A method for integrated prevention and control of vegetable diseases and insect pests in solar greenhouses

Abstract

The present invention relates to the field of agricultural planting technology, and in particular to a method for integrated pest control of vegetable diseases and insect pests in a solar greenhouse. The present invention provides a method for integrated pest control of vegetable diseases and insect pests in a solar greenhouse, comprising the following steps: (1) before planting the vegetables, applying base fertilizer, rotary tilling, covering the vegetables with film, sealing the greenhouse, and applying probiotics; (2) after planting the vegetables, when diseases occur, spraying biological microbial agents; when insect pests occur, spraying insecticides. The control method provided by the present invention has a good control effect on a variety of diseases and insect pests of various vegetables, and the effective ingredients of the biological microbial agents and insecticides of the present invention have a synergistic effect, have a good inhibitory effect on diseases and insect pests, and have the effect of repelling and inhibiting the reproduction of conventional crop pests, etc., and have excellent use effect.

Description

Technical Field

[0001] The present invention relates to the technical field of agricultural pest control, and in particular to a method for integrated pest control of vegetables in a solar greenhouse. Background Art

[0002] Vegetables are one of the essential foods in people's daily diet. Vegetables can provide the human body with a variety of nutrients such as vitamins and minerals.

[0003] According to statistics from the Food and Agriculture Organization of the United Nations in 1990, 90% of the human body's essential vitamin C and 60% of its vitamin A come from vegetables. Furthermore, vegetables contain a wide variety of phytochemicals, which are widely recognized as beneficial for health. Traditional vegetable cultivation is done outdoors, which is cost-effective but prone to pests and diseases. For example, lettuce is particularly susceptible to diseases such as downy mildew, gray mold, brown spot, black spot, wilt, sclerotinia, soft rot, leaf rot, leaf edge necrosis, nematode disease, and viral diseases.

[0004] Nowadays, people are starting to grow vegetables in greenhouses. The greenhouse environment is relatively independent, and the occurrence of pests and diseases is reduced compared to open-air cultivation. However, in actual cultivation, it has been found that sporadic outbreaks of diseases such as gray mold, downy mildew, brown spot, black spot, sclerotinia, and viral diseases of lettuce have occurred in the hydroponic and substrate cultivation areas of greenhouse vegetables. At the same time, pests such as leaf miners, winter armyworms, and aphids have also been found to cause damage. Greenhouse vegetables have a short growing period, and some are intended for raw consumption. In order to meet industry standards, greenhouse vegetable pest and disease control cannot completely adopt the control plan for open-air vegetables, otherwise it will lead to excessive pesticide residues.

[0005] Therefore, how to provide a green pest control method for greenhouse vegetable pests and diseases to solve the technical problems of greenhouse vegetable pest control in the existing technology is an issue that needs to be urgently solved by those skilled in the art. Summary of the Invention

[0006] The purpose of the present invention is to provide a method for integrated pest control of greenhouse vegetables, so as to solve the technical problem of poor pest control effect of greenhouse vegetables in the prior art.

[0007] In order to achieve the above-mentioned object of the invention, the present invention provides the following technical solutions:

[0008] The present invention provides a method for integrated pest control of vegetables in a solar greenhouse, comprising the following steps:

[0009] (1) Before planting vegetables, apply base fertilizer, till the soil, cover the soil with film, and apply probiotics;

[0010] (2) After vegetable planting

[0011] When the disease occurs, spraying biological agents: the biological agents include: Bacillus licheniformis, Bacillus amyloliquefaciens, Trichoderma viride, Beauveria bassiana and Rhodotorula;

[0012] When insect pests occur, insect repellent is sprayed; the insect repellent includes: chlorfluazuron, cypermethrin, oleander extract, cnidium monnieri extract, pulsatilla scabra extract, chinaberry bark extract, sophora flavescens extract and astragalus extract.

[0013] Preferably, the base fertilizer in step (1) comprises: waste fungus residue, poultry and livestock manure, straw, soybean meal and wood ash, with a mass ratio of 12-16:45-65:8-12:4-8:5-9.

[0014] Preferably, the depth of the rotary tillage in step (1) is 10 to 20 cm.

[0015] Preferably, the time of the sealed greenhouse in step (1) is 12 to 18 days; and the soil moisture content is maintained at 65 to 75% during the sealed greenhouse.

