Anaerobic ammonia oxidation sludge storage device and method

Abstract

The application discloses an anaerobic ammonia oxidation sludge storage device, which comprises a storage liquid adjusting area, a sludge storage area and a waste liquid disposal area. The storage liquid adjusting area comprises a substrate liquid storage tank, an alkali liquid storage tank and an acid liquid storage tank. The sludge storage area comprises a sludge storage tank. The waste liquid disposal area comprises a waste liquid storage tank. The sludge storage tank is provided with a temperature control device, a water quality monitoring integrated device and a stirrer. The temperature control device has temperature sensing, heating and refrigeration functions. The application also discloses an anaerobic ammonia oxidation sludge storage method. Through the setting of the storage liquid adjusting area, the sludge storage area and the waste liquid disposal area, real-time monitoring, adjustment and replacement of anaerobic ammonia oxidation bacteria storage liquid can be realized economically and effectively.

Description

Technical Field

[0001] This invention relates to the field of wastewater treatment technology, specifically to an anaerobic ammonia oxidation sludge storage device and method. Background Technology

[0002] Anaerobic ammonia oxidation (AAO) is a process that uses nitrite as an electron acceptor under anaerobic conditions to oxidize ammonia nitrogen into nitrogen gas, achieving the simultaneous removal of both nitrogen pollutants. Compared to traditional biological nitrogen removal processes, AAO has advantages such as better nitrogen removal efficiency, no need for external organic carbon sources, and lower operating costs, making it a promising technology for industrial applications.

[0003] Anaerobic ammonia oxidizing bacteria have a long doubling time and slow growth, making pure culture difficult and thus hindering their application in practical wastewater treatment. Enriching and effectively storing anaerobic ammonia oxidizing bacteria is an effective measure to address their limited application. Furthermore, in actual operation, the seasonality of certain industrial wastewater and rural sewage sources may lead to the shutdown of anaerobic ammonia oxidation processes. In all these processes, the storage of anaerobic ammonia oxidation sludge is an unavoidable technical issue. Summary of the Invention

[0004] In view of the existing technical problems, the present invention provides an anaerobic ammonia oxidation sludge storage device and method to solve the problems in the prior art.

[0005] To achieve the above-mentioned objectives, the present invention provides the following technical solution:

[0006] An anaerobic ammonia oxidation sludge storage device includes a storage liquid conditioning zone, a sludge storage zone, and a waste liquid disposal zone. The storage liquid conditioning zone includes a substrate liquid storage tank, an alkali liquid storage tank, and an acid liquid storage tank. The sludge storage zone includes a sludge storage tank, and the waste liquid disposal zone includes a waste liquid storage tank. The substrate liquid storage tank and the sludge storage tank are connected via a first pipeline. The alkali liquid storage tank and the sludge storage tank are connected via a second pipeline. The acid liquid storage tank and the sludge storage tank are connected via a third pipeline. The sludge storage tank and the waste liquid storage tank are connected via a fourth pipeline. The sludge storage tank is equipped with a temperature control device, an integrated water quality monitoring device, and a stirrer. The temperature control device has temperature sensing and heating / cooling functions. A first transfer pump, a second transfer pump, a third transfer pump, and a fourth transfer pump are respectively installed on the first, second, third, and fourth pipelines.

[0007] Preferably, it also includes an automated control area, which is equipped with a PLC and a computer. The temperature control device, the integrated water quality monitoring device, the stirrer, the first delivery pump, the second delivery pump, the third delivery pump and the fourth delivery pump are respectively electrically connected to the PLC, and the PLC and the computer are electrically connected.

[0008] Preferably, the sludge storage tank includes a tank body and a tank cover, the tank body and the tank cover are detachably connected, and the tank body and the tank cover are covered with an insulation layer.

[0009] Preferably, the stirrer is a stirring paddle or a magnetic stir bar.

[0010] Preferably, the matrix solution in the matrix solution storage tank is a mixed solution of nitrate and glucose, with a nitrate concentration of 70-140 mg-N / L, a glucose concentration of 1-2 mM, and a pH value of 7.0-8.0.

[0011] Preferably, the alkaline solution in the alkaline solution storage tank is a sodium hydroxide solution with a concentration of 1-3 mol / L.

