A sediment purifier for in-situ degradation of river and lake bottom silt

Abstract

The present invention discloses a sludge purifier for in-situ degradation of sludge at the bottom of rivers and lakes, belonging to the field of environmental protection technology. The sludge purifier comprises the following components, measured in parts by weight: volcanic rock, PAC, biological strains, and a sludge solidifying agent, wherein the biological strains include Pseudomonas aeruginosa, Bacillus subtilis, and Aspergillus niger; and the sludge solidifying agent includes polyacrylamide, anhydrous sodium sulfate, magnesium oxide, and calcium oxide. The preparation method is as follows: (1) washing the volcanic rock and PAC and using them as carriers; (2) dispersing the carriers in a culture medium, adding the selected biological strains to the culture medium for expansion and cultivation, and centrifuging to obtain a carrier with in-situ growth of bacteria on the surface; (3) adding the carrier with in-situ growth of bacteria on the surface to the sludge solidifying agent and continuously stirring, and then standing for a period of time to obtain the sludge solidifying agent. The sludge solidifying agent can effectively degrade pollutants in sludge at the bottom of rivers and lakes.

Description

Technical Field

[0001] The invention belongs to the technical field of environmental protection, and in particular relates to a bottom mud purifier for in-situ degradation of silt at the bottom of rivers and lakes. Background Art

[0002] Sediment purifier is a biological product made by mixing sediment-degrading microbial strains with selected minerals. It can amplify the natural sediment degradation effect hundreds of times, effectively decompose odorous substances, and quickly change the water body from an anaerobic state to an aerobic state. It is a technology and product that effectively degrades sediment, helps water bodies restore their self-purification ability, and rebuild their ecological balance.

[0003] The role of sediment purifiers is mainly reflected in two aspects: one is to decompose and oxidize pollutants in the sediment, reduce the thickness of the sediment, and reduce the pollution impact on the overlying water body; the other is to passivate the sediment and reduce its activity, forming a protective layer on the surface of the sediment, and controlling the release of heavy metal pollutants in the sediment.

[0004] The main application form of sediment decomposition and oxidation is in situ bioremediation, that is, the contaminated sediment is not handled or transported, and sediment purifiers are directly added to the sediment to degrade and repair it at the bottom of the water body without basically destroying the natural environment of the sediment.

[0005] Chinese patent application number: CN201811358478.6, publication number: CN109928509A, discloses a black and smelly river sediment purifier and its preparation method. The technical solution is as follows:

[0006] "The black and odorous river sediment purifier uses a mixed porous material as a carrier, on which fungi are grown in situ and embedded and fixed by auxiliary agents. On the one hand, the mixed porous material of the carrier can effectively adsorb floating particulate pollutants. Compared with a single porous material, the mixed porous material has different pore sizes and can adsorb more floating particulate pollutants. On the other hand, the high specific surface area of ​​the mixed porous material is very beneficial for its use as a carrier for the growth of fungi, giving the fungi a larger growth space. The fungi can effectively degrade and absorb nitrogen, phosphorus, and sulfur elements in water bodies and black and odorous river sediments, as well as difficult-to-decompose organic pollutants, thereby significantly purifying water bodies and black and odorous river sediments and eliminating odors. By embedding and fixing the fungi with auxiliary agents without hindering the overall purifier effect, the fungi are not easy to fall off and cause secondary pollution, which is conducive to recovery. It can also protect the fungi growing on the carrier, reduce the influence of factors such as the acid-base environment of the water body or the black and smelly river mud on the fungi, improve their stability and expand their scope of application. Preferably, the mixed porous material is at least two of zeolite, bentonite and fly ash. Zeolite, bentonite and fly ash are all common porous materials, and bentonite is preferably calcareous bentonite, which has the advantages of wide sources, low cost and high cost performance. Preferably, the fungi grown on the carrier are denitrifying thiobacillus, EM bacteria and nitrifying bacteria. Denitrifying thiobacillus can absorb nitrogen and sulfur elements. Absorption, nitrifying bacteria can absorb and adsorb ammonia nitrogen pollutants, EM bacteria itself is a mixed bacteria, which has a better absorption and adsorption effect on nitrogen and phosphorus elements, and the above-mentioned fungi can all absorb and degrade organic pollutants. Preferably, the auxiliary agent includes sodium alginate, calcium nitrate, aluminum sulfate and calcium chloride. Sodium alginate can ensure that fungi adsorb and enrich pollutants while protecting fungi to avoid being affected by toxic substances or acid-base environment, and under the joint action of calcium nitrate, aluminum sulfate and calcium chloride, its embedding and fixing effect is more excellent. The three inorganic salt components form a skeleton to improve the stability of embedding and fixing. A preparation method for a black and smelly river sediment purifier, the preparation method comprises the following preparation steps: 1) mixing the mixed The porous material is washed and used as a carrier for later use; 2) the carrier is dispersed in the culture medium, the selected bacteria are added to the culture medium for expansion and cultivation, and the carrier with in situ surface growth fungi is obtained by centrifugation; 3) the auxiliary agent is prepared into a modification liquid with a total concentration of ≥15wt%, the carrier with in situ surface growth fungi is placed in the modification liquid and stirred, and then the black and smelly river sediment purifier is obtained after standing for a period of time. The overall preparation method is simple and efficient, and the purifier can be prepared quickly and in batches. Preferably, the culture medium in step 2) is Knop culture medium. Knop culture medium is a common culture medium with the advantages of low cost and easy preparation. Preferably, the stirring process in step 3) is carried out by slow stirring at a speed of 20 to 35 rpm for at least 15 min.Stirring slowly prevents the fungi from falling off and allows the carrier with the fungi growing in situ on the surface to evenly contact the modified liquid. Preferably, the standing time in step 3) is 1 to 3 hours. Summary of the Invention

[0007] 1. Problems to be solved

[0008] In view of the problems existing in the above-mentioned prior art, the present invention provides a sediment purifier for in-situ degradation of river and lake bottom sludge, which can effectively degrade pollutants in river and lake bottom sludge.

