Ganoderma lucidum strain highly resistant to cobweb disease throughout the growth period

Abstract

The present invention relates to a Ganoderma lucidum strain with high resistance to cobweb disease throughout the whole growth period. The preservation number of the Ganoderma lucidum strain with high resistance to cobweb disease throughout the whole growth period is CCTCC No: M 20221337; this Ganoderma lucidum strain has high resistance to cobweb disease throughout the whole growth period, can effectively reduce the degree and epidemic speed of cobweb disease, is suitable for large-scale cultivation, and can generate excellent economic and social benefits.

Description

Technical Field

[0001] The present invention relates to the field of microorganisms, and particularly to Ganoderma lucidum strains with high resistance to cobweb disease throughout the whole growth period. Background Art

[0002] Ganoderma lucidum, also known as Ganoderma rubra and Danzhi, is a traditional medicinal fungus with the effects of nourishing and strengthening the body and consolidating the healthy qi. It has been used as medicine for more than two thousand years. The scale of artificial cultivation of Ganoderma lucidum has been expanding year by year. However, with the booming development of the Ganoderma lucidum industry, the problem of diseases has become increasingly prominent. Among them, cobweb disease is the main disease restricting the development of the Ganoderma lucidum industry, and its pathogen is Cladobotryum mycophilum. The incidence of cobweb disease extends from the initial stage to the final stage of spore production in Ganoderma lucidum, and with the increase in rainfall, the spread accelerates, causing serious economic losses. In severely affected areas, the incidence of cobweb disease is as high as 33.3%, resulting in serious losses of Ganoderma lucidum spore powder raw powder.

[0003] Selecting new Ganoderma lucidum varieties with high resistance is the most direct and effective means to achieve disease control. However, most of the current cultivated strains are highly susceptible strains. Therefore, carrying out research on disease resistance and selecting new disease-resistant germplasms are key measures to reduce the degree and epidemic speed of diseases, reduce economic losses, and ensure the development and growth of the Ganoderma lucidum industry. Summary of the Invention

[0004] Based on this, it is necessary to provide a Ganoderma lucidum strain with high resistance to cobweb disease throughout the whole growth period in view of the above problems; this Ganoderma lucidum strain has high resistance to cobweb disease throughout the whole growth period, can effectively reduce the degree and epidemic speed of cobweb disease, is suitable for large-scale cultivation, and can produce excellent economic and social benefits.

[0005] The present invention provides a Ganoderma lucidum strain with high resistance to cobweb disease throughout the whole growth period, and its deposit number is CCTCC No: M20221337.

[0006] A breeding method for a Ganoderma lucidum strain with high resistance to cobweb disease throughout the whole growth period as described above includes the following steps:

[0007] Providing a monokaryon of "X2-18" and a monokaryon of "Japanese red Ganoderma lucidum"; and

[0008] Performing a single-to-single hybridization between the monokaryon of "X2-18" and the monokaryon of "Japanese red Ganoderma lucidum", and screening out the hybrid with clamp connections to obtain a Ganoderma lucidum strain with high resistance to cobweb disease throughout the whole growth period.

[0009] In one embodiment, the step of providing a monokaryon of "X2-18" and a monokaryon of "Japanese red Ganoderma lucidum" includes:

[0010] Providing a protoplast suspension of "X2-18" and a protoplast suspension of "Japanese red Ganoderma lucidum";

[0011] The protoplast suspensions of the "X2-18" and the "Ganoderma lucidum (Curtis) P. Karst. var. rubropileatum Imaz." are respectively subjected to regeneration culture to obtain the regenerated colonies of the "X2-18" and the regenerated colonies of the "Ganoderma lucidum (Curtis) P. Karst. var. rubropileatum Imaz."; and

[0012] Colonies without clamp connections are screened out to obtain the monokaryons of the "X2-18" and the monokaryons of the "Ganoderma lucidum (Curtis) P. Karst. var. rubropileatum Imaz.".

[0013] Use of a Ganoderma lucidum strain with high resistance to cobweb disease throughout the growth period as described above in breeding.

[0014] A Ganoderma lucidum mycelium, which is obtained by culturing the Ganoderma lucidum strain with high resistance to cobweb disease throughout the growth period as described above.

