A postbiotic fermentation device and a fermentation method thereof

Abstract

The application provides a postbiotic fermentation device and a fermentation method thereof, and relates to the technical field of postbiotics. The technical scheme of the application provides a postbiotic fermentation device, which comprises a fermentation box, a culture medium preparation mechanism arranged in the fermentation box from top to bottom, a fermentation mechanism, a culture medium preparation mechanism, and a baffle. The culture medium preparation mechanism comprises a driving part and a feeding part. The driving part comprises a driving gear and a stirring main shaft fixed to the driving gear. The stirring main shaft is fixed to a stirring body and a first stirring rod from top to bottom. The shape of the stirring body is one of an umbrella shape and a semi-elliptical shape. The feeding part comprises an intermediate gear, a driven gear, a material conveying shaft, and a material box. An elastic output nozzle is fixed to the material box. The device can realize omnidirectional mixing under the action of the stirring body. After the second stirring and mixing, the fermentation substrate is uniformly mixed, and the growth of the strain is uniform during the fermentation process. The application also provides a postbiotic fermentation method. The method is simple, and the postbiotic strain is completely fermented.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of postbiotics, in particular to a postbiotic fermentation device and a fermentation method thereof. BACKGROUND

[0002] Postbiotics is a general term for probiotic components after processing, including bacterial cells and metabolites. Microbial scientific research has confirmed that postbiotics selected for their ability to enhance immunity are superior to live bacteria, and even after being treated by high temperature or gastrointestinal juice, they still have high physiological activity.

[0003] Postbiotics is a dry powder mixture obtained by freeze-drying the fermentation metabolites and probiotics after fermentation of various probiotics. Therefore, the fermentation process of probiotics will directly affect the activity of beneficial substances in the prepared postbiotics.

[0004] There are several main influencing factors in the fermentation process of postbiotics: the uniformity of each substance in the fermentation substrate, the fermentation temperature, and the oxygen content of the fermentation environment. These factors directly affect the fermentation effect of postbiotics. Therefore, a suitable fermentation device is crucial for obtaining postbiotics with good activity and beneficial properties. In the patent with application number 201920827193.6, a device for producing heat-sealed lactic acid bacteria fermentation production postbiotics is disclosed. The device includes a container with an inlet, an outlet, a sampling port, and an air supply pipe. A rotating shaft with stirring wings is located in the container, and a cold and hot water temperature control system is arranged outside the container. In this way, after the lactic acid bacteria and culture medium are poured into the container through the inlet, they are continuously stirred by the stirring wings and air supply pipe. When the sampled bacteria reach the fermentation termination condition, the cold and hot water temperature control system is used to heat the container, so that the active lactic acid bacteria in the container are heated into non-active bacteria, which are then discharged through the outlet. Then, the liquid is concentrated and evaporated by a concentration device, and the compound lactic acid bacteria and the postbiotics produced by fermentation are obtained. In this device, existing culture materials are directly used for fermentation, and the uniformity of the fermentation substrate cannot be guaranteed. The growth of bacterial colonies in different areas during fermentation may be different, causing local good growth and limited growth. In addition, the mixing device in the prior art often uses a stirring rod to mix, which can cause local accumulation of a certain material, resulting in uneven mixing. SUMMARY

[0005] The present application aims to overcome the above technical deficiencies and provide a postbiotic fermentation device and a fermentation method thereof, which solves the technical problems of poor uniformity of the fermentation substrate and uneven growth of bacterial colonies during fermentation in the prior art.

[0006] In order to achieve the above technical purposes, the technical scheme of the present application provides a postbiotic fermentation device, which comprises a fermentation box, a culture medium preparation mechanism arranged in the fermentation box from top to bottom, and a fermentation mechanism arranged in the fermentation box from top to bottom, a pullable baffle is arranged between the culture medium preparation mechanism and the fermentation mechanism, the culture medium preparation mechanism comprises a driving part and a feeding part engaged with the driving part, the driving part comprises a driving gear and a stirring main shaft fixed to the driving gear, the stirring main shaft is fixed to a stirring body and a first stirring rod from top to bottom, the shape of the stirring body is one of an umbrella shape and a semi-elliptical shape, the feeding part comprises an intermediate gear engaged with the driving gear, a driven gear engaged with the intermediate gear, a material conveying shaft fixed to the center of the driven gear, a material box fixed to the material conveying shaft and in communication, two or more elastic output nozzles are fixed to the material box, the elastic output nozzles can abut against the inner wall of the fermentation box, and the material output from the elastic output nozzles can be sprayed onto the surface of the stirring body, the fermentation mechanism comprises a fermentation cavity arranged at the lower part of the fermentation box, a feeding port, a discharging port, and a temperature control device arranged on the inner wall of the fermentation box.

[0007] More preferably, the number of the driving parts is 2-3.