[0016] Preferably, the probiotics in step (1) are EM compound bacteria; the application amount of the EM compound bacteria is 4 to 8 kg / mu.

[0017] Preferably, in step (2), the bacterial content of the Bacillus licheniformis is 400-800 million / g; the bacterial content of the Bacillus amyloliquefaciens is 300-700 million / g; the bacterial content of the Trichoderma viride is 400-600 million / g; the bacterial content of the Beauveria bassiana is 200-400 million / g; and the bacterial content of the red yeast is 200-400 million / g.

[0018] Preferably, the mass ratio of the Bacillus licheniformis, Bacillus amyloliquefaciens, Trichoderma viride, Beauveria bassiana and Rhodotorula is 8-12:6-10:8-12:4-6:3-5.

[0019] Preferably, the mass ratio of chlorfluazuron, cypermethrin, oleander extract, cnidium monnieri extract, pulsatilla scabra extract, chinaberry bark extract, sophora flavescens extract and astragalus extract in step (2) is 2-6:4-8:5-9:12-16:7-13:8-12:9-13:13-17.

[0020] Preferably, in step (1), probiotics are applied within 3 to 5 days after the end of the shed.

[0021] Preferably, the vegetable is lettuce, cabbage or water spinach.

[0022] Compared with the prior art, the present invention has the following beneficial effects:

[0023] 1. Although the sealing treatment can effectively kill pathogens or pests, it will also significantly affect the content of beneficial bacteria in the soil. After the sealing treatment is completed, there are very few beneficial bacteria left in the soil. The present invention applies the EM composite bacterial agent after sealing the greenhouse, which can effectively supplement the content of beneficial bacteria in the soil. The base fertilizer applied in the early stage is also decomposed after the sealing, and the nutrients contained therein are conducive to the colonization and reproduction of beneficial bacteria. The large-scale reproduction of beneficial bacteria has an antagonistic effect on the reproduction of harmful bacteria in the soil, and also has an excellent growth-promoting effect on the growth of vegetables.

[0024] 2. The present invention utilizes chemical agents in combination with traditional Chinese medicine preparations to prepare insect repellents. In this invention, the chemical agents and traditional Chinese medicine preparations not only achieve a synergistic effect, but the addition of the traditional Chinese medicine preparations also significantly reduces the use of chemical agents. Because traditional Chinese medicine preparations are green and residue-free, the insect repellent of the present invention leaves very little pesticide residue in vegetables, and after the vegetables are harvested, there is no excessive pesticide residue.

[0025] 3. The biological agent added in the present invention plays the role of antagonist of harmful bacteria. After the application of the biological agent, the growth and reproduction of harmful bacteria can be inhibited in a short time. After spraying again or multiple times, the occurrence or spread of vegetable diseases can be effectively inhibited.

[0026] 4. The control method provided by the present invention has a good control effect on a variety of diseases and pests of various vegetables, and there is a synergistic effect between the biological bacterial agent and the active ingredients of the insecticide of the present invention, which has a good inhibitory effect on diseases and pests. At the same time, it has the effect of repelling and inhibiting the reproduction of conventional crop pests, and has excellent use effect. DETAILED DESCRIPTION

[0027] The present invention provides a method for integrated pest control of vegetables in a solar greenhouse, comprising the following steps:

[0028] (1) Before planting vegetables, apply base fertilizer, till the soil, cover the soil with film, and apply probiotics;

[0029] (2) After vegetable planting

[0030] When the disease occurs, spraying biological agents: the biological agents include: Bacillus licheniformis, Bacillus amyloliquefaciens, Trichoderma viride, Beauveria bassiana and Rhodotorula;

[0031] When insect pests occur, insect repellent is sprayed; the insect repellent includes: chlorfluazuron, cypermethrin, oleander extract, cnidium monnieri extract, pulsatilla scabra extract, chinaberry bark extract, sophora flavescens extract and astragalus extract.

[0032] In the present invention, the base fertilizer in step (1) comprises: waste fungus residue, poultry and livestock manure, straw, soybean meal and wood ash, with a mass ratio of 12-16:45-65:8-12:4-8:5-9; preferably 13-15:50-60:9-11:5-7:6-8; further preferably 14:55:10:6:7.

