A method for detecting perfluorinated compounds using a sensor array composed of dyes

Abstract

The invention discloses a method for detecting perfluorinated compounds by using a sensor array composed of dyes, comprising the following steps: S1, preparing a fluorescent dye solution: preparing a fluorescein diluent, a coumarin 7 diluent, and a cyanine diluent; S2, preparing a pollutant solution: preparing diluted PFBA solution, PFOA solution, PFDA solution, PFUdA solution, and PFDoA solution; S3, constructing a fluorescent sensor array and detecting perfluorinated compounds: placing a sample in a fluorescence spectrometer for detection. The dyes used in the detection method of the invention are easy to obtain and have strong fluorescence stability. The sensor array can detect perfluorinated compounds with similar structures, and the operation is simple.

Description

Technical Field

[0001] The present invention relates to the technical field of sensor array detection, in particular to a method for detecting perfluorinated compounds by utilizing a sensor array composed of dyes. Background Art

[0002] Per- and polyfluorinated substances (PFAS) are a class of synthetic aliphatic hydrocarbon compounds. Due to their unique inertness, hydrophobicity, oleophobicity, excellent lubricity, and stain repellency, PFAS are widely used in textiles, coatings, chemicals, paper, and packaging. In the food contact material sector, PFAS are most widely used in the production of polytetrafluoroethylene (PTFE), non-stick coatings for cookware, and as additives to paper products used to hold hot, greasy foods. N-ethylperfluorooctanesulfonamidoethanol (EtFOSE) is widely used in papermaking. Because perfluorinated compounds are excellent surfactants, they are also frequently added to inks to help disperse their components. Currently, perfluorinated compounds used in paper coatings or additives are primarily low-molecular-weight (C6, C8, etc.) or high-molecular-weight (such as C10 and C12) perfluorinated compounds with a backbone containing certain side chains.

[0003] The carbon monoxide (CF) in PFAS makes these compounds difficult to degrade. Once released into the environment, they can persist stably in nature, continuously transferring and accumulating within the food chain. Toxicological studies have shown that long-chain PFAS can cause a variety of diseases, leading many international fluorochemical manufacturers to conduct research on alternatives to short-chain PFASs. However, short-chain PFASs, like long-chain PFASs, have toxic effects on the liver and thyroid gland, but require higher doses to achieve similar effects. Therefore, research on PFAS requires a comprehensive focus on the use of both long-chain and short-chain PFASs.

[0004] The design of sensor arrays is inspired by the simulation of the mammalian olfactory system. Research has shown that the olfactory system is not characterized by a single receptor responding to a single specific analyte, but rather by a single receptor responding to multiple analytes simultaneously, and vice versa. Therefore, array sensing can produce highly selective sensor elements without complex design and synthesis processes. Each element can respond to multiple analytes, thus reducing the requirements for sensor design and expanding the range of analytes that can be detected by the sensing platform. By collecting the different response information of each sensor element to each analyte, a unique response pattern can be obtained, which can then be used to identify the analyte, achieving the goal of simultaneously detecting and distinguishing multiple substances in complex environments. Sensor array methods have attracted the attention and favor of researchers due to their advantages in detecting complex samples.

[0005] Therefore, the present invention utilizes the superiority of sensor array detection to detect perfluorinated compounds. Summary of the Invention

[0006] In order to solve the above technical problems, the present invention provides a method for detecting perfluorinated compounds using a sensor array composed of dyes.

[0007] The technical solution of the present invention is: a method for detecting perfluorinated compounds using a sensor array composed of dyes, comprising the following steps:

[0008] S1. Preparation of fluorescent dye solution:

[0009] Three fluorescent dye solutions, namely, fluorescein solution, coumarin 7 solution, and cyanine solution, each with a concentration of 500 mg / L, were prepared respectively. The fluorescein solution was diluted 3000 times, the coumarin 7 solution was diluted 1500 times, and the cyanine solution was diluted 375 times to obtain fluorescein dilution solution, coumarin 7 dilution solution, and cyanine dilution solution, respectively.

