Electrophoretic systems and electrophoretic analysis methods

Abstract

Provided is an electrophoresis system and an electrophoresis analysis method. The electrophoresis system includes an electrophoresis device including a measurement unit that measures a measurement target that has been separated by electrophoresis and a current detection unit that detects a current flowing through a flow path. The electrophoresis system is configured to cause a display unit to display, during measurement of the measurement target, each of a time-series of values of a measurement value of the measurement target measured by the measurement unit and a time-series of values of a current detection value of the current detected by the current detection unit.

Description

Technical Field

[0001] This invention relates to an electrophoresis system and an electrophoresis analysis method. Background Technology

[0002] Previously, an electrophoresis system for performing electrophoresis was known. Such a system is disclosed, for example, in International Publication No. 2018 / 181432.

[0003] The electrophoresis system described in International Publication No. 2018 / 181432 includes an electrophoresis apparatus and an electrophoresis analysis apparatus. In the electrophoresis apparatus of this system, a direct current voltage is applied to electrodes inserted into electrode slots located at both ends of a capillary tube, which serves as the flow path for the sample to be measured during electrophoresis. Then, when electrophoresis begins by applying a direct current voltage to the electrodes, the sample moves due to electrophoresis. The capillary tube is then monitored through a detection window, and actual waveform data representing the time-varying brightness of the fluorescence emitted from the moving sample is generated and output to the electrophoresis analysis apparatus.

[0004] Although not described in the aforementioned International Publication No. 2018 / 181432, in cases where anomalies occur during electrophoresis-based measurements, insufficient measurement precision leads to abnormal results. Therefore, operators performing the measurements need to individually identify the factors considered to be causing the anomalies in the electrophoresis-based measurement results (measured values). Thus, the task of identifying the factors causing the anomalies becomes a burden for the operator. Summary of the Invention

[0005] The present invention was made to solve the problems described above. One object of the present invention is to provide an electrophoresis system and electrophoresis analysis method that can reduce the workload of operators in identifying abnormal factors when abnormalities occur in the measured values ​​obtained by electrophoresis.

[0006] To achieve the above objectives, the electrophoresis system of the first aspect of the present invention includes an electrophoresis apparatus comprising: a measuring unit that measures a test object separated by electrophoresis in a flow path including a separation flow path for separating the test object; and a current detection unit that detects the current flowing through the flow path. The electrophoresis system is configured such that, during the measurement of the test object, a display unit displays the time series values ​​of the measurement values ​​of the test object measured by the measuring unit and the time series values ​​of the current detection values ​​of the current detected by the current detection unit.

[0007] The electrophoretic analysis method in the second aspect of the present invention includes the following steps: obtaining a measurement value, which is obtained by measuring a test object that has been separated by electrophoresis in a flow path including a separation flow path for separating the test object; obtaining a current detection value of the current flowing through the flow path; and, during the measurement of the test object, displaying the time series values ​​of the measurement value and the time series values ​​of the current detection value on a display unit.

[0008] In the electrophoresis system of the first aspect and the electrophoretic analysis method of the second aspect described above, during the measurement of the test object, the display unit displays the time series values ​​of the measured values ​​and the time series values ​​of the current detection values. Here, if both the measured value and the current are abnormal, the possibility of abnormalities such as the concentration of the test object itself, abnormalities in the power supply for the flowing current, or abnormalities in the process of filling the flow path with the test object is considered. On the other hand, if the measured value is abnormal but the current is not abnormal, the possibility of deterioration of the components forming the flow path is considered. Therefore, as described above, by displaying the time series values ​​of the measured values ​​and the time series values ​​of the current detection values ​​during the measurement of the test object, it is possible to confirm whether the measured value and the current are abnormal by visually recognizing the display unit. Therefore, it is possible to confirm whether the current is abnormal when the measured value is abnormal, and thus it is possible to pinpoint the factors causing the abnormality of the measured value from the following possibilities: abnormalities such as the concentration of the test object itself, abnormalities in the power supply for the flowing current, or abnormalities in the process of filling the flow path with the test object; and the possibility of deterioration of the components forming the flow path. As a result, it can reduce the workload of operators in identifying abnormal factors when the measured values ​​obtained by electrophoresis are abnormal. Attached Figure Description

[0009] Figure 1 This is a block diagram showing the overall structure of the electrophoresis system of this embodiment.

[0010] Figure 2 This is a schematic diagram illustrating the structure of the electrophoresis apparatus of this embodiment.

[0011] Figure 3 This is a diagram illustrating the structure of a chip with flow paths for electrophoresis.

[0012] Figure 4 This is a diagram showing an example of a measurement value obtained through the measurement unit.

[0013] Figure 5 This is a diagram showing an example of the current detection value of the current flowing through the flow path.

[0014] Figure 6 This is a diagram showing an example of the voltage detection value of the voltage applied to the flow path.

[0015] Figure 7 This is a diagram showing an example of the display unit during a measurement process.

[0016] Figure 8 This diagram illustrates the switching of the display on the display unit.

[0017] Figure 9 This is a flowchart illustrating an electrophoretic analysis method of one implementation. Detailed Implementation

[0018] The following describes one embodiment of the invention based on the accompanying drawings.

[0019] (Overall structure of the electrophoresis system)

[0020] Reference Figures 1 to 8 An electrophoresis system 100 according to one embodiment of the present invention will be described.

[0021] like Figure 1 As shown, the electrophoresis system 100 of this embodiment includes an electrophoresis apparatus 101 and an analysis apparatus 102.

[0022] The electrophoresis apparatus 101 separates the analyte by electrophoresis using three chips 60a, 60b, and 60c, thereby measuring (analyzing) the components contained in the analyte. Specifically, in the electrophoresis apparatus 101, a sample preparation section 71 (see reference 60a, 60b, and 60c) is pre-positioned on the plate 70 and the sample preparation section 71. Figure 2 The measurement targets are located in flow paths 61 (refer to) respectively set in chips 60a to 60b. Figure 3 The particles are separated by electrophoresis. Then, the electrophoresis apparatus 101 measures the separation degree (mobility) of the particles separated by electrophoresis. Furthermore, chips 60a to 60c are examples of the "flow path components" of this disclosure.

[0023] <Structure of the electrophoresis apparatus>

[0024] like Figure 1 and Figure 2 As shown, the electrophoresis apparatus 101 includes a supply unit 10, a voltage application unit 20, a measurement unit 30, and a control unit 40.

[0025] In the electrophoresis apparatus 101, the analyte and separation buffer are supplied to the flow paths 61 of chips 60a, 60b and 60c by the operation of the supply unit 10 for electrophoresis-based determination.

[0026] The analytes may include, for example, DNA (Deoxyribonucleic acid), RNA (ribonucleic acid), or proteins. The analytes include the sample for which the separation (migration) of each component obtained by electrophoresis is to be measured, and a reference sample serving as a benchmark for electrophoresis-based measurements of the sample. The sample is measured using the measurement unit 30 described later, and the measured value 111 (refer to...) is... Figure 4 This refers to analytes whose electrophoretic separation (measurement waveform) is unknown. In contrast, reference samples are analytes containing nucleic acids or proteins whose separation characteristics, such as molecular weight (chain length), are already well-defined.

