Cryopreservation biological sample bank device and access method

Abstract

The application discloses a kind of cryogenic biological sample bank equipment and access method, including frame platform, storage mechanism, transfer mechanism, freezing tank, clamping mechanism, tray access mechanism and pick-up mechanism;Frame platform includes outer frame and middle layer platform;Middle layer platform divides outer frame into upper space and lower space;Storage mechanism is arranged in lower space, and built-in basket frame is arranged;Middle layer platform is provided with first through hole and second through hole;The lower end of transfer mechanism is located in lower space;Freezing tank is installed in second through hole;Clamping mechanism, tray access mechanism and pick-up mechanism are all arranged in upper space;Tray access mechanism is located below clamping mechanism;Pick-up mechanism is located above freezing tank.The application can complete the function of automatic access of cryopreservation box by the cooperation of the above device, thereby effectively reducing the interference of human factors, improving the safety, reliability and convenience of access, and effectively improving the protection effect of biological sample.

Description

TECHNICAL FIELD

[0001] The present application relates to the field of biological sample library, in particular to a deep low-temperature biological sample library device and access method. BACKGROUND

[0002] Long-term storage of biological samples usually uses as low a temperature as possible to reduce biochemical reactions within the sample and improve the stability of various components within the sample. The lower the temperature, the longer the sample can be stored. Typically, cryopreserved cells are placed in test tubes, the test tubes are placed in sample boxes, and the sample boxes are placed on storage racks, which are placed in a refrigerated environment to achieve long-term stable storage of the samples.

[0003] However, the existing biological sample library has certain defects. For example, in the prior art, human intervention is required during the storage and retrieval of biological samples. Errors in placement and retrieval can occur during the above process, which is not conducive to the user's quick search and placement, and human intervention can damage the overall refrigerated environment, thereby causing the biological samples to become inactive due to temperature changes.

[0004] Therefore, there is a need for a deep low-temperature biological sample library device and access method to solve the above problems. SUMMARY

[0005] The present application aims to provide a deep low-temperature biological sample library device and access method to reduce human interference by automating the storage and retrieval of biological samples, improve the safety, reliability and convenience of storage and retrieval, and effectively improve the protection effect of biological samples.

[0006] To solve the above technical problems, the present application provides a deep low-temperature biological sample library device, comprising a frame platform, a storage mechanism, a transfer mechanism, a freezing tank, a clamping mechanism, a tray in-out mechanism and a picking mechanism.

[0007] The frame platform comprises an outer frame and a middle platform fixed to the outer frame, the middle platform is horizontally arranged, and the space defined by the outer frame is divided into an upper space and a lower space;

[0008] The storage mechanism is arranged in the lower space and has a basket rack for storing cryopreservation boxes built-in;

[0009] The middle platform is provided with a first through hole and a second through hole;

[0010] The lower end of the transfer mechanism is located in the lower space, and the upper end of the transfer mechanism moves up and down between the upper space and the lower space through the first through hole;

[0011] The freezing tank is installed in the second through hole;

[0012] The clamping mechanism, the tray in-out mechanism and the picking mechanism are arranged in the upper space, the clamping mechanism can move between the storage mechanism, the transfer mechanism, the tray in-out mechanism and the freezing tank and realize clamping of the basket frame or the cryogenic box;

[0013] The tray in-out mechanism is below the clamping mechanism, can move between the storage mechanism and the transfer mechanism and carry the cryogenic box;

[0014] The picking mechanism is above the freezing tank, can take out the target cryogenic tube from a cryogenic box and place the target cryogenic tube in another cryogenic box.

[0015] Further, the upper space is further provided with a heat preservation shell, the heat preservation shell is connected with the middle platform to form a closed chamber, and the closed chamber maintains a micro-positive pressure state.

[0016] Further, the storage mechanism includes a tank body, an inner tank and a heat preservation cover;

[0017] The tank body is fixedly installed in the lower space, and the top edge of the tank body is provided with an annular flange, and the top of the tank body is sealingly connected with the middle platform through the annular flange;

[0018] The heat preservation cover is arranged on the upper surface of the tank body, the middle platform is provided with a third through hole, and the position of the third through hole corresponds to the heat preservation cover;

[0019] The inner tank is rotatably installed in the tank body by a driving mechanism;

[0020] The basket frame is a plurality of, and is arranged in a ring array with the center axis of the inner tank as the array center;

[0021] The driving mechanism can rotate the target basket frame to below the heat preservation cover.

[0022] Further, the middle platform is provided with a ventilation ring and a hot air fan;

[0023] The ventilation ring is sleeved on the center axis of the inner tank, and the hot air fan is connected with the ventilation ring through a pipeline.

