A device for expanding bacillus

Abstract

The present application relates to the technical field of strain propagation, in particular to a bacillus propagation device, which comprises a container and a sealing assembly, the container is coaxially provided with a first fixing ring and a second fixing ring, a first feeding hole is formed in the top end of the container, the sealing assembly comprises a sealing plug and a movable ring, three second feeding holes are formed in the sealing plug, the sealing plug works in cooperation with the first feeding hole, the sealing plug is slidably connected with the movable ring, the sliding direction of the sealing plug is parallel to the axis of the movable ring, a first fixing hole is formed in the first fixing ring, which can fix the position of the movable ring, a second fixing hole is formed in the second fixing ring, which can fix the position of the sealing plug when the sealing plug blocks the first feeding hole, and the second fixing hole can fix the position of the sealing plug when the sealing plug does not block the first feeding hole. The present application is convenient to seal the container by using the sealing assembly, and is also convenient to check and maintain the sealing assembly in case of failure.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of strain propagation, in particular to a bacillus propagation device. BACKGROUND

[0002] When propagating strains, the strains in the container need to be fed, gas is sent and gas is discharged, and at the same time, the gas in the container is prevented from directly contacting the atmosphere. The strain propagation device in the prior art directly uses a traditional container for storage, lacks separate gas inlet, gas outlet and feeding channels, and thus the strain propagation efficiency is very low, and the operation is also relatively troublesome.

[0003] Chinese patent CN204752691U discloses a strain propagation device, which connects a container, a gas exchange device and a container feeding device, so as to ensure that the container interior can be operated separately through the gas inlet pipe, the gas outlet pipe and the feeding pipe while providing a better propagation environment, thereby effectively improving the strain propagation efficiency. However, in the use process, the sealing device is fixed by inserting a bottle plug and fixing it with a clamp. When the gas inlet pipe, the gas outlet pipe and the feeding pipe malfunction, the clamp needs to be disassembled and the sealing device needs to be repaired. After the repair is completed, the clamp is fixed again. The entire operation process is relatively complex and inconvenient. SUMMARY

[0004] In view of the above problems, a bacillus propagation device is provided, which fixes the sealing assembly for the first time through the first fixing ring, fixes the sealing assembly for the second time through the second fixing ring, and fixes the position of the sealing plug in the two states of plugging and unplugging through the sealing plug and the movable ring.

[0005] To solve the problems in the prior art, the technical scheme adopted by the present application is as follows:

[0006] A bacillus propagation device is provided, which includes a container and a sealing assembly. The first fixing ring and the second fixing ring are coaxially fixed on the container. The inner diameter of the first fixing ring is larger than the outer diameter of the second fixing ring. The top end of the container is provided with a first feeding hole, and the outer diameter of the first feeding hole is equal to the inner diameter of the second fixing ring. The first fixing ring and the second fixing ring are fixed on the outside of the top end of the container. The axis of the first fixing ring is coaxial with the axis of the first feeding hole. The sealing assembly includes a sealing plug and a movable ring. Three second feeding holes are formed in the sealing plug. The sealing plug works in cooperation with the first feeding hole. The sealing plug is slidably connected with the movable ring, and the sliding direction of the sealing plug is parallel to the axis of the movable ring. A first fixing hole is formed in the first fixing ring, which can fix the position of the movable ring. A second fixing hole is formed in the second fixing ring, which can fix the position of the sealing plug when the sealing plug blocks the first feeding hole. The second fixing ring can fix the position of the sealing plug when the sealing plug does not block the first feeding hole.

[0007] Preferably, the sealing assembly further comprises a first sliding block, the movable ring is fixedly provided with a first protrusion extending along the axial direction of the movable ring, the first sliding block is slidingly arranged at the end of the first protrusion, the sliding direction of the first sliding block is parallel to the diameter direction of the movable ring, and the first sliding block is axially and radially fixed to the movable ring in cooperation with the first fixed hole.

[0008] Preferably, the sealing assembly further comprises a first spring, the first spring is fixedly arranged between the first sliding block and the first protrusion, and the axis of the first spring is parallel to the sliding direction of the first sliding block.

