An expression vector for a biosynthetic metabolic pathway gene of 2-azetidinone and a method for synthesizing 2-azetidinone

By genetically modifying and optimizing the 2-azhexane cyclohexanone synthesis pathway of Corynebacterium glutamicum, and using the ChnR-Pb molecular probe system to initiate the expression of key genes, the problem of low yield of 2-azhexane cyclohexanone biosynthesis was solved, and efficient and low-cost production of 2-azhexane cyclohexanone was achieved.

CN116218895BActive Publication Date: 2026-04-24SHENZHEN INST OF ADVANCED TECH CHINESE ACAD OF SCI
View PDF 2 Cites 0 Cited by

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
SHENZHEN INST OF ADVANCED TECH CHINESE ACAD OF SCI
Filing Date
2021-12-03
Publication Date
2026-04-24

AI Technical Summary

Technical Problem

The existing technology for the biosynthesis of 2-azhexanecycloone has low yield and requires the addition of lysine as a raw material, resulting in high production costs. Enzyme activity also limits its synthesis.

Method used

By genetically modifying Corynebacterium glutamicum, a high-copy plasmid p208-HCP vector was constructed. The expression of key enzymes in the 2-azhexanecycloketone biosynthesis pathway was optimized. The expression of key genes in the 2-azhexanecycloketone biosynthesis pathway was initiated using the molecular probe system ChnR-Pb, reducing lysine dependence and improving enzyme catalytic efficiency.

Benefits of technology

A high-yield synthesis of 2-azhexanecycloone was achieved in Corynebacterium glutamicum, reducing the cost of lysine use, removing enzyme activity limitations, and greatly increasing biosynthetic yield.

✦ Generated by Eureka AI based on patent content.

Smart Images

  • Figure CN116218895B_ABST
    Figure CN116218895B_ABST
Patent Text Reader

Abstract

The application discloses an expression vector of a biosynthetic metabolic pathway gene of 2-azacyclohexanone and a synthesis method of 2-azacyclohexanone. The expression vector comprises a first promoter and, sequentially inserted downstream of the first promoter, davA-davB genes or davB-davA genes, a Pb promoter or a mutant thereof, an act gene or an orf26 gene or a caiC gene, a ChnR gene or a mutant thereof. The expression vector is further transferred into glutamic acid corynebacterium with high lysine yield, and is subjected to fermentation culture to efficiently synthesize 2-azacyclohexanone. The application synthesizes 2-azacyclohexanone in glutamic acid corynebacterium for the first time, and utilizes an optimized 2-azacyclohexanone molecular probe system to start the expression of key genes in the 2-azacyclohexanone biosynthetic metabolic pathway for the first time, so that high yield of 2-azacyclohexanone is realized. The synthesis method reduces the production cost required by using lysine as a synthesis raw material, eliminates a rate-limiting step, and greatly improves the biosynthesis yield of 2-azacyclohexanone.
Need to check novelty before this filing date? Find Prior Art

Citation Information

Patent Citations

  • Method for producing lactam

    CN107438667A

  • Method for preparing magnetic polymer microspheres and application thereof

    CN113304700A