[0015] In one embodiment, the Ganoderma lucidum mycelium has an antagonistic effect on the mycelium of Botrytis myophilum and inhibits the mycelial growth of Botrytis myophilum.

[0016] A Ganoderma lucidum fruit body, which is obtained by cultivating the Ganoderma lucidum mycelium as described above.

[0017] In one embodiment, the incidence rate of cobweb disease of the Ganoderma lucidum fruit body is less than or equal to 8.33%.

[0018] A cultivation method of the Ganoderma lucidum fruit body as described above, comprising the following steps:

[0019] Provide a liquid spawn and / or a solid spawn including the Ganoderma lucidum mycelium as described above;

[0020] Inoculate the liquid spawn and / or the solid spawn into a spawn bag for cultivation;

[0021] After the mycelium fills the bag, continue to place the spawn bag with the mycelium for after-ripening; and

[0022] Transfer the after-ripened spawn bag to light for fruiting cultivation to obtain the Ganoderma lucidum fruit body.

[0023] In one embodiment, in the step of fruiting cultivation, the relative humidity is 75%-90%, and the daily light time is 8h-14h.

[0024] The Ganoderma lucidum strain with high resistance to cobweb disease throughout the growth period provided by the present invention is such that the growth of the Ganoderma lucidum mycelium obtained by culturing it is not affected by Botrytis myophilum, and the Ganoderma lucidum fruit body is not easily infected by Botrytis myophilum. It can be seen that the Ganoderma lucidum strain provided by the present invention has high resistance to cobweb disease throughout the growth period, can effectively reduce the degree and epidemic speed of cobweb disease, is suitable for large-scale cultivation, and can produce excellent economic and social benefits. Description of the Drawings

[0025] Figure 1 The confrontation culture in petri dishes of the Ganoderma lucidum strain GZ206 provided by the present invention and "Ganoderma lucidum 78" with Cladosporium cladosporioides. A and B represent the test groups of the Ganoderma lucidum strain GZ206 provided by the present invention, and C and D represent the control groups of "Ganoderma lucidum 78". g represents Ganoderma lucidum, and c represents Cladosporium cladosporioides;

[0026] Figure 2 The disease incidence of the Ganoderma lucidum strain GZ206 provided by the present invention and "Ganoderma lucidum 78" after inoculating Cladosporium cladosporioides during the flake-opening period. A represents the test group of the Ganoderma lucidum strain GZ206 provided by the present invention, and B represents the control group of "Ganoderma lucidum 78". Detailed implementation manners

[0027] To facilitate the understanding of the present invention, the present invention will be described more comprehensively below with reference to relevant embodiments. However, the present invention can be implemented in many different forms and is not limited to the embodiments described herein. On the contrary, these embodiments are provided to make the understanding of the disclosure of the present invention more thorough and comprehensive.

[0028] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by those skilled in the technical field to which the present invention belongs. The terms used in the description of the present invention herein are only for the purpose of describing specific embodiments and are not intended to limit the present invention. The term "and / or" used herein includes any and all combinations of one or more of the related listed items.

[0029] The Ganoderma lucidum strain with high resistance to cobweb disease throughout the growth period provided by the present invention will be further described below.

[0030] The Ganoderma lucidum strain with high resistance to cobweb disease throughout the growth period provided by the present invention:

[0031] Name: Ganoderma lucidum strain GZ206;

[0032] Taxonomic naming: Ganoderma lucidum;

[0033] Latin scientific name: Ganoderma lucidum sensu auct.sina,non G.lucidum(Curtis)P.Karst.1881(correct name: G.sichuanense J.D.Zhao&X.Q.Zhang 1983;synonym: G.lingzhi Sheng H.Wu,Y.Cao&Y.C.Dai 2012).

[0034] It was deposited at the China Center for Type Culture Collection (CCTCC) on August 26, 2022. The address is: No. 299, Bayi Road, Wuchang District, Wuhan City, Hubei Province; the deposit number is CCTCC No: M 20221337.

[0035] The method for breeding Ganoderma lucidum strains provided by the present invention includes the following steps:

[0036] S10, providing monokaryons of "X2-18" and monokaryons of "Japanese Red Ganoderma"; and

[0037] S20, performing single-to-single hybridization between the monokaryons of "X2-18" and the monokaryons of "Japanese Red Ganoderma", and screening out the hyphae with clamp connections to obtain Ganoderma lucidum strains.

