A specimen collection system and method for adapting a cryostat

Abstract

The application discloses a slice collecting system and a slice collecting method suitable for a freezing microtome. The slice collecting system comprises a slice pressing module. The slice pressing module cooperates with a blade of the freezing microtome to cut a frozen sample, so as to form a frozen tissue slice between the slice pressing module and the blade. The slice collecting system further comprises a releasing roller, a winding roller and an adhesive tape. The adhesive tape is movably arranged around the releasing roller, the slice pressing module and the winding roller. The adhesive tape arranged around the slice pressing module is located between the slice pressing module and the blade. An included angle between the adhesive tape released by the releasing roller and the adhesive tape wound by the winding roller is θ2, and θ2 is greater than or equal to 45 degrees. The included angle θ2 greater than or equal to 45 degrees can realize good adhesion between the adhesive tape around the slice pressing module and the frozen tissue slice, avoid the adhesive tape from being pulled due to too large turning angle during the slice collecting, and finally realize automatic collection of the slice through winding of the winding roller. The success rate and quality of the slice collecting are higher.

Description

Technical Field

[0001] This invention belongs to the field of biomedicine, and more specifically, relates to a slide receiving system and method adapted to a cryostat. Background Technology

[0002] Cryosectioning is a method and technique that involves freezing tissue at low temperatures to achieve a certain degree of hardness, followed by sectioning. Because it can preserve the immunomodulatory activity of various antigens relatively well, it is widely used in clinical pathology diagnosis and disease-related scientific research. Therefore, cryostats are among the most commonly used pieces of equipment in hospitals and research institutions. In the biomedical field, several domestic and international manufacturers have developed and produced various models of cryostats to meet clinical and research needs. The cutting platform in these cryostats typically includes a cutting tool and a slide compression module designed to work together. When the frozen sample reciprocates relative to the cutting tool under the drive of the motion mechanism, it can form thin sections, i.e., "frozen tissue sections," with the assistance of the slide compression module.

[0003] However, regardless of the model of cryostat, the frozen tissue sections obtained from cutting are scattered on the cutting platform. Operators can only manually collect these frozen tissue sections after the frozen sample cutting is completed or the cutting is paused. This undoubtedly reduces work efficiency, and the manual collection method will also affect the success rate of collection and may even damage the sections. Summary of the Invention

[0004] In view of the above-mentioned defects or improvement needs of the existing technology, the present invention provides a slide receiving system and slide receiving method adapted to a cryostat, the purpose of which is to solve the slide receiving problem of the existing cryostat.

[0005] To achieve the above objectives, according to one aspect of the present invention, a slide receiving system adapted to a cryostat is provided. The slide receiving system includes a pressing module that cooperates with the blade of the cryostat to cut frozen samples to form frozen tissue sections between the pressing module and the blade. The slide receiving system also includes an unwinding roller, a winding roller, and a tape. The tape is movably wound around the unwinding roller, the pressing module, and the winding roller, and the tape wound around the pressing module is located between the pressing module and the blade.

[0006] The angle between the tape released by the unwinding roller and the tape wound by the winding roller is θ2, where θ2 ≥ 45°.

[0007] Through the above technical solution, the adhesive tape is released from the unwinding roller, then passes through the compression module of the cryostat, where it adheres to the cut frozen tissue sections before being wound back into the take-up roller, thus completing the automatic collection of frozen tissue sections. Ensuring that the angle between the adhesive tape released from the unwinding roller and the tape wound by the take-up roller is no less than 45° ensures good adhesion and collection of the adhesive tape and frozen tissue sections at the compression module, regardless of the cryostat used. This avoids excessively large turning angles of the adhesive tape at the compression module, which could cause pulling on the frozen tissue sections adhered to the tape. After collection, the sections are wound back by the take-up roller, achieving automatic section collection. Compared to manual section collection, this method is more efficient and maintains the morphology of the frozen tissue sections better.

[0008] This invention also proposes a slide collection method adapted to a cryostat, based on the above-mentioned slide collection system, comprising the following steps:

[0009] Adjust the horizontal distance L1 between the take-up roller and the pressing module, and the height H1 of the take-up roller;

[0010] Adjust the horizontal distance L2 between the unwinding roller and the take-up roller;

[0011] Adjust the height H2 of the unwinding roller so that the included angle θ2 between the tape released by the unwinding roller and the tape wound by the take-up roller is ≥45°.

