A cell sampling device and method of manufacturing the same

Abstract

The application relates to the technical field of biological medicine, and discloses a cell sampling device and a manufacturing method thereof. Six groups of sampling needle tubes which can be self-adjusted are arranged, the needle head of each group of sampling needle tubes can be self-adjusted, cell sampling work of multiple different depth skin layers can be completed at one time, the sampling depth can be accurately controlled by adjusting a positioning mechanism, the traditional sampling device and method are more accurate, a gel storage chamber and a gel pump-out mechanism are arranged, the gel storage chamber stores sterilized hemostatic gel, the gel pump-out mechanism pumps the sterilized hemostatic gel in the gel storage chamber into a gel laying groove, the needle head is completely covered, the wound can be quickly covered after the needle head is withdrawn, hemostasis and disinfection are realized, and hemostasis and disinfection are more convenient.

Description

Technical Field

[0001] This invention relates to the field of biomedical technology, specifically to a cell sampling device and its manufacturing method. Background Technology

[0002] In biomedical research, microscopy is often used to detect and analyze the structural and functional changes of cells, providing a reference for clinical diagnosis. Cell sampling devices are required when collecting cell pathology specimens.

[0003] Traditional cell sampling devices struggle to accurately determine the sampling depth and volume when collecting subcutaneous cells. Furthermore, collecting cells at different depths requires multiple sampling sessions, which is not only cumbersome but also causes more pain for the patient. Additionally, after collection, disinfection and hemostasis pressure are required using hemostatic cotton, making the process complex. Summary of the Invention

[0004] The purpose of this invention is to provide a cell sampling device and its manufacturing method to solve the problem mentioned in the background art that current traditional cell sampling devices are difficult to accurately locate the sampling depth and sampling volume.

[0005] To achieve the above objectives, the present invention provides the following technical solution: a cell sampling device and its manufacturing method, comprising a cell sampling pen, one end of which is fitted with a sampling sleeve, a pressure spring at the right end of the sampling sleeve, an adjustable syringe holder inside the cell sampling pen, a sampling needle connected to the adjustable syringe holder via a needle mounting structure, an adjustment and positioning mechanism at the right end of the adjustable syringe holder, a needle extension hole at the left end of the cell sampling pen, a gel storage chamber inside the cell sampling pen, a disinfectant hemostatic gel stored in the gel storage chamber, a threaded cap connected to the right end of the cell sampling pen via a thread, and a gel pumping mechanism at the left end of the gel storage chamber.

[0006] Furthermore, the adjustable syringe holder, sampling needle tube, and needle tube extension hole are provided in six sets, and the six sets of adjustable syringe holders, sampling needle tubes, and needle tube extension holes are evenly distributed at 60° intervals.

[0007] Furthermore, the adjustment and positioning mechanism includes a first adjustment groove. Six sets of parallel first adjustment grooves are formed on the outer wall of the cell sampling pen. Six sets of parallel second adjustment grooves are formed on the sampling sleeve corresponding to the first adjustment grooves. An adjustment slider is movably installed on the right end of the adjustable syringe holder. The adjustment slider passes through the first and second adjustment grooves. A telescopic rod is provided at one end of the adjustment slider. A telescopic groove is formed on the right end of the adjustable syringe holder. The telescopic rod is inserted into the telescopic groove. A positioning spring is provided in the telescopic groove. Slider positioning teeth are machined on both sides of the adjustment slider. Positioning racks are machined on both sides of the first adjustment groove. The width of the slider positioning teeth and the positioning racks is less than the compression stroke of the telescopic rod.

[0008] Furthermore, the needle mounting structure includes a first sector-shaped locking block and a second sector-shaped locking block. The first sector-shaped locking block is machined on the inner side of the tail of the sampling needle tube, and the second sector-shaped locking block is machined on the left end of the adjustable syringe holder. The second sector-shaped locking block is composed of two sets of centrally symmetrical 90° sector-shaped locking blocks, and the first sector-shaped locking block is composed of two sets of centrally symmetrical 60° sector-shaped locking blocks.