[0009] 2. Technical solution

[0010] To solve the above problems, the present invention adopts the following technical solutions.

[0011] A sediment purifier for in-situ degradation of silt at the bottom of rivers and lakes.

[0012] In parts by weight, it comprises the following components:

[0013]

[0014] The biological strains include Pseudomonas aeruginosa, Bacillus subtilis and Aspergillus niger, and the mass ratio of the Pseudomonas aeruginosa, the Bacillus subtilis and the Aspergillus niger is 1:2:5;

[0015] The sludge solidifying agent includes polyacrylamide, anhydrous sodium sulfate, magnesium oxide and calcium oxide, and the mass ratio of the polyacrylamide, the anhydrous sodium sulfate, the magnesium oxide and the calcium oxide is 1:5:2:1.

[0016] In the above-mentioned bottom mud purifier for in-situ degradation of river and lake bottom mud,

[0017] In parts by weight, it comprises the following components:

[0018]

[0019]

[0020] The biological strains include Pseudomonas aeruginosa, Bacillus subtilis and Aspergillus niger, and the mass ratio of the Pseudomonas aeruginosa, the Bacillus subtilis and the Aspergillus niger is 1:2:5;

[0021] The sludge solidifying agent includes polyacrylamide, anhydrous sodium sulfate, magnesium oxide and calcium oxide, and the mass ratio of the polyacrylamide, the anhydrous sodium sulfate, the magnesium oxide and the calcium oxide is 1:5:2:1.

[0022] In the above-mentioned bottom mud purifier for in-situ degradation of river and lake bottom mud,

[0023] In parts by weight, it comprises the following components:

[0024]

[0025] The biological strains include Pseudomonas aeruginosa, Bacillus subtilis and Aspergillus niger, and the mass ratio of the Pseudomonas aeruginosa, the Bacillus subtilis and the Aspergillus niger is 1:2:5;

[0026] The sludge solidifying agent includes polyacrylamide, anhydrous sodium sulfate, magnesium oxide and calcium oxide, and the mass ratio of the polyacrylamide, the anhydrous sodium sulfate, the magnesium oxide and the calcium oxide is 1:5:2:1.

[0027] In the above-mentioned bottom mud purifier for in-situ degradation of river and lake bottom mud,

[0028] The Pseudomonas aeruginosa, the Bacillus subtilis and the Aspergillus niger were all purchased from Shanghai Chuangjing Bio-Environmental Protection Technology Co., Ltd.

[0029] In the above-mentioned sediment purifier for in-situ degradation of river and lake bottom silt,

[0030] The particle size of the volcanic rock is in the range of 3-6 mm;

[0031] The volcanic stone is black or red volcanic stone.

[0032] In the above-mentioned sediment purifier for in-situ degradation of river and lake bottom silt,

[0033] The PAC is industrial-grade black or yellow polyaluminium chloride, with a mass content of 28%.

[0034] In the above-mentioned sediment purifier for in-situ degradation of river and lake bottom silt,

[0035] The total colony count of the biological strain is ≥30 cfu / g.

[0036] In the above-mentioned bottom mud purifier for in-situ degradation of river and lake bottom mud,

[0037] The sludge solidifying agent is a gray powder;

[0038] The particle size range of the sludge solidifying agent is 100 mesh to 200 mesh;

[0039] The mass fraction of insoluble matter in the sludge solidifying agent is ≤1.3%.

[0040] In the above-mentioned bottom mud purifier for in-situ degradation of river and lake bottom mud,

[0041] The preparation method of the sediment purifier is as follows:

[0042] (1) Wash the volcanic rock and PAC and use them as carriers;

[0043] (2) dispersing the carrier in a culture medium, adding the selected biological strain into the culture medium for expansion and cultivation, and centrifuging to obtain the carrier with in situ bacterial growth on its surface;

[0044] (3) Add the carrier of the in-situ growing bacteria on the surface to the sludge solidifying agent and continue stirring, and then let it stand for a period of time to obtain the product.

[0045] In the above-mentioned bottom mud purifier for in-situ degradation of river and lake bottom mud,

[0046] The culture medium described in step (2) is LB culture medium.

[0047] 3. Beneficial effects

[0048] Compared with the prior art, the present invention has the following beneficial effects:

[0049] (1) The prepared sediment purifier has a fast effect and can quickly eliminate the black and smelly sediment, increase the concentration of microorganisms in the polluted medium, promote the rapid repair of water organisms, and improve the self-purification capacity;

[0050] (2) The prepared sediment purifier increases the dissolved oxygen (DO) in the water body, and can restore the water body from black and smelly anaerobic to a dissolved oxygen level of more than 2 to 4 mg / L;

[0051] (3) The prepared sediment purifier can eliminate the internal pollution (silt) of the water body, the silt will not rise, there is no odor, and the silt can be gradually mineralized into a dense earthy brown color, reducing the thickness of the silt layer;

[0052] (4) The prepared sediment purifier has a wide range of applications. It can be used to degrade sediment in urban polluted rivers (black and smelly water bodies) to reduce the thickness of the sediment. It can also be used to decompose and reduce the sediment in polluted lakes to improve the bottom ecosystem. BRIEF DESCRIPTION OF THE DRAWINGS

[0053] Figure 1 This is a diagram showing the degree of blackness and odor in the river before the sediment purifier prepared in Example 5 of the present invention is added;

[0054] Figure 2 This is a diagram showing the effect of the sediment purifier prepared in Example 5 of the present invention three months after being put into use;

[0055] Figure 3 This is an actual product picture of the sediment purifier prepared in Example 5 of the present invention;

[0056] Figure 4 This is a diagram showing the degree of black and smelly river sediment before the sediment purifier prepared in Example 5 of the present invention is added;

[0057] Figure 5 This is a picture showing the effect of the black and smelly river sediment after the sediment purifier prepared in Example 5 of the present invention is added;

[0058] Figure 6 This is a graph showing the test results of the sediment purifier prepared in Example 5 of the present invention. DETAILED DESCRIPTION

[0059] The present invention will be further described below with reference to specific embodiments.