[0010] S2. Preparation of pollutant solution:

[0011] Prepare five pollutant solutions containing perfluorinated compounds, each with a concentration of 10 g / L, and then dilute the five pollutant solutions to 250 mg / L to obtain five pollutant dilution solutions for use;

[0012] S3. Construction of fluorescence sensor array:

[0013] S3-1. Fluorescein fluorescence sensing:

[0014] 1.5 mL of the fluorescein dilution obtained in step S1 was added to each of five 5 mL centrifuge tubes with caps. 0, 20, 50, 100, and 150 μL of one of the pollutant dilutions obtained in step S2 were then added to each of the five centrifuge tubes with caps. Finally, ultrapure water was added to bring the volume of the mixed solution in the centrifuge tubes with caps to 2.5 mL. The steps and parameters for preparing the mixed solution were repeated to prepare a mixed solution of the fluorescein dilution and the remaining four pollutant dilutions described in step S2. Five parallel samples of the mixed solutions containing pollutants of various concentrations were prepared, thereby obtaining 125 fluorescein fluorescence sensing samples.

[0015] S3-2, Coumarin 7 fluorescence sensing:

[0016] Then, the coumarin 7 dilution obtained in step S1 was used to prepare 125 coumarin 7 fluorescence sensing samples using the same steps and parameters as step S3-1.

[0017] S3-3, Cyanine fluorescence sensing:

[0018] Then, the cyanine dilution obtained in step S1 was used to prepare 125 cyanine fluorescence sensing samples using the same steps and parameters as step S3-1.

[0019] S4. Detection of perfluorinated compounds:

[0020] The fluorescein fluorescence sensing sample, coumarin 7 fluorescence sensing sample and cyanine fluorescence sensing sample prepared in step S3 were allowed to stand for 10 to 300 minutes and then sequentially placed in a fluorescence spectrometer to detect the fluorescence response intensity of the perfluorinated compounds.

[0021] Furthermore, in step S1, the preparation method of the fluorescein solution, coumarin 7 solution, and cyanine solution is as follows: 10 mg of fluorescein solid, coumarin 7 solid, and cyanine solid are respectively dissolved in 20 mL of an organic solvent.

[0022] Description: Fluorescein, coumarin 7, and cyanine are all common and readily available fluorescent dyes that are easily soluble in organic solvents, and the preparation methods of their corresponding solutions are simple. Fluorescein has a wide detection range; coumarin 7 is relatively stable in terms of photostability; and cyanine has bright fluorescence that is easy to observe.

[0023] Furthermore, in step S2, the five pollutant solutions containing perfluorinated compounds are PFBA solution, PFOA solution, PFDA solution, PFUdA solution, and PFDoA solution.

[0024] Note: The above five pollutant solutions are all perfluoroalkyl compounds. These perfluoro compounds have similar structures. In some traditional detection methods, it is difficult to distinguish and detect them. They are prone to overlap and aggregation, which affects the detection results. Therefore, the method of the present invention uses these five pollutant solutions to demonstrate the excellence of this detection method.

[0025] Furthermore, the preparation method of the PFBA solution, PFOA solution, PFDA solution, PFUdA solution, and PFDoA solution is as follows: 200 mg of PFBA solid, PFOA solid, PFDA solid, PFUdA solid, and PFDoA solid are taken and dissolved in 20 mL of an organic solvent respectively.

[0026] Note: The above-mentioned pollutants are easily soluble in organic solvents. The preparation method for preparing the pollutant solution is simple to operate and easy to control the concentration.

[0027] Furthermore, the organic solvent in step S1 is an acetonitrile solution.

[0028] Description: Acetonitrile is a highly polar organic solvent that has good solubility for oils, inorganic salts, organic matter and high molecular weight compounds.