[0027] Furthermore, the test objects are arranged on the plate 70 and the sample arrangement section 71. The plate 70 has multiple wells 70a serving as multiple arrangement positions for the test objects. For example, the plate 70 has 96 wells 70a in a 8×12 configuration. The plate 70 is arranged by the operator in the plate arrangement position inside the electrophoresis apparatus 101 with all or part of each of the multiple wells 70a containing multiple types of test objects. Then, the test objects are arranged independently of the plate 70 in the sample arrangement section 71. The sample arrangement section 71 has multiple arrangement positions for the test objects, and these multiple arrangement positions are designated as wells 71a. For example, if the measured value 111 is unknown, the test object to be analyzed is arranged in the well 70 of the plate 70, and a reference sample with a known measured value 111 is arranged independently of the plate 70 in the well 71a of the sample arrangement section 71.

[0028] The separation buffer is filled into the flow path 61 of chips 60a, 60b and 60c respectively (see reference) before being supplied to the analyte. Figure 3 The separation medium includes, for example, a pH buffer material and at least one of a water-soluble polymer (such as a cellulose-based polymer). The separation buffer is filled into a buffer container (not shown). Alternatively, the separation buffer may be placed in the plate 70 or the sample preparation section 71. In the electrophoresis apparatus 101, the analyte is supplied with the separation buffer pre-filled inside the flow path 61 for electrophoresis.

[0029] like Figure 2 As shown, the supply unit 10 includes a probe 11 and a pump 12. The supply unit 10 supplies the separation buffer and the test objects (samples and reference samples) disposed on the plate 70 or the sample placement unit 71 to the chips 60a to 60c by moving the probe 11. The pump 12 adjusts the pressure of the probe 11 for drawing and ejecting the separation buffer and the test objects.

[0030] like Figure 3As shown, each of chips 60a to 60c has a flow path 61 inside. Chips 60a, 60b, and 60c have identical structures. In the following description, details of chip 60a are illustrated and explained, while descriptions of chips 60b and 60c are omitted as they are identical to those of chip 60a.

[0031] Chip 60a is an electrophoresis microchip with a flow path 61 for electrophoresis disposed inside a pair of combined flat plate-shaped components. Flow path 61 includes a separation flow path 62 and a preparation flow path 63. The separation flow path 62 and the preparation flow path 63 are arranged to intersect each other. The separation flow path 62 is provided for separating the analyte by electrophoresis. In addition, the preparation flow path 63 is provided for guiding the analyte to the separation flow path 62.

[0032] Furthermore, reservoirs 64a and 64b are provided at both ends of the preparation flow path 63, serving as spaces for supplying and aspirating the separation buffer and the analyte. Similarly, reservoirs 64c and 64d are provided at both ends of the separation flow path 62. Moreover, multiple electrodes 65a, 65b, 65c, and 65d are arranged in the flow path 61. Specifically, electrodes 65a and 65b are respectively arranged in the reservoirs 64a and 64b at both ends of the preparation flow path 63. Moreover, electrodes 65c and 65d are respectively arranged in the reservoirs 64c and 64d at both ends of the separation flow path 62.

[0033] In the electrophoresis apparatus 101, electrophoresis is performed by applying voltage from the voltage application unit 20 to a plurality of electrodes 65a to 65d disposed in the flow path 61. Furthermore, the magnitude of the voltage applied to the electrodes 65a to 65d is controlled by the control unit 40. In addition, three voltage application units 20 are provided, each corresponding to a chip 60a to 60c (see reference). Figure 2 The voltage application unit 20 applies a DC voltage to the flow path 61 of each of the chips 60a to 60c. That is, for chips 60b and 60c, the DC voltage is also applied to the flow path 61 by the voltage application unit 20 in the same way as for chip 60a.

[0034] In the electrophoresis apparatus 101, when performing electrophoresis-based measurements on the chip 60a, firstly, the supply unit 10 fills the entire interior of the flow path 61 (separation flow path 62 and preparation flow path 63) with separation buffer. Then, for example, the supply unit 10 supplies the sample material drawn from the designated well 70a of the plate 70 to the reservoir 64a of the preparation flow path 63. Next, by applying a predetermined voltage to the electrodes 65a to 65d using the voltage application unit 20, the sample material moves within the preparation flow path 63 until it intersects with the separation flow path 62. Then, by varying the voltage applied from the voltage application unit 20 to each of the electrodes 65a to 65d, the sample material moves towards the electrode 65d (reservoir 64d) within the separation flow path 62 while being separated by electrophoresis.

[0035] At this time, in the electrophoretic separation measurement, the analyte moves within the separation flow path 62 at different speeds depending on the molecular weight (chain length) and other separation characteristics of its constituent components. In the electrophoresis apparatus 101, the separation characteristics of each component of the analyte are measured by sequentially measuring the components arriving at the measurement positions 66 within the separation flow path 62. Thus, in the electrophoresis apparatus 101, the components contained in the analyte are measured according to the degree of separation (migratory power).

[0036] like Figure 2 As shown, in this embodiment, the measurement unit 30 measures the test objects that have been separated by electrophoresis in the flow paths 61 of each of the plurality of (3) chips 60a to 60c. For example, the measurement unit 30 performs fluorescence detection on the components of the test objects separated by electrophoresis. The measurement unit 30 has a measurement position 66 (see reference) in the separation flow path 62. Figure 3 An LED 31 (light-emitting diode) is used to illuminate the sample. Furthermore, the excitation light from the LED 31 is irradiated onto each component of the sample as it moves through the separation flow path 62 while being separated by electrophoresis, thereby exciting each component of the sample to emit fluorescence. The measurement unit 30 measures this fluorescence using a photomultiplier tube 32, for example, via an optical fiber and filter components, thereby measuring the electrophoretic separation degree of the sample.

[0037] like Figure 4 As shown, the photomultiplier tube 32 outputs a measurement signal representing the measurement value 111 to the control unit 40 based on the detected fluorescence intensity. In the measurement value 111 measured by the measurement unit 30, the measurement object, which moves while being separated by electrophoresis, passes the measurement position 66 (see reference). Figure 3The time of the peak indicates the largest value (peak). Therefore, based on the size and position (time) of the peaks of each component contained in the measured object, the composition and quantity of each component contained in the measured object can be analyzed.

[0038] Furthermore, the electrophoresis apparatus 101 is equipped with a cleaning mechanism (not shown). The electrophoresis apparatus 101 is configured such that whenever a measurement of a analyte is performed in each part, including chips 60a-60c and the supply unit 10, the cleaning mechanism cleans the residual analyte and separation buffer in the flow path 61, thereby repeating the measurement of each chip in chips 60a-60c multiple times.

[0039] In addition, such as Figure 2 and Figure 3 As shown, the electrophoresis apparatus 101 includes a current detection unit 21 and a voltage detection unit 22. The current detection unit 21 and the voltage detection unit 22 respectively detect the current and voltage output from each of the plurality of (3) voltage application units 20. The current detection unit 21 detects the current flowing through the current path 61 of each of the chips 60a to 60c due to the voltage applied by the voltage application unit 20. The voltage detection unit 22 detects the voltage applied by the voltage application unit 20 to the current path 61 of each of the chips 60a to 60c.

