A composition for controlling Spodoptera frugiperda

Abstract

The present invention discloses a composition for controlling Spodoptera frugiperda, belonging to the technical field of chemical pest control. The composition for controlling Spodoptera frugiperda comprises the following components in mass percentage: 10-80% of active ingredient and 20-90% of auxiliary material; the active ingredient includes chlorantraniliprole, marine cyanobacteria extract and Litsea cubeba extract. The medicament composition for controlling Spodoptera frugiperda of the present invention has a good control effect on Spodoptera frugiperda and a stable control efficacy, and can slow down the drug resistance of Spodoptera frugiperda to the medicament and prolong the service life of the medicament.

Description

Technical Field

[0001] The present invention relates to the technical field of chemical pest control, and particularly relates to a composition for controlling Spodoptera frugiperda. Background Art

[0002] Spodoptera frugiperda (J.E. Smith) belongs to the genus Spodoptera of the family Noctuidae in the order Lepidoptera. It is one of the major migratory agricultural pests globally warned by the Food and Agriculture Organization of the United Nations. It has the characteristics of a wide host range, strong reproductive ability, fast migration and diffusion, severe damage degree, and great difficulty in prevention and control.

[0003] At present, the control measures for Spodoptera frugiperda mainly include: (1) agricultural control; (2) physical and chemical trapping and control; (3) chemical control; (4) biological control. At the present stage, the control of Spodoptera frugiperda still mainly relies on chemical pesticides. In order to obtain better control effects, the dosage of chemical drugs has been continuously increasing, resulting in a gradual increase in the resistance of Spodoptera frugiperda to chemical drugs. Moreover, the research of new chemical drugs is relatively difficult. Therefore, delaying the resistance of Spodoptera frugiperda to existing chemical drugs and reducing the dosage of existing chemical drugs have become urgent problems to be solved by those skilled in the art. Summary of the Invention

[0004] The purpose of the present invention is to provide a composition for controlling Spodoptera frugiperda to solve the problems existing in the above-mentioned prior art.

[0005] To achieve the above purpose, the present invention provides the following solutions:

[0006] One of the technical solutions of the present invention: A composition for controlling Spodoptera frugiperda, comprising the following components in mass percentage: 15 - 40% of active ingredients and 60 - 85% of auxiliary materials;

[0007] The active ingredients include chlorantraniliprole, marine cyanobacterium extract, and Litsea cubeba extract.

[0008] Further, the mass ratio of chlorantraniliprole, marine cyanobacterium extract, and Litsea cubeba extract is (1 - 10):(1 - 5):(1 - 2).

[0009] Further, the preparation method of the marine cyanobacterium extract specifically includes:

[0010] (1) Activate the marine cyanobacterium and inoculate it in a liquid medium for culture to obtain a marine cyanobacterium fermentation broth;

[0011] (2) Centrifuge the fermentation broth of marine cyanobacteria to obtain marine cyanobacterial cells. Add water to the marine cyanobacterial cells, ultrasonically disrupt them, and extract with ethyl acetate 2 - 3 times, then extract with n-butanol 2 - 3 times. Combine the extracts to obtain the marine cyanobacterial extract.

[0012] The marine cyanobacterial extract is rich in cyclic peptide compounds. Such compounds can quickly enter the cells of pests, affect the cell permeability of pests, cause the contents inside the cells to exude, resulting in limited cell function, slowed metabolism, reduced migratory ability, and decreased activity range. The chlorantraniliprole contained in the crops bitten by pests within a small range enters the pests' bodies, activates the ryanodine receptors of pests, stimulates the release of calcium ions stored in the smooth muscles of pests, and causes the pests to stop feeding, convulse, vomit, become unconscious, have muscle contraction and rigidity, and finally die.

[0013] The Litsea cubeba extract enters the pests' bodies, reduces the content of acetylcholinesterase in pests, and increases the content of superoxide dismutase, resulting in neurotoxicity to pests.

