A magnetic bead purification instrument

Abstract

The present invention belongs to the field of safety analysis and detection. The present invention discloses a magnetic bead purification instrument, including: a base, a lifting device, a translation device, a magnetic adsorption device and a control device; the magnetic adsorption device is used to adsorb and disperse magnetic beads, including a magnetic bar fixing component, a controllable magnetic bar, and a plurality of magnetic rods, and the magnetic bar fixing component also includes a magnetic bar cavity and a fixing groove; wherein, the controllable magnetic bar is arranged in the magnetic bar cavity of the magnetic bar fixing component, and the presence or absence of magnetism can be controlled by electric current; the magnetic rod is arranged in the fixing groove and is perpendicular to the controllable magnetic rod. In the present invention, an electromagnet is selected to replace a permanent magnet, and the presence or absence of magnetism is controlled by electric current, without the need to separately set up a magnetic bar sleeve and a corresponding control structure, thereby simplifying the magnetic adsorption device; all magnetic rods generate magnetism and demagnetize at the same time, thereby ensuring the consistency of the presence or absence of magnetism of all magnetic rods, and also reducing the number of magnetic rods.

Description

Technical Field

[0001] The invention relates to the field of safety analysis and detection, and in particular to a magnetic bead purification instrument. Background Art

[0002] Immunomagnetic beads are nanoscale materials formed by combining magnetic microsphere carriers and immune ligands. Magnetic microsphere carriers are usually magnetic beads with chemical functional groups such as amino, carboxyl, hydroxyl or thiol groups. The functional groups are combined with different immune ligands such as active proteins, antibodies, antigens, avidin, biotin, etc. to form immunomagnetic beads. Magnetic microsphere carriers have superparamagnetic characteristics, which can make the immunomagnetic beads show their magnetism when placed in a magnetic field. When removed from the magnetic field, the magnetism is eliminated and the immunomagnetic beads are redispersed. The antibody-coated immunomagnetic beads are used as solid phase carriers. The antibodies on them specifically bind to the corresponding antigens to form antigen-antibody-magnetic bead complexes. Under the action of the magnetic field, this complex quickly moves to the magnetic field, so that the complex is separated from other substances, achieving the purpose of separating specific antigens. This separation method has the advantages of fast detection speed, high specificity, high sensitivity, and good repeatability.

[0003] In order to realize the magnetic bead purification process, a magnetic bead extraction and purification instrument is usually used. The magnetic bead extraction and purification instrument in the prior art usually includes a plurality of magnetic extraction and separation devices including magnetic rods and magnetic rod sleeves arranged on a base, and a corresponding control system. Summary of the invention

[0004] The object of the present invention is to provide a magnetic bead purification instrument which has a simple structure, few parts, is easy to be handled in a portable manner and can reduce the control complexity.

[0005] To achieve the above purpose, the technical solution adopted by the present invention is as follows:

[0006] A magnetic bead purification instrument, used for removing impurities in a sample by an immunomagnetic bead method to improve the purity of the sample, comprising:

[0007] A base, used to carry and fix the components on the magnetic bead purification instrument;

[0008] A lifting device, disposed on the base, for achieving vertical movement;

[0009] A translation device, provided on the lifting device, for realizing movement in the horizontal direction;

[0010] Magnetic adsorption device, used to adsorb and disperse magnetic beads, comprising:

[0011] A magnetic bar fixing assembly, one end of which is connected to the translation device, comprises:

[0012] The magnetic rod cavity is arranged in the middle of the magnetic rod fixing assembly.

[0013] A fixing groove, provided at the bottom of the magnetic bar fixing assembly;

[0014] A controllable magnetic bar, which is arranged in the magnetic bar cavity of the magnetic bar fixing assembly and can control the presence or absence of magnetism through electric current;

[0015] A plurality of magnetic rods are arranged in the fixing groove and perpendicular to the controllable magnetic rod, and realize the presence or absence of magnetism under the control of the magnetic field generated by the controllable magnetic rod, and are used to contact the sample and adsorb and disperse the magnetic beads in the sample;

[0016] The control device is arranged on the base, is electrically connected to the lifting device and controls the lifting device to realize vertical movement, is electrically connected to the translation device and controls the translation device to realize horizontal movement, and is electrically connected to the controllable magnetic rod and controls the presence or absence of magnetism of the controllable magnetic rod.

