Microbial photoelectrochemical characterization apparatus and methods of use

Abstract

The application discloses a kind of microbial photoelectrochemical characterization device and use method, microbial photoelectrochemical characterization device includes: the support with scanning station;Multi-cavity sample cell, rotatably arranged on support, including transparent base electrode and side wall structure, side wall structure and base electrode are enclosed to form multiple annular distribution containing cavities;Illumination device, arranged on support and used to provide illumination;Scanning electrochemical microscope device, including three-dimensional controller, electrochemical workstation, ultramicroelectrode, reference electrode and counter electrode;Cover, cover is arranged on the top of multi-cavity sample cell, cover is correspondingly provided with first through-hole for passing ultramicroelectrode, reference electrode and counter electrode on each containing cavity;Gas supply device, for providing nitrogen;Driving device, for driving multi-cavity sample cell rotation.The structure can make sample in the process of scanning test in anaerobic state, and scanning test can also be carried out to multiple samples.

Description

TECHNICAL FIELD

[0001] The present application relates to the field of microbial photoelectrochemistry, in particular to a microbial photoelectrochemistry characterization device and a use method thereof. BACKGROUND

[0002] The microbial photoelectrochemistry system is also called a microbial hybrid system, mainly including two key components of a photosensitizer and a microbial catalyst. The basic working principle of the microbial photoelectrochemistry system is as follows: under the illumination of a specific wavelength, when the photon energy absorbed by the photosensitizer is equal to or higher than the band gap width thereof, the electrons (e-) on the valence band are excited to jump to the conduction band, thereby generating photoelectrons with higher energy. Subsequently, the photoelectrons can be transmitted to the outer membrane active site of the microbial catalyst through direct transmission, redox mediators or other ways, and then drive intracellular energy metabolism activities.

[0003] The fine characterization of the process of "generation-transmission-capture" of photoelectrons is the basis and key to interpreting the macro effect and micro mechanism of the microbial photoelectrochemistry system. However, the characterization methods and means currently adopted are mostly based on simple bioelectrochemical responses, and it is difficult to achieve scientific quantification and accurate analysis, which is due to the following reasons: on the one hand, photoelectrons are fleeting and easy to recombine with holes, and signal capture is difficult; on the other hand, the outer membrane protein of the microorganism usually contains a complex electron transfer chain such as cytochrome and hydrogenase, and the extracellular utilization of photoelectrons mediated by the electron transfer chain is a multi-pathway cooperative microbial / extracellular donor interface electron transfer process, and the utilization efficiency is usually affected by multiple factors. Although the existing technology discloses a photoelectrochemical kinetics test system based on a scanning electrochemical microscope, the system is mainly suitable for aerobic photoelectrochemical processes of non-biological organisms, and is not suitable for anaerobic photoelectrochemical processes involving microorganisms. In addition, the system cannot realize continuous and automatic scanning test on multiple samples.

[0004] Therefore, how to accurately, in-situ and in real time characterize the transmission process of photoelectrons generated by the photosensitizer to the outer membrane active site of the microbial catalyst is a great research difficulty in the field of microbial photoelectrochemistry. SUMMARY

[0005] The present application aims to at least solve one of the technical problems existing in the prior art. To this end, the present application provides a microbial photoelectrochemistry characterization device, which can keep the sample in an anaerobic state during the scanning test, thereby realizing the characterization of the anaerobic photoelectrochemical process involving microorganisms, filling the gap in the related art, and also being capable of scanning test on multiple samples.

[0006] The present application also provides a use method applied to the microbial photoelectrochemistry characterization device.

[0007] The microbial photoelectrochemistry characterization device according to the first aspect of the present application comprises:

[0008] a support, wherein a scanning station is arranged on the support;

[0009] a multi-cavity sample pool, which is rotatably arranged on the support, wherein the multi-cavity sample pool comprises a transparent base electrode and a side wall structure arranged on the base electrode, and the side wall structure and the base electrode enclose a plurality of annularly distributed containing cavities for containing samples;

[0010] an illumination device, which is arranged on the support and capable of providing illumination for the containing cavities;

