Preparation method and application of bacterial cellulose / sodium alginate / SiO2 composite sponge material

Abstract

The present invention provides a method for preparing a bacterial cellulose / sodium alginate / SiO2 composite sponge material, comprising the following steps: separately preparing amino-modified mesoporous SiO2 microspheres and quaternized bacterial cellulose; adding the sodium alginate, quaternized bacterial cellulose, and amino-modified mesoporous SiO2 microspheres to distilled water, uniformly dispersing them, adding epichlorohydrin, adjusting the pH to 8.0-10.0, and reacting at 60-80°C to obtain a reaction liquid; and freeze-drying the reaction liquid to obtain the bacterial cellulose / sodium alginate / SiO2 composite sponge material. The present invention has the following beneficial effects: utilizing the mesopores of the SiO2 microspheres greatly increases the porosity and specific surface area of the composite sponge. The high porosity and large specific surface area enable rapid absorption of plasma, allowing blood cells to accumulate on the surface, thereby promoting blood coagulation on the wound surface.

Description

Technical Field

[0001] The invention relates to a preparation method of a bacterial cellulose / sodium alginate / SiO2 composite sponge material, and belongs to the field of porous materials and biomedical materials. Background Art

[0002] Blood plays an important role in human osmosis, acid-base balance, and temperature regulation. Studies have shown that rapid blood loss can lead to shock and even death. Therefore, during the rescue process, if bleeding can be stopped quickly and blood loss can be reduced, the survival rate will be greatly improved. Currently, a large number of hemostatic materials are widely used for rapid hemostasis, such as hemostatic gauze, hemostatic sponges, hemostatic powders, hemostatic sprays, and biogel adhesives. The current hemostatic mechanism mainly includes the following three aspects: 1) The hemostatic material contacts the wound surface and uses the charge on its surface to enrich and adhere to blood components, activate platelets, and trigger the coagulation cascade reaction; 2) Through physical or chemical means, it triggers vascular contraction or reduces blood flow velocity, increases the accumulation of platelets and blood cells at the wound, and promotes the coagulation reaction; 3) It mimics the function of platelets or blood cells and directly participates in the coagulation reaction.

[0003] Hemostatic sponges are suitable for treating large-scale bleeding, arterial rupture, or penetrating wounds. Therefore, the hemostatic performance of a hemostatic sponge is related to its specific surface area and water absorption capacity. Currently, hemostatic sponges are mainly prepared using methods such as freeze-drying, microwave puffing, and sacrificial template methods. For example, Chinese Patent CN110975001B discloses a chitosan-cellulose composite hemostatic sponge prepared by freeze-drying. The composite sponge's three-dimensional porous structure exhibits excellent liquid absorption, promoting red blood cell aggregation and accelerating blood coagulation. Chinese Patent CN109091699B utilizes high-pressure homogenization of oxidized cellulose powder, followed by freeze-thaw cycles of the oxidized cellulose suspension and freeze-drying to produce a cellulose hemostatic sponge. Both patents describe the preparation of organic porous sponge hemostatic materials. The question of how to combine organic and inorganic hemostatic materials to further enhance the material's hemostatic efficiency has become an urgent issue. Therefore, the question of how to construct a porous network structure to increase the sponge's specific surface area and water absorption capacity, enabling the sponge to better accumulate blood components and promote coagulation, remains a hot topic for future research. Summary of the Invention

[0004] In order to solve the above technical problems, the purpose of the present invention is to provide a preparation method and use of a bacterial cellulose / sodium alginate / SiO2 composite sponge material.

[0005] The present invention is achieved through the following technical solutions:

[0006] A method for preparing a bacterial cellulose / sodium alginate / SiO2 composite sponge material comprises the following steps:

[0007] Aminated mesoporous SiO2 microspheres and quaternized bacterial cellulose were prepared respectively;

[0008] Sodium alginate, the quaternized bacterial cellulose, and the amino-modified mesoporous SiO2 microspheres are added to distilled water, uniformly dispersed, and then epichlorohydrin is added to adjust the pH to 8.0-10.0, and reacted at 60-80° C. to obtain a reaction liquid;

[0009] The reaction solution is freeze-dried to obtain the bacterial cellulose / sodium alginate / SiO2 composite sponge material.