[0012] Preferably, the acid in the acid storage tank is a hydrochloric acid solution with a concentration of 1-3 mol / L.

[0013] A method for storing anaerobic ammonia oxidation sludge, the method using the above-mentioned anaerobic ammonia oxidation sludge storage device, includes the following process:

[0014] (a) Water quality monitoring

[0015] The integrated water quality monitoring equipment monitors the pH value and the concentrations of ammonia nitrogen, nitrite, nitrate, and glucose in the sludge storage tank in real time. If the pH deviates from the control range, an acid-base adjustment program is initiated. If the concentrations of ammonia nitrogen and nitrite exceed the control range or the concentrations of nitrate and glucose are lower than the control values, a storage liquid replacement program is initiated. The temperature control equipment monitors the temperature of the storage liquid in the sludge storage tank in real time. If the temperature deviates from the control value, a temperature adjustment program is initiated.

[0016] (b) Acid-base adjustment procedure

[0017] The pH value of the storage liquid in the sludge storage tank is controlled between 7.0 and 8.0. When the pH value deviates from the control range, acid-base adjustment is initiated: if the pH value of the storage liquid is lower than 7.0, the second transfer pump is activated to pump alkaline solution from the alkaline solution storage tank to the sludge storage tank, so that the pH value of the storage liquid is within the range of 7.0-8.0; if the pH value of the storage liquid is higher than 8.0, the third transfer pump is activated to pump acid solution from the acid solution storage tank to the sludge storage tank, so that the pH value of the storage liquid is within the range of 7.0-8.0.

[0018] (c) Storage fluid replacement procedure

[0019] The nitrate concentration of the storage liquid in the sludge storage tank is controlled above 20 mg-N / L, the glucose concentration is controlled above 0.2 mM, and the ammonia nitrogen and nitrite concentrations are both controlled below 60-70 mg-N / L. If the ammonia nitrogen and nitrite concentrations of the storage liquid exceed the control range or the nitrate and glucose concentrations of the storage liquid are lower than the control values, the storage liquid replacement procedure is initiated: first, the waste liquid disposal procedure is initiated, and the storage liquid is pumped from the sludge storage tank to the waste liquid storage tank through the fourth transfer pump; then, the matrix liquid replenishment procedure is initiated, and the matrix liquid is pumped from the matrix liquid storage tank to the sludge storage tank through the first transfer pump. The nitrate concentration of the matrix liquid is 70-140 mg-N / L, the glucose concentration is 1-2 mM, and the pH value is 7.0-8.0.

[0020] (d) Temperature control program

[0021] The temperature of the storage liquid in the sludge storage tank is controlled at 4°C. If the temperature deviates from the control value, the temperature control device will activate the heating or cooling function to adjust the temperature of the storage liquid back to the control temperature.

[0022] Compared with the prior art, the beneficial effects of the present invention are as follows: By setting up a storage liquid adjustment zone, a sludge storage zone, and a waste liquid disposal zone, this solution can economically and effectively realize the real-time monitoring, adjustment, and replacement of the anaerobic ammonia oxidation bacteria storage liquid; it can lay an important foundation for the construction of an anaerobic ammonia oxidation sludge bacteria resource bank, and at the same time provide a source of bacteria for the industrial application of anaerobic ammonia oxidation process. Attached Figure Description

[0023] Figure 1 This is a schematic diagram of the anaerobic ammonia oxidation sludge storage device of the present invention. Detailed Implementation

[0024] The present invention will be further described in detail below with reference to experimental examples and specific embodiments. However, this should not be construed as limiting the scope of the above-mentioned subject matter of the present invention to the following embodiments; all technologies implemented based on the content of the present invention fall within the scope of the present invention.

[0025] In the description of this invention, it should be understood that the terms "longitudinal", "lateral", "up", "down", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are only for the convenience of describing this invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this invention.

[0026] In the description of this invention, unless otherwise specified and limited, it should be noted that the terms "installation", "connection" and "linking" should be interpreted broadly. For example, they can refer to mechanical or electrical connections, or internal connections between two components. They can be direct connections or indirect connections through an intermediate medium. Those skilled in the art can understand the specific meaning of the above terms according to the specific circumstances.

