A multifunctional skin cleansing composition containing biosurfactant and its preparation method and application

Abstract

This invention relates to a multifunctional cleansing composition containing biosurfactants, its preparation method, and its application. The multifunctional cleansing composition includes surfactants and antibacterial agents. The surfactants include biosurfactants: sophora resin and rhamnolipid; phosphate surfactants: cocamidopropyl-PG-dimethylammonium chloride phosphate; zwitterionic surfactants: cocamidopropyl hydroxysulfonate betaine; and amino acid surfactants: sodium lauroyl oat amino acid, sodium cocoaminopropionate, sodium lauroyl sarcosinate, sodium lauryl glutamate, or sodium cocoyl glycinate, or any one or a combination of at least two of these. When used, the multifunctional cleansing composition produces dense foam, has strong cleansing power, is gentle and non-irritating, and possesses antibacterial, anti-inflammatory, anti-mite, antioxidant, conditioning, and repairing effects on the skin. The raw materials used are easily degradable, conform to ecological and environmental protection principles, and can be used for facial and body cleansing.

Description

Technical Field

[0001] The invention belongs to the technical field of daily chemical products, and relates to a multifunctional skin-cleansing composition containing a biosurfactant, and a preparation method and application thereof. Background Art

[0002] Cleansing is the prelude to skin care and a necessary condition for maintaining the normal microbial environment of the skin epidermis. Only when the skin is cleansed well can the skin care be carried out effectively. Some people like to pursue the extreme astringency and excessive cleansing during skin cleansing, which leads to excessive cleansing, destroying the water and oil balance of the skin, thus causing more skin problems. There is a sebum membrane on the outermost layer of our skin. The sebum membrane has important physiological functions for the skin and even the entire organism. The sebum membrane is secreted by the sebaceous glands, flows to the surface of the skin, and spreads out to form a thin layer of "oil film". This "oil film" can reduce the loss of body water, resist the invasion of harmful substances, and play a role in moisturizing the skin. If you over-clean for a long time, it will damage the sebum membrane of the skin, reduce the skin's immunity and weaken the water-locking function, resulting in dry and sensitive skin; secondly, it will damage the stratum corneum of the skin, making the skin thinner and more sensitive.

[0003] Researchers in this field have developed a variety of skin cleansing products. For example, CN104800123A discloses an antibacterial soap-based shower gel and a preparation method thereof. The raw materials for the preparation include thymol, salicylic acid, plant extracts, ethanol, propylene glycol, erythritol, lauric acid, myristic acid, palmitic acid, potassium hydroxide, ethylene glycol distearate, sodium diethylhexyl sulfosuccinate, cocamidopropyl betaine, sodium cocoyl glycinate, polyquaternium-7, polyglycerol fatty acid ester composition, tetrasodium ethylenediaminetetraacetic acid, sodium chloride, citric acid, flavor, preservative and water. The antibacterial soap-based shower gel is mild and skin-friendly, can effectively resist bacteria and moisturize, and is particularly suitable for people with thick stratum corneum, vigorous oil secretion, itchy skin and tinea corporis.

[0004] CN102048668A discloses a shower gel and a preparation method thereof, wherein the shower gel mainly comprises the following components in weight percentage: 12%-20% surfactant, 3%-7% acrylic acid (ester) / stearyl alcohol polyether-20 methacrylate copolymer, 0.5%-1% cocamide DEA, 0.05%-0.3% methylisothiazolinone, 0.3%-0.5% sodium hydroxide, 0.5%-1% dehydrated plant crushed particles and 1%-1.5% sodium chloride. The shower gel of the invention improves the utilization efficiency of plants, and at the same time, due to the addition of stably suspended dehydrated plant crushed particles, it has a certain massage effect on the skin and improves the cleaning effect of the shower gel.

[0005] CN108743509A discloses a hydrating shower gel and a preparation method thereof. The raw materials include the following components in percentage: 2%-7% lauric acid, 5%-8% myristic acid, 0.5%-1.5% stearic acid, 2%-5% potassium hydroxide, 6%-12% cocamide MIPA thickener, 0.5%-1.5% cocoyl glycinate, 5%-10% glycerol, 60%-70% water, 0.2%-1% preservative, and 1-3% each of aloe vera, tea and holly bark extracts. The shower gel is beneficial for tightening skin pores, can make the skin firm and smooth, improves water retention performance, is convenient for skin absorption, and can effectively improve system foam.

[0006] However, these products often cannot take into account both cleaning effect and mildness, and the antibacterial, anti-inflammatory, mite removal, antioxidant, conditioning and skin repair effects of the products are also relatively limited. Therefore, how to develop a cleaning product that is mild and low in irritation, has rich and dense foam, has excellent cleaning power, good antibacterial and mite removal effects, moisturizes the skin, is easy to degrade, and conforms to the concept of ecological protection is still a problem that technical personnel in this field urgently need to solve. Summary of the invention

[0007] In view of the deficiencies of the prior art, the object of the present invention is to provide a multifunctional skin cleansing composition containing a biosurfactant and a preparation method and application thereof.

[0008] The multi-function means that the composition has dense foam and strong cleaning power when used, is mild and non-irritating, and has the effects of antibacterial, anti-inflammatory, mite removal, anti-oxidation, conditioning and repairing the skin.

[0009] In order to achieve the purpose of the invention, the present invention adopts the following technical solutions:

[0010] In a first aspect, the present invention provides a multifunctional skin cleansing composition containing a biosurfactant, wherein the multifunctional skin cleansing composition comprises a surfactant and an antibacterial agent, wherein the surfactant comprises a biosurfactant, a phosphate surfactant, a zwitterionic surfactant and an amino acid surfactant:

[0011] The biosurfactant includes Sophora japonica resin and rhamnolipid, the phosphate surfactant includes cocamidopropyl-PG-dimethylammonium chloride phosphate, the zwitterionic surfactant includes cocamidopropyl hydroxysulfonate, and the amino acid surfactant includes any one of sodium lauroyl oat amino acids, sodium cocoylaminopropionate, sodium lauroyl sarcosinate, sodium lauryl glutamate or sodium cocoyl glycinate, or a combination of at least two thereof;

[0012] The antibacterial agent includes any one of Xanthium sibiricum extract, Hibiscus sylvestris bark extract, Atractylodes macrocephala extract, Acorus calamus extract, Artemisia annua extract, Inula japonica extract, grape polyphenols, oleuropein or polylysine, or a combination of at least two thereof.

[0013] Biosurfactants are a type of degradable surfactant. Biosurfactants not only have the common properties of surfactants such as solubilization, emulsification, wetting, foaming, dispersion, and reduction of surface tension, but also have the advantages of being non-toxic, biodegradable, ecologically safe, temperature-resistant and salt-resistant, and having high surface activity compared to other surfactants produced by chemical synthesis or petroleum refining.

[0014] The present invention creatively adds a biological surfactant to the multifunctional cleansing composition, and compounds it with a phosphate surfactant, a zwitterionic surfactant and an amino acid surfactant to form a composite surfactant, the mildness, foaming effect and cleaning ability of which are all guaranteed.

[0015] Among them, specific surfactant components: Sophora japonica resin, rhamnolipid, cocamidopropyl-PG-dimethylammonium chloride phosphate, cocamidopropyl hydroxysulfonyl betaine and sodium lauroyl oat amino acids can cooperate with each other and have a synergistic effect in improving the foaming effect and cleaning ability of the product. If they are missing or replaced with similar components, the overall effect will be reduced.

[0016] Specifically, the characteristics of each surfactant component are introduced as follows:

[0017] Sophora biosurfactant: Sophora biosurfactant is a glycolipid biosurfactant produced by Candida albicans using sugar and vegetable oil as carbon sources through a fermentation process under certain conditions. Sophora biosurfactant is friendly to aquatic organisms and has excellent general properties such as solubilization, emulsification, wetting, foaming, dispersion, and surface tension reduction. It also has the characteristics of low toxicity or non-toxicity, easy biodegradation, and environmental friendliness. Sophora biosurfactant has outstanding skin mildness, excellent wettability and hard water tolerance, can regulate the affinity between cell surface and insoluble substances, and can help regulate skin microecology while inhibiting bacteria. The extraction process of Sophora biosurfactant resin adopts physical extraction process without organic solvents. All process processes do not have chemical reactions and chemical components added, ensuring that the overall process is green and environmentally friendly. The product can be used in the fields of petroleum, environmental protection, medicine, food, cosmetics, washing, home care, agriculture, feed, etc., and can partially or completely replace the use of chemical synthetic surfactants.

