Preparation method of Pickering breast joint bionic synovial fluid of traditional Chinese medicine volatile oil

Abstract

The invention discloses a method for preparing a Pickering emulsion bionic synovial fluid of Chinese herbal volatile oil, and relates to the technical field of biolubricants. The method comprises: adding nanoparticles to ultrapure water, and ultrasonically dispersing to obtain a nanoparticle dispersion; adding Chinese herbal volatile oil to the nanoparticle dispersion according to a certain volume fraction, and ultrasonically stirring to obtain a Pickering emulsion bionic synovial fluid of Chinese herbal volatile oil. The Pickering emulsion bionic synovial fluid of Chinese herbal volatile oil prepared by the invention has excellent friction reduction and anti-wear properties at the interface of artificial joints and articular cartilage, and can effectively remove active oxygen free radicals in the environmental system, and can inhibit and alleviate the onset of osteoarthritis from the two aspects of controlling mechanical stress and reducing oxidative stress.

Description

Technical Field

[0001] The invention relates to the technical field of biolubricants, in particular to a method for preparing Pickering breast joint bionic synovial fluid of traditional Chinese medicine volatile oil. Background Art

[0002] Osteoarthritis (OA) is a common degenerative joint disease. Patients often experience joint pain, deformity, and even motor dysfunction, which seriously affects their quality of life. Studies have shown that articular cartilage damage caused by mechanical stress and oxidative stress is the main cause of OA. Excessive mechanical stress can lead to excessive production of reactive oxygen species (ROS, including chemically active molecules containing oxygen such as hydroxyl, superoxide anion, hydrogen peroxide, and hypochlorite ion) in articular cartilage. Under normal physiological conditions, the generation and clearance of ROS in the body will be maintained in a dynamic equilibrium state, but when the amount of ROS produced exceeds the body's antioxidant system's ability to clear it, oxidative stress will occur and promote the expression of inflammatory cytokines, further causing oxidative damage to intracellular biomacromolecules, thereby inducing chondrocyte apoptosis. Therefore, regulating the balance of ROS in the joint cavity is of great significance for the treatment of osteoarthritis.

[0003] Synovial tissue can secrete an appropriate amount of synovial fluid to provide sufficient lubrication for the joint interface and reduce mechanical friction stress. This native synovial fluid is mainly composed of water, hyaluronic acid, phospholipids and lubricants. From the perspective of lubrication mechanism, hyaluronic acid can change the viscoelasticity of synovial fluid and play a role in fluid dynamic lubrication, and phospholipids and lubricants can be adsorbed on the cartilage interface and play a certain role in boundary lubrication. However, in the pathogenesis of OA, the damage or lack of cartilage tissue makes it unable to secrete lubricating substances such as hyaluronic acid, phospholipids and lubricants. In vitro injection of joint biolubricants is a traditional way to alleviate articular cartilage damage. At present, the main joint biolubricant used in clinical practice is hyaluronic acid. Although hyaluronic acid has good fluid dynamic lubrication properties at the joint interface, hyaluronic acid will break under boundary lubrication and lose its good lubrication effect. Therefore, hyaluronic acid cannot fully ensure that the joint interface is in a good lubrication state under various complex motion conditions. In addition, as an in vitro injection lubricant, exogenous hyaluronic acid will degrade in the joint cavity and be excreted from the body, which leads to the inability of hyaluronic acid as a joint lubricant to play a long-term lubricating role at the articular cartilage interface. Therefore, researching a long-acting biolubricant for joints with good lubrication effect is of great significance for the treatment of osteoarthritis. Summary of the invention

[0004] The present invention aims to provide a method for preparing a Pickering breast joint bionic synovial fluid of Chinese herbal volatile oil, and prepares a Pickering breast joint bionic synovial fluid of Chinese herbal volatile oil by using nanoparticles as a stabilizer. On the one hand, the excellent boundary lubrication performance of the nanoparticles is used to significantly improve the lubrication condition of the joint interface; on the other hand, the antioxidant effect of the Chinese herbal volatile oil is used to remove ROS generated at the joint interface. The Chinese herbal volatile oil Pickering emulsion bionic synovial fluid prepared by the present invention has excellent friction reduction and anti-wear performance at the interface of artificial joints and articular cartilage, and can effectively remove active oxygen free radicals in the environmental system, and can inhibit and alleviate the onset of osteoarthritis from the two aspects of controlling mechanical stress and reducing oxidative stress.