[0040] For example, in chip 60a, the current detection unit 21 detects the current flowing through the current path 61 by detecting the current flowing through electrodes 65a, 65b, 65c, and 65d respectively. Similarly, the voltage detection unit 22 detects the voltage applied to the current path 61 by detecting the voltage applied to electrodes 65a, 65b, 65c, and 65d respectively. Then, the current detection unit 21 displays the detected current detection value 112 (see reference 112). Figure 5 The detection signal is output to the control unit 40, and the voltage detection unit 22 outputs the detected voltage value 113 (refer to...) Figure 6 The detection signal is output to the control unit 40. The same applies to chips 60b and 60c.

[0041] Therefore, such as Figure 5 and Figure 6 As shown, the control unit 40 is configured to acquire four current detection values ​​112 and four voltage detection values ​​113 for each chip 60a (60b or 60c) in a manner corresponding to the four electrodes 65a to 65d. Furthermore, in Figure 5 and Figure 6 At time point T1, the current detection value 112 and the voltage detection value 113 change significantly. This means that at time point T1, the period of moving the object being measured in the preparation flow path 63 is switched to the period of moving the object being measured in the separation flow path 62.

[0042] The control unit 40 controls the operation of each part of the electrophoresis apparatus 101. The control unit 40 is, for example, a microcomputer (microcontroller) equipped with a processing unit such as a CPU (Central Processing Unit) and a storage device such as flash memory. Furthermore, the control unit 40 includes a communication module, enabling it to communicate with the analysis device 102. Moreover, based on drive signals from the analysis device 102, the control unit 40 controls the operation of each part of the electrophoresis apparatus 101 in a manner that sequentially performs electrophoretic measurements of the test objects arranged on the plate 70 and the sample placement unit 71.

[0043] Specifically, the control unit 40 operates the supply unit 10 based on a drive signal from the analysis device 102, thereby sequentially supplying the test objects arranged in the sample wells 70a of the plate 70 in a manner that, for example, one type of measurement is performed on each of the chips 60a to 60c. Then, the control unit 40 separates (moves) the test objects by applying voltage to the flow path 61 of each of the chips 60a to 60c using the voltage application unit 20. In addition, the control unit 40 acquires the measurement values ​​111 (electrophoresis maps) measured by the measurement unit 30, which is arranged in a manner corresponding to each of the chips 60a to 60c. Then, the control unit 40 acquires the measurement values ​​111 for each of the multiple sample wells 70a of the plate 70 and each of the sample wells 71a of the sample placement unit 71. Furthermore, the control unit 40 is configured to acquire the current detection value 112 detected by the current detection unit 21 and the voltage detection value 113 detected by the voltage detection unit 22 synchronously with the measurement performed by the measurement unit 30.

[0044] Then, for each of the chips 60a to 60c, the control unit 40 outputs in real time to the analysis device 102 the measured value 111 of the measured object measured by the measurement unit 30, the current detection value 112 of the current detected by the current detection unit 21, and the voltage detection value 113 of the voltage detected by the voltage detection unit 22 for each chip.

[0045] <Structure of the Analytical Device>

[0046] like Figure 1 As shown, the analysis device 102 includes an operation unit 51, a display unit 52, a storage unit 53, and a control unit 54. The analysis device 102 is a computer used to analyze the measurement value 111 obtained by the electrophoresis apparatus 101. The analysis device 102 is configured to communicate with the electrophoresis apparatus 101 and to acquire the measurement value 111, current detection value 112, and voltage detection value 113 obtained by the electrophoresis apparatus 101.

[0047] The operation unit 51 accepts input operations from the operator. Furthermore, the operation unit 51 outputs an operation signal based on the accepted input operation to the control unit 54. The operation unit 51 may be a pointing device such as a keyboard or mouse.

[0048] The display unit 52 is, for example, a monitor such as an LCD screen. Furthermore, the display unit 52 displays the input information according to the control of the control unit 54.

[0049] The storage unit 53 is composed of a storage device such as a hard disk drive or an SSD (Solid State Drive). The storage unit 53 stores the measured values ​​111, current detection values ​​112, and voltage detection values ​​113 obtained using the electrophoresis apparatus 101. Additionally, the storage unit 53 stores an electrophoresis analysis program 53a for operating the control unit 54. Furthermore, the storage unit 53 stores various parameters, including preset settings or settings (measurement conditions) input by the operator.

[0050] The control unit 54 is a computer including a CPU, RAM (Random Access Memory), and ROM (Read Only Memory). The control unit 54 executes the program (electrophoretic analysis program 53a) stored in the storage unit 53 to control each part of the analysis device 102. Furthermore, the control unit 54 is configured to communicate with the control unit 40 of the electrophoresis apparatus 101 via a communication module (not shown).

[0051] (Details of the control measures performed by the analysis device)

[0052] The control unit 54 sends an operation signal to the control unit 40 to activate the electrophoresis apparatus 101. Specifically, based on the input operation received by the operation unit 51, the control unit 54 acquires various parameters for performing electrophoresis. For example, based on the input operation to the operation unit 51, the control unit 54 acquires sample well information 52b (see reference) indicating that sample wells 70a and 71a, which are configured with the test objects to be measured, are located thereon. Figure 8 ), including measurement condition information such as the magnitude and duration of the applied voltage, and arrangement table information 52c indicating the measurement order of the measurement objects arranged in multiple sample wells 70a and 71a (refer to Figure 8Furthermore, the sample well information 52b, measurement condition information, and arrangement table information 52c can also be selected from the database pre-stored in the storage unit 53. Then, the control unit 54 sends a drive signal containing the acquired sample well information 52b, measurement condition information, and arrangement table information 52c to the control unit 40 of the electrophoresis apparatus 101. Then, the control unit 54 acquires in real time the measured values ​​111, current detection values ​​112, and voltage detection values ​​113 obtained by the control unit 40 based on the sent drive signal in response to the progress of the measurement.

[0053] Moreover, in this embodiment, such as Figure 7 As shown, the control unit 54 is configured to display the measured value 111, the current detection value 112, and the voltage detection value 113 on the display unit 52. Specifically, the control unit 54 is configured to display, during the measurement of the measured object, the time sequence values ​​of the measured value 111, the time sequence values ​​of four current detection values ​​112 corresponding to the current flowing through each of the plurality of electrodes 65a to 65d, and the time sequence values ​​of four voltage detection values ​​113 corresponding to the voltage applied to each of the plurality of electrodes 65a to 65d.

[0054] In detail, during the measurement of the object being measured, the control unit 54 displays the time series values ​​of the measured value 111, the current detection value 112, and the voltage detection value 113 on the display unit 52 while updating them in real time. Furthermore, the control unit 54 is configured to display the time series values ​​of the measured value 111, the current detection value 112, and the voltage detection value 113 not only during the measurement of the object being measured (while the object is moving to the separation flow path 62 and being measured by the measurement unit 30), but also while the object is being introduced into the preparation flow path 63. Additionally, the control unit 54 is configured to display the time series values ​​of the measured value 111, the current detection value 112, and the voltage detection value 113 acquired throughout the entire common measurement period as waveforms on the display unit 52.

[0055] That is, the control unit 54 is configured to display the time sequence values ​​of the measured value 111, the current detection value 112, and the voltage detection value 113 from the point in time after the measured object is supplied to the reservoir 64a in each chip 60a (60b or 60c) and the DC voltage is applied by the voltage application unit 20 until the present.