[0009] Preferably, the sealing assembly further comprises a second sliding block and a second spring, the sealing plug is fixedly provided with a second protrusion extending along the axial direction of the movable ring, the second sliding block is slidingly arranged on the second protrusion, the sliding direction of the second sliding block is parallel to the diameter direction of the movable ring, the second sliding block works in cooperation with the second fixed hole, the second spring is fixedly arranged between the second sliding block and the second protrusion, and the axis of the second spring is parallel to the sliding direction of the second sliding block.

[0010] Preferably, the sealing assembly further comprises a first control ring and a sliding bar, the first control ring is rotatably connected to the sealing plug, the axis of the first control ring is coaxial with the axis of the movable ring, the inner wall of the first control ring is provided with an internal thread, the sliding bar is slidingly arranged on the sealing plug, the sliding direction of the sliding bar is parallel to the axis of the first control ring, the sliding bar is in threaded cooperation with the first control ring, and the second sliding block is provided with an inclined surface for cooperating with the sliding bar.

[0011] Preferably, the first control ring is coaxially provided with an annular groove, and a torsion spring is fixedly arranged in the annular groove in a coaxial manner.

[0012] Preferably, the sealing assembly further comprises a third spring, the sealing plug is fixedly provided with a guide rod, the axis of the guide rod is parallel to the axis of the movable ring, the movable ring is fixedly provided with a third protrusion extending along the diameter direction of the movable ring, the third protrusion is slidingly connected to the guide rod, the third spring is sleeved on the guide rod, and the third sliding block is slidingly arranged on the sealing plug.

[0013] Preferably, the container is slidingly provided with a second control ring, the second control ring is coaxially sleeved on the first fixed ring, the second control ring is fixedly provided with a fourth protrusion extending along the diameter direction, and the first sliding block is provided with an inclined surface for cooperating with the fourth protrusion.

[0014] Preferably, the second fixed ring is coaxially fixedly provided with a guide ring for cooperating with the first protrusion, the number of the first protrusions is several, the number of the second protrusions and the third protrusions is the same as that of the first protrusions, and all the first protrusions, the second protrusions and the third protrusions are uniformly arranged around the axis of the movable ring.

[0015] A kind of Bacillus amplification method for use, adopt the device for use of a kind of Bacillus amplification, it includes the following steps:

[0016] S1, insert the sealing plug into the first feed hole, fix the position of the movable ring through the first fixed hole, and fix the position of the sealing plug inserted into the first feed hole through the second fixed hole;

[0017] S2, input raw materials into the container through the second feed hole;

[0018] S3, when the second feed hole cannot input raw materials, contact the second fixed hole to fix the sealing plug, control the sealing plug to separate from the first feed hole;

[0019] S4, at this time, the sealing plug is away from the position of the first feed hole and is fixed, and both ends of the second feed hole are in communication with the outside;

[0020] S5, after checking the condition of the second feed hole and processing, the sealing plug is fixed, and the sealing plug is automatically inserted into the first feed hole and fixed.

[0021] The beneficial effects of the present application compared with the prior art are:

[0022] The present application realizes the first fixing function of the sealing assembly by the first fixed ring, realizes the second fixing function of the sealing assembly by the second fixed ring, realizes the function that the position of the sealing plug in the two states of plugging and unplugging can be fixed by the sealing plug and the movable ring, so as to facilitate the sealing of the container by using the sealing assembly, and also facilitate the inspection and maintenance of the sealing assembly with fault. BRIEF DESCRIPTION OF DRAWINGS

[0023] Figure 1 It is a three-dimensional schematic diagram of a Bacillus amplification device Figure 1 ;

[0024] Figure 2 It is Figure 1 A partial enlarged schematic view of the A;

[0025] Figure 3 It is a three-dimensional schematic diagram of a Bacillus amplification device Figure 2 ;

[0026] Figure 4 It is Figure 3 A partial enlarged schematic view of the B;

[0027] Figure 5 It is a three-dimensional schematic diagram of a container in a Bacillus amplification device;

[0028] Figure 6 It is a three-dimensional exploded schematic diagram of a container in a Bacillus amplification device.