[0012] In the above technical solution, first adjust the horizontal position and height of the take-up roller, then adjust the distance between the take-up roller and the unwind roller, and finally adjust the horizontal position and height of the unwind roller. This allows θ2 to gradually increase. When adjusting the height of the unwind roller, it will not affect the adjustment results of the first two steps. Therefore, θ2≥45° can be achieved more quickly without repeated adjustments, making the adaptation operation simpler. Attached Figure Description

[0013] Figure 1 This is a schematic diagram showing the status of the slide receiving system when it is adapted to a cryostat.

[0014] Figure 2 This is a schematic diagram of the overall structure of the film receiving system;

[0015] Figure 3 This is a schematic diagram of the cooperation structure between the tablet compression module and the tablet slicing module in the tablet receiving system;

[0016] Figure 4 This is a schematic diagram of the cooperation structure between the tableting module and the slicing module in a cryostat in the prior art;

[0017] Figure 5 This is a schematic diagram of the structural position of the receiving system when H1 = H0;

[0018] Figure 6This is a schematic diagram of the structural position of the receiving system when H1 > H0.

[0019] In the diagram, 1. Take-up roller; 2. Unwind roller; 3. Belt; 4. Tableting module; 41. Adjusting frame; 42. Connecting frame; 43. Tableting roller; 44. Flexible block; 45. Flexible sheet; 46. Adjusting bolt; 5. Frozen sample; 6. Base; 7. Lifting mechanism; 8. Mounting hole; 9. Roll bottom; 10. Roll cover; 11. Stepped shaft; 12. Thread speed limiter; 131. Tool holder; 132. Blade; 14. Mounting bolt; 15. Waist-shaped groove. Detailed Implementation

[0020] To make the objectives, technical solutions, and advantages of this invention clearer, the invention will be further described in detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative and not intended to limit the invention. Furthermore, the technical features involved in the various embodiments of this invention described below can be combined with each other as long as they do not conflict with each other.

[0021] This invention discloses a slide receiving system adapted to a cryostat, such as... Figure 1 As shown, the slide receiving system includes a tablet compression module 4, which is located near the cutting edge of the blade 132 of the cryostat. The two work together to cut the frozen sample 5, forming a frozen tissue slice between the tablet compression module 4 and the blade 132. During cutting, the frozen sample 5 moves in a direction perpendicular to the tablet compression module 4, while the blade 132 remains stationary. Therefore, relative movement occurs between the frozen sample 5 and the blade 132, completing the cutting action. The frozen tissue slice cut by the blade 132 enters the space between the tablet compression module 4 and the blade 132, forming a flattened frozen tissue slice. The slide receiving system also includes an unwinding roller 2, a take-up roller 1, and a tape 3. The tape 3 is movably wound around the unwinding roller 2, the tablet compression module 4, and the take-up roller 1, and the tape 3 wound around the tablet compression module 4 is located between the tablet compression module 4 and the blade 132.

[0022] The angle between the tape released by the unwinding roller 2 and the tape 3 wound by the winding roller 1 is θ2, where θ2 ≥ 45°.

[0023] In this slide collection system, tape 3 is released from unwind roller 2 and then passes through the compression module 4 of the cryostat. Since the cut frozen tissue slides enter between the compression module 4 and the blade 132, the tape between the compression module 4 and the blade 132 adheres to the cut frozen tissue slides at the compression module 4 before being wound into the take-up roller 1, thus completing the automatic collection of the frozen tissue slides. The tape released from unwind roller 2 (i.e., the tape between unwind roller 2 and compression module 4) is the first tape, and the tape wound by take-up roller 1 (i.e., the tape between take-up roller 1 and compression module 4) is the second tape. The first and second tapes are wound around the compression module 4. When θ2 is too small, i.e., the turning angle between the first and second tapes is too large, the adhered frozen tissue slides will be pulled and damaged by tape 3. In cryostats from different manufacturers and models, the height and angle of the pressing module 4 are different. By ensuring that the included angle θ2 between the tape released by the unwinding roller 2 and the tape 3 wound by the winding roller 1 is ≥45°, it can be guaranteed that the turning angle from the first tape to the second tape will not be too large. The tape wrapped around the pressing module 4 can better adhere to the frozen tissue section and complete the adhesion.