[0038] In step S10, "X2-18" was deposited at the China Center for Type Culture Collection (CCTCC) on November 10, 2022, classified as Ganoderma lucidum. The address is: No. 299, Bayi Road, Wuchang District, Wuhan City, Hubei Province; the deposit number is CCTCC No: M 20221751; "Japanese Red Ganoderma" can be purchased.

[0039] In one embodiment, step S10 specifically includes the following steps:

[0040] S101, providing a protoplast suspension of "X2-18" and a protoplast suspension of "Japanese Red Ganoderma";

[0041] S102, respectively performing regeneration culture on the protoplast suspension of "X2-18" and the protoplast suspension of "Japanese Red Ganoderma" to obtain the regenerated colonies of "X2-18" and the regenerated colonies of "Japanese Red Ganoderma"; and

[0042] S103, screening out the colonies without clamp connections to obtain the monokaryons of "X2-18" and the monokaryons of "Japanese Red Ganoderma".

[0043] In step S20, it should be noted that clamp connection is the identification standard for Ganoderma lucidum dikaryotic mycelium. Any mycelium that forms a clamp connection can be determined as a dikaryon.

[0044] The present invention uses the protoplast monokaryotization technology to obtain the monokaryons of Ganoderma lucidum strain "X2-18" and the monokaryons of "Japanese Red Ganoderma", and performs single-to-single hybridization using the two monokaryons to obtain Ganoderma lucidum strain GZ206, which is further used for cultivating Ganoderma lucidum fruit bodies.

[0045] The present invention provides the application of Ganoderma lucidum strain GZ206 in breeding.

[0046] When the Ganoderma lucidum strain GZ206 is applied to breeding, the Ganoderma lucidum strain GZ206 is used as a parent for breeding.

[0047] The present invention also provides a Ganoderma lucidum mycelium, which is obtained by culturing the Ganoderma lucidum strain GZ206 as described above; it can be understood that the mycelium is composed of many hyphae aggregated together to form the vegetative body of the fungus.

[0048] In one embodiment, the steps of culturing the Ganoderma lucidum strain GZ206 to obtain the Ganoderma lucidum mycelium include: activating the preserved GZ206 test-tube strain, and transferring and expanding the culture after activation to obtain the GZ206 mycelium.

[0049] The growth of the Ganoderma lucidum mycelium provided by the present invention is not affected by Botrytis cinerea. When the Ganoderma lucidum mycelium comes into contact with Botrytis cinerea, the growth rate of the Ganoderma lucidum mycelium is not inhibited, no pigment is produced, and the colony does not degrade; after the Ganoderma lucidum mycelium comes into contact with Botrytis cinerea, the growth rate of the mycelium of the Ganoderma lucidum strain GZ206 is still not inhibited; in one embodiment, the Ganoderma lucidum mycelium stage has an antagonistic effect on the mycelium of the pathogen of cobweb disease (Botrytis cinerea), inhibiting the mycelial growth of Botrytis cinerea.

[0050] The present invention also provides a Ganoderma lucidum fruit body, which is obtained by cultivating the Ganoderma lucidum mycelium as described above; it can be understood that the fruit body is the spore-bearing structure of higher fungi and is composed of organized mycelium.

[0051] The Ganoderma lucidum fruit body provided by the present invention is not easily infected by Botrytis cinerea. In one embodiment, the incidence rate of the Ganoderma lucidum fruit body provided by the present invention is as low as below 8.33%.

[0052] The present invention also provides a cultivation method of the Ganoderma lucidum fruit body as described above, including the following steps:

[0053] S10, providing a liquid spawn and / or a solid spawn including Ganoderma lucidum mycelium;

[0054] S20, inoculating the liquid spawn and / or the solid spawn into a mushroom bag for cultivation;

[0055] S30, after the mycelium fills the bag, continuing to place the mushroom bag with mycelium for afterripening; and

[0056] S40, transferring the afterripened mushroom bag to the light for fruiting cultivation to obtain the Ganoderma lucidum fruit body.

[0057] In step S10, compared with the liquid spawn, the solid spawn has the advantages of a short production cycle, convenient use, and simple operation. The steps of providing the solid spawn specifically include: inoculating the Ganoderma lucidum mycelium into the culture material and obtaining the solid spawn after culturing for 12d - 14d.