[0029] Figure 7 is Figure 6 a partial enlarged view of C in FIG. 1;

[0030] Figure 8 is a perspective view of a sealing assembly in a device for expanding bacillus;

[0031] Figure 9 is a perspective exploded view of a sealing assembly in a device for expanding bacillus;

[0032] Figure 10 is Figure 9 a partial enlarged view of D in FIG. 1.

[0033] Reference numerals in the drawings are:

[0034] 1 - container;

[0035] 11 - first fixing ring; 111 - first fixing hole;

[0036] 12 - second fixing ring; 121 - second fixing hole;

[0037] 13 - first feeding hole;

[0038] 14 - second control ring; 141 - fourth protrusion;

[0039] 15 - guide ring;

[0040] 2 - sealing assembly;

[0041] 21 - sealing plug; 211 - second feeding hole; 212 - second protrusion; 213 - guide rod;

[0042] 22 - movable ring; 221 - first protrusion; 222 - third protrusion;

[0043] 23 - first sliding block;

[0044] 24 - first spring;

[0045] 25 - second sliding block;

[0046] 26 - second spring;

[0047] 27 - first control ring; 271 - annular groove; 272 - torsion spring;

[0048] 28 - sliding bar;

[0049] 29 - third spring. DETAILED DESCRIPTION

[0050] In order to further understand the features, technical means and specific purposes and functions of the present application, the present application will be described in further detail below in conjunction with the drawings and specific embodiments.

[0051] Referring to Figures 1 to 10 As shown in the drawings, a device for expanding Bacillus includes a container 1 and a sealing assembly 2. The container 1 is coaxially provided with a first fixing ring 11 and a second fixing ring 12. The inner diameter of the first fixing ring 11 is greater than the outer diameter of the second fixing ring 12. The top end of the container 1 is provided with a first feeding hole 13. The outer diameter of the first feeding hole 13 is equal to the inner diameter of the second fixing ring 12. The first fixing ring 11 and the second fixing ring 12 are fixedly arranged outside the top end of the container 1. The axis of the first fixing ring 11 is coaxial with the axis of the first feeding hole 13. The sealing assembly 2 includes a sealing plug 21 and a movable ring 22. The sealing plug 21 is provided with three second feeding holes 211. The sealing plug 21 works in cooperation with the first feeding hole 13. The sealing plug 21 is slidably connected with the movable ring 22. The sliding direction of the sealing plug 21 is parallel to the axis of the movable ring 22. The first fixing ring 11 is provided with a first fixing hole 111 capable of fixing the position of the movable ring 22. The second fixing ring 12 is provided with a second fixing hole 121 capable of fixing the position of the sealing plug 21 when the sealing plug 21 blocks the first feeding hole 13. The second fixing ring 12 can fix the position of the sealing plug 21 when the sealing plug 21 does not block the first feeding hole 13.

[0052] The sealing plug 21 is aligned with the first feeding hole 13 and inserted. With the continuous insertion of the sealing plug 21, the first fixing hole 111 fixes the position of the movable ring 22, and at the same time, the second fixing hole 121 fixes the position of the sealing plug 21. At this time, the sealing plug 21 blocks the first feeding hole 13. Then, the raw materials for cultivation are continuously delivered into the container 1 through the second feeding hole 211. When the second feeding hole 211 cannot output the raw materials, the fixing of the sealing plug 21 by the second fixing hole 121 is released at this time. The sealing plug 21 is controlled to move away from the container 1. When the sealing plug 21 is separated from the first feeding hole 13, the second fixing ring 12 fixes the position of the sealing plug 21 at this time. The two ends of the second feeding hole 211 are both in communication with the outside. After checking the second feeding hole 211 and processing, the fixing of the position of the sealing plug 21 by the second fixing ring 12 at this time is released. The sealing plug 21 is inserted into the first feeding hole 13 again and fixed in position by the second fixing hole 121. Compared with the prior art, the first fixing ring 11 of the present application fixes the sealing assembly 2 for the first time. The second fixing ring 12 fixes the sealing assembly 2 for the second time. The sealing plug 21 and the movable ring 22 can fix the position of the sealing plug 21 in the two states of blocking and not blocking. Therefore, it is convenient to seal the container 1 by using the sealing assembly 2. At the same time, it is also convenient to check and maintain the sealing assembly 2 that fails.