[0024] Further, the clamping mechanism includes a three-axis displacement table, a basket module and a cover lifting module;

[0025] The cover lifting module is fixed on the X-axis of the three-axis displacement table, and is used for opening the heat preservation cover to expose the basket frame;

[0026] The basket module has a picking end and a connecting end, the connecting end is installed on the Z-axis of the three-axis displacement table, and the picking end is driven to realize vertical movement and horizontal movement;

[0027] The picking end has two oppositely arranged picking clamps, and the two picking clamps can approach or move away from each other.

[0028] Further, the picking end is wrapped with a cold shield, the structure size of the cold shield is set according to the outer size of the basket frame, and a gap is reserved between the bottom end of the cold shield and the tank body.

[0029] Further, the tray in-out mechanism includes a sliding rail and a tray carrier plate;

[0030] The sliding rail is installed on the middle layer platform, and the tray carrier plate is slidingly installed on the sliding rail;

[0031] When the clamping mechanism clamps the basket frame out of the storage mechanism, the tray carrier plate slides into the basket frame, and the clamping mechanism and the tray in-out mechanism cooperate to transfer the cryopreservation box in the basket frame to the tray carrier plate, or transfer the cryopreservation box on the tray carrier plate to the basket frame.

[0032] Further, the transfer mechanism includes a lifting device, a transfer tank and a position sensor;

[0033] The lifting device is arranged in the lower space;

[0034] The transfer tank is installed on the lifting device;

[0035] The position sensor is arranged on the lifting device to detect whether the transfer tank is lifted into the upper space;

[0036] A sealing ring is arranged on the middle layer platform, the sealing ring surrounds the first through hole, and when the transfer tank moves to the upper space through the first through hole, the sealing ring is compressed to realize sealed docking with the middle layer platform.

[0037] Further, the frame platform is built-in a code scanner, the code scanner is located between the transfer mechanism and the tray in-out mechanism, and is used for identifying the cryopreservation box.

[0038] Further, the picking and clamping mechanism includes a clamping module and a tube picking needle module;

[0039] The pair of clamping modules and the tube picking and ejecting module are arranged above the freezing tank, the pair of clamping modules are used for positioning the first cryopreservation box and the second cryopreservation box in the freezing tank, and the tube picking and ejecting module is used for picking a target cryopreservation tube in the first cryopreservation box and placing the target cryopreservation tube in the second cryopreservation box.

[0040] In further embodiments, the application also provides a method for accessing a cryopreserved biological sample, which is used for accessing a cryopreservation box in a cryopreserved biological sample bank device, and the method comprises the following steps:

[0041] The transfer mechanism is driven to ascend, so that the first cryopreservation box in the transfer mechanism is transferred from the lower space to the upper sealed chamber;

[0042] The first cryopreservation box is transferred from the transfer mechanism to the tray in-out mechanism by the clamping mechanism;

[0043] The basket frame in the storage mechanism is clamped by the clamping mechanism;

[0044] The tray in-out mechanism is driven to move, and the first cryopreservation box is transferred to the basket frame by the clamping mechanism;

[0045] The basket frame is placed in the storage mechanism by the clamping mechanism, so as to complete the storage of the first cryopreservation box;

[0046] The basket frame in the storage mechanism is clamped by the clamping mechanism;

[0047] The tray in-out mechanism is driven to move, and the first cryopreservation box in the basket frame is transferred to the tray in-out mechanism by the clamping mechanism;

[0048] The first cryopreservation box on the tray in-out mechanism is clamped to the freezing tank by the clamping mechanism;

[0049] The target cryopreservation tube in the first cryopreservation box is clamped to the second cryopreservation box by the clamping mechanism;

[0050] The second cryopreservation box containing the target cryopreservation tube is clamped to the transfer mechanism by the clamping mechanism;

[0051] The transfer mechanism is driven to descend, so that the second cryopreservation box and the target cryopreservation tube are transferred from the upper sealed chamber to the lower space, so as to complete the extraction of the second cryopreservation box and the target cryopreservation tube.

[0052] Compared with the prior art, the application has at least the following beneficial effects:

[0053] When the cryopreservation box needs to be stored, the transfer mechanism is used for conveying, the tray in-out mechanism is used for carrying, and the clamping mechanism is used for clamping the basket frame to complete the storage of the cryopreservation box. When the cryopreservation tube in the cryopreservation box needs to be taken out, the clamping mechanism is used for clamping the basket frame, the tray in-out mechanism is used for carrying, the picking mechanism is used for picking, and the transfer mechanism is used for outputting to complete the taking of the cryopreservation tube, thereby realizing the automatic storage and taking of the cryopreservation box, effectively reducing the interference of human factors, improving the safety, reliability and convenience of storage and taking, and effectively improving the protection effect of the biological sample.

