A light source calibration method for an IVD testing instrument and the IVD testing instrument itself.

Abstract

A light source calibration method and an IVD testing instrument are disclosed, relating to the field of optoelectronic device testing technology. The method includes: acquiring the photoelectric value of the air medium when the IVD testing instrument is powered on (initial air photoelectric value); acquiring the photoelectric value of the air medium when the IVD testing instrument is placed with a sample (sample air photoelectric value); acquiring the photoelectric value of the sample medium of the sample (sample sample); determining a proportionality coefficient based on the initial air photoelectric value and the sample air photoelectric value; determining the sample photoelectric value based on the proportionality coefficient and the sample medium photoelectric value; and determining the absorbance based on the sample photoelectric value. This application utilizes the property that the light transmittance of the air medium inside the IVD testing instrument remains constant. The proportionality coefficient obtained by calculating the sample air photoelectric value and the original air photoelectric value at startup more completely reflects the changes in the light source. Furthermore, by using this proportionality coefficient and the sample medium photoelectric value for calculation, the influence of changes in the light source can be eliminated, thereby eliminating inter-instrument differences.

Description

Technical Field

[0001] This invention relates to the field of optoelectronic device testing technology, specifically to a light source calibration method and an IVD testing instrument. Background Technology

[0002] Currently, most IVD (In Vitro Diagnostic Products) testing instruments on the market use halogen bulbs, which offer relatively stable light sources. However, halogen bulbs generate a lot of heat, have limited lifespan, are expensive, and require a large heat dissipation structure. Therefore, even with this highly stable light source, significant inter-sample variability (inter-sample variability refers to the difference in data obtained from single or multiple average measurements of the same sample by two or more identical instruments under the same environment, operated by the same person, using the same procedures and techniques) cannot be avoided due to external temperature and circuit factors. Subsequent market developments have addressed this by adjusting the gain of the photoelectric conversion circuit, which can only be adjusted at the start of the test and not during the test itself. This method becomes inadequate when a test is lengthy. Summary of the Invention

[0003] The main technical problem solved by this invention is that the inter-instrument difference in IVD testing instruments is large during the testing process.

[0004] According to a first aspect, one embodiment provides a light source calibration method for an IVD (In Vitro Diagnostics) instrument, comprising:

[0005] Obtain the photoelectric value of the air medium when the IVD testing instrument is powered on, which is the initial air photoelectric value;

[0006] When the IVD testing instrument is placed into the sample to be tested, the photoelectric value of the air medium is obtained as the photoelectric value of the sample air, and the photoelectric value of the sample medium of the sample to be tested is obtained.

[0007] A proportionality coefficient is determined based on the initial air photoelectric value and the sample air photoelectric value; the sample photoelectric value is determined based on the proportionality coefficient and the sample medium photoelectric value; and the absorbance is determined based on the sample photoelectric value.

[0008] In one embodiment, determining the proportionality coefficient based on the initial air photoelectric value and the sample air photoelectric value includes:

[0009] The initial air photoelectric value is divided by the sample air photoelectric value to determine the scaling factor.

[0010] In one embodiment, determining the photoelectric value of the sample based on the proportionality coefficient and the photoelectric value of the sample medium includes:

[0011] The proportionality coefficient is multiplied by the photoelectric value of the sample medium to determine the photoelectric value of the sample.

[0012] In one embodiment, determining the absorbance based on the photoelectric value of the sample includes:

[0013] The absorbance is calculated using the following formula:

[0014]

[0015] Where A is absorbance, and AD 空气 AD represents the photoelectric value of the sample air. 暗 AD is the photoelectric value of the sample air when the sample is placed in the IVD testing instrument and the light source is turned off. 溶液 This is the photoelectric value of the sample.

