A pet sterilizer and deodorant

Abstract

The present invention relates to a pet sterilizer and deodorizer, which belongs to the technical field of pet deodorization. The present invention promotes the long-term expression of hop extract and enhances the sterilization and deodorization effect through the synergistic effect of hop extract, persimmon tannin, and lysozyme. Furthermore, the composition is stable, natural, and pollution-free, and can be used in the oral cavity and in vitro of animals. The present invention also provides zinc ricinoleate and an antibacterial agent. Through the three-phase interaction of zinc ricinoleate, hop extract, and antibacterial agent, the overall stability is enhanced and the antibacterial time of the complex is prolonged while ensuring antibacterial and deodorizing effects. The present invention also provides a modified quaternary ammonium salt antibacterial liquid. The modified quaternary ammonium salt has enhanced stability and, when present together with zinc ricinoleate, promotes an enhanced deodorizing effect. The present invention also provides an Artemisia annua extract, a Gentiana root extract, and an Artemisia argyi extract, which can eliminate odor sources and significantly inhibit bacterial growth, thereby having significant antibacterial, deodorizing, and odor-removing effects.

Description

Technical Field

[0001] The present invention belongs to the technical field of pet deodorization, and in particular relates to a pet sterilizing and deodorizing agent. Background Art

[0002] Pet urine, feces, saliva, and anal glands produce odors that pollute the home environment. These odorous gases are primarily multi-component, low-boiling-point, small-molecule mixtures, primarily including ammonia, hydrogen sulfide, indole, and skatole. These odorous gases are volatile, have a low perception threshold, and exhibit a non-linear relationship between perception intensity and concentration. Prolonged exposure to odorous gases can be harmful to human health, affecting the respiratory, digestive, cardiovascular, and endocrine systems. This is why pet deodorizing sprays have emerged.

[0003] Existing deodorization technologies include chemical deodorants, fragrance masking technology, physical deodorization technology, antibacterial and antimicrobial technology, and biological deodorization technology.

[0004] Common chemical deodorants remove odors through chemical reactions and bonding with odor molecules. For example, zinc ricinoleate, with its activated Zn ion structure, can irreversibly bond with a variety of odor molecules, achieving a deodorizing effect.

[0005] Ordinary fragrance masking technology is not enough to remove pet odors, and it is easy to stimulate the pet's sense of smell (a pet's sense of smell is 400,000 to 1,000,000 times that of humans), which has adverse effects on pet health.

[0006] Physical deodorization refers to the use of certain adsorption substrates to bind odor molecules, thereby achieving the effect of removing odor. Physical deodorization is short-lived and cannot completely eliminate odors.

[0007] Pets often carry bacteria and fungi, and if their excrement is not cleaned up promptly, it is more likely to breed microorganisms. The addition of antibacterial and antimicrobial technologies can eliminate odors by inhibiting the growth of microorganisms.

[0008] Biological deodorants typically use enzymes produced by microbial metabolism or by adding enzymes directly to degrade organic matter that causes unpleasant odors, thereby controlling or inhibiting the production of odors. It's important to note that biological enzymes are specific, meaning they react chemically to only one type of substance.

[0009] Chemical deodorants and antibacterial and antimicrobial deodorization technologies offer long-term effectiveness. However, when using existing deodorants, safety and irritation must be considered in addition to effectiveness. Furthermore, the addition of antimicrobial and antimicrobial agents has little effect on existing odors and odors from non-bacterial sources. Summary of the Invention

[0010] The present invention relates to a pet sterilizer and deodorizer, which belongs to the technical field of pet deodorization. The present invention promotes the long-term expression of hop extract and enhances the sterilization and deodorization effect through the synergistic effect of hop extract, persimmon tannin, and lysozyme. Furthermore, the composition is stable, natural, and pollution-free, and can be used in the oral cavity and in vitro of animals. The present invention also provides zinc ricinoleate and an antibacterial agent. Through the three-phase interaction of zinc ricinoleate, hop extract, and antibacterial agent, the overall stability is enhanced and the antibacterial time of the complex is prolonged while ensuring antibacterial and deodorizing effects. The present invention also provides a modified quaternary ammonium salt antibacterial liquid. The modified quaternary ammonium salt has enhanced stability and, when present together with zinc ricinoleate, promotes an enhanced deodorizing effect. The present invention also provides an Artemisia annua extract, a Gentiana root extract, and an Artemisia argyi extract, which can eliminate odor sources and significantly inhibit bacterial growth, thereby having significant antibacterial, deodorizing, and odor-removing effects.

