Plasmid mixing apparatus

Abstract

The application discloses a kind of plasmid mixing equipment, including test tube rack, material moving assembly, multiple groups of feeding mechanism and pipetting assembly;The material moving assembly includes displacement driver that can move test tube to feeding mechanism and code scanning assembly, the feeding mechanism includes movable positioning carrier, cover opening assembly and cover closing assembly, the cover opening assembly includes turnover support plate, L-shaped fastener is provided on the turnover support plate, when the turnover support plate is parallel with positioning carrier, the tube cover opening end of test tube on the positioning carrier can be clamped into the L-shaped fastener, by turning over the turnover support plate to open the tube cover, multiple groups of the cover closing assembly correspond to the position of positioning carrier, the pipetting assembly includes pipetting driver and pipette gun that adds reagent in test tube to empty tube.The application automatically adds accurate dose of multiple mixed reagents, improves the efficiency of reagent plasmid mixing, and ensures the accuracy of plasmid mixing.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of reagent mixing, in particular to a plasmid mixing device. BACKGROUND

[0002] A test tube is a small container made of glass or transparent plastic tube commonly used in chemical laboratories and life science laboratories, and a test tube used for biological cell or microbial culture is also called a culture tube. The test tube usually includes a tube body, the lower end of the tube body is closed as a tube bottom, and the upper end of the tube body is provided with a tube opening. The tube opening has a flat opening form and a rolled opening form, that is, the opening of the tube opening slightly protrudes outward to form a flange with an outer diameter larger than the outer diameter of other parts of the tube body, facilitating clamping of the tube body and locking of the tube cover, and preventing falling off; the tube bottom wall can have different types such as flat bottom, round bottom and conical bottom; the tube body is usually cylindrical in structure, and the tube body and the tube cover are connected and buckled.

[0003] According to different plasmid mixing formulas, different types of reagents need to be added to the test tube, and manual addition cannot accurately add the amount of each type of reagent, and cannot guarantee that the mixed reagents taken are the correct type of reagent, which leads to errors in reagent mixing in the test tube, reduces the efficiency of reagent plasmid mixing, and also causes cost loss to the enterprise.

[0004] Therefore, it is necessary to design a plasmid mixing device to solve the above problems. SUMMARY

[0005] In order to overcome the above-mentioned defects, the purpose of the present application is to provide a plasmid mixing device which automatically adds accurate doses of multiple mixed reagents, improves the efficiency of reagent plasmid mixing, and guarantees the accuracy of plasmid mixing.

[0006] In order to achieve the above purpose, the technical scheme adopted by the present application is: a plasmid mixing device, comprising a test tube placing rack, a material moving assembly, a plurality of feeding mechanisms and a pipetting assembly; the material moving assembly comprises a displacement driving part capable of moving the test tube to the feeding mechanism and a code scanning assembly, the feeding mechanism comprises a movable positioning carrier, an opening cover assembly and a closing assembly, the opening cover assembly comprises a turnover support plate, the turnover support plate is provided with an L-shaped buckling part, when the turnover support plate is parallel to the positioning carrier, the open end of the tube cover of the test tube on the positioning carrier can be clamped into the L-shaped buckling part, the tube cover is opened by turning over the turnover support plate, a plurality of closing assemblies correspond to the positions of the positioning carrier, and the pipetting assembly comprises a pipetting driving part and a pipetting gun for adding reagents in the test tube to an empty tube.

[0007] Further, the positioning carrier is slidably connected with the fixed support on the feeding driving element through a moving rail, the positioning carrier comprises a T-shaped support and a plurality of vertically arranged test tube placing grooves, the moving plate at the bottom of the T-shaped support penetrates through the fixed support, and horizontal insertion grooves for the insertion of clamping assemblies are arranged at positions corresponding to the test tube placing grooves on the two sides of the T-shaped support.

[0008] Further, the clamping assembly comprises a connecting column penetrating through the fixed support, one end of the connecting column close to the T-shaped support is provided with a clamping block, and the portion of the connecting column between the clamping block and the fixed support is covered with a spring.