[0058] In one embodiment, the culture medium includes oak sawdust, wheat bran, lime, gypsum and water; preferably, the mass fraction of oak sawdust in the culture medium is 47%-51%, the mass fraction of wheat bran in the culture medium is 10%-14%, the mass fraction of lime in the culture medium is 0.4%-0.8%, the mass fraction of gypsum in the culture medium is 0.4%-0.8%, and the mass fraction of water in the culture medium is 34%-38%.

[0059] To avoid the influence of other microorganisms on the growth of Ganoderma lucidum mycelium, the culture medium is first sterilized by a high-pressure steam sterilizer, and then the sterilized culture medium is placed in a sterile environment and cooled to room temperature; in one embodiment, in the step of sterilizing with a high-pressure steam sterilizer, the temperature is 115°C - 127°C and the time is 110 min - 130 min.

[0060] In step S20, to better cultivate Ganoderma lucidum mycelium, in one embodiment, the mushroom bag includes oak sawdust, wheat bran, lime and gypsum; preferably, the mass fraction of oak sawdust in the mushroom bag is 73%-83%, the mass fraction of wheat bran in the mushroom bag is 15%-25%, the mass fraction of lime in the mushroom bag is 0.5%-1.5%, and the mass fraction of gypsum in the mushroom bag is 0.5%-1.5%.

[0061] In one embodiment, before the step of inoculating the liquid strain or solid strain into the mushroom bag for cultivation, water is added to the mushroom bag, and the mass ratio of the mushroom bag to water is 1:0.55 - 1:0.6.

[0062] To avoid the influence of other microorganisms on the growth of Ganoderma lucidum mycelium, the mushroom bag after adding water is first sterilized by a high-pressure steam sterilizer, and then placed in a sterile environment and cooled to room temperature; in one embodiment, in the step of sterilizing with a high-pressure steam sterilizer, the temperature is 115°C - 127°C and the time is 110 min - 130 min.

[0063] To provide sufficient nutrients for Ganoderma lucidum mycelium, in one embodiment, the mass ratio of the mushroom bag to the solid strain is 795:15 - 805:15.

[0064] In one embodiment, in the step of cultivation, the temperature is 25°C - 28°C, the relative humidity is 50%-60%, and the CO2 concentration is not higher than 400 ppm.

[0065] It can be understood that during the cultivation process, the mushroom bag is placed in the dark condition.

[0066] In step S30, in one embodiment, it takes 12 d - 16 d to achieve full colonization of the mycelium.

[0067] In step S40, during the step of fruiting cultivation, the relative humidity is 75%-90%, and the daily lighting time is 8h-14h.

[0068] The Ganoderma lucidum strain provided by the present invention has high resistance to cobweb disease throughout the whole growth period, can effectively reduce the occurrence degree and epidemic speed of cobweb disease, is suitable for large-scale cultivation, and can produce excellent economic and social benefits.

[0069] The present invention will be further described below in conjunction with specific embodiments. It should be understood that the specific embodiments described herein are only used to explain the embodiments of the present invention and are not used to limit the embodiments of the present invention.

[0070] Example 1

[0071] Preparation of protoplast suspension: Inoculate the dikaryotic strains "X2-18" and "Ganoderma rubra from Japan" into the sterilized PDA liquid medium respectively, and culture them under dark conditions with shaking at 25°C and 150 rpm / min for 10d-15d; after the mycelial balls are cultured well, rinse the medium on the mycelial balls with ddH2O, rinse with the osmotic stabilizer solution, then place the mycelia on the sterilized filter paper to absorb the excess water, and collect the mycelia; add 2% lysing enzyme at 3 times the amount of the mycelia, and enzymolyze at 25°C for 4h to obtain a crude extract; centrifuge the filtrate obtained after filtration at 3000 rpm / min for 5 min, discard the supernatant, and the protoplast precipitate can be seen. Then wash it 2 times with the osmotic stabilizer solution, and suspend the precipitate with the osmotic stabilizer solution to prepare the protoplast suspensions of "X2-18" and "Ganoderma rubra from Japan" respectively.

