A system and method for section transfer of a frozen section

Abstract

The application discloses a kind of frozen section collection transfer system and collection transfer method, and collection transfer system includes unwinding roller, pressing piece roller, winding roller and activity winding tape on unwinding roller, pressing piece roller, winding roller in sequence, it is provided with heat preservation channel between pressing piece roller and winding roller, it is provided with refrigeration device in heat preservation channel, transfer port is opened in heat preservation channel side wall, and it is provided with the drive assembly for clamping slide glass outside transfer port, slide glass is parallel with the tape at transfer port, and drive assembly drives slide glass to enter and exit transfer port.Gelatin band is collected after cutting after the frozen section in pressing piece roller, then in the transportation process to winding roller, low-temperature environment in transportation process is realized using heat preservation channel and refrigeration device, to avoid frozen section melting and adhering on gelatin band, slide glass enters and exits heat preservation channel by transfer port, so that frozen section to slide glass can be transferred inside heat preservation channel.

Description

TECHNICAL FIELD

[0001] The present application belongs to the field of biomedicine, and more particularly relates to a system and method for collecting and transferring frozen sections. BACKGROUND

[0002] In the field of biomedicine, it is often necessary to use mechanical cutting to make biological tissues into thin sections of tens and microns, and to collect these sections for microscopic imaging or biochemical analysis. Currently, there are two schemes for collecting tissue sections for push-type cutting, one is a circulating collection method based on a transmission belt, and the other is a roll-to-roll collection method based on an adhesive tape.

[0003] Among them, the transmission belt type section collecting method, the transmission belt continuously passes through the water immersion, increases the surface tension of the transmission belt, and completes the collection of sample sections. However, the existing transmission belt type section collecting scheme is limited by the thickness of the transmission belt, and cannot achieve precise micro-contact adhesion of the transmission belt and the knife, but can only rely on the connection between the sections formed by continuous cutting for uninterrupted collection. In addition, the transmission belt is recycled, and the tissue adhered to the transmission belt will contaminate the subsequent sections.

[0004] The roll-to-roll section collecting method based on the adhesive tape is to complete the sample collection by pasting through the cooperation of the winding roller and the unwinding roller, combined with the adhesion of the adhesive tape, and the method of cutting and collecting synchronously. In the cutting process, the tight adhesive tape can provide support and protection for the tissue sections, and to some extent improve the quality of section collection.

[0005] However, no matter which of the above section collecting methods, the entire section collecting loop is exposed to the air at room temperature. For frozen samples, the cutting environment is usually -10℃ to -25℃, and the section collecting loop environment is at room temperature. Therefore, the frozen sections after cutting will be firmly adhered to the adhesive tape after slight melting, which not only affects the quality of the frozen sections, but also makes it difficult to completely transfer them from the adhesive tape. SUMMARY

[0006] In view of the above defects or improvement needs of the prior art, the present application provides a section collecting and transferring system and method for frozen sections, which aims to solve the problems of heat preservation and transfer in the existing frozen section collecting process.

[0007] To achieve the above object, according to one aspect of the present application, a system for transferring frozen sections is provided, which comprises a unwinding roller, a pressing roller, a winding roller, a movable adhesive tape wound around the unwinding roller, the pressing roller and the winding roller, a heat preservation channel arranged between the pressing roller and the winding roller, a refrigeration device arranged in the heat preservation channel, a transfer port formed in the side wall of the heat preservation channel, and a driving assembly arranged outside the transfer port for clamping a glass slide, wherein the glass slide is parallel to the adhesive tape at the transfer port, and the driving assembly drives the glass slide to enter or exit the transfer port.