[0005] To achieve the above object, the present invention provides the following technical solutions:

[0006] The invention discloses a method for preparing Pickering breast joint bionic synovial fluid of traditional Chinese medicine volatile oil, and the method comprises the following steps:

[0007] Adding nanoparticles into ultrapure water and ultrasonically dispersing them to obtain a nanoparticle dispersion;

[0008] The Chinese herbal medicine volatile oil is added into the nanoparticle dispersion according to a certain volume fraction, and ultrasonic stirring is performed to obtain the Chinese herbal medicine volatile oil Pickering emulsion joint biomimetic synovial fluid.

[0009] In a possible embodiment, the nanoparticles are one or a combination of graphene oxide, fullerene, nanodiamond, carbon nanotube, nanosilicon dioxide and nanocellulose; wherein,

[0010] The mass ratio of oxygen-containing functional groups on the surface of the nanoparticles is 28% to 37%.

[0011] In a possible implementation, the conditions for the ultrasonic dispersion are:

[0012] The ultrasonic power is 150-1000W, and the ultrasonic dispersion time is 1-2h.

[0013] In a possible implementation manner, the concentration of the nanoparticle dispersion is 2.0 to 6.0 g / L.

[0014] In a possible implementation manner, the certain volume fraction is 10% to 20%.

[0015] In a possible embodiment, the Chinese medicine volatile oil is one or a combination of angelica volatile oil, chuanxiong rhizome volatile oil, pubescent volatile oil, cassia twig volatile oil, salvia miltiorrhiza volatile oil, eucommia ulmoides volatile oil, white peony root volatile oil, wormwood volatile oil, notopterygium root volatile oil, Achyranthes bidentata volatile oil, asarum volatile oil, qiannianjian volatile oil, turmeric volatile oil and houttuynia cordata volatile oil.

[0016] In a possible implementation, the conditions for the ultrasonic stirring are:

[0017] The ultrasonic power is 50-500W, the ultrasonic temperature is 30-70°C, and the ultrasonic stirring time is 0.05-1h.

[0018] Technical effects and advantages of the present invention:

[0019] First, the present invention uses nanoparticles as stabilizers to prepare a Chinese medicine volatile oil Pickering breast joint bionic synovial fluid. While serving as stabilizers, the nanoparticles also have good friction and anti-wear properties, effectively reducing friction damage at the joints.

[0020] Second, the Pickering breast joint bionic synovial fluid prepared by the present invention combines the antioxidant effect of Chinese herbal volatile oils and can remove active oxygen, thereby effectively inhibiting articular cartilage damage caused by oxidative stress.

[0021] Third, the present invention obtains joint bionic synovial fluid with excellent comprehensive performance through comprehensive investigation of lubrication performance and anti-oxidation performance, which has important application prospects in the field of biological lubrication.

[0022] Fourth, the antioxidant properties of the Pickering breast joint bionic synovial fluid, a Chinese medicine volatile oil of the present invention: DPPH free radicals were investigated, and the scavenging ability of the joint bionic synovial fluid at a concentration of 0.5 to 8 mg / mL on DPPH free radicals was determined. It was found that the joint bionic synovial fluid had good antioxidant properties, and the antioxidant activity gradually increased with the increase of concentration.