[0011] The purpose of the present invention can be achieved through the following technical solutions:

[0012] A pet sterilizer and deodorizer, comprising the following raw materials in the following mass percentages:

[0013] Hops extract 0.05-0.2%, stabilizer 0.5-3%, citric acid 0.1-1%, persimmon tannin 0.05%-0.5%, lysozyme 0.05%-0.5%, and the balance is deionized water.

[0014] As a preferred embodiment of the present invention, the contents of α-acid and β-acid in the hop extract range from 400-600 mg / g and 100-300 mg / g, respectively.

[0015] As a preferred embodiment of the present invention, the stabilizer is at least one of acetate buffer and glycerol.

[0016] As a preferred embodiment of the present invention, the pet sterilizer and deodorizer further comprises 1-3% of an antibacterial agent and 3-4% of zinc ricinoleate.

[0017] As a preferred embodiment of the present invention, the antibacterial agent is a modified quaternary ammonium salt antibacterial liquid, which is prepared by the following operations:

[0018] (1) Chitosan was dissolved in an ethanol solution with a volume concentration of 50%, and then a glacial acetic acid solution with a mass concentration of 30% was added dropwise to form a mixture A;

[0019] (2) Add 50% glutaraldehyde solution to mixture A and stir at room temperature for 60-90 minutes to form mixture B;

[0020] (3) adding sodium triacetoxyborohydride into dichloroethane to dissolve the mixture to prepare a mixture C;

[0021] (4) Add mixture B to mixture C, stir for 90-120 min, then add N-bromosuccinimide and continue stirring for 90-120 min to obtain a modified quaternary ammonium salt antibacterial liquid.

[0022] As a preferred embodiment of the present invention, the ratio of chitosan, ethanol solution and glacial acetic acid solution in (1) is 1 g: 180-200 mL: 1-2 mL.

[0023] As a preferred embodiment of the present invention, the amount of the glutaraldehyde solution added in (2) is controlled so that the molar ratio of glutaraldehyde to -NH2 is 1:1.5-2.

[0024] As a preferred embodiment of the present invention, the ratio of sodium triacetoxyborohydride to dichloroethane in (3) is 1 g:40-50 mL.

[0025] As a preferred embodiment of the present invention, the mass ratio of the amount of chitosan added in the mixture B described in (4) to the amount of sodium triacetoxyborohydride in the mixture C is controlled to be 1:3-5, and the mass ratio of the N-bromosuccinimide to the chitosan described in (1) is controlled to be 0.8-1.2:1.

[0026] As a preferred embodiment of the present invention, the pet sterilizer and deodorant further comprises the following raw materials in the following mass percentages: 2-5% of Artemisia annua extract, 3-4% of Gentiana root extract, and 3-4% of Artemisia argyi extract.

[0027] Beneficial effects of the present invention:

[0028] 1. The present invention provides a pet sterilizer and deodorizer. Through the synergistic effect of hop extract, persimmon tannin and lysozyme, while maintaining the good sterilization effect of lysozyme, a bond mainly composed of hydrogen bonds and van der Waals forces is generated between persimmon tannin and lysozyme to form a stable structure. This stable structure promotes the long-term expression of hop extract and enhances the sterilization and deodorization effect. In addition, the composition is stable, natural and pollution-free, and can be used in the oral cavity of animals and in vitro.

[0029] 2. The present invention also provides zinc ricinoleate and an antibacterial agent. Through the three-phase interaction of zinc ricinoleate, hop extract and antibacterial agent, the overall stability is enhanced and the antibacterial time of the complex is prolonged while ensuring antibacterial and deodorizing effects.

[0030] 3. The hop extract of the present invention has a good in vitro antibacterial effect. When combined with zinc ricinoleate within the scope provided by the present invention, it can remove odor while having a good antibacterial effect. At the same time, zinc ricinoleate has a protective effect on hop acid and can slow down its oxidative degradation.

[0031] 4. The present invention provides a modified quaternary ammonium salt antibacterial liquid. The quaternary ammonium salt is positively charged in water, generating electrostatic attraction and binding with the negatively charged bacterial cell membrane, disrupting the normal permeability of the cell membrane. At the same time, the hydrophobic long-chain groups of the quaternary ammonium salt will pierce the cell membrane and enter the cell interior, disrupting the normal cell metabolism process. The modified quaternary ammonium salt has enhanced stability and significantly improves the deodorizing effect when present with zinc ricinoleate.