[0017] Preferably, an oscillating device is provided in the magnetic bar fixing assembly, and the magnetic rod vibrates under the action of the oscillating device.

[0018] Preferably, the oscillating device is electrically connected to the control device, and the control device controls the movement of the oscillating device.

[0019] Preferably, the oscillation device is an ultrasonic oscillation device.

[0020] Preferably, a magnetic rod housing is provided outside the magnetic rod, the magnetic rod housing is an integrally formed conical tube, and the bottom end of the magnetic rod housing is a curved surface.

[0021] Preferably, the magnetic rod housing is a coating formed on the outer side of the magnetic rod by coating.

[0022] Preferably, the controllable magnetic rod comprises:

[0023] A magnetic bar core, made of soft iron or silicon steel, used to enhance the magnetic properties of the controllable magnetic bar;

[0024] The magnetic bar coil is spirally wound on the surface of the magnetic bar core and electrically connected to the control device, so as to endow the controllable magnetic bar with magnetism.

[0025] Preferably, the magnetic bead purification instrument further comprises:

[0026] A heating device is arranged on the base and is used for heating the sample.

[0027] Preferably, the magnetic bead purification instrument further comprises:

[0028] A housing, fixed on the base and forming a cavity, for accommodating the lifting device, the translation device, the magnetic adsorption device and the control device;

[0029] The human-machine interaction device is electrically connected to the control device and is used by an operator to control and monitor the operation of the magnetic bead purification instrument.

[0030] Preferably, a window is provided on the side of the shell for installing and unloading the sample.

[0031] Therefore, the present invention has at least the following beneficial effects:

[0032] (1) In the present invention, an electromagnet is used instead of a permanent magnet, and the presence or absence of magnetism is controlled by electric current, so there is no need to separately set up a magnetic rod sleeve and a corresponding control structure, thereby simplifying the magnetic adsorption device;

[0033] (2) In the present invention, all magnetic rods connected to the controllable magnetic rod are controlled by the controllable magnetic rod to generate magnetism and demagnetize at the same time, thereby ensuring the consistency of magnetism of all magnetic rods and reducing the number of magnetic rods. BRIEF DESCRIPTION OF THE DRAWINGS

[0034] Figure 1 A schematic diagram of the magnetic bead purification instrument in Example 1 of the present invention;

[0035] Figure 2 A cross-sectional schematic diagram of the magnetic adsorption device in Example 1 of the present invention;

[0036] Figure 3 A cross-sectional schematic diagram of a magnetic rod fixing assembly in Example 1 of the present invention;

[0037] Figure 4 A cross-sectional schematic diagram of a magnetic bar fixing assembly in Example 2 of the present invention;

[0038] Figure 5 A cross-sectional schematic diagram of a magnetic conductive rod in Example 5 of the present invention;

[0039] Figure 6 A cross-sectional schematic diagram of a controllable magnetic rod in Example 6 of the present invention;

[0040] Figure 7 A schematic diagram of a magnetic bead purification instrument in Example 7 of the present invention;

[0041] Figure 8 A schematic diagram of a magnetic bead purification instrument in Example 8 of the present invention;

[0042] In the figure: a base 100, a lifting device 200, a translation device 300, a magnetic adsorption device 400, a magnetic rod fixing assembly 410, a magnetic rod cavity 411, a fixing groove 412, an oscillation device 413, a controllable magnetic rod 420, a magnetic rod core 421, a magnetic rod coil 422, a magnetic rod 430, a magnetic rod shell 431, a control device 500, a heating device 600, a shell 700, a window 710, a human-computer interaction device 800, and a reagent kit 900. DETAILED DESCRIPTION

[0043] It should be noted that, in the absence of conflict, the embodiments of the present invention and the features in the embodiments can be combined with each other. The present invention will be described in detail below with reference to the accompanying drawings and in combination with the embodiments.

[0044] In order to enable those skilled in the art to better understand the scheme of the present invention, the technical scheme in the embodiment of the present invention will be clearly and completely described below in conjunction with the drawings in the embodiment of the present invention. Obviously, the described embodiment is only a part of the embodiment of the present invention, not all of the embodiments. Based on the embodiment of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0045] In the present invention, unless otherwise specified, all equipment and raw materials can be purchased from the market or are commonly used in the industry. The methods in the following embodiments, unless otherwise specified, are conventional methods in the art.

