Multi-channel piston type micro-fluidic immunoassay chip

Abstract

The application relates to the microfluidic technology field and provides a multi-channel piston type microfluidic immune detection chip, which comprises a chip body and a driving piece, the chip body comprises a sample feeding piece, a waste liquid pool and a detection unit, the waste liquid pool is in communication with the detection unit and the driving piece respectively, a clamping groove and an air inlet are further arranged on the chip body respectively, the air inlet is in communication with the detection unit through the clamping groove to form a first communication part, the sample feeding piece is in communication with the waste liquid pool through the clamping groove to form a second communication part, a piston assembly is further arranged, the piston assembly is slidably arranged in the clamping groove, the piston assembly is located on the communication paths of the first communication part and the second communication part, and a connecting piece for constructing the communication path is arranged on the piston assembly. The application has the effect of conveniently switching any specified channel for sample detection.

Description

TECHNICAL FIELD

[0001] The present application relates to the field of microfluidic technology, in particular to a multi-channel piston type microfluidic immunoassay chip. BACKGROUND

[0002] Microfluidic technology can integrate sample preparation, reaction, separation and other basic operation units in the biochemical analysis process on a chip to automatically complete the analysis process, which has the advantages of less sample consumption, fast detection speed and simple operation, and is often used in marker detection in the medical field to diagnose related diseases.

[0003] The existing microfluidic detection chip adopts a single piece or a multi-channel mode. When a single piece is used for detection, the detection efficiency is low, and the sample is wasted. The multi-channel mode is the mainstream detection mode. The main process is that the sample to be tested enters the chip from the sample inlet, flows along the length direction of the main flow channel under the guidance of the driving piece, and is guided by the driving piece to flow into each microchannel and flow towards the waste pool. When flowing through the detection unit, the sample to be tested in the sample to be tested reacts with the substances in the detection unit, and then is scanned and detected by an external scanner. After detection, it flows into the waste pool for treatment.

[0004] However, in the multi-channel mode, the sample flow is mostly directly and entirely flowed into each microchannel, or the sample flow is sequentially flowed into each microchannel for sample detection. When the sample flow is entirely and simultaneously flowed into the corresponding microchannels, unified sample detection is provided. When the sample flow is sequentially flowed into each microchannel, sample detection is provided. When it is necessary to provide self-selected and specified microchannels for detection, or to select different numbers and arbitrarily specified microchannels for sample detection, it is difficult to self-select, which reduces the accuracy of the detection results. SUMMARY

[0005] In order to improve the problem that it is difficult to self-select and specify the number of channels for sample detection, the present application provides a multi-channel piston type microfluidic immunoassay chip.

[0006] The multi-channel piston type microfluidic immunoassay chip provided by the present application adopts the following technical scheme:

[0007] A multi-channel piston type microfluidic immunoassay chip, comprising a chip body and a driving piece, the chip body comprising a sample inlet piece, a waste pool and a detection unit, the waste pool being in communication with the detection unit and the driving piece, characterized in that the chip body further comprises a clamping groove and an air inlet, the air inlet being in communication with the detection unit through the clamping groove to form a first communication part; the sample inlet piece is in communication with the waste pool through the clamping groove to form a second communication part.

[0008] Further comprising a piston assembly, the piston assembly is slidably arranged in the clamping groove, the piston assembly is located on the communication path of the first communication part and the communication path of the second communication part, the piston assembly is provided with a connecting piece for building the communication path, the connecting piece and the first communication part and the connecting piece and the second communication part are controlled by the sliding of the piston assembly.

[0009] The detection unit and the piston assembly are provided with at least two.

[0010] Through the above technical scheme, multiple piston assemblies cooperate with multiple clamping grooves to control the detection of multiple detection units, and a single piston assembly can be individually slid and transported, or multiple piston assemblies can be simultaneously slid and transported, which can be selected by the user, and is not limited to individual operation or all operation, so that the comparison and reference of the detection quantity and the detection result are more flexible.

[0011] Optionally, when the connecting piece is located in the second communication part, the piston assembly slides along the clamping groove by a preset distance, the communication path of the second communication part is disconnected to form a disconnected path, and the connecting piece is connected to the communication path of the first communication part by continuously sliding along the clamping groove by a preset distance.

[0012] Through the above technical scheme, the sliding position is determined by sliding a preset distance, and the connecting piece is switched more accurately and stably by sliding any preset distance.