[0054] Further, by setting the heat preservation cover, the cold cover and the rubber ring, and integrating the components in the same frame platform, the cryopreservation box is always in a stable refrigeration environment during transportation, conveying and storage, thereby effectively avoiding the inactivation of the biological sample in the cryopreservation box due to excessive temperature difference, further improving the protection effect of the biological sample, and reducing the consumption of refrigerant and the cost of cryopreservation box storage and taking.

[0055] In addition, the code scanner is arranged to identify the cryopreservation box, thereby improving the accuracy of storage and taking, avoiding the wrong storage and taking, and realizing the automatic data management of the biological sample information. BRIEF DESCRIPTION OF DRAWINGS

[0056] Figure 1 It is a structure schematic view of the cryopreservation biological sample library in an embodiment of the present application.

[0057] Figure 2 It is a structure schematic view of the cryopreservation biological sample library in an embodiment of the present application.

[0058] Figure 3 It is a structure schematic view of the storage mechanism in the cryopreservation biological sample library in an embodiment of the present application.

[0059] Figure 4 It is a structure sectional view of the storage mechanism in the cryopreservation biological sample library in an embodiment of the present application.

[0060] Figure 5 It is a structure schematic view of the driving mechanism in the cryopreservation biological sample library in an embodiment of the present application.

[0061] Figure 6 It is a structure schematic view of the basket frame in the cryopreservation biological sample library in an embodiment of the present application.

[0062] Figure 7 It is a structure schematic view of the middle layer platform in the cryopreservation biological sample library in an embodiment of the present application.

[0063] Figure 8 Figure 1 is a structural schematic diagram of a tray access mechanism in a cryogenic biological sample bank according to an embodiment of the present application;

[0064] Figure 9 Figure 2 is a structural schematic diagram of a gripper mechanism in a cryogenic biological sample bank according to an embodiment of the present application;

[0065] Figure 10 Figure 3 is a partial structural schematic diagram of a basket module in a cryogenic biological sample bank according to an embodiment of the present application;

[0066] Figure 11 Figure 4 is a structural schematic diagram of a transfer mechanism in a cryogenic biological sample bank according to an embodiment of the present application;

[0067] Figure 12 Figure 5 is a structural schematic diagram of a code scanner in a cryogenic biological sample bank according to an embodiment of the present application;

[0068] Figure 13 Figure 6 is a structural schematic diagram of a freezing tank in a cryogenic biological sample bank according to an embodiment of the present application;

[0069] Figure 14 Figure 7 is a structural schematic diagram of a pick-and-grip mechanism in a cryogenic biological sample bank according to an embodiment of the present application;

[0070] Figure 15 Figure 8 is a flowchart of a cryogenic biological sample bank storage and retrieval method according to another embodiment of the present application.

[0071] Figure 1 is a structural schematic diagram of a tray access mechanism in a cryogenic biological sample bank according to an embodiment of the present application; Figure 2 is a structural schematic diagram of a gripper mechanism in a cryogenic biological sample bank according to an embodiment of the present application; Figure 3 is a partial structural schematic diagram of a basket module in a cryogenic biological sample bank according to an embodiment of the present application; Figure 4 is a structural schematic diagram of a transfer mechanism in a cryogenic biological sample bank according to an embodiment of the present application; Figure 5 is a structural schematic diagram of a code scanner in a cryogenic biological sample bank according to an embodiment of the present application; Figure 6 is a structural schematic diagram of a freezing tank in a cryogenic biological sample bank according to an embodiment of the present application; Figure 7 is a structural schematic diagram of a pick-and-grip mechanism in a cryogenic biological sample bank according to an embodiment of the present application; Figure 8 is a flowchart of a cryogenic biological sample bank storage and retrieval method according to another embodiment of the present application; Figure 9 is a structural schematic diagram of a cryogenic biological sample bank according to an embodiment of the present application; and Figure 10 is a structural schematic diagram of a cryogenic biological sample bank according to another embodiment of the present application. DETAILED DESCRIPTION

[0072] The cryogenic biological sample bank apparatus and retrieval method of the present application will be described in greater detail below with reference to the accompanying drawings, in which the preferred embodiments of the present application are illustrated. It should be understood that those skilled in the art can modify the present application described herein while still achieving the advantageous effects of the present application. Accordingly, the following description should be understood as a broad teaching to those skilled in the art, and not as a limitation on the present application.