[0018] Rhamnolipid: Rhamnolipid is a biological surfactant with biological metabolic properties produced by Pseudomonas or Burkholderia. It is also a biological surfactant with a long research time and mature application technology. Rhamnolipid also shows good solubility characteristics in alkaline aqueous solution. It has good chemical and biological properties, oil and water amphiphilicity, can reduce water surface tension, and can be used as a wetting agent, emulsifier and foaming agent. Rhamnolipid biosurfactant can be used under extreme conditions of temperature, pH value and salinity, and is non-toxic and biodegradable. In addition to good surface activity, rhamnolipid also has good cell permeability. Because it comes from biological sources, it has good non-toxic and degradable properties. Rhamnolipid can diffuse substances through dispersing and wetting properties. To help retain water, lubricate, generate foam, and act as a cleaning agent. It can maintain the stability of the mixture system, control the viscosity of the product, and emulsify clean grease. It can be widely used in various daily chemical products.

[0019] Cocamidopropyl-PG-dimethylammonium chloride phosphate (Heda / bionic phospholipid CPG): a diester amphoteric surfactant, which belongs to the bionic phospholipid chemical structure and is very similar to the human skin phospholipid structure, so it has good skin-friendly properties. This natural phosphate surfactant has many excellent qualities: mild cleaning power, anti-irritation, high affinity to the skin, a certain repair function for damaged skin and hair, and can replenish the phospholipids and oils lost by the skin. Excellent wettability provides an excellent skin feel after use; significantly reduces the irritation of the entire system; excellent foaming and foam stability; easy to disperse at low temperatures; broad-spectrum antibacterial properties.

[0020] Sodium lauroyl oat amino acids: Amino acid surfactants, a new type of green and environmentally friendly surfactants from renewable biomass, are upgraded products of traditional surfactants. Amino acid surfactants not only have a wide range of biomass raw materials, low toxicity and side effects, mild performance, low irritation and good biodegradability, and green production processes, but also have good emulsification, wetting, solubilization, dispersion, foaming and other properties that have attracted much attention and are gradually being used in many fields such as washing, personal care and food industry. Sodium lauroyl oat amino acids are of natural origin, low odor and low chroma. It has strong foaming power, is resistant to hard water, can form frosty foam, feels extremely mild and comfortable, and is easy to thicken.

[0021] Cocamidopropyl Hydroxysulfobetaine: It is a zwitterionic surfactant. The EWG green grade of cocamidopropyl hydroxysulfobetaine is 1. It has excellent foaming and significant thickening properties, low irritation, good conditioning and low-temperature stability, excellent resistance to hard water, antistatic and biodegradability, and thickening properties for cleaning products. It is widely used in mid-to-high-end facial cleansers, shampoos, and shower gels. It is an ideal material for preparing mild facial cleansers, baby shampoos, and shower gels.

[0022] Preferably, the multifunctional cleansing composition comprises, by weight: 1-10 parts of Sophora resin, 1-10 parts of rhamnolipid, 0.5-5 parts of cocamidopropyl-PG-dimethylammonium chloride phosphate, 1-5 parts of cocamidopropyl hydroxysulfonyl betaine, 10-30 parts of sodium lauroyl oat amino acids and 0.1-20 parts of antibacterial agent.

[0023] Specific numerical values ​​in the above 1-10 parts are, for example, 1 part, 2 parts, 3 parts, 4 parts, 5 parts, 6 parts, 7 parts, 8 parts, 9 parts, 10 parts, etc.

[0024] Specific numerical values ​​in the above 0.5-5 parts include, for example, 0.5 parts, 1 parts, 1.5 parts, 2 parts, 2.5 parts, 3 parts, 3.5 parts, 4 parts, 4.5 parts, 5 parts, etc.

[0025] Specific numerical values ​​in the above 1-5 parts are, for example, 1 part, 1.5 parts, 2 parts, 2.5 parts, 3 parts, 3.5 parts, 4 parts, 4.5 parts, 5 parts, etc.

[0026] Specific values ​​in the above 10-30 parts are, for example, 10 parts, 11 parts, 12 parts, 13 parts, 14 parts, 15 parts, 16 parts, 17 parts, 18 parts, 19 parts, 20 parts, 21 parts, 22 parts, 23 parts, 24 parts, 25 parts, 26 parts, 27 parts, 28 parts, 29 parts, 30 parts, etc.

[0027] The specific values ​​in the above 0.1-20 parts are, for example, 0.1 part, 0.2 part, 0.3 part, 0.4 part, 0.5 part, 0.6 part, 0.7 part, 0.8 part, 0.9 part, 1 part, 2 parts, 3 parts, 4 parts, 5 parts, 6 parts, 7 parts, 8 parts, 9 parts, 10 parts, 11 parts, 12 parts, 13 parts, 14 parts, 15 parts, 16 parts, 17 parts, 18 parts, 19 parts, 20 parts, etc.

[0028] Compared with other ratios, the synergistic effect of the components in the above ratio is better, and the foam density and cleaning ability of the obtained composition are better.

[0029] Preferably, the antibacterial agent comprises Xanthium sibiricum extract, Hibiscus sabdariffa bark extract, Atractylodes macrocephala extract, Acorus calamus extract, Artemisia annua extract, Inula japonica extract, grape polyphenols, oleuropein and polylysine.

[0030] Xanthium sibiricum: The whole plant contains xanthium glycoside, i.e. B-sitosterol glucoside, xanthocyanin, xanthocyanin, which is a stereoisomer of xanthocyanin, as well as caffeic acid and 1,4-dicaffeoylquinic acid. It has pharmacological effects on lowering blood sugar, respiratory system, cardiovascular system, and anti-inflammatory, and can be antibacterial and de-mite.

[0031] Hibiscus: The flowers, fruits, roots, leaves and skin of hibiscus can be used as medicine. It has the effect of preventing and treating viral diseases and lowering cholesterol. Hibiscus flowers contain saponin, isovitexin, saponin, etc. It has a certain inhibitory effect on Staphylococcus aureus and Salmonella typhi, and can treat intestinal wind and bloody diarrhea.

[0032] Atractylodes: The water decoction and volatile oil of Atractylodes have obvious antibacterial effects on Mycobacterium tuberculosis, Staphylococcus aureus, Escherichia coli, Bacillus subtilis, Pseudomonas aeruginosa, Staphylococcus epidermidis, Staphylococcus aureus, hemolytic Streptococcus, Pneumococcus, etc., and the atractylodes ketone contained therein has acaricidal activity.

[0033] Tibetan calamus: The main ingredients are volatile oil, amino acids, sugar, etc. It can relieve asthma, relieve cough and eliminate phlegm; it is antibacterial. The results of modern pharmacological tests show that Tibetan calamus has the effects of tranquilizing the mind, anticonvulsant and cooling down.

[0034] Artemisia annua: The decoction of Artemisia annua has a strong antibacterial effect on Staphylococcus epidermidis, Catarrhalis, Bacillus anthracis, and Corynebacterium diphtheriae. It also has a certain inhibitory effect on Staphylococcus aureus, Pseudomonas aeruginosa, Shigella dysenteriae, and Mycobacterium tuberculosis. Artemisia annua is a plant-based acaricide, which also has a good control effect on phytophagous mites and storage mites.

[0035] Inula lactone and its derivatives contained in the volatile oil of this product have inhibitory effects on Mycobacterium tuberculosis, Shigella dysenteriae, Staphylococcus aureus, Pseudomonas aeruginosa, and common pathogenic skin fungi. The inula lactone volatile oil it contains has anti-parasitic effects.