[0060] Example 1

[0061] The bottom mud purifier of this embodiment is used for in-situ degradation of river and lake bottom mud.

[0062] In parts by weight, it comprises the following components:

[0063]

[0064] The biological strains include Pseudomonas aeruginosa, Bacillus subtilis and Aspergillus niger, and the mass ratio of the Pseudomonas aeruginosa, the Bacillus subtilis and the Aspergillus niger is 1:2:5;

[0065] The sludge solidifying agent includes polyacrylamide, anhydrous sodium sulfate, magnesium oxide and calcium oxide, and the mass ratio of the polyacrylamide, the anhydrous sodium sulfate, the magnesium oxide and the calcium oxide is 1:5:2:1.

[0066] In the above-mentioned bottom mud purifier for in-situ degradation of river and lake bottom mud,

[0067] The Pseudomonas aeruginosa, the Bacillus subtilis and the Aspergillus niger were all purchased from Shanghai Chuangjing Bio-Environmental Protection Technology Co., Ltd.

[0068] In the above-mentioned bottom mud purifier for in-situ degradation of river and lake bottom mud,

[0069] The particle size of the volcanic rock is in the range of 3-6 mm;

[0070] The volcanic stone is black or red volcanic stone.

[0071] In the above-mentioned bottom mud purifier for in-situ degradation of river and lake bottom mud,

[0072] The PAC is industrial-grade black or yellow polyaluminium chloride, with a mass content of 28%.

[0073] In the above-mentioned bottom mud purifier for in-situ degradation of river and lake bottom mud,

[0074] The total colony count of the biological strain is ≥30 cfu / g.

[0075] In the above-mentioned bottom mud purifier for in-situ degradation of river and lake bottom mud,

[0076] The sludge solidifying agent is a gray powder;

[0077] The particle size range of the sludge solidifying agent is 100 mesh to 200 mesh;

[0078] The mass fraction of insoluble matter in the sludge solidifying agent is ≤1.3%.

[0079] In the above-mentioned bottom mud purifier for in-situ degradation of river and lake bottom mud,

[0080] The preparation method of the sediment purifier is as follows:

[0081] (1) Wash the volcanic rock and PAC and use them as carriers;

[0082] (2) dispersing the carrier in a culture medium, adding the selected biological strain into the culture medium for expansion and cultivation, and centrifuging to obtain the carrier with in situ bacterial growth on its surface;

[0083] (3) Add the carrier of the in-situ growing bacteria on the surface to the sludge solidifying agent and continue stirring, and then let it stand for a period of time to obtain the product.

[0084] In the above-mentioned bottom mud purifier for in-situ degradation of river and lake bottom mud,

[0085] The culture medium described in step (2) is LB culture medium.

[0086] Example 2

[0087] The bottom mud purifier of this embodiment is used for in-situ degradation of river and lake bottom mud.

[0088] In parts by weight, it comprises the following components:

[0089]

[0090] The biological strains include Pseudomonas aeruginosa, Bacillus subtilis and Aspergillus niger, and the mass ratio of the Pseudomonas aeruginosa, the Bacillus subtilis and the Aspergillus niger is 1:2:5;

[0091] The sludge solidifying agent includes polyacrylamide, anhydrous sodium sulfate, magnesium oxide and calcium oxide, and the mass ratio of the polyacrylamide, the anhydrous sodium sulfate, the magnesium oxide and the calcium oxide is 1:5:2:1.

[0092] In the above-mentioned bottom mud purifier for in-situ degradation of river and lake bottom mud,

[0093] The Pseudomonas aeruginosa, the Bacillus subtilis and the Aspergillus niger were all purchased from Shanghai Chuangjing Bio-Environmental Protection Technology Co., Ltd.

[0094] In the above-mentioned bottom mud purifier for in-situ degradation of river and lake bottom mud,

[0095] The particle size of the volcanic rock is in the range of 3-6 mm;

[0096] The volcanic stone is black or red volcanic stone.

[0097] In the above-mentioned bottom mud purifier for in-situ degradation of river and lake bottom mud,

[0098] The PAC is industrial-grade black or yellow polyaluminium chloride, with a mass content of 28%.

[0099] In the above-mentioned bottom mud purifier for in-situ degradation of river and lake bottom mud,

[0100] The total colony count of the biological strain is ≥30 cfu / g.

[0101] In the above-mentioned bottom mud purifier for in-situ degradation of river and lake bottom mud,

[0102] The sludge solidifying agent is a gray powder;

[0103] The particle size range of the sludge solidifying agent is 100 mesh to 200 mesh;

[0104] The mass fraction of insoluble matter in the sludge solidifying agent is ≤1.3%.

[0105] In the above-mentioned bottom mud purifier for in-situ degradation of river and lake bottom mud,

[0106] The preparation method of the sediment purifier is as follows:

[0107] (1) Wash the volcanic rock and PAC and use them as carriers;

[0108] (2) dispersing the carrier in a culture medium, adding the selected biological strain into the culture medium for expansion and cultivation, and centrifuging to obtain the carrier with in situ bacterial growth on its surface;

[0109] (3) Add the carrier of the in-situ growing bacteria on the surface to the sludge solidifying agent and continue stirring, and then let it stand for a period of time to obtain the product.

[0110] In the above-mentioned bottom mud purifier for in-situ degradation of river and lake bottom mud,

[0111] The culture medium described in step (2) is LB culture medium.

[0112] Example 3

[0113] The bottom mud purifier of this embodiment is used for in-situ degradation of river and lake bottom mud.