[0056] Furthermore, the control unit 54 is configured to display on the display unit 52, using different colors, the time series values ​​(waveforms) of four current detection values ​​112 corresponding to the current flowing through each of the plurality of electrodes 65a-65d, and the time series values ​​(waveforms) of four voltage detection values ​​113 corresponding to the voltage applied to each of the plurality of electrodes 65a-65d, respectively, in a recognizable manner. For example, the control unit 54 uses a red waveform to represent the time series values ​​of the current detection value 112 and voltage detection value 113 corresponding to electrode 65a. The control unit 54 uses a yellow waveform to represent the time series values ​​of the current detection value 112 and voltage detection value 113 corresponding to electrode 65b. The control unit 54 uses a blue waveform to represent the time series values ​​of the current detection value 112 and voltage detection value 113 corresponding to electrode 65c. The control unit 54 uses a green waveform to represent the time series values ​​of the current detection value 112 and voltage detection value 113 corresponding to electrode 65d. Furthermore, in Figure 7 (and Figure 8 In the diagram, solid lines, dashed lines, dotted lines, and double-dotted lines are used to represent the red, yellow, blue, and green waveforms, respectively.

[0057] Furthermore, the control unit 54 displays the waveforms representing the time series values ​​of the measured value 111, the time series values ​​of the voltage detection value 113, and the time series values ​​of the current detection value 112 sequentially side-by-side in the horizontal direction on the display unit 52. Additionally, the control unit 54 is configured to display the time series values ​​(waveforms) of the measured value 111, the current detection value 112, and the voltage detection value 113 on the display unit 52 according to each of the three chips 60a, 60b, and 60c.

[0058] Specifically, the control unit 54 assigns chip 60a the number 1, chip 60b the number 2, and chip 60c the number 3, arranging chips 60a, 60b, and 60c sequentially vertically. The display unit 52 then displays the time series values ​​(waveforms) of the measured values ​​111, current detection values ​​112, and voltage detection values ​​113 of each chip. That is, the control unit 54 displays the measured values ​​111, current detection values ​​112, and voltage detection values ​​113 obtained by the three chips 60a to 60c during the current analysis process in real time. Furthermore, the control unit 54 instructs the display unit 52 to display the position of the sample well 70a or 71a corresponding to the measurement object currently being measured among the three chips 60a to 60c.

[0059] For example, in Figure 7In the example shown, chip pairs from chip 60a (number 1), chip 60b (number 2), and chip 60c (number 3) are arranged at position X1A (see reference) in the sample arrangement section 71. Figure 8 The time series values ​​(waveforms) of the measured value 111, current detection value 112, and voltage detection value 113 obtained during the analysis (measurement) of the measured object in sample well 71a.

[0060] In addition, such as Figure 8 As shown, the control unit 54 is configured such that the display unit 52 displays, in addition to displaying the time series values ​​of the measured value 111, the current detection value 112, and the voltage detection value 113, the sample hole information 52b and the arrangement table information 52c. Furthermore, the time series values ​​of the measured value 111, the current detection value 112, and the voltage detection value 113 are displayed in such a manner that the time point at which the voltage application unit 20 begins applying voltage is set to 0 seconds, and the elapsed time up to the present is used as the horizontal axis.

[0061] Moreover, in this embodiment, such as Figure 7 and Figure 8 As shown, the control unit 54 is configured to switch between displaying the time series values ​​of the measured value 111, the current detection value 112, and the voltage detection value 113 on the display unit 52, and displaying, in addition to displaying the time series values ​​of the measured value 111, the current detection value 112, and the voltage detection value 113 on the display unit 52, and displaying the sample hole information 52b and the arrangement table information 52c on the display unit 52, based on a switching operation received by the operation unit 51. Specifically, the control unit 54 is configured to switch the display of the display unit 52 when a switching operation for switching the display is received based on a click action on the display switching button 52a displayed on the upper part of the display unit 52. That is, the control unit 54 is configured to switch the display of the display unit 52 based on the switching operation received by the operation unit 51. Figure 7 The state shown is the same as Figure 8 Switch between the shown states.

[0062] The sample hole information 52b is displayed on the upper left side of the display unit 52. The sample hole information 52b indicates which of the plurality of sample holes 70a in the plate 70 and which of the plurality of sample holes 71a in the sample placement unit 71 are configured with a test object. Specifically, the control unit 54 causes the display unit 52 to display the sample hole information 52b, which includes information indicating which sample hole 70a or 71a in the plate 70 and the sample hole 71a in the sample placement unit 71 is configured with a test object. In other words, the control unit 54 displays the information indicating which sample holes 70a and 71a are to be analyzed (measured) as the sample hole information 52b. In the sample hole information 52b, the sample holes 70a and 71a to be measured are displayed using a different color (e.g., blue) than the sample holes 70a and 71a that are not being measured. For example, the measurement of which test object is configured in which sample hole 70a and 71a is set based on input operations performed by the operator.

[0063] The arrangement information 52c is displayed on the upper right side of the display unit 52. The arrangement information 52c indicates the order in which electrophoresis-based measurements are performed on the test objects arranged in the multiple sample wells 70a and 71a using the chips 60a to 60c. The control unit 54 displays the time series values ​​(waveforms) of the test objects currently being measured in the chips 60a to 60c, including the measured value 111, the current detection value 112, and the voltage detection value 113, on the lower side of the display unit 52, and arranges the predetermined test objects to be measured in the chips 60a to 60c thereafter, together with the information indicating the sample wells 70a and 71a where the test objects are arranged, in the order of measurement from top to bottom.

[0064] In addition to showing the measurement sequence in the arrangement table information 52c, the control unit 54 also indicates, using chip numbers (1-3), which chip among chips 60a-60c will be used to measure the sample objects disposed in the sample wells 70a and 71a. Furthermore, the control unit 54 displays information in the arrangement table information 52c indicating the type of the sample object disposed in the corresponding sample wells 70a and 71a. As described above, the sample objects include two categories: reference samples that serve as a benchmark and samples whose resolution (waveform of the measured value 111) is unknown. In the arrangement table information 52c, the control unit 54 causes the display unit 52 to display the reference sample as a "sample standard" and the sample as a "sample," enabling identification of the type of sample object.

[0065] (Regarding electrophoretic analysis methods)

[0066] Next, refer to Figure 9The electrophoretic analysis method using the electrophoresis system 100 in this embodiment will be explained. Furthermore, the control processing in steps 201 to 208 is performed by the control unit 54 (analysis device 102) executing the electrophoretic analysis program 53a stored in the storage unit 53.

[0067] First, in step 201, measurement condition information for performing the measurement is acquired. Specifically, sample hole information 52b indicating the sample holes 70a and 71a on which the measurement objects to be measured are arranged, arrangement table information 52c indicating the measurement order, and information indicating the type of measurement objects arranged in the sample holes 70a and 71a, along with measurement condition information including information indicating the magnitude and duration of the voltage applied by the voltage application unit 20, are acquired (set). Furthermore, this information can be acquired based on input operations to the operation unit 51, or it can be acquired from information pre-stored in the storage unit 53, etc.

[0068] Next, in step 202, a drive signal containing the acquired measurement condition information is sent to the control unit 40 of the electrophoresis apparatus 101. In addition to the measurement condition information, the drive signal also includes the acquired sample well information 52b and arrangement table information 52c. Based on this drive signal, in the electrophoresis apparatus 101, electrophoretic analysis (measurement) using chips 60a to 60c is performed on each measurement object in the specified sample wells 70a and 71a in a predetermined order.

