Organoid culture equipment

Abstract

The present invention discloses an organoid culture device, comprising: a platform component, which includes a base platform and a culture dish disposed on the base platform for placing organoids; a lid-lifting component, which includes a rotating lid, a locking mechanism, and an operation window; and a lid-moving component, which includes a pressing lid mechanism connected to the rotating lid and a translation lid slidably disposed along the X-axis between the rotating lid and the pressing lid mechanism, and the translation lid is driven to reciprocally slide along the X-axis to switch between an open-lid state and a closed-lid state. The organoid culture chamber of the present invention adopts two lid-opening methods, namely a lid-lifting type and a lid-pushing type. The former is convenient for placing the culture dish and cleaning the chamber, and the latter can facilitate automated lid-opening and lid-closing operations during drug administration to the internal organoids; the present invention can provide a stable temperature, humidity, and gas environment for the organoids, so that the temperature and humidity inside the culture chamber are maintained within the optimal temperature range for organoid development.

Description

Technical Field

[0001] The present invention relates to the technical field of organoid culture, and particularly relates to an organoid culture device. Background Art

[0002] With the development of science and technology, organoid culture technology has enabled cells to differentiate into cell types specific to organoids. Through organoid culture technology, cell cultures can have a similar spatial organization to the organs they represent and some functions of the corresponding organs, thereby being able to highly simulate the development of human organs in vitro and reconstruct the structure of human organs to replace animal models, providing more possibilities for the study of human diseases.

[0003] Currently, most organoid cultures need to be carried out in a specific incubator, which can provide the required development conditions for organoid culture. However, currently, when it is necessary to observe the development situation and operate on the culture medium during the organoid culture process, the organoids need to be taken out of the incubator, which will affect the development of the organoids every time of observation and operation, and thus also affect the experimental results.

[0004] In order to study the effects of different drugs on the development of organoids, it is necessary to administer drugs to the organoids in the culture chamber during the culture process. With the development of current automation technology and level, the administration of drugs to organoids can be carried out automatically. The prerequisite for automatic drug administration is to first open the lid of the culture chamber to expose the organoids. The traditional way of opening the lid by lifting is beneficial for operating the culture dish and internal sterilization before culture, but it is not convenient for automatic lid opening operation. Summary of the Invention

[0005] The technical problem to be solved by the present invention is to provide an organoid culture device aiming at the deficiencies in the above-mentioned prior art.

[0006] To solve the above technical problem, the technical solution adopted by the present invention is: an organoid culture device, comprising:

[0007] A platform assembly, which includes a base platform and a culture dish provided on the base platform for placing organoids;

[0008] A lid-lifting assembly, which includes a rotating lid rotatably connected to the base platform around the Y-axis, a locking mechanism provided on the rotating lid for locking the rotating lid on the base platform, and an operation window opened in the middle of the rotating lid. When the rotating lid is buckled on the base platform, the operation window is located above the culture dish;

[0009] and a cover moving component, which includes a gland pressing mechanism connected to the rotating cover and a translation cover slidably arranged along the X-axis between the rotating cover and the gland pressing mechanism. The translation cover is driven to reciprocally slide along the X-axis to switch between an open cover state and a closed cover state. In the open cover state, the translation cover leaves above the operation window, and in the closed cover state, the translation cover completely covers the operation window.

[0010] Preferably, the gland pressing mechanism includes two gland pressing strips with an L-shaped cross-section symmetrically connected to the rotating cover about the X-axis, and a plurality of gland pressing ball plungers arranged at intervals on the gland pressing strips along the X-direction;

[0011] A gap between the gland pressing strip and the rotating cover forms a translation chute for the two sides of the translation cover to be inserted and fitted. The ball at the bottom of the gland pressing ball plunger extends downward into the translation chute.

[0012] Preferably, translation rails are formed on both sides of the translation cover along the X-direction. Both ends of the translation rails along the X-direction protrude from the translation cover. A V-shaped groove for the ball of the gland pressing ball plunger to be fitted and pressed into is formed on the surface of the translation rails. A guide surface is recessed upward on the bottom surface of one end of the translation rails close to the locking mechanism. The guide surface and the bottom surface of the translation rails are transitioned through an arc slope.