[0053] Referring to Figure 3 , Figure 5 ,Figure 6 、 Figure 7 and Figure 8 As shown in

[0054] The movable ring 22 moves following the movement of the sealing plug 21, the first protrusion 221 is inserted between the first fixed ring 11 and the second fixed ring 12, when the end of the first protrusion 221 contacts the container 1, at this time, the first sliding block 23 is controlled to move away from the sealing plug 21 until it is inserted into the first fixed hole 111, compared with the prior art, the first sliding block 23 and the first fixed hole 111 of the present application cooperate to complete the first fixation of the sealing assembly, thereby avoiding the sealing plug 21 from easily separating from the container 1 after being released from fixation.

[0055] As shown in Figure 3 、 Figure 8 and Figure 9 The sealing assembly 2 further comprises a first spring 24, which is fixedly arranged between the first sliding block 23 and the first protrusion 221, and the axis of the first spring 24 is parallel to the sliding direction of the first sliding block 23.

[0056] When the first protrusion 221 does not enter between the first fixed ring 11 and the second fixed ring 12, the first protrusion 221 is controlled to move towards the sealing plug 21, at this time, the first spring 24 is compressed, and then the first protrusion 221 is controlled to drive the first sliding block 23 to enter between the first fixed ring 11 and the second fixed ring 12, when the first protrusion 221 contacts the container 1, the first spring 24 is released from the compressed state, and the first spring 24 pushes the first protrusion 221 to be inserted into the first fixed hole 111, compared with the prior art, the first spring 24 of the present application enables the first sliding block 23 between the first fixed ring 11 and the second fixed ring 12 to automatically move, thereby reducing the complexity of operation.

[0057] As shown in Figure 3 、 Figure 8 and Figure 9As shown in the figure, the sealing assembly 2 further comprises a second sliding block 25 and a second spring 26, the sealing plug 21 is fixedly provided with a second protrusion 212 extending along the axial direction of the movable ring 22, the second sliding block 25 is slidingly arranged on the second protrusion 212, the sliding direction of the second sliding block 25 is parallel to the diameter direction of the movable ring 22, the second sliding block 25 cooperates with the second fixed hole 121, and the second spring 26 is fixedly arranged between the second sliding block 25 and the second protrusion 212, the axis of the second spring 26 is parallel to the sliding direction of the second sliding block 25.

[0058] Before the sealing plug 21 is inserted into the second fixed ring 12, the second sliding block 25 is controlled to move close to the center of the sealing plug 21, the second spring 26 is compressed, then the sealing plug 21 is inserted into the second fixed ring 12, when the sealing plug 21 moves a certain distance, the second spring 26 is released from the compressed state, the second spring 26 pushes the second sliding block 25 to be inserted into the second fixed hole 121 to fix the sealing plug 21, at this time, the position of the movable ring 22 is also fixed, then the fixation of the second sliding block 25 and the second fixed hole 121 is released, the sealing plug 21 is controlled to move away from the container 1, when most of the area of the second protrusion 212 is separated from the second fixed ring 12, the second sliding block 25 protrudes from the outer side wall of the sealing plug 21, at this time, the position of the sealing plug 21 is fixed, compared with the prior art, the cooperation of the second sliding block 25, the second fixed ring 12 and the second fixed hole 121 makes the sealing plug 21 be fixed in position in two states, thereby facilitating the inspection and maintenance of the second feeding hole 211.