[0036] Grape polyphenols: also known as grape proanthocyanidins, are a natural plant polyphenol found in grapes. Grape polyphenols have strong antioxidant effects, can delay the oxidation and deterioration of other raw materials, extend the shelf life of the product, and have certain antibacterial and antimicrobial effects.

[0037] Oleuropein: Broad-spectrum antimicrobial ingredient. The most active substance identified so far in olive leaf is oleuropein, a broad-spectrum antimicrobial ingredient that severely interferes with certain amino acid patterns necessary for the growth of a particular virus, bacteria or microorganism. Interferes with viral infection and / or transmission by inactivating the virus or preventing it from molting, budding or budding at the cell membrane. Directly penetrates infected cells and irreversibly inhibits microbial replication. Neutralizes reverse transcriptase and protease products of retroviruses. Olive leaf extract has a complete effect on infectious and malignant microorganisms. It can stop the onset of colds and other viral diseases, fungal, mold and yeast invasions, minor and severe bacterial infections and protozoan parasitic infections. More than just preventative, olive leaf extract offers a safe and effective treatment in the battle against microorganisms.

[0038] Polylysine: It is composed of 25 to 30 lysine residues and has strong antibacterial ability. It can be used as a preservative to preserve food. Polylysine has a broad antibacterial spectrum and has a certain antibacterial effect on Gram-positive bacteria, Gram-negative bacteria, yeasts, and molds in acidic and slightly acidic environments. Polylysine has a very good antibacterial effect on Gram-negative Escherichia coli and Salmonella, which are not easily inhibited by other natural preservatives. It also has an inhibitory effect on heat-resistant Bacillus and some viruses.

[0039] The antibacterial agent prepared by using Xanthium sibiricum, hibiscus bark, Atractylodes lancea, Tibetan calamus, Artemisia annua, Inula japonica, grape polyphenol, oleuropein and polylysine as raw materials has good antibacterial and mite removal effects and guaranteed safety. It is found in the experiment that if one component is missing from the raw materials, the antibacterial and mite removal effects of the antibacterial agent obtained by extraction will be reduced to varying degrees, which may be because the raw material components / the extracts of the raw materials play a synergistic role in antibacterial and mite removal.

[0040] Preferably, the antibacterial agent is prepared by a preparation method comprising the following steps: mixing Xanthium sibiricum, hibiscus bark, Atractylodes lancea, Tibetan calamus, Artemisia annua, and Inula japonica with an extraction solvent to obtain a mixed solution, extracting, filtering, collecting the filtrate, and mixing it with grape polyphenols, oleuropein, and polylysine to obtain the antibacterial agent.

[0041] Preferably, the extraction solvent comprises an ethanol aqueous solution with a volume concentration of 40-60%.

[0042] Specific values ​​of the above 40-60% include, for example, 40%, 42%, 45%, 47%, 50%, 52%, 55%, 57%, 60%, etc.

[0043] Preferably, the components in the mixed solution are calculated by mass percentage as follows: Xanthium sibiricum 0.1%-3%, Hibiscus sylvestris bark 0.1%-3%, Atractylodes lancea 0.1%-3%, Tibetan calamus 0.1%-3%, Artemisia annua 0.1%-3%, Inula japonica 0.1%-3%, and the remainder is extraction reagent.

[0044] The specific values ​​of the above 0.1%-3% are as follows: 0.1%, 0.2%, 0.4%, 0.6%, 0.8%, 1%, 1.2%, 1.4%, 1.6%, 1.8%, 2%, 2.2%, 2.4%, 2.6%, 2.8%, 3%, etc.

[0045] Preferably, the extraction method includes immersion extraction.

[0046] Preferably, the extraction is carried out at 15-40°C, for example, 15°C, 17°C, 20°C, 22°C, 25°C, 27°C, 30°C, 32°C, 35°C, 37°C, 40°C, etc., and the extraction time is 50-90h, for example, 50h, 55h, 60h, 65h, 70h, 75h, 80h, 85h, 90h, etc.

[0047] Preferably, the collecting of the filtrate further comprises concentrating the filtrate, and the volume of the obtained concentrate is 1 / 10-1 / 2 of the volume of the filtrate, for example, 1 / 10, 1 / 9, 1 / 8, 1 / 7, 1 / 6, 1 / 5, 1 / 4, 1 / 3, 1 / 2, etc.

[0048] Preferably, the concentration method includes concentration under reduced pressure.

[0049] Preferably, based on the mass of the filtrate or the concentrated solution being 100%, the mass of the grape polyphenols is 0.1%-1%, the mass of oleuropein is 0.1%-1%, and the mass of polylysine is 1%-10%.

[0050] The specific values ​​of the above 0.1%-1% include 0.1%, 0.2%, 0.3%, 0.4%, 0.5%, 0.6%, 0.7%, 0.8%, 0.9%, 1% and the like.

[0051] The specific values ​​of the above 1%-10% include, for example, 1%, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, 10%, etc.

[0052] Preferably, the multifunctional skin cleansing composition further comprises any one or a combination of at least two of a skin conditioner, an antioxidant, a thickener, a moisturizer, a preservative, or a fragrance or water.

[0053] Preferably, the multifunctional cleansing composition further comprises, by weight, any one of 0.1-10 parts of skin conditioner, 0.01-0.2 parts of antioxidant, 0.1-0.5 parts of thickener, 0.1-5 parts of moisturizer, 0.2-3 parts of preservative, 0.1-0.5 parts of fragrance or 30-90 parts of water, or a combination of at least two thereof.

[0054] Specific values ​​in the above 0.1-10 parts are, for example, 0.1 part, 0.2 part, 0.4 part, 0.6 part, 0.8 part, 1 part, 1.5 parts, 2 parts, 2.5 parts, 3 parts, 3.5 parts, 4 parts, 4.5 parts, 5 parts, 5.5 parts, 6 parts, 6.5 parts, 7 parts, 7.5 parts, 8 parts, 8.5 parts, 9 parts, 9.5 parts, 10 parts, etc.

[0055] Specific values ​​in the above 0.01-0.2 parts are, for example, 0.01 parts, 0.02 parts, 0.03 parts, 0.04 parts, 0.05 parts, 0.06 parts, 0.07 parts, 0.08 parts, 0.09 parts, 0.1 parts, 0.11 parts, 0.12 parts, 0.13 parts, 0.14 parts, 0.15 parts, 0.16 parts, 0.17 parts, 0.18 parts, 0.19 parts, 0.2 parts, etc.

[0056] Specific numerical values ​​in the above 0.1-0.5 parts include, for example, 0.1 parts, 0.15 parts, 0.2 parts, 0.25 parts, 0.3 parts, 0.35 parts, 0.4 parts, 0.45 parts, 0.5 parts, etc.

[0057] Specific values ​​in the above 0.1-5 parts are, for example, 0.1 part, 0.2 part, 0.4 part, 0.6 part, 0.8 part, 1 part, 1.5 parts, 2 parts, 2.5 parts, 3 parts, 3.5 parts, 4 parts, 4.5 parts, 5 parts, etc.

[0058] Specific values ​​in the above 0.2-3 parts are, for example, 0.2 parts, 0.4 parts, 0.6 parts, 0.8 parts, 1 parts, 1.2 parts, 1.4 parts, 1.6 parts, 1.8 parts, 2 parts, 2.2 parts, 2.4 parts, 2.6 parts, 2.8 parts, 3 parts, etc.

[0059] Specific values ​​in the above 30-90 parts include, for example, 30 parts, 35 parts, 40 parts, 45 parts, 50 parts, 55 parts, 60 parts, 65 parts, 70 parts, 75 parts, 80 parts, 85 parts, 90 parts, etc.

[0060] Preferably, the skin conditioning agent comprises any one of phytosterol / octyldodecanol lauroyl glutamate, rhamnose or hydroxypropyl chitosan, or a combination of at least two thereof.

[0061] Phytosterol / Octyldodecyl Lauroyl Glutamate: A sterol derivative, purely plant-derived, similar to the lipids in the skin and hair cell complex, and has a "platelet-like liquid crystal structure" like ceramide, with good moisturizing and emollient effects.

