and other prokaryotes found in the human body, which generally inhabit the intestines, skin, etc.

[0021] In the present application, the term "skin microbial flora" refers to a microbial community found in the skin, most of which exists on the surface of the skin, such as the scalp, pores, etc. The skin microbial flora contains beneficial skin microorganisms that exhibit a beneficial effect on the skin, such as maintaining moisture in the skin, eliminating reactive oxygen species, etc., and also contains harmful skin microorganisms that cause skin problems such as acne and abscesses.

[0022] Examples of beneficial skin microorganisms include, but are not limited to, Staphylococcus strains such as Staphylococcus epidermidis, Streptococcus muris strains such as Streptococcus pneumoniae, and Lactobacillus strains such as Lactobacillus acidophilus, Lactobacillus rhamnosus, and Bifidobacterium bifidum, etc.

[0023] In particular, Staphylococcus epidermidis is a gram-positive bacterium commonly found on the skin, mucous membranes, etc. of humans and animals. It is known that Staphylococcus epidermidis produces glycerol to enhance skin moisturization (J Invest Dermatol, 1960, 34: 171-174), synthesizes organic acids to maintain acidic conditions on the skin (Br J Dermatol 1986, 80: 279-281), and produces antibiotic substances to inhibit the growth of harmful microorganisms such as Staphylococcus aureus on the skin (N Engl J Med, 1998, 339: 520-532; Nature, 2010, 465: 346-349; Peptides, 2010, 31: 1661-1668; J Invest Dermatol, 2010, 130: 192-200), thus showing a beneficial effect on the skin.

[0024] Examples of harmful skin microorganisms include, but are not limited to, Staphylococcus aureus, Propionibacterium acnes, etc.

[0025] In particular, Staphylococcus aureus is a gram-positive facultative anaerobe that is usually found in the inner layer of the skin or nasal cavity of healthy humans and animals. It is known that Staphylococcus aureus is a pathogenic bacterium of food-borne diseases and suppurative diseases such as skin abscess, middle ear inflammation, bladder inflammation, etc.

[0026] Propionibacterium acnes secretes lipase that decomposes triglycerides in sebum to form free fatty acids and stimulate hair follicles. In addition, it is known that an immune response to this bacterium, etc. is associated with the formation of acne.

[0027] In the present application, the term "improvement" refers to a state in which the composition of a microbial flora is changed in a direction beneficial to the human body due to the administration of a composition. The term can specifically mean promoting the growth of beneficial microorganisms inherent to the skin while inhibiting harmful microorganisms, thereby increasing the proportion of beneficial microorganisms in the skin microbial flora.

[0028] One aspect of the present application provides a composition for improving a skin microbial flora, the composition comprising: a ginseng extract; an antibacterial agent derived from ginseng; and an alkanediol.

[0029] The composition for improving a skin microbial flora of the present application can promote the growth of beneficial microorganisms while inhibiting the growth of harmful microorganisms. An increase in the proportion of beneficial microorganisms in the microbial flora can be used to prevent pathogenic microorganism infection, enhance immunity, enhance skin health, and reduce body odor.

[0030] The ginseng extract can be obtained from ginseng by known techniques. For the ginseng, any ginseng of cultivation or on the market can be used without limitation. The ginseng can include various processing types, and can be, for example, at least one selected from the group consisting of fresh ginseng, ginseng rootlet, black ginseng, wild ginseng, cultivated wild ginseng, mountain ginseng, dextrinized ginseng, enzyme-treated ginseng rootlet, fermented ginseng, red ginseng, and fermented red ginseng, but is not limited thereto. The fresh ginseng refers to the raw ginseng which maintains its original form, and the ginseng rootlet refers to the fine root of ginseng. The red ginseng is prepared by steaming and drying the raw ginseng, and shows yellowish brown to reddish brown due to the browning reaction during the drying process. The black ginseng refers to the ginseng prepared by steaming and drying the red ginseng several times, which becomes black in color. The wild ginseng refers to the naturally grown ginseng, and the cultivated wild ginseng refers to the wild ginseng which is planted and cultivated. The mountain ginseng refers to the ginseng cultivated by artificially sowing or transplanting ginseng seedlings in the forest land of the mountain.