[0013] Optionally, further comprising a cleaning assembly arranged on the chip main body, the cleaning assembly is communicated with the waste liquid pool through the clamping groove to form a third communication part, the piston assembly is located on the communication path of the third communication part, and the connecting piece and the third communication part are controlled by the sliding of the piston assembly.

[0014] Through the above technical scheme, when the detection is completed, the channels of each detection unit in the chip main body are very inconvenient to clean, the cleaning liquid in the cleaning part is extracted by forming negative pressure in the detection unit through the first driving piece, the connecting piece is butted against the third communication part by moving the piston assembly along the clamping groove, the path of the third communication part is communicated, the cleaning liquid flows into each part of the detection unit through the connecting piece of the piston assembly, and finally flows into the waste liquid pool for unified treatment, so as to achieve the effect of convenient cleaning.

[0015] Optionally, the cleaning assembly comprises a cleaning piece, an overflow pool and a liquid passing piece; the cleaning piece, the overflow pool and the liquid passing piece are all installed on the chip main body; one end of the cleaning piece is communicated with the overflow pool through the liquid passing piece, and the other end is communicated with the clamping groove through the liquid passing piece; the relative positions of the communication positions of the liquid passing piece and the clamping groove are provided with liquid inlets of the detection units.

[0016] By adopting the technical scheme, when the detection unit needs to be cleaned, the cleaning liquid in the cleaning member is squeezed out or discharged, the cleaning liquid enters the liquid passing member for temporary storage, the piston assembly slides along the clamping groove, the connecting member is moved to the liquid passing member to make the third communication part communicate, the piston assembly is stopped from moving, the driving member forms negative pressure, the cleaning liquid in the liquid passing member is sucked into the detection unit through the connecting member for cleaning, and finally the cleaned cleaning liquid is collected in the waste liquid pool for unified recycling.

[0017] Optionally, the overflow pool is provided with an air hole.

[0018] By adopting the technical scheme, the overflow pool is provided with an air hole for forming a passage of the driving member, the driving member is combined with the air hole of the overflow pool to form negative pressure in the detection unit, and the flow of the cleaning liquid is facilitated.

[0019] Optionally, the liquid passing member between the overflow pool and the cleaning member comprises at least one bend.

[0020] By adopting the technical scheme, the bend is arranged to expand the storage space between the overflow pool and the cleaning member, so that sufficient cleaning liquid can be ensured to clean the detection unit, and the more the bends are arranged, the larger the storage capacity is.

[0021] Optionally, the positioning structure is further arranged, the positioning structure is arranged on the chip body near the outlet of the clamping groove, and the positioning structure is connected with the piston assembly.

[0022] By adopting the technical scheme, the positioning assembly is arranged, the piston assembly can be directly and accurately determined to slide by the positioning assembly, and the detection efficiency and accuracy are improved.

[0023] Optionally, the positioning structure comprises a fixing member, a pushing member, a movable member and a clamping member, the fixing member is arranged on the chip body and located near the outlet of the clamping groove, at least two clamping interfaces are arranged on the fixing member, the movable member is arranged on the piston assembly, the pushing member is movably connected between the fixing member and the movable member, the clamping member is arranged on the pushing member, and at least two groups of clamping members are arranged.

[0024] By adopting the technical scheme, when the sample injection member needs to be inserted into the liquid sample, the user pushes the piston assembly through the positioning structure, the clamping piece is clamped by the fixing piece after reaching the preset distance, the position of the piston assembly is fixed, the rotating pusher is pushed along the movable piece, the clamping piece is switched to be aligned with the clamping groove, the piston assembly can be continuously pushed, and the connecting piece of the piston assembly is switched to the first communication part, the disconnection path and the second communication part in turn and repeatedly through the positioning structure, so that the sliding distance of the piston assembly is not manually and slowly adjusted, the detection efficiency is improved, and the accuracy of the butt joint is also ensured.

[0025] Optionally, the clamping pieces in each group are arranged at intervals of a preset distance.

[0026] By adopting the technical scheme, when each group of clamping pieces is depressed by a preset distance, the communication state of the connecting piece is switched, and the sliding distance of the piston assembly can be accurately ensured to be the preset distance through the clamping piece.

[0027] Optionally, the clamping pieces in each group are arranged in a staggered manner along the vertical direction of the pusher.

[0028] By adopting the technical scheme, each group of clamping pieces arranged in a staggered manner can be clamped by the fixing piece when being depressed, so that the preset distance can be accurately positioned.