[0032] 5. The present invention also provides Artemisia annua extract, Gentiana root extract and Artemisia argyi extract. In the substances obtained by compounding according to the present invention, the active ingredients, such as sesquiterpenes, coumarins, gentiopicroside, glycyrrhizic acid and flavonoids, react chemically with odor molecules to eliminate the odor source and significantly inhibit bacterial growth, reduce the activity of odor molecules, and reduce the generation and accumulation of odor. Therefore, the substances have significant antibacterial, deodorizing and odor-removing effects. DETAILED DESCRIPTION

[0033] The following will be combined with the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the embodiments described are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making any creative efforts shall fall within the scope of protection of the present invention.

[0034] Example 1

[0035] A pet sterilizer and deodorant (can be used in the oral cavity), comprising the following raw materials in the following mass percentages:

[0036] Hops extract 0.06%, glycerin 0.52%, citric acid 0.11%, persimmon tannin 0.05%, lysozyme 0.05%, and the balance is deionized water.

[0037] The contents of alpha-acid and beta-acid in the hop extract are respectively in the range of 400-600 mg / g and 100-300 mg / g.

[0038] Example 2

[0039] A pet sterilizer and deodorant (can be used in the oral cavity), comprising the following raw materials in the following mass percentages:

[0040] Hops extract 0.14%, glycerol 1.67%, citric acid 0.54%, persimmon tannin 0.26%, lysozyme 0.25%, and the balance is deionized water.

[0041] The contents of alpha-acid and beta-acid in the hop extract are respectively in the range of 400-600 mg / g and 100-300 mg / g.

[0042] Example 3

[0043] A pet sterilizer and deodorant (can be used in the oral cavity), comprising the following raw materials in the following mass percentages:

[0044] Hops extract 0.19%, glycerin 3%, citric acid 0.99%, persimmon tannin 0.5%, lysozyme 0.5%, and the balance is deionized water.

[0045] The contents of alpha-acid and beta-acid in the hop extract are respectively in the range of 400-600 mg / g and 100-300 mg / g.

[0046] Example 4

[0047] A pet sterilizer and deodorant (can be used in the oral cavity), comprising the following raw materials in the following mass percentages:

[0048] Hops extract 0.05%, glycerin 0.51%, citric acid 0.13%, persimmon tannin 0.06%, lysozyme 0.06%, artemisia annua extract 2.0%, gentian root extract 3.0%, mugwort extract 3.0%, and the balance is deionized water.

[0049] The contents of alpha-acid and beta-acid in the hop extract are respectively in the range of 400-600 mg / g and 100-300 mg / g.

[0050] Example 5

[0051] A pet sterilizer and deodorant (can be used in the oral cavity), comprising the following raw materials in the following mass percentages:

[0052] Hops extract 0.13%, glycerin 1.49%, citric acid 0.44%, persimmon tannin 0.28%, lysozyme 0.25%, artemisia annua extract 3.4%, gentian root extract 3.5%, mugwort extract 3.5%, and the balance is deionized water.

[0053] The contents of alpha-acid and beta-acid in the hop extract are respectively in the range of 400-600 mg / g and 100-300 mg / g.

[0054] Example 6

[0055] A pet sterilizer and deodorant (can be used in the oral cavity), comprising the following raw materials in the following mass percentages:

[0056] Hops extract 0.2%, glycerin 2.97%, citric acid 1%, persimmon tannin 0.5%, lysozyme 0.5%, artemisia annua extract 5%, gentian root extract 4%, mugwort extract 4%, and the balance is deionized water.

[0057] The contents of alpha-acid and beta-acid in the hop extract are respectively in the range of 400-600 mg / g and 100-300 mg / g.

[0058] Example 7

[0059] A pet sterilizer and deodorizer (for external use only), comprising the following raw materials in the following mass percentages:

[0060] Hops extract 0.06%, glycerin 0.52%, citric acid 0.11%, persimmon tannin 0.05%, lysozyme 0.05%, antibacterial agent 1%, zinc ricinoleate 3%, and the balance is deionized water.

[0061] The contents of alpha-acid and beta-acid in the hop extract are respectively in the range of 400-600 mg / g and 100-300 mg / g.