[0069] Next, in step 203, a measurement value 111 is acquired based on the measurement by the measurement unit 30 of the electrophoresis apparatus 101. Measurement values ​​111 are acquired sequentially in real time as the measurement progresses. Specifically, the test object disposed in one of the sample wells 70a and 71a corresponding to a pre-set measurement sequence is supplied to one of the chips 60a to 60c, and a voltage is applied using the voltage application unit 20. Thus, from the point in time when the test object is introduced into the preparation flow path 63 from one of the chips 60a to 60c, the measurement value 111 obtained by the measurement unit 30 is acquired in real time.

[0070] In step 204, a current detection value 112 is acquired to measure the current flowing through the flow path 61 due to the voltage applied by the voltage application unit 20 of the electrophoresis apparatus 101. Specifically, four current detection values ​​112 are acquired in real time, synchronous with the measured value 111, to measure the current flowing through each of the electrodes 65a to 65d.

[0071] In step 205, a voltage detection value 113 is acquired, which is the voltage applied to the flow path 61 by the voltage application unit 20 of the electrophoresis apparatus 101. Specifically, four voltage detection values ​​113 are acquired in real time, synchronous with the measured value 111, of the voltage applied to each of the electrodes 65a to 65d, through the detection performed by the voltage detection unit 22. Furthermore, the acquisition of the measured value 111, the current detection value 112, and the voltage detection value 113 in steps 203 to 205 are performed at approximately the same time.

[0072] Next, in step 206, the time series values ​​(waveforms) of the acquired measured value 111, current detection value 112, and voltage detection value 113 are displayed on the display unit 52. The time series values ​​of the measured value 111, current detection value 112, and voltage detection value 113 are displayed side by side as waveforms over the entire common measurement period from the time point when the voltage is applied from the voltage application unit 20 to the present. Furthermore, the acquisition and display of the measured value 111, current detection value 112, and voltage detection value 113 in steps 203 to 206 are updated in real time during the process of introducing the object to be measured into the preparation flow path 63 and during the measurement of the object.

[0073] Next, in step 207, it is determined whether a switching operation for changing the display has been accepted. If the switching operation has been accepted, the process proceeds to step 208. Otherwise, if the switching operation has not been accepted, the control process ends.

[0074] In step 208, based on the received switching operation, a switch is made between displaying the time series values ​​of the measured value 111, the current detection value 112, and the voltage detection value 113 on the display unit 52, and displaying the sample hole information 52b and the arrangement table information 52c in addition to displaying the time series values ​​of the measured value 111, the current detection value 112, and the voltage detection value 113.

[0075] The control processing in steps 203 to 208 continues until the measurement (analysis) of the test object located in the designated sample well 70a or 71a is completed in one chip 60a (60b or 60c). Then, after the measurement (analysis) of the test object located in the designated sample well 70a or 71a has been completed, the measurement of the new test object located in the next sample well 70a or 71a is performed according to the arrangement table information 52c. In addition, the measurements in each chip of chips 60a to 60c are performed simultaneously.

[0076] (Effects of this implementation method)

[0077] In this embodiment, the following effects can be obtained.

[0078] In the electrophoresis system 100 of this embodiment, as described above, during the measurement of the test object, the display unit 52 displays the time series values ​​of the measured value 111 and the time series values ​​of the current detection value 112. Here, if both the measured value 111 and the current (current detection value 112) are abnormal, possibilities such as abnormalities in the concentration of the test object itself, abnormalities in the power supply section (voltage application unit 20, wiring components, or electrodes 65a-65d, etc.) used for the flowing current, or abnormalities in the process of filling the flow path 61 (abnormalities in the supply unit 10) are considered. On the other hand, if the measured value 111 is abnormal but the current (current detection value 112) is not abnormal, the possibility of deterioration in the components (chips 60a-60c) on which the flow path 61 is formed is considered. Therefore, as described above, during the measurement of the object being measured, the display unit 52 displays the time series values ​​of the measured value 111 and the time series values ​​of the current detection value 112. This allows for visual identification of the display unit 52 to confirm whether either the measured value 111 or the current (current detection value 112) has become abnormal. Thus, it is possible to confirm whether the current has become abnormal when the measured value 111 becomes abnormal, and thus, factors contributing to the abnormality of the measured value 111 can be identified from the following: abnormalities in the concentration of the object being measured, abnormalities in the power supply components used for the flowing current (voltage application unit 20, wiring components, or electrodes 65a-65d, etc.), or abnormalities in the process of filling the flow path 61 with the object being measured (abnormalities in the supply unit 10); and the possibility of deterioration in the components forming the flow path 61 (chips 60a-60c). As a result, the workload of the operator in identifying the factors contributing to the abnormality when the measured value 111 obtained by electrophoresis becomes abnormal can be reduced.

[0079] Furthermore, in the above embodiments, further effects can be obtained by configuring it as follows.

[0080] That is, in this embodiment, as described above, the electrophoresis system 100 includes an analysis device 102 for acquiring measured values ​​111 and current detection values ​​112. The analysis device 102 (control unit 54) is configured to display the time series values ​​of the measured values ​​111 and current detection values ​​112 on a display unit 52 while updating them in real time during the measurement of the object being measured. With this configuration, by using the analysis device 102 to display the time series values ​​of the measured values ​​111 and current detection values ​​112 on the display unit 52 while updating them in real time, the operator can visually recognize the latest time changes of the measured values ​​111 and current detection values ​​112 by visually observing the display unit 52. Therefore, the operator can more easily determine whether the measured values ​​111 and current detection values ​​112 are abnormal. Consequently, it is easier to determine whether the current is abnormal, thus further reducing the operator's workload in identifying factors causing abnormalities in the measured values ​​111.

[0081] Furthermore, in this embodiment, as described above, the electrophoresis apparatus 101 includes a voltage detection unit 22 that detects the voltage applied to the flow path 61. The analysis device 102 (control unit 54) is configured such that, in addition to displaying the time series values ​​of the measured value 111 and the current detection value 112 on the display unit 52, it also displays the time series value of the voltage detection value 113 detected by the voltage detection unit 22 during the measurement of the object being measured. With this configuration, the operator can confirm the time series value of the voltage detection value 113 in addition to the time series values ​​of the measured value 111 and the current detection value 112 during the measurement process. Here, if an abnormality occurs in the voltage, it is considered that the abnormality may not be due to the object being measured itself or the components forming the flow path 61 (chips 60a-60c), but rather to a problem with the structure used for flowing current (voltage application unit 20, wiring components, or electrodes 65a-65d, etc.). Therefore, in addition to confirming the measured value 111 and the current detection value 112, the operator also confirms the voltage detection value 113, thereby further identifying the type of cause of the abnormality in the measured value 111. As a result, the operator's workload in identifying the factors causing the abnormality when the measured value 111 obtained by electrophoresis is abnormal can be further reduced.

[0082] Furthermore, in this embodiment, as described above, the analysis device 102 (control unit 54) is configured to display the time series values ​​of the measured value 111 and the current detection value 112, respectively, as waveforms on the display unit 52 throughout the entire common measurement period. With this configuration, it is easy to compare the time when the measured value 111 becomes abnormal with the time when the current detection value 112 becomes abnormal. Therefore, the operator can more intuitively confirm whether the current detection value 112 is abnormal when the measured value 111 becomes abnormal. As a result, the operator's workload in identifying abnormal factors when the measured value 111 becomes abnormal can be further reduced.