[0062] Rhamnose is a common monosaccharide. In nature, it often exists in combination with other molecules in animals, plants, and polysaccharides. Because it is rich in polysaccharides, pectin, plant glycosides and other effective ingredients, it has a moisturizing effect. Studies have found that it has anti-aging effects. It can promote fibroblast activity, stimulate cell renewal, and promote collagen production. It can even inhibit the damage of AGEs to collagen fibers, so it has a certain anti-glycation effect. It also has a certain promoting effect on the proliferation of stratum corneum cells and a certain effect on strengthening the skin barrier function.

[0063] Hydroxypropyl chitosan: Chitosan is a product of the natural polysaccharide chitin with some acetyl groups removed. It has multiple physiological functions such as biodegradability, biocompatibility, non-toxicity, antibacterial, anti-cancer, lipid-lowering, moisturizing, and immune enhancement. It is widely used in food additives, textiles, agriculture, environmental protection, beauty and health care, cosmetics, antibacterial agents, medical fibers, medical dressings, artificial tissue materials, drug sustained-release materials, gene transduction vectors, biomedical fields, medical absorbable materials, tissue engineering carrier materials, medical and drug development, and other fields and other daily chemical industries. Hydroxypropyl chitosan is one of the derivatives of chitosan. Hydroxypropyl chitosan is introduced into chitosan to make chitosan have better water solubility.

[0064] The skin conditioning agent composed of phytosterol / octyldodecanol lauroyl glutamate, rhamnose and hydroxypropyl chitosan is added to the multifunctional skin cleansing composition. The three cooperate with each other and have a synergistic effect in moisturizing and emollient.

[0065] Preferably, the skin conditioning agent comprises, by weight, any one of 0.1-5 parts of phytosterol / octyldodecanol lauroyl glutamate, 0.1-2 parts of rhamnose or 0.1-2 parts of hydroxypropyl chitosan, or a combination of at least two of them.

[0066] Specific values ​​in the above 0.1-5 parts are, for example, 0.1 part, 0.2 part, 0.4 part, 0.6 part, 0.8 part, 1 part, 1.5 parts, 2 parts, 2.5 parts, 3 parts, 3.5 parts, 4 parts, 4.5 parts, 5 parts, etc.

[0067] Specific numerical values ​​in the above 0.1-2 parts include, for example, 0.1 parts, 0.2 parts, 0.4 parts, 0.6 parts, 0.8 parts, 1 parts, 1.5 parts, 2 parts, etc.

[0068] Preferably, the antioxidant comprises sodium phytate.

[0069] Sodium phytate: Sodium phytate is an organic substance with the chemical formula C6H6Na 12 O 24 P6, in white powder or crystal form, is an important pure natural green additive. Its most notable feature is that it has a strong chelation effect with metal ions, strong antioxidant and color protection. It is an important pure natural green additive. Its most notable feature is that it has a strong chelation effect with metal ions, strong antioxidant and color protection.

[0070] Other antioxidants commonly used in the art may also be used.

[0071] Preferably, the thickener comprises any one of xanthan gum, hydroxyethyl cellulose or carbomer, or a combination of at least two thereof.

[0072] Preferably, the humectant comprises any one or a combination of at least two of erythritol, trehalose, propylene glycol, glycerol, 1,3-butylene glycol, polyethylene glycol, methylpropanediol or sorbitol.

[0073] Preferably, the preservative includes any one of p-hydroxyacetophenone, phenoxyethanol or ethylhexylglycerin, or a combination of at least two thereof.

[0074] In a second aspect, the present invention provides a method for preparing the multifunctional skin cleansing composition containing a biosurfactant as described in the first aspect, the preparation method comprising mixing the surfactant with an antibacterial agent to obtain the composition.

[0075] Preferably, the preparation method comprises: mixing a thickener, water and optional hydroxypropyl chitosan, and then mixing with a surfactant, remaining skin conditioner and optional other raw materials, adjusting the pH of the mixture to 6-7 (e.g. 6, 6.1, 6.2, 6.3, 6.4, 6.5, 6.6, 6.7, 6.8, 6.9, 7, etc.), and then mixing with an antibacterial agent to obtain the product; the other raw materials comprise any one of a moisturizer, a preservative or a fragrance, or a combination of at least two thereof.

[0076] Preferably, the mixing is carried out at 15-40°C, for example, 15°C, 17°C, 20°C, 22°C, 25°C, 27°C, 30°C, 32°C, 35°C, 37°C, 40°C, etc.

[0077] When the skin conditioner includes hydroxypropyl chitosan, during preparation, the hydroxypropyl chitosan is first mixed with a thickener and then other components are added, which can improve the stability of the multifunctional cleansing composition and avoid the precipitation or other instability of hydroxypropyl chitosan or other ingredients.

[0078] Preferably, the preparation method comprises: uniformly mixing hydroxypropyl chitosan, a thickener and water, and then sequentially adding sodium phytate, Sophora japonica resin, rhamnolipid, phytosterol / octyldodecanol lauroyl glutamate, part of cocamidopropyl-PG-dimethylammonium chloride phosphate (the part refers to 10-90% of the formula amount), sodium lauroyl oat amino acids, cocamidopropyl hydroxysulfobetaine, rhamnose, a moisturizer, a preservative, a premixed flavor and the remaining cocamidopropyl-PG-dimethylammonium chloride phosphate, adjusting the pH to 6-7, and finally adding an antibacterial agent and mixing evenly to obtain the product.

[0079] By adopting the above-mentioned adding sequence, the obtained multifunctional cleansing composition has better stability.

[0080] In a third aspect, the present invention provides use of the multifunctional skin cleansing composition containing a biosurfactant as described in the first aspect in the preparation of a cleaning product.

[0081] Preferably, the cleaning product comprises any one of cleansing bubbles, cleansing milk, cleansing cream, shower gel or cleansing soap.

[0082] The numerical range described in the present invention not only includes the point values ​​listed above, but also includes any point values ​​between the above numerical ranges that are not listed. Due to space limitations and for the sake of simplicity, the present invention no longer exhaustively lists the specific point values ​​included in the range.

[0083] Compared with the prior art, the present invention has the following beneficial effects:

[0084] The multifunctional cleansing composition provided by the present invention has dense foam and strong cleaning power when used, is mild and non-irritating, and has the effects of antibacterial, anti-inflammatory, mite removal, anti-oxidation, conditioning and repairing the skin. The raw materials used are easily degradable and conform to the concept of ecological environmental protection. It can be used for facial cleaning, body cleaning, etc.

[0085] The present invention creatively adds a biological surfactant to the multifunctional cleansing composition, and compounds it with a phosphate surfactant, a zwitterionic surfactant and an amino acid surfactant to form a composite surfactant, the mildness, foaming effect and cleaning ability of which are all guaranteed.

[0086] Among them, specific surfactant components: Sophora japonica resin, rhamnolipid, cocamidopropyl-PG-dimethylammonium chloride phosphate, cocamidopropyl hydroxysulfonyl betaine and sodium lauroyl oat amino acids can cooperate with each other and have a synergistic effect in improving the foaming effect and cleaning ability of the product. If they are missing or replaced with similar components, the overall effect will be reduced.

[0087] In addition, the antibacterial agent prepared by using Xanthium sibiricum, hibiscus bark, Atractylodes lancea, Tibetan calamus, Artemisia annua, Inula japonica, grape polyphenols, oleuropein and polylysine as raw materials has good antibacterial and mite removal effects and guaranteed safety. If one component is missing from the raw materials, the antibacterial and mite removal effects of the antibacterial agent obtained by extraction will be reduced to varying degrees, which may be because the raw material components / the extracts of the raw materials play a synergistic role in antibacterial and mite removal. DETAILED DESCRIPTION

[0088] In order to further explain the technical means and effects adopted by the present invention, the technical solution of the present invention is further described below in conjunction with the preferred embodiments of the present invention, but the present invention is not limited to the scope of the embodiments.

[0089] In the following examples, unless otherwise specified, all reagents and consumables were purchased from conventional reagent manufacturers in the field; unless otherwise specified, the experimental methods and technical means used were conventional methods and means in the field.

