Manganese-resistant and high-efficiency manganese-removing bacteria and application thereof

Abstract

The application belongs to the technical field of microorganisms, and discloses a manganese-tolerant and high-efficiency manganese-removing bacterium and application thereof; the manganese-tolerant and high-efficiency manganese-removing bacterium is named Serratia marcescens QZB-1, has been preserved in the Guangdong Microbial Culture Collection Center, and has a preservation number of GDMCC No: 61777 and a preservation date of July 21, 2021; the manganese-tolerant and high-efficiency manganese-removing bacterium QZB-1 is screened and purified from Guangxi acid red soil; after being cultured at 35 DEG C and 150 r / min for 48 h under the condition of pH 5.5, the removal rate of the manganese-tolerant and high-efficiency manganese-removing bacterial strain QZB-1 to 18 mM Mn (II) is optimal; the manganese-tolerant and high-efficiency manganese-removing bacterium QZB-1 screened and purified by the application can tolerate up to 364 mM Mn (II), and the removal rate of the bacterium to 18 mM Mn (II) is as high as 98.4%.

Description

Technical Field

[0001] The invention belongs to the technical field of microorganisms, and in particular relates to manganese-resistant and highly efficient manganese-removing bacteria and applications thereof. Background Art

[0002] Manganese currently accounts for 0.085% of the elements in the Earth's crust and lithosphere, so the Mn(II) content in normal soil is extremely low. However, in the south, where acidic soils (pH < 5.5) are widespread, excessive Mn(II) dissolved in the soil can disrupt plant cell metabolism, hindering the absorption and transfer of other mineral elements, and becoming a major factor limiting normal crop growth and yield. In recent years, the acidification of farmland soils in southern China has gradually increased, along with Mn(II) content, leading to increasing manganese toxicity in the soil. Finding appropriate detoxification methods is urgent.

[0003] Traditional heavy metal remediation methods such as chemical precipitation, ion exchange, filtration, membrane separation, and redox methods have the disadvantages of high cost, unstable treatment effect, and easy to cause secondary pollution. Bioremediation technology has the advantages of low cost, simple operation, good treatment effect and no secondary pollution to the environment. It can not only quickly and effectively remove heavy metals in the soil without changing the physical and chemical properties of the environment, but also is an environmentally friendly remediation technology. Studies have found that microorganisms that have been in heavy metal contaminated soil for a long time can not only resist the stress of heavy metals, but also reduce the content of heavy metals in the soil through surface adsorption, extracellular precipitation, and intracellular detoxification, thereby achieving the effect of repairing pollution. Because microorganisms reproduce quickly, have a large biomass, and can quickly remove heavy metals, microbial remediation technology has become a hot topic of research in recent years.

[0004] Microorganisms can reduce the toxicity of Mn(II) in soil by adsorbing or oxidizing Mn(II) to form manganese oxide precipitates. Different bacteria utilize distinct mechanisms for Mn conversion, including surface adsorption, direct oxidation, and indirect oxidation. Currently, the enzymes believed to be involved in Mn oxidation catalysis include multicopper oxidases, manganese peroxidases, and manganese catalases. However, due to the diverse and complex nature of Mn-tolerant microorganisms and the varying types of enzymes involved in their oxidation, there is no consensus on the biological mechanisms of Mn(II) oxidation by Mn-tolerant bacteria. Several Mn-tolerant bacteria with high Mn(II) removal efficiency have been reported in genera such as Bacillus, Pseudomonas, Pedomicrobium, Gallionella, Leptothrix, and Aminobacter. Of these, only Pseudomonas, Bacillus, Pedomicrobium, and Leptothrix serve as model strains. Therefore, screening for novel Mn-tolerant bacteria and exploring their Mn(II) oxidation mechanisms are crucial.

[0005] Through the above analysis, the problems and defects of the existing technology are as follows:

[0006] (1) Traditional heavy metal remediation methods such as chemical precipitation, ion exchange, filtration, membrane separation, and redox methods have the disadvantages of high cost, unstable treatment effects, and easy to cause secondary pollution.