[0013] Preferably, a top cover component for cooperating with the translation rails is arranged on the inner side of the gland pressing strip. The top cover component includes a top cover shaft connected to the gland pressing strip and a bearing rotatably connected to the top cover shaft. The bearing is used to generate an upward pressing force on the bottom surface of the translation rails when the translation cover reciprocally slides along the X-axis.

[0014] Preferably, a push cover hole is formed on the translation cover, and the translation cover is slid by applying a thrust in the X-axis direction to the translation cover;

[0015] A plurality of lighting holes are formed on the translation cover, and double-layer glass is arranged on the lighting holes.

[0016] Preferably, the locking mechanism includes two locking seats connected to the rotating cover, a rotating shaft rotatably connected to the locking seats, a handle connected to the rotating shaft, a limiting block arranged on the side of the handle, a locking hook arranged at the bottom of the handle, and a torsion spring sleeved on the rotating shaft and located between the limiting block and the locking seat.

[0017] Preferably, this kind of organ culture device further includes a first upper temperature control component arranged on the translation cover and a second upper temperature control component arranged on the rotating cover. The first upper temperature control component includes a first upper heating belt, a first upper temperature sensor, and a first over-temperature sensor arranged on the translation cover;

[0018] The second upper temperature control component includes a second upper heating belt, a second upper temperature sensor, and a second over-temperature sensor disposed on the rotary cover.

[0019] Preferably, the organ culture device further includes a communication connection component, which includes a communication module disposed on the rotary cover, a lower circuit board connected to the communication module, an upper circuit board connected to the translation cover, and a plurality of elastic pieces disposed on the upper circuit board. The first upper temperature sensor and the first over-temperature sensor are both communicatively connected to the upper circuit board. When the translation cover is closed, the elastic pieces contact and conduct with corresponding contacts on the lower circuit board;

[0020] A conductive ball plunger is further connected to the translation cover. The first upper heating belt is electrically connected to the conductive ball plunger. When the translation cover is closed, the lower end of the conductive ball plunger contacts and conducts with a corresponding contact on the lower circuit board.

[0021] Preferably, the organ culture device further includes a lower temperature control component disposed on the base platform. The lower temperature control component includes a lower heating belt, a lower temperature sensor, and a lower over-temperature sensor disposed on the base platform.

[0022] Preferably, the organ culture device further includes a humidity control component, which includes a humidifying bottle disposed on the base platform, a humidifying container communicated with the humidifying bottle through a humidifying pipeline, an air inlet needle with an air outlet extending into the interior of the humidifying container, and a humidity sensor disposed above the humidifying container.

[0023] Preferably, the organ culture device further includes a liquid path heating member disposed on the base platform. The liquid path heating member includes a plurality of liquid inlet pipelines horizontally disposed on the base platform and a heating plate covering the liquid inlet pipelines. A pressure pipe groove matching the liquid inlet pipelines is formed on the bottom surface of the heating plate.

[0024] Preferably, a plurality of observation holes are formed in the base platform at a position below the culture dish, and glass sheets are disposed on the observation holes;

[0025] A hinge for connecting with the rotary cover is disposed at the end of the base platform, and a barb for cooperating with the locking hook is disposed at the other end of the base platform opposite to the hinge.

[0026] Preferably, the organ culture device further includes an upper switch cover monitoring component and a lower switch cover monitoring component. The upper switch cover monitoring component includes an upper groove-shaped optocoupler disposed on the rotary cover and an upper optocoupler positioning pin disposed on the translation cover for cooperating with the upper groove-shaped optocoupler;

[0027] The lower switch cover monitoring component includes a lower groove optical coupler disposed on the base platform and a lower optical coupler shutter disposed on the rotating cover for cooperating with the lower groove optical coupler.

[0028] Preferably, an annular groove is formed on the bottom surface of the rotating cover, and a sealing ring is disposed in the annular groove.

[0029] The beneficial effects of the present invention are as follows:

[0030] The organoid culture chamber of the present invention adopts two opening methods, namely, the flip-up type and the push-pull type. The former is convenient for placing the culture dish and cleaning the chamber, and the latter can facilitate the automatic opening and closing of the cover during the drug administration operation of the internal organoids.

[0031] The present invention can provide a stable temperature, humidity and gas environment for the organoids, so that the temperature and humidity inside the culture chamber are maintained within the optimal temperature range for organoid development.

[0032] The organoid culture chamber of the present invention is provided with lighting and observation holes, which is convenient for observing the development of the organoids in real time during the culture process.