[0027] As attached Figure 1 The anaerobic ammonia oxidation sludge storage device shown includes a storage liquid conditioning zone, a sludge storage zone, and a waste liquid disposal zone. The storage liquid conditioning zone includes a substrate liquid storage tank 11, an alkali liquid storage tank 12, and an acid liquid storage tank 13. The sludge storage zone includes a sludge storage tank 21, and the waste liquid disposal zone includes a waste liquid storage tank 31. The sludge storage tank 21 includes a tank body and a tank cover, which are detachably connected, such as by threaded connections or clamp connections. The tank body and tank cover are externally covered with an insulation layer 23. The substrate liquid in the substrate liquid storage tank 11 is a mixed solution of nitrate and glucose, with a nitrate concentration of 70-140 mg-N / L, a glucose concentration of 1-2 mM, and a pH value of 7.0-8.0. The alkali liquid in the alkali liquid storage tank 12 is a sodium hydroxide solution with a concentration of 1-3 mol / L. The acid liquid in the acid liquid storage tank 13 is a hydrochloric acid solution with a concentration of 1-3 mol / L.

[0028] The substrate liquid storage tank 11 is connected to the sludge storage tank 21 via a first pipe 17; the alkali liquid storage tank 12 is connected to the sludge storage tank 21 via a second pipe 18; the acid liquid storage tank 13 is connected to the sludge storage tank 21 via a third pipe 19; and the sludge storage tank 21 and the waste liquid storage tank 31 are connected via a fourth pipe 33. The sludge storage tank 21 is equipped with a temperature control device 24, an integrated water quality monitoring device 25, and a stirrer 26. The stirrer 26 is either a stirring paddle or a magnetic stir bar. The integrated water quality monitoring device 25 detects pH value, ammonia nitrogen concentration, nitrite concentration, nitrate concentration, and glucose concentration. The temperature control device 24 has temperature sensing and heating / cooling functions. A first transfer pump 14, a second transfer pump 15, a third transfer pump 16, and a fourth transfer pump 32 are respectively installed on the first pipe 17, the second pipe 18, the third pipe 19, and the fourth pipe 33.

[0029] The end of the first pipe 17 connected to the matrix liquid storage tank 11 is close to the bottom of the matrix liquid storage tank 11; the end of the second pipe 18 connected to the alkali liquid storage tank 12 is close to the bottom of the alkali liquid storage tank 12; and the end of the third pipe 19 connected to the acid liquid storage tank 13 is close to the bottom of the acid liquid storage tank 13.

[0030] It also includes an automated control area, which is equipped with a PLC41 and a computer42. Temperature control device 24, integrated water quality monitoring device 25, stirrer 26, first delivery pump 14, second delivery pump 15, third delivery pump 16 and fourth delivery pump 32 are electrically connected to the PLC41, and the PLC41 and computer42 are electrically connected.

[0031] A method for storing anaerobic ammonia oxidation sludge, the method using the aforementioned anaerobic ammonia oxidation sludge storage device, includes the following process:

[0032] (a) Water quality monitoring

[0033] The integrated water quality monitoring device 25 monitors the pH value and the concentrations of ammonia nitrogen, nitrite, nitrate, and glucose in the storage liquid in the sludge storage tank 21 in real time; if the pH value deviates from the control range, the acid-base adjustment program is initiated; if the concentrations of ammonia nitrogen and nitrite exceed the control range or the concentrations of nitrate and glucose are lower than the control values, the storage liquid replacement program is initiated; the temperature control device 24 monitors the temperature of the storage liquid in the sludge storage tank 21 in real time, and if the temperature deviates from the control value, the temperature adjustment program is initiated.

[0034] (b) Acid-base adjustment procedure

[0035] The pH value of the storage liquid in the sludge storage tank 21 is controlled between 7.0 and 8.0. When the pH value deviates from the control range, acid-base adjustment is initiated: if the pH value of the storage liquid is lower than 7.0, the second transfer pump 15 is activated to pump alkaline solution from the alkaline solution storage tank 12 to the sludge storage tank 21, so that the pH value of the storage liquid is within the range of 7.0-8.0; if the pH value of the storage liquid is higher than 8.0, the third transfer pump 16 is activated to pump acid solution from the acid solution storage tank 13 to the sludge storage tank 21, so that the pH value of the storage liquid is within the range of 7.0-8.0.