[0090] Example 1

[0091] This embodiment provides a multifunctional skin cleansing composition containing a biosurfactant, the formula of which is as follows:

[0092] Deionized water balance

[0093] Sodium phytate (Shandong Laiyang Wanjiwei Bioengineering Co., Ltd., model: 99% sodium phytate) 0.01%

[0094] Sophora resin (Nanjing Spike Biotechnology Co., Ltd., model: SL) 10%

[0095] Rhamnolipid (Shaanxi Yikanglong Biotechnology Co., Ltd., model: Rhamnolipid 95%) 1%

[0096] Phytosterol / Octyldodecyl Lauroyl Glutamate (Ajinomoto, model: Eldew PS-203) 1%

[0097] Cocoamidopropyl-PG-dimethylammonium chloride phosphate (Heroda, model: biomimetic phospholipid CPG) 2%

[0098] Sodium lauroyl oat amino acids (France Seppic, model: PROTEOL OAT) 20%

[0099] Cocamidopropyl Hydroxysulfobetaine (Guangzhou Tianci, model: TC-SHD) 3%

[0100] Hydroxypropyl chitosan (Shaanxi Dongshuo Biotechnology Co., Ltd., model: Hydroxypropyl chitosan 98%) 0.1%

[0101] Xanthan gum (also known as xanthan gum, CP Kelco, USA, model: CG-T) 0.5%

[0102] Rhamnose (Chenfeng Biopharmaceuticals (Beijing) Co., Ltd., model: L-rhamnose 98%) 0.1%

[0103] Erythritol 0.1%

[0104] p-Hydroxyacetophenone 0.1%

[0105] Phenoxyethanol 0.1%

[0106] Ethylhexylglycerin 1%

[0107] Water-soluble flavor 0.3%

[0108] Antibacterial compound liquid 1%

[0109] Citric acid (adjust pH to 6.5)

[0110] The preparation method is as follows:

[0111] At room temperature (25°C), hydroxypropyl chitosan and thickener (xanthan gum) were added to deionized water and stirred to dissolve evenly, and then sodium phytate, Sophora japonica resin, rhamnolipid, phytosterol / octyldodecanol lauroyl glutamate, part of cocamidopropyl-PG-dimethyl ammonium chloride phosphate (half of the formula amount), sodium lauroyl oat amino acids, cocamidopropyl hydroxysulfonyl betaine, rhamnose, moisturizer (erythritol), preservatives (p-hydroxyacetophenone, phenoxyethanol, ethylhexylglycerin), pre-mixed water-soluble flavors and the remaining cocamidopropyl-PG-dimethyl ammonium chloride phosphate were added in sequence, and citric acid was added and stirred evenly, and the pH was adjusted to 6.5; finally, the antibacterial composite solution was added and stirred evenly.

[0112] Wherein, the preparation method of the antibacterial composite liquid is:

[0113] After crushing the fruit of Xanthium sibiricum, the bark of hibiscus, Atractylodes lancea, Tibetan calamus, Artemisia annua, and Inula odorata, 1% of the fruit of Xanthium sibiricum, 1% of the bark of hibiscus, 1% of the Atractylodes lancea, 1% of the Tibetan calamus, 1% of the Artemisia annua, and 1% of the Inula odorata were added to a 50% (volume fraction) ethanol aqueous solution (remainder) by mass fraction, mixed and stirred evenly, soaked at room temperature (20° C.) for 70 hours, filtered, the filtrate was collected, concentrated under reduced pressure to a solution volume of 1 / 10 of the filtrate, and sterilized. Taking the mass of the concentrated solution as 100%, 0.1% of grape polyphenols (Shaanxi Mufei Biotechnology Co., Ltd., grape skin anthocyanins 25%), 0.1% of oleuropein (Shaanxi Haochen Biotechnology Co., Ltd., 70% oleuropein), and 1% of polylysine (Guangzhou Hongyi Food Additive Co., Ltd., cosmetic grade polylysine) were added thereto, stirred and dissolved evenly, and the antibacterial composite liquid was obtained.

[0114] Example 2

[0115] This embodiment provides a multifunctional skin cleansing composition containing a biosurfactant, the formula of which is as follows:

[0116] Deionized water balance

[0117] Sodium Phytate 0.1%

[0118] Sophora resin 1%

[0119] Rhamnolipid 10%

[0120] Phytosteryl / Octyldodecyl Lauroyl Glutamate 0.1%

[0121] Cocamidopropyl-PG-dimonium chloride phosphate 0.5%

[0122] Sodium Lauroyl Oat Amino Acids 30%

[0123] Cocamidopropyl Hydroxysultaine 1%

[0124] Hydroxypropyl chitosan 0.5%

[0125] Hydroxyethylcellulose 0.25%

[0126] Rhamnose 0.1%

[0127] Trehalose 2%

[0128] p-Hydroxyacetophenone 0.1%

[0129] Phenoxyethanol 1%

[0130] Ethylhexylglycerin 0.05%

[0131] Water-soluble flavor 0.1%

[0132] Citric acid (adjust pH to 6.2)

[0133] Antibacterial compound liquid 7%

[0134] The preparation method thereof is as described in Example 1.

[0135] Wherein, the preparation method of the antibacterial composite liquid is:

[0136] After crushing the fruit of Xanthium sibiricum, the bark of hibiscus, Atractylodes lancea, Tibetan calamus, Artemisia annua, and Inula odorata, 0.1% of the fruit of Xanthium sibiricum, 2% of the bark of hibiscus, 0.2% of the Atractylodes lancea, 0.1% of the Tibetan calamus, 3% of the Artemisia annua, and 0.5% of the Inula odorata are added to a 40% (volume fraction) ethanol aqueous solution (the remainder) by mass fraction, mixed and stirred evenly, soaked at room temperature for 50 hours, filtered, the filtrate is collected, concentrated under reduced pressure to a volume of 1 / 2 of the filtrate, and sterilized. Based on the mass of the concentrated solution being 100%, 0.5% of grape polyphenols, 0.5% of oleuropein, and 10% of polylysine are added thereto, stirred and dissolved evenly, and the antibacterial composite liquid is obtained.

[0137] Example 3

[0138] This embodiment provides a multifunctional skin cleansing composition containing a biosurfactant, the formula of which is as follows:

[0139] Deionized water balance

[0140] Sodium Phytate 0.2%

[0141] Sophora resin 5%

[0142] Rhamnolipid 5%

[0143] Phytosteryl / Octyldodecyl Lauroyl Glutamate 5%

[0144] Cocamidopropyl-PG-dimonium chloride phosphate 3%

[0145] Sodium Lauroyl Oat Amino Acids 10%

[0146] Cocamidopropyl Hydroxysultaine 2%

[0147] Hydroxypropyl Chitosan 2%

[0148] SF-1 thickener 0.1%

[0149] Rhamnose 2%

[0150] Propylene glycol 0.5%

[0151] p-Hydroxyacetophenone 0.5%

[0152] Phenoxyethanol 0.1%

[0153] Ethylhexylglycerin 0.5%

[0154] Water-soluble flavor 0.5%

[0155] Citric acid (adjust pH to 6.8)

[0156] Antibacterial compound liquid 5%

[0157] The preparation method thereof is as described in Example 1.

[0158] Wherein, the preparation method of the antibacterial composite liquid is:

[0159] After crushing the fruit of Xanthium sibiricum, the bark of Hibiscus syringae, Atractylodes lancea, Tibetan calamus, Artemisia annua, and Inula odorifera, 3% of the fruit of Xanthium sibiricum, 1% of the bark of Hibiscus syringae, 0.5% of the Atractylodes lancea, 0.2% of the Tibetan calamus, 0.1% of the Artemisia annua, and 1.2% of the Inula odorifera are added to a 60% (volume fraction) ethanol aqueous solution (the remainder) by mass fraction, mixed and stirred evenly, soaked at room temperature for 90 hours, filtered, the filtrate is collected, concentrated under reduced pressure until the volume of the solution is 1 / 2 of the filtrate, and sterilized. Based on the mass of the concentrated solution being 100%, 1% of grape polyphenols, 0.5% of oleuropein, and 5% of polylysine are added thereto, stirred and dissolved evenly, and the antibacterial composite liquid is obtained.