[0083] Furthermore, in this embodiment, as described above, the analysis device 102 (control unit 54) is configured to display the time series values ​​of the measured value 111 and the current detection value 112 side by side on the display unit 52. Here, the electrophoretic measured value 111 changes in a manner that increases whenever each component of the separated test object is detected, thus becoming a waveform with multiple peaks over time. On the other hand, the current detected in electrophoresis is a roughly constant direct current, so the time series values ​​of the current detection value 112 represent roughly constant values. Therefore, it is considered that if the measured value 111 and the current detection value 112, which have different shapes, are displayed superimposed, the visibility of both the measured value 111 and the current detection value 112 will decrease. In contrast, in this embodiment, by displaying the time series values ​​of the measured value 111 and the current detection value 112 side by side on the display unit 52, the decrease in the visibility of the measured value 111 and the current detection value 112 can be suppressed.

[0084] Furthermore, in this embodiment, as described above, the analysis device 102 (control unit 54) is configured such that, in addition to displaying the time series values ​​of the measured value 111 and the current detection value 112, the display unit 52 also displays sample hole information 52b indicating the multiple sample holes 70a and 71a at multiple configuration positions as the objects to be measured, and arrangement table information 52c indicating the measurement order of the objects to be measured in the multiple sample holes 70a and 71a respectively. With this configuration, in addition to displaying the measured value 111 and the current detection value 112, the sample hole information 52b and the arrangement table information 52c are also displayed, thus allowing the operator to confirm the information of the objects to be measured currently being measured. Therefore, if an abnormality occurs in the measured value 111, it is easy to confirm which sample hole 70a or 71a the measurement was performed on when the abnormality occurred.

[0085] Furthermore, in this embodiment, as described above, the analysis device 102 (control unit 54) is configured to switch between displaying the time series values ​​of the measured value 111 and the current detection value 112 on the display unit 52, and displaying the sample hole information 52b and the arrangement table information 52c in addition to displaying the time series values ​​of the measured value 111 and the current detection value 112, based on a switching operation received by the operation unit 51 for receiving input operations from the operator. With this configuration, the operator can easily switch between displaying the measured value 111 and the current detection value 112, and displaying the sample hole information 52b and the arrangement table information 52c in addition to displaying the measured value 111 and the current detection value 112, by operating the operation unit 51. Additionally, the measured value 111 and the current detection value 112 can be magnified and displayed by switching from a state displaying the sample hole information 52b and the arrangement table information 52c in addition to displaying the measured value 111 and the current detection value 112, to a state displaying only the measured value 111 and the current detection value 112. Therefore, the visibility of the measured value 111 and the current detection value 112 can be easily improved by operating the operation unit 51.

[0086] Furthermore, in this embodiment, as described above, the current detection unit 21 detects the current flowing through each of the plurality of electrodes 65a to 65d arranged in the flow path 61, and the analysis device 102 (control unit 54) is configured to display the time series values ​​of the plurality of current detection values ​​112 corresponding to the current flowing through each of the plurality of (4) electrodes 65a to 65d in a recognizable manner on the display unit 52 using different colors. With this structure, the operator can easily visually distinguish and confirm the current flowing through each of the plurality of (4) electrodes 65a to 65d. Therefore, the operator can distinguish and confirm the current detection values ​​112 of the detected current in more detail.

[0087] Furthermore, in this embodiment, as described above, the flow path 61 includes a preparation flow path 63 for guiding the test object to the separation flow path 62. The current detection unit 21 detects the current flowing through each of the multiple electrodes 65a to 65d, which are respectively disposed at both ends of the separation flow path 62 (liquid reservoirs 64c and 64d) and both ends of the preparation flow path 63 (liquid reservoirs 64a and 64b). The analysis device 102 (control unit 54) is configured such that, during the measurement of the test object and during the introduction of the test object into the preparation flow path 63, the display unit 52 displays the time series values ​​of multiple (4) current detection values ​​112 corresponding to the current flowing through each of the multiple electrodes 65a to 65d, and the time series values ​​of the measurement values ​​111. With this structure, in addition to confirming the current detection value 112 during the measurement process of separating the test object by electrophoresis, it is also possible to confirm the current detection value 112 in case of an abnormality occurring during the introduction of the test object into the preparation flow path 63. Therefore, if an abnormality occurs in the measured value 111, the operator can confirm whether an abnormality occurred during the preparation stage of the measurement.

[0088] Furthermore, in this embodiment, as described above, the measuring unit 30 measures the sample separated by electrophoresis in the flow path 61 of each of the multiple chips 60a-60c (flow path members) having an internal flow path 61. The current detection unit 21 detects the current flowing through the flow path 61 of each of the multiple chips 60a-60c. The analysis device 102 (control unit 54) is configured to have the display unit 52 display the time sequence values ​​of the measured value 111 and the current detection value 112 for each of the multiple chips 60a-60c. With this structure, even when measurements are performed simultaneously in the flow path 61 of the multiple chips 60a-60c, the operator can easily distinguish and confirm whether any abnormality has occurred in each flow path 61.

[0089] (Effects of the electrophoretic analysis method and electrophoretic analysis procedure in this embodiment)

[0090] In the electrophoretic analysis method and electrophoretic analysis procedure 53a of this embodiment, the following effects can be obtained.

[0091] In the electrophoretic analysis method and electrophoretic analysis procedure 53a of this embodiment, by the configuration described above, during the measurement of the test object, the display unit 52 displays the time series values ​​of the measured value 111 and the time series values ​​of the current detection value 112. Here, if both the measured value 111 and the current (current detection value 112) are abnormal, possibilities such as abnormalities in the concentration of the test object itself, abnormalities in the power supply section (voltage application section 20, wiring components, or electrodes 65a-65d, etc.) used for the flowing current, or abnormalities in the process of filling the flow path 61 with the test object (abnormalities in the supply section 10) are considered. On the other hand, if the measured value 111 is abnormal but the current (current detection value 112) is not abnormal, the possibility of deterioration of the components (chips 60a-60c) on which the flow path 61 is formed is considered. Therefore, as described above, during the measurement of the object being measured, the display unit 52 displays the time series values ​​of the measured value 111 and the time series values ​​of the current detection value 112. This allows for visual identification of the display unit 52 to confirm whether either the measured value 111 or the current (current detection value 112) has become abnormal. Thus, it is possible to confirm whether the current has become abnormal when the measured value 111 becomes abnormal, and thus, factors contributing to the abnormality of the measured value 111 can be identified from the following: abnormalities in the concentration of the object being measured, abnormalities in the power supply components (voltage application unit 20, wiring components, or electrodes 65a-65d, etc.) used for the flowing current, or abnormalities in the process of filling the flow path 61 (abnormalities in the supply unit 10); and the possibility of deterioration in the components forming the flow path 61 (chips 60a-60c). As a result, an electrophoretic analysis method and electrophoretic analysis procedure 53a can be provided that reduces the workload of operators in identifying factors contributing to abnormalities when the measured value 111 obtained by electrophoresis becomes abnormal.

[0092] [Variation Example]

[0093] Furthermore, it should be understood that the embodiments disclosed herein are illustrative in all respects and not restrictive. The scope of the invention is shown by the claims rather than by the description of the foregoing embodiments, and includes all modifications (variations) within the meaning and scope equivalent to the claims.