[0007] (2) Due to the complexity and diversity of manganese-resistant microorganisms, the types of enzymes that catalyze oxidation in their bodies are not the same. Therefore, there is no unified conclusion in the existing technology on the biological oxidation mechanism of Mn(II) by manganese-resistant bacteria. Summary of the Invention

[0008] In view of the problems existing in the prior art, the present invention provides a manganese-resistant and highly efficient manganese-removing bacterium and its application.

[0009] The present invention is achieved in this way. A manganese-resistant and efficient manganese-removing bacterium is classified and named Serratia marcescens QZB-1, which has been deposited in the Guangdong Provincial Microbial Culture Collection Center with a deposit number of GDMCC No: 61777 and a deposit date of July 21, 2021.

[0010] Furthermore, the manganese-tolerant and highly efficient manganese-removing bacterium QZB-1 was screened and purified from the acidic red soil in Guangxi;

[0011] After culturing for 48 h at pH 5.5, 35°C and 150 r / min, the manganese-resistant and efficient manganese-removing bacterial strain Serratia marcescens QZB-1 had the best removal rate for 18 mM Mn(II).

[0012] Another object of the present invention is to provide a method for identifying the tolerance of the manganese-resistant and efficient manganese-removing bacteria to Mn(II) and the influence of environmental factors on the removal of Mn(II). The method for identifying the tolerance of the manganese-resistant and efficient manganese-removing bacteria to Mn(II) and the influence of environmental factors on the removal of Mn(II) comprises: inoculating the strain QZB-1 activated in the LB liquid medium at pH 7.1 into the LB liquid medium at pH 5.5 containing 0-364mM Mn(II), and measuring the OD value of the bacterial solution after culturing for 48 hours. 600 , pH and the content of Mn(II) in the culture medium;

[0013] Among them, OD 600 The pH was determined by a UV spectrophotometer at a wavelength of 600 nm, the pH was determined by a glass electrode method, and the Mn(II) content was determined by an inductively coupled plasma emission spectrometer.

[0014] Further, the initial OD 600 Set to 0.1.

[0015] Furthermore, the culture conditions were 150 r / min and 30°C.

[0016] Furthermore, the determination method of different forms of manganese includes: taking 5 mL of bacterial suspension, centrifuging at 10000 r / min and 4 ° C for 10 min, filtering the supernatant with a 0.45 μm filter membrane, and measuring the Mn (II) in the solution, which is the remaining Mn (II) concentration in the culture medium; the centrifuged bacteria are re-dissolved with an equal volume of 50 mmol / LCuSO4 solution, and the reaction is shaken overnight; the adsorbed Mn (II) is completely absorbed by Cu 2 + After displacement, centrifuge at 10,000 r / min and 4°C for 10 minutes, filter the supernatant with a 0.45 μm filter membrane, and measure the Mn(II) in the solution, which is the concentration of adsorbed Mn(II). Then, treat the precipitate after centrifugation with an equal volume of 20 mmol / L hydroxylamine hydrochloride for more than 10 hours to reduce the high-valent manganese oxide to Mn(II). Centrifuge at 10,000 r / min and 4°C for 10 minutes, filter the supernatant with a 0.45 μm filter membrane, and measure the Mn(II) in the solution, which is the concentration of Mn(II) biooxidized to biooxidized manganese oxide.

[0017] Furthermore, the method for identifying the tolerance of manganese-resistant and efficient manganese-removing bacteria to Mn(II) and the influence of environmental factors on the removal of Mn(II) also includes: inoculating the strain QZB-1 activated in LB liquid medium at pH 7.1 into LB liquid medium containing 18mM Mn(II) and culturing for 2 days, analyzing the removal characteristics of Mn(II) by the strain QZB-1 under different temperature, pH and dissolved oxygen conditions, and regularly sampling to measure OD 600 value, pH and the content of Mn(II) in the bacterial solution.