[0073] The invention is described more specifically by way of example in the following paragraphs with reference to the accompanying drawings. The advantages and features of the invention will become clearer from the following description. It should be noted that the drawings are in a very simplified form and use non-precise proportions, and are only used to facilitate and clarify the illustration of the embodiments of the invention.

[0074] Example 1

[0075] like Figures 1 to 7 As shown in the figure, an embodiment of the present invention proposes a cryogenic biological sample bank, including a frame platform 1, a storage mechanism 2, a transfer mechanism 3, a freezing tank 5, a clamping mechanism 7, a tray entry and exit mechanism 4, and a picking and clamping mechanism 6.

[0076] The frame platform 1 includes an outer frame and a middle platform 11 fixed to the outer frame. The middle platform 11 is arranged horizontally, dividing the space defined by the outer frame into an upper space and a lower space.

[0077] like Figure 1 , Figure 2 and Figure 4 As shown, the storage mechanism 2 is located in the lower space and has a built-in basket rack 8 for storing cryopreservation boxes.

[0078] like Figure 6 As shown, the middle platform 11 has a first through hole 111 and a second through hole 112.

[0079] The lower end of the transfer mechanism 3 is located in the lower space, and the upper end of the transfer mechanism 3 moves up and down between the upper space and the lower space through the first through hole 111. That is, the input or output of the cryopreservation box is completed by moving up and down between the upper space and the lower space.

[0080] The freezing tank 5 is installed in the second through hole 112 to accommodate the transferred cryopreservation box.

[0081] The clamping mechanism 7, the tray entry / exit mechanism 4, and the picking and clamping mechanism 6 are all located in the upper space. The clamping mechanism 7 can move between the storage mechanism 2, the transfer mechanism 3, the tray entry / exit mechanism 4, and the freezing tank 5 to clamp the basket rack 8 or the cryopreservation box.

[0082] The tray in / out mechanism 4 is located below the clamping mechanism 7 and can move back and forth between the storage mechanism 2 and the transfer mechanism 3 to carry the cryopreservation box.

[0083] The picking and clamping mechanism 6 is located above the freezing tank 5 and can remove the target cryopreservation tube from one cryopreservation box and place the target cryopreservation tube into another cryopreservation box (e.g., an empty cryopreservation box).

[0084] In summary, the device is provided with a storage mechanism 2, a transfer mechanism 3, a tray in-out mechanism 4, a freezing tank 5, a picking mechanism 6 and a clamping mechanism 7.

[0085] When the cryotubes need to be stored, the cryotubes to be stored are transferred to the upper space by the transfer mechanism 3, and then placed on the tray in-out mechanism 4 under the clamping of the clamping mechanism 7. Then the basket frame 8 is taken out of the storage mechanism 2 by the clamping mechanism 7, and the cryotubes on the tray in-out mechanism 4 are transferred to the designated position in the basket frame 8. Finally, the basket frame 8 is placed in the storage mechanism 2 by the clamping mechanism 7, and the storage of the cryotubes is completed.

[0086] When the cryotubes need to be stored, the cryotubes to be stored are transferred to the upper space by the transfer mechanism 3, and then placed on the tray in-out mechanism 4 under the clamping of the clamping mechanism 7. Then the basket frame 8 is taken out of the storage mechanism 2 by the clamping mechanism 7, and the cryotubes on the tray in-out mechanism 4 are transferred to the designated position in the basket frame 8. Finally, the basket frame 8 is placed in the storage mechanism 2 by the clamping mechanism 7, and the storage of the cryotubes is completed.

[0087] Thus, automatic storage and retrieval are realized, and the interference of human factors is reduced. Compared with the existing technology, the device can improve the safety, reliability and convenience of storage and retrieval, and effectively improve the protection effect of biological samples.

[0088] As shown in Figure 1 , Figure 2 and Figure 7 , in the present embodiment, an insulation shell 12 is further provided outside the upper space, and the insulation shell 12 is connected with the middle platform 11 to form a closed chamber.

[0089] By providing the insulation shell 12, the upper space is absolutely isolated from the outside, so that when the tray in-out mechanism 4, the picking mechanism 6 and the clamping mechanism 7 are working, the cryotubes will not be damaged due to large temperature difference.

[0090] The side of the upper space is further provided with a transparent observation window for the operator to check the running state of the entire device.

[0091] Please continue to refer to Figure 7 , the middle platform 11 is provided with a ventilation ring 9 and a hot air fan 10.

[0092] The ventilation ring 9 is sleeved on the central shaft of the storage mechanism 2, and the hot air machine 10 is connected with the ventilation ring 9 through a pipeline.