[0008] When the frozen sections are collected by the adhesive tape at room temperature, the frozen sections will be firmly adhered to the adhesive tape due to melting, and then the adhesive tape and the frozen sections are soaked in chemical reagents during biochemical operations such as immunohistochemical staining, the chemical reagents will also react with the adhesive tape, which will affect the subsequent analysis results. In addition, during laser microdissection or sampling with a sampling needle, the adhesive tape will be cut and captured together, and the adhesive tape will be introduced as impurities, which will affect the purity of the biological tissue. The present application not only solves the problem of heat preservation of the frozen sections during the collection of the frozen sections, but also considers the problem of transferring the frozen sections from the adhesive tape to the glass slide. Through the above technical scheme, the adhesive tape collects the cut frozen sections at the pressing roller, and then uses the heat preservation channel and the refrigeration device to realize a low-temperature environment during the transportation process to the winding roller, so as to avoid the melting of the frozen sections and affect the imaging observation, and also to avoid the firm adhesion of the frozen sections to the adhesive tape due to the temperature difference between the frozen sections and the adhesive tape. At the same time, the transfer port is formed in the side wall of the heat preservation channel, and the glass slide enters or exits the heat preservation channel through the transfer port, so that the transfer of the frozen sections to the glass slide can be completed in the heat preservation channel.

[0009] Further, the heat preservation channel comprises a heat conduction pipe and a heat preservation layer covering the heat conduction pipe, and the refrigeration device is located between the heat conduction pipe and the heat preservation layer and is fixed on the outer wall of the heat conduction pipe.

[0010] Further, the heat conduction pipe comprises a plurality of pipe sections, and adjacent two pipe sections are detachably connected, and the cross section of the pipe section is in the shape of a Chinese character 'Kou'.

[0011] Further, the pipe section is made of high-thermal-conductivity material, the heat preservation layer is made of low-thermal-conductivity material, and the connection part of adjacent two pipe sections is filled with high-thermal-conductivity material.

[0012] Further, an expansion material is pasted in the heat preservation channel, and the expansion material is located on the side of the adhesive tape away from the transfer port.

[0013] Further, the two ends of the heat conduction pipe are provided with adjustable openings.

[0014] Further, the heat conduction pipe is provided with a gate at the opening of both ends, and the gate is fixed on a one-dimensional moving platform.

[0015] Further, the heat conduction pipe is provided with a gate at the opening of both ends, and the gate is fixed on a one-dimensional moving platform.

[0016] The application also provides a method for transferring a frozen section, which is realized based on the aforementioned section transferring system and comprises the following steps.

[0017] The winding roller starts winding the adhesive tape, the adhesive tape is pasted with the frozen section at the sectioning roller, and the slide glass is clamped in the driving device; when the frozen section on the adhesive tape moves to the transferring port in the heat preservation channel, the driving device drives the slide glass into the transferring port;

[0018] The winding roller stops winding the adhesive tape, the frozen section is pasted with the slide glass, the adsorption force of the slide glass to the frozen section is greater than the adsorption force of the adhesive tape to the frozen section, and the biological section is smoothly transferred to the slide glass.

[0019] Further, before the adhesive tape starts pasting the frozen section, the opening size of both ends of the heat conduction pipe is adjusted, specifically: first, completely close the gate and open the white light LED lamp, press the adhesive tape in the heat conduction pipe, then drive the gate to gradually open through the one-dimensional moving platform, the step distance is 0.1 μm, and the color of the transmitted light between the gate and the adhesive tape is observed with the naked eye; when the color is red, the one-dimensional moving platform stops moving, and the opening size of both ends of the heat conduction pipe is determined. BRIEF DESCRIPTION OF DRAWINGS

[0020] Figure 1 is a schematic diagram of the overall structure of the section transferring system Figure 1 ;

[0021] Figure 2 is a schematic diagram of the structure of the heat preservation channel;

[0022] Figure 3 is a schematic diagram of the cross-sectional structure of the heat preservation channel;

[0023] Figure 4 is a schematic diagram of the structure of the connection between adjacent two pipe sections;

[0024] Figure 5 is a schematic diagram of the overall structure of the section transferring system Figure 2 ;

[0025] Figure 6 is a schematic diagram of the transfer of the frozen section from the adhesive tape to the slide glass in the cross section of the heat conduction pipe;

[0026] Figure 7 is a schematic diagram of the state change of the expanded material when the frozen section is smoothly transferred.