[0018] Further, the initial OD 600 Set to 0.1.

[0019] Furthermore, different temperatures were set to 10, 20, 30, 35 and 40°C, pH was set to 3.0, 4.0, 5.0, 5.5 and 6.0, and dissolved oxygen was set to 0, 50, 100, 150 and 200 r / min.

[0020] Another object of the present invention is to provide an application of the manganese-resistant and efficient manganese-removing bacteria in manganese removal, wherein the application of the manganese-resistant and efficient manganese-removing bacteria in manganese removal is to remove Mn(II) in acidic soil.

[0021] In combination with the above technical solutions and the technical problems solved, the advantages and positive effects of the technical solutions to be protected by the present invention are as follows:

[0022] First, in view of the technical problems existing in the above-mentioned prior art and the difficulty of solving these problems, we closely combine the technical solutions to be protected by the present invention and the results and data during the research and development process, and conduct a detailed and in-depth analysis of how the technical solutions of the present invention solve the technical problems and some creative technical effects brought about by solving the problems. The specific description is as follows:

[0023] The invention screens and purifies a manganese-resistant bacterium Serratiamarcescens QZB-1 from acidic red soil in Guangxi, and analyzes the strain's tolerance to Mn(II) and the influence of environmental factors on the removal of Mn(II). In a manganese-free culture medium, the strain entered the stationary phase after 12 hours of culture; in culture media with different initial manganese concentrations, the stationary phase of strain QZB-1 was delayed to varying degrees; at a Mn(II) concentration of 364 mM, the growth of strain QZB-1 was strongly inhibited by manganese, but the strain continued to grow after 28 hours; the strain had the best manganese removal effect at a Mn(II) concentration of 18 mM, with a manganese removal rate of 91.8%; the effects of different initial pH values ​​on the manganese removal effect of strain QZB-1 were analyzed at a Mn(II) concentration of 18 mM, and the strain had the highest manganese removal rate of 94.4% at an initial pH of 5.5; the effect of temperature on the manganese removal effect of strain QZB-1 was analyzed under the conditions of a Mn(II) concentration of 18 mM and a pH of 5.5. The strain QZB-1 had the largest biomass and the highest manganese removal rate of 98.4% at 35°C.

[0024] In addition, the effects of dissolved oxygen content in the culture medium on the growth and manganese removal efficiency of strain QZB-1 were studied under the conditions of 18 mM Mn(II) concentration, pH 5.5, and 35°C. Within the rotational speed range of 0 to 150 r / min, the bacterial concentration increased with increasing dissolved oxygen, and the manganese removal rate also increased accordingly. The strain grew best at a rotational speed of 150 r / min, with the highest manganese removal rate reaching 98.4%. Within 24 hours of growth, the free manganese content in the culture medium decreased rapidly, and by 24 hours, 92.2% of the Mn(II) had been removed. The contents of adsorbed manganese and biooxidized manganese increased simultaneously within 24 hours, with the adsorbed manganese content exceeding the biooxidized content. The mechanism of manganese removal by strain QZB-1 involves both adsorption and oxidation, with adsorption being the primary mechanism for removing Mn(II) from the culture medium.

[0025] Second, considering the technical solution as a whole or from the perspective of the product, the technical effects and advantages of the technical solution to be protected by the present invention are described in detail as follows:

[0026] Experimental results show that the manganese-resistant and efficient manganese-removing bacteria Serratiamarcescens QZB-1 provided by the present invention can tolerate up to 20,000 mg / L of Mn(II); after culturing for 48 hours under pH 5.5, 35°C and 150 r / min, the strain Serratiamarcescens QZB-1 has a removal rate of 18mM Mn(II) of up to 98.4%; at the same time, the strain QZB-1 removes Mn(II) through adsorption and oxidation, with adsorption being dominant.