[0011] a scanning electrochemical microscope device, which comprises a three-dimensional controller, an electrochemical workstation, an ultramicroelectrode, a reference electrode and a counter electrode, wherein the three-dimensional controller is used to control the ultramicroelectrode, the reference electrode and the counter electrode to scan samples in the containing cavities located in the scanning station, and the electrochemical workstation is used to connect the ultramicroelectrode, the reference electrode and the counter electrode and collect electrochemical information generated by scanning samples;

[0012] a cover, which is arranged on the top of the multi-cavity sample pool, and a first through hole for the ultramicroelectrode, the reference electrode and the counter electrode is arranged on the cover corresponding to each containing cavity;

[0013] a gas supply device, which is arranged on the support and used to provide nitrogen to the containing cavities;

[0014] a driving device, which is arranged on the support and used to drive the multi-cavity sample pool to rotate, so that different containing cavities can be rotated to the scanning station in turn.

[0015] The microorganism photoelectrochemical characterization device according to the embodiment of the present application has at least the following beneficial effects: nitrogen is provided to the containing cavities by the gas supply device, so that the samples can be in an anaerobic state during scanning test, and the characterization of the anaerobic photoelectrochemical process in which microorganisms participate is realized, which fills the gap in the related art. In addition, the multi-cavity sample pool has a plurality of containing cavities that can be rotated to the scanning station, so that a plurality of samples can be scanned and tested. The base electrode is used as the bottom structure of the multi-cavity sample pool, which overcomes the limitation of the traditional photoelectrochemical kinetics test system that the working electrode area of the base electrode is small and the sample needs to be pretreated by electrochemical deposition technology. In addition, the plurality of containing cavities share the same base electrode, which can maximize the consistency of the test conditions of the plurality of containing cavities, thereby reducing the test error caused by the difference in experimental conditions.

[0016] According to some embodiments of the present application, the illumination device comprises a light source and an on-off device, the on-off device is arranged on the support and below the multi-cavity sample cell, the light source is arranged on the support and below the on-off device, the light source is used to provide illumination for the accommodation cavities, and the on-off device can control the on-off of the light path between the accommodation cavities on the scanning station and the light source.

[0017] According to some embodiments of the present application, the on-off device comprises a base and a first baffle, the base is fixedly arranged at the bottom of the base electrode, the base is provided with a second through hole for the light path corresponding to each accommodation cavity, and the side edge of the base is provided with a insertion hole communicated with the second through hole, the first baffle is movably arranged in the insertion hole and can block the light path passing through the second through hole.

[0018] According to some embodiments of the present application, the support is provided with a supporting seat corresponding to the base, the supporting seat is provided with an annular groove, and the annular groove is provided with a steel ball for supporting the base.

[0019] According to some embodiments of the present application, the driving device comprises a motor, a rotating seat, a first bevel gear, a second bevel gear and a control device, the motor is arranged on the support and electrically connected with the control device, the upper end of the rotating seat is fixedly connected with the base electrode or the base, the first bevel gear is fixedly arranged at the lower end of the rotating seat, the second bevel gear is arranged at the output end of the motor and meshed with the first bevel gear, so that the motor can drive the rotating seat to rotate, thereby driving the multi-cavity sample cell to rotate, and the control device is used to control the motor, so that different accommodation cavities can be automatically rotated to the scanning station in sequence.

[0020] According to some embodiments of the present application, the gas supply device comprises an air inlet pipeline, a gas supply main pipe, a gas supply branch pipe and a rotary joint, one end of the air inlet pipeline is used to be connected with a nitrogen gas source, the other end of the air inlet pipeline is connected with the lower end of the gas supply main pipe through the rotary joint, so that the gas supply main pipe can rotate relative to the air inlet pipeline, the upper end of the gas supply main pipe is connected with a plurality of gas supply branch pipes corresponding to a plurality of accommodation cavities in one-to-one correspondence, and a plurality of gas supply branch pipes are communicated with a plurality of accommodation cavities in one-to-one correspondence.

[0021] According to some embodiments of the present application, the first through hole is provided with a flexible blocking piece, and the flexible blocking piece is provided with a third through hole.

[0022] According to some embodiments of the present application, the lower end of the first through hole is provided with an extension pipe body, the lower end opening of the extension pipe body is provided with a second shutter capable of being opened and closed, and a torsion spring for driving the second shutter to close is arranged between the second shutter and the extension pipe body.