[0033] The communication connection component in the push cover assembly of the present invention can maintain the communication between the sensor and the heating belt and the outside when the moving cover is closed, and disconnect the communication when the moving cover is opened, avoiding the influence of the wire harness connection on the moving process and the space occupation. Description of the Drawings

[0034] Figure 1 It is a schematic structural view of the organoid culture device of the present invention;

[0035] Figure 2 It is a schematic structural view of the organoid culture device of the present invention after removing the translation cover;

[0036] Figure 3 It is a schematic structural view of the cooperation between the flip-up cover assembly and the moving cover assembly of the present invention;

[0037] Figure 4 It is a schematic structural view of the bottom of the flip-up cover assembly of the present invention;

[0038] Figure 5 It is a schematic structural view of the moving cover assembly of the present invention;

[0039] Figure 6 It is a schematic structural view of the cooperation between the translation cover and the pressing cover mechanism of the present invention;

[0040] Figure 7 It is a schematic structural view of the translation cover of the present invention;

[0041] Figure 8Schematic cross-sectional structure diagram of the translation cover and the gland mechanism of the present invention;

[0042] Figures 9 - 10 Schematic process diagram of automatic drug addition by the organoid culture device of the present invention;

[0043] Figure 11 Schematic structure diagram of the communication connection component of the present invention;

[0044] Figure 12 Schematic structure diagram of the top cover component of the present invention;

[0045] Figure 13 Schematic structure diagram of the locking mechanism of the present invention;

[0046] Figure 14 Schematic structure diagram of the platform component of the present invention;

[0047] Figure 15 Schematic structure diagram of the heating plate of the present invention;

[0048] Figure 16 Schematic structure diagram of the base platform of the present invention.

[0049] Explanation of reference numerals:

[0050] 1 - Platform component; 10 - Base platform; 11 - Petri dish; 12 - Observation hole; 13 - Hinge; 14 - Barb;

[0051] 2 - Lid-lifting component; 20 - Rotating cover; 21 - Locking mechanism; 22 - Operation window; 200 - Annular groove; 210 - Locking seat; 211 - Rotating shaft; 212 - Handle; 213 - Limiting block; 214 - Lock hook; 215 - Torsion spring;

[0052] 3 - Cover-moving component; 30 - Gland mechanism; 31 - Translation cover; 32 - Gland strip; 33 - Gland ball plunger; 34 - Translation chute; 35 - Translation slide rail; 36 - Top cover component; 37 - Push rod; 310 - Push cover hole; 311 - Lighting hole; 312 - Double-layer glass; 350 - V-shaped groove; 351 - Guide surface; 352 - Arc ramp; 360 - Top cover shaft; 361 - Bearing; 362 - Snap ring;

[0053] 40 - First upper temperature control component; 41 - Second upper temperature control component; 42 - Lower temperature control component; 43 - First upper temperature sensor, first over-temperature sensor; 44 - Second upper heating tape; 45 - Second upper temperature sensor, second over-temperature sensor; 46 - Lower heating tape; 47 - Lower temperature sensor, lower over-temperature sensor;

[0054] 5 - Communication connection component; 50 - Lower circuit board; 51 - Upper circuit board; 52 - Spring piece; 53 - Conductive ball plunger;

[0055] 6 - Humidity control component; 60 - Humidification bottle; 61 - Humidification container; 62 - Intake needle; 63 - Humidity sensor;

[0056] 7 - Liquid path heating element; 70 - Liquid inlet pipeline; 71 - Heating plate; 72 - Pipe pressing groove;

[0057] 8 - Upper switch cover monitoring component; 80 - Upper groove type optocoupler; 81 - Upper optocoupler baffle;

[0058] 9 - Lower switch cover monitoring component; 90 - Lower groove type optocoupler. Detailed implementation mode

[0059] The following further elaborates on the present invention in conjunction with embodiments, so that those skilled in the art can implement it with reference to the text of the specification.

[0060] It should be understood that terms such as "having", "comprising", and "including" used herein do not exclude the presence or addition of one or more other elements or their combinations.