[0027] Third, as auxiliary evidence for the inventiveness of the claims of the present invention, it is also reflected in the following important aspects:

[0028] The expected benefits and commercial value of the technical solution of the present invention after transformation are: it can solve the problem of manganese stress in soil and manganese pollution in water bodies, and can be efficiently used after being made into a bacterial agent, thereby obtaining huge commercial value. BRIEF DESCRIPTION OF THE DRAWINGS

[0029] In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the following briefly introduces the drawings required for use in the embodiments of the present invention. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without any creative work.

[0030] Figure 1 This is a flow chart of manganese-resistant and highly efficient manganese-removing bacteria and their applications provided by an embodiment of the present invention;

[0031] Figure 2 1 is a growth curve of strain QZB-1 under different Mn(II) concentrations provided in an embodiment of the present invention;

[0032] Figure 3 This is a diagram showing the manganese removal effect of strain QZB-1 at different Mn(II) concentrations provided in an embodiment of the present invention;

[0033] Figure 4 Schematic diagram showing the effect of different initial pH values ​​on manganese removal efficiency provided by an embodiment of the present invention;

[0034] Figure 5 Schematic diagram showing the effect of different initial temperatures on manganese removal rate provided by an embodiment of the present invention;

[0035] Figure 6 Schematic diagram showing the effect of dissolved oxygen content in the culture medium on the growth and manganese removal effect of strain QZB-1 provided in an embodiment of the present invention;

[0036] Figure 7 Schematic diagram of the change in manganese concentration in different forms provided by an embodiment of the present invention. DETAILED DESCRIPTION

[0037] In order to make the purpose, technical solutions and advantages of the present invention more clearly understood, the present invention is further described in detail below in conjunction with the embodiments. It should be understood that the specific embodiments described herein are only used to explain the present invention and are not intended to limit the present invention.

[0038] In view of the problems existing in the prior art, the present invention provides a manganese-resistant and highly efficient manganese-removing bacterium and its application. The present invention is described in detail below with reference to the accompanying drawings.

[0039] The manganese-resistant and efficient manganese-removing bacteria provided in the embodiment of the present invention are classified and named Serratia marcescens QZB-1, which has been deposited in the Guangdong Provincial Microbiological Culture Collection Center with the deposit number GDMCC No: 61777 and the deposit date is July 21, 2021.

[0040] The manganese-resistant and efficient manganese-removing bacteria QZB-1 provided in an embodiment of the present invention were screened and purified from the acidic red soil in Guangxi; after being cultured for 48 hours at pH 5.5, 35°C and 150r / min, the manganese-resistant and efficient manganese-removing bacterial strain Serratia marcescens QZB-1 had the optimal removal rate for 18mM Mn(II).

[0041] like Figure 1 As shown, the method provided in the embodiment of the present invention for identifying the tolerance of manganese-resistant and efficient manganese-removing bacteria to Mn(II) and the influence of environmental factors on the removal of Mn(II) includes the following steps:

[0042] S101, inoculating the strain QZB-1 activated in LB liquid medium at pH 7.1 into LB liquid medium at pH 5.5 containing 0-364Mn(II);

[0043] S102, initial OD 600 The OD value of the bacterial solution was determined after the strain QZB-1 was cultured at 150 r / min and 30 °C for 48 h. 600 , pH and the content of Mn(II) in the culture medium;

[0044] S103, measure OD using a UV spectrophotometer at a wavelength of 600 nm 600 The pH was determined by glass electrode method, and the Mn(II) content was determined by inductively coupled plasma emission spectrometry.