[0009] Furthermore, a syringe piston is provided inside the sampling syringe, a piston rod is fixedly connected to one end of the syringe piston, a fan-shaped fixing block is fixedly connected to one end of the piston rod, a fixing block is engaged in the arc-shaped groove on the adjustable syringe holder, a fan-shaped fixing groove is opened in the fixing block to engage with the fan-shaped fixing block, a sampling slider is fixedly connected to one side of the fixing block, and the sampling slider passes through the first adjustment groove and the second adjustment groove.

[0010] Furthermore, the piston rod has a cross-shaped cross section, and an anti-rotation limiting plate is provided at the right end of the sampling needle tube, through which the piston rod passes.

[0011] Furthermore, the gel pumping mechanism includes a gel pumping tube, the left end of the gel storage chamber is connected to the gel pumping tube, the left end of the gel pumping tube is connected to a gel pushing piston, a first one-way valve is provided inside the gel pushing piston, a piston tube is connected to the inner side of the left end of the sampling sleeve, the gel pushing piston is located inside the piston tube, a second one-way valve is provided at the left end of the piston tube, the first one-way valve has a passage direction from right to left, the second one-way valve has a passage direction from right to left, and a gel spreading groove is provided at the outlet of the left end of the piston tube.

[0012] Furthermore, the threaded cap has low airtightness with the cell sampling pen, or the threaded cap has ventilation openings during construction.

[0013] The present invention provides another technical solution, comprising the following steps:

[0014] S1. Aseptic manufacturing: First, the components of the sampling device are processed. After production, dust removal, cleaning and sterilization are carried out. Then, the parts are assembled in an aseptic environment.

[0015] S2. Production of disinfectant hemostatic gel: Fibrin monomers and neutralization buffer are reacted to prepare fibrin polymer. Neutralization buffer is added at a 1:1 ratio. Then, 1%-5% glycerol is added to the fibrin polymer to adjust the consistency. After that, bio-glue is added at a 1:1 ratio to form a hemostatic gel. Finally, 10% antibiotics are added to the hemostatic gel.

[0016] S3. Gel filling: Under aseptic conditions, the disinfectant hemostatic gel produced in step 2 is filled into the sampling device produced in step 1, and then aseptically packaged.

[0017] Compared with the prior art, the beneficial effects of the present invention are:

[0018] 1. The present invention discloses a cell sampling device and its manufacturing method. By setting up six sets of self-adjustable sampling needles, the needle extension length of each set of sampling needles can be adjusted independently, so that cell sampling of multiple cortical layers at different depths can be completed in one sampling. Moreover, the sampling depth can be precisely controlled by adjusting the positioning mechanism, which is more accurate than traditional sampling devices and methods.

[0019] 2. The present invention provides a cell sampling device and its manufacturing method. By setting up a gel storage chamber and a gel pumping mechanism, the gel storage chamber stores disinfectant hemostatic gel. While sampling, the gel pumping mechanism pumps the disinfectant hemostatic gel in the gel storage chamber into the gel spreading groove, completely covering the needle insertion site. After the needle is withdrawn, it can quickly cover the wound, stop bleeding, and disinfect, making hemostasis and disinfection more convenient. Attached Figure Description

[0020] Figure 1 This is a schematic diagram of the external structure of the present invention;

[0021] Figure 2 This is a schematic cross-sectional view of the present invention. Figure 1 ;

[0022] Figure 3 This is a schematic cross-sectional view of the present invention. Figure 2 ;

[0023] Figure 4 For the present invention Figure 3 Enlarged structural diagram at point A in the middle;

[0024] Figure 5 For the present invention Figure 3 Enlarged structural diagram at point B;

[0025] Figure 6This is a schematic diagram of the adjustable syringe holder and sampling needle structure of the present invention;

[0026] Figure 7 This is a schematic diagram of the adjustable syringe holder and sampling needle installation structure of the present invention.