[0093] When the device is started for the first time, the air in the upper space can be quickly diluted through the volatilization of the refrigerant and the blowing of the hot air machine 10, so that the dew point of the entire upper space is lowered during further cooling, and the situation that the storage mechanism 2 cannot be opened due to frosting and icing is avoided, and the stability of the entire device operation is improved.

[0094] During normal operation of the device, when the upper space needs to be opened for maintenance due to an emergency, the storage mechanism 2 will freeze and dew due to the water vapor contained in the air, that is, the inside of the storage mechanism 2 will absorb heat and cool down, which will cause the biological samples to be stressed by the storage environment, increasing the risk of damage to the biological samples. However, by setting the ventilation ring 9 and the hot air machine 10, the storage mechanism 2 is warmed up to effectively prevent the biological samples in the storage mechanism 2 from dying due to rapid cooling, and the protection effect on the biological samples is improved.

[0095] In addition, it should be particularly pointed out that during operation, the closed chamber maintains a slightly positive pressure state, thereby ensuring that the dew point in the upper space is lower than the set value, to further avoid the situation that the storage mechanism 2 cools too quickly in an emergency, and further improve the protection effect on the biological samples.

[0096] Please continue to refer to Figure 3 , Figure 4 and Figure 5 In this embodiment, a specific storage mechanism 2 is also proposed to improve the storage effect of the cryopreservation box.

[0097] The storage mechanism 2 includes a tank body 21, an inner tank 22, and a heat preservation cover 23. The tank body 21 is fixedly installed in the lower space, and the top edge of the tank body 21 is provided with an annular flange (not numbered in the figure). The top of the tank body 21 is sealingly connected with the middle layer platform 11 through the annular flange.

[0098] It should be particularly pointed out that the top of the tank body 21 is an arc-shaped protruding structure, and a circular hole (not numbered in the figure) is formed on the middle layer platform 11 to avoid the protruding part of the top.

[0099] The heat preservation cover 23 is arranged on the upper surface of the tank body 21, the middle layer platform 11 is provided with a third through hole 113, and the position of the third through hole 113 corresponds to the heat preservation cover 23.

[0100] The inner tank 22 is installed in the tank body 21, and the central shaft of the inner tank 22 is connected with a driving mechanism 24. The driving mechanism 24 drives the central shaft of the inner tank 22 to rotate, so that the relative rotation of the inner tank 22 and the tank body 21 is realized.

[0101] The basket frames 8 are multiple, and the multiple basket frames 8 are vertically arranged and distributed in a ring array with the central axis of the inner tank 22 as the array center.

[0102] In this embodiment, in order to reduce the opening size while retaining the traditional sealing cover structure, a rectangular heat preservation cover 23 is arranged on the traditional circular cover, thereby reducing the influence of opening on the storage environment in the tank.

[0103] It should be particularly pointed out that the driving mechanism 24 can rotate the target basket frame 8 to the lower side of the heat preservation cover 23.

[0104] As shown in the figure, Figure 9 In this embodiment, a specific clamping mechanism 7 is also proposed to improve the clamping effect on the cryopreservation box and the basket frame 8.

[0105] The clamping mechanism 7 includes a three-axis displacement table, a basket module 71, and a cover opening module 72.

[0106] The cover opening module 72 is fixed on the X-axis of the three-axis displacement table and can move back and forth along the X-axis to open or close the heat preservation cover 23. When the heat preservation cover 23 is opened, the basket frame 8 is exposed.

[0107] The basket module 71 has a picking end and a connecting end, and the connecting end is installed on the Z-axis of the three-axis displacement table. Since the Z-axis of the three-axis displacement table is connected with the X-axis and can move back and forth along the X-axis, the picking end can be driven to move vertically (i.e., move back and forth along the Z-axis direction) and horizontally (i.e., move back and forth along the X-axis direction).

[0108] The picking end has two oppositely arranged picking clamps, and the two picking clamps can move closer to or away from each other, that is, the distance between the two picking clamps can be adjusted according to the different grasping objects (the basket frame 8 or the cryopreservation box).

[0109] In addition, the picking end is wrapped with a cold shield 73, and the structure and size of the cold shield 73 are set according to the outer shape and size of the basket frame 8. The picked basket frame 8 is placed in the cold shield 73, so that when the basket frame 8 is taken, the internal cryopreservation box can also be in a stable refrigeration environment, further improving the protection effect on biological samples.

[0110] It should be particularly pointed out that, in order to prevent the existence of the cold shield 73 from interfering with the operation of the tray in-out mechanism 4, the relationship between the two is further limited.