[0031] As an extraction method for obtaining the extract from the above-mentioned ginseng, any conventional and known extraction method such as solvent extraction, ultrasonic extraction, filtration, reflux extraction, etc. can be used, and preferably, the extract can be prepared using the solvent extraction or the reflux extraction. Such an extraction process can be repeated several times, and then additional steps such as concentration and freeze-drying can be performed. In particular, the extract thus obtained can be concentrated under reduced pressure to produce a concentrate. The concentrate can be freeze-dried, and using a grinder, a high-concentration extract powder can be prepared therefrom. The extract includes fractions obtained by further fractionating the extract.

[0032] When the extract is obtained from ginseng, one or more selected from the group consisting of water, an organic solvent, a supercritical fluid, and a mixture thereof can be used as an extraction solvent. The organic solvent can be any one selected from the group consisting of an alcohol of a lower alcohol of preferably C1-C4, hexane (n-hexane), an ether, glycerol, propylene glycol, butylene glycol, ethyl acetate, methyl acetate, dichloromethane, chloroform, ethyl acetate, benzene, and a mixed solvent thereof, and can be preferably ethanol. For example, when a mixture of water and an organic solvent is used as the extraction solvent, the mixture of water and the organic solvent can be preferably a mixture of water and a lower alcohol of C1-C4, more preferably a mixture of water and ethanol.

[0033] In the present application, the ginseng-derived antibacterial agent can be a purified fraction of the ginseng extract of ethanol or an aqueous ethanol solution using a column. The fraction can be, but is not limited to, in the form of a solid powder obtained by performing additional steps such as concentration and / or freeze-drying, etc.

[0034] As for the preparation of the ginseng-derived antibacterial agent, details regarding the ginseng and the method of obtaining the extract therefrom can be the same as described above, and thus will not be further described in detail.

[0035] The aqueous ethanol solution used to prepare the ginseng-derived antibacterial agent can be at least 50% (v / v) and less than 100% (v / v), and can be 50 to 95% (v / v), 50 to 90% (v / v), and 50 to 85% (v / v), but is not limited thereto. When the concentration of the aqueous ethanol solution is less than 50% (v / v), the antibacterial effect can be relatively low compared to when extraction is performed with an appropriate ethanol content.

[0036] As the stationary phase used in the preparation of the purified fraction, a filler such as silica gel, activated alumina, a synthetic polymer, magnesium silicate, activated carbon, cellulose, an ion exchange polymer, etc. can be used, and silica gel or a synthetic aromatic polymer is preferably used as the filler. When a synthetic aromatic polymer is used, the column can be an aromatic synthetic adsorption column, and the filler is preferably Diaion HP-20 synthetic adsorbent, but is not limited thereto. Separation using such a column can be performed once to several times until a fraction of the desired purity is obtained, and, as necessary, concentration and recrystallization can be performed.

[0037] The desorption solvent used to prepare the above-described purified fraction can be any one selected from the group consisting of water, an organic solvent, and a mixture thereof. The organic solvent can be any one selected from the group consisting of an alcohol, hexane (n-hexane), an ether, glycerol, propylene glycol, butylene glycol, ethyl acetate, methyl acetate, dichloromethane, chloroform, ethyl acetate, benzene, acetone, acetonitrile, and a mixed solvent thereof. The organic solvent is preferably any one selected from the group consisting of acetone, acetonitrile, a C1-C4 alcohol, and a mixed solvent thereof. The organic solvent can be more preferably ethanol, but is not limited thereto.

[0038] When the desorption solvent used to prepare the purified fraction is ethanol, the desorption solvent can be ethanol or an aqueous ethanol solution, and the desorption solvent can be, but is not limited to, an aqueous ethanol solution of at least 50% (v / v) and less than 100% (v / v), preferably an aqueous ethanol solution of 50 to 99% (v / v), an aqueous ethanol solution of 70 to 97% (v / v), or an aqueous ethanol solution of 85 to 95% (v / v). When the concentration of the aqueous ethanol solution used as the desorption solvent is lower than the lower limit value, the desorption of the active ingredient obtained can occur to an insufficient degree, thereby reducing the antibacterial effect of the fraction.

[0039] When the purified fraction is prepared, the addition of the aqueous ethanol solution as the desorption solvent can be completed by passing the aqueous ethanol solution through a column, for example, by passing the aqueous ethanol solution through a column under pressure.