[0061] Embodiment 1

[0062] As Figures 1 - 16 shown, an organoid culture device of this embodiment includes:

[0063] Platform component 1, which includes a base platform 10 and a culture dish 11 disposed on the base platform 10 for placing organoids;

[0064] Lid - lifting component 2, which includes a rotating lid 20 rotatably connected to the base platform 10 around the Y - axis, a locking mechanism 21 disposed on the rotating lid 20 for locking the rotating lid 20 on the base platform 10, and an operation window 22 opened in the middle of the rotating lid 20. When the rotating lid 20 is buckled on the base platform 10, the operation window 22 is above the culture dish 11;

[0065] And a lid - moving component 3, which includes a pressing - lid mechanism 30 connected to the rotating lid 20 and a translation lid 31 slidably disposed between the rotating lid 20 and the pressing - lid mechanism 30 along the X - axis. The translation lid 31 is driven to reciprocate along the X - axis to switch between the open - lid state and the closed - lid state. In the open - lid state, the translation lid 31 moves away from above the operation window 22, and in the closed - lid state, the translation lid 31 completely covers the operation window 22.

[0066] The organoid culture device of the present invention provides two lid-opening functions: a flip-open method and a translation-open method. When manual operation inside the culture chamber is required, the flip-open method is adopted. The opening area is large, which is convenient for operation, but the impact on the internal environment is relatively greater. When performing operations such as adding drugs to the organoids in the culture chamber during the organoid culture process, the translation-open method is adopted. It does not occupy the space in the Z-axis direction, which is convenient for the layout and movement operation of automated equipment, and has less impact on the internal environment. The following further describes the present invention.

[0067] In this embodiment, the gland pressing mechanism 30 includes two gland pressing strips 32 with an L-shaped cross-section symmetrically connected to the rotating cover 20 about the X-axis, and a plurality of gland pressing ball plungers 33 arranged at intervals along the X-direction on the gland pressing strips 32; a translation chute 34 for the two sides of the translation cover 31 to be inserted into is formed by the gap between the gland pressing strips 32 and the rotating cover 20, and the ball head at the bottom of the gland pressing ball plunger 33 extends downward into the translation chute 34.

[0068] An annular groove 200 is formed on the bottom surface of the rotating cover 20, and a sealing ring is arranged in the annular groove 200. Correspondingly, a groove is also formed around the periphery of the operation window 22, and there is also a sealing ring inside.

[0069] The gland pressing ball plunger 33 can provide a downward pressure for the translation cover 31 to ensure that the translation cover 31 can be tightly pressed against the rotating cover 20 by the sealing element in the closed lid state, so as to ensure the airtightness inside the culture chamber in the closed lid state.

[0070] Among them, translation rails 35 are formed on both sides of the translation cover 31 along the X-direction. The two ends of the translation rails 35 along the X-direction protrude from the translation cover 31. A V-shaped groove 350 for the ball head of the gland pressing ball plunger 33 to be inserted into is formed on the surface of the translation rails 35. A guiding surface 351 is formed by the bottom surface of the translation rails 35 near one end of the locking mechanism 21 being recessed upward, and the guiding surface 351 and the bottom surface of the translation rails 35 are transitioned by an arc slope 352.

[0071] Among them, a top cover assembly 36 for cooperating with the translation rails 35 is arranged on the inner side of the gland pressing strips 32. The top cover assembly 36 includes a top cover shaft 360 connected to the gland pressing strips 32, a bearing 361 rotatably connected to the top cover shaft 360, and a snap ring 362 arranged at the end. The bearing 361 is used to generate an upward pressing force on the bottom surface of the translation rails 35 when the translation cover 31 reciprocates along the X-axis.

[0072] A push cover hole 310 is formed on the translation cover 31, and a thrust in the X-axis direction is applied to the translation cover 31 through a push rod 37 to realize the sliding of the translation cover 31.

[0073] When the translation cover 31 slides open under an external force, the external push rod 37 is inserted into the push cover hole 310 to drive the translation cover 31 to move. The bearing 361 of the top cover assembly 36 contacts the guiding surface 351 at the bottom of the translation slide rail 35, and then contacts the bottom surface (higher than the guiding surface 351) of the translation slide rail 35 after passing through the arc ramp 352, so that the translation cover 31 is lifted upward, ensuring that there is a gap between the translation cover 31 and the rotating cover 20, so that there is no sliding friction resistance during the pushing process, and ensuring that the translation cover 31 can be pushed open and closed smoothly. When the translation cover 31 is opened, the external push rod 37 is removed, and the drug delivery mechanism moves to the position of the hole of the culture dish 11 where drug delivery is required, and the operation of automatically delivering drugs to the organoids in the culture dish 11 can be realized (refer to Figures 9 - 10 ).