[0111] Specifically, a gap is reserved between the bottom end of the cold shield 73 and the tank body 21, and the tray access mechanism 4 can move into the basket frame 8 via the gap to take out or put in the cryo box.

[0112] As shown in Figure 2 and Figure 8 In the present embodiment, a specific tray access mechanism 4 is also proposed to cooperate with the clamping mechanism 7 to complete the taking out and putting in of the cryo box, which includes a slide rail 41 and a tray carrier 42. The slide rail 41 is installed on the middle layer platform 11, one end of the tray carrier 42 is installed on the slide rail 41, and the other end of the tray carrier 42 is used to carry the cryo box (not numbered in the figure). The tray carrier 42 can move back and forth along the slide rail 41 with the cryo box carried by it.

[0113] When the clamping mechanism 7 clamps the basket frame 8 out of the storage mechanism 2, the tray carrier 42 slides into the basket frame 8, and the clamping mechanism 7 and the tray access mechanism 4 cooperate to transfer the cryo box in the basket frame 8 to the tray carrier 42 or transfer the cryo box on the tray carrier 42 to the basket frame 8.

[0114] That is, when the tray carrier 42 slides into the basket frame 8, the basket frame 8 is lowered by the clamping mechanism 7, so that the cryo box above the tray carrier 42 is overlapped on the tray carrier 42, and the cryo box is separated from the placement cavity in the basket frame 8. Then, by moving the tray carrier 42 in the opposite direction, the taking out of the cryo box is completed. Conversely, by overlapping the placement cavity with the cryo box, the cryo box is separated from the tray carrier 42 to complete the putting in of the cryo box, which will not be described here.

[0115] As shown in Figure 11 In the present embodiment, a specific transfer mechanism 3 is also proposed to improve the stability of the input and output of the cryo box.

[0116] The transfer mechanism 3 includes a lifting device 31, a transfer tank 32, and a position sensor 33.

[0117] The lifting device 31 is arranged in the lower space, and the transfer tank 32 is installed on the lifting device 31.

[0118] The position sensor 33 is arranged on the lifting device 31 to detect whether the transfer tank 32 is lifted into the upper space.

[0119] The position of the transfer tank 32 is recognized by setting the position sensor 33, and then the position of the transfer tank 32 can be transmitted to the clamping mechanism 7 by the background control system, so as to take the cryopreservation box in the transfer tank 32 or place the empty cryopreservation box in the transfer tank 32, and achieve the functions of automatic input and output of the cryopreservation box.

[0120] It should be particularly pointed out that the middle layer platform 11 is provided with a sealing ring, which surrounds the first through hole 111. When the transfer tank 32 moves to the upper space through the first through hole 111, the sealing ring is extruded to realize the sealed docking with the middle layer platform 11.

[0121] As shown in Figure 2 and Figure 12 The frame platform 1 is provided with a code scanner 13, which is located between the transfer mechanism 3 and the tray in-out mechanism 4, so as to identify the cryopreservation box input by the transfer mechanism 3.

[0122] Specifically, the code scanner 13 includes a code scanner body and an assembly assembly. The code scanner body is fixed on the middle layer platform 11 through the assembly assembly, and the middle layer platform 11 is provided with a code scanning window. By identifying the input cryopreservation box and reflecting to the background control terminal, the automatic data management of the biological sample information is realized, so as to avoid the wrong storage and the wrong taking.

[0123] As shown in Figure 13 and Figure 14 In further embodiments, a specific picking and clamping mechanism 6 is also proposed to improve the picking and clamping effect of the cryopreservation tube.

[0124] The picking and clamping mechanism 6 includes a clamping module 61 and a tube picking needle module 62.

[0125] The clamping module 61 and the tube picking needle module 62 are arranged above the freezing tank 5. The clamping module 61 is used for positioning the first cryopreservation box and the second cryopreservation box in the freezing tank 5. The tube picking needle module 62 is used for picking the target cryopreservation tube in the first cryopreservation box and placing the target cryopreservation tube in the second cryopreservation box (i.e. the above-mentioned empty cryopreservation box, which is used for accommodating the target cryopreservation tube).