[0008] More preferably, a material conveying through hole is arranged in the middle of the material conveying shaft, a feeding through hole in communication with one end of the material conveying through hole is arranged in the middle of the driven gear, and a feeding through hole in communication with the other end of the material conveying through hole is arranged on the top of the material box.

[0009] More preferably, a plurality of grooves are arranged on the surface of the stirring body.

[0010] More preferably, the radius of the driving gear is smaller than that of the driven gear.

[0011] More preferably, the radius of the driven gear is equal to 2 times the radius of the driving gear.

[0012] More preferably, the elastic output nozzles comprise first, second and third output nozzles with different vertical heights.

[0013] More preferably, the fermentation mechanism comprises a fermentation cavity arranged at the lower part of the fermentation box, a feeding port arranged on the side wall of the fermentation box and in communication with the fermentation cavity, a discharging port arranged at the bottom of the fermentation cavity, a temperature control device arranged on the inner wall of the fermentation box, and an oxygen supply device.

[0014] More preferably, a fermentation stirring part is arranged in the fermentation cavity, the fermentation stirring part comprises a stirring shaft and stirring paddles fixed to the stirring shaft.

[0015] A postbiotic fermentation method, the fermentation device used in the fermentation method is described above, and the fermentation method comprises the following steps.

[0016] S1, start the driving gear, add the raw materials for making the culture medium into the material box, and add different materials into different material boxes;

[0017] S2, pull out the baffle after the mixing is completed, so that the mixed culture medium raw materials enter the fermentation cavity, and then adjust the oxygen content and temperature in the fermentation cavity, and perform fermentation, the fermentation time is 24-36h, and the fermentation temperature is 32-35℃;

[0018] S3, after the fermentation is completed, the fermented materials in the fermentation cavity are output through the discharge port.

[0019] The working principle of the post-fermentation device is as follows:

[0020] Start the driving gear, and add different raw materials into different material boxes. Under the driving of the driving gear, the stirring body rotates, and the driven wheel also starts to rotate, so as to drive the material box to rotate. The rotation direction of the stirring body is the same as that of the material box, and when the circle where the stirring body and the material box are tangent, the rotation directions of the two are opposite, so that local accumulation of materials can be avoided. Different materials output from the material boxes of different driving parts fly in all directions under the action of the centrifugal force of the stirring body, and the first mixing of the materials is performed, and then the second mixing is performed under the action of the first stirring rod. Pull out the baffle, and the fermentation substrate falls into the fermentation cavity to perform fermentation.

[0021] Compared with the prior art, the device can realize omnidirectional mixing under the action of the stirring body, and then the fermentation substrate is uniformly mixed through the second stirring and mixing, and the growth of the strain is uniform in the fermentation process. The application also provides a post-fermentation method, which is simple and complete in fermentation of post-fermentation strains. BRIEF DESCRIPTION OF DRAWINGS

[0022] Figure 1 is a structure diagram of a post-fermentation device provided in the embodiment of the application;

[0023] Figure 2 is a structure diagram of a culture medium making mechanism of a post-fermentation device provided in the embodiment of the application;

[0024] Figure 3 is a structure diagram of a feeding part of a post-fermentation device provided in the embodiment of the application;

[0025] Figure 4 is a structure diagram of a material conveying shaft of a post-fermentation device provided in the embodiment of the application;

[0026] Figure 5is a structural schematic view of a material box of a postbiotic fermentation device provided in the embodiment of the present application during working;

[0027] Figure 6 is a structural schematic view of a stirring body of a postbiotic fermentation device provided in the embodiment of the present application.

[0028] Wherein; fermentation tank 1, culture medium preparation mechanism 2, driving part 21, driving gear 211, stirring spindle 212, stirring body 213, groove 2131, first stirring rod 214, feeding part 22, intermediate gear 221, driven gear 222, feeding through hole 2221, material transmission shaft 223, material transmission through hole 2231, material box 224, feeding through hole 2241, elastic output nozzle 225, first output nozzle 2251, second output nozzle 2252, third output nozzle 2253, fermentation mechanism 3, fermentation cavity 31, feeding port 32, discharging port 33, fermentation stirring part 34, stirring shaft 341, stirring paddle 342, temperature control equipment 35, oxygen supply equipment 36, baffle 4. DETAILED DESCRIPTION

[0029] In order to make the purpose, technical scheme and advantages of the present application more clear, the present application is further described in detail below in combination with the drawings and examples. It should be understood that the specific examples described herein are only used to explain the present application and do not limit the present application.