[0029] Furthermore, the dilution in step S1 and step S2 is performed by adding ultrapure water.

[0030] Note: Ultrapure water contains almost no impurities other than water molecules, and no bacteria, viruses, chlorinated dioxins or other organic matter. Therefore, it will not produce any other reactions when used for dilution and will not affect the concentration of fluorescent dyes or pollutants.

[0031] Furthermore, in step S3-4, the excitation wavelength of the fluorescence spectrometer is 240~550nm, and the positions of the fluorescence intensities taken are: fluorescein: (Ex, Em): (480nm, 512nm); coumarin 7: (Ex, Em): (450nm, 503nm); cyanine: (Ex, Em): (315nm, 406nm).

[0032] Note: The fluorescence intensity at the above wavelength is the largest, and at the position of the fluorescence wavelength, the peak position has no displacement or very little displacement, which is conducive to observing the detection status of the sample.

[0033] Furthermore, the organic solvent in step S2 is a methanol solution.

[0034] Note: Methanol is an important organic solvent and its dissolving performance is better than ethanol.

[0035] Furthermore, before adding ultrapure water in step S3, the pollutant solution is first activated: an activator accounting for 1.5-3.5% of the volume of each pollutant solution is added to the five pollutant solutions respectively under an argon environment with a pressure of 0.1-0.3 MPa. After the addition is completed, the solution is placed at 130-145° C. for activation for 20-30 minutes, and microwave treatment is applied simultaneously during the activation process, with a microwave power of 25-45 kW.

[0036] Note: In an argon environment, other impurities can be prevented from entering the pollutant solution along with the activator. Adding a small amount of activator can increase the activity of the pollutants, making the pollutants easier to be labeled and detected by fluorescent dyes in subsequent tests. At a slightly higher temperature, the activation ability of the activator can be stimulated, further enhancing the activity of the pollutants. Simultaneous microwave treatment can allow the activator to fully and evenly act on most pollutants, thereby improving their activity.

[0037] Furthermore, the activator consists of 10-12 wt.% of isophenyl hydroperoxide, 3-5 wt.% of diethylene glycol monoallyl ether and the balance of ethylenediamine phosphate.

[0038] Description: The activator is prepared by mixing commonly used ethylenediamine phosphate for activation, isophenylhydroperoxide with catalytic effect and a small amount of glycol monoallyl ether. Compared with traditional activators, the activation conditions of the activator are reduced and the activation ability of the activator is enhanced.

[0039] The beneficial effects of the present invention are:

[0040] (1) The fluorescent dye used in the detection method of the present invention is easy to obtain and has strong fluorescence stability. The fluorescent dye is used to perform fluorescence detection on pollutants. This method has high sensitivity and is easy to operate. It can also solve the limitation that traditional fluorescence detection is only applicable to single or multiple pollutants, and the detection results are more accurate.

[0041] (2) The detection method of the present invention adopts a sensor array method, which can detect multiple pollutants, and can detect and distinguish pollutants with similar structures. It can also detect different concentrations of the same pollutant, thereby expanding the detection range of pollutants and enhancing the applicability of the detection method.

[0042] (3) The detection method of the present invention uses three fluorescent dyes to form a sensor array to detect perfluorinated compounds with similar structures, which improves the shortcomings of other traditional detection methods that can only detect one structure or one concentration, and realizes the detection of multiple concentrations of a single perfluorinated compound and a single concentration of multiple perfluorinated compounds. BRIEF DESCRIPTION OF THE DRAWINGS

[0043] Figure 1 is the original fluorescence response matrix diagram of the perfluorinated compound at a concentration of 5 mg / L according to the detection method of the present invention;

[0044] Figure 2 It is a standard score graph of perfluorinated compounds of the same concentration using the linear discriminant analysis (LDA) of the detection method of the present invention;

