Pressure control line and method

Abstract

The application discloses a pressure control pipeline and method, which comprises a washing device, a detection device, a liquid packaging device and a gas control device, the communication pipeline of the liquid packaging device is connected with the liquid inlet of the washing device, and the communication pipeline of the gas control device is connected with the air inlet of the washing device; the detection device comprises a filter, a first sensor assembly and a first electromagnetic valve, the electromagnetic valve is connected with the first sensor assembly, and the communication pipeline of the first sensor assembly is connected with the filter; the communication pipeline of the filter is communicated with the pipeline connected with the liquid inlet of the washing device; the gas control device controls the increase of the air pressure below the washing device, the liquid above the washing device is pressed and discharged from the liquid inlet to the liquid packaging device along the pipeline, the gas in the pipeline is extruded, the first sensor assembly detects the increase of the air pressure in the communicated pipeline, sends a signal to the electromagnetic valve, the normally closed valve port in the electromagnetic valve is conducted, and the gas at the other end of the normally closed valve port is supplemented into the pipeline.

Description

TECHNICAL FIELD

[0001] The present application relates to the field of cell processing, in particular to a pressure control pipeline and method. BACKGROUND

[0002] In the process of cell liquid concentration and washing, biological cell liquid is processed in the centrifugal mechanism, and the processed liquid is discharged from the separation cup to the packaging bag along the pipeline. When the liquid in the pipeline flows to the packaging bag, the air in the packaging bag is compressed, which makes it difficult for the liquid to flow in, and the liquid in the pipeline is continuously reduced, and the space reduced forms a vacuum, so that the pressure difference condition for the liquid to flow to the packaging bag cannot be formed.

[0003] In addition, during the liquid inlet process, a high negative pressure is formed above the piston during the downward movement of the piston, which causes the liquid in the pipeline to be pressed and instantly rush into the centrifugal cup and the filter, causing the filter to be blocked by the liquid, and the DPM air pressure sensor connected to the filter cannot sense the air pressure change in the pipeline, so that the pressure value in the consumable pipeline cannot be detected. SUMMARY

[0004] In view of the above problems existing in the prior art, the purpose of the present application is to provide a pressure control pipeline and method.

[0005] The specific technical solutions are as follows:

[0006] A pressure control pipeline is designed, which comprises a washing device, a detection device, a liquid packaging device and a gas control device, the communication pipeline of the liquid packaging device is connected with the liquid inlet of the washing device, and the communication pipeline of the gas control device is connected with the air inlet of the washing device.

[0007] The detection device comprises a filter, a first sensor assembly and a first electromagnetic valve, the electromagnetic valve is connected to the first sensor assembly, and the communication pipeline of the first sensor assembly is connected with the filter.

[0008] Preferably, the liquid packaging device comprises a plurality of spin valves, a peristaltic pump and a plurality of packaging bags, the pipelines connected with the plurality of packaging bags are connected to one end of the hose in the peristaltic pump through a multi-head connector.

[0009] Preferably, the washing device comprises a centrifugal cup and a piston, the piston is arranged in the centrifugal cup, the pipeline connected with the liquid inlet at the top of the washing device is connected with a first connector, the other end of the hose in the peristaltic pump is connected with the first connector, and the first connector is also connected with the pipeline connected with the air inlet of the filter.

[0010] Preferably, the lower air vent of the washing device is connected with the communicating pipeline of the gas control device through a three-way joint, and a second sensor assembly is connected with the communicating pipeline.

[0011] Preferably, the gas control device comprises a diaphragm pump, a second electromagnetic valve and a third electromagnetic valve, and the second electromagnetic valve and the third electromagnetic valve are respectively connected with two connecting ports of the diaphragm pump.

[0012] Preferably, the pipeline communicated with the filter outlet is connected with the air inlet of the first sensor assembly, a connecting column is arranged in the inner cavity of the first sensor assembly, and a hose is connected with the bottom of the connecting column, so as to communicate the gas in the pipeline into the first sensor assembly.

[0013] Preferably, the hose is also connected with the first valve port of the first electromagnetic valve, and the first valve port is in a closed state in normal operation.

[0014] Preferably, the packaging bag is divided into a waste liquid bag, an intermediate bag, a washing bag, a sample bag, a preparation bag and a product bag.