[0062] The antibacterial agent is a modified quaternary ammonium salt antibacterial liquid, which is prepared by the following operations:

[0063] (1) Dissolve 10 g of chitosan in 1.8 L of 50% ethanol solution, and then add 10 mL of 30% glacial acetic acid solution dropwise to form mixture A;

[0064] (2) adding a glutaraldehyde solution having a volume concentration of 50% to mixture A and stirring at room temperature for 60 minutes to form mixture B, wherein the amount of the glutaraldehyde solution added in (2) is controlled so that the molar ratio of glutaraldehyde to -NH2 is 1:1.5;

[0065] (3) Sodium triacetoxyborohydride was added to dichloroethane to dissolve the mixture to prepare a mixture C, wherein the ratio of sodium triacetoxyborohydride to dichloroethane was 1 g:40 mL;

[0066] (4) Add mixture B to mixture C, control the mass ratio of chitosan added in mixture B described in (4) to sodium triacetoxyborohydride in mixture C to be 1:5, stir for 90 minutes, then add N-bromosuccinimide and continue stirring for 90 minutes to obtain a modified quaternary ammonium salt antibacterial liquid, wherein the mass ratio of N-bromosuccinimide to chitosan described in (1) is 0.8:1.

[0067] Example 8

[0068] A pet sterilizer and deodorizer (for external use only), comprising the following raw materials in the following mass percentages:

[0069] Hops extract 0.14%, glycerin 1.67%, citric acid 0.54%, persimmon tannin 0.26%, lysozyme 0.25%, antibacterial agent 2%, zinc ricinoleate 3.5%, and the balance is deionized water.

[0070] The contents of alpha-acid and beta-acid in the hop extract are respectively in the range of 400-600 mg / g and 100-300 mg / g.

[0071] The antibacterial agent is a modified quaternary ammonium salt antibacterial liquid, which is prepared by the following operations:

[0072] (1) Dissolve 10 g of chitosan in 1.9 L of 50% ethanol solution, and then add 15 mL of 30% glacial acetic acid solution dropwise to form mixture A;

[0073] (2) adding a glutaraldehyde solution having a volume concentration of 50% to mixture A and stirring at room temperature for 75 minutes to form mixture B, wherein the amount of the glutaraldehyde solution added in (2) is controlled so that the molar ratio of glutaraldehyde to -NH2 is 1:1.7;

[0074] (3) Sodium triacetoxyborohydride was added to dichloroethane to dissolve the mixture to prepare a mixture C, wherein the ratio of sodium triacetoxyborohydride to dichloroethane was 1 g:45 mL;

[0075] (4) Add mixture B to mixture C, control the mass ratio of chitosan added in mixture B described in (4) to sodium triacetoxyborohydride in mixture C to be 1:4, stir for 105 minutes, then add N-bromosuccinimide and continue stirring for 105 minutes to obtain a modified quaternary ammonium salt antibacterial liquid, wherein the mass ratio of N-bromosuccinimide to chitosan described in (1) is 1:1.

[0076] Example 9

[0077] A pet sterilizer and deodorizer (for external use only), comprising the following raw materials in the following mass percentages:

[0078] Hops extract 0.19%, glycerin 3%, citric acid 0.99%, persimmon tannin 0.5%, lysozyme 0.5%, antibacterial agent 3%, zinc ricinoleate 4%, the balance is deionized water.

[0079] The contents of alpha-acid and beta-acid in the hop extract are respectively in the range of 400-600 mg / g and 100-300 mg / g.

[0080] The antibacterial agent is a modified quaternary ammonium salt antibacterial liquid, which is prepared by the following operations:

[0081] (1) Dissolve 10 g of chitosan in 2 L of 50% ethanol solution, and then add 20 mL of 30% glacial acetic acid solution dropwise to form mixture A;

[0082] (2) adding a glutaraldehyde solution having a volume concentration of 50% to mixture A and stirring at room temperature for 90 minutes to form mixture B, wherein the amount of the glutaraldehyde solution added in (2) is controlled so that the molar ratio of glutaraldehyde to -NH2 is 1:2;

[0083] (3) Sodium triacetoxyborohydride was added to dichloroethane to dissolve the mixture to prepare a mixture C, wherein the ratio of sodium triacetoxyborohydride to dichloroethane was 1 g:50 mL;

[0084] (4) Add mixture B to mixture C, control the mass ratio of chitosan added in mixture B described in (4) to sodium triacetoxyborohydride in mixture C to be 1:3, stir for 120 minutes, then add N-bromosuccinimide and continue stirring for 120 minutes to obtain a modified quaternary ammonium salt antibacterial liquid, wherein the mass ratio of N-bromosuccinimide to chitosan described in (1) is 1.2:1.