[0027] Labels in the diagram: 1. Cell sampling pen; 2. Sampling cannula; 3. Pressure spring; 4. Adjustable syringe holder; 401. Fixing block; 402. Sector-shaped fixing groove; 403. Sampling slider; 5. Sampling needle; 501. Needle piston; 502. Piston rod; 503. Anti-rotation limiting plate; 504. Sector-shaped fixing block; 6. Needle mounting structure; 601. First sector-shaped locking block; 602. Second sector-shaped locking block; 7. Adjustment and positioning mechanism; 701. First adjustment groove 702. Second adjustment groove; 703. Adjustment slider; 704. Telescopic rod; 705. Telescopic groove; 706. Positioning spring; 707. Slider positioning tooth; 708. Positioning rack; 8. Needle protrusion hole; 9. Gel storage chamber; 10. Threaded cap; 11. Gel pumping mechanism; 1101. Gel pumping tube; 1102. Gel pushing piston; 1103. First one-way valve; 1104. Piston tube; 1105. Second one-way valve; 1106. Gel spreading groove. Detailed Implementation

[0028] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the scope of protection of the present invention.

[0029] To further understand the content of this invention, a detailed description of the invention will be provided in conjunction with the accompanying drawings.

[0030] Combination Figure 1 - Figure 7 A cell sampling device and its manufacturing method are disclosed, comprising a cell sampling pen 1, characterized in that: a sampling sleeve 2 is sleeved at one end of the cell sampling pen 1, a pressure spring 3 is provided at the right end of the sampling sleeve 2, an adjustable syringe holder 4 is provided inside the cell sampling pen 1, a sampling needle tube 5 is engaged with the adjustable syringe holder 4 through a needle tube mounting structure 6, an adjustment positioning mechanism 7 is provided at the right end of the adjustable syringe holder 4, a needle tube extension hole 8 is provided at the left end of the cell sampling pen 1, a gel storage chamber 9 is provided inside the cell sampling pen 1, the gel storage chamber 9 stores disinfectant hemostatic gel, a threaded cap 10 is threadedly connected to the right end of the cell sampling pen 1, and a gel pumping mechanism 11 is provided at the left end of the gel storage chamber 9.

[0031] Please see Figure 1 and Figure 2The adjustable syringe holder 4, sampling needle tube 5, and needle tube extension hole 8 are each provided with six sets, and the six sets of adjustable syringe holder 4, sampling needle tube 5, and needle tube extension hole 8 are spaced 60° apart.

[0032] The cells are evenly distributed at the angles, allowing for the completion of cell sampling at multiple different depths of the cortex in a single sampling operation.

[0033] Please see Figure 2 , Figure 4 and Figure 6 The adjusting positioning mechanism 7 includes a first adjusting groove 701. Six sets of parallel first adjusting grooves 701 are formed on the outer wall of the cell sampling pen 1. Six sets of parallel second adjusting grooves 702 are formed on the sampling sleeve 2 at positions corresponding to the first adjusting grooves 701. An adjusting slider 703 is movably mounted on the right end of the adjustable syringe holder 4. The adjusting slider 703 passes through the first adjusting groove 701 and the second adjusting groove 702. A telescopic rod 704 is provided at one end of the adjusting slider 703. A telescopic groove 705 is formed on the right end of the adjustable syringe holder 4. The telescopic rod 704 is inserted into the telescopic groove 705. A positioning spring 706 is provided inside the telescopic groove 705. Slider positioning teeth 707 are machined on both sides of the adjusting slider 703. Positioning racks 707 are machined on both sides of the first adjusting groove 701. 08. The slider positioning teeth 707 and the positioning rack 708 mesh with each other, so that the adjusting slider 703 can be pressed down, causing the slider positioning teeth 707 on the adjusting slider 703 to disengage from the positioning rack 708 on the first adjusting groove 701. Then, the adjusting slider 703 can be pushed left and right, thereby driving the sampling needle tube 5 to move left and right, thereby adjusting the length of the needle tip extension. This allows adjustment of the sampling depth of each sampling needle tube 5. After adjustment, the positioning spring 706 pushes the adjusting slider 703 upward, thereby causing the slider positioning teeth 707 and the positioning rack 708 to mesh again. Then, pushing the sampling slider 403 again can drive the needle tube piston 501 to move inside the sampling needle tube 5, thereby adjusting the stroke of the needle tube piston 501 and thus adjusting the sampling volume.