[0045] Figure 3 This is a standard score graph of perfluorinated compounds at different concentrations using the linear discriminant analysis (LDA) of the detection method of the present invention. DETAILED DESCRIPTION

[0046] The present invention will be further described in detail below in conjunction with specific implementation methods to better demonstrate the advantages of the present invention. Example 1

[0047] A method for detecting perfluorinated compounds using a sensor array composed of dyes, comprising the following steps:

[0048] S1. Preparation of fluorescent dye solution:

[0049] Dissolve 10 mg of fluorescein solid, coumarin 7 solid, and cyanine solid in 20 mL of acetonitrile solution to prepare 500 mg / L fluorescein solution, coumarin 7 solution, and cyanine solution, respectively. Then, dilute the fluorescein solution 3000 times, the coumarin 7 solution 1500 times, and the cyanine solution 375 times to obtain fluorescein dilution solution, coumarin 7 dilution solution, and cyanine dilution solution, respectively.

[0050] S2. Preparation of pollutant solution:

[0051] 200 mg of PFBA solid, PFOA solid, PFDA solid, PFUdA solid, and PFDoA solid were taken respectively and dissolved in 20 mL of methanol solution to prepare five pollutant solutions with a concentration of 10 g / L each: PFBA solution, PFOA solution, PFDA solution, PFUdA solution, and PFDoA solution. The five pollutant solutions were then diluted to 250 mg / L with ultrapure water to obtain five pollutant dilution solutions for use;

[0052] S3. Construction of fluorescence sensor array:

[0053] S3-1. Fluorescein fluorescence sensing:

[0054] Add 1.5 mL of the fluorescein dilution obtained in step S1 to each of five 5 mL centrifuge tubes with caps. Then, add 0, 20, 50, 100, and 150 μL of the PFBA dilution obtained in step S2 to each of the five 5 mL centrifuge tubes with caps, respectively. Finally, add ultrapure water to the centrifuge tubes with caps until the volume of the mixed solution is 2.5 mL. The concentration of fluorescein in the mixed solution is 0.1 mg / L, and the concentrations of PFBA are 0 mg / L, 2 mg / L, 5 mg / L, 10 mg / L, and 15 mg / L, respectively.

[0055] Repeat the steps and parameters for preparing the mixed solution to prepare a mixed solution of the fluorescein dilution solution and the other four pollutant dilution solutions described in step S2; and prepare five parallel samples of each mixed solution containing pollutants at the same concentration, thereby obtaining 125 fluorescein fluorescence sensing samples;

[0056] S3-2, Coumarin 7 fluorescence sensing:

[0057] The coumarin 7 dilution obtained in step S1 was then used to prepare 125 coumarin 7 fluorescence sensing samples using the same steps and parameters as in step S3-1. The concentration of coumarin 7 in the mixed solution was 0.2 mg / L, and the concentrations of various pollutants were the same as in step S3-1.

[0058] S3-3, Cyanine fluorescence sensing:

[0059] Then, the cyanine diluted solution obtained in step S1 was used to prepare 125 cyanine fluorescence sensing samples using the same steps and parameters as step S3-1. The cyanine concentration in the mixed solution was 0.8 mg / L, and the concentrations of various pollutants were the same as in step S3-1.

[0060] S4. Detection of perfluorinated compounds:

[0061] The fluorescein fluorescence sensing sample, coumarin 7 fluorescence sensing sample, and cyanine fluorescence sensing sample prepared in step S3 were allowed to stand for 150 minutes and then placed in a fluorescence spectrometer to detect the fluorescence response intensity of the perfluorinated compounds;

[0062] The excitation wavelength of the fluorescence spectrometer is 240~550nm, and the fluorescence intensity is measured at the following positions: Fluorescein: (Ex, Em): (480nm, 512nm); Coumarin 7: (Ex, Em): (450nm, 503nm); Cyanine: (Ex, Em): (315nm, 406nm). Example 2