[0040] The alkylene glycol used in the present application can be a colorless and odorless liquid and has a purity of 99.5% or more. In particular, the alkylene glycol can be one or more selected from the group consisting of 1,2-butanediol, 1,3-butanediol, 1,4-butanediol, 1,5-pentanediol, neopentyl glycol, isopentyl glycol (3-methyl-1,3-butanediol), 1,2-hexanediol, 1,6-hexanediol, 3-methyl-1,5-pentanediol, 1,2-octanediol, 2,3-octanediol, 2-ethyl-1,3-hexanediol, 2-butyl-2-ethyl-1,3-propanediol, 2,5-dimethyl-2,5-hexanediol, 1,2-decanediol, 1,2-dodecanediol, 1,2-tetradecanediol, 1,2-hexadecanediol, 1,2-octadecanediol, 1,12-octadecanediol, 1,2-cyclohexanediol, 1,4-cyclohexanediol, and 1,4-cyclohexanedimethanol, and can be preferably 1,2-hexanediol.

[0041] The composition for improving skin microbial flora of the present application can include 0.05 to 8 parts by weight of ginseng extract; 0.01 to 1 part by weight of an antibacterial agent derived from ginseng; and 0.1 to 10 parts by weight of an alkylene glycol.

[0042] For example, the ginseng extract can be 0.05 to 7 parts by weight, 0.07 to 5 parts by weight, 0.1 to 3 parts by weight, or 0.1 to 1 part by weight, the antibacterial agent derived from ginseng can be 0.03 to 0.7 parts by weight, 0.05 to 0.5 parts by weight, or 0.1 to 0.3 parts by weight, and the alkylene glycol can be 0.1 to 7 parts by weight, 0.3 to 5 parts by weight, 0.5 to 3 parts by weight, or 0.5 to 1.5 parts by weight.

[0043] When included in such amounts, the effect of promoting the growth of beneficial microorganisms and inhibiting the growth of harmful microorganisms can be desirably high, thus significantly increasing the increase of beneficial microorganisms relative to harmful microorganisms.

[0044] The composition for improving skin microbial flora of the present application can be a cosmetic composition.

[0045] The cosmetic composition of the present application can be prepared in a liquid or solid form using bases, adjuvants, and additives commonly used in the cosmetic industry. Examples of such cosmetics in a liquid or solid form can include cosmetic water, cream formulations, emulsion formulations, bath formulations, etc., but are not limited thereto. The bases, adjuvants, and additives commonly used in the cosmetic industry are not particularly limited, and can include, for example, water, alcohol, propylene glycol, stearic acid, glycerin, cetyl alcohol, liquid paraffin, etc.

[0046] The cosmetic composition of the present application can include ingredients commonly used in cosmetic compositions, and for example, can include carriers and common adjuvants such as antioxidants, stabilizers, solubilizers, vitamins, pigments, and fragrances.

[0047] The cosmetic composition of the present application can be prepared in any formulation generally produced in the related technical field, and for example, can be formulated as, but not limited to, a solution, a suspension, an emulsion, a paste, a gel, a cream, a lotion, a powder, a soap, a surfactant-containing cleanser, an oil, a microcapsule, a microparticle, an ionic vesicular dispersion, a non-ionic vesicular dispersion, a powder foundation, an emulsion foundation, a wax foundation, a stick, a pack, a spray, etc. More specifically, the cosmetic composition can be prepared into a formulation such as a skin-moisturizing toner, a nutrition toner, a nutrition cream, a massage cream, an essence, an eye cream, a cleansing paste, a cleansing foam, a cleansing water, a pack, a spray, and a powder.

[0048] The cosmetic composition of the present application, when its formulation is a paste, a cream, or a gel, can use an animal oil, a vegetable oil, a wax, a paraffin, a starch, a tragacanth gum, a cellulose derivative, a polyethylene glycol, a silicone, a bentonite, a silicon dioxide, a talc, a zinc oxide, etc., as a carrier component.

[0049] The cosmetic composition of the present application, when its formulation is a powder or a spray, can use a lactose, a talc, a silicon dioxide, an aluminum hydroxide, a calcium silicate, or a polyamide powder, as a carrier component. In particular, the cosmetic composition, when its formulation is a spray, can further include a propellant such as a chlorofluorocarbon, a propane / butane, and a dimethyl ether.