[0034] Please see Figure 4 The width of the slider positioning tooth 707 is less than the compression stroke of the telescopic rod 704, so that when the slider 703 is pressed down, the slider positioning tooth 707 can completely disengage from the positioning rack 708, which facilitates the movement of the slider 703.

[0035] Please see Figure 7The needle mounting structure 6 includes a first sector-shaped locking block 601 and a second sector-shaped locking block 602. The first sector-shaped locking block 601 is machined on the inner side of the tail of the sampling needle tube 5, and the second sector-shaped locking block 602 is machined on the left end of the adjustable syringe holder 4. The second sector-shaped locking block 602 is composed of two sets of centrally symmetrical 90° sector-shaped locking blocks, and the first sector-shaped locking block 601 is composed of two sets of centrally symmetrical 60° sector-shaped locking blocks. During installation, the sampling needle tube 5 is inserted into the needle tube extension hole 8, and the first sector-shaped locking block 601 on the right end of the sampling needle tube 5 and the second sector-shaped locking block 602 on the left end of the adjustable syringe holder 4 are engaged with each other. At the same time, the sector-shaped fixing block 504 passes through the sector-shaped fixing groove 402 on the fixing block 401. Then, the sampling needle tube 5 is rotated 90°, so that the first sector-shaped locking block 601 and the second sector-shaped locking block 602 are engaged with each other, thereby fixing the sampling needle tube 5 on the adjustable syringe holder 4.

[0036] Please see Figure 6 and Figure 7 The sampling needle tube 5 is equipped with a needle tube piston 501. One end of the needle tube piston 501 is fixedly connected to a piston rod 502. One end of the piston rod 502 is fixedly connected to a fan-shaped fixing block 504. A fixing block 401 is engaged in the arc-shaped groove on the adjustable syringe holder 4. The fixing block 401 has a fan-shaped fixing groove 402 that engages with the fan-shaped fixing block 504. A sampling slider 403 is fixedly connected to one side of the fixing block 401. The sampling slider 403 passes through the first adjustment groove 701 and the second adjustment groove 702. When the sampling tube 2 moves to the right to a certain position during sampling, the left end of the second adjustment groove 702 will drive the sampling slider 403 to move to the right. The sampling slider 403 can drive the needle tube piston 501 to move to the right, thereby realizing cell sampling by aspiration through the sampling needle tube 5.

[0037] Please see Figure 6 and Figure 7 The piston rod 502 has a cross-shaped cross section. The sampling needle tube 5 is provided with an anti-rotation limiting plate 503 at the right end. The piston rod 502 passes through the anti-rotation limiting plate 503, so that the sampling needle tube 5 can drive the piston rod 502 and the fan-shaped fixing block 504 to rotate together. In this way, the fan-shaped fixing block 504 is also engaged and fixed with the fixing block 401.