[0063] The difference between this embodiment and embodiment 1 is that in step S4, the three fluorescent sensing samples are left to stand for 10 minutes. Example 3

[0064] The difference between this embodiment and embodiment 1 is that, in step S4, the three fluorescent sensing samples are left to stand for 300 minutes. Example 4

[0065] This embodiment differs from embodiment 1 in that, in step S3, before adding ultrapure water in step S3, the mixed solution to which ultrapure water is not added is first activated: an activator accounting for 2.0% of the volume of the mixed solution to which ultrapure water is not added is added is added to each of the five pollutant solutions under an argon atmosphere at a pressure of 0.2 MPa. After the addition is completed, the mixture is placed at 138° C. for activation for 25 minutes, and microwave treatment is simultaneously applied during the activation process, wherein the microwave power is 30 kW.

[0066] The activator consists of 11 wt.% of isophenyl hydroperoxide, 4 wt.% of diethylene glycol monoallyl ether and the balance of ethylenediamine phosphate. Example 5

[0067] The difference between this embodiment and embodiment 4 is that the gas pressure in the argon environment is 0.1 MPa. Example 6

[0068] The difference between this embodiment and embodiment 4 is that the gas pressure in the argon environment is 0.3 MPa. Example 7

[0069] The difference between this embodiment and embodiment 4 is that the amount of the activator added is 1.5% of the volume of the mixed solution without ultrapure water. Example 8

[0070] The difference between this embodiment and embodiment 4 is that the amount of the activator added is 2.5% of the volume of the mixed solution without ultrapure water. Example 9

[0071] The difference between this embodiment and embodiment 4 is that the activation temperature is 130° C. and the activation time is 20 min. Example 10

[0072] The difference between this embodiment and embodiment 4 is that the activation temperature is 145° C. and the activation time is 30 min. Example 11

[0073] The difference between this embodiment and embodiment 4 is that the power of the microwave is 25KW. Example 12

[0074] The difference between this embodiment and embodiment 4 is that the power of the microwave is 45KW. Example 13

[0075] This embodiment differs from embodiment 4 in that the activator consists of 12 wt.% of isophenylpropane hydroperoxide, 3 wt.% of diethylene glycol monoallyl ether, and the balance of ethylenediamine phosphate. Example 14

[0076] This embodiment differs from embodiment 4 in that the activator consists of 10 wt.% of isophenyl hydroperoxide, 5 wt.% of diethylene glycol monoallyl ether, and the balance of ethylenediamine phosphate.

[0077] Experimental example

[0078] The change in fluorescence is defined as F / F0, where F0 is the fluorescence intensity in the absence of perfluorinated compounds and F is the fluorescence intensity in the presence of perfluorinated compounds. F / F0 is used to represent the effect of the five perfluorinated compounds on the fluorescence of the three luminescent dyes.

[0079] according to Figure 1As shown, a heat map is made based on the original data of F / F0, and the degree of fluorescence intensity response is distinguished according to the color card on the right side of the figure. The color of each grid represents the degree of fluorescence intensity response. The lower the value, the smaller the F / F0 and the greater the response. The concentration of the five pollutants is 5 mg / L. It can be seen that the five pollutants respond to the three fluorescent dyes respectively, and the fluorescence response intensity of the same pollutant to different fluorescent dyes is different, and the fluorescence response intensity of different pollutants to the same fluorescent dye is also different. It can be seen from the figure that PFBA and PFDA have the strongest fluorescence response intensity to fluorescein, PFUdA has the strongest fluorescence response intensity to cyanine, and PFUdA and PFDA have the strongest fluorescence response intensity to coumarin 7.