[0074] In a preferred embodiment, a plurality of lighting holes 311 are formed in the translation cover 31, and a double-layer glass 312 is arranged on the lighting holes 311 for lighting when observing the organoids and preventing the glass from fogging and affecting the lighting effect.

[0075] In a preferred embodiment, the locking mechanism 21 includes two locking seats 210 connected to the rotating cover 20, a rotating shaft 211 rotatably connected to the locking seats 210, a handle 212 connected to the rotating shaft 211, a limiting block 213 arranged on the side of the handle 212, a locking hook 214 arranged at the bottom of the handle 212, and a torsion spring 215 sleeved on the rotating shaft 211 and located between the limiting block 213 and the locking seat 210; an inverted hook 14 for cooperating with the locking hook 214 is arranged at one end of the base platform 10. Under the elastic force of the torsion spring 215, the locking hook 214 can hook the inverted hook 14 to achieve locking; when opening the cover, rotating the handle 212 around the rotating shaft 211 can complete the unlocking operation; the torsion spring 215 provides a torsional force for the reset of the handle 212 to ensure that the locking mechanism 21 can maintain a reliable locking state.

[0076] In a preferred embodiment, the organoid culture device further includes a first upper temperature control component 40 arranged on the translation cover 31 and a second upper temperature control component 41 arranged on the rotating cover 20. The first upper temperature control component 40 includes a first upper heating belt, a first upper temperature sensor and a first over-temperature sensor 43 arranged on the translation cover 31; the second upper temperature control component 41 includes a second upper heating belt 44, a second upper temperature sensor and a second over-temperature sensor 45 arranged on the rotating cover 20.

[0077] The first upper heating belt and the second upper heating belt 44 respectively realize the heating functions for the translation cover 31 and the rotation cover 20. The first upper temperature sensor monitors the temperature of the translation cover 31 in real time to keep it stable within a reasonable range. The first upper overtemperature sensor can cut off the heating function of the first heating belt when the first upper temperature sensor detects that the temperature is too high, preventing the organoids from being affected by excessive temperature. The second upper temperature control component 41 realizes the temperature control of the rotation cover 20, and the principle is the same, so it will not be elaborated here. In a preferred embodiment, heat insulation cotton is attached to the heating belt to reduce the influence of external temperature changes on the temperature in the culture chamber and improve the heating efficiency.

[0078] In a preferred embodiment, the organoid culture device further includes a communication connection component 5, which includes a communication module provided on the rotation cover 20, a lower circuit board 50 connected to the communication module, an upper circuit board 51 connected to the translation cover 31, and a plurality of elastic pieces 52 provided on the upper circuit board 51. The first upper temperature sensor and the first upper overtemperature sensor are both communicatively connected to the upper circuit board 51. When the translation cover 31 is closed, the elastic piece 52 contacts and conducts with the corresponding contact on the lower circuit board 50, and is connected to the external control element; when the translation cover 31 is opened, the elastic piece 52 moves away from the lower circuit board 50 as the translation cover 31 moves, and the connection between the first upper temperature sensor, the first upper overtemperature sensor and the external control element is disconnected.

[0079] A conductive ball plunger 53 is also connected to the translation cover 31. The first upper heating belt is electrically connected to the conductive ball plunger 53. When the translation cover 31 is closed, the lower end of the conductive ball plunger 53 contacts and conducts with the corresponding contact on the lower circuit board 50, enabling heating; when the cover is opened, the conductive ball plunger 53 moves with the translation cover 31, and the heating of the heating belt is correspondingly disconnected.

[0080] In a preferred embodiment, the organoid culture device further includes a lower temperature control component 42 provided on the base platform 10. The lower temperature control component 42 includes a lower heating belt 46, a lower temperature sensor and a lower overtemperature sensor provided on the base platform 10; the principle is the same as that of the first upper temperature control component 40, so it will not be elaborated here.

[0081] In a preferred embodiment, the organoid culture device further includes a humidity control component 6. The humidity control component 6 includes a humidifying bottle 60 provided on the base platform 10, a humidifying container 61 communicated with the humidifying bottle 60 through a humidifying pipeline, an air inlet needle 62 with an air outlet extending into the interior of the humidifying container 61, and a humidity sensor 63 provided above the humidifying container 61. External gas enters through the air inlet needle 62 and is then transported to the bottom of the humidifying container 61 filled with liquid, and the gas is humidified by freely diffusing in the liquid to realize the humidification of the gas entering the culture chamber, and the humidity sensor 63 detects the humidity inside.