[0027] In the figure, 1, unwinding roller; 2, pressing roller; 3, winding roller; 4, adhesive tape; 5, heat preservation channel; 6, refrigeration device; 7, heat conduction pipe; 8, heat preservation layer; 9, pipe; 10, copper adhesive tape; 11, gate; 12, transfer port; 13, expanded material; 14, linear motor; 15, clamp; 16, electrostatic generator; 17, high-thermal-conductivity material. DETAILED DESCRIPTION

[0028] In order to make the objects, technical solutions and advantages of the present application clearer, the present application will be further described in detail below with reference to the drawings and embodiments. It should be understood that the specific embodiments described herein are only used to explain the present application and not used to limit the present application. In addition, the technical features involved in the various embodiments of the present application described below can be combined with each other as long as they do not conflict with each other.

[0029] As shown in Figure 1 and Figure 2 , the present application proposes a frozen section collecting and transferring system, which sequentially comprises an unwinding roller 1, a pressing roller 2, and a winding roller 3, and further comprises an adhesive tape 4 movably wound on the unwinding roller 1, the pressing roller 2, and the winding roller 3.

[0030] A heat preservation channel 5 is arranged between the pressing roller 2 and the winding roller 3, a refrigeration device 6 is arranged in the heat preservation channel 5, a side wall of the heat preservation channel 5 is provided with a transfer port 12, and a driving assembly for clamping a glass slide is arranged outside the transfer port 12. The glass slide is parallel to the adhesive tape 4 at the transfer port 12, and the driving assembly drives the glass slide to enter and exit the transfer port 12.

[0031] In the frozen sectioning scene, a frozen sample is placed in a frozen environment, and the frozen sample is sectioned by using the relative movement between a cutter and the frozen sample. The frozen section cut by sectioning is collected by the adhesive tape 4 unwound from the unwinding roller 1, and then the frozen section is taken out of the frozen environment. The heat preservation channel 5 is arranged between the pressing roller 2 and the winding roller 3 and located on the winding and unwinding path of the adhesive tape 4, so as to preserve the temperature of the frozen sample after it is taken out of the frozen environment, and then the frozen section on the adhesive tape 4 is transferred to the glass slide at the transfer port 12 of the heat preservation channel 5.

[0032] Specifically, the driving assembly drives the glass slide to enter and exit the heat preservation channel 5 through the transfer port 12. The driving assembly can be a linear motor 14 cooperating with a clamp 15, the clamp is fixed on the output shaft of the linear motor 14, and the glass slide is clamped by the clamp 15. The driving assembly can also be a mechanical arm (not shown in the figure), which can directly clamp the glass slide.

[0033] When the frozen section is transferred from the adhesive tape 4 to the glass slide, the driving assembly is moved away from the transfer port 12, at which time the operator can take the glass slide off for microscopic imaging observation. Also, when it is necessary to sample a certain position in the frozen section using a sampling needle or to cut using a laser, the operation can be directly performed on the glass slide.

[0034] Specifically, as shown in Figure 2 and Figure 3 , the heat preservation channel 5 comprises a heat conduction pipe 7 and a heat preservation layer 8 wrapped outside the heat conduction pipe 7, and the refrigeration device 6 is located between the heat conduction pipe 7 and the heat preservation layer 8 and is fixed on the outer wall of the heat conduction pipe 7. The heat conduction pipe 7 comprises multiple pipe sections 9, and adjacent two pipe sections 9 are detachably connected. In addition, the cross section of the pipe section 9 is in the shape of a concave character.