[0033] In the present invention, the depth of the rotary tillage in step (1) is 10 to 20 cm; preferably 12 to 18 cm; more preferably 14 to 16 cm; and more preferably 15 cm.

[0034] In the present invention, the time of the stuffy chamber in step (1) is 12 to 18 days; preferably 13 to 17 days; more preferably 14 to 16 days; and more preferably 15 days.

[0035] In the present invention, during the sealing of the greenhouse in step (1), the soil moisture content is maintained at 65-75%; preferably 67-73%; more preferably 69-71%; and even more preferably 70%.

[0036] In the present invention, the probiotics in step (1) are EM compound bacteria; the application amount of the EM compound bacteria is 4 to 8 kg / mu; preferably 5 to 7 kg / mu; more preferably 6 kg / mu.

[0037] In the present invention, the bacterial content of the Bacillus licheniformis in step (2) is 400-800 million / g; the bacterial content of the Bacillus amyloliquefaciens is 300-700 million / g; the bacterial content of the Trichoderma viride is 400-600 million / g; the bacterial content of the Beauveria bassiana is 200-400 million / g; the bacterial content of the red yeast is 200-400 million / g;

[0038] Preferably, the bacterial content of the Bacillus licheniformis is 500-700 million / g; the bacterial content of the Bacillus amyloliquefaciens is 400-600 million / g; the bacterial content of the Trichoderma viride is 500 million / g; the bacterial content of the Beauveria bassiana is 300 million / g; and the bacterial content of the red yeast is 300 million / g.

[0039] It is further preferred that the bacterial content of the Bacillus licheniformis is 600 million / g; the bacterial content of the Bacillus amyloliquefaciens is 500 million / g; the bacterial content of the Trichoderma viride is 500 million / g; the bacterial content of the Beauveria bassiana is 300 million / g; and the bacterial content of the red yeast is 300 million / g.

[0040] In the present invention, the mass ratio of Bacillus licheniformis, Bacillus amyloliquefaciens, Trichoderma viride, Beauveria bassiana and red yeast is 8-12:6-10:8-12:4-6:3-5; preferably 9-11:7-9:9-11:5:4; more preferably 10:8:10:5:4.

[0041] In the present invention, the mass ratio of chlorfluazuron, cypermethrin, oleander extract, cnidium monnieri extract, pulsatilla scabra extract, chinaberry bark extract, sophora flavescens extract and astragalus extract in step (2) is 2-6:4-8:5-9:12-16:7-13:8-12:9-13:13-17; preferably 3-5:5-7:6-8:13-15:8-12:9-11:10-12:14-16; further preferably 4:6:7:14:9-11:10:14:15; more preferably 4:6:7:14:10:10:14:15.

[0042] In the present invention, in step (1), probiotics are applied within 3 to 5 days after the end of the shed, preferably within 4 days.

[0043] In the present invention, the vegetable is lettuce, cabbage or water spinach; preferably lettuce.

[0044] In the present invention, the EM composite bacterial agent is Shanxi Linfen Diliwang EM bacteria (Weifang Nongyi Biotechnology Co., Ltd.).

[0045] The technical solutions provided by the present invention are described in detail below with reference to the embodiments, but they should not be construed as limiting the scope of protection of the present invention.

[0046] Example 1

[0047] A method for integrated pest control of vegetables in a solar greenhouse, comprising the following steps:

[0048] (1) Mixing waste mushroom residue, livestock manure, corn straw, soybean meal and wood ash in a mass ratio of 12:45:8:4:5 to obtain base fertilizer;

[0049] The livestock manure includes sheep manure, pig manure and cow manure, with a mass ratio of 50:15:15;

[0050] (2) Bacillus licheniformis, Bacillus amyloliquefaciens, Trichoderma viride, Beauveria bassiana, and Rhodotorula were mixed in a mass ratio of 8:6:8:4:3 to obtain a biological agent;

[0051] The bacterial content of the Bacillus licheniformis is 400 million / g; the bacterial content of the Bacillus amyloliquefaciens is 500 million / g; the bacterial content of the Trichoderma viride is 400 million / g; the bacterial content of the Beauveria bassiana is 200 million / g; the bacterial content of the red yeast is 200 million / g;