[0046] As introduced in the background technology, the magnetic bead purifier uses a permanent magnet to adsorb the magnetic beads, which makes desorption difficult. It is necessary to add structures such as a magnetic rod sleeve to solve this problem, but this increases the structural complexity of the magnetic bead purifier.

[0047] In order to solve this problem, the applicant of the present invention provides a magnetic bead purification instrument.

[0048] like Figure 1 As shown, it includes a base 100, a lifting device 200, a translation device 300, a magnetic adsorption device 400 and a control device 500;

[0049] The lifting device 200 is arranged on the base 100, and is used to realize movement in the vertical direction; the translation device 300 is arranged on the lifting device 200, and is used to realize movement in the horizontal direction; the magnetic adsorption device 400 is arranged in connection with the translation device 300, and is used to adsorb and disperse magnetic beads; the control device 500 is arranged on the base 100, is electrically connected to the lifting device 200 and controls the lifting device 200 to realize movement in the vertical direction, is electrically connected to the translation device 300 and controls the translation device 300 to realize movement in the horizontal direction, and is electrically connected to the controllable magnetic rod 420 on the magnetic adsorption device 400 and controls the presence or absence of magnetism of the controllable magnetic rod 420;

[0050] Further, such as Figure 2 As shown, the magnetic adsorption device 400 includes a magnetic bar fixing component 410, a controllable magnetic bar 420 and a plurality of magnetic rods 430; one end of the magnetic bar fixing component 410 is connected to the translation device 300, as shown in FIG. Figure 3 As shown, it also includes a magnetic rod cavity 411 and a fixing groove 412, the magnetic rod cavity 411 is used to accommodate the controllable magnetic rod 420, and the fixing groove 412 is used to fix the magnetic rod 430; the controllable magnetic rod 420 is arranged in the magnetic rod cavity 411 of the magnetic rod fixing assembly 410, and the presence or absence of magnetism can be controlled by electric current; the magnetic rod 430 is arranged in the fixing groove 412 and is perpendicular to the controllable magnetic rod 420, and the presence or absence of magnetism is realized under the control of the magnetic field generated by the controllable magnetic rod 420, and is used to contact the sample and adsorb and disperse the magnetic beads in the sample.

[0051] In the existing magnetic bead purifier, a permanent magnet is used as a magnetic rod to adsorb and separate the magnetic beads in the sample to be processed. Since the permanent magnet cannot control the presence or absence of magnetism and is always in a magnetic state, a magnetic rod cover and a corresponding control mechanism need to be provided to allow the magnetic rod cover to isolate the magnetic beads from the magnetic rod during the magnetic bead adsorption process. When separation is required, the magnetic rod is taken out of the magnetic rod cover, so that the magnetic beads on the surface of the magnetic rod cover lose the force of attraction, thereby achieving the separation of the magnetic beads; however, the additional set of magnetic rod covers and corresponding control systems undoubtedly increase the complexity of the magnetic bead purifier, and also become an obstacle to the further miniaturization and portability of the magnetic bead purifier. The present invention proposes to use an electromagnet instead of a permanent magnet, control the presence or absence of magnetism of the electromagnet by electric current, directly achieve magnetic adsorption and separation, eliminate the process of isolating the magnetic rod and the magnetic beads by the magnetic rod cover, and can also eliminate a series of mechanical structures including the magnetic rod cover and the magnetic rod cover control structure, simplify the magnetic bead purifier, and facilitate the further development of the magnetic bead purifier in the direction of miniaturization and portability.

[0052] Furthermore, at least two groups of magnetic adsorption devices 400 are included, and the magnetic adsorption devices 400 include a magnetic rod fixing component 410, a controllable magnetic rod 420 and at least two magnetic rods 430; the control device 500 controls all the magnetic adsorption devices to move together, and can also independently control a group of magnetic adsorption devices or several groups of magnetic adsorption devices to move; for the control of the magnetic properties of the controllable magnetic rods in different magnetic adsorption devices, the same control method or a separate control method can be adopted according to actual conditions; and the different magnetic rods in one magnetic adsorption device are controlled by a controllable magnetic rod, so the magnetic rods in the same magnetic adsorption device have magnetism or lose magnetism at the same time within the allowable error range.