[0023] Fifth, the tribological properties of the Pickering breast joint bionic synovial fluid of the Chinese medicine volatile oil of the present invention: titanium alloy balls (φ6mm) and titanium alloy discs (28×28×2.65mm) were used to evaluate the tribological properties of the joint bionic synovial fluid under the friction test conditions of 0.5N load, 60-180r / min rotation speed, 0.5h test time, and 5mm friction test stroke. It was found that the joint bionic synovial fluid had good tribological properties, and the friction coefficient of the joint bionic synovial fluid at each rotation speed was significantly lower than that of pure water.

[0024] Other features and advantages of the present invention will be described in the following description, and partly become apparent from the description, or understood by practicing the present invention. The purpose and other advantages of the present invention can be realized and obtained by the structures pointed out in the description, claims and drawings. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] Figure 1 A flow chart of a method for preparing Pickering breast joint bionic synovial fluid of a traditional Chinese medicine volatile oil according to an exemplary embodiment of the present invention;

[0026] Figure 2 The appearance pictures and optical microscope comparison pictures of Pickering breast joint bionic synovial fluid obtained by exemplary embodiments 1 to 4 of the present invention;

[0027] Figure 3 A schematic diagram of the placement stability of the Pickering breast joint bionic synovial fluid of the Chinese medicine volatile oil obtained in exemplary embodiment 5 of the present invention;

[0028] Figure 4 This is a graph showing the DPPH free radical scavenging activity of Pickering breast joint bionic synovial fluid, a volatile oil of traditional Chinese medicine, obtained in exemplary embodiment 6 of the present invention;

[0029] Figure 5 This is a graph showing the friction reduction performance of Pickering breast joint bionic synovial fluid of traditional Chinese medicine volatile oil obtained in exemplary embodiment 7 of the present invention. DETAILED DESCRIPTION

[0030] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0031] In order to solve the deficiencies of the prior art, the present invention discloses a method for preparing Pickering breast joint bionic synovial fluid of Chinese medicine volatile oil. Figure 1 The flowchart of the preparation method of Pickering breast joint bionic synovial fluid of a traditional Chinese medicine volatile oil of an exemplary embodiment of the present invention is as follows: Figure 1 As shown, the method comprises the following steps:

[0032] Step S1, adding nanoparticles into ultrapure water, and ultrasonically dispersing to obtain a nanoparticle dispersion;

[0033] Step S2, adding Chinese herbal volatile oil into the nanoparticle dispersion according to a certain volume fraction, and ultrasonically stirring to obtain Chinese herbal volatile oil Pickering emulsion joint biomimetic synovial fluid.

[0034] In step S1 of the present invention, the nanoparticles are one or a combination of graphene oxide, fullerene, nanodiamond, carbon nanotube, nanosilicon dioxide and nanocellulose, preferably graphene oxide; wherein the mass ratio of oxygen-containing functional groups on the surface of the nanoparticles is 28% to 37%.

[0035] The conditions for the ultrasonic dispersion are: ultrasonic power is 150 to 1000 W, preferably 450 W, and ultrasonic dispersion time is 1 to 2 hours.

[0036] The concentration of the nanoparticle dispersion is 2.0-6.0 g / L.

[0037] In step S2 of the present invention, the certain volume fraction is 10% to 20%.

[0038] The Chinese medicine volatile oil is one or a combination of angelica volatile oil, chuanxiong rhizome volatile oil, pubescent volatile oil, cassia twig volatile oil, salvia miltiorrhiza volatile oil, eucommia ulmoides volatile oil, white peony root volatile oil, wormwood volatile oil, notopterygium root volatile oil, Achyranthes bidentata volatile oil, asarum volatile oil, qiannianjian volatile oil, turmeric volatile oil and houttuynia cordata volatile oil, preferably angelica volatile oil.

[0039] The conditions for ultrasonic stirring are: ultrasonic power of 50-500 W, preferably 450 W, ultrasonic temperature of 30-70° C., and ultrasonic stirring time of 0.05-1 h, preferably 10 min.