[0035] The pipe section 9 is made of high-thermal-conductivity material, such as copper, iron, etc. The heat preservation layer 8 is made of low-thermal-conductivity material, such as heat preservation cotton, heat preservation foam, heat preservation adhesive tape 4, etc. Since the cross section of the heat conduction pipe 7 is in the shape of a concave character and its upper end surface is open, before wrapping the heat preservation layer 8, a copper adhesive tape 10 can be used to seal the upper end surface of the heat conduction pipe 7 to form a loop-shaped pipe section 9, and then the heat preservation layer 8 is formed by wrapping heat preservation cotton, heat preservation foam or heat preservation adhesive tape, etc. During the adjustment of the position of the adhesive tape 4 during the section collection process, the sample is easy to scratch the inner surface of the upper end of the heat conduction pipe 7, so the heat conduction pipe 7 is designed in the shape of a concave character and is matched with the copper adhesive tape 10 to form a loop shape, which facilitates the cleaning of the inner wall of the pipe section 9 after the heat preservation layer 8 and the copper adhesive tape 10 are removed, thereby avoiding the formation of a pollution source in the heat conduction pipe 7.

[0036] The multiple pipe sections 9 are assembled to form the heat conduction pipe 7, for example, two pipe sections 9 can be respectively installed on the two sides of a hinge by bolts to complete the assembly of the multiple pipe sections 9, and the parts at the joint are preferably made of the same material as the pipe section 9 to ensure the high-thermal-conductivity performance of the entire heat conduction pipe 7. During the assembly process, the installation angle of adjacent two pipe sections 9 and the installation number of the pipe sections 9 can be flexibly controlled to realize a heat conduction pipe 7 with a specific radian and length, thereby meeting the shape requirements of the heat preservation channel 5 under different heights or distances of the sectioning roller 2 and the winding roller 3.

[0037] As shown in Figure 4 , the joint between adjacent two pipe sections 9 is filled with high-thermal-conductivity material 17. Preferably, it is a nano-molecular colloidal material, such as thermal conductive silicone grease, thermal conductive silicone, etc.

[0038] Due to the surface roughness and other reasons, the joint between two pipe sections 9 may have areas that cannot be directly contacted, which reduces the temperature conduction efficiency between adjacent two pipe sections 9. By filling the gap at the joint with nano-molecular colloidal material with high thermal conductivity, the heat transfer is contact heat transfer, thereby improving the conduction efficiency of the temperature in the entire heat conduction pipe 7.

[0039] As Figure 2 shown, the refrigeration device 6 is located in the middle part of the heat conduction pipe 7, and can be selected from a semiconductor refrigeration sheet or a thermoelectric refrigeration, a magnetic refrigeration, a vapor compression refrigeration, a vapor absorption refrigeration, a vapor jet refrigeration, etc. The heat dissipation of the refrigeration device 6 is performed by water cooling, which can avoid the influence of mechanical vibration generated by direct heat dissipation on the sample slice. The refrigeration device 6 is directly arranged on the outer side wall of the heat conduction pipe 7 to perform refrigeration on the inside of the heat conduction pipe 7, and then the low temperature is conducted to both ends by using the high thermal conductivity of the heat conduction pipe 7, so as to finally realize the low temperature environment in the inside of the heat conduction pipe 7. The sample slice carried by the adhesive tape 4 is not easily firmly adhered to the adhesive tape 4 due to melting, and is also more conducive to the step of separating and transferring the sample slice from the adhesive tape 4 after the heat preservation channel 5.

[0040] In some embodiments, the heat conduction pipe 7 is provided with adjustable openings at both ends. The smaller the opening, the better the low temperature environment maintaining effect in the heat conduction pipe 7. By adjusting the opening size at both ends, the position adjustment of the adhesive tape 4, the different thicknesses of the frozen slices and the temperature requirements of different organ tissues can be adapted. When the pressing roller 2 and the winding roller 3 are located at different heights, the heights of both ends of the heat conduction pipe 7 are also different. Among them, B point is the position point of the refrigeration device 6, A is the position point of the low end opening of the heat conduction pipe 7, and C is the position point of the high end opening of the heat conduction pipe 7.