[0023] According to some embodiments of the present application, the base electrode is one of FTO conductive glass and ITO conductive glass.

[0024] The use method of the second aspect of the present application is applied to the microorganism photoelectrochemical characterization device of the first aspect of the present application, and comprises the following steps:

[0025] An electrolyte solution is prepared by selecting a sterilized microorganism culture medium and an oxidation-reduction substance, and is placed in each accommodation cavity;

[0026] Different samples are respectively placed in different accommodation cavities;

[0027] Nitrogen is continuously supplied to the accommodation cavities by the gas supply device;

[0028] The ultramicro electrode, the reference electrode and the counter electrode are fixed on the three-dimensional controller, and the ultramicro electrode, the reference electrode and the counter electrode are connected to the electrochemical workstation;

[0029] The light illumination device is turned on to provide light illumination for the accommodation cavities located at the scanning station;

[0030] The ultramicro electrode, the reference electrode and the counter electrode are inserted into the accommodation cavities located at the scanning station by the three-dimensional controller, and the ultramicro electrode is driven to move to a preset position to complete positioning of the ultramicro electrode and drawing of an approaching curve;

[0031] The ultramicro electrode, the reference electrode and the counter electrode are controlled to scan the sample in the accommodation cavity located at the scanning station by the three-dimensional controller, then the ultramicro electrode, the reference electrode and the counter electrode are lifted above the multi-cavity sample pool by the three-dimensional controller, then the multi-cavity sample pool is driven to rotate by the driving device, so that the next accommodation cavity is rotated to the scanning station and the sample in the accommodation cavity is scanned according to the above steps, and the scanning is repeated until the scanning of the samples in all the accommodation cavities is completed;

[0032] After a preset time interval, the samples in all the accommodation cavities are scanned again according to the above steps.

[0033] Additional aspects and advantages of the present application will be made apparent from the following description. BRIEF DESCRIPTION OF DRAWINGS

[0034] The above and / or additional aspects and advantages of the present application will become apparent and more readily appreciated from the following description of the embodiments, taken in conjunction with the accompanying drawings in which:

[0035] Figure 1 is a structural schematic diagram of a microbial photoelectrochemical characterization device of an embodiment of the present application;

[0036] Figure 2 is a partial structural schematic diagram of the structure shown in Figure 1

[0037] Figure 3

[0038] Figure 4 is a cross-sectional view of A-A in Figure 3

[0039] Figure 5 Figure 4

[0040] Figure 6 Figure 4

[0041] Figure 7 Figure 4

[0042] Figure 8

[0043] Figure 9

[0044] Figure 10

[0045] Figure 11 Figure 10

[0046] Reference Signs:

[0047] ​​​​​​​​​​​​​​Support 100, multi-cavity sample cell 200, base electrode 210, second escape hole 211, side wall structure 220, containing cavity 230, light source 310, on-off device 320, base 321, second through hole 3211, jack 3212, first escape hole 3213, first baffle 322, handle 3221, three-dimensional controller 410, electrochemical workstation 420, ultramicroelectrode 430, reference electrode 440, counter electrode 450, cover 500, flexible barrier 510, third through hole 511, extension pipe body 520, second baffle 530, torsional spring 540, air inlet pipeline 610, air supply main pipe 620, air supply branch pipe 630, rotary joint 640, motor 710, rotating seat 720, escape channel 721, first bevel gear 730, second bevel gear 740, bearing 750, bearing seat 800, annular groove 810, steel ball 820. DETAILED DESCRIPTION

[0048] Embodiments of the present application are described below in detail, examples of which are shown in the drawings, wherein the same or similar reference signs represent the same or similar elements or elements having the same or similar functions throughout. The embodiments described below by referring to the drawings are exemplary and are only used to explain the present application, and cannot be understood as a limitation of the present application.

[0049] In the description of the present application, it should be understood that, if the orientation description is involved, for example, the orientation or position relationship indicated by the upper, lower, front, rear, left, right, etc. is based on the orientation or position relationship shown in the drawings, which is only for the convenience of describing the present application and simplifying the description, and therefore cannot be understood as a limitation of the present application, which does not indicate or imply that the device or element indicated must have a particular orientation, be constructed and operated in a particular orientation.