[0014] Furthermore, in step (1), the culture temperature is 28 ± 2 °C, the culture time is 10 - 15 d, and the culture rotation speed is 100 - 150 r / min; in step (2), the centrifugation speed is 3000 - 3500 r / min, and the time is 10 - 12 min; the power of ultrasonic disruption is 200 - 300 W, and the time is 5 - 8 min.

[0015] Furthermore, the preparation method of the Litsea cubeba extract specifically includes: drying the Litsea cubeba stems and leaves and grinding them into powder, adding an ethanol solution for heating and reflux extraction, and drying the extract after rotary evaporation to obtain the Litsea cubeba extract.

[0016] Furthermore, the volume fraction of the ethanol solution is 75 - 85%; the time for heating and reflux extraction is 2 - 3 h.

[0017] Furthermore, the excipients include wetting agents, thickening agents, dispersing agents, defoaming agents, and water.

[0018] Even further, the composition for controlling Spodoptera frugiperda includes the following components in mass percentage: active ingredient 15 - 40%, wetting agent 2 - 6%, thickening agent 1 - 4%, dispersing agent 4 - 8%, defoaming agent 0.1 - 1.5%, and the balance is water.

[0019] Further, the wetting agent includes one or more of fatty alcohol polyoxyethylene ether, sodium dodecylbenzenesulfonate, and sophora powder; the thickening agent includes one or more of hydroxyethyl cellulose, xanthan gum, and polyvinylpyrrolidone; the dispersant includes one or more of sodium lignosulfonate, benzyl methacrylate, and polycarboxylate; the defoaming agent is a silicone defoaming agent.

[0020] The second technical solution of the present invention: A preparation method of the above composition, comprising the following steps: Weigh each raw material by mass percentage, grind and mix evenly to obtain the composition for controlling Spodoptera frugiperda.

[0021] The third technical solution of the present invention: An application of the above composition in controlling Spodoptera frugiperda in crop fields.

[0022] Furthermore, the method of the application is: Spraying the above composition in crop fields, and the dosage of the composition calculated by the active ingredient is 3.0 - 3.5 g / 667m 2 。

[0023] The present invention discloses the following technical effects:

[0024] The components such as chlorantraniliprole, marine cyanobacteria extract, and litsea cubeba extract in the medicament composition for controlling Spodoptera frugiperda of the present invention act together, have a good control effect on Spodoptera frugiperda and the control effect is stable, and can slow down the drug resistance of Spodoptera frugiperda to the medicament and extend the service life of the medicament.

[0025] The composition of the present invention can greatly reduce the amount of pesticides used in the field, effectively reduce environmental pollution and pesticide residues. Detailed Embodiments

[0026] Now, various exemplary embodiments of the present invention will be described in detail. This detailed description should not be considered as a limitation of the present invention, but should be understood as a more detailed description of certain aspects, characteristics, and implementation schemes of the present invention.

[0027] It should be understood that the terms described in the present invention are only for describing specific embodiments and are not used to limit the present invention. Additionally, for the numerical ranges in the present invention, it should be understood that each intermediate value between the upper and lower limits of the range is also specifically disclosed. Each intermediate value within any stated value or stated range, as well as each smaller range between any other stated value or intermediate value within the stated range, is also included in the present invention. The upper and lower limits of these smaller ranges can be independently included or excluded from the range.

[0028] Unless otherwise specified, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention pertains. Although this invention only describes preferred methods and materials, any methods and materials similar or equivalent to those described herein can also be used in the practice or testing of this invention. All documents mentioned in this specification are incorporated by reference to disclose and describe the methods and / or materials related to the said documents. In case of conflict with any incorporated document, the content of this specification shall prevail.

[0029] Without departing from the scope or spirit of this invention, various improvements and changes can be made to the specific embodiments of the specification of this invention, which are obvious to those skilled in the art. Other embodiments obtained from the specification of this invention are obvious to those skilled in the art. The specification and examples of this application are merely exemplary.

[0030] Regarding the use of "comprising", "including", "having", "containing", etc. in this article, they are all open-ended terms, meaning including but not limited to.

[0031] The marine cyanobacteria used in the following examples and comparative examples of this invention were commercially purchased.