[0045] The method for determining different forms of manganese provided in the embodiment of the present invention comprises: taking 5 mL of bacterial suspension and centrifuging at 10000 r / min and 4°C for 10 min, filtering the supernatant with a 0.45 μm filter membrane, and measuring the Mn(II) in the solution, which is the remaining Mn(II) concentration in the culture medium; re-dissolving the centrifuged bacteria with an equal volume of 50 mmol / LCuSO4 solution, and shaking the reaction overnight; the adsorbed Mn(II) is completely absorbed by Cu 2+ After displacement, centrifuge at 10,000 r / min and 4°C for 10 minutes, filter the supernatant with a 0.45 μm filter membrane, and measure the Mn(II) in the solution, which is the concentration of adsorbed Mn(II); treat the precipitate after centrifugation with an equal volume of 20 mmol / L hydroxylamine hydrochloride for more than 10 hours to reduce the high-valent manganese oxide to Mn(II); centrifuge at 10,000 r / min and 4°C for 10 minutes, filter the supernatant with a 0.45 μm filter membrane, and measure the Mn(II) in the solution, which is the concentration of Mn(II) biooxidized to biooxidized manganese.

[0046] The method for identifying the tolerance of manganese-resistant and efficient manganese-removing bacteria to Mn(II) and the influence of environmental factors on the removal of Mn(II) provided in the embodiment of the present invention further comprises: inoculating the strain QZB-1 activated in the LB liquid medium at pH 7.1 into the LB liquid medium containing 18mM Mn(II) and culturing for 2 days, and the initial OD 600 Set to 0.1; analyze the removal characteristics of strain QZB-1 for Mn(II) under different temperature, pH and dissolved oxygen conditions, and take samples regularly to measure OD 600 value, pH and the content of Mn(II) in the bacterial solution.

[0047] The different temperatures provided in the embodiment of the present invention are set to 10, 20, 30, 35 and 40°C in sequence;

[0048] The pH was set to 3.0, 4.0, 5.0, 5.5, and 6.0;

[0049] The dissolved oxygen was set to 0, 50, 100, 150 and 200 r / min in sequence.

[0050] In order to prove the creativity and technical value of the technical solution of the present invention, this section provides application examples of the claimed technical solution on specific products or related technologies.

[0051] The application embodiment of the present invention provides an application of manganese-resistant and efficient manganese-removal bacteria in manganese removal. The application of manganese-resistant and efficient manganese-removal bacteria in manganese removal is to remove Mn(II) in acidic soil and Mn(II) in water.

[0052] The invention screens and purifies a manganese-resistant bacterium Serratiamarcescens QZB-1 from acidic red soil in Guangxi, and analyzes the strain's tolerance to Mn(II) and the influence of environmental factors on the strain's removal of Mn(II).

[0053] The strain QZB-1 activated in LB liquid medium (pH 7.1) was inoculated into LB liquid medium (pH 5.5) containing 0-364 mM Mn(II) (initial OD 600 0.2), 150 r / min, 30 ° C for 48 h and then measure the OD of the bacterial solution 600 , pH and the content of Mn(II) in the culture medium. 600 The concentration of Mn(II) in the solution was determined by an inductively coupled plasma emission spectrometer (ICP-500) at a wavelength of 600 nm.

[0054] Determination of different forms of manganese: 5 mL of bacterial suspension was centrifuged at 10,000 r / min and 4°C for 10 min. The supernatant was filtered with a 0.45 μm filter membrane and the Mn(II) in the solution was measured, which was the remaining Mn(II) concentration in the culture medium. The centrifuged bacteria were re-dissolved with an equal volume of 50 mmol / LCuSO4 solution and the reaction was shaken overnight. The adsorbed Mn(II) was completely absorbed by CuSO4. 2+ After displacement, centrifuge at 10,000 r / min and 4°C for 10 minutes, filter the supernatant with a 0.45 μm filter membrane, and measure the Mn(II) in the solution, which is the concentration of adsorbed Mn(II). Then, treat the precipitate after centrifugation with an equal volume of 20 mmol / L hydroxylamine hydrochloride for more than 10 hours to reduce the high-valent manganese oxide to Mn(II). Centrifuge at 10,000 r / min and 4°C for 10 minutes, filter the supernatant with a 0.45 μm filter membrane, and measure the Mn(II) in the solution, which is the concentration of Mn(II) biooxidized to biooxidized manganese.