[0126] Embodiment two

[0127] As shown in Figure 15 In further embodiments, the present application also proposes a method for storing and taking deep cryogenic biological samples, which is used for storing and taking cryopreservation boxes in a deep cryogenic biological sample bank device. The method comprises the following steps:

[0128] S1, drive the middle transfer mechanism 3 to rise, so that the first cryopreservation box in the middle transfer mechanism 3 is transferred from the lower space to the upper closed chamber;

[0129] S2, transfer the first cryopreservation box from the middle transfer mechanism 3 to the tray access mechanism 4 by the clamping mechanism 7;

[0130] S3, clamp the basket frame 8 in the storage mechanism 2 by the clamping mechanism 7;

[0131] S4, drive the tray access mechanism 4 to move, cooperate with the clamping mechanism 7 to transfer the first cryopreservation box into the basket frame 8 (i.e. the tray access mechanism 4 moves to the position of the storage mechanism 2, the tray 42 is inserted into the basket frame 8, the cryopreservation box is placed in the designated position of the basket frame 8, and then the tray access mechanism 4 moves away from the basket frame 8);

[0132] S5, place the basket frame 8 in the storage mechanism 2 by the clamping mechanism 7 to complete the storage of the first cryopreservation box;

[0133] S6, clamp the basket frame 8 in the storage mechanism 2 by the clamping mechanism 7;

[0134] S7, drive the tray access mechanism 4 to move, cooperate with the clamping mechanism 7 to transfer the first cryopreservation box in the basket frame 8 to the tray access mechanism 4;

[0135] S8, clamp the first cryopreservation box on the tray access mechanism 4 to the cryopreservation slot by the clamping mechanism 7;

[0136] S9, clamp the target cryopreservation tube in the first cryopreservation box to the second cryopreservation box by the clamping mechanism 6;

[0137] S10, clamp the second cryopreservation box storing the target cryopreservation tube to the middle transfer mechanism 3 by the clamping mechanism 7;

[0138] S11, drive the middle transfer mechanism 3 to descend, transfer the second cryopreservation box and the target cryopreservation tube from the upper closed chamber to the lower space to complete the extraction of the second cryopreservation box and the target cryopreservation tube.

[0139] Wherein, the second cryopreservation box is an empty cryopreservation box, which is stored in the storage mechanism 2 and taken out from the storage mechanism 2 in advance when needed and placed in the cryopreservation slot, and the taking and placing process of the empty cryopreservation box is completed by the cooperation of the clamping mechanism 7 and the tray access mechanism 4, which is similar to the first cryopreservation box.

[0140] To sum up, the device can realize the automatic storage and taking of the cryopreservation tube and the cryopreservation box through the above steps, thereby effectively reducing the interference of human factors, improving the safety, reliability and convenience of storage and taking, and effectively improving the protection effect of biological samples.

[0141] Obviously, various modifications and changes can be made to the present application by those skilled in the art without departing from the spirit and scope of the present application. Thus, if these modifications and changes of the present application fall within the scope of the claims of the present application and their equivalents, it is intended to include these modifications and changes in the present application.

Claims

1. A cryogenic biobank device, characterized in that, include: Frame platform, storage mechanism, transfer mechanism, freezing tank, clamping mechanism, pallet in / out mechanism, and picking and clamping mechanism; The frame platform includes an outer frame and a middle platform fixed to the outer frame. The middle platform is arranged horizontally, dividing the space defined by the outer frame into an upper space and a lower space. The storage mechanism is located in the lower space and has a built-in basket rack for storing cryopreservation boxes. The middle platform is provided with a first through hole and a second through hole; The lower end of the transfer mechanism is located in the lower space, and the upper end of the transfer mechanism moves up and down between the upper space and the lower space through the first through hole. The freezing tank is installed in the second through hole; The clamping mechanism, the tray in / out mechanism, and the picking and clamping mechanism are all located in the upper space. The clamping mechanism can move between the storage mechanism, the transfer mechanism, the tray in / out mechanism, and the freezing tank to clamp the basket rack or the cryogenic box. The tray in / out mechanism is located below the clamping mechanism and can move back and forth between the storage mechanism and the transfer mechanism to carry the cryopreservation box; The picking and clamping mechanism is located above the freezing tank and can remove the target cryopreservation tube from one cryopreservation box and place the target cryopreservation tube into another cryopreservation box; The clamping mechanism includes a three-axis displacement stage and a basket module; The basket module has an extraction end and a connecting end. The connecting end is installed on the Z-axis of the three-axis displacement stage, which drives the extraction end to achieve vertical and horizontal movement. The extraction end has two extraction clips arranged opposite each other. The two extraction clips can move closer to each other or further apart, and the distance between the two extraction clips can be adjusted according to the different targets to be grasped. The pallet loading and unloading mechanism includes a slide rail and a support plate; The slide rail is installed on the middle platform, and the support plate is slidably installed on the slide rail; When the clamping mechanism clamps the basket frame out of the storage mechanism, the support plate slides into the basket frame. The clamping mechanism and the support plate in-and-out mechanism cooperate to transfer the cryopreservation boxes in the basket frame to the support plate, or to transfer the cryopreservation boxes on the support plate to the basket frame.