[0085] Example 10

[0086] A pet sterilizer and deodorizer (for external use only), comprising the following raw materials in the following mass percentages:

[0087] Hops extract 0.06%, glycerin 0.52%, citric acid 0.11%, persimmon tannin 0.05%, lysozyme 0.05%, antibacterial agent 1%, zinc ricinoleate 3%, artemisia annua extract 2.0%, gentian root extract 3.0%, mugwort extract 3.0%, and the balance is deionized water.

[0088] The contents of alpha-acid and beta-acid in the hop extract are respectively in the range of 400-600 mg / g and 100-300 mg / g.

[0089] The antibacterial agent is a modified quaternary ammonium salt antibacterial liquid, which is prepared by the following operations:

[0090] (1) Dissolve 10 g of chitosan in 1.8 L of 50% ethanol solution, and then add 10 mL of 30% glacial acetic acid solution dropwise to form mixture A;

[0091] (2) adding a glutaraldehyde solution having a volume concentration of 50% to mixture A and stirring at room temperature for 60 minutes to form mixture B, wherein the amount of the glutaraldehyde solution added in (2) is controlled so that the molar ratio of glutaraldehyde to -NH2 is 1:1.5;

[0092] (3) Sodium triacetoxyborohydride was added to dichloroethane to dissolve the mixture to prepare a mixture C, wherein the ratio of sodium triacetoxyborohydride to dichloroethane was 1 g:40 mL;

[0093] (4) Add mixture B to mixture C, control the mass ratio of chitosan added in mixture B described in (4) to sodium triacetoxyborohydride in mixture C to be 1:5, stir for 90 minutes, then add N-bromosuccinimide and continue stirring for 90 minutes to obtain a modified quaternary ammonium salt antibacterial liquid, wherein the mass ratio of N-bromosuccinimide to chitosan described in (1) is 0.8:1.

[0094] Example 11

[0095] A pet sterilizer and deodorizer (for external use only), comprising the following raw materials in the following mass percentages:

[0096] Hops extract 0.14%, glycerin 1.67%, citric acid 0.54%, persimmon tannin 0.26%, lysozyme 0.25%, antibacterial agent 2%, zinc ricinoleate 3.5%, artemisia annua extract 3.4%, gentian root extract 3.5%, mugwort extract 3.5%, and the balance is deionized water.

[0097] The contents of alpha-acid and beta-acid in the hop extract are respectively in the range of 400-600 mg / g and 100-300 mg / g.

[0098] The antibacterial agent is a modified quaternary ammonium salt antibacterial liquid, which is prepared by the following operations:

[0099] (1) Dissolve 10 g of chitosan in 1.9 L of 50% ethanol solution, and then add 15 mL of 30% glacial acetic acid solution dropwise to form mixture A;

[0100] (2) adding a glutaraldehyde solution having a volume concentration of 50% to mixture A and stirring at room temperature for 75 minutes to form mixture B, wherein the amount of the glutaraldehyde solution added in (2) is controlled so that the molar ratio of glutaraldehyde to -NH2 is 1:1.7;

[0101] (3) Sodium triacetoxyborohydride was added to dichloroethane to dissolve the mixture to prepare a mixture C, wherein the ratio of sodium triacetoxyborohydride to dichloroethane was 1 g:45 mL;

[0102] (4) Add mixture B to mixture C, control the mass ratio of chitosan added in mixture B described in (4) to sodium triacetoxyborohydride in mixture C to be 1:4, stir for 105 minutes, then add N-bromosuccinimide and continue stirring for 105 minutes to obtain a modified quaternary ammonium salt antibacterial liquid, wherein the mass ratio of N-bromosuccinimide to chitosan described in (1) is 1:1.

[0103] Example 12

[0104] A pet sterilizer and deodorizer (for external use only), comprising the following raw materials in the following mass percentages:

[0105] Hops extract 0.19%, glycerin 3%, citric acid 0.99%, persimmon tannin 0.5%, lysozyme 0.5%, antibacterial agent 3%, zinc ricinoleate 4%, artemisia annua extract 5%, gentian root extract 4%, mugwort extract 4%, and the balance is deionized water.

[0106] The contents of alpha-acid and beta-acid in the hop extract are respectively in the range of 400-600 mg / g and 100-300 mg / g.