[0053] Different magnetic adsorption devices are independent of each other, and the control device can control them individually or in a unified manner. For example, when there are many samples to be tested and the magnetic bead purifier is operating at full capacity, all magnetic adsorption devices can be controlled in a unified manner, which can improve the efficiency of the entire separation and purification process. For a small number of samples and not operating at full capacity, only some of the magnetic adsorption devices that need to be operated can be controlled, which can reduce operating costs. The magnetic rods on a magnetic adsorption device are controlled by the same controllable magnetic rod, so they have magnetism or lose magnetism at the same time within the allowable error range.

[0054] Further, such as Figure 4 As shown, an oscillating device 413 is provided in the magnetic bar fixing device, and the magnetic rod 430 vibrates under the action of the oscillating device 413 . Meanwhile, the oscillating device 413 is electrically connected to the control device 500 , and the control device 500 controls the movement of the oscillating device 413 .

[0055] The magnetism of the controllable magnetic rod can be controlled by electric current, and the magnetic rod has magnetism. However, in daily use, when the controllable magnetic rod has lost its magnetism, the magnetic rod will still have a certain magnetism for a short period of time. In addition, even if the magnetic rod completely loses its magnetism, the magnetic beads will still adhere to the side wall of the magnetic rod due to the lag in the disappearance of magnetism or surface adsorption. An oscillation device is provided to enable the magnetic beads to detach from the controllable magnetic rod as soon as possible, thereby realizing the separation process of the magnetic beads. The oscillation device is controlled by the control device, and the vibration device is started after the controllable magnetic rod loses its magnetism to assist in the separation of the magnetic beads.

[0056] Furthermore, the oscillation device 413 is an ultrasonic oscillation device.

[0057] The ultrasonic oscillation device generates ultrasonic waves and acts on the magnetic rod fixing assembly, eventually causing the magnetic rod to vibrate, so that the magnetic beads still attached to the magnetic rod are detached from the magnetic rod and enter the liquid under the action of vibration, thereby achieving complete separation of the magnetic beads.

[0058] Further, such as Figure 5 As shown, the magnetic rod 430 is provided with a magnetic rod housing 431 outside. The magnetic rod housing 431 is an integrally formed conical tube, and the bottom end of the magnetic rod housing 431 is a curved surface.

[0059] The magnetic rod is made of soft iron or silicon steel, which is prone to rust when in contact with the liquid in the sample. Therefore, a shell is set on the outside of the magnetic rod to isolate the sample liquid from the magnetic rod. At the same time, the one-piece molding can have better isolation ability to prevent cracks and other problems that may affect the magnetic rod after long-term use. The lower end of the magnetic rod shell is a curved surface, which is conducive to the magnetic rod to penetrate into the test tube or reagent box containing the sample.

[0060] Furthermore, the magnetic rod housing 431 is a coating formed on the outer side of the magnetic rod by coating.

[0061] The magnetic rod shell can be a pre-integrated high molecular polymer shell. The magnetic rod shell can isolate the magnetic rod and the sample to prevent the magnetic rod from contaminating the sample. It can also protect the magnetic rod from being corroded by the sample or certain components in the sample, thereby ensuring the service life of the magnetic rod. It can also be a coating formed by applying a corresponding coating to the outside of the magnetic rod. The coated shell has the characteristics of being easier to manufacture and maintain, and can be repaired in time according to the situation.

[0062] Further, such as Figure 6 As shown, the controllable magnetic rod 420 includes a magnetic rod core 421 and a magnetic rod coil 422. The magnetic rod core 421 is made of soft iron or silicon steel and is used to enhance the magnetism of the controllable magnetic rod 420. The magnetic rod coil 422 is spirally wound on the surface of the magnetic rod core 421 and is electrically connected to the control device 500, giving the controllable magnetic rod 420 magnetism.

[0063] Using soft iron or silicon steel as the magnetic rod core can make the controllable magnetic rod demagnetized immediately after power failure, avoiding the problem that the controllable magnetic rod cannot be demagnetized quickly after power failure. The combination of magnetic rod core and magnetic rod coil can make the generated magnetism stronger, more stable and controllable.

[0064] Further, such as Figure 7 As shown, the base is also provided with a heating device 600 for heating and keeping the sample warm when necessary.