[0009] Further, the bottom of the moving plate is elastically connected with the bottom surface of the fixed support through a rectangular spring, when the moving plate moves towards the displacement driving element, the caps of the test tubes are away from or inserted into the L-shaped buckling members, the lower end surface of the moving plate is provided with a connecting block, the surface of the fixed support is provided with a fixed supporting block, and the two ends of the rectangular spring are connected with the connecting block and the fixed supporting block respectively. When the moving plate moves towards the displacement driving element, the rectangular spring is in a compressed state, the caps of the test tubes are not in contact with the L-shaped buckling members, the test tubes will not interfere with the rotation of the overturning support plate during the rotation of the overturning support plate, and when no additional pressure is applied to the rectangular spring, the elastic force of the rectangular spring can drive the moving plate to reset and move away from the displacement driving element, at this time, the caps of the test tubes on the positioning carrier are clamped into the L-shaped buckling members, and the overturning driving element drives the overturning support plate to overturn again, so that the automatic uncapping of a plurality of test tubes can be realized.

[0010] Further, the uncapping assembly further comprises an overturning driving element for driving the rotation of the overturning support plate and a cam support arranged on the side of the feeding driving element and away from the pipetting assembly, the cam support is provided with a cam follower, and when the positioning carrier is in contact with the cam follower, the cam follower can push the moving plate to move towards the displacement driving element. At this time, the spring is not under pressure and is in a relaxed state, there is space for the insertion of the test tubes between the clamping block and the test tube placing groove, so as to facilitate the feeding of the test tubes, when the cam follower is separated from the moving plate, the rectangular spring drives the moving plate to reset, the spring is in a compressed state, the clamping block abuts against the test tubes and clamps the test tubes in the test tube placing groove, so as to effectively avoid the movement of the test tubes. The cam follower can realize the loosening and clamping of the test tubes in the test tube placing groove.

[0011] Further, the cover assembly comprises a plurality of pressing columns penetrating through the turnover support plate and a spring II covering the pressing columns, a cover pressing block is arranged on one end of the pressing column close to the L-shaped fastening piece, and the two ends of the spring II are connected to the surface of the turnover support plate and the end of the pressing column away from the L-shaped fastening piece, respectively. The turnover driving piece drives the turnover support plate to turn over again, and the cap on the reagent is automatically covered through the cover pressing block thereon, so that the staff does not need to manually open the cap, and the working efficiency of reagent plasmid mixing is effectively improved.

[0012] Further, the needle feeding assembly and the needle discharging assembly are further included, the needle feeding assembly comprises a needle moving module and a needle placing carrier located thereon, and the needle feeding assembly is located corresponding to the position of the pipetting assembly.

[0013] Further, the needle discharging assembly comprises a discharging support, a discharging port is arranged at the top of the discharging support, and an inclined discharging piece is connected to the bottom of the discharging support.

[0014] Further, the material moving assembly further comprises a moving electric clamp jaw, and the code scanning assembly is specifically a code scanning camera. The code scanning camera can scan all the test tubes on the test tube placing rack one by one, mark the positions of each test tube and the types of reagents in the test tubes, and record in the external display screen, so that the accuracy of test tube grabbing in subsequent reagent mixing is ensured.

[0015] The displacement driving piece comprises a moving frame supporting the moving electric clamp jaw and the code scanning camera and a driving motor, the driving motor drives a driving wheel to rotate, the driving wheel is connected with a driven wheel through a transmission belt, so that the synchronous wheel coaxially arranged with the driven wheel is driven to rotate, so that the moving frame moves in the front and back directions, and displacement driving pieces for driving the code scanning assembly and the moving electric clamp jaw to move are also arranged on the moving frame. The displacement driving piece is not limited to the above structure, and can also be a linear module or an electric screw rod, as long as the code scanning assembly and the moving electric clamp jaw can move in the horizontal direction.

[0016] The device support for supporting the material moving assembly and the whole machine protective cover are further included.