[0050] In the description of the present application, if the words such as several, greater than, less than, more than, above, below, within, etc. appear, wherein the meaning of several is one or more, the meaning of multiple is two or more, greater than, less than, more than, etc. are understood as not including the number, and above, below, within, etc. are understood as including the number.

[0051] In the description of the present application, if the words such as first, second, etc. appear, they are only used for the purpose of distinguishing technical features, and cannot be understood as indicating or implying relative importance or implicitly indicating the number of indicated technical features or implicitly indicating the sequence of indicated technical features.

[0052] In the description of the present application, unless otherwise explicitly limited, the words such as setting, installing, connecting, etc. should be broadly understood, and the person skilled in the art can reasonably determine the specific meaning of the above words in the present application in combination with the specific content of the technical solution.

[0053] Reference Figures 1 to 11The microbial photoelectrochemical characterization device according to the embodiment of the application comprises a support 100, a multi-cavity sample pool 200, an illumination device, a scanning electrochemical microscope device, a cover 500, a gas supply device and a driving device.

[0054] The support 100 has a scanning station, the multi-cavity sample pool 200 is rotatably arranged on the support 100, the multi-cavity sample pool 200 comprises a transparent base electrode 210 and a side wall structure 220 arranged on the base electrode 210, the side wall structure 220 and the base electrode 210 enclose a plurality of annularly distributed containing cavities 230 for containing samples, specifically, the surface of the base electrode 210 is plated with a conductive coating, wherein the material of the conductive coating is one of nano-gold, nickel-gold and graphene, the illumination device is arranged on the support 100 and can provide illumination for the containing cavities 230, the scanning electrochemical microscope device comprises a three-dimensional control instrument 410, an electrochemical workstation 420, an ultramicroelectrode 430, a reference electrode 440 and a counter electrode 450, the three-dimensional control instrument 410 is used to control the ultramicroelectrode 430, the reference electrode 440 and the counter electrode 450 to scan the samples in the containing cavities 230 located in the scanning station, specifically, the three-dimensional control instrument 410 comprises an X-axis linear module, a Y-axis linear module and a Z-axis linear module, which can drive the ultramicroelectrode 430, the reference electrode 440 and the counter electrode 450 to move along the X-axis direction, the Y-axis direction and the Z-axis direction, the specific structure and working principle thereof are known in the art, and will not be described here, the electrochemical workstation 420 is used to connect the ultramicroelectrode 430, the reference electrode 440 and the counter electrode 450 and collect the electrochemical information generated by the scanned samples, the cover 500 is arranged on the top of the multi-cavity sample pool 200, the cover 500 is provided with a first through hole corresponding to each containing cavity 230 for penetrating the ultramicroelectrode 430, the reference electrode 440 and the counter electrode 450, the gas supply device is arranged on the support 100 and is used to supply nitrogen to the containing cavities 230, and the driving device is arranged on the support 100 and is used to drive the multi-cavity sample pool 200 to rotate, so that different containing cavities 230 can be rotated to the scanning station in turn.

[0055] The nitrogen gas is provided into the containing cavity 230 through the gas supply device, so that the sample can be in an anaerobic state during the scanning test, the characterization of the anaerobic photoelectrochemical process involving microorganisms is realized, the blank of the related art is filled, the multi-cavity sample pool 200 has multiple containing cavities 230 capable of rotating to the scanning station, so that multiple samples can be scanned and tested, the substrate electrode 210 is used as the bottom structure of the multi-cavity sample pool 200, the limitation that the working electrode area of the substrate electrode is small in the conventional photoelectrochemical kinetics test system and the sample needs to be pretreated by electrochemical deposition technology is overcome, in addition, the multiple containing cavities 230 share the same substrate electrode 210, the consistency of the test conditions of the multiple containing cavities 230 can be maximally ensured, and the test error caused by the difference of the experimental conditions can be reduced.