[0072] Protoplast regeneration: Take the protoplast suspensions of "X2-18" and "Ganoderma rubra from Japan", spread them evenly on the regeneration medium respectively, and culture them under dark conditions at 25°C for 168h-240h, observe their regeneration situation. When tiny colonies appear on the regeneration medium, transfer the colonies to the PDA medium petri dish.

[0073] Monokaryon screening: After the picked colonies grow slightly, examine them under a microscope to observe whether there are clamp connections. If there are no clamp connections, use DAPI dye to perform fluorescence staining identification on their nuclear phases, and finally determine the monokaryotic strains to obtain the monokaryon "X2-18-01" of "X2-18" and the monokaryon "Ganoderma rubra from Japan-01" of Ganoderma rubra from Japan.

[0074] Single-single hybridization: Hybridize "X2-18-01" and "Ganoderma rubra from Japan-01" pairwise, pick the mycelia on the outer side of the two strain blocks for microscopic examination, and judge whether the hybridization is successful by the presence or absence of clamp connections. Screen the mycelia with clamp connections to obtain the Ganoderma lucidum strain GZ206.

[0075] The genomic DNA of Ganoderma lucidum strain GZ206 was extracted using a novel genomic DNA extraction kit, and PCR amplification was performed using the fungal universal primers ITS1 / ITS4.

[0076] Primer sequences:

[0077] ITS1 5'-TCCGTAGGTGAACCTGCGG-3'

[0078] ITS4 5'-TCCTCCGCTTATTGATATGC-3'

[0079] PCR amplification reaction system and conditions:

[0080] The PCR reaction system was 25 μL, including 2.5 μL of 10×PCR Buffer, 2 μL of 25 mmol / L MgCl2, 0.6 μL of 2.5 mmol / L dNTP, 1 μL each of primers ITS1 / ITS4, 1 μL of DNA template, 0.3 μL of Taq enzyme, and sterile distilled water was added to make up the volume.

[0081] The PCR reaction conditions were: pre-denaturation at 94°C for 5 min; denaturation at 94°C for 50 s, annealing at 56°C for 50 s, extension at 72°C for 1 min, for 28 cycles; extension at 72°C for 10 min.

[0082] The samples that passed the agarose gel detection were sent to Sangon Biotech (Shanghai) Co., Ltd. for sequencing, and the sequencing results of Ganoderma lucidum strain GZ206 were compared on NCBI to confirm that Ganoderma lucidum strain GZ206 belongs to Ganoderma lucidum (Ganodermalucidum sensu auct.sina; = G.sihuanense; syn.: G.lingzhi).

[0083] Example 2

[0084] Strain preparation: To unify the inoculation amount of the fungus bags, solid strains with a short production cycle, convenient use, and simple operation were selected in this cultivation. The formula of the culture medium was 49.52% coarse oak sawdust, 12.7% wheat bran, 0.64% lime, 0.64% gypsum, and 36.5% water. Using tissue culture flasks (specification 80 mm × 122 mm), the mixed culture medium was added and sterilized at 121°C for 120 min using a high-pressure steam sterilizer, and then the sterilized tissue culture flasks were pushed into the cooling room in a sterile environment to cool to room temperature. Each tissue culture flask was inoculated with 1 / 8 petri dish (90 mm) of Ganoderma lucidum mycelium, and it could be fully grown after about 14 days of culturing the bacteria.

[0085] Spawn bag production: Soak the coarse oak sawdust 24 hours in advance. After sufficient soaking, mix in wheat bran, lime, and gypsum. The mass ratio of coarse oak sawdust, wheat bran, lime, and gypsum is 78%:20%:1%:1%. After thorough mixing, adjust the water content to between 55% - 60% and then bag it. Use 15 cm × 30 cm polypropylene spawn bags, with each bag filled with 0.8 ± 0.005 kg of material. Sterilize at 121 °C for 120 minutes using a high-pressure steam sterilizer. After sterilization, push the bags into the cooling room. Wait until the surface temperature of the spawn bag is lower than 20 °C before inoculation, and inoculate 15 g of solid spawn per bag.

[0086] Spawn cultivation and after-ripening: Vertically place the inoculated spawn bags on the spawn cultivation rack, control the temperature at 25 °C - 28 °C, relative humidity at 50% - 60%, CO2 concentration not higher than 400 ppm, and cultivate in the dark until the spawn bags are fully colonized. After the bags are fully colonized, continue to place them for one week for after-ripening.