[0040] Embodiment 1:

[0041] Exemplary embodiment 1 of the present invention provides a method for preparing Pickering breast joint bionic synovial fluid of Chinese medicine volatile oil, and the specific steps are as follows:

[0042] Step S1, adding a graphene oxide sample to ultrapure water, wherein the mass ratio of the surface oxygen-containing functional groups of the graphene oxide is 28% to 37%, and ultrasonically dispersing the sample at an ultrasonic power of 450 W for 1 h to obtain a graphene oxide dispersion having a concentration of 2.0 g / L;

[0043] Step S2, adding angelica volatile oil to the graphene oxide dispersion at a volume fraction of 20%, placing it under ultrasonic stirring for 10 minutes, ultrasonic power of 450W, and ultrasonic temperature of 30°C to obtain the Chinese medicine volatile oil Pickering breast joint bionic synovial fluid.

[0044] The Pickering breast joint bionic synovial fluid of the Chinese medicine volatile oil prepared in Example 1 of the present invention was left to stand for 72 hours at room temperature and then photographed digitally and under a microscope. Figure 2 As shown in a.

[0045] Embodiment 2:

[0046] Exemplary embodiment 2 of the present invention provides a method for preparing Pickering breast joint bionic synovial fluid of Chinese medicine volatile oil, and the specific steps are as follows:

[0047] Step S1, adding a graphene oxide sample to ultrapure water, wherein the mass ratio of the surface oxygen-containing functional groups of the graphene oxide is 28% to 37%, and ultrasonically dispersing the sample at an ultrasonic power of 450 W for 1 h to obtain a graphene oxide dispersion having a concentration of 2.0 g / L;

[0048] Step S2, adding angelica volatile oil to the graphene oxide dispersion at a volume fraction of 20%, placing it under ultrasonic stirring for 10 minutes, ultrasonic power of 450W, and ultrasonic temperature of 70°C to obtain the Chinese medicine volatile oil Pickering breast joint bionic synovial fluid.

[0049] The Pickering breast joint bionic synovial fluid of the Chinese medicine volatile oil prepared in Example 2 of the present invention was left to stand for 72 hours at room temperature and then photographed digitally and under a microscope. Figure 2 As shown in b.

[0050] Embodiment 3:

[0051] Exemplary embodiment 3 of the present invention provides a method for preparing Pickering breast joint bionic synovial fluid of Chinese medicine volatile oil, and the specific steps are as follows:

[0052] Step S1, adding a graphene oxide sample to ultrapure water, wherein the mass ratio of the surface oxygen-containing functional groups of the graphene oxide is 28% to 37%, and ultrasonically dispersing the sample at an ultrasonic power of 450 W for 1 h to obtain a graphene oxide dispersion having a concentration of 2.0 g / L;

[0053] Step S2, adding angelica volatile oil to the graphene oxide dispersion at a volume fraction of 10%, placing it under ultrasonic stirring for 10 minutes, ultrasonic power of 450W, and ultrasonic temperature of 50°C to obtain the Chinese medicine volatile oil Pickering breast joint bionic synovial fluid.

[0054] The Pickering breast joint bionic synovial fluid of the Chinese medicine volatile oil prepared in exemplary embodiment 3 of the present invention was left to stand for 72 hours at room temperature and then photographed digitally and under a microscope. Figure 2 As shown in c.

[0055] Embodiment 4:

[0056] Exemplary embodiment 4 of the present invention provides a method for preparing Pickering breast joint bionic synovial fluid of Chinese medicine volatile oil, and the specific steps are as follows:

[0057] Step S1, adding a graphene oxide sample to ultrapure water, wherein the mass ratio of the surface oxygen-containing functional groups of the graphene oxide is 28% to 37%, and ultrasonically dispersing the sample at an ultrasonic power of 450 W for 2 hours to obtain a graphene oxide dispersion having a concentration of 4.0 g / L;

[0058] Step S2, adding angelica volatile oil to the graphene oxide dispersion at a volume fraction of 20%, placing it under ultrasonic stirring for 10 minutes, ultrasonic power of 450W, and ultrasonic temperature of 50°C to obtain the Chinese medicine volatile oil Pickering breast joint bionic synovial fluid.