[0052] (3) oleander, cnidium monnieri, pulsatilla striata, chinaberry bark, sophora flavescens and astragalus were mixed with water at a mass ratio of 1:3 and decocted for 2 h, and the residue was removed to obtain oleander extract, cnidium monnieri extract, pulsatilla striata extract, chinaberry bark extract, sophora flavescens extract and astragalus extract, and the different Chinese herbal extracts were mixed to obtain a Chinese herbal extract;

[0053] (4) mixing the Chinese herbal extract with 400 times of 5% emulsifiable concentrate of fluazifop and 600 times of 10% emulsifiable concentrate of cypermethrin to obtain an insect repellent;

[0054] The mass ratio of 400 times diluted 5% emulsifiable concentrate of fluazifop, 600 times diluted 10% emulsifiable concentrate of cypermethrin, oleander extract, Cnidium monnieri extract, Pulsatilla scabra extract, Melia azedarach bark extract, Sophora flavescens extract and Astragalus membranaceus extract is 2:4:5:12:7:8:9:13;

[0055] (5) Before planting water spinach, apply 300 kg / mu of base fertilizer and then perform rotary tillage to a depth of 10 cm;

[0056] (6) After rotary tillage, cover the planting land with film, keep the greenhouse closed, and then seal the greenhouse for 12 days, maintaining the soil moisture content at 65%;

[0057] (7) 4 days after the greenhouse is closed, apply 4 kg / mu of probiotic EM compound agent;

[0058] (8) After planting water spinach

[0059] When water spinach disease first occurs, dilute the biological agent with water 8 times and spray until water droplets fall off; spray once every 5 days for 3 consecutive times;

[0060] When water spinach pests first occur, dilute the insect repellent 8 times with water and spray until water droplets fall off; spray once every 7 days for 2 consecutive times.

[0061] Example 2

[0062] A method for integrated pest control of vegetables in a solar greenhouse, comprising the following steps:

[0063] (1) Mixing waste mushroom residue, livestock manure, corn straw, soybean meal and wood ash in a mass ratio of 16:65:12:8:9 to obtain base fertilizer;

[0064] The livestock manure includes sheep manure, pig manure and cow manure, with a mass ratio of 70:25:25.

[0065] (2) Bacillus licheniformis, Bacillus amyloliquefaciens, Trichoderma viride, Beauveria bassiana, and Rhodotorula were mixed in a mass ratio of 12:10:12:6:5 to obtain a biological agent;

[0066] The bacterial content of the Bacillus licheniformis is 800 million / g; the bacterial content of the Bacillus amyloliquefaciens is 700 million / g; the bacterial content of the Trichoderma viride is 600 million / g; the bacterial content of the Beauveria bassiana is 400 million / g; and the bacterial content of the red yeast is 400 million / g.

[0067] (3) oleander, cnidium monnieri, pulsatilla striata, chinaberry bark, sophora flavescens and astragalus were mixed with water at a mass ratio of 1:5 and boiled for 4 h, and the residue was removed to obtain oleander extract, cnidium monnieri extract, pulsatilla striata extract, chinaberry bark extract, sophora flavescens extract and astragalus extract, and the different Chinese herbal extracts were mixed to obtain a Chinese herbal extract;

[0068] (4) mixing the Chinese herbal extract with 400 times of 5% emulsifiable concentrate of fluazifop and 600 times of 10% emulsifiable concentrate of cypermethrin to obtain an insect repellent;

[0069] The mass ratio of 400 times diluted 5% emulsifiable concentrate of fluazifop, 600 times diluted 10% emulsifiable concentrate of cypermethrin, oleander extract, cnidium monnieri extract, pulsatilla scabra extract, chinaberry bark extract, sophora flavescens extract and astragalus extract is 6:8:9:16:13:12:13:17;

[0070] (5) Before planting cabbage, apply 400 kg / mu of base fertilizer and then perform rotary tillage to a depth of 20 cm;

[0071] (6) After rotary tillage, cover the planting land with film, keep the greenhouse closed, and then seal the greenhouse for 18 days, maintaining the soil moisture content at 75%;

[0072] (7) 5 days after the greenhouse is closed, apply 8 kg / mu of probiotic EM compound agent;

[0073] (8) After cabbage planting

[0074] When the cabbage disease just occurs, dilute the biological agent with water 12 times and spray until water droplets fall off; spray once every 7 days for 2 consecutive times;

[0075] When cabbage pests first occur, dilute the insect repellent 12 times with water and spray until water droplets fall off; spray once every 5 days for 3 consecutive times.