[0107] The antibacterial agent is a modified quaternary ammonium salt antibacterial liquid, which is prepared by the following operations:

[0108] (1) Dissolve 10 g of chitosan in 2 L of 50% ethanol solution, and then add 20 mL of 30% glacial acetic acid solution dropwise to form mixture A;

[0109] (2) adding a glutaraldehyde solution having a volume concentration of 50% to mixture A and stirring at room temperature for 90 minutes to form mixture B, wherein the amount of the glutaraldehyde solution added in (2) is controlled so that the molar ratio of glutaraldehyde to -NH2 is 1:2;

[0110] (3) Sodium triacetoxyborohydride was added to dichloroethane to dissolve the mixture to prepare a mixture C, wherein the ratio of sodium triacetoxyborohydride to dichloroethane was 1 g:50 mL;

[0111] (4) Add mixture B to mixture C, control the mass ratio of chitosan added in mixture B described in (4) to sodium triacetoxyborohydride in mixture C to be 1:3, stir for 120 minutes, then add N-bromosuccinimide and continue stirring for 120 minutes to obtain a modified quaternary ammonium salt antibacterial liquid, wherein the mass ratio of N-bromosuccinimide to chitosan described in (1) is 1.2:1.

[0112] Comparative Example 1

[0113] A pet sterilizer and deodorizer (can be used in the oral cavity) does not contain lysozyme and uses persimmon tannin instead, compared with Example 1, and includes the following raw materials in the following mass percentages:

[0114] Hops extract 0.06%, glycerin 0.52%, citric acid 0.11%, persimmon tannin 0.1%, and the balance is deionized water.

[0115] The contents of alpha-acid and beta-acid in the hop extract are respectively in the range of 400-600 mg / g and 100-300 mg / g.

[0116] Comparative Example 2

[0117] A pet sterilizer and deodorizer (can be used in the oral cavity) does not contain persimmon tannin, but uses lysozyme instead, compared with Example 2, and includes the following raw materials in the following mass percentages:

[0118] Hops extract 0.14%, glycerol 1.67%, citric acid 0.54%, lysozyme 0.51%, and the balance is deionized water.

[0119] The contents of alpha-acid and beta-acid in the hop extract are respectively in the range of 400-600 mg / g and 100-300 mg / g.

[0120] Comparative Example 3

[0121] A pet sterilizer and deodorant (can be used in the oral cavity), compared with Example 4, does not include Artemisia annua extract and uses Gentiana root extract instead, and includes the following raw materials in the following mass percentages:

[0122] Hops extract 0.05%, glycerin 0.51%, citric acid 0.13%, persimmon tannin 0.06%, lysozyme 0.06%, gentian root extract 5.0%, wormwood extract 3.0%, and the balance is deionized water.

[0123] The contents of alpha-acid and beta-acid in the hop extract are respectively in the range of 400-600 mg / g and 100-300 mg / g.

[0124] Comparative Example 4

[0125] A pet sterilizer and deodorant (can be used in the oral cavity), compared with Example 5, does not include gentian root extract and uses wormwood extract instead, and includes the following raw materials in the following mass percentages:

[0126] Hops extract 0.13%, glycerin 1.49%, citric acid 0.44%, persimmon tannin 0.28%, lysozyme 0.25%, artemisia annua extract 3.4%, mugwort extract 7%, and the balance is deionized water.

[0127] The contents of alpha-acid and beta-acid in the hop extract are respectively in the range of 400-600 mg / g and 100-300 mg / g.

[0128] Comparative Example 5

[0129] A pet sterilizer and deodorant (can be used in the oral cavity), compared with Example 6, does not include wormwood extract and uses Artemisia annua extract instead, and includes the following raw materials in the following mass percentages:

[0130] Hops extract 0.2%, glycerin 2.97%, citric acid 1%, persimmon tannin 0.5%, lysozyme 0.5%, artemisia annua extract 9%, gentian root extract 4%, and the balance is deionized water.

[0131] The contents of alpha-acid and beta-acid in the hop extract are respectively in the range of 400-600 mg / g and 100-300 mg / g.

[0132] Comparative Example 6

[0133] A pet sterilizer and deodorizer (for external use only) is prepared, compared with Example 7, except that zinc ricinoleate is not included and an antibacterial agent is used instead. The pet sterilizer and deodorizer include the following raw materials in the following mass percentages:

[0134] Hops extract 0.06%, glycerin 0.52%, citric acid 0.11%, persimmon tannin 0.05%, lysozyme 0.05%, antibacterial agent 4%, and the balance is deionized water.