[0038] Please see Figure 5The gel pumping mechanism 11 includes a gel pumping tube 1101. The gel pumping tube 1101 is connected to the left end of the gel storage chamber 9. A gel-pushing piston 1102 is connected to the left end of the gel pumping tube 1101. A first one-way valve 1103 is installed inside the gel-pushing piston 1102. A piston tube 1104 is connected to the inner side of the left end of the sampling sleeve 2. The gel-pushing piston 1102 is located inside the piston tube 1104. A second one-way valve 1105 is installed at the left end of the piston tube 1104. The first one-way valve 1103 has a flow direction from right to left, and the second one-way valve 1105 has a flow direction from right to left. A gel spreading groove 1106 is provided at the left end outlet of the piston tube 1104. This allows the gel pumping mechanism 11 to pump the sterile hemostatic gel from the gel storage chamber 9 into the gel spreading groove 1106 simultaneously with the sampling needle tube 5. When the sampling cannula 2 rebounds to the left, the piston tube 1104 moves to the left along with the sampling cannula 2, increasing the space inside the piston tube 1104 and reducing the pressure. At this time, the second one-way valve 1105 closes, and the first one-way valve 1103 opens, allowing the sterile hemostatic gel from the gel storage chamber 9 to be drawn into the piston tube under pressure.

[0039] Inside 1104, when the sampling cannula 2 is pushed to the right, the first one-way valve 1103 closes and the second one-way valve 1105 opens, so that the disinfectant hemostatic gel is pumped into the gel spreading groove 1106, and then the entire piece of skin at the sampling site is wrapped in it. As the needle is pushed out, the disinfectant hemostatic gel with a certain degree of fluidity quickly covers the wound and forms a protective film for disinfection and hemostasis.

[0040] Please see Figure 2 and Figure 3 If the threaded cap 10 and the cell sampling pen 1 have low air tightness or the threaded cap 10 has ventilation during construction, the air pressure in the gel storage chamber 9 will be consistent with the atmospheric pressure when the piston tube 1104 moves to the left along with the sampling sleeve 2, so that the disinfectant hemostatic gel can be pumped into the piston tube 1104.

[0041] The manufacturing method includes the following steps:

[0042] S1. Aseptic manufacturing: First, the components of the sampling device are processed. After production, dust removal, cleaning and sterilization are carried out. Then, the parts are assembled in an aseptic environment.

[0043] S2. Production of disinfectant hemostatic gel: Fibrin monomers and neutralization buffer are reacted to prepare fibrin polymer. Neutralization buffer is added at a 1:1 ratio. Then, 1%-5% glycerol is added to the fibrin polymer to adjust the consistency. After that, bio-glue is added at a 1:1 ratio to form a hemostatic gel. Finally, 10% antibiotics are added to the hemostatic gel.

[0044] S3. Gel filling: Under aseptic conditions, the disinfectant hemostatic gel produced in step 2 is filled into the sampling device produced in step 1, and then aseptically packaged.

[0045] Working principle: When using a cell sampling device, first install the sampling needle tube 5 on the cell sampling pen 1. During installation, insert the sampling needle tube 5 into the needle tube extension hole 8, so that the first sector-shaped locking block 601 at the right end of the sampling needle tube 5 engages with the second sector-shaped locking block 602 at the left end of the adjustable syringe holder 4. At the same time, let the sector-shaped fixing block 504 pass through the sector-shaped fixing groove 402 on the fixing block 401. Then rotate the sampling needle tube 5 by 90°, so that the first sector-shaped locking block 601 and the second sector-shaped locking block 602 engage with each other, thereby fixing the sampling needle tube 5 on the adjustable syringe holder 4. While the sampling needle tube 5 rotates, since the piston rod 502 with a cross-shaped cross section engages with the anti-rotation limiting plate 503, the sampling needle tube 5 can drive the piston rod 502 and the sector-shaped fixing block 504 to rotate together. In this way, the sector-shaped fixing block 504 also engages with and is fixed to the fixing block 401.