[0041] The openings at both ends of the heat conduction pipe 7 are completely opened. When the room temperature is 20.0℃ and the working temperature of the refrigeration device 6 is-30.0℃, temperature sensors are attached to the inner walls of the heat conduction pipe 7 at A, B and C points. When B point measures-26.2℃, A point measures-15.0℃ and C point measures-7.2℃. A temperature sensor is suspended in the upper part of the heat conduction pipe 7. When B point measures 0.5℃, A point measures-11.0℃ and C point measures 16.0℃.

[0042] The higher end opening of the heat conduction pipe 7 is closed, when the room temperature is 20℃, the working temperature of the refrigeration device 6 is-30℃, the temperature sensor is suspended in the heat conduction pipe 7, the B point is-10.8℃, the A point is-9.7℃, then the higher end opening is completely opened, the B point is-1℃, the A point is-13℃. It can be found that the temperature difference of the other two points is closer when the end of the heat conduction pipe 7 is closed, and the B point will gradually increase in temperature and the A point will gradually decrease in temperature due to the sinking of cold air after the opening of the section. In actual use, the space position between the adhesive tape 4 and the heat preservation channel 5 is not constant, and theoretically, the opening of the heat preservation channel 5 at both ends can be adjusted to the minimum to achieve the best heat preservation effect, but in different use scenarios, the space position of the adhesive tape 4 often needs to be adjusted. If the opening of the heat conduction pipe 7 at both ends is set to a fixed size and is small enough for the adhesive tape 4 to carry the sample, the best heat preservation effect can be achieved, but due to the limited size of the opening at both ends, it is difficult to correct and adjust the position of the adhesive tape 4, and it cannot adapt to the passing requirements of different thicknesses of frozen sections. The sections are easy to collide or scratch the end of the heat conduction pipe 7, causing sample contamination, affecting secondary transfer and imaging observation.

[0043] Therefore, the openings at both ends of the heat conduction pipe 7 are completely opened, and the gate 11 is arranged at the opening. The gate 11 is fixed on a one-dimensional moving platform, and the opening size of the heat conduction pipe 7 at both ends is controlled by driving the gate 11 to move through the one-dimensional moving platform, so as to realize adjustable opening size and meet the adjustment requirements of the adhesive tape 4 and the conveying requirements of samples of different thicknesses.

[0044] A white light LED lamp (not shown in the figure) is also fixed inside the heat conduction pipe 7. When the gate 11 is adjusted, the white light LED lamp is illuminated, and then the color of the light between the gate 11 and the adhesive tape 4 is observed. Since the gap between the gate 11 and the adhesive tape 4 is required to be small, the light gap method can be used for judgment. When the light between the gate 11 and the adhesive tape 4 is red, it means that the gap between the gate 11 and the adhesive tape 4 is about 1.5μm, and the opening size at this time is the minimum opening that meets the passing requirement of the adhesive tape 4.

[0045] The best storage temperature of frozen sections of different organs is different. For example, the best storage temperature of frozen sections of kidney tissue is below-25℃, and the best storage temperature of frozen sections of pancreas tissue is below-15℃. Therefore, the size of the opening at both ends can be adjusted to make the temperature inside the heat conduction pipe 7 meet the temperature requirements in different working environments, and the operation is more flexible.

[0046] As shown in Figure 5 Since the cutting of frozen sections is carried out in a frozen environment, the adhesive tape 4 can be an adhesive tape or an electrostatic tape, as shown in Figure 1As shown, when the tape 4 is an electrostatic tape, an electrostatic generator 16 needs to be installed between the unwinding roller 1 and the pressure roller 2 to charge the originally non-adhesive tape 4, forming an electrostatic tape. During the cutting and collecting process, the adhesive force / electrostatic attraction of the pressure roller 2 and the tape 4 is used to collect the cut frozen sections onto the tape 4. At this time, as long as the adhesive force between the frozen section and the slide is greater than the adhesive force / electrostatic attraction of the tape 4 on the frozen section, the transfer of the frozen section to the slide can be achieved.