[0082] In a preferred embodiment, the organoid culture device further includes a liquid path heating member 7 disposed on the base platform 10. The liquid path heating member 7 includes a plurality of liquid inlet pipes 70 horizontally disposed on the base platform 10 and a heating plate 71 covering the liquid inlet pipes 70. A pressure pipe groove 72 matching the liquid inlet pipes 70 is formed on the bottom surface of the heating plate 71. Among them, the heating plate 71 can generate heat by itself or only conduct heat without generating heat. In this embodiment, the heating plate 71 only conducts heat, and it transfers the heat generated by the lower heating belt 46 on the base platform 10 to the liquid inlet pipes 70 to realize the heating of the incoming liquid. The heated culture medium is then transported to the culture dish 11 to ensure the temperature requirements for culturing. The pressure pipe groove 72 is designed in a semicircular shape to increase the contact area with the liquid pipe and improve the heating efficiency.

[0083] In a preferred embodiment, a plurality of observation holes 12 are formed in the base platform 10 at a position below the culture dish 11, and glass sheets are provided on the observation holes 12 to facilitate the real-time observation of the organoids during the culture process.

[0084] A hinge 13 for connecting with the rotating cover 20 is provided at the end of the base platform 10. In this embodiment, it is a torque hinge 13, which can make the rotating cover 20 stop at any position during the opening process, facilitating the operation inside the culture chamber.

[0085] In a preferred embodiment, the organoid culture device further includes an upper switch cover monitoring component 8 and a lower switch cover monitoring component 9. The upper switch cover monitoring component 8 includes an upper groove-shaped optocoupler 80 disposed on the rotating cover 20 and an upper optocoupler blocking piece 81 disposed on the translation cover 31 for cooperating with the upper groove-shaped optocoupler 80; the lower switch cover monitoring component 9 includes a lower groove-shaped optocoupler 90 disposed on the base platform 10 and a lower optocoupler positioning pin (not shown in the figure) disposed on the rotating cover 20 for cooperating with the lower groove-shaped optocoupler 90;

[0086] When the translation cover 31 is opened, the upper optocoupler blocking piece 81 disengages from the upper groove-shaped optocoupler 80 and does not block the optocoupler light. Thus, the state of opening and closing the cover can be judged to avoid the influence of the opening of the upper cover of the culture chamber on the organoid culture. The judgment principle for the opening and closing of the rotating cover 20 is the same and will not be elaborated here.

[0087] Although the embodiments of the present invention have been disclosed as above, it is not limited to only the applications listed in the specification and embodiments. It can be fully applied to various fields suitable for the present invention. For those familiar with the field, additional modifications can be easily made. Therefore, without departing from the general concept defined by the claims and the equivalent scope, the present invention is not limited to specific details.