[0059] The Pickering breast joint bionic synovial fluid of the Chinese medicine volatile oil prepared by the exemplary embodiment 4 of the present invention was left to stand for 72 hours at room temperature and then photographed digitally and under a microscope. Figure 2 As shown in middle d.

[0060] Embodiment 5:

[0061] Exemplary embodiment 5 of the present invention provides a method for preparing Pickering breast joint bionic synovial fluid of Chinese medicine volatile oil, and the specific steps are as follows:

[0062] Step S1, adding a graphene oxide sample to ultrapure water, wherein the mass ratio of the surface oxygen-containing functional groups of the graphene oxide is 28% to 37%, and ultrasonically dispersing the sample at an ultrasonic power of 450 W for 2 h to obtain a graphene oxide dispersion having a concentration of 6.0 g / L;

[0063] Step S2, adding angelica volatile oil to the graphene oxide dispersion at a volume fraction of 20%, placing it under ultrasonic stirring for 10 minutes, ultrasonic power of 450W, and ultrasonic temperature of 50°C to obtain the Chinese medicine volatile oil Pickering breast joint bionic synovial fluid.

[0064] The Pickering breast joint bionic synovial fluid, a Chinese herbal volatile oil prepared in Example 5 of the present invention, was stored at room temperature for 30 days and photographed digitally and under a microscope. Figure 3 Schematic diagram of the placement stability of Pickering breast joint bionic synovial fluid of volatile oil of traditional Chinese medicine obtained in exemplary embodiment 5 of the present invention, as shown in Figure 3 As shown, within 30 days of placement, the appearance of the emulsion and the state of the emulsion droplets did not change significantly, indicating that the prepared emulsion has good stability.

[0065] Embodiment 6:

[0066] Exemplary embodiment 6 of the present invention provides an evaluation of the antioxidant properties of Pickering's bionic synovial fluid, a volatile oil of traditional Chinese medicine, and the specific steps are as follows:

[0067] Step S1, adding a graphene oxide sample to ultrapure water, wherein the mass ratio of the surface oxygen-containing functional groups of the graphene oxide is 28% to 37%, and ultrasonically dispersing the sample at an ultrasonic power of 450 W for 2 h to obtain a graphene oxide dispersion having a concentration of 6.0 g / L;

[0068] Step S2, adding angelica volatile oil to the graphene oxide dispersion at a volume fraction of 20%, placing it under ultrasonic stirring for 10 minutes, ultrasonic power of 450W, and ultrasonic temperature of 50°C to obtain the Chinese medicine volatile oil Pickering breast joint bionic synovial fluid.

[0069] The Pickering breast joint bionic synovial fluid, a Chinese herbal volatile oil prepared in exemplary embodiment 6 of the present invention, was configured into joint bionic synovial fluids with concentrations of 0.5 mg / mL, 1 mg / mL, 2 mg / mL, 4 mg / mL, and 8 mg / mL, respectively. DPPH free radicals were taken as the object of investigation, and their scavenging ability for DPPH free radicals was measured. Figure 4 This is a graph showing the DPPH free radical scavenging activity of Pickering breast joint bionic synovial fluid of volatile oil of traditional Chinese medicine obtained in exemplary embodiment 6 of the present invention, as shown in FIG. Figure 4 As shown, the bionic synovial fluid has good antioxidant properties, and with the increase of concentration, the antioxidant activity gradually increases. When the concentration is 8 mg / mL, the scavenging rate of bionic synovial fluid on DPPH free radicals reaches 80.9%.