[0029] In summary, the present application has at least one of the following beneficial technical effects:

[0030] 1. The path switching is realized by pushing and pulling the piston assembly, a plurality of piston assemblies cooperate with a plurality of clamping grooves to control the detection of a plurality of detection units, a single piston assembly can be individually slid and conveyed, or a plurality of piston assemblies can be simultaneously slid and conveyed, which can be selected by the user, and the detection quantity and the comparison and reference of the detection results are more flexible.

[0031] 2. The cleaning liquid in the cleaning assembly is extracted by forming a negative pressure in the detection unit through the driving piece, and the piston assembly is moved along the clamping groove, the cleaning assembly and the detection unit are communicated, and the driving piece drives the cleaning liquid to flow into each component of the detection unit through the connecting piece of the piston assembly, so that the cleaning effect is achieved.

[0032] 3. The positioning structure is arranged on the piston assembly, the sliding position of the piston assembly can be more directly and accurately determined by using the positioning structure, and the detection efficiency and accuracy are improved. BRIEF DESCRIPTION OF DRAWINGS

[0033] Figure 1 is a cross-sectional structure schematic view of the first communication state of the first communication part in Embodiment 1 of the present application.

[0034] Figure 2is a cross-sectional structure schematic diagram of a disconnected path state in embodiment 1 of the present application;

[0035] Figure 3 is a cross-sectional structure schematic diagram of a first communication part communication state in embodiment 1 of the present application;

[0036] Figure 4 is a three-dimensional structure schematic diagram of a chip main body in embodiment 2 of the present application;

[0037] Figure 5 is a cross-sectional structure schematic diagram of a first communication part communication state in embodiment 2 of the present application;

[0038] Figure 6 is a cross-sectional structure schematic diagram of a positioning structure in a first communication part communication state in embodiment 3 of the present application;

[0039] Figure 7 is a three-dimensional structure schematic diagram of a positioning structure in a third communication part communication state in embodiment 3 of the present application;

[0040] The marks in the drawings are: 1, chip main body, 11, sample inlet, 12, waste liquid pool, 13, detection unit, 131, reaction pool, 132, scanning pool, 133, reaction part, 14, clamping groove, 15, gas inlet, 2, driving part, 3, piston assembly, 31, connecting part, 4, cleaning assembly, 41, cleaning part, 42, overflow pool, 43, liquid passing part, 5, positioning structure, 51, fixing part, 52, pushing part, 53, movable part, 54, clamping part, 55, clamping port. DETAILED DESCRIPTION

[0041] The following will be described in detail in combination with the drawings Figure 1 - the drawings Figure 7 The present application will be further described in detail.

[0042] The embodiment of the present application discloses a multi-channel piston type microfluidic immunodetection chip.

[0043] Embodiment 1:

[0044] A multi-channel piston type microfluidic immunodetection chip, referring to Figure 1, including the chip body and the driving member 2, the chip body includes the sample inlet member 11, the waste liquid pool 12 and the detection unit 13, the waste liquid pool 12 is communicated with the detection unit 13 and the driving member 2 respectively. Wherein, the chip body 1 is the main bearing tool for installing and detecting the detection structure, the sample inlet member 11 is provided with a sample port for the sample to enter, the sample port can be sealed by a sealing cover, the driving member 2 is the main driving element for the liquid sample flow, which can adopt a power source such as a gas pump or a piston syringe that can form negative pressure, and the embodiment adopts a piston syringe, the detection unit 13 provides a reaction and detection place for the liquid sample, the number of the detection unit 13 is determined according to actual needs, and the embodiment sets three detection units 13. The waste liquid pool 12 can communicate the detection unit 13 with the sample inlet member 11, the driving member 2 can pump the waste liquid pool 12 to form negative pressure in the waste liquid pool 12, so as to facilitate the circulation of the liquid sample, and the waste liquid pool 12 finally receives the waste liquid flowing out of the detection unit 13.

[0045] The sample inlet member 11 and the detection unit 13 are both externally connected with sensors, and the external sensors are electrically connected with the driving member 2, the sensors can detect the amount of the circulating liquid sample in the sample inlet member 11 and the detection unit 13, when the amount reaches a sufficient detection amount, the external sensor sends an electric signal to the driving member 2, and the driving member 2 can be stopped.