[0087] Mushroom fruiting management: Enter the stage of inducing primordium formation, adjust the humidity in the mushroom house to a relative humidity of 75% - 90%, and at the same time give 12 hours of light per day to stimulate primordium differentiation and the formation of Ganoderma lucidum fruit bodies.

[0088] Test Example 1

[0089] Using "Ganoderma lucidum 78" (mutated from the Ganoderma lucidum 78 maternal strain collected from the wild) as a control, conduct Petri dish confrontation culture tests on Ganoderma lucidum strains GZ206, "Ganoderma lucidum 78", and Botrytis mycodophila respectively.

[0090] First, pick a 5-mm diameter mycelial block of Ganoderma lucidum strain GZ206 onto a 90-mm diameter Petri dish (the inoculation point is 30 mm away from the edge of the Petri dish) and culture at 25 °C. When the mycelial block of Ganoderma lucidum strain GZ206 grows to a colony diameter of about 30 mm, then pick a 5-mm diameter mycelial block of the pathogen (Botrytis mycodophila) onto the same Petri dish (the distance between the two mycelial blocks is about 30 mm); continue to culture at 25 °C.

[0091] Each confrontation test between Ganoderma lucidum strain GZ206 and Botrytis mycodophila is regarded as one treatment, with 3 parallel tests set for each treatment and repeated 3 times. The Petri dish confrontation culture situations of Ganoderma lucidum strain GZ206 (a Ganoderma lucidum strain highly resistant to cobweb disease throughout its growth period) and "Ganoderma lucidum 78" with Botrytis mycodophila are as Figure 1 shown.

[0092] During the mycelial confrontation stage, when the mycelium of Ganoderma lucidum strain GZ206 first came into contact with Botrytis mycodophila, the growth rate of the Ganoderma lucidum mycelium was not inhibited, no pigment was produced, the colony was not degraded, and no antagonistic line was seen. After contact, the growth rate of the mycelium of Ganoderma lucidum strain GZ206 was not inhibited, and an obvious antagonistic line was produced at the junction ( Figure 1A, B); The mycelial growth rate of "Ganoderma lucidum 78" was inhibited, and the mycelium of Botrytis ramicola covered the Ganoderma colony and continued to grow, with pigment production ( Figure 1 C, D).

[0093] Test Example 2

[0094] Take the Ganoderma lucidum fruiting bodies that have grown to the slicing stage, adopt the quantitative spray inoculation method. Take a 1 mL handheld spray bottle, disinfect it with 75% alcohol for 5 minutes, then rinse it with sterile water 3 times. Fill the prepared Botrytis ramicola spore suspension into the spray bottle. Place the Ganoderma lucidum bag with the fruiting body with the lower surface of the cap facing up on the vertical fruiting rack, and quantitatively and evenly spray 1 mL of the prepared pathogen spore suspension with a concentration of 1×10 5 CFU / mL on the lower surface of the cap of each tested Ganoderma lucidum fruiting body. After spraying the disease, place the lower surface of the cap downward 6 hours later. Each treatment has 3 replicates. Observe and record the disease occurrence of the Ganoderma lucidum fruiting bodies every 24 hours after inoculating Botrytis ramicola until the 7th day of monitoring is stopped.

[0095] Use the FScan2000 phenotype scanner to record the phenotypes of the inoculated Ganoderma lucidum fruiting bodies. The specific records include: morphological changes of the fruiting bodies, whether they are deformed, color changes, presence or absence of pathogen colonies and spore formation, and lesion area. Calculate the incidence and disease index of the Ganoderma lucidum strain GZ206 (a Ganoderma lucidum strain with high resistance to cobweb disease during the whole growth period) and "Ganoderma lucidum 78" respectively according to the formulas, and judge their disease resistance and susceptibility. The specific results are shown in Table 1.

[0096] The formula for calculating the incidence (F, %) is:

[0097]

[0098] In the formula: G is the number of diseased fruiting bodies (pieces); Z is the total number of fruiting bodies (pieces).

[0099] The formula for calculating the disease index (D, %) is:

[0100]

[0101] In the formula: J is the level of the disease susceptibility grade of the Ganoderma lucidum investigated in the plot; G is the number of Ganoderma lucidum fruiting bodies with this disease susceptibility grade (pieces); Z is the total number of Ganoderma lucidum fruiting bodies investigated in the plot. In this test example, Z = 60; B is the highest disease grade of the diseased Ganoderma lucidum in this plot.