[0059] As shown in the figure, Figure 3 , Figure 8 and Figure 9 As shown in the figure, the sealing assembly 2 further comprises a first control ring 27 and a sliding bar 28, the first control ring 27 is rotatably connected with the sealing plug 21, the axis of the first control ring 27 is coaxial with the axis of the movable ring 22, the inner wall of the first control ring 27 is provided with an internal thread, the sliding bar 28 is slidingly arranged on the sealing plug 21, the sliding direction of the sliding bar 28 is parallel to the axis of the first control ring 27, the sliding bar 28 cooperates with the first control ring 27 in a threaded manner, and the second sliding block 25 is provided with an inclined surface cooperating with the sliding bar 28.

[0060] Rotate the first control ring 27, the first control ring 27 is screwed to make the sliding bar 28 move along the axis direction of the movable ring 22, when the sliding bar 28 moves, the second sliding block 25 will be moved along the diameter direction of the movable ring 22 through the inclined surface on the second sliding block 25, the second spring 26 is compressed, when the first control ring 27 is rotated in the opposite direction, the sliding bar 28 is separated from the second sliding block 25, the second sliding block 25 moves under the elastic force of the second spring 26, compared with the prior art, the first control ring 27 and the sliding bar 28 of the present application cooperate to rotate the first control ring 27 to control the movement of the second sliding block 25, so that the movement of the second sliding block 25 is convenient to control.

[0061] Referring to Figures 8 to 10 As shown in the figure: the first control ring 27 is coaxially provided with an annular groove 271, and a torsion spring 272 is fixed coaxially in the annular groove 271.

[0062] Rotate the first control ring 27, the torsion spring 272 will be compressed, and the second sliding block 25 will move towards the center of the sealing plug 21. Release the first control ring 27, the torsion spring 272 will be in a state of decompression and push the first control ring 27 to move to the initial position, and the second sliding block 25 will move away from the center of the sealing plug 21. Compared with the prior art, the torsion spring 272 of the present application provides a force to limit the position of the first control ring 27, thereby avoiding the first control ring 27 from sliding randomly and thus releasing the sealing plug 21.

[0063] Referring to Figure 3 , Figure 8 and Figure 9 As shown in the figure: the sealing assembly 2 further comprises a third spring 29, a guide rod 213 is fixedly arranged on the sealing plug 21, the axis of the guide rod 213 is parallel to the axis of the movable ring 22, a third protrusion 222 extending along the diameter direction of the movable ring 22 is fixedly arranged on the movable ring 22, the third protrusion 222 is slidably connected with the guide rod 213, the third spring 29 is sleeved on the guide rod 213, and a third sliding block is slidably arranged on the sealing plug 21.

[0064] After the first control ring 27 is accidentally rotated, due to the presence of the third spring 29, the sealing plug 21 is at the lowest point of the movable range compared with the movable ring 22, and the position of the movable ring 22 is fixed. Compared with the prior art, the third spring 29 of the present application limits the relative position of the sealing plug 21 and the movable ring 22, thereby ensuring that the sealing plug 21 which is released by default blocks the first feeding hole 13.

[0065] Referring to Figure 3 , Figure 5 , Figure 6 and Figure 7As shown: the second control ring 14 is coaxially sleeved on the first fixed ring 11, and the fourth protrusion 141 extending in the diameter direction is fixedly arranged on the second control ring 14, and the inclined surface matched with the fourth protrusion 141 is arranged on the first sliding block 23.

[0066] When the second control ring 14 is controlled to move away from the container 1, the fourth protrusion 141 first contacts the inclined surface of the first sliding block 23, so that the first sliding block 23 is separated from the first fixed hole 111, and at this time, the fixing of the movable ring 22 is released, compared with the prior art, the second control ring 14 of the present application can conveniently release the fixing of the movable ring 22, thereby facilitating the disassembly of the whole sealing assembly 2.

[0067] Referring to Figure 3 、 Figure 5 、 Figure 6 and Figure 7 As shown: the guide ring 15 matched with the first protrusion 221 is coaxially fixed on the second fixed ring 12, the number of the first protrusion 221 is several, the number of the second protrusion 212 and the third protrusion 222 is the same as that of the first protrusion 221, and all the first protrusion 221, the second protrusion 212 and the third protrusion 222 are uniformly arranged around the axis of the movable ring 22.