[0114] In parts by weight, it comprises the following components:

[0115]

[0116] The biological strains include Pseudomonas aeruginosa, Bacillus subtilis and Aspergillus niger, and the mass ratio of the Pseudomonas aeruginosa, the Bacillus subtilis and the Aspergillus niger is 1:2:5;

[0117] The sludge solidifying agent includes polyacrylamide, anhydrous sodium sulfate, magnesium oxide and calcium oxide, and the mass ratio of the polyacrylamide, the anhydrous sodium sulfate, the magnesium oxide and the calcium oxide is 1:5:2:1.

[0118] In the above-mentioned bottom mud purifier for in-situ degradation of river and lake bottom mud,

[0119] The Pseudomonas aeruginosa, the Bacillus subtilis and the Aspergillus niger were all purchased from Shanghai Chuangjing Bio-Environmental Protection Technology Co., Ltd.

[0120] In the above-mentioned bottom mud purifier for in-situ degradation of river and lake bottom mud,

[0121] The particle size of the volcanic rock is in the range of 3-6 mm;

[0122] The volcanic stone is black or red volcanic stone.

[0123] In the above-mentioned bottom mud purifier for in-situ degradation of river and lake bottom mud,

[0124] The PAC is industrial-grade black or yellow polyaluminium chloride, with a mass content of 28%.

[0125] In the above-mentioned bottom mud purifier for in-situ degradation of river and lake bottom mud,

[0126] The total colony count of the biological strain is ≥30 cfu / g.

[0127] In the above-mentioned bottom mud purifier for in-situ degradation of river and lake bottom mud,

[0128] The sludge solidifying agent is a gray powder;

[0129] The particle size range of the sludge solidifying agent is 100 mesh to 200 mesh;

[0130] The mass fraction of insoluble matter in the sludge solidifying agent is ≤1.3%.

[0131] In the above-mentioned bottom mud purifier for in-situ degradation of river and lake bottom mud,

[0132] The preparation method of the sediment purifier is as follows:

[0133] (1) Wash the volcanic rock and PAC and use them as carriers;

[0134] (2) dispersing the carrier in a culture medium, adding the selected biological strain into the culture medium for expansion and cultivation, and centrifuging to obtain the carrier with in situ bacterial growth on its surface;

[0135] (3) Add the carrier of the in-situ growing bacteria on the surface to the sludge solidifying agent and continue stirring, and then let it stand for a period of time to obtain the product.

[0136] In the above-mentioned bottom mud purifier for in-situ degradation of river and lake bottom mud,

[0137] The culture medium described in step (2) is LB culture medium.

[0138] Example 4

[0139] The bottom mud purifier of this embodiment is used for in-situ degradation of river and lake bottom mud.

[0140] In parts by weight, it comprises the following components:

[0141]

[0142] The biological strains include Pseudomonas aeruginosa, Bacillus subtilis and Aspergillus niger, and the mass ratio of the Pseudomonas aeruginosa, the Bacillus subtilis and the Aspergillus niger is 1:2:5;

[0143] The sludge solidifying agent includes polyacrylamide, anhydrous sodium sulfate, magnesium oxide and calcium oxide, and the mass ratio of the polyacrylamide, the anhydrous sodium sulfate, the magnesium oxide and the calcium oxide is 1:5:2:1.

[0144] In the above-mentioned bottom mud purifier for in-situ degradation of river and lake bottom mud,

[0145] The Pseudomonas aeruginosa, the Bacillus subtilis and the Aspergillus niger were all purchased from Shanghai Chuangjing Bio-Environmental Protection Technology Co., Ltd.

[0146] In the above-mentioned bottom mud purifier for in-situ degradation of river and lake bottom mud,

[0147] The particle size of the volcanic rock is in the range of 3-6 mm;

[0148] The volcanic stone is black or red volcanic stone.

[0149] In the above-mentioned bottom mud purifier for in-situ degradation of river and lake bottom mud,

[0150] The PAC is industrial-grade black or yellow polyaluminium chloride, with a mass content of 28%.

[0151] In the above-mentioned bottom mud purifier for in-situ degradation of river and lake bottom mud,

[0152] The total colony count of the biological strain is ≥30 cfu / g.

[0153] In the above-mentioned bottom mud purifier for in-situ degradation of river and lake bottom mud,

[0154] The sludge solidifying agent is a gray powder;

[0155] The particle size range of the sludge solidifying agent is 100 mesh to 200 mesh;

[0156] The mass fraction of insoluble matter in the sludge solidifying agent is ≤1.3%.

[0157] In the above-mentioned bottom mud purifier for in-situ degradation of river and lake bottom mud,

[0158] The preparation method of the sediment purifier is as follows:

[0159] (1) Wash the volcanic rock and PAC and use them as carriers;

[0160] (2) dispersing the carrier in a culture medium, adding the selected biological strain into the culture medium for expansion and cultivation, and centrifuging to obtain the carrier with in situ bacterial growth on its surface;

[0161] (3) Add the carrier of the in-situ growing bacteria on the surface to the sludge solidifying agent and continue stirring, and then let it stand for a period of time to obtain the product.

[0162] In the above-mentioned bottom mud purifier for in-situ degradation of river and lake bottom mud,

[0163] The culture medium described in step (2) is LB culture medium.

[0164] Example 5

[0165] The bottom mud purifier of this embodiment is used for in-situ degradation of river and lake bottom mud.

[0166] In parts by weight, it comprises the following components:

[0167]

[0168] The biological strains include Pseudomonas aeruginosa, Bacillus subtilis and Aspergillus niger, and the mass ratio of the Pseudomonas aeruginosa, the Bacillus subtilis and the Aspergillus niger is 1:2:5;

[0169] The sludge solidifying agent includes polyacrylamide, anhydrous sodium sulfate, magnesium oxide and calcium oxide, and the mass ratio of the polyacrylamide, the anhydrous sodium sulfate, the magnesium oxide and the calcium oxide is 1:5:2:1.