[0017] Advantages of the present application:

[0018] 1. In the present application, the material moving assembly, the feeding mechanism and the pipetting assembly cooperate with each other, the L-shaped fastening piece on the turnover support plate is turned over to realize automatic uncapping of a plurality of test tubes, and the efficiency of subsequent plasmid mixing is effectively improved; the pipetting assembly can automatically replace the pipetting needle and automatically add an accurate dose of a plurality of mixed reagents into the empty test tube, so that the accuracy of plasmid mixing is ensured.

[0019] 2、The novel setting clamping assembly, when opening the cover of test tube, the clamping block can clamp the test tube in the test tube placing groove, avoid the process of opening the cover to move up and down the test tube, guarantee the efficiency of test tube automatic opening cover. BRIEF DESCRIPTION OF DRAWINGS

[0020] Figure 1 The overall structure of an embodiment of the present application is shown in the axial view.

[0021] Figure 2 The partial structure of an embodiment of the present application is shown in the axial view.

[0022] Figure 3 Another perspective view of the partial structure of an embodiment of the present application is shown in the axial view.

[0023] Figure 4 The overall structure of the feeding mechanism of an embodiment of the present application is shown in the axial view.

[0024] Figure 5 The partial structure of the feeding mechanism of an embodiment of the present application is shown in the axial view.

[0025] Figure 6 Another partial structure of the feeding mechanism of an embodiment of the present application is shown in the axial view.

[0026] Figure 7 Still another partial structure of the feeding mechanism of an embodiment of the present application is shown in the axial view.

[0027] Figure 8 The structure of the pipetting assembly of an embodiment of the present application is shown in the axial view.

[0028] As shown in the figure, 1 is a whole machine protective cover, 2 is a device support, 3 is a test tube rack, 4 is a material moving assembly, 41 is a displacement driving part, 411 is a driving motor, 412 is a driving wheel, 413 is a driven wheel, 414 is a transmission belt, 415 is a moving frame, 42 is a code scanning assembly, 43 is a moving electric clamp jaw, 5 is a feeding mechanism, 51 is a cover opening assembly, 511 is a cam follower, 512 is a cam support, 513 is a turnover driving part, 514 is a turnover support plate, 515 is an L-shaped buckling part, 52 is a feeding driving part, 53 is a fixed support, 54 is a positioning carrier, 541 is a T-shaped support, 542 is a test tube placing groove, 543 is a limiting block, 544 is a horizontal slot, 545 is a moving plate, 546 is an inclined surface, 55 is a clamping assembly, 551 is a clamping block, 552 is a spring I, 553 is a connecting column, 56 is a cover closing assembly, 561 is a cover closing block, 562 is a pressing column, 563 is a spring II, 57 is a moving slide rail, 58 is a fixed support block, 59 is a connecting block, 60 is a rectangular spring, 7 is a pipetting assembly, 71 is a pipetting driving part, 72 is a pipetting gun, 74 is a liquid storage support, 8 is a needle head feeding assembly, 81 is a needle head placing carrier, 82 is a needle head moving module, 9 is a needle head discharging assembly, 91 is a discharging support, 92 is a discharging port, and 93 is an inclined discharging part. DETAILED DESCRIPTION

[0029] The advantages and features of the present application can be more easily understood by those skilled in the art from the preferred embodiments of the present application described in detail below in conjunction with the accompanying drawings, so that the scope of protection of the present application can be more clearly defined.

[0030] Referring to the accompanying drawings Figures 1 to 8 As shown in the figure, a plasmid mixing device in the embodiment includes a test tube rack 3, a material moving assembly 4, multiple sets of feeding mechanisms 5, and a pipetting assembly 7. The material moving assembly 4 includes a displacement driving part 41 and a code scanning assembly 42, which can move the test tube to the feeding mechanism 5. The feeding mechanism 5 includes a movable positioning carrier 54, a cover opening assembly 51, and a cover closing assembly 56. The cover opening assembly 51 includes a turnover support plate 514, and the turnover support plate 514 is provided with an L-shaped buckling part 515. When the turnover support plate 514 is parallel to the positioning carrier 54, the open end of the cover of the test tube on the positioning carrier 54 can be clamped into the L-shaped buckling part 515, and the cover is opened by turning over the turnover support plate 514. Multiple sets of the cover closing assembly 56 correspond to the positions of the positioning carrier 54. The pipetting assembly 7 includes a pipetting driving part 71 and a pipetting gun 72, which adds the reagent in the test tube to an empty tube.