[0015] The pipeline connected with the washing bag and the pipeline connected with the sample bag are controlled to be turned on and off by a first rotary valve, the pipeline connected with the waste liquid bag and the pipeline connected with the intermediate bag are controlled to be turned on and off by a second rotary valve, and the pipeline connected with the preparation bag and the pipeline connected with the product bag are controlled to be turned on and off by a third rotary valve.

[0016] A pressure control method is designed: the rotary valve controls the pipeline in which the packaging bag is arranged to be conducted, and the liquid in the packaging bag passes through the peristaltic pump, and part of the liquid enters the liquid inlet of the washing device, and the other part of the liquid enters the filter pipeline;

[0017] The first electromagnetic valve connected with the first sensor assembly controls the normally closed valve port to be conducted, and the gas at the other end of the normally closed valve port is communicated through the pipeline between the first sensor assembly and the filter, so as to squeeze the liquid in the filter pipeline back to the liquid inlet of the washing device.

[0018] The liquid entering the washing device is subjected to centrifugal treatment in the centrifugal cup in the washing device.

[0019] The gas control device controls the gas pressure below the piston in the washing device to increase, and the liquid above in the washing device is pressed to be discharged from the liquid inlet.

[0020] After being discharged, part of the liquid enters the packaging bag under the action of the peristaltic pump, and the other part of the liquid cannot enter the packaging bag due to the increased gas pressure in the packaging bag and remains in the pipeline.

[0021] The first electromagnetic valve controls the normally closed valve port to be conducted, and the gas at the other end of the normally closed valve port enters the filter connecting pipeline through the pipeline communicated between the first sensor assembly and the filter, supplements the gas at one end of the liquid remaining in the pipeline, and squeezes the liquid remaining in the pipeline back into the packaging bag.

[0022] Preferably, the first sensor assembly is used to detect the gas pressure in the communicated pipeline and send a signal to control the opening and closing of the first valve port of the first electromagnetic valve.

[0023] The above technical scheme has the following advantages or beneficial effects:

[0024] The present application provides a pressure control pipeline and method. By improving the structure of the detection device and adding an electromagnetic valve and other devices on the sensor assembly, when the pressure in the pipeline above the washing device is low and the residual liquid cannot enter the packaging bag through the peristaltic pump, or during the liquid inlet process, the pressure in the pipeline above the washing device is high and the liquid is instantaneously flushed into the filter pipeline, the first electromagnetic valve is conducted, the gas in the pipeline is supplemented, and the filter is not blocked. The pressure condition of the pipeline is accurately detected, the damage of the centrifugal cup and other components is avoided, and the pressure difference of the waste liquid in the pipeline is balanced, so that the liquid can be discharged from the washing device along the pipeline to the liquid packaging device. BRIEF DESCRIPTION OF DRAWINGS

[0025] Figure 1 The structure diagram of the washing pipeline system provided by the present application is shown in the figure.

[0026] Figure 2 The first sensor assembly cross-sectional view of the washing pipeline system provided by the present application is shown in the figure.

[0027] LEGEND:

[0028] Centrifugal cup 1, piston 2, filter 3, first sensor assembly 4, peristaltic pump 5, first joint 6, diaphragm pump 7, waste liquid bag 8, first rotary pressure valve 9, washing device 10, detection device 11, liquid packaging device 12, gas control device 13, first electromagnetic valve 41, connecting column 42, hose 43, second sensor assembly 71, second electromagnetic valve 72, third electromagnetic valve 73, intermediate bag 81, washing bag 82, sample bag 83, preparation bag 84, product bag 85, second rotary pressure valve 91, third rotary pressure valve 92, first valve port 411, second valve port 412. DETAILED DESCRIPTION

[0029] With reference to the drawings of the embodiments of the present application, the technical solutions in the embodiments of the present application will be clearly and completely described. Obviously, the described embodiments are only a part of the embodiments of the present application, rather than all the embodiments of the present application. Based on the embodiments of the present application, all the other embodiments obtained by a person of ordinary skill in the art without creative work fall within the scope of the present application.

[0030] It should be noted that the embodiments in the present application and the features in the embodiments can be combined with each other without conflict.