[0024] Furthermore, the height of the unwinding roller 2 is H2, the horizontal distance between the take-up roller 1 and the unwinding roller 2 is L2, and the horizontal distance between the take-up roller 1 and the pressing module 4 is L1. H2, L1, and L2 are adjustable settings. Where H0 represents the height of the tableting module, and β represents the angle between the tableting module and the horizontal plane. The installation height and angle of the tableting module 4 vary among different manufacturers and models of cryostats. To adapt to different cryostats, H2, L1, and L2 are set to be adjustable. Therefore, the angle between the unwound tape 3 and the horizontal plane... This allows for adaptive adjustments based on different cryostats to meet the condition of ≥30°+β. In each cryostat, the height and position of the tableting module 4 are known, i.e., H0 and β are known; the horizontal distance L1 between the take-up roller 1 and the tableting module 4, the height H2 of the unwind roller, and the horizontal distance L2 between the unwind roller 2 and the take-up roller 1 can all be obtained through measurement. The angle between the tape 3 (first tape) released by the unwind roller 2 and the horizontal plane is... Control by adjusting H2, L1, and L2 This design ensures that the angle between the first adhesive tape and the horizontal plane is not too small. While maintaining a turning angle of at least 45° between the first and second adhesive tapes, it further aligns the first adhesive tape closer to the feed direction of the frozen sample, thus guaranteeing better adhesion of the frozen tissue sections during the mounting and collection process. Furthermore, no additional sensors are required for feedback control during adjustment. The entire collection system has a simple structure, is easy to install, disassemble, and transfer, and can be flexibly separated and reassembled with different cryostats.

[0025] Furthermore, the height of the take-up roller 1 is H1, and H1 is an adjustable setting. The angle between the tape 3 (second tape) wound by the take-up roller 1 and the horizontal plane is . Since the distance between the tablet compression module 4 and the blade 132 is at the micrometer level, and the cut frozen tissue slices are also at the micrometer level, when the angle is negative, the end of the second tape near the take-up roller 1 will come into contact with the blade 132, causing the frozen tissue slices to scrape against the end of the blade 132 near the take-up roller 1, resulting in the inability to take the slices back. Therefore, by adjusting H1... This allows the second tape to be positioned as close to the horizontal plane as possible, achieving a more ideal tape-gathering effect.

[0026] Since the height and position of the tablet compression module 4 within the cryostat are known, to more precisely control θ2 ≥ 45°, it is possible to... That is, by directly setting H1 = H0 during the adjustment process, there is no need to measure H1, thus avoiding measurement errors. Furthermore, theoretically, the larger θ2 is, the smaller the turning angle between the first and second tapes, and the less impact the adhesive force during slide collection has on the frozen tissue sections. To maximize the collection effect, it is also possible to... To obtain a larger θ2.

[0027] like Figure 2As shown, both the take-up roller 1 and the unwind roller 2 are connected to a lifting mechanism 7 at their bottom. The take-up system also includes a base 6, and the lifting mechanism 7 is detachably mounted on the upper surface of the base 6. The lifting mechanism 7 can be a Z-axis translation stage, a cylinder, etc., as long as the structure can achieve the lifting function. The two lifting mechanisms 7 are installed at different positions on the base 6, that is, the take-up roller 1 and the unwind roller 2 are set at different positions on the base 6. The base 6 can have multiple mounting holes 8, and the lifting mechanism 7 is fixed in the mounting holes 8 with screws. The adjustment of H1 and H2 is achieved by adjusting the two lifting mechanisms 7, and the adjustment of L2 is achieved by adjusting the installation position of the lifting mechanism 7 below the unwind roller 2. During use, the take-up roller 1, the unwind roller 2, and the pressing module 4 are located in the same vertical plane. Therefore, installing the lifting mechanism 7 below the unwind roller 2 closer to the take-up roller 1 will decrease L2, and installing the lifting mechanism 7 below the unwind roller 2 away from the take-up roller 1 will increase L2.