[0056] With reference to Figures 2 to 4 In some embodiments, the light irradiation device includes a light source 310 and an on-off device 320, the on-off device 320 is arranged on the support 100 and located below the multi-cavity sample pool 200, the light source 310 is arranged on the support 100 and located below the on-off device 320, the light source 310 is used for providing light irradiation into the containing cavity 230, and the on-off device 320 can control the on-off of the light path between the containing cavity 230 located at the scanning station and the light source 310, so that the light irradiation device can selectively provide light irradiation for the containing cavity 230 according to the requirement of the scanning test.

[0057] With reference to Figure 4 , Figure 6 and Figure 9In some embodiments, the on-off device 320 includes a base 321 and a first shutter 322. The base 321 is fixedly arranged at the bottom of the base electrode 210, so that the base 321 can rotate synchronously with the multi-cavity sample pool 200. The base 321 is provided with a second through hole 3211 for the light path corresponding to each holding cavity 230. The side edge of the base 321 is provided with a insertion hole 3212 connected to the second through hole 3211. The first shutter 322 is movably arranged in the insertion hole 3212 and can block the light path through the second through hole 3211. In use, the first shutter 322 is inserted into the insertion hole 3212 to block the light path through the second through hole 3211. The first shutter 322 is pulled out of the insertion hole 3212 to provide light for the corresponding holding cavity 230 by the light source 310. The first shutter 322 is provided with a handle 3221 for applying force, so as to facilitate the insertion and pulling of the first shutter 322. In addition, the support 100 is provided with an independent light source 310 corresponding to each holding cavity 230, which is beneficial to ensure the consistency of the lighting effect. Of course, the on-off device can also be replaced by a shutter structure driven by a push-pull electromagnet. Specifically, a shutter structure is arranged for each holding cavity 230 to control the on-off of the light path between the holding cavity 230 at the scanning station and the light source 310. The specific structure is not limited here.

[0058] With reference to Figure 4 and Figure 6 In some embodiments, the support 100 is provided with a supporting seat 800 corresponding to the base 321. The supporting seat 800 is provided with an annular groove 810. The annular groove 810 is provided with a steel ball 820 for supporting the base 321, which is beneficial to reduce the frictional resistance.

[0059] With reference to Figures 1 to 4 and Figure 7In some embodiments, the driving device comprises a motor 710, a rotating seat 720, a first bevel gear 730, a second bevel gear 740 and a control device. The motor 710 is arranged on the support 100 and electrically connected with the control device. The upper end of the rotating seat 720 is fixedly connected with the base electrode 210 or the base 321. The first bevel gear 730 is fixedly arranged at the lower end of the rotating seat 720. The second bevel gear 740 is arranged at the output end of the motor 710 and engaged with the first bevel gear 730, so that the motor 710 can drive the rotating seat 720 to rotate, thereby driving the multi-cavity sample cell 200 to rotate. The control device is used to control the motor 710, so that different accommodating cavities 230 can be automatically and sequentially rotated to the scanning station for scanning the samples therein. The lower part of the rotating seat 720 is sleeved with a bearing 750 fixedly connected with the support 100. The above-mentioned driving device can cooperate with the scanning electrochemical microscope device to perform real-time, continuous and automatic scanning test on multiple samples. Specifically, the control device is a time control switch. Of course, the control device can also be a PLC controller or a computer, which is not limited here.

[0060] With reference to Figures 2 to 4 And Figures 7 to 9 In some embodiments, the gas supply device comprises an air inlet pipeline 610, a gas supply main pipeline 620, a gas supply branch pipeline 630 and a rotary joint 640. One end of the air inlet pipeline 610 is connected with a nitrogen gas source. The other end of the air inlet pipeline 610 is connected with the lower end of the gas supply main pipeline 620 through the rotary joint 640, so that the gas supply main pipeline 620 can rotate relative to the air inlet pipeline 610. The upper end of the gas supply main pipeline 620 is connected with a plurality of gas supply branch pipelines 630 corresponding to the plurality of accommodating cavities 230. The plurality of gas supply branch pipelines 630 are communicated with the plurality of accommodating cavities 230 one by one. When the driving device drives the multi-cavity sample cell 200 to rotate, the gas supply branch pipeline 630 and the gas supply main pipeline 620 rotate together and do not drive the air inlet pipeline 610, so that the air inlet pipeline 610 can stably deliver nitrogen gas. Specifically, the upper end of the rotating seat 720 is provided with a first avoiding hole 3213 corresponding to the base 321. The upper end of the rotating seat 720 penetrates through the avoiding hole and is fixedly connected with the base electrode 210. The rotating seat 720 is provided with an avoiding channel 721 corresponding to the gas supply main pipeline 620. The bottom of the base electrode 210 is provided with a second avoiding hole 211 corresponding to the gas supply main pipeline 620. The upper part of the gas supply main pipeline 620 penetrates through the avoiding channel 721 and the second avoiding hole 211 and extends into the middle region surrounded by the plurality of accommodating cavities 230. The plurality of gas supply branch pipelines 630 are communicated with the plurality of accommodating cavities 230 one by one through the side wall structure 220, so that the gas supply main pipeline 620 and the gas supply branch pipeline 630 do not block the light path of the illumination device and do not interfere with the driving device. The upper end of the rotating seat 720 can also be fixedly connected with the base 321, which is not limited here.