[0046] Wherein, the chip body is further provided with a clamping groove 14 and an air inlet 15 respectively, the air inlet 15 is communicated with the detection unit 13 through the clamping groove 14 to form a first communication part, and the sample inlet member 11 is communicated with the waste liquid pool 12 through the clamping groove 14 to form a second communication part.

[0047] Further comprising a piston assembly 3, the piston assembly 3 is slidably arranged in the clamping groove 14, the piston assembly 3 is located on the communication path of the first communication part and the communication path of the second communication part, the piston assembly 3 is provided with a connecting piece 31 for building the communication path, and the connecting piece 31 is connected and disconnected between the first communication part and the second communication part through the sliding of the piston assembly 3. The number of the piston assembly 3 in the embodiment is three, and each piston assembly 3 corresponds to a corresponding detection unit 13 and air inlet 15, the communication state of the first communication part is the initial state of the piston assembly 3, in the communication state of the first communication part, the air inlet 15 is not communicated with the detection unit 13, and the sample inlet member 11 is in the communication state with the waste liquid pool 12, the waste liquid pool 12 is pumped by the driving member 2 to form negative pressure, so that the liquid sample stored at the sample port of the sample inlet member 11 is pumped into the sample inlet member 11 through the connecting piece 31, the external sensor is arranged at the position close to the waste liquid pool 12 and communicated with the last piston assembly 3 of the sample inlet member 11, when the external sensor detects that the entering amount of the liquid sample reaches the expectation, an electric signal is sent to stop the driving member 2 from working, at this time, the liquid sample is stored in all the piston assemblies 3.

[0048] Referring to Figure 2As shown, when the connecting piece is located in the second communication part, the piston assembly slides along the clamping groove by a preset distance, the communication path of the second communication part is disconnected to form a disconnected path, and the connecting piece accesses the communication path of the first communication part when it continues to slide along the clamping groove by the preset distance; after the driving member 2 stops pumping, all piston assemblies 3 are pressed down, and the sample inlet 11 forms a disconnected path, so that the liquid sample in all piston assemblies 3 cannot flow.

[0049] Referring to Figure 3 As shown, in the disconnected path, any piston assembly 3 slides along the clamping groove 14 by a preset distance, and any piston assembly 3 is in one-to-one communication with the corresponding air inlet 15 and the corresponding detection unit 13 to form a first communication part; when it is necessary to detect any detection unit 13, the piston assembly 3 corresponding to the detection unit 13 to be detected is selected, the selected piston assembly 3 is pressed down, and the selected piston assembly 3 slides along the clamping groove 14 by a preset distance, so that the selected piston assembly 3 communicates the corresponding air inlet 15 and the corresponding detection unit 13 to form a first communication part; at this time, other piston assemblies 3 that are not pressed down are located in the disconnected path, so when the driving member 2 extracts the waste liquid pool 12 to form a negative pressure, the negative pressure state cooperates the liquid sample in the selected piston assembly 3 with the air inlet 15 to be extracted into the detection unit 13, so as to detect the sample of the selected detection unit 13.

[0050] A single piston assembly 3 can be individually slid along the corresponding clamping groove 14, or multiple piston assemblies 3 can be simultaneously slid along the corresponding clamping groove 14; the specific number of piston assemblies 3 to be operated can be selected by the user, such as one piston assembly 3, any two piston assemblies 3, or three piston assemblies 3, which are not limited to individual operation or all operation, so that the comparison and reference of the detection number and the detection result are more flexible.

[0051] Among them, the piston assembly 3 is a sliding moving tool, and the connecting piece 31 is a liquid sample receiving tool, which provides a receiving space for the liquid sample when it accesses the first communication part; when the piston assembly 3 slides along the clamping groove 14 by a preset distance, the piston assembly 3 drives the connecting piece 31 into the disconnected path and the first communication part in sequence, and the liquid sample in the connecting piece 31 can be extracted into the detection unit 13 through the cooperation of the driving member 2 and the air inlet 15 to detect the selected detection unit 13.

[0052] The user can selectively perform sample detection on multiple detection units 13 at the same time, and it is not limited to single or all detection units 13 at the same time, which improves the flexibility of sample detection.