[0050] The cosmetic composition of the present application, when its formulation is a solution or an emulsion, can use a solvent, a solubilizer, or an emulsifier as a carrier component, for example, water, ethanol, isopropyl alcohol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1,3-butylene glycol oil, glycerin fatty acid ester, polyethylene glycol, and sorbitan fatty acid ester.

[0051] The cosmetic composition of the present application, when its formulation is a suspension, can use a liquid diluent such as water, ethanol, and propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and, polyoxyethylene sorbitan ester, or microcrystalline cellulose, methyl aluminum hydroxide, bentonite, agar, or tragacanth gum, etc., as a carrier component.

[0052] The cosmetic composition of the present application, when its formulation is a surfactant-containing cleanser, can use an aliphatic alcohol sulfate, an aliphatic alcohol ether sulfate, a sulfosuccinic acid monoester, an imidazoline derivative, a methyl taurate, a sarcosinate, a fatty acid amide ether sulfate, an alkyl amide betaine, an aliphatic alcohol, a fatty acid glyceride, a fatty acid diethanolamide, a vegetable oil, a lanolin derivative, an ethoxylated glycerin fatty acid ester, etc., as a carrier component.

[0053] The cosmetic composition of the present application can be used in a single application or repeated applications, or can be used in repeated applications together with other cosmetic compositions other than the present application. In addition, the cosmetic composition of the present application can be used by a conventional use method, and can be used at a frequency varied according to the skin condition or preference of the user.

[0054] When the formulation of the cosmetic composition of the present application is a soap, a surfactant-containing cleanser, or a surfactant-free cleanser, it can be wiped off, separated, or rinsed off with water after being applied to the skin. As specific examples, the soap can include, but is not limited to, a liquid soap, a powder soap, a solid soap, or an oil soap, the surfactant-containing cleanser formulation can be, but is not limited to, a cleansing foam, a cleansing solution, a cleansing cloth, and a cleansing pack, and the surfactant-free cleanser formulation can be, but is not limited to, a cleansing cream, a cleansing lotion, a cleansing solution, or a cleansing gel. DETAILED DESCRIPTION

[0056] Hereinafter, the present application will be described in more detail through Preparation Examples and Examples.

[0057] However, the following Preparation Examples and Examples are provided only for the purpose of illustrating the present application, and thus the scope of the present application should not be limited to the following Preparation Examples and Examples.

[0058] Example 1

[0059] 1-1. Preparation of Red Ginseng Concentrate

[0060] 1 kg of the six-year-old red ginseng was mixed with 6 times the volume of purified water and extracted at room temperature for 8 hours or more and then concentrated under reduced pressure to obtain a red ginseng concentrate.

[0061] 1-2. Preparation of Red Ginseng Anti-bacterial Powder

[0062] 1 kg of the red ginseng was mixed with 5.5 times the volume of 70% (v / v) ethanol and extracted at 70°C for 3 times for 8 hours. The resulting extract was treated with a rotary evaporator to obtain 50 g or more of a red ginseng ethanol extract concentrate having a water content of 40%. The red ginseng ethanol extract concentrate was combined and diluted with 3 times the volume of purified water, and 150 g of the resulting diluent was passed through a column packed with 100 g of HP-20 ion exchange polymer. After washing the column with 5 times the volume of purified water, an ethanol aqueous solution (95% (v / v)) was passed through the column, and fractions of active ingredients attached to the ion exchange polymer column were collected. After completely evaporating the solvent from the fractions thus obtained, the fractions were combined with purified water and frozen at -70°C, and then dried in a freeze dryer to produce a solid.

[0063] 1-3. Preparation of Composition for Improving Skin Microbial Flora

[0064] 0.1 g of red ginseng concentrate and 0.01 g of red ginseng antibacterial powder were dissolved in 10 ml of saline solution, and then mixed with 0.1 ml of 1,2-hexanediol to prepare the composition for improving skin microbial flora of Example 1.

[0065] Comparative Examples 1 to 5

[0066] A composition for improving skin microflora was prepared by the same method as above, but the ingredients in the composition for improving skin microflora were changed, as shown in the following table.