[0065] A heating device is provided to heat certain special samples that need to be heated or kept warm. At the same time, when used in a low temperature environment, the samples can also be heated to keep them in a suitable state or temperature.

[0066] Further, such as Figure 8As shown, the magnetic bead purifier also includes a shell 700 for accommodating various parts of the magnetic bead purifier, including but not limited to the lifting device 200, the translation device 300, the magnetic adsorption device 400 and the control device 500; and also includes a human-computer interaction device 800, which is electrically connected to the control device 500 and is used for the operator to control and monitor the operation of the magnetic bead purifier.

[0067] The shell is used to protect the components of the magnetic bead purifier from external interference and pollution, including interference from dust, accidental touch by personnel and electromagnetic interference under special circumstances; the human-computer interaction device can be a display screen with a touch screen control function and a function of monitoring the operation of the magnetic bead purifier, or it can be a combination of a display screen and physical buttons, so that the operator can control the magnetic bead purifier in a simple and easy-to-learn way and obtain the operating status information of the magnetic bead purifier.

[0068] Furthermore, a window 710 is provided on the side of the housing 700 for installing and unloading the reagent kit 900 for carrying the sample and reagents, or allowing the magnetic adsorption device 400 to extend out of the housing 700 .

[0069] A window is arranged on the side of the shell for the sample to enter and exit the magnetic bead purifier, and also allows the magnetic adsorption device in the magnetic bead purifier to extend out of the shell of the magnetic bead purifier, so as to facilitate the processing of some special samples.

[0070] The technical solution of the present invention will be further described below in conjunction with embodiments.

[0071] Example 1

[0072] like Figure 1 As shown, a magnetic bead purification instrument includes a base 100, a lifting device 200, a translation device 300, a magnetic adsorption device 400 and a control device 500;

[0073] The lifting device 200 is arranged on the base 100, the translation device 300 is arranged on the lifting device 200, and the magnetic adsorption device 400 is arranged and connected to the translation device 300; the control device 500 is arranged on the base 100, is electrically connected to the lifting device 200 and controls the lifting device 200 to realize vertical movement, is electrically connected to the translation device 300 and controls the translation device 300 to realize horizontal movement, and is electrically connected to the controllable magnetic rod 420 on the magnetic adsorption device 400 and controls the presence or absence of magnetism of the controllable magnetic rod 420;

[0074] like Figure 2As shown, the magnetic adsorption device 400 includes a magnetic bar fixing component 410, a controllable magnetic bar 420 and a plurality of magnetic rods 430; one end of the magnetic bar fixing component 410 is connected to the translation device 300, as shown in FIG. Figure 3 As shown, it also includes a magnetic rod cavity 411 and a fixing groove 412, the magnetic rod cavity 411 is used to accommodate the controllable magnetic rod 420, and the fixing groove 412 is used to fix the magnetic rod 430; the controllable magnetic rod 420 is arranged in the magnetic rod cavity 411 of the magnetic rod fixing assembly 410, and the presence or absence of magnetism can be controlled by electric current; the magnetic rod 430 is arranged in the fixing groove 412 and is perpendicular to the controllable magnetic rod 420, and the presence or absence of magnetism is realized under the control of the magnetic field generated by the controllable magnetic rod 420, and is used to contact the sample and adsorb and disperse the magnetic beads in the sample.

[0075] Example 2

[0076] Except for the following parts, other parts in this embodiment are the same as those in Embodiment 1.

[0077] like Figure 4 As shown, it also includes that an oscillation device 413 is provided in the magnetic bar fixing device, and the magnetic rod 430 vibrates under the action of the oscillation device 413. At the same time, the oscillation device 413 is electrically connected to the control device 500, and the control device 500 controls the movement of the oscillation device 413; the oscillation device 413 is a mechanical oscillation device.

[0078] Example 3

[0079] Except for the following parts, other parts in this embodiment are the same as those in Embodiment 2.

[0080] The oscillation device 413 is an ultrasonic oscillation device.

[0081] Example 4

[0082] Except for the following parts, other parts in this embodiment are the same as those in Embodiment 1.

[0083] like Figure 5 As shown, the magnetic rod 430 is provided with a magnetic rod housing 431 outside. The magnetic rod housing 431 is an integrally formed conical tube, and the bottom end of the magnetic rod housing 431 is a curved surface.