[0068] When the first protrusion 221 contacts the guide ring 15, it will be offset, and when the first protrusion 221 moves to the end of the guide ring 15 close to the second fixed ring 12, the sealing plug 21 is coaxial with the second fixed ring 12, compared with the prior art, the guide ring 15 of the present application limits the axis of the sealing plug 21, thereby reducing the time spent on aligning the axis of the sealing plug 21 with the first feeding hole 13.

[0069] Referring to Figures 1 to 10 As shown: a method for expanding Bacillus, using the device for expanding Bacillus, comprising the following steps:

[0070] S1, the sealing plug 21 is inserted into the first feeding hole 13, the position of the movable ring 22 is fixed through the first fixed hole 111, and the position of the sealing plug 21 inserted into the first feeding hole 13 is fixed through the second fixed hole 121;

[0071] S2, the raw materials are input into the container 1 through the second feeding hole 211;

[0072] S3, when the second feeding hole 211 cannot input raw materials, the fixing of the sealing plug 21 by the second fixed hole 121 is contacted, and the sealing plug 21 is controlled to be separated from the first feeding hole 13;

[0073] S4, at this time, the sealing plug 21 is in a position away from the first feeding hole 13 and is fixed, and the two ends of the second feeding hole 211 are in communication with the outside.

[0074] S5, after checking the second feed hole 211 and processing, the fixing of the plug 21 is released, the plug 21 is automatically inserted into the first feed hole 13 and fixed.

[0075] Compared with the prior art, the first fixed ring 11 of the application fixes the sealing assembly 2 for the first time, the second fixed ring 12 fixes the sealing assembly 2 for the second time, and the plug 21 and the movable ring 22 make the plug 21 be fixed in the position of the two states of blocking and unblocking, so as to facilitate the sealing of the container 1 by using the sealing assembly 2, and also facilitate the inspection and maintenance of the sealing assembly 2 with faults.

[0076] The above embodiments only express one or several embodiments of the application, and the description is more specific and detailed, but it cannot be understood as the limitation of the patent scope of the application. It should be pointed out that for ordinary skilled in the art, without departing from the concept of the application, a number of modifications and improvements can be made, which belong to the protection scope of the application. Therefore, the protection scope of the patent of the application should be subject to the appended claims.

Claims

1. A device for expanding bacillus, comprising a container (1) and a sealing assembly (2), characterized in that, the container (1) is coaxially provided with a first fixed ring (11) and a second fixed ring (12), the inner diameter of the first fixed ring (11) is larger than the outer diameter of the second fixed ring (12), the top end of the container (1) is provided with a first feeding hole (13), the outer diameter of the first feeding hole (13) is equal to the inner diameter of the second fixed ring (12), the first fixed ring (11) and the second fixed ring (12) are fixedly arranged outside the top end of the container (1), and the axis of the first fixed ring (11) is coaxial with the axis of the first feeding hole (13); the sealing assembly (2) comprises a sealing plug (21) and a movable ring (22), the sealing plug (21) is provided with three second feeding holes (211), the sealing plug (21) cooperates with the first feeding hole (13), the sealing plug (21) is slidably connected with the movable ring (22), the sliding direction of the sealing plug (21) is parallel to the axis of the movable ring (22), the first fixed ring (11) is provided with a first fixed hole (111) capable of fixing the position of the movable ring (22), and the second fixed ring (12) is provided with a second fixed hole (121) capable of fixing the position of the sealing plug (21) when the sealing plug (21) blocks the first feeding hole (13); the second fixed ring (12) can fix the position of the sealing plug (21) when the sealing plug (21) does not block the first feeding hole (13); the sealing assembly (2) further comprises a first sliding block (23); the movable ring (22) is fixedly provided with a first protrusion (221) extending along the axis direction of the movable ring (22), the first sliding block (23) is slidably arranged at the end of the first protrusion (221), the sliding direction of the first sliding block (23) is parallel to the diameter direction of the movable ring (22), and the first sliding block (23) cooperates with the first fixed hole (111) to axially and radially fix the movable ring (22); the sealing assembly (2) further comprises a first spring (24); the first spring (24) is fixedly arranged between the first sliding block (23) and the first protrusion (221), and the axis of the first spring (24) is parallel to the sliding direction of the first sliding block (23); the sealing assembly (2) further comprises a second sliding block (25) and a second spring (26); the sealing plug (21) is fixedly provided with a second protrusion (212) extending along the axis direction of the movable ring (22), the second sliding block (25) is slidably arranged on the second protrusion (212), the sliding direction of the second sliding block (25) is parallel to the diameter direction of the movable ring (22), the second sliding block (25) cooperates with the second fixed hole (121), the second spring (26) is fixedly arranged between the second sliding block (25) and the second protrusion (212), and the axis of the second spring (26) is parallel to the sliding direction of the second sliding block (25).