[0016] According to a second aspect, one embodiment provides an IVD (In Vitro Diagnostics) instrument, comprising:

[0017] An optical module is used to acquire the photoelectric value of the air medium when the IVD testing instrument is powered on, which is the initial air photoelectric value; the optical module is also used to acquire the photoelectric value of the air medium when the IVD testing instrument is placed in the sample, which is the sample air photoelectric value, and to acquire the photoelectric value of the sample medium of the sample to be tested;

[0018] The processor is configured to determine a scaling factor based on the initial air photoelectric value and the sample air photoelectric value, determine a sample photoelectric value based on the scaling factor and the sample medium photoelectric value, and determine absorbance based on the sample photoelectric value.

[0019] In one embodiment, determining the proportionality coefficient based on the initial air photoelectric value and the sample air photoelectric value includes:

[0020] The initial air photoelectric value is divided by the sample air photoelectric value to determine the scaling factor.

[0021] In one embodiment, determining the photoelectric value of the sample based on the proportionality coefficient and the photoelectric value of the sample medium includes:

[0022] The proportionality coefficient is multiplied by the photoelectric value of the sample medium to determine the photoelectric value of the sample.

[0023] In one embodiment, determining the absorbance based on the photoelectric value of the sample includes:

[0024] The absorbance is calculated using the following formula:

[0025]

[0026] Where A is absorbance, and AD 空气 AD represents the photoelectric value of the sample air. 暗 AD is the photoelectric value of the sample air when the sample is placed in the IVD testing instrument and the light source is turned off. 溶液 This is the photoelectric value of the sample.

[0027] In one embodiment, the optical module includes a light source module, which includes at least one of a laser or an LED bead.

[0028] According to a third aspect, one embodiment provides a computer-readable storage medium storing a program that can be executed by a processor to implement the above-described method.

[0029] A light source calibration method for an IVD testing instrument, an IVD testing instrument, and a computer-readable storage medium are disclosed according to the above embodiments. The method involves acquiring the air photoelectric value when the IVD testing instrument is powered on, and also acquiring the air photoelectric value when the sample to be tested is placed in the IVD testing instrument. A proportionality coefficient is obtained by proportionally calculating the air photoelectric value when the instrument is powered on and the air photoelectric value when the sample is placed. This proportionality coefficient is then used to calculate the photoelectric value of the sample medium to obtain the final sample photoelectric value. The absorbance is then calculated using the sample photoelectric value. This application utilizes the property that the light transmittance of the air medium inside the IVD testing instrument remains constant. The proportionality coefficient obtained by calculating the air photoelectric value of the sample after it is placed and the original air photoelectric value when the instrument is powered on more completely reflects the change in the light source. Using this proportionality coefficient and the photoelectric value of the sample medium, the influence of the change in the light source can be eliminated, thereby eliminating inter-instrument differences. Attached Figure Description

[0030] Figure 1 This is one embodiment of a light source calibration method for an IVD testing instrument;

[0031] Figure 2 This is a schematic diagram of the optical module of an IVD detection instrument in one embodiment;

[0032] Figure 3 This is a schematic diagram of an IVD testing instrument in one embodiment. Figure 1 ;

[0033] Figure 4 This is a schematic diagram of an IVD testing instrument in one embodiment. Figure 2 . Detailed Implementation

[0034] The present invention will now be described in further detail with reference to specific embodiments and accompanying drawings. Similar elements in different embodiments are referred to by associated similar element reference numerals. In the following embodiments, many details are described to facilitate a better understanding of this application. However, those skilled in the art will readily recognize that some features may be omitted in different situations, or may be replaced by other elements, materials, or methods. In some cases, certain operations related to this application are not shown or described in the specification. This is to avoid obscuring the core parts of this application with excessive description. For those skilled in the art, detailed description of these related operations is not necessary; they can fully understand the related operations based on the description in the specification and general technical knowledge in the art.

[0035] Furthermore, the features, operations, or characteristics described in the specification can be combined in any suitable manner to form various embodiments. At the same time, the steps or actions in the method description can be rearranged or adjusted in a manner obvious to those skilled in the art. Therefore, the various orders in the specification and drawings are only for the clear description of a particular embodiment and do not imply a necessary order, unless otherwise stated that a particular order must be followed.