[0047] like Figure 6 As shown, in some embodiments, an expansion material 13 is adhered inside the insulation channel 5. The expansion material 13 is located on the side of the tape 4 away from the transfer port 12. The expansion of the expansion material 13 keeps the frozen sections flat during the transfer process, effectively ensuring the transfer quality of the frozen sections. After the expansion material 13 expands, its edges will deform under pressure. Therefore, by supplying air to the expansion material 13 to make it expand, and then applying pressure to the tape 4, the side of the expansion material 13 in contact with the tape 4 will become flat, allowing the frozen sections to adhere smoothly to the glass slide, avoiding problems such as deformation and breakage of the frozen sections during transfer. The expansion material 13 can be a balloon, rubber ball, etc., supplied with air through a connected air tube, and a pressure valve is installed on the air tube. The air supply end is located outside the insulation channel 5 to avoid the mechanical vibration generated during operation affecting the slide collection and transfer process.

[0048] like Figure 7 As shown, when expansion is achieved by air supply, air is supplied directly through the air pipe connected to the expansion material 13, and the increase in air pressure, which is the final amount of air supplied to the expansion material 13, is monitored by a pressure valve. Specifically, the increase in air pressure by the pressure valve is P. Figure 7 In diagram (a), the initial positional relationship between the expanding material, the tape, and the glass slide is shown. Here, h1 represents the initial distance between the glass slide and the surface of the frozen section; 2a, 2b, and 2c represent the triaxial lengths of the expanding material 13 in the initial state, with 2b being the length perpendicular to the tape 4; h2 represents the shortest distance between the expanding material 13 and the tape 4 in the initial state, h2 ≥ 0; n is the amount of gas; r is the molar gas constant; and t represents the temperature of the gas. Ideally, when... The contact length between the expansion material 13 and the adhesive tape 4 is exactly equal to the length D of the frozen section. The larger the P value, the longer the contact length between the expansion material 13 and the adhesive tape 4, and the greater the force exerted by the expansion material 13 on the slide. Typically, the maximum pressure that a frozen section can withstand per square centimeter does not exceed 250 N. Therefore, the maximum value of P is limited to... The range can effectively avoid the damage to the frozen section caused by excessive air supply. In some scenarios, when the difference between the adsorption force of the slide on the frozen section and the adsorption force of the adhesive tape 4 on the frozen section is small, a larger air supply can be selected within the above range to apply additional pressure to the frozen section by the expanding material 13. Therefore, as long as a, b, c, D, h1 and h2 are determined, an air supply amount can be selected for the expanding material 13 within the above range according to the actual scene to achieve a wide range of self-adaptive pushing effect. Alternatively, the transfer situation can be observed when air is supplied within the above range, and if the expected state is reached, the air supply can be stopped in time without the need for manual repeated observation and adjustment.

[0049] As shown in Figure 7 Before expansion, the expanding material 13 is in the shape of an ellipsoid, and its volume is Figure 7 (b) represents the position between the expanded state and the adhesive tape and the slide, Figure 7 (c) represents the position between the expanded state and the adhesive tape and the slide during transfer. During transfer, the end close to the adhesive tape 4 is in close contact with the adhesive tape 4, so that the length of the axis of the expanding material 13 in the direction perpendicular to the adhesive tape 4 after expansion is h1+h2+2b, and the axis of the expanding material 13 parallel to the direction of the adhesive tape 4 is the long axis. When the cross section of the expanding material 13 at a distance of h1+h2+b from the long axis is cut, Figure 7 (d) shown, when the cross section is equal to the sample length D, it is considered that the close contact function is completed. At this time, the cross section of the expanding material 13 can be regarded as a circle with the same radius as the short axis of the ellipse (i.e. the axis perpendicular to the length direction of the adhesive tape 11), and the radius is When the cross section formed by the circle reaches D, the close contact effect can be achieved. In an ideal state, the expanding material 13 expands uniformly during the expansion process, that is, the expansion coefficients of the three axes are the same, so that the volume V2 after expansion is β 3 V1, The pressure that the pressure valve should lift is That is