[0170] In the above-mentioned bottom mud purifier for in-situ degradation of river and lake bottom mud,

[0171] The Pseudomonas aeruginosa, the Bacillus subtilis and the Aspergillus niger were all purchased from Shanghai Chuangjing Bio-Environmental Protection Technology Co., Ltd.

[0172] In the above-mentioned bottom mud purifier for in-situ degradation of river and lake bottom mud,

[0173] The particle size of the volcanic rock is in the range of 3-6 mm;

[0174] The volcanic stone is black or red volcanic stone.

[0175] In the above-mentioned bottom mud purifier for in-situ degradation of river and lake bottom mud,

[0176] The PAC is industrial-grade black or yellow polyaluminium chloride, with a mass content of 28%.

[0177] In the above-mentioned bottom mud purifier for in-situ degradation of river and lake bottom mud,

[0178] The total colony count of the biological strain is ≥30 cfu / g.

[0179] In the above-mentioned bottom mud purifier for in-situ degradation of river and lake bottom mud,

[0180] The sludge solidifying agent is a gray powder;

[0181] The particle size range of the sludge solidifying agent is 100 mesh to 200 mesh;

[0182] The mass fraction of insoluble matter in the sludge solidifying agent is ≤1.3%.

[0183] In the above-mentioned bottom mud purifier for in-situ degradation of river and lake bottom mud,

[0184] The preparation method of the sediment purifier is as follows:

[0185] (1) Wash the volcanic rock and PAC and use them as carriers;

[0186] (2) dispersing the carrier in a culture medium, adding the selected biological strain into the culture medium for expansion and cultivation, and centrifuging to obtain the carrier with in situ bacterial growth on its surface;

[0187] (3) Add the carrier of the in-situ growing bacteria on the surface to the sludge solidifying agent and continue stirring, and then let it stand for a period of time to obtain the product.

[0188] In the above-mentioned bottom mud purifier for in-situ degradation of river and lake bottom mud,

[0189] The culture medium described in step (2) is LB culture medium.

[0190] Comparative Example 1

[0191] The bottom mud purifier of this embodiment is used for in-situ degradation of river and lake bottom mud.

[0192] In parts by weight, it comprises the following components:

[0193]

[0194] Among them, the biological species include Pseudomonas aeruginosa;

[0195] The sludge solidifying agent includes polyacrylamide, anhydrous sodium sulfate, magnesium oxide and calcium oxide, and the mass ratio of the polyacrylamide, the anhydrous sodium sulfate, the magnesium oxide and the calcium oxide is 1:5:2:1.

[0196] In the above-mentioned bottom mud purifier for in-situ degradation of river and lake bottom mud,

[0197] The Pseudomonas aeruginosa was purchased from Shanghai Chuangjing Bio-Environmental Technology Co., Ltd.

[0198] In the above-mentioned bottom mud purifier for in-situ degradation of river and lake bottom mud,

[0199] The particle size of the volcanic rock is in the range of 3-6 mm;

[0200] The volcanic stone is black or red volcanic stone.

[0201] In the above-mentioned bottom mud purifier for in-situ degradation of river and lake bottom mud,

[0202] The PAC is industrial-grade black or yellow polyaluminium chloride, with a mass content of 28%.

[0203] In the above-mentioned bottom mud purifier for in-situ degradation of river and lake bottom mud,

[0204] The total colony count of the biological strain is ≥30 cfu / g.

[0205] In the above-mentioned bottom mud purifier for in-situ degradation of river and lake bottom mud,

[0206] The sludge solidifying agent is a gray powder;

[0207] The particle size range of the sludge solidifying agent is 100 mesh to 200 mesh;

[0208] The mass fraction of insoluble matter in the sludge solidifying agent is ≤1.3%.

[0209] In the above-mentioned bottom mud purifier for in-situ degradation of river and lake bottom mud,

[0210] The preparation method of the bottom mud purifier is as follows:

[0211] (1) Wash the volcanic rock and PAC and use them as carriers;

[0212] (2) dispersing the carrier in a culture medium, adding the selected biological strain into the culture medium for expansion and cultivation, and centrifuging to obtain the carrier with in situ bacterial growth on its surface;

[0213] (3) Add the carrier of the in-situ growing bacteria on the surface to the sludge solidifying agent and continue stirring, and then let it stand for a period of time to obtain the product.

[0214] In the above-mentioned bottom mud purifier for in-situ degradation of river and lake bottom mud,

[0215] The culture medium described in step (2) is LB culture medium.

[0216] Comparative Example 2

[0217] The bottom mud purifier of this embodiment is used for in-situ degradation of river and lake bottom mud.

[0218] In parts by weight, it comprises the following components:

[0219] 40 parts of volcanic stone,

[0220] PAC 30 copies,

[0221] 10 copies of biological strains;

[0222] The biological strains include Pseudomonas aeruginosa, Bacillus subtilis and Aspergillus niger, and the mass ratio of the Pseudomonas aeruginosa, the Bacillus subtilis and the Aspergillus niger is 1:2:5;

[0223] In the above-mentioned bottom mud purifier for in-situ degradation of river and lake bottom mud,

[0224] The Pseudomonas aeruginosa, the Bacillus subtilis and the Aspergillus niger were all purchased from Shanghai Chuangjing Bio-Environmental Protection Technology Co., Ltd.

[0225] In the above-mentioned bottom mud purifier for in-situ degradation of river and lake bottom mud,

[0226] The particle size of the volcanic rock is in the range of 3-6 mm;

[0227] The volcanic stone is black or red volcanic stone.

[0228] In the above-mentioned bottom mud purifier for in-situ degradation of river and lake bottom mud,

[0229] The PAC is industrial-grade black or yellow polyaluminium chloride, with a mass content of 28%.

[0230] In the above-mentioned bottom mud purifier for in-situ degradation of river and lake bottom mud,

[0231] The total colony count of the biological strain is ≥30 cfu / g.