[0053] The detection unit 13 comprises a reaction pool 131, a scanning pool 132 and a reaction member 133; the reaction member 133 is connected with the reaction pool 131, the scanning pool 132 and the waste liquid pool 12 in sequence, and the scanning pool 132 is connected with an external sensor sensing end; the driving member 2 forms negative pressure in the waste liquid pool 12 by suction, and there is a chemical substance, such as a freeze-dried reagent, in the reaction pool 131; the reaction member 133 can adopt a serpentine channel (S type) or a U-shaped channel, and the embodiment adopts a serpentine channel; under the negative pressure state, the liquid sample enters the reaction member 133 through the connecting member 31, melts the freeze-dried reagent in the reaction pool 131, and mixes and reacts in the serpentine channel; after the mixing and reaction are completed, the sample enters the scanning pool 132; the sensor stops the driving member 2 after detecting that the liquid sample enters the scanning pool 132, starts the incubation time counting, and scans the scanning pool 132 by an external scanning instrument to obtain a detection result when the liquid sample is fully reacted.

[0054] The connecting member 31, the sample inlet member 11 and the reaction member 133 can adopt a cavity structure or a tubular structure.

[0055] The implementation principle of the embodiment 1 of the application comprises that all the piston assemblies 3 are in an initial state located in the second communication part; the driving member 2 forms negative pressure in the waste liquid pool 12; the liquid sample is sucked into the sample inlet member 11 and the piston assemblies 3 through the sample inlet member 11; after the piston assemblies 3 move along the clamping groove 14 by a preset distance, all the piston assemblies 3 enter the disconnected path; in the disconnected path, any piston assembly 3 is selected to continue to slide along the clamping groove 14 by a preset distance; the selected piston assembly 3, the air inlet 15 and the detection unit 13 form the first communication part; the driving member 2 forms negative pressure in the waste liquid pool 12; at this time, other piston assemblies 3 not under pressure are located in the disconnected path; the liquid sample in the selected piston assembly 3 is sucked into the detection unit 13 in cooperation with the air inlet 15 under the negative pressure state, so as to detect the sample in the selected detection unit 13; the specific operation of the piston assemblies 3 is specified and any number, which can be selected by the user, such as one piston assembly 3, any two piston assemblies 3 or three piston assemblies 3, is not limited to only single operation or all operation, so that the detection quantity and the detection reference are more flexible.

[0056] Embodiment 2

[0057] Reference Figure 5 and Figure 6As shown, the embodiment 2 of the present application is optimized on the basis of the embodiment 1, including the following optimizations, and further including a cleaning assembly 4 arranged on the chip main body 1, the cleaning assembly 4 is in communication with the waste liquid pool 12 through the clamping groove 14 to form a third communication part, the piston assembly 3 is located on the communication path of the third communication part, and the connection between the connecting piece 31 and the third communication part is controlled by the sliding of the piston assembly 3. When the detection is completed, the detection unit 13 in the chip main body 1 is very inconvenient to clean, and therefore the cleaning assembly 4 is arranged to assist in cleaning the detection unit 13.

[0058] Specifically, the cleaning liquid in the cleaning assembly 4 is extracted by forming a negative pressure in the detection unit 13 through the driving piece 2, and is moved along the clamping groove 14 through the piston assembly 3, the connecting piece 31 is connected to the third communication part, so that the third communication part is in a communication state, and the cleaning assembly 4 and the detection unit 13 are communicated, the cleaning liquid is driven by the driving piece 2 to flow to each component of the detection unit 13 through the connecting piece 31 of the piston assembly 3, and finally flows into the waste liquid pool 12 for unified treatment.

[0059] Specifically, the cleaning assembly 4 includes a cleaning piece 41, an overflow pool 42 and a liquid passage piece 43; the cleaning piece 41, the overflow pool 42 and the liquid passage piece 43 are all installed on the chip main body 1; one end of the cleaning piece 41 is in communication with the overflow pool 42 through the liquid passage piece 43, and the other end is in communication with the clamping groove 14 through the liquid passage piece 43; the relative position of the communication part of the liquid passage piece 43 and the clamping groove 14 is provided with a liquid inlet of the detection unit 13. Specifically, the cleaning piece 41 can be a rubber bag or a rubber cover containing cleaning liquid, the overflow pool 42 is used for storing and processing excess cleaning liquid, and the overflow pool 42 is provided with air holes, the cleaning piece 41 is in communication with the overflow pool 42 through the liquid passage piece 43, and the other end of the cleaning piece 41 is connected to the clamping groove 14, the communication part of the liquid passage piece 43 and the clamping groove 14 is located below the corresponding air inlet 15 along the bottom of the clamping groove 14, and the distance from the air inlet 15 is a predetermined distance.