[0160] Example 4

[0161] This embodiment provides a multifunctional skin cleansing composition containing a biosurfactant, the formula of which differs from that of Example 1 only in that the antibacterial agent does not contain "Xanthium sibiricum extract". Specifically, in the preparation of the antibacterial composite liquid, Xanthium sibiricum is not added, and the missing amount is made up in proportion with hibiscus bark, atractylodes, Tibetan calamus, artemisia annua and inula japonica, while other components and their contents remain unchanged. The specific preparation method refers to Example 1.

[0162] Example 5

[0163] This embodiment provides a multifunctional skin cleansing composition containing a biosurfactant, the formula of which differs from that of Example 1 only in that the antibacterial agent does not contain "hibiscus bark extract". Specifically, in the preparation of the antibacterial composite liquid, hibiscus bark is not added, and the missing amount is made up in proportion with Xanthium sibiricum, Atractylodes lancea, Tibetan calamus, Artemisia annua and Inula japonica, while other components and their contents remain unchanged. The specific preparation method refers to Example 1.

[0164] Example 6

[0165] This embodiment provides a multifunctional skin cleansing composition containing a biosurfactant, the formula of which differs from that of Example 1 only in that the antibacterial agent does not contain "Atractylodes lancea extract". Specifically, in the preparation of the antibacterial composite liquid, Atractylodes lancea is not added, and the missing amount is made up in proportion with Xanthium sibiricum, Hibiscus sylvestris bark, Tibetan calamus, Artemisia annua and Inula japonica, while other components and their contents remain unchanged. The specific preparation method refers to Example 1.

[0166] Example 7

[0167] This embodiment provides a multifunctional skin cleansing composition containing a biosurfactant, the formula of which differs from that of Example 1 only in that the antibacterial agent does not contain "Tibetan calamus extract". Specifically, in the preparation of the antibacterial composite liquid, Tibetan calamus is not added, and the missing amount is made up in proportion with Xanthium sibiricum, Hibiscus sylvestris bark, Atractylodes lancea, Artemisia annua and Inula japonica, while other components and their contents remain unchanged. The specific preparation method refers to Example 1.

[0168] Example 8

[0169] This embodiment provides a multifunctional skin cleansing composition containing a biosurfactant, the formula of which differs from that of Example 1 only in that the antibacterial agent does not contain "Artemisia annua extract". Specifically, in the preparation of the antibacterial composite liquid, Artemisia annua is not added, and the missing amount is made up in proportion with Xanthium sibiricum, Hibiscus sylvestris bark, Atractylodes macrocephala, Tibetan calamus and Inula japonica, while other components and their contents remain unchanged. The specific preparation method refers to Example 1.

[0170] Example 9

[0171] This embodiment provides a multifunctional skin cleansing composition containing a biosurfactant, the formula of which differs from that of Example 1 only in that the antibacterial agent does not contain "Inula extract". Specifically, in the preparation of the antibacterial composite liquid, Inula is not added, and the missing amount is made up in proportion with Xanthium sibiricum, Hibiscus sylvestris bark, Atractylodes macrocephala, Tibetan calamus and Artemisia annua, while other components and their contents remain unchanged. The specific preparation method refers to Example 1.

[0172] Example 10

[0173] This embodiment provides a multifunctional skin cleansing composition containing a biosurfactant, the formula of which differs from that of Example 1 only in that the antibacterial agent does not contain "grape polyphenols". Specifically, in the preparation of the antibacterial composite liquid, grape polyphenols are not added, and the missing amount is supplemented with oleuropein and polylysine in proportion, while other components and contents remain unchanged. The specific preparation method refers to Example 1.

[0174] Embodiment 11

[0175] This embodiment provides a multifunctional skin cleansing composition containing a biosurfactant, the formula of which differs from that of Example 1 only in that the antibacterial agent does not contain "oleuropein". Specifically, in the preparation of the antibacterial composite liquid, oleuropein is not added, and the missing amount is made up with grape polyphenols and polylysine in proportion, while other components and contents remain unchanged. The specific preparation method refers to Example 1.

[0176] Example 12

[0177] This embodiment provides a multifunctional skin cleansing composition containing a biosurfactant, the formula of which differs from that of Example 1 only in that the antibacterial agent does not contain "polylysine". Specifically, in the preparation of the antibacterial composite liquid, polylysine is not added, and the missing amount is made up with grape polyphenols and oleuropein in proportion, while other components and contents remain unchanged. The specific preparation method refers to Example 1.

[0178] Example 13

[0179] The present embodiment provides a multifunctional skin cleansing composition containing a biosurfactant, the formula of which differs from that of Example 1 only in that an equal amount of "sodium lauroyl oat amino acids" in the surfactant is replaced with "a combination of sodium lauryl glutamate and sodium cocoyl aminopropionate (mass ratio of 1:1)", and other components and contents remain unchanged. The preparation method refers to Example 1.

[0180] Comparative Example 1

[0181] This comparative example provides a multifunctional skin cleansing composition containing a biosurfactant. The formula thereof is different from that of Example 1 only in that "rhamnolipid" is missing from the surfactant and the missing amount is replaced by Sophora japonica resin. The other components and contents remain unchanged. The preparation method is similar to that of Example 1.

[0182] Comparative Example 2

[0183] This comparative example provides a multifunctional skin cleansing composition containing a biosurfactant. The formula thereof is different from that of Example 1 only in that the surfactant lacks "sophora resin" and the missing amount is replaced by rhamnolipid, and the other components and contents remain unchanged. The preparation method is referred to Example 1.

[0184] Comparative Example 3

[0185] This comparative example provides a multifunctional skin cleansing composition containing a biosurfactant, the formula of which differs from that of Example 1 only in that "cocamidopropyl-PG-dimethylammonium chloride phosphate" is missing from the surfactant, and the missing amount is made up with other surfactant components in proportion, while other components and contents remain unchanged. The preparation method refers to Example 1.

[0186] Comparative Example 4

[0187] This comparative example provides a multifunctional skin cleansing composition containing a biosurfactant. The formula thereof is different from that of Example 1 only in that "cocamidopropyl hydroxysulfonyl betaine" is missing from the surfactant, and the missing amount is made up with other surfactant components in proportion, while other components and contents remain unchanged. The preparation method refers to Example 1.

[0188] Effect test

[0189] Test Example 1- Foaming Effect Test

[0190] The foaming ability of the multifunctional cleansing compositions prepared in Examples 1-3, Example 13 and Comparative Examples 1-4 was evaluated using a Roche foam tester (and a commercially available cleansing foam was used as a control). First, the super constant temperature water bath was started to reach 40°C, and the temperature of the water bath in the tube jacket was also kept consistent: 2.5 g of sample was weighed into a 1 L volumetric flask, 150 ppm hard water (MgSO4.7H2O 0.148 g and CaCl2 0.0999g in a 1L volumetric flask, dilute with distilled water to the scale), shake it gently, and heat it to 40℃ for measurement: rinse the inner wall of the graduated tube with distilled water and then with the test solution, close the graduated tube piston, inject 50mL of test solution with another dropper, let it stand for 1min, adjust the piston so that the liquid level is just at the 50mL scale: fill the dropper with 200mL of test solution, place the dropper on a prepared tube rack and make it perpendicular to the cross section of the graduated tube, so that the solution flows to the center of the graduated tube: open the dropper piston to allow the solution to flow down, when the solution has flowed out, start the stopwatch, and record the height of the foam after 5min (each group of tests was repeated three times and the average value was used for statistics). The results are shown in Table 1.