[0094] For example, in the above embodiment, an example is shown where an electrophoresis apparatus 101 for performing electrophoresis-based measurements and an analysis apparatus 102 for displaying measured values ​​111, current detection values ​​112, and voltage detection values ​​113 are independently provided; however, the present invention is not limited thereto. For example, the electrophoresis apparatus 101 for performing electrophoresis-based measurements may also be configured to display measured values ​​111, current detection values ​​112, and voltage detection values ​​113. Alternatively, a display device (display unit) for displaying measured values ​​111, current detection values ​​112, and voltage detection values ​​113 may be provided independently of the electrophoresis apparatus 101 and the analysis apparatus 102.

[0095] Furthermore, in the above embodiment, an example was shown where the display unit 52 displays the time series values ​​of the measured value 111, the current detection value 112, and the voltage detection value 113. However, the present invention is not limited to this. For example, the time series values ​​of the measured value 111 and the current detection value 112 may be displayed, but the time series value of the voltage detection value 113 may not be displayed.

[0096] Furthermore, in the above embodiment, an example was shown in which the display unit 52 displays the time series values ​​of the measured values ​​111, current detection values ​​112, and voltage detection values ​​113 throughout a common measurement period starting from the time point when the voltage is applied from the voltage application unit 20. However, the present invention is not limited to this. For example, it is also possible to display the time series values ​​of the current detection values ​​112 and voltage detection values ​​113 starting from the time point when the voltage is applied from the voltage application unit 20, and to display the time point when electrophoresis begins in the separation flow path 62 (…). Figure 5 and Figure 6 The measured value 111 is the time series value starting from time point T1. Alternatively, it can be the time series values ​​of the measured value 111, current detection value 112, and voltage detection value 113, respectively, displayed throughout the entire common measurement period during the measurement of the object being measured (starting from the time point when electrophoresis begins in the separation flow path 62). Alternatively, the measured value 111, current detection value 112, and voltage detection value 113 can be displayed as time series values ​​showing different periods.

[0097] Furthermore, in the above embodiment, an example is shown in which the time series values ​​of the measured value 111, the current detection value 112, and the voltage detection value 113 are displayed side by side in a horizontal direction, but the present invention is not limited thereto. For example, the time series values ​​of the measured value 111, the current detection value 112, and the voltage detection value 113 may also be displayed in a vertical direction (vertical). Alternatively, the time series values ​​of the measured value 111, the current detection value 112, and the voltage detection value 113 may be displayed superimposed. In this case, it is preferable to display only the superimposed time series values ​​of the current detection value 112 and the voltage detection value 113.

[0098] Furthermore, in the above embodiment, an example is shown where, in addition to displaying the time series values ​​of the measured value 111, the current detection value 112, and the voltage detection value 113, the sample hole information 52b and the arrangement table information 52c are also displayed; however, the present invention is not limited thereto. For example, either the sample hole information 52b or the arrangement table information 52c may be displayed together with the time series values ​​of the measured value 111, the current detection value 112, and the voltage detection value 113.

[0099] Furthermore, in the above embodiment, an example of switching the display based on a switching operation received by the operation unit 51 is shown, but the present invention is not limited thereto. For example, it is also possible that the display is not switched when the sample hole information 52b and the arrangement table information 52c are displayed in addition to the time series values ​​of the measured value 111, the current detection value 112, and the voltage detection value 113. Alternatively, the display can be switched by deleting one of the sample hole information 52b and the arrangement table information 52c.

[0100] Furthermore, in the above embodiment, an example was shown displaying the time series values ​​of four current detection values ​​112 of the current flowing through each of the plurality of electrodes 65a to 65d, but the present invention is not limited thereto. For example, the time series value of one current detection value 112 of the current flowing through each of the plurality of electrodes 65a to 65d may also be displayed. Alternatively, two current detection values ​​112 may be displayed in such a manner that the current of the separation flow path 62 and the current of the preparation flow path 63 are shown one by one. Similarly, one voltage detection value 113 or two voltage detection values ​​113 may be displayed instead of four voltage detection values ​​113.

[0101] Furthermore, in the above embodiments, an example is shown where a preparation flow path 63 for guiding the measurement object to the separation flow path 62 is provided in the chips 60a-60c (flow path components), but the present invention is not limited thereto. For example, the chips 60a-60c (flow path components) may be configured to have only the separation flow path 62 and not include the preparation flow path 63. Alternatively, the preparation flow path 63 may be configured to intersect the separation flow path 62 in a T-shape, rather than intersecting the separation flow path 62 in a through-type shape (cross shape).

[0102] Furthermore, in the above embodiment, an example was shown where the electrophoresis apparatus 101 was configured to measure each of the multiple (3) chips 60a to 60c (flow path components), but the present invention is not limited thereto. For example, measurement of one or two chips (flow path components) may be performed, or measurement of four or more chips may be performed. In addition, even when the electrophoresis apparatus 101 is configured to measure each of the three chips 60a to 60c (flow path components), it may be configured to select only one or two chips for measurement.

[0103] Furthermore, in the above embodiment, an example of configuring the electrophoresis apparatus 101 to perform microchip electrophoresis has been shown, but the present invention is not limited thereto. For example, it may also be configured to perform capillary electrophoresis without using a microchip.

[0104] Furthermore, while the above embodiments illustrate an example of measuring the separation (mobility) of the analyte using fluorescence detection, the present invention is not limited thereto. For example, the separated components of the analyte can also be detected by staining with a reagent.

[0105] [Way]

[0106] Those skilled in the art will understand that the exemplary embodiments described above are specific examples of the following approaches.

[0107] (Project 1)

[0108] An electrophoresis system includes an electrophoresis apparatus comprising: a measuring unit for measuring the measured object after it has been separated by electrophoresis in a flow path including a separation flow path for separating the measured object; and a current detection unit for detecting the current flowing through the flow path.

[0109] The electrophoresis system is configured such that, during the measurement of the object being measured, the display unit displays the time series values ​​of the measured values ​​of the object measured by the measurement unit and the time series values ​​of the current detected by the current detection unit.

[0110] (Project 2)

[0111] According to the electrophoresis system described in Project 1, among which,

[0112] It also includes an analysis device for acquiring the measured values ​​and the current detection values.

[0113] The analysis device is configured such that, during the measurement of the object being measured, the time series values ​​of the measured value and the current detection value are updated in real time and displayed on the display unit.

[0114] (Project 3)

[0115] According to the electrophoresis system described in Project 1 or 2, among which,

[0116] The electrophoresis apparatus further includes a voltage detection unit for detecting the voltage applied to the flow path.

[0117] The electrophoresis system is configured such that, in addition to displaying the time series values ​​of the measured value and the current detection value on the display unit, it also displays the time series values ​​of the voltage detection value detected by the voltage detection unit during the measurement of the measured object.

[0118] (Project 4)

[0119] Based on the electrophoresis system described in any of items 1 to 3, among which,

[0120] The electrophoresis system is configured such that the time series values ​​of the measured values ​​and the current detection values ​​over the entire period of a common measurement period are displayed as waveforms on the display unit.

[0121] (Project 5)

[0122] According to the electrophoresis system described in any one of items 1 to 4, among which,

[0123] The electrophoresis system is configured to display the time series values ​​of the measured value and the current detection value side by side on the display unit.