[0135] The contents of alpha-acid and beta-acid in the hop extract are respectively in the range of 400-600 mg / g and 100-300 mg / g.

[0136] Comparative Example 7

[0137] A pet sterilizing and deodorizing agent (for external use only) does not include an antibacterial agent compared to Example 7, but uses zinc ricinoleate instead. The agent includes the following raw materials in the following mass percentages:

[0138] Hops extract 0.06%, glycerol 0.52%, citric acid 0.11%, persimmon tannin 0.05%, lysozyme 0.05%, zinc ricinoleate 4%, and the balance is deionized water.

[0139] The contents of alpha-acid and beta-acid in the hop extract are respectively in the range of 400-600 mg / g and 100-300 mg / g.

[0140] Comparative Example 8

[0141] A pet sterilizer and deodorizer (for external use only) is prepared in accordance with Example 8, except that the modified quaternary ammonium salt antibacterial liquid is replaced with a quaternary ammonium salt antibacterial liquid. The pet sterilizer and deodorizer comprises the following raw materials in the following weight percentages:

[0142] Hops extract 0.14%, glycerin 1.67%, citric acid 0.54%, persimmon tannin 0.26%, lysozyme 0.25%, antibacterial agent 2%, zinc ricinoleate 3.5%, and the balance is deionized water.

[0143] The contents of alpha-acid and beta-acid in the hop extract are respectively in the range of 400-600 mg / g and 100-300 mg / g.

[0144] The pet sterilizing and deodorizing agents prepared in Examples 1-12 and Comparative Examples 1-8 were subjected to the following tests:

[0145] Test Example 1 Deodorization Test

[0146] Tested according to CJ / T 516-2017 6.19:

[0147] Under normal temperature and pressure conditions, 15L of pollutant gas was passed through a large bubble absorption tube containing 10mL of sample (diluted 20 times) at a flow rate of 1L / min for 24 hours. The treated gas was collected and the concentration was analyzed. The deodorization efficiency of ammonia, trimethylamine and hydrogen sulfide was calculated respectively. The results are shown in Table 1.

[0148] Table 1

[0149]

[0150] As shown in Table 1, the pet sterilizing and deodorizing agents (for oral use) provided in Examples 1-3 of the present invention exhibit significantly improved deodorizing efficiency compared to Comparative Examples 1 and 2, demonstrating excellent deodorizing performance. Furthermore, the combination of Artemisia annua extract, Gentiana root extract, and Artemisia argyi extract with Examples 1-3 significantly improved deodorizing efficiency compared to Comparative Examples 3-5, synergistically. Furthermore, the combination of an antibacterial agent and zinc ricinoleate with hops extract, the antibacterial agent, and zinc ricinoleate significantly improved deodorizing efficiency compared to Comparative Examples 6 and 7, which did not undergo the three-combination treatment. Furthermore, Examples 10-12 exhibited excellent deodorizing efficiency in the presence of all the combined ingredients.

[0151] Test Example 2 Sterilization Test

[0152] The on-site sterilization and disinfection test was conducted using a cat breeding factory as an experiment, including the following operations:

[0153] (1) Randomly select the wall surface and mark three pieces with an area of ​​25cm each using standard plates. 2 The blocks are used for sampling before disinfection, sampling after chemical disinfection and sampling for deodorizing spray disinfection.

[0154] (2) Spray purified water on the sampling area, then wet a sterile cotton swab in a test tube containing 5ml diluent, smear the sample on the sampling area, and move it back and forth 8 times horizontally and vertically. After sampling, cut the sampling end of the cotton swab into the original diluent test tube in a sterile manner, shake it for 20 seconds, and make appropriate dilution to serve as the negative control group sample.

[0155] (3) Dilute the chemical disinfectant according to the instructions and spray it on the sampling area for disinfection. Then wet a sterile cotton swab in a test tube containing 5 mL of diluent and apply it to one area for sampling, moving it back and forth 8 times horizontally and vertically. After sampling, cut the sampling end of the cotton swab into the original diluent test tube in a sterile manner, shake it for 20 seconds, and make appropriate dilution to serve as the positive control group sample.