[0191] Table 1

[0192]

[0193]

[0194] Test Example 2-Antibacterial Test

[0195] (1) Preparation of the sample to be tested: Select filter paper with strong water absorption and uniform texture, use a hole puncher to make a 6 mm diameter round paper sheet, and dry and sterilize at 120°C for 2 hours. Add each paper sheet into the test sample (composition of Examples 1-12) diluted ten times, make the paper sheet evenly soaked, put it in a sterile plate, dry it in a 37°C oven, divide it into small bottles, seal it, and store it at 4°C for use:

[0196] (2) Use a sterile cotton swab to dip into the freshly prepared diluted bacterial solution (Staphylococcus aureus and Propionibacterium acnes), squeeze out the excess bacterial solution on the tube wall, evenly spread it on the NA plate culture medium, sweep around the edge of the plate, cover it, and dry it for 2-3 minutes. Use tweezers to take the above-mentioned dry drug-containing filter paper and stick it in the center of the plate. Make three parallel groups for each model strain, and take the average value of the inhibition zone. The above operations are carried out in a biological safety cabinet: Among them, Staphylococcus aureus is cultured at a constant temperature of 37°C for 18 hours, and Propionibacterium acnes is cultured at a constant temperature of 37°C for 72 hours. The size of each inhibition zone is observed and measured: The results are shown in Table 2:

[0197] Table 2

[0198]

[0199]

[0200] Test Example 3 - Mite Removal Test

[0201] (1) Obtaining human Demodex mites: Demodex mites were obtained by the transparent tape sticking method. The specific steps were as follows: the subjects were asked to wash their faces before going to sleep, and then stick transparent tapes 6 cm long and 1.5 cm wide on the forehead, cheeks, nose, chin and other parts. The next morning, the tapes were peeled off and stuck to the parts of the body. The samples were examined under a microscope and sealed on a glass slide.

[0202] (2) Test grouping: 0.1 mg / cm2 of the sample to be tested (composition of Examples 1-12) was dripped onto each glass slide, and the sample to be tested was spread evenly to ensure full contact with the insect body. The sample was placed in an environment with a relative humidity of 70-80% and a temperature of 26-28°C. After 24 hours, the sample was taken out and the survival of the Demodex mite was observed under a microscope. The sample was observed continuously for 3 minutes under a microscope at 400×. The sample was considered dead if the whole limb or paw of the insect body was not moving. The results are shown in Table 3:

[0203] Table 3

[0204]

[0205]

[0206] Test Example 4-Irritation Test

[0207] The irritation of the products of the above embodiments and comparative examples was tested by using the red blood cell hemolysis experiment.

[0208] Test principle: The red blood cell hemolysis test is based on the principle that the lipids and proteins on the surface of the skin are similar to the cell membrane of red blood cells. The degree of irritation is evaluated by detecting the effect of the test sample on the hemoglobin release and hemoglobin denaturation of red blood cells.

[0209] Preparation of sample suspension

[0210] Normal saline or PBS is generally used as the solvent. For samples that are insoluble in saline or PBS, DMSO or ethanol can be used as the solvent. It is necessary to ensure that each diluted sample suspension is fully mixed. Sample concentration range detection experiment: The test substance is prepared into a series of gradient suspensions such as 0.001%, 0.01%, 0.1%, 1%, 10%, 20%, 30%, 40%, etc. with the solvent, mixed with the red blood cell suspension in an equal volume ratio, incubated at 37°C for 3 hours, centrifuged and the supernatant was taken. The absorbance was measured at 410nm, 540nm, and 575nm on an ELISA instrument, and compared with the absorbance values ​​of the negative control and positive control. According to the results, a suitable concentration is selected in the upper and lower range covering the hemolysis rate of 50% as the formal test concentration range of the sample.

[0211] According to the results of the detection experiment, select the appropriate test concentration and prepare a series of sample concentrations.

[0212] Hemolysis and protein denaturation determination

[0213] In a 2 mL centrifuge tube, add 500 μL of the test substance at different concentrations and 500 μL of 2% sheep red blood cell suspension (mix in equal volumes), and mix well. Set 3 parallels for each concentration. Use the solvent (normal saline or PBS) as the negative control (i.e., no hemolysis at all), and use 0.1% SDS solution as the positive control (i.e., complete hemolysis). After incubating in a 37 °C constant temperature incubator for 3 h, centrifuge at 2000 rpm / min for 3 min, pipette 10 μL of the supernatant and add it to the microplate (previously add 90 μL of normal saline or PBS to each well), mix well, and measure the absorbance at 410 nm, 540 nm, and 575 nm with a microplate reader.

[0214] Calculate the hemolysis rate (%) according to the following formula:

[0215] Hemolysis rate (%) = (A - C) / (B - C) × 100;

[0216] In the formula, A is the average absorbance of each sample concentration at 410 nm, B is the average absorbance of the positive control at 410 nm, and C is the average absorbance of the negative control at 410 nm.

[0217] Determine the concentration of the test substance HC50 (mg / L) that causes 50% hemolysis of red blood cells by the regression equation.

[0218] Calculate the protein denaturation index DI (%) according to the following formula:

[0219] DI (%) = (A575nm / A540nm of the test substance) / (A575nm / A540nm of the positive control) × 100.

[0220] Calculate the L / D value of the sample according to the HC50 value and the DI value: L / D = HC50 / DI; According to the RBC experiment classification standard of the European Centre for the Validation of Alternative Methods (ECVAM), classify the irritation degree of cosmetics. The classification method is as follows:

[0221] L / D > 100 is evaluated as non-irritating;

[0222] 10 < L / D ≤ 100 is evaluated as slightly irritating;

[0223] 1 < L / D ≤ 10 is evaluated as mildly irritating;

[0224] 0.1 < L / D ≤ 1 is evaluated as moderately irritating;

[0225] L / D ≤ 0.1 is evaluated as severely irritating.

[0226] The test results are shown in Table 4:

[0227] Table 4

[0228] Group L / D evaluate Example 1 L / D>100 Non-irritating Example 2 L / D>100 Non-irritating Example 3 L / D>100 Non-irritating Example 4 L / D>100 Non-irritating Example 5 L / D>100 Non-irritating Example 6 L / D>100 Non-irritating Example 7 L / D>100 Non-irritating Example 8 L / D>100 Non-irritating Example 9 L / D>100 Non-irritating Example 10 L / D>100 Non-irritating Embodiment 11 L / D>100 Non-irritating Example 12 L / D>100 Non-irritating Example 13 L / D>100 Non-irritating Comparative Example 1 L / D>100 Non-irritating Comparative Example 2 L / D>100 Non-irritating Comparative Example 3 L / D>100 Non-irritating Comparative Example 4 L / D>100 Non-irritating

[0229] The results show that the products of each embodiment and comparative example are non-irritating, safe, and can be used for human testing.

[0230] Test Example 5 - Crowd Test (Cleaning Effect, Foam Density, Mildness, Emollient)

[0231] 180 volunteers with oily skin were selected, regardless of gender, and divided into random groups, with 10 people in each group.

[0232] Samples to be tested: products of Examples 1-13 and Comparative Examples 1-4, and a commercially available facial cleansing bubble.

[0233] A fixed part of the cheek of the test subject was used as the test area. The CBS skin analysis system was used to measure the skin oil content of each test subject before cleansing and the oil content of each group of samples after cleansing for the same time using an equal amount at room temperature. The oil removal rate was calculated according to the following formula: Oil removal rate = (skin oil content before cleansing - skin oil content after cleansing) / skin oil content before cleansing × 100%. The cleansing effect of the product on the skin was investigated. The measurement results (average value) are shown in Table 5.

[0234] At the same time, each subject was asked to rate the foam density, mildness and emollient properties of the product during cleansing, with a full score of 10. If the foam was dense, mild (no irritation during use) and emollient (skin was not tight after cleansing), 8-10 points were given; if the foam was dense, mild and emollient was average, 5-7 points were given; if the foam was dense, mild and emollient was poor, 1-4 points were given. The scores were all integers, and the average scores of the 10 people in each group were calculated and listed in Table 5.

[0235] Table 5

[0236]

[0237]

[0238] In summary, the composition provided by the present invention has dense foam and strong cleaning power when used, is mild and non-irritating, and has the effects of antibacterial, anti-inflammatory, mite removal, anti-oxidation, conditioning and repairing the skin.

[0239] From the comparison results between Example 1, Example 13, and Comparative Examples 1-4, it can be seen that the specific surfactant components: Sophora resin, rhamnolipid, cocamidopropyl-PG-dimethylammonium chloride phosphate, cocamidopropyl hydroxysulfobetaine and sodium lauroyl oat amino acids can cooperate with each other and have a synergistic effect in improving the foaming effect and cleaning ability of the product. If they are missing or replaced with similar components, the comprehensive effect will be reduced.