[0124] (Project 6)

[0125] According to the electrophoresis system described in any one of items 1 to 5, among which,

[0126] The electrophoresis system is configured such that, in addition to displaying the time series values ​​of the measured values ​​and the current detection values, the display unit also displays sample well information representing multiple sample wells and arrangement table information representing the measurement order, wherein the multiple sample wells are multiple configuration positions for arranging the measurement objects, and the measurement order is the measurement order of the measurement objects respectively arranged in the multiple sample wells.

[0127] (Project 7)

[0128] According to the electrophoresis system described in Project 6, among which,

[0129] The electrophoresis system is configured to switch between displaying the time series values ​​of the measured values ​​and the current detection values ​​on the display unit and displaying the sample well information and the arrangement table information in addition to displaying the time series values ​​of the measured values ​​and the current detection values, based on a switching operation received by the operation unit for accepting input operations from the operator.

[0130] (Project 8)

[0131] According to the electrophoresis system described in any one of items 1 to 7, among which,

[0132] The current detection unit detects the current flowing through each of the multiple electrodes arranged in the flow path.

[0133] The electrophoresis system is configured to display, on the display unit, the time series values ​​of multiple current detection values ​​corresponding to the current flowing through each of the plurality of electrodes, superimposed with different colors in a distinguishable manner.

[0134] (Project 9)

[0135] According to the electrophoresis system described in any one of items 1 to 8, among which,

[0136] The flow path also includes a preparation flow path for guiding the measured object to the separation flow path.

[0137] The current detection unit detects the current flowing through each of the multiple electrodes respectively disposed at both ends of the separation flow path and both ends of the preparation flow path.

[0138] The electrophoresis system is configured such that, during the measurement of the object being measured and during the introduction of the object into the preparation flow path, the display unit displays the time series values ​​of a plurality of current detection values ​​corresponding to the current flowing through each of the plurality of electrodes, as well as the time series values ​​of the measured values.

[0139] (Project 10)

[0140] According to the electrophoresis system described in any one of items 1 to 9, among which,

[0141] The measuring unit measures the object to be measured, which has been separated by electrophoresis in the flow path of each of the plurality of flow path components having the flow path internally arranged.

[0142] The current detection unit detects the current flowing through each of the plurality of flow path components.

[0143] The electrophoresis system is configured such that the display unit displays the time series values ​​of the measured values ​​and the current detection values ​​for each of the plurality of flow path components.

[0144] (Project 11)

[0145] An electrophoretic analysis method includes the following steps:

[0146] A measurement value is obtained by measuring the test object that has been separated by electrophoresis in a flow path including a separation flow path for separating the test object;

[0147] Acquire the current detection value of the current flowing through the flow path; and

[0148] During the measurement of the object being measured, the display unit displays the time series values ​​of the measured values ​​and the time series values ​​of the current detection values.

[0149] (Project 12)

[0150] An electrophoretic analysis procedure that causes a computer to perform the following steps:

[0151] A measurement value is obtained by measuring the test object that has been separated by electrophoresis in a flow path including a separation flow path for separating the test object;

[0152] Acquire the current detection value of the current flowing through the flow path; and

[0153] During the measurement of the object being measured, the display unit displays the time series values ​​of the measured values ​​and the time series values ​​of the current detection values.

Claims

1. An electrophoresis system, comprising an electrophoresis apparatus, The electrophoresis apparatus includes: The measuring unit measures the test object that has been separated by electrophoresis in a flow path including a separation flow path for separating the test object; And a current detection unit, which detects the current flowing through the flow path. The electrophoresis system is configured such that, during the measurement of the object being measured, a display unit shows the time series values ​​of the measured values ​​of the object measured by the measurement unit and the time series values ​​of the current detected by the current detection unit. The electrophoresis system is configured such that, in addition to displaying the time series values ​​of the measured values ​​and the current detection values, the display unit also displays sample well information representing multiple sample wells, which are multiple configuration positions for arranging the measured object.

2. The electrophoresis system according to claim 1, wherein, It also includes an analysis device for acquiring the measured values ​​and the current detection values. The analysis device is configured such that, during the measurement of the object being measured, the time series values ​​of the measured value and the current detection value are updated in real time and displayed on the display unit.

3. The electrophoresis system according to claim 1, wherein, The electrophoresis apparatus further includes a voltage detection unit for detecting the voltage applied to the flow path. The electrophoresis system is configured such that, in addition to displaying the time series values ​​of the measured value and the current detection value on the display unit, it also displays the time series values ​​of the voltage detection value detected by the voltage detection unit during the measurement of the measured object.

4. The electrophoresis system according to claim 1, wherein, The electrophoresis system is configured such that the time series values ​​of the measured values ​​and the current detection values ​​over the entire period of a common measurement period are displayed as waveforms on the display unit.

5. The electrophoresis system according to claim 1, wherein, The electrophoresis system is configured to display the time series values ​​of the measured value and the current detection value side by side on the display unit.

6. The electrophoresis system according to claim 1, wherein, The electrophoresis system is configured such that, in addition to displaying the time series values ​​of the measured values ​​and the current detection values, the display unit also displays a schedule indicating the measurement order, which is the measurement order of the measured objects respectively arranged in the plurality of sample wells.

7. The electrophoresis system according to claim 6, wherein, The electrophoresis system is configured to switch between displaying the time series values ​​of the measured values ​​and the current detection values ​​on the display unit and displaying the sample well information and the arrangement table information in addition to displaying the time series values ​​of the measured values ​​and the current detection values, based on a switching operation received by the operation unit for accepting input operations from the operator.

8. The electrophoresis system according to claim 1, wherein, The current detection unit detects the current flowing through each of the multiple electrodes arranged in the flow path. The electrophoresis system is configured to display the time series values ​​of multiple current detection values ​​corresponding to the current flowing through each of the plurality of electrodes on the display unit in a recognizable manner using different colors.

9. The electrophoresis system according to claim 1, wherein, The flow path also includes a preparation flow path for guiding the measured object to the separation flow path. The current detection unit detects the current flowing through each of the multiple electrodes respectively disposed at both ends of the separation flow path and both ends of the preparation flow path. The electrophoresis system is configured such that, during the measurement of the object being measured and during the introduction of the object into the preparation flow path, the display unit displays the time series values ​​of a plurality of current detection values ​​corresponding to the current flowing through each of the plurality of electrodes, as well as the time series values ​​of the measured values.

10. The electrophoresis system according to claim 1, wherein, The measuring unit measures the object to be measured, which has been separated by electrophoresis in the flow path of each of the plurality of flow path components having the flow path internally arranged. The current detection unit detects the current flowing through each of the plurality of flow path components. The electrophoresis system is configured such that the display unit displays the time series values ​​of the measured values ​​and the current detection values ​​for each of the plurality of flow path components.

11. An electrophoretic analysis method, comprising the following steps: A measurement value is obtained by measuring the test object that has been separated by electrophoresis in a flow path including a separation flow path for separating the test object; Obtain the current detection value of the current flowing through the flow path; as well as During the measurement of the object being measured, the display unit shows the time series values ​​of the measured values ​​and the time series values ​​of the current detection values. In the step of displaying the time series values ​​of the measured values ​​and the time series values ​​of the current detection values ​​on the display unit, in addition to displaying the time series values ​​of the measured values ​​and the current detection values ​​respectively, the display unit also displays sample hole information representing multiple sample holes, which are multiple configuration positions for configuring the measured object.

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