[0030] Example 1

[0031] Please refer to Figures 1 to 6The application discloses a postbiotic fermentation device, which comprises a fermentation box 1, a culture medium preparation mechanism 2 arranged in the fermentation box 1 from top to bottom, a fermentation mechanism 3, a pullable baffle 4 arranged between the culture medium preparation mechanism 2 and the fermentation mechanism 3, the culture medium preparation mechanism 2 comprises a driving part 21 and a feeding part 22 engaged with the driving part 21, the driving part 21 comprises a driving gear 211 and a stirring main shaft 212 fixed to the driving gear 211, the stirring main shaft 212 is fixed to a stirring body 213 and a first stirring rod 214 from top to bottom, the shape of the stirring body 213 can be one of an umbrella shape, a semi-elliptical shape, and the shape of the stirring body 213 in the embodiment is an umbrella shape, the feeding part 22 comprises an intermediate gear 221 engaged with the driving gear, a driven gear 222 engaged with the intermediate gear 221, a material conveying shaft 223 fixed to the center of the driven gear 222, a material box 224 fixed to the material conveying shaft 223 and in communication, two or more than two elastic output nozzles 225 are fixed to the material box 224, the elastic output nozzles 225 can abut against the inner wall of the fermentation box 1, the elasticity refers to that the elastic output nozzles 225 can be deformed when being extruded, that is, abutting against the inner wall of the fermentation box 1, so that the output nozzles no longer have the ability to output the material in the material box 224, when not being extruded by external objects, the elastic output nozzles 225 return to the original shape, the elastic output nozzles 225 can continue to normally output the material, the material output from the elastic output nozzles 225 can be sprayed to the surface of the stirring body 213, the fermentation mechanism 3 comprises a fermentation cavity 31 arranged at the lower portion of the fermentation box 1, a feeding port 32 arranged on the side wall of the fermentation box 1 and in communication with the fermentation cavity 31, a discharging port 33 at the bottom of the fermentation cavity 31, a temperature control device 35 arranged on the inner wall of the fermentation box 1 and an oxygen supply device 36. The number of the driving parts 21 is 2-3, and the number of the driving parts 21 can be increased, because the components of the fermentation substrate (culture medium) are often multiple, different components are arranged in different material boxes 224, and then the mixing of the materials is realized under the action of the stirring body 213 and the stirring shaft 341.

[0032] A material conveying through hole 2231 is arranged in the middle of the material conveying shaft 223, a feeding through hole 2221 in communication with one end of the material conveying through hole 2231 is arranged in the middle of the driven gear 222, and a feeding through hole 2241 in communication with the other end of the material conveying through hole 2231 is arranged at the top of the material box 224.

[0033] The surface of the stirring body 213 is provided with a plurality of grooves 2131. The grooves 2131 can temporarily store materials, and then under the action of the centripetal force, the materials will fall out of the grooves 2131 and then enter the lower part of the stirring box body when the stirring body 213 rotates.

[0034] The radius of the drive gear 211 is smaller than that of the driven gear 222. The radius of the driven gear 222 is equal to 2 times the radius of the drive gear 211. In order to make the rotational angular velocity of the material box 224 smaller than the angular velocity of the stirring body 213, so that the materials output by the elastic output nozzle 225 are scattered, the materials sprayed by the elastic output nozzles 225 at different vertical heights fall on different rotating track circles of the stirring body 213, so that the scattered materials are dispersed in different areas, and the materials of a certain material box 224 are not scattered in a certain area, causing the accumulation of a certain material and uneven stirring.

[0035] The elastic output nozzle 225 includes first, second and third output nozzles 2251, 2252 and 2253 with different vertical heights. The first output nozzle 2251 can be arranged at the top of the material box 224, the second output nozzle 2252 can be arranged at the middle position of the material box 224, and the third output nozzle 2253 can be arranged at the bottom of the material box 224, so that the elastic output nozzles 225 at different heights can be sprayed on different positions of the stirring body 213 under the action of the centrifugal force of the stirring body 213 and fly in different directions of the fermentation tank 1. In the embodiment, the number of the driving parts 21 is 3, the materials 1 output from the material box 224 of the first driving part 21 fall on the stirring body 213, and then the materials 2 and 3 output from the material boxes 224 of the second and third driving parts 21 also fall on the stirring body 213 in sequence or simultaneously, and then fly in different directions under the action of the centrifugal force, to perform the first mixing of the materials, and then perform the second mixing under the action of the first stirring rod 214, so that the final fermentation substrate material is uniformly distributed.

[0036] A postbiotic fermentation method, the fermentation device used in the fermentation method is described above, characterized in that it comprises the following steps:

[0037] S1, start the drive gear 211, add the raw materials for the culture medium to the material box 224, and add different materials to different material boxes 224;

[0038] S2, after the mixing is completed, pull out the baffle 4, so that the uniformly mixed culture medium raw materials enter the fermentation cavity 31, and then adjust the oxygen content and temperature in the fermentation cavity 31 to perform fermentation, the fermentation time is 24 h, and the fermentation temperature is 32℃;

[0039] S3, after the fermentation is completed, the fermented materials in the fermentation cavity 31 are output through the discharge port 33.