[0031] With reference to the drawings of the embodiments of the present application, the technical solutions in the embodiments of the present application will be clearly and completely described. Obviously, the described embodiments are only a part of the embodiments of the present application, rather than all the embodiments of the present application. Based on the embodiments of the present application, all the other embodiments obtained by a person of ordinary skill in the art without creative work fall within the scope of the present application. Figure 1 A pressure control pipeline, comprising: a washing device 10, a detection device 11, a liquid packaging device 12 and a gas control device 13, a communication pipeline of the liquid packaging device 12 is connected with a liquid inlet of the washing device 10, and a communication pipeline of the gas control device 13 is connected with a gas inlet of the washing device 10.

[0032] The detection device 11 comprises: a filter 3, a first sensor assembly 4 and a first electromagnetic valve 41, the first electromagnetic valve 41 is connected with the first sensor assembly 4, and a communication pipeline of the first sensor assembly 4 is connected with the filter 3; a communication pipeline of the filter 3 is communicated with a pipeline connected with the liquid inlet of the washing device 10.

[0033] The gas control device 13 controls the increase of the gas pressure below the washing device 10, and the liquid above the washing device 10 is pressed to be discharged from the liquid inlet to the liquid packaging device 12 along the pipeline, so that the gas in the pipeline is extruded, the first sensor assembly 4 detects the increase of the gas pressure in the communicated pipeline, sends a signal to the first electromagnetic valve 41, and a normally closed valve port in the first electromagnetic valve 41 is conducted, so that the gas at the other end of the normally closed valve port is supplemented to the pipeline, and the liquid in the pipeline is completely discharged to the liquid packaging bag.

[0034] The liquid packaging device 12 comprises a plurality of rotary pressure valves, a peristaltic pump 5 and a plurality of packaging bags, and the pipelines communicated with the plurality of packaging bags are connected to one end of a hose in the peristaltic pump 5 through a multi-head connector.

[0035] The peristaltic pump 5 serves as a power source, can pump the liquid in the sample bag into the centrifugal cup 1 installed on the high-speed rotating washing device 10, and when the centrifugal cup 1 rotates at high speed, the cells in the cell liquid sample are separated from the liquid according to the principle of density gradient centrifugation in the centrifugal cup 1; the peristaltic pump 5 can also return the processed product to the product bag 85, or transport the waste liquid generated in the washing process of the washing device 10 to the waste liquid bag 8.

[0036] The washing device 10 comprises a centrifugal cup 1 and a piston 2 arranged in the centrifugal cup 1, a pipe connected to a first joint 6 in communication with an upper liquid inlet of the washing device 10, the first joint 6 being connected to the other end of a hose in the peristaltic pump 5, and the first joint 6 being further connected to a pipe in communication with an air inlet of the filter 3, and gas being present in the pipe in communication with the filter 3.

[0037] When the liquid between the washing device 10 and the peristaltic pump 5 is excessive, the gas in the pipe in communication with the filter 4 is squeezed, and the first sensor assembly 4 feeds back an increase in the pressure value;

[0038] The first valve port 411 of the electromagnetic valve 41 is opened, the first valve port 411 and the second valve port 412 are in communication, the gas in the pipe in communication with the filter 3 is replenished, and the filter is prevented from being blocked by the liquid instantaneously rushing into the pipeline due to excessive pressure difference;

[0039] When the liquid between the washing device 10 and the peristaltic pump 5 is reduced, a vacuum section is formed, the gas in the pipe in communication with the filter 3 enters the pipe in communication between the washing device 10 and the peristaltic pump 5 under the action of the pressure difference, the air pressure in the pipe in communication with the filter 3 is reduced, the first sensor assembly 4 feeds back a decrease in the pressure value, and the system determines that the liquid in the centrifugal cup 1 is reduced;

[0040] The first sensor assembly 4 detects different pressure values, thereby detecting the pressure value of the gas above the washing device 10 and in the consumable pipeline.

[0041] The gas control device 13 is connected to the washing device 10 through a pipe and acts on the gas below the piston 2.

[0042] A three-way joint is connected to the pipe in communication between the washing device 10 and the gas control device 13, and a second sensor assembly 71 is arranged on the three-way joint to detect the air pressure of the air inlet of the washing device 10.

[0043] The gas control device 13 comprises a diaphragm pump 7, a second electromagnetic valve 72 and a third electromagnetic valve 73, the second electromagnetic valve 72 and the third electromagnetic valve 73 being respectively connected to two connection ports of the diaphragm pump 7, and the pressure of the air inlet of the washing device 10 being controlled by rotation of the diaphragm pump 7 and on-off control of the second electromagnetic valve 72 and the third electromagnetic valve 73.