[0240] From the comparison results of Example 1 and Examples 4-12, it can be seen that the antibacterial agent prepared by using Xanthium sibiricum, Hibiscus bark, Atractylodes lancea, Tibetan calamus, Artemisia annua, Inula japonica, grape polyphenols, oleuropein and polylysine as raw materials has good antibacterial and mite removal effects and guaranteed safety. If one component is missing from the raw materials, the antibacterial and mite removal effects of the antibacterial agent obtained by extraction will be reduced to varying degrees, which may be because the raw material components / the extracts of the raw materials play a synergistic role in antibacterial and mite removal.

[0241] The applicant declares that the present invention uses the above-mentioned embodiments to illustrate a multifunctional skin cleansing composition containing a biosurfactant and its preparation method and application, but the present invention is not limited to the above-mentioned embodiments, that is, it does not mean that the present invention must rely on the above-mentioned embodiments to be implemented. Those skilled in the art should understand that any improvement of the present invention, equivalent replacement of various raw materials of the product of the present invention, addition of auxiliary components, selection of specific methods, etc., all fall within the protection scope and disclosure scope of the present invention.

[0242] The preferred embodiments of the present invention are described in detail above. However, the present invention is not limited to the specific details in the above embodiments. Within the technical concept of the present invention, a variety of simple modifications can be made to the technical solution of the present invention, and these simple modifications all belong to the protection scope of the present invention.

[0243] It should also be noted that the various specific technical features described in the above specific embodiments can be combined in any suitable manner without contradiction. In order to avoid unnecessary repetition, the present invention will not further describe various possible combinations.

Claims

1. A multifunctional skin cleansing composition containing a biosurfactant, characterized in that: The multifunctional skin cleansing composition comprises a surfactant and an antibacterial agent, wherein the surfactant comprises a biosurfactant, a phosphate surfactant, a zwitterionic surfactant and an amino acid surfactant: In parts by weight, the biosurfactant includes 1-10 parts of Sophora resin and 1-10 parts of rhamnolipid, the phosphate surfactant includes 0.5-5 parts of cocamidopropyl-PG-dimethylammonium chloride phosphate, the zwitterionic surfactant includes 1-5 parts of cocamidopropyl hydroxysulfonyl betaine, and the amino acid surfactant includes 10-30 parts of sodium lauroyl oat amino acids; The antibacterial agent, in an amount of 0.1-20 parts, comprises a combination of Xanthium sibiricum extract, Hibiscus sylvestris bark extract, Atractylodes macrocephala extract, Acorus calamus extract, Artemisia annua extract, Inula japonica extract, grape polyphenols, oleuropein and polylysine.

2. The multifunctional skin cleansing composition containing a biosurfactant according to claim 1, characterized in that: The antibacterial agent is prepared by a preparation method comprising the following steps: mixing Xanthium sibiricum, hibiscus bark, Atractylodes lancea, Tibetan calamus, Artemisia annua, and Inula japonica with an extraction solvent to obtain a mixed solution, extracting, filtering, collecting the filtrate, and mixing it with grape polyphenols, oleuropein, and polylysine to obtain the antibacterial agent.

3. The multifunctional skin cleansing composition containing a biosurfactant according to claim 2, characterized in that: The extraction solvent includes an ethanol aqueous solution with a volume concentration of 40-60%.

4. The multifunctional skin cleansing composition containing a biosurfactant according to claim 2, characterized in that: The components in the mixed solution are calculated by mass percentage as follows: 0.1%-3% of Xanthium sibiricum fruit, 0.1%-3% of hibiscus bark, 0.1%-3% of Atractylodes lancea, 0.1%-3% of Tibetan calamus, 0.1%-3% of Artemisia annua, 0.1%-3% of Inula japonica, and the remainder is extraction reagent.

5. The multifunctional skin cleansing composition containing a biosurfactant according to claim 2, characterized in that: The extraction method includes immersion extraction.

6. The multifunctional skin cleansing composition containing a biosurfactant according to claim 2, characterized in that: The extraction is carried out at 15-40° C. and the extraction time is 50-90 h.

7. The multifunctional skin cleansing composition containing a biosurfactant according to claim 2, characterized in that: After collecting the filtrate, the process further comprises concentrating the filtrate, and the volume of the obtained concentrated liquid is 1 / 10-1 / 2 of the volume of the filtrate.

8. The multifunctional skin cleansing composition containing a biosurfactant according to claim 7, characterized in that: Taking the mass of the filtrate or the concentrated solution as 100%, the mass of the grape polyphenol is 0.1%-1%, the mass of oleuropein is 0.1%-1%, and the mass of polylysine is 1%-10%.

9. The multifunctional skin cleansing composition containing a biosurfactant according to claim 7, characterized in that: The concentration method includes concentration under reduced pressure.

10. The multifunctional skin cleansing composition containing a biosurfactant according to claim 1, characterized in that: The multifunctional skin cleansing composition further comprises any one or a combination of at least two of a skin conditioner, an antioxidant, a thickener, a moisturizer, a preservative or a fragrance.

11. The multifunctional skin cleansing composition containing a biosurfactant according to claim 10, characterized in that: The multifunctional cleansing composition further comprises, by weight, any one of 0.1-10 parts of skin conditioner, 0.01-0.2 parts of antioxidant, 0.1-0.5 parts of thickener, 0.1-5 parts of moisturizer, 0.2-3 parts of preservative or 0.1-0.5 parts of fragrance, or a combination of at least two thereof.

12. The multifunctional skin cleansing composition containing a biosurfactant according to claim 10, characterized in that: The skin conditioning agent comprises any one of phytosterol / octyldodecanol lauroyl glutamate, rhamnose or hydroxypropyl chitosan or a combination of at least two thereof.

13. The multifunctional skin cleansing composition containing a biosurfactant according to claim 12, characterized in that: The skin conditioning agent comprises, by weight, any one of 0.1-5 parts of phytosterol / octyldodecanol lauroyl glutamate, 0.1-2 parts of rhamnose or 0.1-2 parts of hydroxypropyl chitosan, or a combination of at least two of them.

14. The multifunctional skin cleansing composition containing a biosurfactant according to claim 10, characterized in that: The antioxidant includes sodium phytate.

15. The multifunctional skin cleansing composition containing a biosurfactant according to claim 10, characterized in that: The thickener includes any one of xanthan gum, hydroxyethyl cellulose or carbomer, or a combination of at least two of them.

16. The multifunctional skin cleansing composition containing a biosurfactant according to claim 10, characterized in that: The moisturizing agent includes any one of erythritol, trehalose, propylene glycol, glycerin, 1,3-butylene glycol, polyethylene glycol, methylpropanediol or sorbitol, or a combination of at least two thereof.

17. The multifunctional skin cleansing composition containing a biosurfactant according to claim 10, characterized in that: The preservative includes any one of p-hydroxyacetophenone, phenoxyethanol or ethylhexylglycerin, or a combination of at least two thereof.

18. The method for preparing a multifunctional skin cleansing composition containing a biosurfactant according to any one of claims 1 to 9, characterized in that: The preparation method comprises mixing a surfactant and an antibacterial agent to obtain the product.

19. The preparation method according to claim 18, characterized in that: The preparation method comprises: mixing a thickener, water and optional hydroxypropyl chitosan, and then mixing with a surfactant, remaining skin conditioning agent and other optional raw materials, adjusting the pH of the mixed solution to 6-7, and then mixing with an antibacterial agent to obtain; The other raw materials include any one of moisturizers, preservatives or fragrances, or a combination of at least two of them.

20. The preparation method according to claim 18, characterized in that: The mixing is carried out at 15-40°C.

21. Use of the multifunctional skin cleansing composition containing a biosurfactant according to any one of claims 1 to 17 in the preparation of a cleaning product.

22. The use according to claim 21, characterized in that The cleaning product comprises any one of cleansing bubbles, cleansing milk, cleansing cream, shower gel or cleansing soap.

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