[0060] The liquid passage piece 43 between the overflow pool 42 and the cleaning piece 41 contains at least one bend, and the bend is arranged to expand the storage space between the overflow pool 42 and the cleaning piece 41, so as to ensure that there is enough cleaning liquid to clean the detection unit 13, and the more bends of the liquid passage piece 43 are arranged, the larger the storage capacity is.

[0061] The implementation principle of the embodiment 2 of the present application comprises: when the detection unit 13 needs to be cleaned, all the piston assemblies 3 are pushed to the disconnection path, the cleaning liquid in the cleaning member 41 is squeezed out or discharged, the cleaning liquid enters the liquid passing member 43 through the air holes of the overflow pool 42 and is temporarily stored, the piston assemblies 3 slide along the clamping groove 14, the connecting member 31 is moved to the liquid passing member 43 and stops moving the piston assemblies 3, the connecting member 31 connects the liquid passing member 43 with the liquid inlet of the detection unit 13, because the air holes for forming the passage of the driving member 2 are arranged on the overflow pool 42, the driving member 2 cooperates with the air holes of the overflow pool 42 to form negative pressure in the detection unit 13, so that the cleaning liquid in the liquid passing member 43 is sucked into the detection unit 13 through the connecting member 31 to clean, and finally the cleaned cleaning liquid is collected in the waste liquid pool 12 for unified recycling.

[0062] Embodiment 3

[0063] Referring to Figure 6 The embodiment 3 is optimized on the basis of the embodiment 1, and the specific optimization content comprises: the positioning structure 5 is further arranged, the positioning structure 5 is arranged on the chip main body 1 at the side close to the outlet of the clamping groove 14, and the positioning structure 5 is connected with the piston assembly 3; when the piston assembly 3 is directly pushed, the specific position of the piston assembly 3 cannot be clearly understood, and the connecting member 31 on the piston assembly 3 cannot be accurately connected with each member, for example, only half of the connecting member 31 is connected with each member, which is easy to cause the liquid sample to leak, therefore, the positioning structure 5 is arranged on the piston assembly 3, the piston assembly 3 is directly and accurately determined to slide by the positioning structure 5, and the detection efficiency and accuracy are improved.

[0064] The positioning structure 5 comprises the fixing member 51, the pushing member 52, the movable member 53 and the clamping member 54; the fixing member 51 is arranged on the chip main body 1 and located at the side close to the outlet of the clamping groove 14, at least two clamping interfaces 55 are arranged on the fixing member 51; the movable member 53 is arranged at least twice and arranged on the corresponding piston assembly 3; the pushing member 52 is movably connected with the fixing member 51 and the movable member 53; the clamping member 54 is arranged on the pushing member 52, and the clamping member 54 is arranged at least twice; the pushing member 52 drives the piston assembly 3 to slide along the clamping groove 14 by a preset distance, the clamping member 54 is clamped with the fixing member 51, the pushing member 52 rotates along the movable member 53 by a preset angle, and the clamping member 54 penetrates through the clamping interface 55 and continues to slide.

[0065] Specifically, the fixing member 51 can adopt a fixing frame, a through hole for the sliding of the pushing member 52 is formed on the fixing frame, the through hole is communicated with the clamping port 55, the clamping port 55 can be a strip-shaped port, the clamping member 54 can be penetrated by the pushing member 52, and the pushing member 52 slides along the through hole and penetrates the clamping port 55, the movable member 53 can be a bearing seat or a pivoting port, and the pushing member 52 is a push rod or a tubular structure, and the like can be applicable, as long as the movable member 53 is movably connected, the movable member 53 of the embodiment adopts a bearing seat, the pushing member 52 adopts a push rod, the push rod is pivoted with the bearing seat, so that the rotation of the push rod along the bearing seat will not affect the sliding of the piston assembly 3, and the rotation of the push rod can change the position of the clamping member 54, when the clamping member 54 and the clamping port 55 are not aligned, the clamping member 54 clamps the pushing member 52 on the fixing member 51, so that the pushing member 52 cannot continue to push, when the clamping member 54 is produced and installed, the clamping position of the clamping member 54 is consistent with the position of the connecting member 31 connected to the sampling member 11, the clamping member 54 can be provided in multiple groups, when referring to the first embodiment, three groups of clamping members 54 are provided, and when referring to the second embodiment, four groups of clamping members 54 are provided.