[0036] (c) Storage fluid replacement procedure

[0037] The nitrate concentration of the storage liquid in the sludge storage tank 21 is controlled above 20 mg-N / L, the glucose concentration is controlled above 0.2 mM, and the ammonia nitrogen and nitrite concentrations are both controlled below 60-70 mg-N / L. If the ammonia nitrogen and nitrite concentrations of the storage liquid exceed the control range or the nitrate and glucose concentrations of the storage liquid are lower than the control values, the storage liquid replacement procedure is initiated: first, the waste liquid disposal procedure is initiated, and the storage liquid is pumped from the sludge storage tank 21 to the waste liquid storage tank 31 through the fourth transfer pump 32; then, the matrix liquid replenishment procedure is initiated, and the matrix liquid is pumped from the matrix liquid storage tank 11 to the sludge storage tank 21 through the first transfer pump 14. The nitrate concentration of the matrix liquid is 70-140 mg-N / L, the glucose concentration is 1-2 mM, and the pH value is 7.0-8.0.

[0038] (d) Temperature control program

[0039] The temperature of the storage liquid in the sludge storage tank 21 is controlled at 4°C. If the temperature deviates from the control value, the temperature control device 24 will start the heating or cooling function to adjust the temperature of the storage liquid back to the control temperature.

[0040] Anaerobic ammonia oxidation (Anammox) is an economical, efficient, and environmentally friendly biological nitrogen removal technology, particularly suitable for treating wastewater with low organic carbon and high ammonia nitrogen. Specifically, Anammox is a biological process under anoxic conditions, using ammonia as an electron donor and nitrite as an electron acceptor to generate nitrogen gas and some nitrate nitrogen. Anaerobic ammonia oxidizing bacteria are extremely sensitive to nitrite, which is the main cause of substrate inhibition in these bacteria. Furthermore, these bacteria, the functional microorganisms in the above biological process, can also utilize various organic or inorganic electron donors to perform the dissimilatory nitrate reduction to ammonium (DNRA) reaction. DNRA, under anoxic conditions, uses nitrate nitrogen as an electron acceptor and nitrite as an intermediate product, ultimately producing ammonia nitrogen. The ammonia nitrogen and nitrite produced in this biological process can be utilized by anaerobic ammonia oxidizing bacteria to ultimately generate nitrogen gas, i.e., the dissimilatory nitrate reduction to ammonia coupled with anaerobic ammonia oxidation (DNRA-Anammox) reaction. Because nitrite is produced and consumed simultaneously during the reaction, the DNRA-Anammox reaction, which uses nitrate as an electron acceptor, is less prone to matrix inhibition compared to the traditional Anammox reaction, which uses ammonia nitrogen and nitrite as the matrix. This makes it particularly suitable for the low-temperature preservation of anaerobic ammonia-oxidizing bacteria.

[0041] Using the apparatus and method described in this invention, anammox sludge was stored for 118 days, and the specific activity of anammox (SAA) retention rate of the anammox sludge was as high as 92.7%. Obviously, the storage apparatus and method provided by this invention are effective in the long term.

[0042] The preferred embodiments of the present invention have been described above. It should be understood that those skilled in the art can make numerous modifications and variations based on the concept of the present invention without creative effort. Therefore, all technical solutions that can be obtained by those skilled in the art based on the concept of the present invention through logical analysis, reasoning, or limited experimentation on the basis of existing technology should be within the scope of protection defined by the claims.