[0061] With reference toFigures 2 to 5 In some embodiments, the first through hole is provided with a flexible barrier 510, the flexible barrier 510 is provided with a third through hole 511, and the flexible barrier 510 is deformed under external force, so that the flexible barrier 510 does not hinder the movement of the ultramicroelectrode 430, the reference electrode 440 and the counter electrode 450 in the horizontal direction, that is, the three-dimensional controller 410 does not hinder the scanning of the ultramicroelectrode 430, the reference electrode 440 and the counter electrode 450 in the sample in the holding cavity 230 at the scanning station, and at the same time, the flexible barrier 510 is beneficial to prevent oxygen from entering the holding cavity 230. Specifically, the flexible barrier 510 is soft rubber.

[0062] Referring to Figure 5 , Figure 10 and Figure 11 In some embodiments, the lower end of the first through hole is provided with an extension pipe body 520, the lower end opening of the extension pipe body 520 is provided with a second shutter 530 which can be opened and closed, and the second shutter 530 and the extension pipe body 520 are provided with a torsional spring 540 for driving the second shutter 530 to close, and when the three-dimensional controller 410 controls the ultramicroelectrode 430, the reference electrode 440 and the counter electrode 450 to be inserted into the holding cavity 230 at the scanning station, the second shutter 530 can be opened under the action of external force, wherein a sealing structure is arranged between the gas supply main pipe 620 and the hole wall of the second avoiding hole 211, and specifically, the sealing structure is a sealing plug sleeved on the gas supply main pipe 620. By arranging the second shutter 530 and the sealing structure between the gas supply main pipe 620 and the hole wall of the second avoiding hole 211, it is beneficial to prevent oxygen from entering the holding cavity 230 outside the scanning station. In addition, the tops of all the holding cavities 230 are communicated, so that when the nitrogen gas in the multi-cavity sample pool 200 is excessive, it can be discharged through the holding cavity 230 at the scanning station which is currently performing scanning test.

[0063] It should be noted that in some embodiments, the base electrode 210 is one of FTO conductive glass and ITO conductive glass.

[0064] The use method of the embodiment of the application is applied to the above-mentioned microorganism photoelectrochemical characterization device, and comprises the following steps:

[0065] A sterilized microorganism culture medium and a redox substance are selected to prepare an electrolyte solution and put into each holding cavity 230;

[0066] Different samples are respectively put into different holding cavities 230;

[0067] The nitrogen gas is continuously supplied into the holding cavity 230 by the gas supply device, which is beneficial to ensure that the samples in the holding cavity are in an anaerobic state;

[0068] The ultramicroelectrode 430, the reference electrode 440 and the counter electrode 450 are fixed on the three-dimensional controller 410, and the ultramicroelectrode 430, the reference electrode 440 and the counter electrode 450 are connected to the electrochemical workstation 420;

[0069] The light device is turned on to provide light for the accommodating cavity 230 at the scanning station;

[0070] The ultramicroelectrode 430, the reference electrode 440 and the counter electrode 450 are inserted into the accommodating cavity 230 at the scanning station by the three-dimensional controller 410, and the ultramicroelectrode 430 is driven to move to a preset position to complete the positioning of the ultramicroelectrode 430 and the drawing of the approaching curve;