[0036] The serial numbers assigned to components in this document, such as "first" and "second," are used only to distinguish the described objects and have no sequential or technical meaning. The terms "connection" and "linkage" used in this application, unless otherwise specified, include both direct and indirect connections (linkages).

[0037] Please refer to Figure 1 This application provides a light source calibration method for an IVD testing instrument in some embodiments, including the following steps.

[0038] Step S110: Obtain the photoelectric value of the air medium when the IVD testing instrument is powered on, which is the initial air photoelectric value.

[0039] In some embodiments, please refer to Figure 2 This is a schematic diagram of the optical path of an IVD (In Vitro Diagnostics) instrument. An LED driver turns the light source on or off, and a beam splitter separates the light into two paths. The first path acquires the photoelectric value of the air, and the second path acquires the photoelectric value of the sample medium. When the IVD instrument is powered on and no sample is placed in it, there is no sample in the second optical path. The LED driver turns on the light source, and the first optical path acquires the photoelectric value of the air medium at this time. A diode converts the light signal into an electrical signal, which is the initial photoelectric value of the air.

[0040] Step S120: Obtain the photoelectric value of the air medium when the IVD testing instrument is placed in the sample to be tested, and obtain the photoelectric value of the sample medium of the sample to be tested.

[0041] In some embodiments, the sample to be tested is placed manually or automatically by the IVD testing instrument at the position illuminated by the second optical path. At this time, the light source is also turned on using an LED driver. The photoelectric value of the air medium after the sample is placed is detected using the first optical path. Compared to when the IVD testing instrument is powered on, the light source may have deviated due to temperature, voltage, or current effects. Therefore, the photoelectric value of the air medium after the sample is placed is obtained using the first optical path, and the optical signal is converted into an electrical signal using a diode, which is the photoelectric value of the sample air. The photoelectric value of the sample is obtained using the second optical path, and the optical signal is also converted into an electrical signal using a diode, which is the photoelectric value of the sample medium.

[0042] Step S130: Determine the proportionality coefficient based on the initial air photoelectric value and the sample air photoelectric value, determine the sample photoelectric value based on the proportionality coefficient and the sample medium photoelectric value, and determine the absorbance based on the sample photoelectric value.

[0043] In some embodiments, a proportionality coefficient can be calculated by dividing the initial air photoelectric value obtained in step S110 by the sample air photoelectric value obtained in step S120. The sample photoelectric value can be calculated by multiplying the proportionality coefficient by the sample medium photoelectric value obtained in step S120. Finally, the absorbance is calculated using the following formula:

[0044]

[0045] Where A is absorbance, and AD 空气 AD represents the photoelectric value of the sample air. 暗 AD is the photoelectric value of the sample air when the sample is placed in the IVD testing instrument and the light source is turned off. 溶液 This is the photoelectric value of the sample.

[0046] In some embodiments, the initial air photoelectric value obtained by the IVD testing instrument when it is initially powered on is the most accurate. When the sample to be tested is placed in the instrument, the light source may have deviated due to the influence of temperature, current or voltage. However, since the light transmittance of the air medium cannot change, the proportionality coefficient obtained by dividing the initial air photoelectric value by the sample air photoelectric value can reflect the current change of the light source in real time. The photoelectric value calculated by this proportionality coefficient can eliminate the influence of the change of the light source.

[0047] Please refer to Figure 3 In some embodiments of this application, an IVD inspection instrument 300 is also provided, including an optical module 310 and a processor 320, which will be described in detail below.

[0048] Please refer to Figure 2 The optical module 310 includes an LED driver 311, a beam splitter 312, a first detection unit 313, and a second detection unit 314. Under the action of the beam splitter 312, the optical path is divided into a first optical path and a second optical path. The first detection unit 313 and the second detection unit 314 are used to convert optical signals into electrical signals. In some embodiments, the first detection unit 313 and the second detection unit 314 are photodiodes.