2. The cryogenic biobank device as described in claim 1, characterized in that, An insulation cover is also provided outside the upper space. The insulation cover is connected to the middle platform to form a sealed chamber, which is kept under a slight positive pressure.

3. The cryogenic biobank device as described in claim 1, characterized in that, The storage mechanism includes a tank body, an inner tank, and an insulated cover; The tank is fixedly installed in the lower space, and an annular flange is provided on the top edge of the tank. The top of the tank is sealed to the middle platform through the annular flange. The heat-insulating cover is disposed on the upper surface of the tank body, and the middle platform is provided with a third through hole, the position of which corresponds to the heat-insulating cover; The inner tank is rotatably installed inside the tank body via a drive mechanism; The basket frame consists of multiple baskets, which are arranged in a ring array with the central axis of the inner tank as the array center; The drive mechanism is capable of rotating the target basket frame to below the insulation cover.

4. The cryogenic biobank device as described in claim 3, characterized in that, The middle platform is equipped with a ventilation ring and a hot air blower; The ventilation ring is fitted onto the central axis of the inner tank, and the hot air blower is connected to the ventilation ring through a pipe.

5. The cryogenic biobank device as described in claim 3, characterized in that, The clamping mechanism also includes a lid lifting module; The lid-lifting module is fixed on the X-axis of the three-axis displacement stage and is used to open the heat-insulating lid to expose the basket frame.

6. The cryogenic biobank device as described in claim 5, characterized in that, The extraction end is wrapped with a cold insulation cover. The structural dimensions of the cold insulation cover are set according to the external dimensions of the basket frame. A gap is reserved between the bottom of the cold insulation cover and the tank body.

7. The cryogenic biobank device as described in claim 1, characterized in that, The transfer mechanism includes a lifting device, a transfer tank, and a position sensor; The lifting device is installed in the lower space; The transfer tank is mounted on the lifting device; The position sensor is mounted on the lifting device to detect whether the transfer tank has been raised into the upper space; A sealing ring is provided on the middle platform, and the sealing ring surrounds the first through hole. When the transfer tank moves to the upper space through the first through hole, it achieves a sealed connection with the middle platform by squeezing the sealing ring.

8. The cryogenic biobank device as described in claim 1, characterized in that, The frame platform has a built-in barcode scanner located between the transfer mechanism and the tray entry / exit mechanism, which is used to identify the cryopreservation box.

9. The cryogenic biobank device as described in claim 1, characterized in that, The picking and clamping mechanism includes a clamping module and a picking pin module; Both the clamping module and the tube-picking pin module are positioned above the freezing tank. The clamping module is used to position the first cryopreservation box and the second cryopreservation box in the freezing tank. The tube-picking pin module is used to pick up the target cryopreservation tube in the first cryopreservation box and place the target cryopreservation tube in the second cryopreservation box.

10. A method for storing and retrieving cryogenic biological samples, characterized in that, The cryogenic biobank equipment as described in any one of claims 1-9 is used to access and store cryopreservation boxes within the cryogenic biobank equipment, the access method comprising the steps of: The transfer mechanism is driven to rise, so that the first cryopreservation box inside the transfer mechanism is transferred from the lower space to the upper sealed chamber. The first cryopreservation box is transferred from the transfer mechanism to the tray in / out mechanism via a clamping mechanism; The clamping mechanism is used to clamp the basket rack inside the storage mechanism; The pallet in / out mechanism is driven to move, and the clamping mechanism is used to transfer the first cryopreservation box into the basket frame. The basket frame is placed into the storage mechanism by the clamping mechanism to complete the storage of the first cryopreservation box; The clamping mechanism is used to clamp the basket rack inside the storage mechanism; Drive the pallet in / out mechanism to move, and cooperate with the clamping mechanism to transfer the first cryopreservation box in the basket frame to the pallet in / out mechanism; The clamping mechanism is used to clamp the first cryopreservation box located on the tray in / out mechanism into the cryopreservation tank. The picking and clamping mechanism is used to pick up the target cryopreservation tube in the first cryopreservation box and transfer it into the second cryopreservation box. The clamping mechanism is used to clamp the second cryopreservation box containing the target cryopreservation tube onto the transfer mechanism. The transfer mechanism is driven to descend, transferring the second cryopreservation box and the target cryopreservation tube from the upper sealed chamber to the lower space, thereby completing the extraction of the second cryopreservation box and the target cryopreservation tube.

Images