[0032] Example 1

[0033] A preparation method of a composition for controlling Spodoptera frugiperda:

[0034] The composition for controlling Spodoptera frugiperda is composed of the following components by mass percentage: chlorantraniliprole 24%, marine cyanobacteria extract 9%, Litsea cubeba extract 3%, wetting agent (sodium dodecylbenzenesulfonate) 3%, thickening agent (xanthan gum) 1%, dispersant (sodium lignosulfonate) 5%, defoaming agent (organosilicon defoamer) 0.5%, and water is added to make up to 100%.

[0035] A preparation method of the marine cyanobacteria extract:

[0036] (1) Inoculate the marine cyanobacteria on a PDA solid slant medium and activate it in an incubator at 28°C for 5 days. Then pick 2 loops and inoculate them into 250 mL of BG11 liquid medium, and culture them for 12 days under the condition that the temperature is about 29°C, and control the shaking speed of the shaker during the culture period to be 120 r / min. After the culture is completed, the marine cyanobacteria fermentation broth is obtained.

[0037] (2) Centrifuge the marine cyanobacteria fermentation broth (at a rotational speed of 3000 r / min for 10 min) to obtain marine cyanobacteria cells; then mix the marine cyanobacteria cells and water at a mass / volume ratio of 1 g:50 mL, and disrupt them under 250 W ultrasound for 6 min to obtain a cell content suspension; extract the cell content suspension 3 times with an equal volume of ethyl acetate and then 3 times with an equal volume of n-butanol, and combine the extracts after rotary evaporation to obtain the marine cyanobacteria extract.

[0038] Preparation method of Litsea cubeba extract: Dry and grind the stems and leaves of Litsea cubeba into powder (100 mesh), add an ethanol solution with a volume fraction of 80% (the mass / volume ratio of the stems and leaves of Litsea cubeba to the ethanol solution is 1 g:20 mL), mix evenly, heat under reflux for 2.5 h, dry the extract after rotary evaporation to obtain the Litsea cubeba extract.

[0039] Preparation method: Weigh each raw material by mass percentage, mix them evenly and put them into a grinder, and grind until the particle size is 5 μm to obtain the composition (suspension agent) for controlling Spodoptera frugiperda.

[0040] Example 2

[0041] A preparation method of a composition for controlling Spodoptera frugiperda:

[0042] The composition for controlling Spodoptera frugiperda is composed of the following components by mass percentage: chlorantraniliprole 5%, marine cyanobacteria extract 5%, Litsea cubeba extract 5%, wetting agent (fatty alcohol polyoxyethylene ether) 2%, thickening agent (hydroxyethyl cellulose) 1%, dispersant (benzyl methacrylate) 4%, defoaming agent (organosilicon defoamer) 0.1%, and add water to make up to 100%.

[0043] Preparation method of marine cyanobacteria extract:

[0044] (1) Inoculate marine cyanobacteria on a PDA solid slant medium, activate them in an incubator at 28 °C for 5 d, then pick 2 loops and inoculate them into 250 mL of BG11 liquid medium, and culture them for 15 d under the condition of about 28 °C, controlling the shaker rotational speed during the culture period to be 100 r / min, and obtain the marine cyanobacteria fermentation broth after the culture ends.

[0045] (2) Centrifuge the marine cyanobacteria fermentation broth (at a rotational speed of 3500 r / min for 12 min) to obtain marine cyanobacteria cells; then mix the marine cyanobacteria cells and water at a mass / volume ratio of 1 g:50 mL, and disrupt them under 200 W ultrasound for 8 min to obtain a cell content suspension; extract the cell content suspension 2 times with an equal volume of ethyl acetate and then 3 times with an equal volume of n-butanol, and combine the extracts after rotary evaporation to obtain the marine cyanobacteria extract.

[0046] Preparation method of Litsea cubeba extract: Dry the stems and leaves of Litsea cubeba and grind them into powder (100 mesh), add ethanol solution with a volume fraction of 75% (the mass / volume ratio of the stems and leaves of Litsea cubeba to the ethanol solution is 1 g:20 mL), mix evenly, heat under reflux for 3 h, evaporate the extract by rotary evaporation and then dry it to obtain Litsea cubeba extract.