[0049] In some embodiments, the LED driver 311 is used to turn the light source on or off. When the IDV testing instrument 300 is powered on, the LED driver 311 turns on the light source, uses the first optical path to acquire the photoelectric value of the air medium, and uses the first detection unit 313 to convert the light signal into an electrical signal to obtain the initial air photoelectric value. While keeping the LED driver 311 on, when a sample to be tested is manually or automatically placed in the IDV testing instrument 300 (i.e., manually or automatically placed within the illumination range of the second optical path), the first optical path acquires the air photoelectric value after the sample is placed, and the first detection unit 313 converts the light signal into an electrical signal to obtain the sample air photoelectric value. The second optical path acquires the photoelectric value of the sample to be tested, and the second detection unit 314 converts the light signal into an electrical signal to obtain the sample medium photoelectric value.

[0050] The processor 320 is used to determine a scaling factor based on the initial air photoelectric value and the sample air photoelectric value, then determine the sample photoelectric value based on the scaling factor and the sample medium photoelectric value, and finally determine the absorbance based on the sample photoelectric value.

[0051] In some embodiments, the processor 320 calculates a scaling factor by dividing the initial air photoelectric value by the sample air photoelectric value. Multiplying the scaling factor by the sample medium photoelectric value yields the sample photoelectric value, and finally, the absorbance is calculated using the following formula:

[0052]

[0053] Where A is absorbance, and AD 空气 AD represents the photoelectric value of the sample air. 暗 AD is the photoelectric value of the sample air when the sample is placed in the IVD testing instrument and the light source is turned off. 溶液 This is the photoelectric value of the sample.

[0054] In some embodiments, the initial air photoelectric value acquired by the IVD testing instrument 300 upon initial power-on is the most accurate. When the IVD testing instrument 300 is placed with the sample to be tested, the light source may have deviated due to the influence of temperature, current, or voltage. However, since the light transmittance of the air medium cannot change, a proportionality coefficient obtained by dividing the initial air photoelectric value by the sample air photoelectric value can reflect the current change in the light source in real time. Furthermore, the photoelectric value calculated using this proportionality coefficient can eliminate the influence of the light source change.

[0055] Please refer to Figure 4 In some embodiments, the optical module 310 further includes a light source module 315, which includes at least one laser or LED light source. Using small and inexpensive light sources such as lasers or LED light sources is particularly important in some POCT (point-of-care testing) applications, as it can significantly reduce costs.

[0056] This application provides a light source calibration method and an IVD testing instrument. Utilizing the constant light transmittance of the air medium inside the IVD testing instrument, a proportionality coefficient is obtained by proportionally calculating the photoelectric value of the air medium without a sample and the photoelectric value of the air medium with a sample. This coefficient is then used to calculate the sample photoelectric value, which is finally used to determine the absorbance. Because the air medium's light transmittance remains constant, the calculated proportionality coefficient more completely reflects changes in the light source, thus eliminating the influence of light source variations on the absorbance. This application eliminates the need for complex circuit design, significantly reducing the cost of hardware drive circuits and overcoming test discrepancies caused by light source instability. Even with continuous changes in temperature, voltage, or current, the IVD instrument can still obtain accurate absorbance.

[0057] Those skilled in the art will understand that all or part of the functions of the various methods in the above embodiments can be implemented by hardware or by computer programs. When all or part of the functions in the above embodiments are implemented by computer programs, the program can be stored in a computer-readable storage medium, which may include: read-only memory, random access memory, disk, optical disk, hard disk, etc., and the program is executed by a computer to achieve the above functions. For example, the program can be stored in the memory of a device, and when the program in the memory is executed by the processor, all or part of the above functions can be achieved. In addition, when all or part of the functions in the above embodiments are implemented by computer programs, the program can also be stored in a server, another computer, disk, optical disk, flash drive, or external hard drive, etc., and can be downloaded or copied to the memory of a local device, or the system of the local device can be updated. When the program in the memory is executed by the processor, all or part of the functions in the above embodiments can be achieved.