[0031] The positioning carrier 54 is slidably connected with the fixed support 53 on the feeding driving element 52 through the moving slide rail 57, and the positioning carrier 54 comprises a T-shaped support 541 and a plurality of vertically arranged test tube placing grooves 542, the moving plate 545 at the bottom of the T-shaped support 541 penetrates through the fixed support 53, and horizontal insertion grooves 544 for inserting the clamping assembly 55 are arranged at the positions corresponding to the test tube placing grooves 542 on the two sides of the T-shaped support 541.

[0032] The clamping assembly 55 comprises a connecting column 553 penetrating through the fixed support 53, and a clamping block 551 is arranged at one end of the connecting column 553 close to the T-shaped support 541, and the part of the connecting column 553 between the clamping block 551 and the fixed support 53 is covered with a spring 552.

[0033] The bottom of the moving plate 545 is elastically connected with the bottom surface of the fixed support 53 through the rectangular spring 60, and when the moving plate 545 moves towards the direction close to or away from the displacement driving element 41, the cover of the test tube is away from or inserted into the L-shaped buckling part 515. A connecting block 59 is arranged on the lower end surface of the moving plate 545, a fixed supporting block 58 is arranged on the surface of the fixed support 53, and the two ends of the rectangular spring 60 are connected with the connecting block 59 and the fixed supporting block 58 respectively. When the moving plate 545 moves close to the displacement driving element 41, the rectangular spring 60 is in a compressed state, and the cover of the test tube is not in contact with the L-shaped buckling part 515, so that the test tube does not interfere with the rotation of the turnover supporting plate 514 during the rotation process, and when no additional pressure is applied on the rectangular spring 60, the elastic force of the rectangular spring 60 can drive the moving plate 545 to reset and move away from the displacement driving element 41, and at this time, the open end of the cover of the test tube on the positioning carrier 54 is clamped into the L-shaped buckling part 515, and the turnover driving element 513 drives the turnover supporting plate 514 to rotate again, so that the automatic uncapping of a plurality of test tubes can be realized.

[0034] The feeding mechanism 5 further comprises a feeding driving element 52, and an inclined surface 546 is arranged on one corner of the moving plate 545 away from the pipetting assembly 7, so that the moving plate 545 is gradually separated from the cam follower 511. The feeding driving element 52 is a driving cylinder or a linear module, as long as it can drive the positioning carrier 54 to move towards the direction of the pipetting assembly 7.

[0035] The cover opening assembly 51 further comprises a turnover driving member 513 for driving the turnover supporting plate 514 to rotate, and a cam bracket 512 arranged at the side of the feeding driving member 52 and away from the pipetting assembly 7, wherein the cam bracket 512 is provided with a cam follower 511, and when the positioning carrier 54 contacts with the cam follower 511, the cam follower 511 can push the moving plate 545 to move towards the displacement driving member 41. At this time, the spring 552 is not under pressure and is in a relaxed state, and the space between the clamping block 551 and the test tube placing groove 542 is provided for inserting the test tube, so as to facilitate the test tube feeding. When the cam follower 511 is separated from the moving plate 545, the rectangular spring 60 drives the moving plate 545 to reset, the spring 552 is in a compressed state, the clamping block 551 abuts against the test tube and clamps the test tube in the test tube placing groove 542, so as to effectively avoid the position movement of the test tube. The cam follower 511 is used to loosen and clamp the test tube in the test tube placing groove 542.

[0036] The cover closing assembly 56 comprises a plurality of pressing columns 562 penetrating through the turnover supporting plate 514 and a spring 563 covering the pressing columns 562, wherein one end of the pressing column 562 close to the L-shaped buckling member 515 is provided with a closing block 561, and the two ends of the spring 563 are connected to the surface of the turnover supporting plate 514 and the end of the pressing column 562 away from the L-shaped buckling member 515, respectively. The turnover driving member 513 drives the turnover supporting plate 514 to rotate again, and the closing block 561 on the turnover supporting plate 514 automatically closes the cap on the reagent, so that the staff does not need to manually open the cap, and the working efficiency of the reagent plasmid mixing is effectively improved.