[0010] As a preferred embodiment, the preparation method of the amino-modified mesoporous SiO2 microspheres is as follows:

[0011] Cellulose acetate was added to tetrahydrofuran and dissolved, tetraethyl orthosilicate was added dropwise, the pH value was adjusted to 5-6, glycerol at a temperature of 40°C was added, and homogenization was performed at 50°C to obtain an emulsion;

[0012] After quenching the emulsion at -30 to -10°C, the tetrahydrofuran is washed off with an ice-water mixture, and then hydrolyzed with an ethanol solution of NaOH to obtain cellulose / SiO2 composite microspheres;

[0013] calcining the cellulose / SiO2 composite microspheres at 450-550° C. to obtain mesoporous SiO2 microspheres;

[0014] The mesoporous SiO2 microspheres are acidified with hydrochloric acid to obtain acidified mesoporous SiO2 microspheres, the acidified mesoporous SiO2 microspheres are dispersed in toluene, 3-aminopropyltriethoxysilane is added dropwise, and after reflux reaction, the mixture is washed with ethanol and dried to obtain the amino-modified mesoporous SiO2 microspheres.

[0015] As a preferred embodiment, the mass ratio of tetrahydrofuran to glycerol in the emulsion is (1-3): (3-9), the mass ratio of cellulose acetate to tetraethyl orthosilicate is (2-5): (1-3); and the mass ratio of the acidified mesoporous SiO2 microspheres to 3-aminopropyltriethoxysilane is (1-2): (3-6).

[0016] As a preferred embodiment, the preparation method of the quaternized bacterial cellulose is:

[0017] The bacterial cellulose is added to a mixture of deionized water and isopropyl alcohol and dispersed uniformly, and then a 10% by mass sodium hydroxide solution is added. 2,3-epoxypropyltrimethylammonium chloride is added at 65° C. After the reaction, the reactant is centrifuged, washed, and dried to obtain the quaternized bacterial cellulose.

[0018] As a preferred embodiment, the mass ratio of the bacterial cellulose to 2,3-epoxypropyltrimethylammonium chloride is (1-2):(6-9).

[0019] As a preferred embodiment, the bacterial cellulose is from the genus Acetobacter xylinum.

[0020] As a preferred embodiment, the mass ratio of the quaternized bacterial cellulose, sodium alginate and amino-modified mesoporous SiO2 microspheres is (3-6): (2-4): (1-2).

[0021] A bacterial cellulose / sodium alginate / SiO2 composite sponge material obtained by the above-mentioned preparation method.

[0022] A use of the bacterial cellulose / sodium alginate / SiO2 composite sponge material as described above in hemostatic materials.

[0023] The mechanism of the present invention is:

[0024] A cellulose acetate solution is prepared, and tetraethyl orthosilicate is added. The tetraethyl orthosilicate is hydrolyzed under acidic conditions to produce silica. Glycerol is added and stirred in a homogenizer. Self-emulsification forms an emulsion, which is then freeze-dried. The glycerol and water are removed to produce cellulose / SiO2 composite microspheres. The composite microspheres are then calcined to form mesoporous SiO2 microspheres. Finally, 3-aminopropyltriethoxysilane is reacted with the mesoporous SiO2 microspheres to introduce amino groups onto the microsphere surface.

[0025] Under alkaline conditions, bacterial cellulose is reacted with 2,3-epoxypropyltrimethylammonium chloride to quaternize the cellulose molecular chain so that the molecular chain carries ammonium ions.

[0026] Quaternized bacterial cellulose, sodium alginate and amino-modified mesoporous SiO2 microspheres were blended, cross-linked with epichlorohydrin under alkaline conditions, and finally freeze-dried to obtain bacterial cellulose / sodium alginate / SiO2 composite sponge.

[0027] Compared with the prior art, the present invention has the following beneficial effects:

[0028] 1) The mesopores of SiO2 microspheres are utilized to greatly improve the porosity and specific surface area of the composite sponge. The high porosity and large specific surface area can quickly absorb plasma, causing blood cells to accumulate on the surface, thereby promoting blood coagulation on the wound surface.

[0029] 2) Introduce amino groups to the surface of SiO2 microspheres. Although amino groups are uncharged, the nitrogen atom on the single amino group has a pair of lone electrons that can bind H + , forming positively charged NH3 +The positive charge of the composite material is increased, accelerating the coagulation process through the endogenous pathway. After the bacterial cellulose is quaternized, ammonium ions are introduced, and the cations agglomerate red blood cells to form thrombi, promoting hemostasis.