[0156] (4) Spray the deodorant spray directly onto the sampling area for deodorization and disinfection. Then, wet a sterile cotton swab in a test tube containing 5 mL of diluent and apply the sample to the deodorization and disinfection area, moving back and forth 8 times horizontally and vertically. After sampling, cut the sampling end of the cotton swab into the original dilution test tube in a sterile manner and shake it with an electric mixer for 20 seconds. This is the sample of the deodorization and disinfection group.

[0157] (5) Take 1.0 mL of each sample from the positive control group, negative control group, and deodorization and disinfection group, inoculate the plates using the agar pouring method, inoculate two plates for each sample, and incubate them in a 37°C incubator for 48 hours to observe the final results.

[0158] (6) Calculate the kill logarithm

[0159] The test was repeated three times, and the number of colonies (cfu) in each group was calculated and converted into a logarithmic value (N). The killing logarithm was then calculated as follows:

[0160] Killing logarithm (KL) = logarithm of the average viable bacteria concentration in the control group (No) - logarithm of the viable bacteria concentration in the experimental group (Nx)

[0161] The test was repeated three times. The positive control group should have more bacteria growing, and the negative control group should have no bacteria growing. The average killing logarithm of the disinfected samples was ≥1, which was considered qualified for disinfection. The results are shown in Table 2.

[0162] Table 2

[0163]

[0164] As shown in Table 2, among Examples 1-12 of the present invention and Comparative Examples 1-8, except for the bactericidal effect of the bactericidal deodorant obtained in Comparative Example 1 which did not meet the standard, the others all had antibacterial effects.

[0165] Throughout the specification, references to terms such as "one embodiment," "example," or "specific example" indicate that the specific features, structures, materials, or characteristics described in conjunction with that embodiment or example are included in at least one embodiment or example of the present invention. In this specification, schematic representations of these terms do not necessarily refer to the same embodiment or example. Furthermore, the specific features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples.

[0166] The above contents are merely examples and explanations of the present invention. Those skilled in the art may make various modifications or additions to the described specific embodiments or replace them in similar ways. As long as they do not deviate from the invention or exceed the scope defined by the claims, they should all fall within the scope of protection of the present invention.

Claims

1. A pet sterilizing and deodorizing agent, characterized in that: The bactericidal deodorant comprises the following raw materials in percentage by mass: Hops extract 0.05-0.2%, stabilizer 0.5-3%, citric acid 0.1-1%, persimmon tannin 0.05%-0.5%, lysozyme 0.05%-0.5%, antibacterial agent 1-3%, zinc ricinoleate 3-4%, artemisia annua extract 2-5%, gentian root extract 3-4% and mugwort extract 3-4%, and the balance is deionized water.

2. The pet sterilizing and deodorizing agent according to claim 1, characterized in that: The contents of alpha-acid and beta-acid in the hop extract are respectively in the range of 400-600 mg / g and 100-300 mg / g.

3. The pet sterilizing and deodorizing agent according to claim 1, characterized in that: The stabilizer is at least one of acetate buffer and glycerol.

4. The pet sterilizing and deodorizing agent according to claim 1, characterized in that: The antibacterial agent is a modified quaternary ammonium salt antibacterial liquid, which is prepared by the following operations: (1) Chitosan was dissolved in an ethanol solution with a volume concentration of 50%, and then a glacial acetic acid solution with a mass concentration of 30% was added dropwise to form a mixture A; (2) Add 50% glutaraldehyde solution to mixture A and stir at room temperature for 60-90 minutes to form mixture B; (3) adding sodium triacetoxyborohydride into dichloroethane to dissolve the mixture to prepare a mixture C; (4) Add mixture B to mixture C, stir for 90-120 min, then add N-bromosuccinimide and continue stirring for 90-120 min to obtain a modified quaternary ammonium salt antibacterial liquid.

5. The pet sterilizing and deodorizing agent according to claim 4, characterized in that: The amount ratio of chitosan, ethanol solution and glacial acetic acid solution described in (1) is 1g:180-200mL:1-2mL.

6. The pet sterilizing and deodorizing agent according to claim 4, characterized in that: The ratio of sodium triacetoxyborohydride to dichloroethane in (3) is 1 g:40-50 mL.

7. The pet sterilizing and deodorizing agent according to claim 4, characterized in that: The mass ratio of the amount of chitosan added in the mixture B described in (4) to the amount of sodium triacetoxyborohydride in the mixture C is controlled to be 1:3-5, and the mass ratio of the N-bromosuccinimide to the chitosan described in (1) is controlled to be 0.8-1.2:1.

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