[0028] Specifically, since both the take-up roller 1 and the unwind roller 2 are used to wind the tape 3, they each include a bottom roll 9 and a top roll 10 that are parallel to each other. During the take-up and unwinding process, the tape 3 can be clamped between the bottom roll 9 and the top roll 10 for positioning and to prevent skewing. L2 is at its minimum when the take-up roller 1 and the unwind roller 2 approach each other until they contact each other. In some embodiments, such as... Figure 1 As shown, the winding speed of the take-up roller 1 is V1, and the cutting speed of the cryostat is V2, where V1 = V2. When the above conditions are met, the winding speed of the tape 3 is equal to the cutting speed. Therefore, this take-up method can be carried out synchronously with the cutting action. While the frozen sample 5 is being fed and cut, the tape 3 at the tableting module 4 is simultaneously wound up. The tape 3 and the cut frozen tissue slices move synchronously, thereby achieving take-up while cutting, without waiting for the cutting to stop. Furthermore, when V1 = V2, there will be no relative displacement between the tape 3 and the frozen tissue slices during adhesion, which can also prevent the tape 3 from pulling or damaging the frozen tissue slices.

[0029] Specifically, the take-up roller 1 is connected to a motor (not shown in the figure) via a coupling (not shown in the figure). The motor drives the take-up roller 1 to move actively, thereby taking up the tape 3. The unwind roller 2 is passively unwound under the action of the take-up roller 1. An encoder is installed in the motor, and the winding speed V1 is controlled by the motor speed. Therefore, V1 can be made equal to V2 by adjusting the motor speed.

[0030] In some embodiments, such as Figure 2As shown, a stepped shaft 11 is provided at the rotation center of the unwinding roller 2. A threaded speed limiter 12 is installed on the stepped shaft 11. The threaded speed limiter 12 provides resistance to the unwinding roller 2, thereby adjusting the tension of the tape 3 when unwinding. This avoids both excessive tension causing the tape 3 to stick to the frozen tissue slices and pull them, and insufficient tension causing the tape 3 to loosen and thus fail to collect the frozen tissue slices completely.

[0031] like Figure 3 As shown, the cryostat also includes a blade holder 131, on which the blade 132 is mounted. The tablet pressing module 4 of the tablet receiving system of this application includes two adjusting frames 41, a connecting frame 42, a tablet pressing roller 43, and two fine-tuning mechanisms. The two adjusting frames 41 are symmetrically mounted on the blade holder 131, and the two fine-tuning mechanisms are respectively fixed inside the two adjusting frames 41. The tablet pressing roller 43 is fixed at the end of the connecting frame 42 near the blade 132, and the end of the connecting frame 42 away from the blade 132 is respectively fixed to the two fine-tuning mechanisms.

[0032] Specifically, the blade holder 131 is triangular in shape, and the blade 132 is installed at one corner of the blade holder 131. The tablet pressing roller 43 in the tablet pressing module 4 is adjacent to the blade 132. The blade 132 and the tablet pressing roller 43 cooperate to cut the frozen sample 5. The connecting frame 42 is integrally formed with the tablet pressing roller 43. The tablet pressing roller 43 is fixed to the fine-tuning mechanism through the connecting frame 42. The fine-tuning mechanism enables fine-tuning of the position of the tablet pressing roller 43.

[0033] Furthermore, the fine-tuning mechanism includes a flexible block 44 and flexible thin plates 45 vertically connected to both ends of the flexible block 44. The two flexible thin plates 45 are parallel to each other, and the ends away from the flexible block 44 are fixed inside the adjusting frame 41. The flexible block 44 is fixed to the end of the connecting frame 42 away from the blade 132. The adjusting frame 41 is internally threaded with adjusting bolts 46 that abut against the side wall of the flexible block 44, allowing the position of the flexible block 44 to be adjusted in the horizontal direction. Furthermore, a precision shim is provided at the mounting point between the connecting frame 42 and the blade holder 131 to adjust the mounting height of the connecting frame 42 on the blade holder 131.