[0232] In the above-mentioned bottom mud purifier for in-situ degradation of river and lake bottom mud,

[0233] The preparation method of the sediment purifier is as follows:

[0234] (1) Wash the volcanic rock and PAC and use them as carriers;

[0235] (2) dispersing the carrier in a culture medium, adding the selected biological strain into the culture medium for expansion and cultivation, and centrifuging to obtain the carrier with in situ bacterial growth on its surface;

[0236] (3) The carrier with the in-situ growing fungi on the surface is continuously stirred and then allowed to stand for a period of time to obtain the product.

[0237] In the above-mentioned bottom mud purifier for in-situ degradation of river and lake bottom mud,

[0238] The culture medium described in step (2) is LB culture medium.

[0239] Comparative Example 3

[0240] The bottom mud purifier of this embodiment is used for in-situ degradation of river and lake bottom mud.

[0241] In parts by weight, it comprises the following components:

[0242] 40 parts of volcanic stone,

[0243] PAC 30 copies,

[0244] 20 parts of sludge solidifying agent;

[0245] The sludge solidifying agent includes polyacrylamide, anhydrous sodium sulfate, magnesium oxide and calcium oxide, and the mass ratio of the polyacrylamide, the anhydrous sodium sulfate, the magnesium oxide and the calcium oxide is 1:5:2:1.

[0246] In the above-mentioned bottom mud purifier for in-situ degradation of river and lake bottom mud,

[0247] The Pseudomonas aeruginosa, the Bacillus subtilis and the Aspergillus niger were all purchased from Shanghai Chuangjing Bio-Environmental Protection Technology Co., Ltd.

[0248] In the above-mentioned bottom mud purifier for in-situ degradation of river and lake bottom mud,

[0249] The particle size of the volcanic rock is in the range of 3-6 mm;

[0250] The volcanic stone is black or red volcanic stone.

[0251] In the above-mentioned bottom mud purifier for in-situ degradation of river and lake bottom mud,

[0252] The PAC is industrial-grade black or yellow polyaluminium chloride, with a mass content of 28%.

[0253] In the above-mentioned bottom mud purifier for in-situ degradation of river and lake bottom mud,

[0254] The sludge solidifying agent is a gray powder;

[0255] The particle size range of the sludge solidifying agent is 100 mesh to 200 mesh;

[0256] The mass fraction of insoluble matter in the sludge solidifying agent is ≤1.3%.

[0257] In the above-mentioned bottom mud purifier for in-situ degradation of river and lake bottom mud,

[0258] The preparation method of the bottom mud purifier is as follows:

[0259] (1) Wash the volcanic rock and PAC and use them as carriers;

[0260] (2) Add the carrier to the sludge solidifying agent and continue stirring, then let it stand for a period of time to obtain the product.

[0261] Comparative Example 4

[0262] The bottom mud purifier of this embodiment is used for in-situ degradation of river and lake bottom mud.

[0263] In parts by weight, it comprises the following components:

[0264]

[0265] Among them, the biological strain is Bacillus subtilis;

[0266] The sludge solidifying agent includes polyacrylamide, anhydrous sodium sulfate, magnesium oxide and calcium oxide, and the mass ratio of the polyacrylamide, the anhydrous sodium sulfate, the magnesium oxide and the calcium oxide is 1:5:2:1.

[0267] In the above-mentioned bottom mud purifier for in-situ degradation of river and lake bottom mud,

[0268] The Bacillus subtilis was purchased from Shanghai Chuangjing Bio-Environmental Protection Technology Co., Ltd.

[0269] In the above-mentioned bottom mud purifier for in-situ degradation of river and lake bottom mud,

[0270] The particle size of the volcanic rock is in the range of 3-6 mm;

[0271] The volcanic stone is black or red volcanic stone.

[0272] In the above-mentioned bottom mud purifier for in-situ degradation of river and lake bottom mud,

[0273] The PAC is industrial-grade black or yellow polyaluminium chloride, with a mass content of 28%.

[0274] In the above-mentioned bottom mud purifier for in-situ degradation of river and lake bottom mud,

[0275] The total colony count of the biological strain is ≥30 cfu / g.

[0276] In the above-mentioned bottom mud purifier for in-situ degradation of river and lake bottom mud,

[0277] The sludge solidifying agent is a gray powder;

[0278] The particle size range of the sludge solidifying agent is 100 mesh to 200 mesh;

[0279] The mass fraction of insoluble matter in the sludge solidifying agent is ≤1.3%.

[0280] In the above-mentioned bottom mud purifier for in-situ degradation of river and lake bottom mud,

[0281] The preparation method of the bottom mud purifier is as follows:

[0282] (1) Wash the volcanic rock and PAC and use them as carriers;

[0283] (2) dispersing the carrier in a culture medium, adding the selected biological strain into the culture medium for expansion and cultivation, and centrifuging to obtain the carrier with in situ bacterial growth on its surface;

[0284] (3) Add the carrier of the in-situ growing bacteria on the surface to the sludge solidifying agent and continue stirring, and then let it stand for a period of time to obtain the product.

[0285] In the above-mentioned bottom mud purifier for in-situ degradation of river and lake bottom mud,

[0286] The culture medium described in step (2) is LB culture medium.

[0287] Comparative Example 5

[0288] The bottom mud purifier of this embodiment is used for in-situ degradation of river and lake bottom mud.

[0289] In parts by weight, it comprises the following components:

[0290]

[0291] Among them, the biological species is Aspergillus niger;

[0292] The sludge solidifying agent includes polyacrylamide, anhydrous sodium sulfate, magnesium oxide and calcium oxide, and the mass ratio of the polyacrylamide, the anhydrous sodium sulfate, the magnesium oxide and the calcium oxide is 1:5:2:1.

[0293] In the above-mentioned bottom mud purifier for in-situ degradation of river and lake bottom mud,

[0294] The Aspergillus niger was purchased from Shanghai Chuangjing Bio-Environmental Protection Technology Co., Ltd.