[0030] 3) Quaternized bacterial cellulose, sodium alginate and amino-modified mesoporous SiO2 microspheres are compounded and finally freeze-dried to obtain a sponge-like composite material. The high water absorption and high porosity structure of the sponge structure can quickly absorb plasma, thereby promoting blood coagulation on the wound surface and improving hemostatic performance. BRIEF DESCRIPTION OF THE DRAWINGS

[0031] Other features, objects and advantages of the present invention will become more apparent upon reading the detailed description of non-limiting embodiments with reference to the following drawings:

[0032] Figure 1 This is a scanning electron microscope image of the bacterial cellulose / sodium alginate / SiO2 composite sponge material prepared in Example 1 of the present invention. DETAILED DESCRIPTION

[0033] The present invention will be described in detail below with reference to specific embodiments. The following embodiments will help those skilled in the art to further understand the present invention, but are not intended to limit the present invention in any form. It should be noted that, for those skilled in the art, several variations and improvements can be made without departing from the scope of the present invention. These all fall within the scope of protection of the present invention.

[0034] The bacterial cellulose selected in the present invention is produced by Hainan Yide Food Co., Ltd. and belongs to the genus Acetobacter xylinum.

[0035] Example 1

[0036] This embodiment relates to a method for preparing a bacterial cellulose / sodium alginate / SiO2 composite sponge material, which specifically includes the following steps:

[0037] (1) Preparation of amino-modified mesoporous SiO2 microspheres

[0038] 0.5g of cellulose acetate (CA) was added to 50g of tetrahydrofuran (THF) solvent and magnetically stirred at 60°C for 2 hours to completely dissolve it. 0.3g of tetraethyl orthosilicate was added dropwise to the solution and stirred for 2 hours. The pH was adjusted to 5-6 with 0.1mol / L hydrochloric acid and stirred for another 2 hours. 150g of glycerol at 40°C was added to the mixed solution and stirred at 50°C for 10 minutes to form an emulsion. The emulsion was placed in a 2L beaker and placed in a pre-cooled -15°C refrigerator. It was quenched for 3 hours. After quenching, 1L of ice-water mixture was quickly added to the beaker. The water was changed every 8 hours for 2 days. The sample was then immersed in 0.1mol / L sodium hydroxide ethanol solution for 24 hours to convert the cellulose acetate into cellulose. The sample was then washed with distilled water and freeze-dried for 24 hours to obtain cellulose / SiO2 composite microspheres. The composite microspheres were calcined at 500°C for 5 hours to obtain mesoporous SiO2 microspheres.

[0039] 0.2 g of mesoporous SiO2 microspheres were added to 50 mL of 3 mol / L hydrochloric acid and soaked at room temperature for 12 h. The mixture was then washed with distilled water, dried, and oven-dried to obtain acidified mesoporous SiO2 microspheres. The acidified mesoporous SiO2 microspheres were dispersed in 100 mL of toluene, and 0.6 g of 3-aminopropyltriethoxysilane was added dropwise to the dispersion. The mixture was refluxed at 100°C for 12 h. The mixture was then washed with ethanol and dried to obtain amino-modified mesoporous SiO2 microspheres.

[0040] (2) Quaternized bacterial cellulose

[0041] Weigh 0.5g of bacterial cellulose and disperse it in 8mL of deionized water and 20mL of isopropanol. Then, add 10mL of a 10% sodium hydroxide solution. Add 4g of 2,3-epoxypropyltrimethylammonium chloride at 65°C and react with magnetic stirring for 10 hours. The reaction mixture is centrifuged, washed, and dried to obtain quaternized bacterial cellulose.

[0042] (3) Preparation of bacterial cellulose / sodium alginate / SiO2 composite sponge

[0043] 0.3 g of quaternized bacterial cellulose, 0.2 g of sodium alginate, and 0.2 g of amino-modified mesoporous SiO2 microspheres were added to 200 mL of distilled water and shaken in a 60°C water bath for 5 hours. Next, 0.1 g of epichlorohydrin was added, and the pH was adjusted to 9.0 with sodium hydroxide. The mixture was allowed to react at 70°C for 3 hours. After the reaction, the reaction solution was poured into a Petri dish and freeze-dried to obtain a bacterial cellulose / sodium alginate / SiO2 composite sponge.