[0037] The needle feeding assembly 8 and the needle discharging assembly 9 are further included, the needle feeding assembly 8 comprises a needle moving module 82 and a needle placing carrier 81 arranged thereon, and the needle feeding assembly 8 is located corresponding to the pipetting assembly 7. The pipetting assembly 7 further comprises a liquid storage bracket 74 arranged below the pipetting gun 72, so as to effectively avoid that the reagent solution remaining on the pipetting needle is dripped anywhere to affect the environment of the reagent plasmid mixing.

[0038] The needle discharging assembly 9 comprises a discharging bracket 91, the top of the discharging bracket 91 is provided with a discharging port 92, and the bottom of the discharging bracket 91 is connected with an inclined discharging member 93.

[0039] The material moving assembly 4 further comprises a moving electric clamp jaw 43, and the code scanning assembly 42 is specifically a code scanning camera. The code scanning camera can scan all the test tubes on the test tube placing rack 3 one by one, mark the position of each test tube and the type of reagent in the test tube, and record in the external display screen, so as to ensure the accuracy of the test tube grabbing in the subsequent reagent mixing.

[0040] The displacement driving part 41 comprises a moving electric clamp jaw 43 and a moving frame 415 supporting a code scanning camera, and a driving motor 411 driving a driving wheel 412 to rotate, the driving wheel 412 being connected with a driven wheel 413 through a transmission belt 414, thereby driving a synchronous wheel coaxially arranged with the driven wheel 413 to rotate, so as to realize the movement of the moving frame 415 in the front and back directions, the moving frame 415 being also provided with a displacement driving part 41 driving the code scanning assembly 42 and the moving electric clamp jaw 43 to move. The displacement driving part 41 is not limited to the above structure, and can also be a linear module or an electric screw rod, as long as it can realize the movement of the code scanning assembly 42 and the moving electric clamp jaw 43 in the horizontal direction

[0041] The device support 2 for supporting the material moving assembly 4 and the whole machine protective cover 1 are further included.

[0042] Working principle: The four-plate stock solution and two-plate empty test tubes are placed on the test tube placing frame 3, the displacement driving part 41 drives the code scanning assembly 42 thereon to scan all the test tubes on the test tube placing frame 3 one by one, mark the position of each test tube and the reagent type in the test tube, and record in the external display screen.

[0043] When the reagent plasmid mixing is performed, the moving electric clamp jaw 43 accurately grabs the test tube of the reagent to be mixed according to different formula requirements, and places it on the positioning carrier 54, at least one empty test tube is arranged on each group of the positioning carrier 54, then the positioning carrier 54 is moved to the liquid moving assembly 7 by the feeding linear module to add the reagent, wherein the two groups of the feeding mechanism 5 can realize the cyclic feeding of the reagent, effectively improving the efficiency of the reagent plasmid mixing.

[0044] In the initial state, the feeding linear module is at the cam follower 511, the cam follower 511 exerts pressure on the moving plate 545, the moving plate 545 moves along the moving slide rail 57 in the direction away from the cam follower 511, at this time, the rectangular spring 60 is in the compressed state, the spring 552 is in the relaxed state, and the space between the clamping block 551 and the test tube placing groove 542 can directly place the test tube, after the test tube is placed, the turnover driving part 513 drives the turnover support plate 514 to turn over, so that the surface thereof is parallel to the upper surface of the tube cover. Then the feeding linear module drives the positioning carrier 54 to move, so that the cam follower 511 and the moving plate 545 are separated, and the pressure is no longer exerted thereon. The elastic force of the rectangular spring 60 can drive the moving plate 545 to reset, at this time, the tube cover opening side of the test tube located on the positioning carrier 54 is clamped into the L-shaped buckling part 515, the turnover driving part 513 drives the turnover support plate 514 to turn over again, so that the automatic opening of multiple test tubes can be realized. In the process of opening the cover, the clamping block 551 clamps the test tube between itself and the test tube placing groove 542 under the elastic force of the spring 552, so that the test tube is prevented from moving up and down in the process of opening the cover.