[0295] In the above-mentioned bottom mud purifier for in-situ degradation of river and lake bottom mud,

[0296] The particle size of the volcanic rock is in the range of 3-6 mm;

[0297] The volcanic stone is black or red volcanic stone.

[0298] In the above-mentioned bottom mud purifier for in-situ degradation of river and lake bottom mud,

[0299] The PAC is industrial-grade black or yellow polyaluminium chloride, with a mass content of 28%.

[0300] In the above-mentioned bottom mud purifier for in-situ degradation of river and lake bottom mud,

[0301] The total colony count of the biological strain is ≥30 cfu / g.

[0302] In the above-mentioned bottom mud purifier for in-situ degradation of river and lake bottom mud,

[0303] The sludge solidifying agent is a gray powder;

[0304] The particle size range of the sludge solidifying agent is 100 mesh to 200 mesh;

[0305] The mass fraction of insoluble matter in the sludge solidifying agent is ≤1.3%.

[0306] In the above-mentioned bottom mud purifier for in-situ degradation of river and lake bottom mud,

[0307] The preparation method of the sediment purifier is as follows:

[0308] (1) Wash the volcanic rock and PAC and use them as carriers;

[0309] (2) dispersing the carrier in a culture medium, adding the selected biological strain into the culture medium for expansion and cultivation, and centrifuging to obtain the carrier with in situ bacterial growth on its surface;

[0310] (3) Add the carrier of the in-situ growing bacteria on the surface to the sludge solidifying agent and continue stirring, and then let it stand for a period of time to obtain the product.

[0311] In the above-mentioned bottom mud purifier for in-situ degradation of river and lake bottom mud,

[0312] The culture medium described in step (2) is LB culture medium.

[0313] Example 6

[0314] The method of using the sediment purifier is as follows:

[0315] Calculation of the amount of sediment purifier

[0316] Dosage of sludge purifier: 450g / m 2 (River area)

[0317] Generally, it is added in three times within three months. In the first month, the first addition is 250g / m 2 In the remaining two months, 100g / m 2 .

[0318] Method of adding sediment purifier-

[0319] The method of adding sediment purifier is:

[0320] (1) Clearly define the release area: Clearly define the release area based on the actual pollution situation of the river;

[0321] (2) Determine the dosing time: It is required to choose the non-rainy season to ensure that the water flow rate is low and the sediment content is low;

[0322] (3) Dosing of the drug: Use simple manual dosing equipment to add the drug in batches in the appropriate area, and pay attention to even spreading when adding.

[0323] The application location of sediment purifier-

[0324] A black and smelly river in the urban area of ​​Guangzhou.

[0325] Effect of applying sediment purifier-

[0326] Example 1: COD removal rate is 92%;

[0327] Example 2: COD removal rate is 93%;

[0328] Example 3: COD removal rate is 92%;

[0329] Example 4: COD removal rate is 93%;

[0330] Example 5: COD removal rate is 94%;

[0331] Comparative Example 1: COD removal rate was 59%;

[0332] Comparative Example 2: COD removal rate was 45%;

[0333] Comparative Example 3: COD removal rate was 31%;

[0334] Comparative Example 4: COD removal rate was 48%;

[0335] Comparative Example 5: COD removal rate was 53%.

[0336] It should be noted that Comparative Example 1 retains Pseudomonas aeruginosa, Comparative Example 4 retains Bacillus subtilis, and Comparative Example 5 retains Aspergillus niger. It can be seen that by retaining a single strain, the COD removal rate drops significantly, which may be related to the synergistic performance of the composite strain. We subsequently conducted tests, retaining the test group of Pseudomonas aeruginosa and Bacillus subtilis, retaining the test group of Pseudomonas aeruginosa and Aspergillus niger, and retaining the test group of Bacillus subtilis and Aspergillus niger. After analysis, their COD removal rates are in the range of 66%-73. It can be seen that the strains of this application are not selected at random, and need to be used in combination with volcanic rock, PAC and sludge solidifier in the bottom mud purifier to play an important role. Selecting inappropriate strains may bring about antagonism between strains, which will bring adverse effects.

[0337] At the same time, Comparative Example 2 removed the sludge solidifying agent, and Comparative Example 3 did not add any degradation bacteria at all. It can be found that the COD removal rates of the two comparative examples are in a lower range. This shows that, on the one hand, the sludge solidifying agent may play a role in the overall stability of the bottom mud purifier, which can prevent the bottom mud purifier from being washed away by the water flow at the bottom of the water, so that the bottom mud purifier can completely purify the water body. On the other hand, after the biological bacteria are completely removed, the COD removal rate reaches the lowest. It can be seen that it is difficult to effectively purify by relying solely on components such as volcanic rocks and PAC.

[0338] In addition, the sediment purifier prepared in Example 5 was tested in practice. Figure 1 and Figure 2 As shown, before the treatment, the river was very black and smelly, with a silt thickness of about 70 cm. After the treatment, the river water became clear, and fish and other animals lived there.

[0339] At the same time, combined Figure 3 As can be seen from Figures A and B, the sediment purifier is a solid powder. Through our research and development, we have developed two new products, as shown in Table 1.

[0340] Table 1

[0341] model state density Remark XS / SPP-A Solid State <![CDATA[1.1kg / m 3 ]]> Basic type XS / SPP-B Solid State <![CDATA[1.2kg / m 3 ]]> Enhanced

[0342] This sediment purifier is a composite of selected minerals and immobilized sediment-degrading bacteria. It appears as gray sand and comes in two types: A and B. Type A is primarily used for decomposing and oxidizing sediment in general water bodies, while Type B is designed for decomposing and oxidizing sediment in heavily contaminated water bodies and thicker sludge.

[0343] For example Figure 4 and Figure 5 As shown, the black and smelly river uses the bottom mud purifier of the present application, and the river changes from before treatment (severe black and smelly) to after treatment (the mud surface turns grayish white).