[0044] The morphology of the bacterial cellulose / sodium alginate / SiO2 composite sponge material prepared in Example 1 is as follows: Figure 1As shown in the figure, the composite material has a porous structure consisting of large pores and small pores. The diameter of the large pores is 3-5 μm, and the diameter of the small pores is 300-500 nm. The porosity of the bacterial cellulose / sodium alginate / SiO2 composite sponge material is 85.1%, and the specific surface area is 8.12 m 2 The blood coagulation index (BCI) was used to evaluate blood coagulation in vitro, using the absorbance of the hemoglobin solution to determine the blood clotting rate. The BCI of the bacterial cellulose / sodium alginate / SiO2 composite sponge was 39.1%.

[0045] Example 2

[0046] This embodiment relates to a method for preparing a bacterial cellulose / sodium alginate / SiO2 composite sponge material, which specifically includes the following steps:

[0047] (1) Preparation of amino-modified mesoporous SiO2 microspheres

[0048] 0.3g of cellulose acetate (CA) was added to 40g of tetrahydrofuran (THF) solvent and magnetically stirred at 60°C for 2 hours to completely dissolve it. 0.3g of tetraethyl orthosilicate was dropwise added to the solution and stirred for 2 hours. The pH was adjusted to 5-6 with 0.1mol / L hydrochloric acid and stirred for another 2 hours. 160g of glycerol at 40°C was added to the mixed solution and stirred at 50°C for 10 minutes to form an emulsion. The emulsion was placed in a 2L beaker and placed in a pre-cooled -30°C refrigerator. It was quenched for 5 hours. After quenching, 1L of ice-water mixture was quickly added to the beaker. The water was changed every 8 hours for 2 days. The sample was then immersed in 0.1mol / L sodium hydroxide ethanol solution for 24 hours to convert the cellulose acetate into cellulose. The sample was then washed with distilled water and freeze-dried for 24 hours to obtain cellulose / SiO2 composite microspheres. The composite microspheres were calcined at 550°C for 5 hours to obtain mesoporous SiO2 microspheres.

[0049] 0.3 g of mesoporous SiO2 microspheres were added to 50 mL of 3 mol / L hydrochloric acid and soaked at room temperature for 12 h. The mixture was then washed with distilled water, dried, and oven-dried to obtain acidified mesoporous SiO2 microspheres. The acidified mesoporous SiO2 microspheres were dispersed in 100 mL of toluene, and 0.7 g of 3-aminopropyltriethoxysilane was added dropwise to the dispersion. The mixture was refluxed at 100°C for 12 h. The mixture was then washed with ethanol and dried to obtain amino-modified mesoporous SiO2 microspheres.

[0050] (2) Quaternized bacterial cellulose

[0051] Weigh 0.4 g of bacterial cellulose and disperse it in 8 mL of deionized water and 20 mL of isopropanol. Then, add 10 mL of a 10% sodium hydroxide solution. Add 3.5 g of 2,3-epoxypropyltrimethylammonium chloride at 65°C and react with magnetic stirring for 10 hours. The reaction mixture is centrifuged, washed, and dried to obtain quaternized bacterial cellulose.

[0052] (3) Preparation of bacterial cellulose / sodium alginate / SiO2 composite sponge

[0053] 0.4 g of quaternized bacterial cellulose, 0.3 g of sodium alginate, and 0.25 g of amino-modified mesoporous SiO2 microspheres were added to 200 mL of distilled water and shaken in a 60°C water bath for 5 hours. Next, 0.1 g of epichlorohydrin was added, and the pH was adjusted to 9.0 with sodium hydroxide. The mixture was allowed to react at 70°C for 3 hours. After the reaction, the solution was poured into a Petri dish and freeze-dried to obtain a bacterial cellulose / sodium alginate / SiO2 composite sponge.

[0054] The porosity of the bacterial cellulose / sodium alginate / SiO2 composite sponge material prepared in Example 2 was 85.9%, and the specific surface area was 8.22 m 2 The blood coagulation index (BCI) was used to evaluate blood coagulation in vitro, using the absorbance of the hemoglobin solution to determine the blood clotting rate. The BCI of the bacterial cellulose / sodium alginate / SiO2 composite sponge was 40.1%.