[0071] The ultramicroelectrode 430, the reference electrode 440 and the counter electrode 450 are controlled by the three-dimensional controller 410 to scan the sample in the accommodating cavity 230 at the scanning station, and then the ultramicroelectrode 430, the reference electrode 440 and the counter electrode 450 are lifted by the three-dimensional controller 410 to above the multi-cavity sample pool 200, and then the multi-cavity sample pool 200 is driven to rotate by the driving device to make the next accommodating cavity 230 rotate to the scanning station and scan the sample in the accommodating cavity 230 according to the above steps, and the cycle is repeated until the scanning of the samples in all the accommodating cavities 230 is completed;

[0072] After a preset time interval, the samples in all the accommodating cavities 230 are scanned again according to the above steps.

[0073] In the description of the present specification, if the description of the terms such as "one embodiment", "some embodiments", "exemplary embodiment", "example", "specific example" and "some examples" is involved, it means that the specific features, structures, materials or characteristics described in connection with the embodiment or example are contained in at least one embodiment or example of the present application. In the present specification, the exemplary description of the above terms does not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described can be combined in any one or more embodiments or examples in a suitable manner.

[0074] Although the embodiments of the present application have been shown and described, those skilled in the art can understand that various changes, modifications, replacements and variations can be made to the embodiments without departing from the principles and purposes of the present application, and the scope of the present application is defined by the claims and their equivalents.

Claims

1. A device for photoelectrochemical characterization of microorganisms, characterized in that, include: A bracket (100) having a scanning station; A multi-cavity sample cell (200) is rotatably mounted on the support (100). The multi-cavity sample cell (200) includes a transparent base electrode (210) and a sidewall structure (220) disposed on the base electrode (210). The sidewall structure (220) and the base electrode (210) enclose a plurality of annularly distributed receiving cavities (230) for holding samples. A lighting device is mounted on the support (100) and is capable of providing illumination to the receiving cavity (230); A scanning electrochemical microscope apparatus includes a three-dimensional controller (410), an electrochemical workstation (420), an ultramicroelectrode (430), a reference electrode (440), and a counter electrode (450). The three-dimensional controller (410) is used to control the ultramicroelectrode (430), the reference electrode (440), and the counter electrode (450) to scan the sample located in the receiving cavity (230) at the scanning station. The electrochemical workstation (420) is used to connect the ultramicroelectrode (430), the reference electrode (440), and the counter electrode (450) and collect the electrochemical information generated by scanning the sample. A cover (500) is provided on the top of the multi-cavity sample cell (200). The cover (500) is provided with a first through hole for passing through the micro electrode (430), the reference electrode (440) and the counter electrode (450) for each of the receiving cavities (230). A gas supply device is mounted on the support (100) and is used to supply nitrogen gas into the receiving cavity (230); A driving device is mounted on the support (100) and is used to drive the multi-cavity sample cell (200) to rotate so that the different receiving cavities (230) can be rotated sequentially to the scanning station.

2. The device for photoelectrochemical characterization of microorganisms of claim 1, wherein, The illumination device includes a light source (310) and an on / off device (320). The on / off device (320) is disposed on the support (100) and located below the multi-cavity sample cell (200). The light source (310) is disposed on the support (100) and located below the on / off device (320). The light source (310) is used to provide illumination to the receiving cavity (230). The on / off device (320) can control the on / off of the optical path between the receiving cavity (230) located at the scanning station and the light source (310).

3. The device for photoelectrochemical characterization of microorganisms of claim 2, wherein, The on-off device (320) comprises a base (321) and a first baffle (322), the base (321) is fixedly arranged at the bottom of the base electrode (210), the base (321) is provided with a second through hole (3211) corresponding to each accommodating cavity (230) for the light path, and the side edge of the base (321) is provided with a insertion hole (3212) communicated with the second through hole (3211), and the first baffle (322) is movably arranged in the insertion hole (3212) and can block the light path passing through the second through hole (3211).

4. The device for photoelectrochemical characterization of microorganisms according to claim 3, characterized in that, The bracket (100) is provided with a supporting seat (800) corresponding to the base (321), the supporting seat (800) is provided with an annular groove (810), and the annular groove (810) is provided with a steel ball (820) for supporting the base (321).