[0344] After laboratory analysis, the parameters of the prepared sediment purifier are shown in Table 2. It should be noted that: (1) This product is a biological bacteria compound preparation, does not contain any toxic or harmful chemicals, and will not cause secondary pollution to the environment; (2) The solidified microorganisms are beneficial bacteria such as organic matter degrading bacteria and nitrifying bacteria.

[0345] Table 2

[0346]

[0347]

[0348] In summary, combined Figure 6 From the perspective of the preparation, the sediment purifier has the following characteristics:

[0349] (1) The interior of the framework is filled with micropores and channels (more than 1 million per cubic micron), which can provide a huge surface area for the attachment of microbial communities in the water.

[0350] (2) The internal pore structure also has the function of screening molecules, which can select dominant microbial populations and help microorganisms reproduce rapidly.

[0351] (3) It has a sedimentation function and sinks quickly to the bottom of the water. It and the microorganisms attached to it are not easily lost.

[0352] (4) It works quickly, quickly decomposing and strengthening the silt in the water, achieving the effect of eliminating odors.

[0353] (5) It is easy to use, has no secondary pollution, and is compatible with other water treatment technologies, greatly reducing the difficulty of treatment and improving the treatment effect.

[0354] It will be apparent to those skilled in the art that the present invention is not limited to the details of the exemplary embodiments described above and that the invention can be embodied in other specific forms without departing from the spirit or essential characteristics of the invention. Therefore, the embodiments should be considered in all respects as illustrative and non-restrictive, and the scope of the invention is defined by the appended claims rather than the foregoing description, and it is intended that all variations that come within the meaning and range of equivalents of the claims be embraced therein.

[0355] In addition, it should be understood that although this specification is described in terms of implementation methods, not every implementation method contains only one independent technical solution. This narrative method of the specification is only for the sake of clarity. Those skilled in the art should regard the specification as a whole. The technical solutions in each embodiment can also be appropriately combined to form other implementation methods that can be understood by those skilled in the art.

Claims

1. A sediment purifier for in-situ degradation of river and lake bottom silt, characterized by: In parts by weight, it comprises the following components: 35-45 parts of volcanic rock, PAC 25-35 copies, 5-15 parts of biological strains, 15-25 parts of sludge solidifying agent; The biological strains include Pseudomonas aeruginosa, Bacillus subtilis and Aspergillus niger, and the mass ratio of the Pseudomonas aeruginosa, the Bacillus subtilis and the Aspergillus niger is 1:2:5; The sludge solidifying agent includes polyacrylamide, anhydrous sodium sulfate, magnesium oxide and calcium oxide, and the mass ratio of the polyacrylamide, the anhydrous sodium sulfate, the magnesium oxide and the calcium oxide is 1:5:2:

1.

2. The sediment purifier for in-situ degradation of river and lake bottom sludge according to claim 1, characterized in that: In parts by weight, it comprises the following components: 38-42 parts of volcanic rock, PAC 28-32 copies, 8-12 parts of biological strains, 18-22 parts of sludge solidifying agent; The biological strains include Pseudomonas aeruginosa, Bacillus subtilis and Aspergillus niger, and the mass ratio of the Pseudomonas aeruginosa, the Bacillus subtilis and the Aspergillus niger is 1:2:5; The sludge solidifying agent includes polyacrylamide, anhydrous sodium sulfate, magnesium oxide and calcium oxide, and the mass ratio of the polyacrylamide, the anhydrous sodium sulfate, the magnesium oxide and the calcium oxide is 1:5:2:

1.

3. The sediment purifier for in-situ degradation of river and lake bottom sludge according to claim 2, characterized in that: In parts by weight, it comprises the following components: 40 parts of volcanic stone, PAC 30 copies, 10 copies of biological strains, 20 parts of sludge solidifying agent; The biological strains include Pseudomonas aeruginosa, Bacillus subtilis and Aspergillus niger, and the mass ratio of the Pseudomonas aeruginosa, the Bacillus subtilis and the Aspergillus niger is 1:2:5; The sludge solidifying agent includes polyacrylamide, anhydrous sodium sulfate, magnesium oxide and calcium oxide, and the mass ratio of the polyacrylamide, the anhydrous sodium sulfate, the magnesium oxide and the calcium oxide is 1:5:2:

1.

4. The sediment purifier for in-situ degradation of river and lake bottom sludge according to claim 1, characterized in that: The particle size of the volcanic rock is in the range of 3-6 mm; The volcanic stone is black or red volcanic stone.

5. The sediment purifier for in-situ degradation of river and lake bottom sludge according to claim 1, characterized in that: The PAC is industrial-grade yellow polyaluminium chloride with a mass content of 28%.

6. The sediment purifier for in-situ degradation of river and lake bottom sludge according to claim 1, characterized in that: The total colony count of the biological strain is ≥30 cfu / g.

7. The sediment purifier for in-situ degradation of river and lake bottom sludge according to claim 1, characterized in that: The sludge solidifying agent is a gray powder; The particle size range of the sludge solidifying agent is 100 mesh to 200 mesh; The mass fraction of insoluble matter in the sludge solidifying agent is ≤1.3%.

8. The sediment purifier for in-situ degradation of river and lake bottom sludge according to claim 1, characterized in that: The preparation method of the bottom mud purifier is as follows: (1) Wash the volcanic rock and PAC and use them as carriers; (2) Dispersing the carrier in a culture medium, adding the selected biological strain into the culture medium for expansion and cultivation, and centrifuging to obtain the carrier with in situ growth of bacteria on the surface; (3) Add the carrier of the surface in situ growing bacteria to the sludge solidifying agent and stir it continuously, then let it stand for a period of time to obtain the product.

9. The sediment purifier for in-situ degradation of river and lake bottom sludge according to claim 8, characterized in that: The culture medium described in step (2) is LB culture medium.

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