[0050] The application also provides a method for collecting and transferring frozen sections, comprising the following steps:

[0051] The winding roller 3 starts to wind the adhesive tape 4, and the adhesive tape 4 is pasted with the frozen section at the sectioning roller 2, and the slide is clamped in the driving device. When the frozen section on the adhesive tape 4 moves to the transfer port 12 in the heat preservation channel 5, the driving device drives the slide into the transfer port 12;

[0052] The winding roller 3 stops winding the adhesive tape 4, and the frozen section is attached to the glass slide, so that the adhesion of the glass slide to the frozen section is greater than the adhesion of the adhesive tape to the frozen section, and the biological section can be smoothly transferred to the glass slide.

[0053] Specifically, during the attachment process, the frozen section can be pushed to approach the glass slide by the expansion of the expansion material 13, so that the attachment is more smooth, and the expansion of the expansion material 13 can also provide a pushing force during the transfer, so that the frozen section is more easily attached to the glass slide; or the glass slide can be directly driven to approach the frozen section by the driving device. When the adhesion of the glass slide to the frozen section is greater than the adhesion of the adhesive tape 4 to the frozen section, the transfer can be performed. Specifically, the glass slide can be heated before entering the transfer port 12 to generate a large temperature difference between the glass slide and the frozen section, thereby completing the adhesion; or the glass slide can be spin-coated with UV glue before the static transfer port 12, and then irradiated and cured by a UV lamp after the glass slide is attached to the frozen section, thereby completing the adhesion.

[0054] Further, before the adhesive tape 4 starts to adhere to the frozen section, the opening size of the heat conduction pipe 7 at both ends needs to be adjusted. Specifically, first, completely close the gate 11 and turn on the white light LED lamp. At this time, since the gate 11 is closed, the adhesive tape 4 will be pressed tightly in the heat conduction pipe 7, and then the gate 11 is gradually opened by the one-dimensional moving platform with a step distance of 0.1 μm, while the color of the transmitted light between the gate 11 and the adhesive tape 4 is observed by the naked eye. When the light color is red, it means that the gap between the gate 11 and the adhesive tape 4 is about 1.5 μm, that is, the friction between the gate 11 and the adhesive tape 4 is 0, and the opening size at this time is the minimum opening size that meets the requirement of the adhesive tape 4 passing through. Therefore, the one-dimensional moving platform stops moving, and the opening size at both ends of the heat conduction pipe 7 is determined.

[0055] By this section transfer method, the frozen section can be collected and transferred in the heat preservation channel 5, and before the section is collected, the opening size at both ends of the heat conduction pipe 7 can be adjusted by this method to meet the demand of the friction of the adhesive tape 40 passing through the heat preservation channel 5 in different space environments, while the opening size at both ends of the heat conduction pipe 7 is minimized as much as possible to achieve the best heat preservation effect. The white light LED lamp structure is simple, does not need to make large changes to the inside of the heat preservation channel 5, and has small volume, which will not affect the transportation of the adhesive tape 4. At the same time, this method can also directly use the naked eye to obtain relatively accurate results without the need for additional detection equipment.

[0056] The adsorption force of the slide to the frozen section is greater than the adsorption force of the adhesive tape to the frozen section, specifically: the slide is heated and warmed before entering the transfer port 12, so that the temperature difference is large enough, and the frozen section is stuck from the adhesive tape 4 to the slide by using the temperature difference between the slide and the frozen section, thereby realizing the transfer of the frozen section and improving the sticking force between the frozen section and the slide; the UV glue can also be spin-coated on the slide in advance, and when the frozen section is attached to the slide, the UV glue on the slide is irradiated by the ultraviolet lamp to solidify, so that the frozen section is attached to the slide. In this method, the ultraviolet lamp can be fixed inside the heat preservation channel 5.