[0080] according to Figure 2 As shown in the figure, the LDA score value is calculated based on the original data of F / F0, and then a graph (95% confidence interval) is drawn: the four graphs represent the situation of five pollutants at four identical pollutant concentrations. It can be seen from the figure that different pollutants at the same concentration are clearly separated and do not overlap or aggregate with each other. It can be seen that at each concentration, the five pollutants have different effects on the fluorescence intensity of the dye, thus indicating that the detection method of the present invention can detect and distinguish different types of pollutants;

[0081] according to Figure 3 As shown, the LDA score value is calculated based on the original data of F / F0, and then a graph is drawn (95% confidence interval): The five graphs represent five pollutants, each of which has different concentrations. The same pollutants at different concentrations are also clearly separated, without overlap or aggregation. It can be seen that the same pollutant has different effects on the fluorescence intensity of the dye at different concentrations. Therefore, it is shown that the detection method of the present invention can detect and distinguish the same pollutants at different concentrations.

[0082] Based on the test results of each embodiment, 5 samples of each embodiment were taken to test the effects of the five perfluorinated compounds on the fluorescence of the three luminescent dyes. The average value of the detection rate measurement results of the 5 samples of each embodiment was taken as the detection rate measurement result of the embodiment. The specific exploration is as follows:

[0083] 1. The detection parameters of step S4, i.e., Examples 1-3, have little effect on the detection rate of perfluorinated compounds and are conventional adjustment parameters, so they will not be discussed in detail.

[0084] 2. Investigate the effect of activation treatment on the average detection rate of perfluorinated compounds at a concentration of 5 mg / L.

[0085] Examples 4-14 and control examples 1-3 were used for experimental comparison, and the results are shown in Table 1:

[0086] Table 1 Average detection improvement rate of perfluorinated compounds in Examples and Control Examples compared with Example 1

[0087]

[0088] The difference between Comparative Example 1 and Example 4 is that the activation treatment is carried out under indoor atmospheric pressure;

[0089] Comparative Example 2 differs from Example 4 in that microwave treatment is not applied;

[0090] The difference between Control Example 3 and Example 4 is that the activator consists of 11 wt.% of isopropyl hydroperoxide and the balance of ethylenediamine phosphate;

[0091] As shown in Table 1, the detection improvement rates of Examples 4-14 and Comparative Examples 1-3 are all positive, indicating that they have all been improved to a certain extent. However, in the case where the comparative examples lack an argon environment, lack microwave treatment, and lack diethylene glycol monoallyl ether, the detection improvement rates are all lower than those of Examples 4-14.

[0092] The detection improvement rates of Examples 4 and 10 are relatively high, but Example 10 requires a higher activation temperature and a longer activation time than Example 4, and the improvement in the detection improvement rate is small; and too low or too high gas pressure, too much or too little addition of activator, too low or too high microwave power, and too little or too much proportion of ethanol loss value will all reduce the detection improvement rate. Therefore, from an economic perspective, the detection improvement rate under the parameters of Example 4 is relatively better.