[0047] Preparation method: Weigh each raw material by mass percentage, mix them evenly and put them into a grinder, grind until the particle size is 5 μm to obtain a composition (suspension agent) for controlling Spodoptera frugiperda.

[0048] Example 3

[0049] A preparation method of a composition for controlling Spodoptera frugiperda:

[0050] The composition for controlling Spodoptera frugiperda is composed of the following components by mass percentage: Chlorantraniliprole 20%, marine cyanobacterium extract 10%, Litsea cubeba extract 4%, wetting agent (gleditsia sinensis powder) 6%, thickening agent (polyvinylpyrrolidone) 2%, dispersing agent (polycarboxylate) 5%, defoaming agent (organosilicon defoamer) 1%, add water to make up to 100%.

[0051] Preparation method of marine cyanobacterium extract:

[0052] (1) Inoculate marine cyanobacterium on PDA solid slant medium, activate it in an incubator at 28 °C for 5 d, then pick 2 loops and inoculate them in 250 mL BG11 liquid medium, culture them for 10 d under the condition that the temperature is about 30 °C, control the shaking speed of the shaker during the culture period to be 150 r / min, and obtain marine cyanobacterium fermentation broth after the culture ends.

[0053] (2) Centrifuge the marine cyanobacterium fermentation broth (the centrifugation speed is 3200 r / min and the time is 11 min) to obtain marine cyanobacterium cells; then mix the marine cyanobacterium cells and water at a mass / volume ratio of 1 g:50 mL, break them under 300 W ultrasonic waves for 5 min to obtain a cell content suspension; extract the cell content suspension 3 times with an equal volume of ethyl acetate, then extract it 2 times with an equal volume of n-butanol, evaporate by rotary evaporation and combine the extracts to obtain marine cyanobacterium extract.

[0054] Preparation method of Litsea cubeba extract: Dry the stems and leaves of Litsea cubeba and grind them into powder (100 mesh), add ethanol solution with a volume fraction of 85% (the mass / volume ratio of the stems and leaves of Litsea cubeba to the ethanol solution is 1 g:20 mL), mix evenly, heat under reflux for 2 h, evaporate the extract by rotary evaporation and then dry it to obtain Litsea cubeba extract.

[0055] Preparation method: Weigh each raw material by mass percentage, mix them evenly, and then put them into a grinder and grind until the particle size reaches 5 μm to obtain a composition (suspension agent) for controlling Spodoptera frugiperda.

[0056] Example 4

[0057] Same as Example 1, the difference is only that the composition for controlling Spodoptera frugiperda is composed of the following components by mass percentage: Chlorantraniliprole 25%, marine cyanobacterium extract 10%, Litsea cubeba extract 5%, wetting agent (sodium dodecylbenzenesulfonate and sophora powder with a mass ratio of 1:1) 3%, thickening agent (xanthan gum and polyvinylpyrrolidone with a mass ratio of 1:1) 1%, dispersant (sodium lignosulfonate and benzyl methacrylate with a mass ratio of 1:1) 5%, defoaming agent (organosilicon defoaming agent) 0.5%, and add water to make up to 100%.

[0058] Comparative Example 1

[0059] Same as Example 1, the difference is only that the marine cyanobacterium extract is replaced with an equal mass percentage of Litsea cubeba extract.

[0060] Comparative Example 2

[0061] Same as Example 1, the difference is only that the Litsea cubeba extract is replaced with an equal mass percentage of marine cyanobacterium extract.

[0062] Comparative Example 3

[0063] Same as Example 1, the difference is only that the marine cyanobacterium extract and Litsea cubeba extract are replaced with an equal mass percentage of Chlorantraniliprole.

[0064] Comparative Example 4

[0065] Same as Example 1, the difference is only that the composition for controlling Spodoptera frugiperda is composed of the following components by mass percentage: Chlorantraniliprole 30%, marine cyanobacterium extract 3%, Litsea cubeba extract 3%, wetting agent (sodium dodecylbenzenesulfonate) 3%, thickening agent (xanthan gum) 1%, dispersant (sodium lignosulfonate) 5%, defoaming agent (organosilicon defoaming agent) 0.5%, and add water to make up to 100%.