[0044] The pipe in communication with one end of the filter 3 is connected to an air inlet of the first sensor assembly 4, a connecting column 42 is arranged in an inner cavity of the first sensor assembly 4, and a hose 43 is connected to a bottom of the connecting column 42 to connect the gas in the pipeline to the sensor.

[0045] The hose 43 is also connected to the first valve port 411 of the first electromagnetic valve 41, which is normally closed, and the second valve port 412 is always open. The second sensor assembly 42 is connected to the second valve port 412 of the first electromagnetic valve 41.

[0046] The packaging bags are divided into waste liquid bags 8, intermediate bags 81, washing bags 82, sample bags 83, preparation bags 84 and product bags 85.

[0047] The pipelines connected to the washing bags 82 and the sample bags 83 are controlled by the first rotary valve 9, the pipelines connected to the waste liquid bags 8 and the intermediate bags 81 are controlled by the second rotary valve 91, and the pipelines connected to the preparation bags 84 and the product bags 85 are controlled by the third rotary valve 92.

[0048] A pressure control method: the rotary valve controls the pipeline where the packaging bag is located to be open, and the liquid in the packaging bag passes through the peristaltic pump 5. At this time, the liquid pressure in the pipeline between the washing device 10 and the peristaltic pump 5 is high, causing the liquid entering the washing device 10 to also instantaneously rush into the pipeline of the filter 3, resulting in part of the liquid entering the liquid inlet of the washing device 10 and part of the liquid entering the pipeline of the filter 3.

[0049] The first electromagnetic valve 41 connected to the first sensor assembly 4 controls the normally closed valve port to be open, and the gas at the other end of the normally closed valve port passes through the pipeline connected between the first sensor assembly 4 and the filter 3 to push the liquid in the pipeline of the filter 3 back to the liquid inlet of the washing device 10.

[0050] The liquid entering the washing device 10 is subjected to centrifugal treatment in the centrifugal cup 1 in the washing device 10.

[0051] The gas control device 13 controls the increase of the gas pressure below the piston 2 in the washing device 10, and the liquid above in the washing device 10 is pressed out of the liquid inlet.

[0052] After being discharged, part of the liquid enters the packaging bag under the action of the peristaltic pump 5, and the other part of the liquid is affected by the increased gas pressure in the packaging bag. At the same time, the liquid in the pipeline decreases, resulting in a vacuum section between the washing device 10 and the packaging bag, and the gas pressure at both ends of the residual waste liquid in the pipeline is unbalanced, which cannot enter the packaging bag and remains in the pipeline.

[0053] The first electromagnetic valve 41 controls the normally closed valve port to be open, and the gas at the other end of the normally closed valve port passes through the pipeline connected between the first sensor assembly 4 and the filter 3 to enter the pipeline connected to the filter 3, and to supplement the gas at one end of the liquid remaining in the pipeline and push the liquid remaining in the pipeline back to the packaging bag.

[0054] When the first sensor assembly 4 detects the pipeline pressure imbalance, the first valve port 411 of the first electromagnetic valve 41 opens, at this time the first valve port 411 is communicated with the second valve port 412, the gas guided by the second valve port 412 is supplemented into the pipeline through the first valve port 411, the gas in the pipeline communicated by the first sensor assembly 4 is supplemented, so that the gas pressure difference of the liquid at both ends of the pipeline meets the condition of the waste liquid entering the packaging bag.

[0055] The air detector can be installed on the pipeline communicated with the peristaltic pump 5 and the washing device 10.

[0056] Working principle: refer to Figures 1-2 The liquid in the pipeline above the washing device 10 enters the packaging bag under the action of the peristaltic pump 5, so that the gas volume in the packaging bag becomes smaller, the gas pressure increases, and the remaining liquid cannot enter the packaging bag due to the influence of the gas pressure, at this time the pressure in the pipeline above the washing device 10 is relatively low, or during the liquid entering process of the washing device 10, the pressure in the pipeline above the washing device 10 is relatively high, so that the liquid is instantaneously flushed into the filter pipeline, for the above two cases, the first valve port 411 of the first electromagnetic valve 41 opens, at this time the first valve port 411 is communicated with the second valve port 412, the gas in the pipeline communicated by the first sensor assembly 4 is supplemented, the pressure difference of the liquid in the pipeline is balanced, so that the liquid is discharged from the washing device 10 along the pipeline to the liquid packaging device 12.