Claims

1. A method for detecting perfluorinated compounds using a sensor array composed of dyes, characterized in that: The following steps are involved: S1. Preparation of fluorescent dye solution: Three fluorescent dye solutions, namely, fluorescein solution, coumarin 7 solution, and cyanine solution, each with a concentration of 500 mg / L, were prepared respectively. The fluorescein solution was diluted 3000 times, the coumarin 7 solution was diluted 1500 times, and the cyanine solution was diluted 375 times to obtain fluorescein dilution solution, coumarin 7 dilution solution, and cyanine dilution solution, respectively. S2. Preparation of pollutant solution: Prepare five pollutant solutions containing perfluorinated compounds, each with a concentration of 10 g / L, respectively. The five pollutant solutions containing perfluorinated compounds are PFBA solution, PFOA solution, PFDA solution, PFUdA solution, and PFDoA solution. Then, dilute the five pollutant solutions to 250 mg / L to obtain five pollutant dilution solutions for use. S3. Construction of fluorescence sensor array: S3-1. Fluorescein fluorescence sensing: 1.5 mL of the fluorescein dilution obtained in step S1 was added to each of five 5 mL centrifuge tubes with caps. 0, 20, 50, 100, and 150 μL of one of the pollutant dilutions obtained in step S2 were then added to each of the five centrifuge tubes with caps. Finally, ultrapure water was added to bring the volume of the mixed solution in the centrifuge tubes with caps to 2.5 mL. The steps and parameters for preparing the mixed solution were repeated to prepare a mixed solution of the fluorescein dilution and the remaining four pollutant dilutions described in step S2. Five parallel samples of the mixed solutions containing pollutants of various concentrations were prepared, thereby obtaining 125 fluorescein fluorescence sensing samples. S3-2, Coumarin 7 fluorescence sensing: Then, the coumarin 7 dilution obtained in step S1 was used to prepare 125 coumarin 7 fluorescence sensing samples using the same steps and parameters as step S3-1. S3-3, Cyanine fluorescence sensing: Then, the cyanine dilution obtained in step S1 was used to prepare 125 cyanine fluorescence sensing samples using the same steps and parameters as step S3-1. S4. Detection of perfluorinated compounds: The fluorescein fluorescence sensing sample, coumarin 7 fluorescence sensing sample and cyanine fluorescence sensing sample prepared in step S3 were allowed to stand for 10 to 300 minutes and then sequentially placed in a fluorescence spectrometer to detect the fluorescence response intensity of the perfluorinated compounds.

2. The method for detecting perfluorinated compounds using a sensor array composed of dyes according to claim 1, wherein: In step S1, the preparation method of the fluorescein solution, coumarin 7 solution and cyanine solution is as follows: 10 mg of fluorescein solid, coumarin 7 solid and cyanine solid are dissolved in 20 mL of organic solvent respectively.

3. The method for detecting perfluorinated compounds using a sensor array composed of dyes according to claim 1, characterized in that: The PFBA solution, PFOA solution, PFDA solution, PFUdA solution, and PFDoA solution were prepared by taking 200 mg of PFBA solid, PFOA solid, PFDA solid, PFUdA solid, and PFDoA solid, respectively, and dissolving them in 20 mL of an organic solvent.

4. The method for detecting perfluorinated compounds using a sensor array composed of dyes according to claim 2, wherein: The organic solvent in step S1 is acetonitrile solution.

5. The method for detecting perfluorinated compounds using a sensor array composed of dyes according to claim 1, characterized in that: The dilution in step S1 and step S2 is performed by adding ultrapure water.

6. The method for detecting perfluorinated compounds using a sensor array composed of dyes according to claim 1, characterized in that: In step S3-4, the excitation wavelength of the fluorescence spectrometer is 240-550 nm, and the fluorescence intensity is measured at the following positions: fluorescein: (Ex, Em): (480 nm, 512 nm); coumarin 7: (Ex, Em): (450 nm, 503 nm); cyanine: (Ex, Em): (315 nm, 406 nm).

7. The method for detecting perfluorinated compounds using a sensor array composed of dyes according to claim 3, characterized in that: The organic solvent is methanol solution.

8. The method for detecting perfluorinated compounds using a sensor array composed of dyes according to claim 1, characterized in that: Before adding ultrapure water in step S3, the mixed solution to which ultrapure water has not been added is first activated: an activator accounting for 1.5-3.5% of the volume of the mixed solution to which ultrapure water has not been added is added to each of the five pollutant solutions in an argon environment with a pressure of 0.1-0.3 MPa. After the addition is completed, the solutions are placed at 130-145° C. for activation for 20-30 minutes, and microwave treatment is simultaneously applied during the activation process, with a microwave power of 25-45 kW.

9. The method for detecting perfluorinated compounds using a sensor array composed of dyes according to claim 8, characterized in that: The activator consists of 10-12 wt.% of isophenyl hydroperoxide, 3-5 wt.% of diethylene glycol monoallyl ether and the balance of ethylenediamine phosphate.

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