[0066] The interval of each group of clamping members 54 is a preset distance, so that when each group of clamping members 54 is pressed by a preset distance, the communication state of the connecting member 31 will be switched, and the sliding distance of the piston assembly 3 can be accurately ensured by the clamping member 54.

[0067] The position of the clamping member 54 of the first group clamped by the fixing member 51 corresponds to the communication state of the second communication part, which is the initial position of the connecting member 31, the position of the clamping member 54 of the second group clamped by the fixing member 51 corresponds to the disconnection path, which can switch the connecting member 31 to the disconnection path, the position of the clamping member 54 of the third group clamped by the fixing member 51 corresponds to the communication state position of the first communication part, which can switch the connecting member 31 to the first communication part.

[0068] When the user pushes the piston assembly 3, all the pushing members 52 are pressed down directly, and all the first set of locking members 54 are locked by the fixing member 51. The connecting member 31 is connected to the second connecting part. The liquid sample is drawn into the connecting member 31 by the driving member 2. The piston assembly 3 needs to be pressed down further, so the pushing members 52 are rotated along the movable member 53 so that the first set of locking members 54 are aligned with the card interface 55. After all the locking members 54 on the pushing members 52 are aligned with the card interface 55, all the pushing members 52 are pressed down further, and all the second The snap-fit ​​connector 54 is held in place by the fixing member 51, and all connectors 31 are in the disconnection path. Select the piston assembly 3 to be tested, rotate the corresponding push member 52 to align the corresponding snap-fit ​​connector 54 with the corresponding snap-fit ​​interface 55, and continue to press down the selected push member 52 until the selected push member 52 is held in place by the third snap-fit ​​connector 54 and the fixing member 51. The selected connector 31 enters the corresponding first communication part communication state, and the liquid sample in the connector 31 is extracted into the detection unit 13 for testing by the drive member 2.

[0069] When referring to Example 2, the corresponding reference is... Figure 7 As shown, four sets of snap-fit ​​parts 54 are provided. The position of the snap-fit ​​parts 54 in the fourth set is locked by the fixing part 51 and corresponds to the communication state of the third connecting part. When it is necessary to continue to press down the piston assembly 3 to switch to the cleaning part 41, the snap-fit ​​parts 54 in the third set are rotated along the movable part 53 by the pushing part 52 to align with the snap-fit ​​interface 55. The pushing part 52 is pressed down until the fourth set of snap-fit ​​parts 54 is locked with the fixing part 51. The connecting part 31 is connected to the third connecting part. The cleaning fluid is drawn into the connecting part 31 and the detection unit 13 for cleaning by the driving part 2.

[0070] With the positioning structure 5 in place, the piston assembly 3 can be moved directly by a preset distance without the need for manual adjustment of its sliding distance, thus improving detection efficiency and ensuring docking accuracy.

[0071] Each set of latching parts 54 can be staggered along the vertical direction of the pusher 52, or they can be aligned in the same position along the vertical direction of the pusher 52. When staggered, it is convenient to press down the pusher 52 without rotating the pusher 52, so that the next set of latching parts 54 can be locked with the fixing part 51. However, when lifting, it is necessary to rotate the latching parts 54 along the movable part 53 and align them with the locking interface 55 before lifting. When aligned, after pressing down the pusher 52, it is necessary to rotate the pusher 52 along the movable part 53 to make each set of latching parts 54 misaligned with the locking interface 55, so that the latching parts 54 can be locked by the fixing part 51. However, when lifting the piston assembly 3 and the pusher 52, it is not necessary to rotate the latching parts 54. It is sufficient to align one of the latching parts 54 with the locking interface 55 to lift them all directly. The specific setting depends on the requirements. In this embodiment 3, staggered setting is adopted. When staggered, the included angle between the two sets of latching parts 54 can be 30-90 degrees. In this embodiment, 90 degrees is adopted.

[0072] The implementation principle of the embodiment 3 of the application comprises: when it is needed to connect the sample injection member 11 to the liquid sample, the user pushes the piston assembly 3 through the positioning structure 5, the piston assembly 3 is clamped by the positioning structure 5 after reaching a preset distance, and the piston assembly 3 can be continuously pushed through rotation switching, and the connecting piece 31 of the piston assembly 3 is switched to the first communication part, the disconnection path and the second communication part through the positioning structure 5 in turn and repeatedly, so that the sliding distance of the piston assembly 3 is not manually and slowly adjusted, the detection efficiency is improved, and the accuracy of the connection is also guaranteed.