[0045] After the automatic opening of the cover is completed, the pipetting driving part 71 drives the pipetting gun 72 to clamp the pipetting needle on the needle placing carrier 81 and to suck the stock solution in the test tube into the empty test tube to mix the plasmid, wherein the pipetting gun 72 automatically changes different pipetting needles when different stock solutions are sucked, and the used pipetting needles are uniformly collected through the discharge port 92. After the reagent plasmid in the test tube is mixed, the turnover driving part 513 drives the turnover support plate 514 to turn over again, and the cover pressing block 561 on the turnover support plate 514 automatically covers the tube cover on the reagent, so that the staff does not need to manually open the tube cover, and the working efficiency of the reagent plasmid mixing is effectively improved.

[0046] The above embodiments only serve to illustrate the technical concept and characteristics of the present application, the purpose is to enable the person skilled in the art to understand the content of the present application and implement it, and cannot limit the protection scope of the present application, any equivalent changes or modifications made according to the spirit and essence of the present application should be covered in the protection scope of the present application.

Claims

1. A plasmid mixing apparatus, characterized by: The application relates to a test tube feeding device, which comprises a test tube placing rack, a material moving assembly, a plurality of feeding mechanisms and a pipetting assembly; the material moving assembly comprises a displacement driving element and a code scanning assembly which can move the test tube to the feeding mechanism; the feeding mechanism comprises a movable positioning carrier, a cover opening assembly and a cover closing assembly; the cover opening assembly comprises a turnover support plate which is provided with an L-shaped buckling element; when the turnover support plate is parallel to the positioning carrier, the open end of the cover of the test tube on the positioning carrier can be clamped into the L-shaped buckling element; the cover is opened by turning over the turnover support plate; a plurality of cover closing assemblies are arranged in positions corresponding to the positioning carrier; the pipetting assembly comprises a pipetting driving element and a pipetting gun which can add reagent in the test tube to an empty tube; The positioning carrier is slidably connected with a fixed support on the feeding driving element through a moving slide rail; the positioning carrier comprises a T-shaped support and a plurality of vertically arranged test tube placing grooves; a moving plate at the bottom of the T-shaped support penetrates through the fixed support; horizontal insertion grooves for inserting clamping assemblies are arranged on the T-shaped support in positions corresponding to the test tube placing grooves; The clamping assembly comprises a connecting column which penetrates through the fixed support; one end of the connecting column close to the T-shaped support is provided with a clamping block; the part of the connecting column between the clamping block and the fixed support is covered with a spring; The bottom of the moving plate is elastically connected with the bottom surface of the fixed support through a rectangular spring; when the moving plate moves towards the displacement driving element or away from the displacement driving element, the cover of the test tube moves away from or into the L-shaped buckling element; The cover opening assembly further comprises a turnover driving element which drives the turnover support plate to rotate and a cam support which is arranged on the side of the feeding driving element and away from the pipetting assembly; the cam support is provided with a cam follower; when the positioning carrier contacts the cam follower, the cam follower can push the moving plate to move towards the displacement driving element.

2. The plasmid mixing apparatus of claim 1, wherein: The cover closing assembly comprises a plurality of pressing columns which penetrate through the turnover support plate and springs II which cover the pressing columns; one end of the pressing column close to the L-shaped buckling element is provided with a cover pressing block; the two ends of the spring II are respectively connected with the surface of the turnover support plate and one end of the pressing column away from the L-shaped buckling element.

3. The plasmid mixing device of claim 1, wherein: The application further comprises a needle feeding assembly and a needle discharging assembly; the needle feeding assembly comprises a needle moving module and a needle placing carrier thereon; the needle feeding assembly is arranged in a position corresponding to the pipetting assembly.

4. The plasmid mixing device of claim 3, wherein: The needle discharging assembly comprises a discharging support; the top of the discharging support is provided with a discharging port; the bottom of the discharging support is connected with an inclined discharging element.

5. The plasmid mixing device of claim 1, wherein: The material moving assembly further comprises a moving electric clamp jaw; the code scanning assembly is specifically a code scanning camera.

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