[0034] The height of the tablet pressing roller 43 is adjusted by adjusting the mounting height of the connecting frame 42 on the blade holder 131 using precision shims. A single parallelogram structure is formed between the two flexible thin plates 45 and the flexible block 44. By rotating the adjusting bolt 46, the flexible block 44 is pushed to move in the horizontal direction. At the same time, the flexible thin plates 45 connected to both ends of the flexible block 44 deform, controlling the direction and distance of movement of the flexible block 44, achieving micron-level displacement of the flexible block 44, thereby adjusting the position of the tablet pressing roller 43 in the horizontal direction. Therefore, the tablet pressing roller 43 can achieve position adjustment in two dimensions, the height direction and the horizontal direction, to meet the cutting requirements of frozen tissue sections of different thicknesses. Because the tableting roller 43 and the blade 132 are relatively long, it is difficult to ensure that the distance between them is uniform. Therefore, the cut frozen tissue sections sometimes curl on one side, indicating that the distance between the tableting roller 43 and the blade 132 on that side is large. This application uses a symmetrical adjustment frame 41 and two fine-tuning mechanisms to reduce the distance between the tableting roller 43 and the blade 132 on the curled side by using a precision shim and fine-tuning mechanism on the curled side. This allows the tableting roller 43 to fit better against the surface of the frozen tissue section and avoids curling.

[0035] like Figure 4 As shown, in current cryostats, the tableting module 4 includes an adjusting frame 41, a connecting frame 42, and a tableting roller 43. It lacks a fine-tuning mechanism; the tableting roller 43 is integrally formed with the connecting frame 42. The adjusting frame 41 is fixed to the end of the connecting frame 42 away from the tableting roller 43. The adjusting frame 41 is then mounted on the blade holder 131 using mounting bolts 14 and a slotted groove 15. Horizontal adjustment of the tableting roller 43 is achieved by loosening the mounting bolts 14 to move the adjusting frame 41 horizontally, and then tightening the mounting bolts 14 to fix it. This method results in a large adjustment displacement, making micron-level fine-tuning impossible. Therefore, when using the tape 3 of this application for tablet collection, the distance between the tableting roller 43 and the blade 132 cannot be precisely controlled, making it difficult to achieve effective tablet collection using the tape 3. Figure 3 As shown, this application improves the tableting module 4 and the slicing module in the existing cryostat by using a fine-tuning mechanism composed of a flexible block 44 and two flexible thin plates 45 to connect the adjustment frame 41 and the connecting frame 42. The flexible block 44 can be adjusted at the micron level in the horizontal direction by the deformation of the flexible thin plates 45. Compared with the existing cryostat, it can achieve better slide collection effect with less modification.

[0036] This invention also proposes a slide collection method adapted to a cryostat, implemented based on the aforementioned slide collection system, such as... Figure 1 As shown, it includes the following steps:

[0037] S1, adjust the horizontal distance L1 between the take-up roller 1 and the pressing module 4 and the height H1 of the take-up roller 1;

[0038] S2, adjust the horizontal distance L2 between the unwinding roller and the take-up roller;

[0039] S3, adjust the height H2 of the unwinding roller 2 so that the included angle θ2 between the tape 3 released by the unwinding roller 2 and the tape 3 wound by the winding roller 1 is ≥45°.

[0040] First, adjust the height of the take-up roller 1 to fix H1. Then, adjust the distance between the unwind roller 2 and the take-up roller 1 to fix L2. Finally, adjust the height of the unwind roller 2. The adjustment process will not affect the adjustment results of the previous two steps. It can more conveniently and quickly make the included angle θ2 between the tape 3 (first tape) released by the unwind roller 2 and the tape 3 (second tape) wound by the take-up roller 1 ≥ 45°.

[0041] Furthermore, after the height and horizontal position of the take-up roller 1 are adjusted, they meet the requirements.

[0042] After the horizontal position and height of the unwinding roller 2 are adjusted, the following conditions are met:

[0043] because By adjusting S1, the angle between the second tape and the horizontal plane can be made as small as possible without affecting the take-up of the tape, and the angle between the first tape and the horizontal plane can be made as large as possible without affecting the take-up of the tape, so as to obtain the largest possible θ2, further reducing the turning angle between the first tape and the second tape and improving the take-up effect.

[0044] Preferably, when adjusting the height H1 of the take-up roller 1, H1 = H0;

[0045] When adjusting the horizontal distance L2 between the unwinding roller 2 and the take-up roller 1, move the unwinding roller 2 towards the take-up roller 1 to minimize L2.

[0046] By aligning the height of the take-up roller 1 with the height of the tableting module 4, the angle between the retracted tape 3 and the horizontal plane is 0°. The closer this angle is to 0°, the longer the adhesion distance between the frozen tissue section and the tape 3, which improves the take-up effect. Furthermore, since the height of the tableting module 4 can be directly obtained from the cryostat, there is no need for repeated measurements during the adjustment of H1, simplifying the adjustment steps and making the entire adjustment process more precise.