[0076] Example 3

[0077] A method for integrated pest control of vegetables in a solar greenhouse, comprising the following steps:

[0078] (1) Mixing waste mushroom residue, livestock manure, corn straw, soybean meal and wood ash in a mass ratio of 14:55:10:6:7 to obtain base fertilizer;

[0079] The livestock manure includes sheep manure, pig manure and cow manure, with a mass ratio of 60:20:20.

[0080] (2) Bacillus licheniformis, Bacillus amyloliquefaciens, Trichoderma viride, Beauveria bassiana, and Rhodotorula were mixed in a mass ratio of 10:8:10:5:4 to obtain a biological agent;

[0081] The bacterial content of the Bacillus licheniformis is 600 million / g; the bacterial content of the Bacillus amyloliquefaciens is 500 million / g; the bacterial content of the Trichoderma viride is 500 million / g; the bacterial content of the Beauveria bassiana is 300 million / g; and the bacterial content of the red yeast is 300 million / g.

[0082] (3) oleander, cnidium monnieri, pulsatilla striata, chinaberry bark, sophora flavescens and astragalus were mixed with water at a mass ratio of 1:4 and decocted for 3 h, and the residue was removed to obtain oleander extract, cnidium monnieri extract, pulsatilla striata extract, chinaberry bark extract, sophora flavescens extract and astragalus extract, and the different Chinese herbal extracts were mixed to obtain a Chinese herbal extract;

[0083] (4) mixing the Chinese herbal extract with 400 times of 5% emulsifiable concentrate of fluazifop and 600 times of 10% emulsifiable concentrate of cypermethrin to obtain an insect repellent;

[0084] The mass ratio of 400 times diluted 5% emulsifiable concentrate of fluazifop, 600 times diluted 10% emulsifiable concentrate of cypermethrin, oleander extract, cnidium monnieri extract, pulsatilla scabra extract, chinaberry bark extract, sophora flavescens extract and astragalus extract is 4:6:7:14:11:10:11:15;

[0085] (5) Before planting lettuce, apply 350 kg / mu of base fertilizer and then perform rotary tillage to a depth of 15 cm;

[0086] (6) After rotary tillage, cover the planting land with film, keep the greenhouse closed, and then seal the greenhouse for 15 days, maintaining the soil moisture content at 70%;

[0087] (7) 4 days after the greenhouse is closed, apply 6 kg / mu of probiotic EM compound agent;

[0088] (8) After planting lettuce

[0089] When the lettuce disease just occurs, dilute the biological agent with water 10 times and spray it until water droplets fall off; spray once every 7 days for 2 consecutive times;

[0090] When lettuce pests first occur, dilute the insect repellent 10 times with water and spray until water droplets fall off; do this once every 7 days for 2 consecutive times.

[0091] Experimental Example 1

[0092] Comparative test:

[0093] The method of Example 1 was used as experimental group 1;

[0094] At the same time, the following control groups were set up to explore the effects of the shed treatment on vegetable yield and pest and disease infection rate;

[0095] Control group 1: other methods were the same as those in Example 1, except that no suffocation treatment was performed;

[0096] At the same time, the following control groups were set up to explore the effects of base fertilizer on vegetable yield and pest and disease infection rate;

[0097] Control group 2: Other methods were the same as those in Example 1, except that the base fertilizer was replaced with 600 kg / mu of pig manure and 100 kg / mu of compound fertilizer (15-15-15);

[0098] At the same time, the following control groups were set up to explore the effects of biological agents on vegetable yield and pest and disease infection rates;

[0099] Control group 3: other methods were the same as those in Example 1, except that Trichoderma viride was not added;

[0100] Control group 4: other methods were the same as those in Example 1, except that Bacillus licheniformis was not added;

[0101] At the same time, the following control groups were set up to explore the effects of insecticides on vegetable yield and pest infection rates;

[0102] Control group 5: The other methods were the same as those in Example 1, except that no oleander extract was added;

[0103] Control group 6: Other methods were the same as those in Example 1, except that the insect repellent was prepared by mixing chlorfluazuron and cypermethrin, without adding any traditional Chinese medicine components;

[0104] Control group 7: The other methods were the same as those in Example 1, except that the insect repellent was prepared only from traditional Chinese medicine components, without the addition of chlorfluazuron and cypermethrin;

[0105] At the same time, the following control groups were set up to explore the effects of probiotics on vegetable yield and pest and disease infection rates;

[0106] Control group 8: Other methods were the same as those in Example 1, except that no EM compound bacterial agent was added.