[0102] Table 1

[0103]

[0104] In Table 1, "+" represents antagonism, and "-" represents no antagonism;

[0105] The letters a and b indicate that the difference is significant at the 0.05 level;

[0106] "HR" indicates high resistance; "HS" indicates high susceptibility.

[0107] During the stage of inoculating the pathogen on the fruiting body, most of the fruiting bodies of Ganoderma lucidum strain GZ206 did not show obvious symptoms after inoculating with Botrytis myophilum Figure 2 A), and the incidence rate was only 8.33%. "Ganoderma lucidum 78" formed obvious white flocculent aerial hyphae, which gradually covered the entire lower surface of the mushroom cap, and the diseased area > 70%, and the incidence rate was 94% Figure 2 B). The disease index of Ganoderma lucidum strain GZ206 was 8.33, and the disease index of "Ganoderma lucidum 78" was 60.20. According to the evaluation criteria for disease resistance and susceptibility, it can be known that Ganoderma lucidum strain GZ206 is a highly resistant strain, and "Ganoderma lucidum 78" is a highly susceptible strain.

[0108] It can be seen therefrom that the embodiment of the present invention provides a Ganoderma lucidum strain with high resistance to cobweb disease during the whole growth period, which helps to reduce the occurrence degree and epidemic speed of cobweb disease, is suitable for large-scale cultivation, and can generate greater economic and social benefits.

[0109] The embodiments described in the above exemplary embodiments do not represent all embodiments consistent with the present invention. On the contrary, they are merely examples of devices and methods consistent with some aspects of the present invention as detailed in the appended claims. The terms "including", "comprising" or any other variant thereof are intended to cover non-exclusive inclusion, so that a process, method, product or device including a series of elements not only includes those elements, but also includes other elements not expressly listed, or elements inherent to such process, method, product or device. Without further limitation, there is no exclusion of additional identical or equivalent elements in the process, method, product or device including the said elements.

[0110] The technical features of the above embodiments can be combined arbitrarily. For the sake of brevity of description, not all possible combinations of the technical features in the above embodiments are described. However, as long as there is no contradiction in the combination of these technical features, it should be considered as the scope described in this specification.

[0111] The above embodiments only express several implementation manners of the present invention, and their descriptions are relatively specific and detailed, but they should not be construed as limiting the scope of the invention patent. It should be noted that for those of ordinary skill in the art, without departing from the concept of the present invention, several modifications and improvements can still be made, and these all belong to the protection scope of the present invention. Therefore, the protection scope of the invention patent should be subject to the appended claims.

Claims

1. A Ganoderma lucidum strain GZ206 with high resistance to cobweb disease throughout the growth period, and its preservation number is CCTCC No: M 20221337.

2. Use of the Ganoderma lucidum strain GZ206 with high resistance to cobweb disease throughout the growth period as described in claim 1 in breeding, wherein the breeding is to select a strain highly resistant to the cobweb disease pathogen Cladobotryum mycophilum.

3. A Ganoderma lucidum mycelium, characterized in that The Ganoderma lucidum mycelium is obtained by culturing the Ganoderma lucidum strain (Ganoderma lucidum) GZ206 with high resistance to cobweb disease during the whole growth period as described in claim 1.

4. A Ganoderma lucidum fruiting body, characterized in that The Ganoderma lucidum fruit body is obtained by cultivating the Ganoderma lucidum mycelium as described in claim 3.

5. A cultivation method of the Ganoderma lucidum fruiting body as described in claim 4, characterized in that It includes the following steps: Providing a liquid spawn and / or a solid spawn including the Ganoderma lucidum mycelium as described in claim 3; Inoculating the liquid spawn and / or the solid spawn into a spawn bag for cultivation; After the mycelium fills the bag, continuing to place the spawn bag with the mycelium for after-ripening; And Moving the after-ripened spawn bag to light for fruiting cultivation to obtain the Ganoderma lucidum fruit body.

6. According to the cultivation method of the Ganoderma lucidum fruiting body as described in claim 5, characterized in that In the step of fruiting cultivation, the relative humidity is 75%-90%, and the daily lighting time is 8 h-14 h.

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