[0084] Example 5

[0085] Except for the following parts, other parts in this embodiment are the same as those in Embodiment 4.

[0086] The magnetic rod housing 431 is a coating formed on the outer side of the magnetic rod by coating.

[0087] Example 6

[0088] Except for the following parts, other parts in this embodiment are the same as those in Embodiment 1.

[0089] like Figure 6 As shown, the controllable magnetic rod 420 includes a magnetic rod core 421 and a magnetic rod coil 422. The magnetic rod core 421 is made of soft iron or silicon steel and is used to enhance the magnetism of the controllable magnetic rod 420. The magnetic rod coil 422 is spirally wound on the surface of the magnetic rod core 421 and is electrically connected to the control device 500, giving the controllable magnetic rod 420 magnetism.

[0090] Example 7

[0091] Except for the following parts, other parts in this embodiment are the same as those in Embodiment 1.

[0092] like Figure 7 As shown, the base is also provided with a heating device 600 for heating and keeping the sample warm when necessary.

[0093] Example 8

[0094] Except for the following parts, other parts in this embodiment are the same as those in Embodiment 1.

[0095] like Figure 8 As shown, the magnetic bead purifier also includes a shell 700 for accommodating various parts of the magnetic bead purifier, including but not limited to the lifting device 200, the translation device 300, the magnetic adsorption device 400 and the control device 500; it also includes a human-computer interaction device 800, which is electrically connected to the control device 500 and is used for operators to control and monitor the operation of the magnetic bead purifier; a window 710 is provided on the side of the shell 700 for installing and unloading a test kit 900 for carrying the samples and reagents.

[0096] Example 9

[0097] like Figure 1 As shown, a magnetic bead purification instrument includes a base 100, a lifting device 200, a translation device 300, a magnetic adsorption device 400 and a control device 500;

[0098] The lifting device 200 is arranged on the base 100, and is used to realize movement in the vertical direction; the translation device 300 is arranged on the lifting device 200, and is used to realize movement in the horizontal direction; the magnetic adsorption device 400 is arranged in connection with the translation device 300, and is used to adsorb and disperse magnetic beads; the control device 500 is arranged on the base 100, is electrically connected to the lifting device 200 and controls the lifting device 200 to realize movement in the vertical direction, is electrically connected to the translation device 300 and controls the translation device 300 to realize movement in the horizontal direction, and is electrically connected to the controllable magnetic rod 420 on the magnetic adsorption device 400 and controls the presence or absence of magnetism of the controllable magnetic rod 420;

[0099] like Figure 2 As shown, the magnetic adsorption device 400 includes a magnetic bar fixing component 410, a controllable magnetic bar 420 and a plurality of magnetic rods 430; one end of the magnetic bar fixing component 410 is connected to the translation device 300, as shown in FIG. Figure 3 As shown, it also includes a magnetic rod cavity 411 and a fixing groove 412, the magnetic rod cavity 411 is used to accommodate the controllable magnetic rod 420, and the fixing groove 412 is used to fix the magnetic rod 430; the controllable magnetic rod 420 is arranged in the magnetic rod cavity 411 of the magnetic rod fixing assembly 410, and the presence or absence of magnetism can be controlled by electric current; the magnetic rod 430 is arranged in the fixing groove 412 and is perpendicular to the controllable magnetic rod 420, and the presence or absence of magnetism is realized under the control of the magnetic field generated by the controllable magnetic rod 420, and is used to contact with the sample and adsorb and disperse the magnetic beads in the sample; as shown Figure 4 As shown, the magnetic rod fixing device is provided with an oscillating device 413, and the magnetic rod 430 vibrates under the action of the oscillating device 413. At the same time, the oscillating device 413 is electrically connected to the control device 500, and the control device 500 controls the movement of the oscillating device 413. The oscillating device 413 is an ultrasonic oscillating device; Figure 5 As shown, the magnetic rod 430 is provided with a magnetic rod housing 431, and the magnetic rod housing 431 is a coating formed by coating on the outside of the magnetic rod, and the bottom end of the magnetic rod housing 431 is a curved surface; Figure 6 As shown, the controllable magnetic bar 420 includes a magnetic bar core 421 and a magnetic bar coil 422. The magnetic bar core 421 is made of soft iron or silicon steel and is used to enhance the magnetism of the controllable magnetic bar 420. The magnetic bar coil 422 is spirally wound on the surface of the magnetic bar core 421 and is electrically connected to the control device 500 to give the controllable magnetic bar 420 magnetism.