[0055] The strain QZB-1 activated in LB liquid medium (pH 7.1) was inoculated into LB liquid medium containing 18 mM Mn(II) (initial OD 600 The removal characteristics of Mn(II) by strain QZB-1 were analyzed under different temperature (10, 20, 30, 35 and 40℃), pH (3.0, 4.0, 5.0, 5.5 and 6.0) and dissolved oxygen (0, 50r / min, 100r / min, 150r / min and 200r / min) conditions. Samples were taken regularly to measure OD 600 value, pH and Mn(II) content in the bacterial solution.

[0056] The growth curves of strain QZB-1 at different initial Mn(II) concentrations are shown in Figure 2. Figure 2 As shown. In manganese-free medium, the strain entered the stationary phase after 12 hours of culture. In medium containing different initial manganese concentrations, the stationary phase of strain QZB-1 was delayed to varying degrees. At 364 mM Mn(II), strain QZB-1 growth was strongly inhibited by manganese, but continued to grow after 28 hours. Figure 3 The results show that the strain QZB-1 has the best manganese removal effect at different initial Mn(II) concentrations after 48 h of culture, with a manganese removal rate of 91.8% when the Mn(II) concentration is 18 mM.

[0057] The effect of different initial pH on the manganese removal efficiency of strain QZB-1 was analyzed at a Mn(II) concentration of 18 mM (see Figure 4 ), the strain had the highest manganese removal rate at the initial pH of 5.5, reaching 94.4%.

[0058] The effect of temperature on the manganese removal efficiency of strain QZB-1 was analyzed under the conditions of Mn(II) concentration of 18 mM and pH 5.5 (see Figure 5 ), strain QZB-1 had the largest biomass and the highest manganese removal rate at 35℃, reaching 98.4%.

[0059] Figure 6 The results showed that the dissolved oxygen content in the culture medium affected the growth and manganese removal efficiency of strain QZB-1 at a Mn(II) concentration of 18 mM, pH 5.5, and 35°C. Within the rotational speed range of 0 to 150 r / min, the bacterial concentration increased with increasing dissolved oxygen, and the manganese removal rate also increased accordingly. The strain grew best at a rotational speed of 150 r / min, with the highest manganese removal rate reaching 98.4%.

[0060] The changes in the content of different forms of manganese in different growth stages of strain QZB-1 are as follows Figure 7 As shown, within 24 hours of growth, the free manganese content in the culture medium decreased rapidly, and by 24 hours, 92.2% of the Mn(II) in the culture medium had been removed. The levels of adsorbed and biooxidized manganese increased simultaneously within 24 hours, with the adsorbed manganese content exceeding the biooxidized content. This suggests that strain QZB-1 removes manganese by both adsorption and oxidation mechanisms, with the strain primarily removing Mn(II) from the culture medium through adsorption. After 24 hours, the free manganese content slowly decreased, while the levels of adsorbed and biooxidized manganese did not increase significantly. This is presumably due to the gradual decline of the bacteria in the culture medium, weakening adsorption and oxidation processes.

[0061] Serratia marcescens QZB-1 can tolerate up to 364 mM Mn(II). After 48 hours of cultivation at pH 5.5, 35°C, and 150 rpm, strain QZB-1 achieved a removal efficiency of 98.4% for 18 mM Mn(II). Strain QZB-1 removes Mn(II) via both adsorption and oxidation, with adsorption being the dominant mechanism.

[0062] like Figure 2 As shown, the steps are as follows: the strain QZB-1 activated in LB liquid medium (pH 7.1) is inoculated into LB liquid medium (pH 5.5) containing 0-364mM Mn (Ⅱ) (initial OD 600 0.2), 150 r / min, 30 ° C for 48 h and then measure the OD of the bacterial solution 600

[0063] like Figure 3 As shown, the steps are as follows: the strain QZB-1 activated in LB liquid medium (pH 7.1) is inoculated into LB liquid medium (pH 5.5) containing 0-364mM Mn (Ⅱ) (initial OD 600 After culturing for 48 h at a speed of 0.2), 150 r / min and 30°C, the pH and the content of Mn(Ⅱ) in the culture medium were determined.