Claims

1. A method for storing anaerobic ammonia oxidation sludge, characterized in that, The sludge storage method employs a storage device comprising a storage liquid conditioning zone, a sludge storage zone, and a waste liquid disposal zone. The storage liquid conditioning zone includes a substrate liquid storage tank (11), an alkali liquid storage tank (12), and an acid liquid storage tank (13). The sludge storage zone includes a sludge storage tank (21), and the waste liquid disposal zone includes a waste liquid storage tank (31). The substrate liquid storage tank (11) and the sludge storage tank (21) are connected via a first pipe (17). The alkali liquid storage tank (12) and the sludge storage tank (21) are connected via a second pipe (18). The acid liquid storage tank (13) and the sludge storage tank (21) are connected via a second pipe (18). The sludge storage tank (21) and the waste liquid storage tank (31) are connected by a third pipe (19), and the sludge storage tank (21) and the waste liquid storage tank (31) are connected by a fourth pipe (33). The sludge storage tank (21) is equipped with a temperature control device (24), an integrated water quality monitoring device (25) and a stirrer (26). The temperature control device (24) has temperature sensing and heating and cooling functions. The first pipe (17), the second pipe (18), the third pipe (19) and the fourth pipe (33) are respectively equipped with a first delivery pump (14), a second delivery pump (15), a third delivery pump (16) and a fourth delivery pump (32); This method includes the following steps: (a) Water quality monitoring The integrated water quality monitoring device (25) monitors the pH value and the concentrations of ammonia nitrogen, nitrite, nitrate and glucose in the sludge storage tank (21) in real time; if the pH deviates from the control range, the acid-base adjustment program is started; if the concentrations of ammonia nitrogen and nitrite exceed the control range or the concentrations of nitrate and glucose are lower than the control values, the storage liquid replacement program is started; the temperature control device (24) monitors the temperature of the storage liquid in the sludge storage tank (21) in real time; if the temperature deviates from the control value, the temperature adjustment program is started. (b) Acid-base adjustment procedure The pH value of the storage liquid in the sludge storage tank (21) is controlled between 7.0 and 8.

0. When the pH value deviates from the control range, acid-base adjustment is initiated: if the pH value of the storage liquid is lower than 7.0, the second transfer pump (15) is started to pump the alkaline solution from the alkaline solution storage tank (12) to the sludge storage tank (21) so that the pH value of the storage liquid is in the range of 7.0-8.0; if the pH value of the storage liquid is higher than 8.0, the third transfer pump (16) is started to pump the acid solution from the acid solution storage tank (13) to the sludge storage tank (21) so that the pH value of the storage liquid is in the range of 7.0-8.

0. (c) Storage fluid replacement procedure The nitrate concentration in the sludge storage tank (21) is controlled above 20 mg-N / L, the glucose concentration is controlled above 0.2 mM, and the ammonia nitrogen and nitrite concentrations are controlled below 60-70 mg-N / L. If the ammonia nitrogen and nitrite concentrations in the storage liquid exceed the control range or the nitrate and glucose concentrations in the storage liquid are lower than the control values, the storage liquid replacement procedure is initiated: first, the waste liquid disposal procedure is initiated, and the storage liquid is pumped from the sludge storage tank (21) to the waste liquid storage tank (31) through the fourth transfer pump (32). Then, the matrix liquid replenishment procedure is initiated, and the matrix liquid is pumped from the matrix liquid storage tank (11) to the sludge storage tank (21) through the first transfer pump (14). The matrix solution in the matrix solution storage tank (11) is a mixed solution of nitrate and glucose, with a nitrate concentration of 70-140 mg-N / L, a glucose concentration of 1-2 mM, and a pH value of 7.0-8.

0. (d) Temperature control program The temperature of the storage liquid in the sludge storage tank (21) is controlled at 4°C. If the temperature deviates from the control value, the temperature control device (24) will start the heating or cooling function to adjust the temperature of the storage liquid back to the control temperature.

2. The method for storing anaerobic ammonia oxidation sludge according to claim 1, characterized in that: It also includes an automated control area, which is equipped with a PLC (41) and a computer (42). The temperature control device (24), the integrated water quality monitoring device (25), the stirrer (26), the first delivery pump (14), the second delivery pump (15), the third delivery pump (16) and the fourth delivery pump (32) are electrically connected to the PLC (41), and the PLC (41) and the computer (42) are electrically connected.

3. The method for storing anaerobic ammonia oxidation sludge according to claim 1, characterized in that: The sludge storage tank (21) includes a tank body and a tank cover, the tank body and the tank cover are detachably connected, and the tank body and the tank cover are covered with an insulation layer (23).

4. The method for storing anaerobic ammonia oxidation sludge according to claim 3, characterized in that: The stirrer (26) is a stirring paddle or a magnetic stir bar.

5. A method for storing anaerobic ammonia oxidation sludge according to claim 4, characterized in that: The alkaline solution in the alkaline storage tank (12) is a sodium hydroxide solution with a concentration of 1-3 mol / L.

6. A method for storing anaerobic ammonia oxidation sludge according to claim 5, characterized in that: The acid in the acid storage tank (13) is a hydrochloric acid solution with a concentration of 1-3 mol / L.

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