[0067] [Table 1]

[0068] No. Blending Conditions Example 1 Red Ginseng Concentrate (10 mg / ml) + Red Ginseng Anti-bacterial Powder (1 mg / ml) + Hexylene Glycol (1%) Comparative Example 1 Red Ginseng Concentrate (10 mg / ml) Comparative Example 2 3-Sialyllactose (10 mg / ml) Comparative Example 3 6-Sialyllactose (10 mg / ml) Comparative Example 4 Red Ginseng Concentrate (10 mg / ml) + Anisic Acid (2 mg / ml) Comparative Example 5 Red Ginseng Concentrate (10 mg / ml) + Red Ginseng Anti-bacterial Powder (1 mg / ml)

[0069] Experimental Example 1

[0070] In order to compare the difference between oligofructose and the composition of the examples in promoting the growth of beneficial skin microorganisms, the samples were treated separately and the absorbance changes of the beneficial skin microorganisms were measured.

[0071] Specifically, the red ginseng concentrate of Production Example 1 and oligofructose (purity 95% or more) (purchased from Yongjin Pharmaceutical Co., Ltd.) were dissolved in a physiological saline solution at concentrations of 0.078 mg / ml, 0.156 mg / ml, 0.313 mg / ml, 0.625 mg / ml, 1.25 mg / ml, 2.5 mg / ml, 5 mg / ml, and 10 mg / ml, respectively.

[0072] The prepared samples were inoculated with Staphylococcus epidermidis (strain No. 1917, Korean Type Culture Collection) and cultured in a shaking incubator for 24 hours.

[0073] The solution in which the strain was grown was collected and centrifuged, and after removing the supernatant therefrom, an equal amount of saline solution was added to prepare a bacterial solution. Then, the absorbance at 600 nm was measured, and the results were as follows: FIG. 1 shown.

[0074] from FIG. 1 The results shown confirmed that the red ginseng concentrate had a very excellent growth-promoting effect even at a low concentration of 0.625 mg / ml, compared to oligofructose known as a prebiotic.

[0075] Experimental Example 2

[0076] In order to compare the differences between the compositions of Examples and Comparative Examples in terms of increased growth of beneficial skin microorganisms and harmful skin microorganisms, the growth of each microorganism was measured by the same method as described in Experimental Example 1.

[0077] Specifically, Staphylococcus epidermidis was used as a beneficial skin microorganism and Staphylococcus aureus (strain number 39149, Korean Collection for Type Cultures) was used as a harmful skin microorganism, and the samples were inoculated and cultured in a shaking incubator for 24 hours, respectively.

[0078] The solution in which the strains were grown was collected and centrifuged, and after removing the supernatant therefrom, an equal amount of a physiological saline solution was added to prepare a bacterial solution. Then, the absorbance at 600 nm was measured, and the results are shown in Table 2 below.

[0079] [Table 2]

[0080] No. OD600 (Beneficial Microorganism / Harmful Microorganism) Increase of Beneficial Microorganism to Harmful Microorganism Example 1 0.713 / 0.121 5.893 times Comparative Example 1 0.364 / 0.902 0.403 times Comparative Example 2 0.049 / 0.077 0.636 times Comparative Example 3 0.047 / 0.065 0.723 times Comparative Example 4 0.173 / 0.928 0.186 times Comparative Example 5 0.217 / 0.332 0.653 times

[0081] From the results shown in Table 2 above, it was found that the composition for improving skin microbial flora according to the present application selectively activates only beneficial microorganisms, not harmful microorganisms. It was also found that this selective growth promotion effect was significantly higher than when treated with red ginseng concentrate alone (Comparative Example 1) or with a combination of red ginseng concentrate and red ginseng antibacterial powder (Comparative Example 5).

[0082] In addition, when treated with sialyllactose, one of human milk oligosaccharides (HMOs) present in colostrum at the highest concentration compared to the composition according to the present application (Comparative Examples 2 and 3), the effect of promoting the growth of beneficial microorganisms was negligible, and the effect of selectively promoting only beneficial microorganisms was also low.

[0083] Likewise, when red ginseng concentrate was combined with anisic acid (Evonik Dr Straetmans GmbH, Germany) (Comparative Example 4), the effect of selectively promoting the growth of only beneficial microorganisms was negligible compared to the examples according to the present application.