[0066] Effect Example 1

[0067] At the peak of the low-instar stage of Spodoptera frugiperda larvae in the corn field, on the afternoon of June 8th, spray the compositions prepared in Examples 1-4 and Comparative Examples 1-4 of the present invention once (the liquid volume of the medicine is 30 kg / 667 m 2 , containing 3.0 g of active ingredient). After spraying for 7 days (after applying the medicine), count and calculate the control effect, and the results are shown in Table 1.

[0068] Spray clear water in the control area as a blank control group.

[0069]

[0070]

[0071] Table 1 Control effect

[0072]

[0073] By comparing Example 1 and Comparative Example 3, it is found that the present invention reduces the dosage of chlorantraniliprole and can still ensure the control effect against Spodoptera frugiperda, which is equivalent to that of Comparative Example 3 that only uses chlorantraniliprole as the active substance, achieving the technical effect of reducing the dosage of chemical pesticides and thus delaying the generation of drug resistance; moreover, the active substances used in the present invention, the extract of marine cyanobacteria and the extract of Litsea cubeba, are environmentally friendly and safe.

[0074] The embodiments described above are only used to describe the preferred mode of the present invention and do not limit the scope of the present invention. Without departing from the design spirit of the present invention, various deformations and improvements made by those of ordinary skill in the art to the technical solution of the present invention shall fall within the protection scope determined by the claims of the present invention.

Claims

1. A composition for controlling Spodoptera frugiperda, characterized in that, It comprises components in the following mass percentages: 15-40% of active ingredients and 60-85% of excipients; The active ingredients include chlorantraniliprole, marine cyanobacteria extract and Litsea cubeba extract; the mass ratio of chlorantraniliprole, marine cyanobacteria extract and Litsea cubeba extract is (5-8):(2-3):1; The preparation method of the marine cyanobacteria extract specifically comprises: (1) Activate the marine cyanobacteria and inoculate them in a liquid medium for culture to obtain a marine cyanobacteria fermentation broth; (2) Centrifuge the marine cyanobacteria fermentation broth to obtain marine cyanobacteria cells. Add water to the marine cyanobacteria cells, ultrasonically disrupt them, extract with ethyl acetate 2-3 times, and then extract with n-butanol 2-3 times. Combine the extracts to obtain the marine cyanobacteria extract; In step (1), the temperature of the culture is 28±2°C, the culture time is 10-15 d, and the rotation speed of the culture is 100-150 r / min; in step (2), the rotation speed of the centrifugation is 3000-3500 r / min, and the time is 10-12 min; the power of the ultrasonic disruption is 200-300 W, and the time is 5-8 min; The preparation method of the Litsea cubeba extract specifically comprises: dry and grind the stems and leaves of Litsea cubeba into powder, add an ethanol solution for heating under reflux extraction, rotary evaporate the extract and then dry it to obtain the Litsea cubeba extract; the volume fraction of the ethanol solution is 75-85%; the time of the heating under reflux extraction is 2-3 h; The application method of the composition is spraying.

2. The composition according to claim 1, characterized in that, The excipients include a wetting agent, a thickening agent, a dispersing agent, an antifoaming agent and water.

3. The composition according to claim 2, characterized in that, The wetting agent includes one or more of fatty alcohol polyoxyethylene ether, sodium dodecylbenzenesulfonate and sophora powder; the thickening agent includes one or more of hydroxyethyl cellulose, xanthan gum and polyvinylpyrrolidone; the dispersing agent includes one or more of sodium lignosulfonate, benzyl methacrylate and polycarboxylate; the antifoaming agent is an organosilicon antifoaming agent.

4. A preparation method of the composition according to any one of claims 1 to 3, characterized in that, It comprises the following steps: Weigh each raw material according to the mass percentage, grind, mix and homogenize to obtain the composition for controlling Spodoptera frugiperda.

5. An application of the composition according to any one of claims 1 to 3 in controlling Spodoptera frugiperda in crop fields.

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