[0073] The embodiments of the specific embodiment are the preferred embodiments of the application, and are not intended to limit the protection scope of the application, wherein the same parts are indicated by the same reference numerals. Therefore: any equivalent changes made according to the structure, shape and principle of the application should be covered in the protection scope of the application.

Claims

1. A multi-channel, piston-type microfluidic immunoassay chip, characterized in that, The chip body (1) includes a sample injection member (11), a waste liquid pool (12) and a detection unit (13), the waste liquid pool (12) is respectively communicated with the detection unit (13) and the driving member (2), the chip body (1) is further respectively provided with a clamping groove (14) and an air inlet (15), the air inlet (15) is communicated with the detection unit (13) through the clamping groove (14) to form a first communication part; the sample injection member (11) is communicated with the waste liquid pool (12) through the clamping groove (14) to form a second communication part; Further comprising a piston assembly (3), the piston assembly (3) is slidably arranged in the clamping groove (14), the piston assembly (3) is located on the communication path of the first communication part and the communication path of the second communication part, the piston assembly (3) is provided with a connecting piece (31) for building a communication path, the connecting between the connecting piece (31) and the first communication part, the connecting between the connecting piece (31) and the second communication part is controlled by the sliding of the piston assembly (3); The detection unit (13) and the piston assembly (3) are each provided with at least two; Further comprising a cleaning assembly (4) arranged on the chip body (1), the cleaning assembly (4) is communicated with the waste liquid pool (12) through the clamping groove (14) to form a third communication part, the piston assembly (3) is located on the communication path of the third communication part, the communication between the connecting piece (31) and the third communication part is controlled by the sliding of the piston assembly (3); Further comprising a positioning structure (5), the positioning structure (5) is mounted on the chip body (1) and located on the side close to the outlet of the clamping groove (14), and the positioning structure (5) is connected with the piston assembly (3) The positioning structure (5) comprises a fixing member (51), a pushing member (52), a movable member (53) and a clamping member (54); the fixing member (51) is mounted on the chip body (1) and located on the side close to the outlet of the clamping groove (14), at least two clamping interfaces (55) are formed in the fixing member (51); the movable member (53) is provided with at least two and is respectively mounted on the piston assembly (3); the pushing member (52) is movably connected with the movable member (53) through the fixing member (51); the clamping member (54) is mounted on the pushing member (52), and the clamping member (54) is provided with at least two groups; the pushing member (52) drives the piston assembly (3) to slide along the clamping groove (14) by a preset distance, so that the clamping member (54) is clamped with the fixing member (51), the pushing member (52) rotates by a preset angle along the movable member (53), so that the clamping member (54) penetrates through the clamping interface (55) and continues to slide.

2. The multi-channel, piston-type microfluidic immunoassay chip of claim 1, wherein, When the connecting piece (31) is located in the second communication part, the piston assembly (3) slides along the clamping groove (14) by a preset distance, the communication path of the second communication part is disconnected to form a disconnected path, and the connecting piece (31) is connected to the communication path of the first communication part by sliding along the clamping groove (14) by a preset distance.

3. The multi-channel, piston-type microfluidic immunoassay chip of claim 1, wherein, The cleaning assembly (4) comprises a cleaning piece (41), an overflow pool (42) and a liquid passing piece (43); the cleaning piece (41), the overflow pool (42) and the liquid passing piece (43) are all installed on the chip main body (1); one end of the cleaning piece (41) is communicated with the overflow pool (42) through the liquid passing piece (43), and the other end is communicated with the clamping groove (14) through the liquid passing piece (43); the relative position of the communication position of the liquid passing piece (43) and the clamping groove (14) is provided with the liquid inlet of the detection unit (13).

4. The multi-channel, piston-type microfluidic immunoassay chip of claim 3, wherein, A gas hole is formed on the overflow pool (42).

5. The multi-channel, piston-type microfluidic immunoassay chip of claim 3, wherein, The liquid passing piece (43) between the overflow pool (42) and the cleaning piece (41) comprises at least one bending.

6. The multi-channel, piston-type microfluidic immunoassay chip of claim 1, wherein, The interval of each group of the clamping pieces (54) is a preset distance.

7. The multi-channel, piston-type microfluidic immunoassay chip of claim 1, wherein, Each group of the clamping pieces (54) are staggered along the vertical direction of the pushing piece (52).

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