[0107] Prior art group 1: Other methods are the same as those in Example 1, with the only difference being that the biological agent and the insecticide are replaced by the product obtained in Example 4 of CN200910196641.8, a microbial preparation capable of reducing leafy vegetable diseases and pests and its preparation method. When used, the product is diluted 200 times and sprayed in a mist form on the stems and leaves of the plant. Both sides of the leaves should be sprayed, and each spraying is performed until water droplets slide off.

[0108] The infection rates of Spodoptera litura and white rust were investigated during the water spinach planting period. After the water spinach was harvested, the yield of water spinach was measured. The results are shown in Table 1.

[0109] Table 1 Water spinach survey results

[0110] Yield per mu kg Spodoptera litura infection rate (%) White rust infection rate % Experimental Group 1 4565.4 10.2 9.3 Control group 1 3687.5 20.5 19.6 Control group 2 4123.4 11.5 14.6 Control group 3 4231.4 13.2 22.3 Control group 4 4287.6 12.5 18.7 Control group 5 4068.4 15.2 11.6 Control group 6 3775.1 21.3 10.8 Control group 7 3725.0 24.6 10.4 Control group 8 4321.4 15.5 18.7 Prior Art Group 1 3946.7 15.7 21.6

[0111] It can be seen from the contents recorded in Table 1 that the method of the present invention can significantly increase the yield of water spinach, and compared with the control method of the prior art group, the control method of the present invention can effectively reduce the incidence of diseases and pests of water spinach, thereby increasing the yield of water spinach.

[0112] The data from control group 1 show that if the greenhouse is not sealed, pathogens or pests lurking in the soil cannot be suppressed, resulting in large-scale outbreaks of vegetable diseases and pests after vegetable planting, which are difficult to effectively control even with subsequent drug control. However, the greenhouse sealing treatment of the present invention effectively kills pathogens or pests before vegetable planting.

[0113] It can be seen from the data of control group 2 that if the base fertilizer used in the present invention is replaced with other types of base fertilizers, the effect is not as good as that of the present invention. The base fertilizer used in the present invention has a better growth-promoting effect on the growth of vegetables.

[0114] From the data of control groups 3 and 4, it can be seen that the biological agent prepared by the present invention has an excellent control effect on vegetable diseases. The beneficial bacteria act synergistically. If some components are discarded, the balance between the species will be broken and the original effect will not be achieved.

[0115] The insect repellent provided by the present invention is a combination of chemical agents and traditional Chinese medicine preparations, which have a synergistic insect repellent effect. From the data of control groups 5, 6, and 7, it can be seen that if the chemical agents or traditional Chinese medicine preparations are abandoned, the incidence of vegetable insect pests is significantly increased.

[0116] After the greenhouse is sealed, the application of EM microbial agents can enable beneficial bacteria to colonize in the soil as quickly as possible, making the beneficial bacteria the dominant species, thereby preventing the colonization of harmful bacteria. From the data of control group 8, it can be seen that if EM microbial agents are not applied, the effect of vegetable disease and pest control will be significantly worse.

[0117] Experimental Example 2

[0118] Comparative test:

[0119] The method of Example 2 was used as experimental group 2;

[0120] The setting of control groups 1 to 8 and prior art group 1 was the same as that of experimental example 1, and they were named control groups 9 to 16 and prior art group 2.

[0121] The infection rates of lepidopteran pests and soft rot were investigated during the cabbage planting period. After the cabbage was harvested, the yield of the cabbage was measured. The results are shown in Table 2.

[0122] Table 2 Cabbage survey results

[0123]

[0124]

[0125] As can be seen from the contents recorded in Table 2, the control method of the present invention also has excellent control effects on various diseases and insect pests of cabbage. In addition, the method of the present invention can also significantly increase the yield of cabbage and improve the economic benefits of farmers.