[0046] Then, press down the adjusting slider 703 to disengage the slider positioning teeth 707 on the adjusting slider 703 from the positioning rack 708 on the first adjusting groove 701. Then, push the adjusting slider 703 left and right to move the sampling needle tube 5 left and right, thereby adjusting the length of the needle tip extension. This allows adjustment of the sampling depth of each sampling needle tube 5. After adjustment, the positioning spring 706 pushes the adjusting slider 703 upward, thereby re-engaging the slider positioning teeth 707 with the positioning rack 708. Then, push the sampling slider 403 to move the needle tube piston 501 inside the sampling needle tube 5, thereby adjusting the stroke of the needle tube piston 501 and thus adjusting the sampling volume.

[0047] During sampling, the left end of the cell sampling pen 1 is pressed against the sampling site, the pressure spring 3 is compressed, the sampling sleeve 2 moves to the right, and the needle of the sampling needle tube 5 extends out of the needle tube extension hole 8 and is inserted subcutaneously for sampling. When the second adjustment groove 702 on the sampling sleeve 2 moves to a certain position to the right, the left end of the second adjustment groove 702 will drive the sampling slider 403 to move to the right, thereby driving the needle tube piston 501 to move to the right, so as to realize cell sampling by aspiration through the sampling needle tube 5. After sampling, the sampling needle tube 5 can be removed to collect and process the sample, and a new sampling needle tube 5 can be replaced.

[0048] While the sampling needle 5 is taking samples, the gel pumping mechanism 11 pumps the disinfectant hemostatic gel in the gel storage chamber 9 into the gel spreading groove 1106. When the sampling cannula 2 rebounds to the left, the piston tube 1104 moves to the left along with the sampling cannula 2, thereby increasing the space and reducing the pressure inside the piston tube 1104. At this time, the second one-way valve 1105 closes and the first one-way valve 1103 opens, so the disinfectant hemostatic gel in the gel storage chamber 9 is drawn into the piston tube 1104 under pressure. When the sampling cannula 2 is pushed to the right, the first one-way valve 1103 closes and the second one-way valve 1105 opens, so the disinfectant hemostatic gel is pumped into the gel spreading groove 1106, and then the entire piece of skin at the sampling site is wrapped in it. As the needle is pushed out, the disinfectant hemostatic gel with a certain degree of fluidity quickly covers the wound and forms a protective film for disinfection and hemostasis.

[0049] Although embodiments of the invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A cell sampling device, comprising a cell sampling pen (1), characterized in that: The cell sampling pen (1) is fitted with a sampling sleeve (2) at one end. A pressure spring (3) is provided at the right end of the sampling sleeve (2). An adjustable syringe holder (4) is provided inside the cell sampling pen (1). A sampling needle tube (5) is connected to the adjustable syringe holder (4) by a needle tube mounting structure (6). An adjustment positioning mechanism (7) is provided at the right end of the adjustable syringe holder (4). A needle tube extension hole (8) is provided at the left end of the cell sampling pen (1). A gel storage chamber (9) is provided inside the cell sampling pen (1). The gel storage chamber (9) stores disinfectant hemostatic gel. A threaded cap (10) is connected to the right end of the cell sampling pen (1) by a thread. A gel pumping mechanism (11) is provided at the left end of the gel storage chamber (9). The adjustment and positioning mechanism (7) includes a first adjustment groove (701). Six sets of parallel first adjustment grooves (701) are provided on the outer wall of the cell sampling pen (1). Six sets of parallel second adjustment grooves (702) are provided on the sampling sleeve (2) corresponding to the first adjustment grooves (701). An adjustment slider (703) is movably mounted on the right end of the adjustable syringe holder (4). The adjustment slider (703) passes through the first adjustment groove (701) and the second adjustment grooves (702). A telescopic rod (704) is provided at one end of the block (703), and a telescopic groove (705) is provided at the right end of the adjustable syringe holder (4). The telescopic rod (704) is inserted into the telescopic groove (705), and a positioning spring (706) is provided in the telescopic groove (705). Slider positioning teeth (707) are machined on both sides of the adjusting slider (703), and positioning racks (708) are machined on both sides of the first adjusting groove (701). The slider positioning teeth (707) and the positioning racks (708) mesh with each other. The sampling needle tube (5) is provided with a needle tube piston (501). One end of the needle tube piston (501) is fixedly connected to a piston rod (502). One end of the piston rod (502) is fixedly connected to a fan-shaped fixing block (504). A fixing block (401) is engaged in the arc groove on the adjustable syringe holder (4). A fan-shaped fixing groove (402) is opened in the fixing block (401) to engage with the fan-shaped fixing block (504). A sampling slider (403) is fixedly connected to one side of the fixing block (401). The sampling slider (403) passes through the first adjustment groove (701) and the second adjustment groove (702).