[0100] like Figure 8As shown, the magnetic bead purifier also includes a shell 700, which is used to accommodate various parts of the magnetic bead purifier, including but not limited to the lifting device 200, the translation device 300, the magnetic adsorption device 400 and the control device 500; it also includes a human-computer interaction device 800, which is electrically connected to the control device 500 and is used for operators to control and monitor the operation of the magnetic bead purifier; a window 710 is provided on the side of the shell 700 for installing and unloading the test kit 900 for carrying the samples and reagents, or allowing the magnetic adsorption device 400 to extend out of the shell 700.

[0101] It should be understood that those skilled in the art can make improvements or changes based on the above description, and all these improvements and changes should fall within the scope of protection of the appended claims of the present invention.

Claims

1. A magnetic bead purification instrument, used to remove impurities in a sample by an immunomagnetic bead method to improve the purity of the sample, characterized in that: include: A base (100) used to carry and fix the components on the magnetic bead purification instrument; A lifting device (200), disposed on the base (100) and used to achieve movement in a vertical direction; A translation device (300), arranged on the lifting device (200), and used to achieve movement in a horizontal direction; The magnetic adsorption device (400) is used for adsorbing and dispersing magnetic beads, comprising: A magnetic bar fixing assembly (410), one end of which is connected to the translation device (300), comprises: The magnetic bar cavity (411) is arranged in the middle of the magnetic bar fixing assembly (410). A fixing groove (412) is provided at the bottom of the magnetic bar fixing assembly (410); An oscillating device (413) is disposed in the fixing groove (412); A controllable magnetic rod (420) is disposed in the magnetic rod cavity (411) of the magnetic rod fixing assembly (410), and the presence or absence of magnetism can be controlled by electric current; a control device (500) disposed on the base (100), electrically connected to the lifting device (200) and controlling the lifting device (200) to achieve vertical movement, electrically connected to the translation device (300) and controlling the translation device (300) to achieve horizontal movement, and electrically connected to the controllable magnetic rod (420) and controlling the presence or absence of magnetism of the controllable magnetic rod; A plurality of magnetic rods (430) are arranged in the fixing groove (412) and are perpendicular to the controllable magnetic rod (420). The magnetic field generated by the controllable magnetic rod (420) controls the presence or absence of magnetism and is used to contact the sample and adsorb and disperse the magnetic beads in the sample. The magnetic rods (430) vibrate under the action of the oscillating device (413). A magnetic rod housing (431) is provided outside the magnetic rod (430), and the magnetic rod housing (431) is a The controllable magnetic rod (420) comprises a magnetic rod core (421) and a magnetic rod coil (422). The magnetic rod core (421) is made of soft iron or silicon steel and is used to enhance the magnetic properties of the controllable magnetic rod (420). The magnetic rod coil (422) is spirally wound on the surface of the magnetic rod core (421) and is electrically connected to the control device (500) to impart magnetic properties to the controllable magnetic rod (420).

2. A magnetic bead purification instrument according to claim 1, characterized in that: The oscillating device (413) is electrically connected to the control device (500), and the control device (500) controls the movement of the oscillating device (413).

3. A magnetic bead purification instrument according to claim 1, characterized in that: The oscillation device (413) is an ultrasonic oscillation device.

4. A magnetic bead purification instrument according to claim 1, characterized in that: The magnetic rod housing (431) is a coating formed on the outside of the magnetic rod by coating.

5. A magnetic bead purification instrument according to claim 1, characterized in that, Also includes: The heating device (600) is disposed on the base (100), is electrically connected to the control device (500), and is used to perform a heating process on the sample.

6. A magnetic bead purification instrument according to claim 1, characterized in that: Also includes: A housing (700) is fixed on the base (100) and forms a cavity, and is used to accommodate the lifting device (200), the translation device (300), the magnetic adsorption device (400), and the control device (500); The human-machine interaction device (800) is electrically connected to the control device (500) and is used by an operator to control and monitor the operation of the magnetic bead purification instrument.

7. A magnetic bead purification instrument according to claim 6, characterized in that: A window (710) is provided on the side of the housing (700) for installing and unloading a reagent kit (900) for carrying the sample and reagents.

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