[0055] Example 3

[0056] This embodiment relates to a method for preparing a bacterial cellulose / sodium alginate / SiO2 composite sponge material, which specifically includes the following steps:

[0057] (1) Preparation of amino-modified mesoporous SiO2 microspheres

[0058] 0.4g of cellulose acetate (CA) was added to 50g of tetrahydrofuran (THF) solvent and magnetically stirred at 60°C for 2h to completely dissolve. 0.2g of tetraethyl orthosilicate was dropwise added to the solution and stirred for 2h. The pH was adjusted to 5-6 with 0.1mol / L hydrochloric acid and stirred for another 2h. 150g of glycerol at 40°C was added to the mixed solution and stirred at 50°C for 10min to form an emulsion. The emulsion was placed in a 2L beaker and placed in a pre-cooled -20°C refrigerator. It was quenched for 5h. After quenching, 1L of ice-water mixture was quickly added to the beaker. The water was changed every 8h for 2 days. The sample was then immersed in 0.1mol / L sodium hydroxide ethanol solution for 24h to convert the cellulose acetate into cellulose. The sample was then washed with distilled water and freeze-dried for 24h to obtain cellulose / SiO2 composite microspheres. The composite microspheres were calcined at 500°C for 5h to obtain mesoporous SiO2 microspheres.

[0059] 0.3 g of mesoporous SiO2 microspheres were added to 50 mL of 3 mol / L hydrochloric acid and soaked at room temperature for 12 h. The mixture was then washed with distilled water, dried, and oven-dried to obtain acidified mesoporous SiO2 microspheres. The acidified mesoporous SiO2 microspheres were dispersed in 100 mL of toluene, and 0.6 g of 3-aminopropyltriethoxysilane was added dropwise to the dispersion. The mixture was refluxed at 100°C for 12 h. The mixture was then washed with ethanol and dried to obtain amino-modified mesoporous SiO2 microspheres.

[0060] (2) Quaternized bacterial cellulose

[0061] Weigh 0.5 g of bacterial cellulose and disperse it in 8 mL of deionized water and 20 mL of isopropanol. Then, add 10 mL of a 10% sodium hydroxide solution. Add 4.5 g of 2,3-epoxypropyltrimethylammonium chloride at 65°C and react with magnetic stirring for 10 hours. The reaction mixture is centrifuged, washed, and dried to obtain quaternized bacterial cellulose.

[0062] (3) Preparation of bacterial cellulose / sodium alginate / SiO2 composite sponge

[0063] 0.3 g of quaternized bacterial cellulose, 0.25 g of sodium alginate, and 0.2 g of amino-modified mesoporous SiO2 microspheres were added to 200 mL of distilled water and shaken in a 60°C water bath for 5 hours. Next, 0.1 g of epichlorohydrin was added, and the pH was adjusted to 9.0 with sodium hydroxide. The mixture was allowed to react at 70°C for 3 hours. After the reaction, the reaction solution was poured into a Petri dish and freeze-dried to obtain a bacterial cellulose / sodium alginate / SiO2 composite sponge.

[0064] The porosity of the bacterial cellulose / sodium alginate / SiO2 composite sponge material prepared in Example 2 is 88.4%, and the specific surface area is 9.15 m 2 The blood coagulation index (BCI) was used to evaluate blood coagulation in vitro, using the absorbance of the hemoglobin solution to determine the blood clotting rate. The BCI of the bacterial cellulose / sodium alginate / SiO2 composite sponge was 38.3%.

[0065] Comparative Example 1

[0066] The difference from Example 1 is that in step 3), "mesoporous SiO2 microspheres" are used instead of "amino-modified mesoporous SiO2 microspheres". The other conditions remain unchanged to obtain a bacterial cellulose / sodium alginate / SiO2 composite sponge material. The porosity of the composite material is 80.9% and the specific surface area is 7.99m 2 The blood coagulation index (BCI) was used to evaluate blood coagulation in vitro, using the absorbance of the hemoglobin solution to determine the blood clotting rate. The BCI of the bacterial cellulose / sodium alginate / SiO2 composite sponge was 48.2%.

[0067] Comparative Example 2

[0068] The difference from Example 1 is that in step 3), "bacterial cellulose" is used instead of "quaternized bacterial cellulose", and the other conditions remain unchanged to obtain a bacterial cellulose / sodium alginate / SiO2 composite sponge material. The porosity of the composite material is 84.1%, and the specific surface area is 8.24m 2 The blood coagulation index (BCI) was used to evaluate blood coagulation in vitro, using the absorbance of the hemoglobin solution to determine the blood clotting rate. The BCI of the bacterial cellulose / sodium alginate / SiO2 composite sponge was 47.1%.