2. The cell sampling device according to claim 1, characterized in that: The adjustable syringe holder (4), sampling needle (5) and needle extension hole (8) are provided in six sets, and the six sets of adjustable syringe holder (4), sampling needle (5) and needle extension hole (8) are evenly distributed at a 60° interval.

3. The cell sampling device according to claim 1, characterized in that: The width of the slider positioning tooth (707) is less than the compression stroke of the telescopic rod (704).

4. The cell sampling device according to claim 1, characterized in that: The needle mounting structure (6) includes a first sector-shaped locking block (601) and a second sector-shaped locking block (602). The sampling needle tube (5) has a first sector-shaped locking block (601) machined on the inner side of its tail. The adjustable syringe holder (4) has a second sector-shaped locking block (602) machined on its left end. The second sector-shaped locking block (602) is composed of two sets of 90° angle sector-shaped locking blocks that are centrally symmetrical. The first sector-shaped locking block (601) is composed of two sets of 60° angle sector-shaped locking blocks that are centrally symmetrical.

5. A cell sampling device according to claim 1, characterized in that: The piston rod (502) has a cross-shaped cross section, and the sampling needle tube (5) is provided with an anti-rotation limiting plate (503) at the right end. The piston rod (502) passes through the anti-rotation limiting plate (503).

6. A cell sampling device according to claim 1, characterized in that: The gel pumping mechanism (11) includes a gel pumping tube (1101), the gel storage chamber (9) is connected to the left end of the gel pumping tube (1101), the gel pumping tube (1101) is connected to the left end of the gel pumping tube (1101) and a gel pushing piston (1102) is provided inside the gel pushing piston (1102). A first one-way valve (1103) is provided inside the piston tube (1103) on the left side of the sampling sleeve (2). The gel pushing piston (1102) is located inside the piston tube (1104). A second one-way valve (1105) is provided at the left end of the piston tube (1104). The first one-way valve (1103) is in the direction of passage from right to left, and the second one-way valve (1105) is in the direction of passage from right to left. A gel spreading groove (1106) is provided at the outlet of the left end of the piston tube (1104).

7. A cell sampling device according to claim 1, characterized in that: The threaded cap (10) has low airtightness with the cell sampling pen (1) or the threaded cap (10) is ventilated during construction.

8. A method for manufacturing a cell sampling device according to any one of claims 1-7, characterized in that: Includes the following steps: S1. Aseptic manufacturing: First, the components of the sampling device are processed. After production, dust removal, cleaning and sterilization are carried out. Then, the parts are assembled in an aseptic environment. S2. Production of disinfectant hemostatic gel: Fibrin monomers and neutralization buffer are reacted to prepare fibrin polymer. Neutralization buffer is added at a 1:1 ratio. Then, 1%-5% glycerol is added to the fibrin polymer to adjust the consistency. After that, bio-glue is added at a 1:1 ratio to form a hemostatic gel. Finally, 10% antibiotics are added to the hemostatic gel. S3. Gel filling: Under aseptic conditions, the disinfectant hemostatic gel produced in step 2 is filled into the sampling device produced in step 1, and then aseptically packaged.

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