[0047] The smaller L2 is, the less tension the tape 3 loop exerts on the tableting module 4, which helps protect the structure of the tableting module 4 in the cryostat.

[0048] Preferably, when adjusting L1 in S1, the lifting mechanism 7 under the take-up roller 1 is installed on the edge of the base 6, and then the take-up roller 1 is placed close to the freezing slicer. The horizontal position of the take-up roller 1 is fixed and L1 is minimized. The smaller L1 is, the better. This allows for obtaining larger values, thereby further improving the image capture effect. Specifically, such as... Figure 5 As shown, S1 adjusts H1 by adjusting the lifting mechanism 7 under the take-up roller 1. As the tape 3 is wound and unwound, the thickness of the tape 3 on the take-up roller 1 and the unwound roller 2 changes, causing the actual H1 to change. Let the angle between the wound tape 3 (second tape) and the horizontal plane be θ1, the angle between the unwound tape 3 and the wound tape 3 be θ2, and the angle between the unwound tape 3 (first tape) and the feed direction of the frozen sample 5 be α. Then, θ1 + θ2 + α = 90° + β. The following analysis considers two scenarios: when H1 = H0. See Figure 6 When H1-H0>0, θ1>0°, Therefore, regardless of whether θ1 = 0° can be strictly achieved during actual operation, θ2 ≥ 45° can be achieved by controlling H1, H2, L1, and L2.

[0049] During the adjustment process of S2, the lifting mechanism 7 under the unwinding roller 2 can be moved closer to the take-up roller 1 until the unwinding roller 2 contacts the take-up roller 1. Then, the lifting mechanism 7 under the unwinding roller 2 is fixed on the base 6. This will minimize L2 (different sizes of the unwinding roller 2 and the take-up roller 1, as well as different thicknesses of the tape 3 wound on the unwinding roller 2 before unwinding, will result in different minimum values ​​of L2, so the S2 operation is required during adaptation). Furthermore, the horizontal distance L2 between the unwinding roller 2 and the take-up roller 1 can be obtained at any time before the start of the adjustment of H2. During the adjustment process of S2, the unwinding roller 2, the take-up roller 1, and the tablet pressing module 4 must be kept in the same vertical plane so that the tape 3 can move in a vertical plane without distorting, thus avoiding pulling on the frozen tissue slices.

[0050] Based on the above judgment process, since the specific L2 value can be obtained after S2 adjustment, and this value is related to the size of the take-up roller 1 and the unwind roller 2, it is a fixed and known parameter. Therefore, before adjusting the height H2 of the unwind roller 2, the specific H0, L1, L2, and β values ​​can be directly substituted into the above formula to obtain the specific judgment conditions.

[0051] For the system, this judgment method does not require additional installation of sensors, feedback control or other structures. For the operation method, the judgment process has low requirements for operational precision. Cryostats are usually used by doctors, nurses, researchers and others, and there is no need for strict operation guidance and training. It is more convenient and flexible when adapting to different cryostats.

[0052] The method for taking up the frozen sample 5 in this invention further includes step S4: reading the cutting speed V2 of the cryostat (i.e., the speed of the frozen sample 5 in the feed direction), and adjusting the winding speed of the take-up roller 1 to V1, so that V1 = V2. Since the cutting speed V2 of the cryostat is a known parameter and can be directly read from the device, only V1 needs to be adjusted during operation. The motor connected to the take-up roller 1 has a built-in speed controller; adjusting this controller allows for adjustment of the rotational speed of the take-up roller 1, i.e., adjustment of the winding speed V1.

[0053] In some embodiments, S4 also includes tension adjustment, that is, adjusting the thread speed limiter 12 on the unwinding roller 2 to make the tension of the tape 3 between 500N and 2000N. Regardless of the order of tension adjustment and speed adjustment, when the tension and speed are adjusted to meet the above requirements, the tape 3 can further improve the receiving effect of frozen tissue sections. Compared with manual receiving, it can not only be carried out simultaneously during cutting, saving time, but also better control the receiving force, avoiding damage to the frozen tissue sections.