Claims

1. An organoid culture device, characterized in that, Comprising: A platform component, which includes a base platform and a culture dish provided on the base platform for placing organoids; A lid-lifting component, which includes a rotating lid rotatably connected to the base platform around the Y axis, a locking mechanism provided on the rotating lid for locking the rotating lid on the base platform, and an operation window opened in the middle of the rotating lid. When the rotating lid is buckled on the base platform, the operation window is above the culture dish; And a lid-shifting component, which includes a pressing lid mechanism connected to the rotating lid and a translation lid slidably arranged along the X axis between the rotating lid and the pressing lid mechanism. The translation lid is driven to reciprocate along the X axis to switch between an open-lid state and a closed-lid state. In the open-lid state, the translation lid leaves above the operation window, and in the closed-lid state, the translation lid completely covers the operation window; The pressing lid mechanism includes two pressing lid bars with an L-shaped cross-section symmetrically connected to the rotating lid about the X axis, and a plurality of pressing lid ball plungers arranged at intervals along the X direction on the pressing lid bars; A gap between the pressing lid bar and the rotating lid forms a translation chute for the two sides of the translation lid to be inserted and fitted. The ball head at the bottom of the pressing lid ball plunger extends downward into the translation chute; Both sides of the translation lid along the X direction are formed with translation slide rails. Both ends of the translation slide rails along the X direction protrude from the translation lid. The surface of the translation slide rails is provided with a V-shaped groove for the ball head of the pressing lid ball plunger to be fitted and pushed into. The bottom surface of one end of the translation slide rail close to the locking mechanism is recessed upward to form a guiding surface, and the guiding surface and the bottom surface of the translation slide rail are transitioned through an arc slope; A top lid component for cooperating with the translation slide rail is arranged on the inner side of the pressing lid bar. The top lid component includes a top lid shaft connected to the pressing lid bar and a bearing rotatably connected to the top lid shaft. The bearing is used to generate an upward pressing force on the bottom surface of the translation slide rail when the translation lid reciprocates along the X axis; 2. The organoid culture device according to claim 1, wherein A push lid hole is opened on the translation lid, and the translation lid is slid by applying a thrust in the X-axis direction to the translation lid; A plurality of lighting holes are opened on the translation lid, and double-layer glass is provided on the lighting holes; 3. The organoid culture device according to claim 1, characterized in that, The locking mechanism includes two locking seats connected to the rotating lid, a rotating shaft rotatably connected to the locking seats, a handle connected to the rotating shaft, a limiting block arranged on the side of the handle, a locking hook arranged at the bottom of the handle, and a torsion spring sleeved on the rotating shaft and located between the limiting block and the locking seat; 4. The organoid culture device according to claim 1, characterized in that, It further includes a first upper temperature control component arranged on the translation lid and a second upper temperature control component arranged on the rotating lid. The first upper temperature control component includes a first upper heating tape, a first upper temperature sensor, and a first over-temperature sensor arranged on the translation lid; The second upper temperature control component includes a second upper heating tape, a second upper temperature sensor, and a second over-temperature sensor arranged on the rotating lid.

5. The organoid culture device according to claim 4, wherein It further includes a communication connection component. The communication connection component includes a communication module provided on the rotary cover, a lower circuit board connected to the communication module, an upper circuit board connected to the translation cover, and a plurality of elastic pieces provided on the upper circuit board. The first upper temperature sensor and the first over-temperature sensor are both communicatively connected to the upper circuit board. When the translation cover is closed, the elastic pieces come into contact with corresponding contacts on the lower circuit board to conduct electricity; A conductive ball plunger is further connected to the translation cover. The first upper heating tape is electrically connected to the conductive ball plunger. When the translation cover is closed, the lower end of the conductive ball plunger comes into contact with a corresponding contact on the lower circuit board to conduct electricity.

6. The organoid culture device according to claim 1, characterized in that, It further includes a lower temperature control component provided on the base platform. The lower temperature control component includes a lower heating tape, a lower temperature sensor, and a lower over-temperature sensor provided on the base platform.

7. The organoid culture device according to claim 1, characterized in that, It further includes a humidity control component. The humidity control component includes a humidifying bottle provided on the base platform, a humidifying container communicated with the humidifying bottle through a humidifying pipeline, an intake needle with an air outlet extending into the interior of the humidifying container, and a humidity sensor provided above the humidifying container.

8. The organoid culture device according to claim 1, characterized in that, It further includes a liquid path heating element provided on the base platform. The liquid path heating element includes a plurality of liquid inlet pipelines horizontally provided on the base platform and a heating plate covering the liquid inlet pipelines. The bottom surface of the heating plate is provided with a pressing groove matching the liquid inlet pipelines.

9. The organoid culture device according to claim 3, characterized in that, A plurality of observation holes are provided in the base platform at a position below the culture dish, and glass sheets are provided on the observation holes; One end of the base platform is provided with a hinge for connecting to the rotary cover, and the other end of the base platform on the opposite side of the hinge is provided with a barb for cooperating with the locking hook.

10. The organoid culture device according to claim 1, wherein It further includes an upper switch cover monitoring component and a lower switch cover monitoring component. The upper switch cover monitoring component includes an upper groove-shaped optocoupler provided on the rotary cover and an upper optocoupler baffle provided on the translation cover for cooperating with the upper groove-shaped optocoupler; The lower switch cover monitoring component includes a lower groove-shaped optocoupler provided on the base platform and a lower optocoupler positioning pin provided on the rotary cover for cooperating with the lower groove-shaped optocoupler.

11. The organoid culture device according to claim 1, wherein, An annular groove is provided on the bottom surface of the rotary cover, and a sealing ring is provided in the annular groove.

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