5. The device for photoelectrochemical characterization of microorganisms of claim 3, wherein, The driving device comprises a motor (710), a rotating seat (720), a first bevel gear (730), a second bevel gear (740) and a control device, the motor (710) is arranged on the bracket (100) and is electrically connected with the control device, the upper end of the rotating seat (720) is fixedly connected with the base electrode (210) or the base (321), the first bevel gear (730) is fixedly arranged at the lower end of the rotating seat (720), the second bevel gear (740) is arranged at the output end of the motor (710) and is meshed with the first bevel gear (730), so that the motor (710) can drive the rotating seat (720) to rotate, thereby driving the multi-cavity sample pool (200) to rotate, and the control device is used for controlling the motor (710), so that different accommodating cavities (230) can be automatically rotated to the scanning station in sequence.

6. The device of claim 1, wherein the device is configured to perform the photoelectrochemical characterization of the microorganism by: The gas supply device comprises an air inlet pipeline (610), a gas supply main pipe (620), a gas supply branch pipe (630) and a rotary joint (640), one end of the air inlet pipeline (610) is used for being connected with a nitrogen gas source, the other end of the air inlet pipeline (610) is connected with the lower end of the gas supply main pipe (620) through the rotary joint (640), so that the gas supply main pipe (620) can rotate relative to the air inlet pipeline (610), and the upper end of the gas supply main pipe (620) is connected with a plurality of gas supply branch pipes (630) corresponding to the plurality of accommodating cavities (230) in one-to-one correspondence, and the plurality of gas supply branch pipes (630) are communicated with the plurality of accommodating cavities (230) in one-to-one correspondence.

7. The microbial photoelectrochemical characterization device as described in claim 1, characterized in that, The first through hole is provided with a flexible blocking piece (510), and the flexible blocking piece (510) is provided with a third through hole (511).

8. The device for photoelectrochemical characterization of microorganisms according to claim 7, characterized in that, The lower end of the first through hole is provided with an extension pipe body (520), and the lower end opening of the extension pipe body (520) is provided with a second shutter (530) which can be opened and closed in rotation, and a torsion spring (540) is arranged between the second shutter (530) and the extension pipe body (520) to drive the second shutter (530) to close, and the top portions of all the containing cavities (230) are communicated.

9. The device of claim 1, wherein the device is configured to perform the photoelectrochemical characterization of the microorganism by: The base electrode (210) is one of FTO conductive glass and ITO conductive glass. ​ 10. A use method applied to the microorganism photoelectrochemical characterization device according to any one of claims 1 to 9, comprising the following steps: An electrolyte solution is prepared by selecting a sterilized microorganism culture medium and a redox substance and is placed into each containing cavity (230); Different samples are respectively placed into different containing cavities (230); Nitrogen gas is continuously supplied into the containing cavities (230) through a gas supply device; The ultramicroelectrode (430), the reference electrode (440) and the counter electrode (450) are fixed on the three-dimensional controller (410), and the ultramicroelectrode (430), the reference electrode (440) and the counter electrode (450) are connected to the electrochemical workstation (420); The light illumination device is turned on to provide light illumination for the containing cavities (230) located at the scanning station; The ultramicroelectrode (430), the reference electrode (440) and the counter electrode (450) are inserted into the containing cavities (230) located at the scanning station through the three-dimensional controller (410), and the ultramicroelectrode (430) is driven to move to a preset position to complete positioning of the ultramicroelectrode (430) and drawing of an approach curve; The ultramicroelectrode (430), the reference electrode (440) and the counter electrode (450) are controlled to scan the samples in the containing cavities (230) located at the scanning station through the three-dimensional controller (410), and then the ultramicroelectrode (430), the reference electrode (440) and the counter electrode (450) are lifted above the multi-cavity sample pool (200) through the three-dimensional controller (410), and then the multi-cavity sample pool (200) is driven to rotate through the driving device, so that the next containing cavity (230) is rotated to the scanning station and the sample in the containing cavity (230) is scanned according to the above steps, and the scanning is repeated in this way until the scanning of the samples in all the containing cavities (230) is completed; After a preset time interval, the samples in all the containing cavities (230) are scanned again according to the above steps.

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