[0126] Experimental Example 3

[0127] Comparative test:

[0128] The method of Example 3 was used as experimental group 3;

[0129] The setting of control groups 1 to 8 and prior art group 1 was the same as that of experimental example 1, and they were named control groups 17 to 24 and prior art group 3.

[0130] The infection rates of leafminer and downy mildew were investigated during the lettuce planting period. After the lettuce was harvested, the yield of the lettuce was measured. The results are shown in Table 2.

[0131] Table 3 Lettuce survey results

[0132] Yield per mu kg Leaf miner infection rate (%) Downy mildew infection rate (%) Experimental Group 3 2854.6 12.6 17.5 Control group 17 2587.5 18.4 25.8 Control group 18 2645.6 13.4 22.6 Control group 19 2615.7 13.2 26.2 Control group 20 2601.2 12.9 25.7 Control group 21 2711.3 15.3 19.2 Control group 22 2432.5 19.7 18.2 Control group 23 2732.7 20.3 18.6 Control group 24 2703.3 12.7 18.2 Prior Art Group 3 2554.1 17.5 27.1％

[0133] Based on the contents of Tables 1 to 3, the method of the present invention has a relatively good control effect on various vegetable diseases and insect pests, and can further increase vegetable yields. While there are many control agents for vegetable diseases and insect pests in the prior art, different control agents must be used for different diseases and insect pests. The present invention provides a universal agent for vegetable diseases and insect pests, respectively. The same agent can be used for different types of diseases and insect pests, which facilitates the management process of farmers. Moreover, the use of the control agent of the present invention does not cause the problem of excessive pesticide residues.

[0134] The above is only a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principles of the present invention. These improvements and modifications should also be regarded as within the scope of protection of the present invention.

Claims

1. A method for integrated pest control of greenhouse vegetables, characterized in that: The steps include: (1) Before planting vegetables, apply base fertilizer, till the soil, cover with film, seal the greenhouse, and apply probiotics; (2) After vegetable planting When the disease occurs, spraying biological agents: the biological agents include: Bacillus licheniformis, Bacillus amyloliquefaciens, Trichoderma viride, Beauveria bassiana and Rhodotorula; When pests occur, spraying insecticides; the insecticides include: chlorfluazuron, cypermethrin, oleander extract, cnidium monnieri extract, pulsatilla extract, chinaberry bark extract, sophora flavescens extract and astragalus extract; The base fertilizer in step (1) comprises: waste mushroom residue, livestock excrement, straw, soybean meal and wood ash, with a mass ratio of 12-16:45-65:8-12:4-8:5-9; The probiotics in step (1) are EM compound bacteria; the application amount of the EM compound bacteria is 4-8 kg / mu; In step (2), the bacterial content of the Bacillus licheniformis is 400-800 million / g; the bacterial content of the Bacillus amyloliquefaciens is 300-700 million / g; the bacterial content of the Trichoderma viride is 400-600 million / g; the bacterial content of the Beauveria bassiana is 200-400 million / g; and the bacterial content of the red yeast is 200-400 million / g. The mass ratio of the Bacillus licheniformis, Bacillus amyloliquefaciens, Trichoderma viride, Beauveria bassiana and Rhodotorula is 8-12:6-10:8-12:4-6:3-5; The mass ratio of chlorfluazuron, cypermethrin, oleander extract, cnidium monnieri extract, pulsatilla scabra extract, chinaberry bark extract, sophora flavescens extract and astragalus extract in step (2) is 2-6:4-8:5-9:12-16:7-13:8-12:9-13:13-17.

2. A method for integrated pest control of greenhouse vegetables according to claim 1, characterized in that: The depth of the rotary tillage in step (1) is 10-20 cm.

3. The method for integrated pest control of greenhouse vegetables according to claim 1, characterized in that: The time of the sealed greenhouse in step (1) is 12 to 18 days; the soil moisture content is maintained at 65 to 75% during the sealed greenhouse.

4. The method for integrated pest control of greenhouse vegetables according to claim 1, characterized in that: Step (1) Apply probiotics within 3 to 5 days after the end of the greenhouse.

5. The method for integrated pest control of greenhouse vegetables according to claim 1, characterized in that: The vegetables are lettuce, cabbage or water spinach.

Images