[0064] Steps: The strain QZB-1 activated in LB liquid medium (pH 7.1) was inoculated into LB liquid medium with different pH values ​​(3.0, 4.0, 5.0, 5.5 and 6.0) containing 18 mM Mn (II) (initial OD 600 The OD value of the bacterial solution was determined after culturing at 150 rpm and 30 °C for 48 h. 600 , pH and Mn(Ⅱ) content in culture medium

[0065] like Figure 5 As shown, the steps are as follows: the strain QZB-1 activated in LB liquid medium (pH 7.1) is inoculated into LB liquid medium containing 18mM Mn (Ⅱ) with a pH of 5.5 (initial OD 600 The bacterial solution OD was measured after culturing at different temperatures (10, 20, 30, 35 and 40 ° C) at 150 rpm for 48 h. 600 , pH and the content of Mn(Ⅱ) in the culture medium Figure 6 As shown, the steps are as follows: the strain QZB-1 activated in LB liquid medium (pH 7.1) is inoculated into LB liquid medium containing 18mM Mn (Ⅱ) with a pH of 5.5 (initial OD 600The culture medium was placed in a shaker at 30°C and cultured at different speeds (0, 50 r / min, 100 r / min, 150 r / min and 200 r / min) for 48 h, and the OD of the bacterial solution was measured. 600 , pH and Mn(Ⅱ) content in culture medium

[0066] like Figure 7 As shown, the steps are as follows: the strain QZB-1 activated in LB liquid medium (pH 7.1) is inoculated into LB liquid medium containing 18mM Mn (Ⅱ) with a pH of 5.5 (initial OD 600 The culture medium was placed in a shaker at 30°C and cultured at 150 r / min, and samples were taken regularly to determine the OD value of the bacterial solution. 600 , pH and the content of different forms of Mn in the culture medium. The determination method of different forms of manganese includes: taking 5mL of bacterial suspension, centrifuging at 10000r / min and 4℃ for 10min, filtering the supernatant with a 0.45μm filter membrane and measuring the Mn(II) in the solution, which is the remaining Mn(II) concentration in the culture medium; the centrifuged bacteria are re-dissolved with an equal volume of 50mmol / LCuSO4 solution and shaken overnight; the adsorbed Mn(II) is completely absorbed by Cu 2+ After displacement, centrifuge at 10,000 r / min and 4°C for 10 minutes, filter the supernatant with a 0.45 μm filter membrane, and measure the Mn(II) in the solution, which is the concentration of adsorbed Mn(II). Then, treat the precipitate after centrifugation with an equal volume of 20 mmol / L hydroxylamine hydrochloride for more than 10 hours to reduce the high-valent manganese oxide to Mn(II). Centrifuge at 10,000 r / min and 4°C for 10 minutes, filter the supernatant with a 0.45 μm filter membrane, and measure the Mn(II) in the solution, which is the concentration of Mn(II) biooxidized to biooxidized manganese oxide.

[0067] The above description is only a specific embodiment of the present invention, but the scope of protection of the present invention is not limited thereto. Any modifications, equivalent substitutions and improvements made by any technician familiar with this technical field within the technical scope disclosed by the present invention and within the spirit and principles of the present invention should be covered by the scope of protection of the present invention.

Claims

1. A manganese-resistant and manganese-removing bacterium Serratiamarcescens QZB-1 is characterized by: It has been deposited in Guangdong Provincial Microbiological Culture Collection, with the deposit number GDMCC No: 61777 and the deposit date being July 21, 2021.

2. Use of the manganese-resistant and manganese-removing bacteria according to claim 1 in manganese removal, characterized in that: Removal of Mn from acidic soil and water 2+ .

Images