[0057] The above only describes the preferred embodiments of the present application, and does not limit the implementation and protection scope of the present application, for those skilled in the art, it should be realized that the schemes obtained by equivalent replacement and obvious changes according to the content of the present application can be included in the protection scope of the present application.

Claims

1. A control method of a pressure control line, characterized by, The control pipeline comprises a washing device, a detection device, a liquid packaging device and a gas control device, a communication pipeline of the liquid packaging device is connected with a liquid inlet of the washing device, and a communication pipeline of the gas control device is connected with a gas inlet of the washing device; The detection device comprises a filter, a first sensor assembly and a first electromagnetic valve, the first electromagnetic valve is connected to the first sensor assembly, and a communication pipeline of the first sensor assembly is connected with the filter; a communication pipeline of the filter is communicated with a pipeline connected with the liquid inlet of the washing device; The liquid packaging device comprises a plurality of spin valves, a peristaltic pump and a plurality of packaging bags, pipelines connected with the plurality of packaging bags are connected to one end of a hose in the peristaltic pump through a multi-head connector; The washing device comprises a centrifugal cup and a piston, the piston is arranged in the centrifugal cup, a pipeline connected with an upper liquid inlet of the washing device is connected with a first connector, the first connector is connected to the other end of the hose in the peristaltic pump, and the first connector is also connected with a pipeline connected with an air inlet of the filter; A second sensor assembly is connected to the communication pipeline of the gas control device through a three-way connector at a lower gas inlet of the washing device; A communication pipeline at one end of the filter is connected to an air inlet of the first sensor assembly, a connecting column is arranged in an inner cavity of the first sensor assembly, and a hose is connected to the bottom of the connecting column, so as to connect the gas in the pipeline to the first sensor assembly; The hose is also connected to a first valve port of the first electromagnetic valve, the first valve port is in a closed state when normally working, compressed air is stored in a cavity between a second valve port and the first sensor assembly, and the second valve port is always in a conducting state; The control method comprises: The spin valve controls the pipeline where the packaging bag is located to be conducted, the liquid in the packaging bag passes through the peristaltic pump, and part of the liquid enters the liquid inlet of the washing device, and the other part enters the pipeline in the filter; The first electromagnetic valve connected with the first sensor assembly controls the first valve port to be conducted, and the gas at the other end of the first valve port passes through the pipeline connected between the first sensor assembly and the filter, so as to squeeze the liquid in the pipeline in the filter back to the liquid inlet of the washing device; The liquid entering the washing device is subjected to centrifugal treatment in the centrifugal cup in the washing device; The gas control device controls the gas pressure below the piston in the washing device to increase, and the liquid above in the washing device is pressed to be discharged from the liquid inlet; After being discharged, part of the liquid enters the packaging bag under the action of the peristaltic pump, and the other part of the liquid cannot enter the packaging bag due to the increased gas pressure in the packaging bag and remains in the pipeline; The first electromagnetic valve controls the first valve port to be conducted, and the gas at the other end of the first valve port enters the pipeline connected with the filter through the pipeline connected between the first sensor assembly and the filter, so as to supplement the gas at one end of the liquid remaining in the pipeline and squeeze the liquid remaining in the pipeline back to the packaging bag.

2. The control method of the pressure control line according to claim 1, characterized by: The gas control device comprises a diaphragm pump, a second electromagnetic valve and a third electromagnetic valve, the second electromagnetic valve and the third electromagnetic valve are respectively connected to two connection ports of the diaphragm pump.

3. The control method of the pressure control line according to claim 1, characterized by: The packaging bag is divided into a waste liquid bag, an intermediate bag, a washing bag, a sample bag, a preparation bag and a product bag, and the spin valve includes a first spin valve, a second spin valve and a third spin valve. The pipeline connected with the washing bag and the pipeline connected with the sample bag are controlled by the first spin valve, the pipeline connected with the waste liquid bag and the pipeline connected with the intermediate bag are controlled by the second spin valve, and the pipeline connected with the preparation bag and the pipeline connected with the product bag are controlled by the third spin valve.

4. The control method of the pressure control line according to claim 1, characterized by: The first sensor assembly is used for detecting the air pressure in the pipeline communicated between the first sensor assembly and the filter and sending a signal to control the opening and closing of the first valve port of the first electromagnetic valve.

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