2. The device for propagating bacillus according to claim 1, characterized in that, the sealing assembly (2) further comprises a first control ring (27) and a sliding bar (28); The first control ring (27) is rotatably connected with the sealing plug (21), the axis of the first control ring (27) is coaxial with the axis of the movable ring (22), the inner wall of the first control ring (27) is provided with an internal thread, the sliding bar (28) is slidingly arranged on the sealing plug (21), the sliding direction of the sliding bar (28) is parallel to the axis of the first control ring (27), the sliding bar (28) is threadedly connected with the first control ring (27), and the second sliding block (25) is provided with a slope matched with the working of the sliding bar (28).

3. The device for propagating bacillus according to claim 2, characterized in that, The first control ring (27) is coaxially provided with an annular groove (271), and the torsional spring (272) is fixedly arranged in the annular groove (271).

4. The device for propagating bacillus according to claim 3, characterized in that, The sealing assembly (2) further comprises a third spring (29); The sealing plug (21) is fixedly provided with a guide rod (213), the axis of the guide rod (213) is parallel to the axis of the movable ring (22), the movable ring (22) is fixedly provided with a third protrusion (222) extending along the diameter direction of the movable ring (22), the third protrusion (222) is slidingly connected with the guide rod (213), the third spring (29) is sleeved on the guide rod (213), and the third sliding block is slidingly arranged on the sealing plug (21).

5. The device for propagating bacillus according to claim 1, wherein The second control ring (14) is slidingly arranged on the container (1), the second control ring (14) is coaxially sleeved on the first fixed ring (11), the fourth protrusion (141) extending along the diameter direction is fixedly arranged on the second control ring (14), and the first sliding block (23) is provided with a slope matched with the working of the fourth protrusion (141).

6. The device for propagating bacillus according to claim 4, wherein The second fixed ring (12) is coaxially fixedly provided with a guide ring (15) matched with the working of the first protrusion (221), the number of the first protrusion (221) is several, the number of the second protrusion (212) and the third protrusion (222) is the same as that of the first protrusion (221), and all the first protrusion (221), the second protrusion (212) and the third protrusion (222) are uniformly arranged around the axis of the movable ring (22).

7. A method for propagating Bacillus using the device for propagating Bacillus according to any one of claims 1 to 6, wherein the device is used for propagating Bacillus. The method comprises the following steps: S1, the sealing plug (21) is inserted into the first feeding hole (13), the position of the movable ring (22) is fixed through the first fixed hole (111), and the position of the sealing plug (21) inserted into the first feeding hole (13) is fixed through the second fixed hole (121); S2, the raw materials are input into the container (1) through the second feeding hole (211); S3, when the second feeding hole (211) cannot input raw materials, the fixing of the sealing plug (21) to the second fixed hole (121) is contacted, and the sealing plug (21) is controlled to be separated from the first feeding hole (13); S4, at this time, the sealing plug (21) is away from the first feeding hole (13) and is fixed, and the two ends of the second feeding hole (211) are in communication with the outside; S5, after checking the condition of the second feeding hole (211) and processing, the fixing of the sealing plug (21) is released, the sealing plug (21) is automatically inserted into the first feeding hole (13) and is fixed.

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