[0084] Preparation of Example 1

[0085] 1-1. Preparation of serum

[0086] Using a mixture of red ginseng concentrate, red ginseng antibacterial powder, and hexylene glycol according to the present application, a serum was prepared in the amounts (parts by weight) shown in Table 3 below.

[0087] [Table 3]

[0088] Ingredient Content (weight parts) Triethanolamine 0.25 Carboxyvinyl polymer 0.22 Glycerin 4 Butylene glycol 2 Red Ginseng Concentrate of Example 1-1 5 Purified Fraction of Example 1-2 0.5 Hexylene glycol 1 Beeswax 0.5 Octadecanol 1 Glyceryl monostearate 1 Squalene 4 Purified water To 100

[0089] 1-2. Preparation of skin softening lotion

[0090] Using a mixture of red ginseng concentrate, red ginseng antibacterial powder, and hexylene glycol according to the present application, a skin softening lotion was prepared in the amounts (parts by weight) shown in Table 4 below.

[0091] [Table 4]

[0092] Ingredient Content (weight parts) 1,3-Butylene glycol 1.00 Disodium ethylenediaminetetraacetate 0.05 Allantoin 0.10 Dipotassium glycyrrhizate 0.05 Citric acid 0.01 Sodium citrate 0.02 Sugar ester-26 1.00 Arbutin 2.00 PEG-40 hydrogenated castor oil 1.00 Ethanol 30.00 Red Ginseng Concentrate of Example 1-1 5 Purified Fraction of Example 1-2 0.5 Hexylene glycol 1 Coloring agent Trace Fragrance Trace Purified water To 100

[0093] 1-3. Preparation of a nutritional cream

[0094] A nutritional cream was prepared using a mixture of the red ginseng concentrate, the red ginseng antibacterial powder, and hexylene glycol of the present application, according to the contents (weight parts) shown in Table 5 below.

[0095] [Table 5]

[0096]

[0097]

[0098] 1-4. Preparation of a lotion

[0099] A lotion was prepared using a mixture of the red ginseng concentrate, the red ginseng antibacterial powder, and hexylene glycol of the present application, according to the contents (weight parts) shown in Table 6 below.

[0100] [Table 6]

[0101]

[0102]

Claims

1. Use of a composition for improving skin microbial flora, the composition comprising: Ginseng extract; Ginseng-derived antimicrobial agent; and 1,2-hexanediol, wherein the ginseng of the ginseng extract and the ginseng-derived antibacterial agent is red ginseng, wherein the composition comprises: 0.05 to 8 parts by weight of the ginseng extract; 0.01 to 1 parts by weight of the ginseng-derived antibacterial agent; and 0.1 to 10 parts by weight of the 1,2-hexanediol. wherein said improving the skin microbial flora promotes the growth of beneficial skin microorganisms and inhibits the growth of harmful skin microorganisms, wherein the ginseng extract is extracted using one or more solvents selected from the group consisting of water and alcohols having 1-4 carbon atoms, and The ginseng-derived antibacterial agent is an extract of ginseng comprising ethanol or an aqueous ethanol solution having an ethanol content of at least 50% (v / v) and less than 100% (v / v), and a purified fraction obtained by using a column.

2. The method according to claim 1, wherein the stationary phase of the column is an aromatic synthetic polymer.

3. The method according to claim 1 , wherein the desorption solvent used to prepare the purified fraction is ethanol or an aqueous ethanol solution having an ethanol content of at least 50% (v / v) and less than 100% (v / v).

4. The method according to claim 1, wherein the beneficial skin microorganism is Staphylococcus epidermidis.

5. The method according to claim 1, wherein the harmful skin microorganism is Staphylococcus aureus.

6. The use according to claim 1, wherein the composition is a cosmetic composition.

7. The use according to claim 6, wherein the cosmetic composition is a formulation selected from the group consisting of solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, soaps, oils, and sticks.

8. The use according to claim 6, wherein the cosmetic composition is a preparation selected from the group consisting of a surfactant-containing cleanser, a powder foundation, an emulsion foundation, a wax foundation, and a facial mask.

9. The use according to claim 6, wherein the cosmetic composition is a preparation selected from the group consisting of microcapsules, microparticles, ionic vesicle dispersions, and non-ionic vesicle dispersions.

10. The use according to claim 6, wherein the cosmetic composition is a spray.

Images