[0054] Through steps S1-S4, the position, speed, and tension of the tape 3 can be adjusted to achieve synchronous collection of frozen tissue sections during the cutting process. Because the tableting roller 43 and blade 132 are relatively long, while the tape 3 is relatively narrow, it is difficult to ensure that the distance between the tableting roller 43 and blade 132 is uniform throughout, which can lead to curling of one side of the frozen tissue section. Alternatively, due to the varying cutting thickness of the frozen tissue sections under different usage conditions, when the distance between the tableting roller 43 and blade 132 is greater than the cutting thickness of the frozen tissue section, curling of the entire frozen tissue section can also easily occur. Therefore, in some embodiments, step S5 is also included, rotating the adjusting bolt 45 of the tableting module 4 within the collection system of this application to adjust the position of the tableting roller 43 via a fine-tuning mechanism. The thickness of frozen tissue sections is typically only at the micrometer level; adjusting the position of the tableting roller 43 at the micrometer level through the fine-tuning mechanism allows for better coordination between the tableting roller 43 and blade 132, resulting in better cutting and collection effects.

[0055] Those skilled in the art will readily understand that the above description is merely a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, and improvements made within the spirit and principles of the present invention should be included within the scope of protection of the present invention.

Claims

1. An adaptation of a section collection system of a cryostat, characterized in that, The collecting system comprises a tabletting module cooperating with the blade of the freezing microtome to cut the frozen sample to form a frozen tissue slice between the tabletting module and the blade, and a releasing roller, a collecting roller and a tape, the tape being movably arranged on the releasing roller, the tabletting module and the collecting roller, and the tape arranged at the tabletting module being located between the tabletting module and the blade. An included angle between the adhesive tape discharged by the unwinding roller and the adhesive tape wound by the winding roller is θ2, .

2. The sheet collection system according to claim 1, characterized by The unwinding roller has a height H2, the horizontal distance between the winding roller and the unwinding roller is L2, the horizontal distance between the winding roller and the tabletting module is L1, H2, L1, L2 are adjustable settings, wherein H0 represents the height of the tabletting module, and β represents the angle between the tabletting module and the horizontal plane.

3. The sheet collection system according to claim 2, wherein The height of the winding roller is H1, H1 is adjustable setting, .

4. The sheet collection system according to claim 2, wherein The collecting speed of the collecting roller is V1, and the cutting speed of the freezing microtome is V2, V1 = V2.

5. The system of claim 2, the microtome further comprising a knife holder, the blade mounted on the knife holder, wherein, The tabletting module comprises two adjusting frames, a connecting frame, a tabletting roller and two fine adjustment mechanisms, the two adjusting frames are symmetrically installed on the blade holder, the two fine adjustment mechanisms are respectively fixed in the two adjusting frames, the tabletting roller is fixed at one end of the connecting frame close to the blade, and the other end of the connecting frame away from the blade is respectively fixed with the two fine adjustment mechanisms.

6. The sheet collection system according to claim 5, wherein The fine adjustment mechanism comprises a flexible block and a flexible sheet vertically connected to both ends of the flexible block, the flexible block is fixed at one end of the connecting frame away from the blade, the two flexible sheets are parallel to each other and fixed at the other end away from the flexible block in the adjusting frame, and the adjusting screw threadedly connected to the side wall of the flexible block is arranged on the adjusting frame.

7. A method of adapting a method of sectioning for a cryostat, implemented on the basis of the sectioning system according to claim 3, characterized in that, The method comprises the following steps: Adjusting the horizontal distance L1 between the collecting roller and the tabletting module and the height H1 of the collecting roller; Adjusting the horizontal distance L2 between the releasing roller and the collecting roller; adjusting the height H2 of the unwinding roller so that the included angle between the adhesive tape unwound by the unwinding roller and the adhesive tape wound by the winding roller .

8. The sheet collecting method according to claim 7, wherein The height and horizontal position of the winding roller are adjusted to meet ; After the horizontal position and height of the unwinding roller are adjusted, the following conditions are met .

9. The sheet collecting method according to claim 8, wherein When adjusting the height H1 of the collecting roller, H1 = H0; When adjusting the horizontal distance L2 between the releasing roller and the collecting roller, the releasing roller is moved towards the collecting roller to make L2 minimum.

10. The sheet collecting method according to claim 9, wherein Further comprising: Reading the cutting speed V2 of the freezing microtome, adjusting the collecting speed V1 of the collecting roller to make V1 = V2.

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