[0057] Those skilled in the art will easily understand that the above description is only the preferred embodiment of the present application, and is not used to limit the present application, and any modification, equivalent replacement and improvement made within the spirit and principle of the present application shall be included in the protection scope of the present application.

Claims

1. A frozen slice receiving and transfer system, comprising, in sequence, an unwinding roller, a pressing roller, and a take-up roller, and further comprising an adhesive tape movably wound around the unwinding roller, the pressing roller, and the take-up roller, characterized in that, A heat-insulating channel is provided between the pressing roller and the winding roller. A cooling device is installed inside the heat-insulating channel. A transfer port is opened on the side wall of the heat-insulating channel. A driving assembly for clamping a glass slide is provided outside the transfer port. The glass slide is parallel to the tape at the transfer port. The driving assembly drives the glass slide in and out of the transfer port. An expansion material is adhered inside the heat-insulating channel. The expansion material is located on the side of the tape away from the transfer port. The expansion material is connected to an air pipe with a pressure valve for air supply. The air pressure P of the pressure valve needs to meet the following conditions: Where h1 represents the initial distance between the glass slide and the surface of the frozen section, a, b, and c represent half the distance of the triaxial length of the expansion material in the initial state, where the length of the frozen section is D, the length of the axis perpendicular to the tape is 2b, h2 represents the shortest distance between the expansion material and the tape in the initial state, h2 ≥ 0, n is the amount of gas, r is the molar gas constant, and t represents the temperature of the gas.

2. The wafer receiving and transfer system according to claim 1, characterized in that, The heat-insulating channel includes a heat-conducting pipe and a heat-insulating layer covering the heat-conducting pipe. The refrigeration device is located between the heat-conducting pipe and the heat-insulating layer and is fixed to the outer wall of the heat-conducting pipe.

3. The wafer receiving and transfer system according to claim 2, characterized in that, The heat-conducting pipe comprises multiple pipe sections, which are detachably connected to adjacent pipe sections, and the cross-section of the pipe is U-shaped.

4. The wafer receiving and transfer system according to claim 3, characterized in that, The pipe is made of a high thermal conductivity material, the insulation layer is made of a low thermal conductivity material, and the connection between two adjacent pipe sections is filled with a high thermal conductivity material.

5. The wafer transfer system according to claim 2, wherein the heat pipe has adjustable openings at both ends.

6. The wafer receiving and transfer system according to claim 5, characterized in that, The heat pipe is equipped with gates at both ends of its openings, and the gates are fixed on a one-dimensional moving platform.

7. The wafer receiving and transfer system according to claim 6, characterized in that, A white LED light is fixed inside the heat pipe.

8. A method for collecting and transferring frozen sections, characterized in that, Includes the following steps: The take-up roller starts to take up the tape, and the tape is attached to the transfer frozen slice at the pressure roller. At the same time, the glass slide is clamped in the drive device. When the frozen slice on the tape moves to the transfer port in the heat preservation channel, the drive device drives the glass slide into the transfer port. The take-up roller stops winding the tape, and the frozen section is bonded to the glass slide, so that the adhesion of the glass slide to the frozen section is greater than that of the tape to the frozen section, and the biological section is transferred flatly onto the glass slide.

9. The wafer receiving and transfer method according to claim 8, characterized in that, Before attaching the frozen section with tape, adjust the size of the openings at both ends of the heat pipe. Specifically, first, completely close the gate and turn on the white LED light, press the tape tightly inside the heat pipe, and then gradually open the gate using a one-dimensional moving platform with a step distance of 0.1μm. At the same time, observe the color of the transmitted light between the gate and the tape with the naked eye. When it turns red, stop moving the one-dimensional moving platform. The size of the openings at both ends of the heat pipe is then determined.

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