[0069] Comparative Example 3

[0070] The difference from Example 1 is that in step 3), no amino-modified mesoporous SiO2 microspheres are added, and finally a bacterial cellulose / sodium alginate composite sponge material is obtained. The porosity of the composite material is 76.1%, and the specific surface area is 6.24 m 2 The blood coagulation index (BCI) was used to evaluate blood coagulation in vitro, using the absorbance of the hemoglobin solution to determine the blood clotting rate. The BCI of the bacterial cellulose / sodium alginate / SiO2 composite sponge was 55.1%.

[0071] The above describes the specific embodiments of the present invention. It should be understood that the present invention is not limited to the above specific embodiments, and those skilled in the art may make various variations or modifications within the scope of the claims, which do not affect the essence of the present invention.

Claims

1. A method for preparing a bacterial cellulose / sodium alginate / SiO2 composite sponge material, characterized in that: The steps include: Aminated mesoporous SiO2 microspheres and quaternized bacterial cellulose were prepared respectively; Sodium alginate, the quaternized bacterial cellulose, and the amino-modified mesoporous SiO2 microspheres are added to distilled water, uniformly dispersed, and then epichlorohydrin is added to adjust the pH to 8.0-10.0, and reacted at 60-80° C. to obtain a reaction liquid; The reaction solution is freeze-dried to obtain the bacterial cellulose / sodium alginate / SiO2 composite sponge material.

2. The method for preparing the bacterial cellulose / sodium alginate / SiO2 composite sponge material according to claim 1, wherein: The preparation method of the amino-modified mesoporous SiO2 microspheres is as follows: Cellulose acetate was added to tetrahydrofuran and dissolved, tetraethyl orthosilicate was added dropwise, the pH value was adjusted to 5-6, glycerol at a temperature of 40°C was added, and homogenization was performed at 50°C to obtain an emulsion; After quenching the emulsion at -30 to -10°C, the tetrahydrofuran is washed off with an ice-water mixture, and then hydrolyzed with an ethanol solution of NaOH to obtain cellulose / SiO2 composite microspheres; calcining the cellulose / SiO2 composite microspheres at 450-550° C. to obtain mesoporous SiO2 microspheres; The mesoporous SiO2 microspheres are acidified with hydrochloric acid to obtain acidified mesoporous SiO2 microspheres, the acidified mesoporous SiO2 microspheres are dispersed in toluene, 3-aminopropyltriethoxysilane is added dropwise, and after reflux reaction, the mixture is washed with ethanol and dried to obtain the amino-modified mesoporous SiO2 microspheres.

3. The method for preparing the bacterial cellulose / sodium alginate / SiO2 composite sponge material according to claim 2, wherein: The mass ratio of tetrahydrofuran to glycerol in the emulsion is (1-3): (3-9), the mass ratio of cellulose acetate to tetraethyl orthosilicate is (2-5): (1-3); and the mass ratio of the acidified mesoporous SiO2 microspheres to 3-aminopropyltriethoxysilane is (1-2): (3-6).

4. The method for preparing the bacterial cellulose / sodium alginate / SiO2 composite sponge material according to claim 1, wherein: The preparation method of the quaternized bacterial cellulose is as follows: The bacterial cellulose is added to a mixture of deionized water and isopropyl alcohol and dispersed uniformly, and then a 10% by mass sodium hydroxide solution is added. 2,3-epoxypropyltrimethylammonium chloride is added at 65° C. After the reaction, the reactant is centrifuged, washed, and dried to obtain the quaternized bacterial cellulose.

5. The method for preparing the bacterial cellulose / sodium alginate / SiO2 composite sponge material according to claim 4, characterized in that: The mass ratio of the bacterial cellulose to 2,3-epoxypropyltrimethylammonium chloride is (1-2):(6-9).

6. The method for preparing the bacterial cellulose / sodium alginate / SiO2 composite sponge material according to claim 4, characterized in that: The bacterial cellulose is of the genus Acetobacter xylinum.

7. The method for preparing the bacterial cellulose / sodium alginate / SiO2 composite sponge material according to claim 1, wherein: The mass ratio of the quaternized bacterial cellulose, sodium alginate and amino mesoporous SiO2 microspheres is (3-6): (2-4): (1-2).

8. A bacterial cellulose / sodium alginate / SiO2 composite sponge material obtained by the preparation method according to claim 1.

9. Use of the bacterial cellulose / sodium alginate / SiO2 composite sponge material according to claim 8 in a hemostatic material.

Images