[0070] Embodiment 7:

[0071] Exemplary embodiment 7 of the present invention provides a tribological performance evaluation of Pickering breast joint bionic synovial fluid of a Chinese medicine volatile oil, and the specific steps are as follows:

[0072] Step S1, adding a graphene oxide sample to ultrapure water, wherein the mass ratio of the surface oxygen-containing functional groups of the graphene oxide is 28% to 37%, and ultrasonically dispersing the sample at an ultrasonic power of 450 W for 2 h to obtain a graphene oxide dispersion having a concentration of 6.0 g / L;

[0073] Step S2, adding angelica volatile oil to the graphene oxide dispersion at a volume fraction of 20%, placing it under ultrasonic stirring at an ultrasonic time of 10 minutes, an ultrasonic power of 450W, and an ultrasonic temperature of 50°C to obtain the Chinese medicine volatile oil Pickering breast joint bionic synovial fluid.

[0074] The Pickering breast joint bionic synovial fluid, a Chinese herbal volatile oil prepared in exemplary embodiment 7 of the invention, was used to evaluate the tribological properties of the joint bionic synovial fluid under the friction test conditions of a load of 0.5N, a test time of 0.5h, a friction test stroke of 5mm, and a rotation speed of 60r / min, 120r / min, and 180r / min. Figure 5 This is a friction reduction performance diagram of Pickering breast joint bionic synovial fluid of Chinese medicine volatile oil obtained in exemplary embodiment 7 of the present invention, as shown in Figure 5 As shown in the figure, under the speed conditions of 60r / min, 120r / min, and 180r / min, the average friction coefficients of joint bionic synovial fluid are 0.307, 0.273, and 0.21, respectively, and the average friction coefficients of pure water are 0.44, 0.377, and 0.357, respectively. The results show that the average friction coefficient of joint bionic synovial fluid is significantly lower than that of pure water at each speed. Under the speed conditions of 60r / min, 120r / min, and 180r / min, the average friction coefficient of joint bionic synovial fluid is 30%, 28%, and 41% lower than that of water, respectively, reflecting that joint bionic synovial fluid has good tribological properties.

[0075] Finally, it should be noted that the above is only a preferred embodiment of the present invention and is not intended to limit the present invention. Although the present invention has been described in detail with reference to the aforementioned embodiments, it is still possible for those skilled in the art to modify the technical solutions described in the aforementioned embodiments or to make equivalent substitutions for some of the technical features therein. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

Claims

1. A method for preparing Pickering breast joint bionic synovial fluid of Chinese medicine volatile oil, characterized in that: The method comprises the following steps: Adding nanoparticles to ultrapure water and dispersing by ultrasonication to obtain a nanoparticle dispersion; the nanoparticles are graphene oxide, and the mass ratio of oxygen-containing functional groups on the surface of the nanoparticles is 28% to 37%; The conditions of the ultrasonic dispersion are: ultrasonic power of 150 to 1000 W, and ultrasonic dispersion time of 1 to 2 h; Adding Chinese herbal volatile oil to the nanoparticle dispersion at a certain volume fraction, and ultrasonically stirring to obtain Chinese herbal volatile oil Pickering emulsion joint biomimetic synovial fluid; the Chinese herbal volatile oil is angelica volatile oil; The conditions of the ultrasonic stirring are: The ultrasonic power is 50-500W, the ultrasonic temperature is 30-70°C, and the ultrasonic stirring time is 0.05-1h.

2. The method for preparing the Pickering breast joint bionic synovial fluid of a Chinese medicine volatile oil according to claim 1, characterized in that: The concentration of the nanoparticle dispersion is 2.0-6.0 g / L.

3. The method for preparing the Pickering breast joint bionic synovial fluid of a Chinese medicine volatile oil according to claim 1, characterized in that: The certain volume fraction is 10% to 20%.

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