[0040] The working principle of the postbiotic fermentation device in this embodiment is as follows:

[0041] The driving gear 211 is started, and various raw materials for the culture medium are added to the different material boxes 224. Under the driving of the driving gear 211, the stirring body 213 rotates, and the driven gear also starts to rotate, thereby driving the material box 224 to rotate. The rotation direction of the stirring body 213 is the same as that of the material box 224, for example, both can rotate counterclockwise. When the stirring body 213 and the material box 224 are tangent, the rotation directions of the two are opposite, which can avoid local accumulation of the material. The output material 1 in the material box 224 of the first driving part 21 falls on the stirring body 213, and then the output materials 2 and 3 in the material boxes 224 of the second driving part 21 and the third driving part 21 also fall on the stirring body 213 in turn or simultaneously, and then fly in all directions under the action of centrifugal force, to perform the first mixing of the materials, and then perform the second mixing under the action of the first stirring rod 214. In this way, the final fermentation substrate material is very uniform. After the fermentation substrate is completely mixed, the baffle 4 is pulled out, and then the fermentation substrate falls into the fermentation cavity 31, the fermentation temperature and the fermentation environment are adjusted, and then the corresponding strain is transplanted in the feeding port 32. After the fermentation is completed, the discharge port 33 is discharged.

[0042] Comparative example

[0043] In this comparative example, the fermentation device in the application number 201920827193.6 is selected.

[0044] Lactobacillus is taken, and the same component fermentation substrate is used to ferment Lactobacillus by using the device in example 1 and the device in the comparative example, respectively, and the uniformity of the colonies after fermentation is observed. The specific situation is as follows

[0045]

[0046] The specific embodiments of the application described above do not constitute a limitation on the scope of protection of the application. Any various other corresponding changes and modifications made according to the technical concept of the application shall be included in the protection scope of the claims of the application.

Claims

1. A postbiotic fermentation device comprising a fermentation tank, a culture medium preparation mechanism, a fermentation mechanism disposed in the fermentation tank from top to bottom, and a pullable baffle disposed between the culture medium preparation mechanism and the fermentation mechanism, characterized in that, The culture medium making mechanism comprises a driving part, a feeding part engaged with the driving part; the driving part comprises a driving gear, a stirring main shaft fixed to the driving gear, the stirring main shaft is fixed to a stirring body and a first stirring rod from top to bottom, the stirring body is in one of an umbrella shape and a semi-elliptical shape, and a plurality of grooves are arranged on the surface of the stirring body; the feeding part comprises an intermediate gear engaged with the driving gear, a driven gear engaged with the intermediate gear, a material conveying shaft fixed to the center of the driven gear, and a material box fixed to and communicating with the material conveying shaft, two or more elastic output nozzles are fixed to the material box, the elastic output nozzles can abut against the inner wall of the fermentation tank, and the material output from the elastic output nozzles can be sprayed onto the surface of the stirring body.

2. A postbiotic fermentation device according to claim 1, characterized in that, The number of the driving parts is 2-3.

3. A postbiotic fermentation device according to claim 2, wherein, The material conveying shaft is provided with a material conveying through hole, the driven gear is provided with a feeding through hole in communication with one end of the material conveying through hole, and the top of the material box is provided with a feeding through hole in communication with the other end of the material conveying through hole.

4. The postbiotic fermentation device of claim 1, wherein, The radius of the driving gear is smaller than that of the driven gear.

5. A postbiotic fermentation device according to claim 4, wherein, The radius of the driven gear is equal to twice the radius of the driving gear.

6. A postbiotic fermentation device according to claim 1, wherein, The elastic output nozzles comprise first, second and third output nozzles with different vertical heights.

7. A postbiotic fermentation device according to claim 1, wherein, The fermentation mechanism comprises a fermentation cavity arranged at the lower part of the fermentation tank, a feeding port arranged on the side wall of the fermentation tank and in communication with the fermentation cavity, a discharging port in communication with the bottom of the fermentation cavity, a temperature control device arranged on the inner wall of the fermentation tank, and an oxygen supply device; the fermentation cavity is provided with a fermentation stirring part, which comprises a stirring shaft and stirring blades fixed to the stirring shaft.

8. A method for postbiotic fermentation, wherein the fermentation device used in the fermentation method is as described in claim 7, characterized in that, The method comprises the following steps: S1, start the driving gear, add raw materials for making culture medium into the material box, and add different materials into different material boxes; S2, after mixing, pull out the baffle to make the mixed culture medium raw materials enter the fermentation cavity, then adjust the oxygen content and temperature in the fermentation cavity, and perform fermentation, the fermentation time is 24-36h, and the fermentation temperature is 32-35℃; S3, after fermentation, output the fermented materials in the fermentation cavity through the discharging port.

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