[0058] The above examples illustrate the present invention only to aid in understanding it and are not intended to limit the scope of the invention. Those skilled in the art can make various simple deductions, modifications, or substitutions based on the principles of this invention.

Claims

1. A method for calibrating the light source of an IVD (In Vitro Diagnostics) testing instrument, characterized in that, include: The photoelectric value of the air medium is obtained when the IVD testing instrument is powered on, and the initial air photoelectric value is obtained when the IVD testing instrument is powered on and no sample to be tested is placed in it. When the IVD testing instrument is placed into the sample to be tested, the photoelectric value of the air medium is obtained as the photoelectric value of the sample air, and the photoelectric value of the sample medium of the sample to be tested is obtained. A proportionality coefficient is determined based on the initial air photoelectric value and the sample air photoelectric value; the sample photoelectric value is determined based on the proportionality coefficient and the sample medium photoelectric value; and the absorbance is determined based on the sample photoelectric value. The absorbance is calculated using the following formula: Where A is absorbance, and AD 空气 The sample air photoelectric value, AD 暗 AD is the photoelectric value of the sample air when the sample is placed in the IVD testing instrument and the light source is turned off. 溶液 This is the photoelectric value of the sample.

2. The light source calibration method for the IVD testing instrument as described in claim 1, characterized in that, The step of determining the proportionality coefficient based on the initial air photoelectric value and the sample air photoelectric value includes: The initial air photoelectric value is divided by the sample air photoelectric value to determine the scaling factor.

3. The light source calibration method for the IVD testing instrument as described in claim 1, characterized in that, The step of determining the photoelectric value of the sample based on the proportionality coefficient and the photoelectric value of the sample medium includes: The proportionality coefficient is multiplied by the photoelectric value of the sample medium to determine the photoelectric value of the sample.

4. An IVD (In Vitro Diagnostics) testing instrument, characterized in that, include: An optical module is used to acquire the photoelectric value of the air medium when the IVD testing instrument is powered on, which is the initial air photoelectric value. The optical module is also used to acquire the photoelectric value of the air medium when the IVD testing instrument is placed with a sample to be tested, which is the sample air photoelectric value, and to acquire the photoelectric value of the sample medium of the sample to be tested. The initial air photoelectric value is the photoelectric value of the air medium when the IVD testing instrument is powered on but no sample to be tested is placed in it. The processor is configured to determine a scaling factor based on the initial air photoelectric value and the sample air photoelectric value, determine a sample photoelectric value based on the scaling factor and the sample medium photoelectric value, and determine absorbance based on the sample photoelectric value; The absorbance is calculated using the following formula: Where A is absorbance, and AD 空气 The sample air photoelectric value, AD 暗 AD is the photoelectric value of the sample air when the sample is placed in the IVD testing instrument and the light source is turned off. 溶液 This is the photoelectric value of the sample.

5. The IVD testing instrument as described in claim 4, characterized in that, The step of determining the proportionality coefficient based on the initial air photoelectric value and the sample air photoelectric value includes: The initial air photoelectric value is divided by the sample air photoelectric value to determine the scaling factor.

6. The IVD testing instrument as described in claim 4, characterized in that, The step of determining the photoelectric value of the sample based on the proportionality coefficient and the photoelectric value of the sample medium includes: The proportionality coefficient is multiplied by the photoelectric value of the sample medium to determine the photoelectric value of the sample.

7. The IVD testing instrument as described in claim 4, characterized in that, The optical module includes a light source module, which includes at least one of a laser or an LED bead.

8. A computer-readable storage medium, characterized in that, The medium stores a program that can be executed by a processor to implement the method as described in any one of claims 1-3.

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