A fermentation method for producing yeast culture using camphor leaves and vinasse

Abstract

The present invention discloses a fermentation method for producing yeast culture using camphor leaf and distiller's grains, comprising the following steps: S1, culturing each bacterial species in a composite bacterial formula separately; S2, inoculating each bacterial species cultured in S1 into a camphor leaf and distiller's grains culture medium for anaerobic fermentation. The method produces a large number of bacterial protein cells with an overall protein content of approximately 17%, which can effectively replace some corn and soybean meal.

Description

Technical Field

[0001] The present invention relates to the field of biological agriculture, and in particular to a fermentation method for producing yeast culture by using camphor tree leaves and distiller's grains, a fermentation method, a fermentation product and applications thereof. Background Art

[0002] The current large-scale promotion and application of yeast culture production on the market are limited by two reasons: first, the commonly used strains in the process are relatively single, mainly Saccharomyces cerevisiae and Candida utilis; second, when used after fermentation, they are mainly in a moist state, and the use of high-temperature drying destroys a large amount of volatile low-molecular-weight effective active components produced during the fermentation process, resulting in a significant reduction in functional active nutrients.

[0003] The cost of fermentation raw materials for existing feed is high, the feed quality is poor, the amount of corn and soybean meal used is large, and the problem of competition for food between humans and animals is serious.

[0004] The above background technology is for facilitating understanding of the present invention and is not a known technology disclosed to the general public before the application of the present invention. Summary of the Invention

[0005] In view of the above-mentioned defects, on the one hand, the present invention provides a fermentation method of a camphor leaf-sorghum lees yeast culture, wherein the fermentation method adopts the present invention's Saccharomyces cerevisiae ACCC 41823, Candida utilis tropicalis ACCC 42866, Lindnerella fabienne ACCC 43892, Pichia pastoris CICC 56783, Bacillus subtilis CICC 76836, Lactobacillus paracasei CICC 38763, Lactobacillus casei ACCC 32876 and Clostridium butyricum CICC 61836, etc. for fermentation to obtain a large number of bacterial protein cells.

[0006] A fermentation method for producing yeast culture using camphor tree leaves and distiller's grains comprises the following steps:

[0007] S1, culturing each bacterial species in the composite bacterial formula separately;

[0008] S2: Take each strain cultured in S1 and inoculate it into the Cinnamomum camphora leaf-vintner's grains culture medium for anaerobic fermentation.

[0009] Furthermore, the composite bacteria formula comprises Saccharomyces cerevisiae ACCC 41823, Candida utilis tropicalis ACCC42866, Lindnerella fabienne ACCC 43892, Pichia pastoris CICC 56783, Bacillus subtilis CICC 76836, Lactobacillus paracasei CICC 38763, Lactobacillus casei ACCC 32876 and Clostridium butyricum CICC 61836.

[0010] Furthermore, the inoculation ratio of Saccharomyces cerevisiae ACCC 41823, Candida utilis tropicalis ACCC 42866, Lindnerella fabienne ACCC 43892, Pichia pastoris CICC 56783, Bacillus subtilis CICC 76836, Lactobacillus paracasei CICC 38763, Lactobacillus casei ACCC 32876 and Clostridium butyricum CICC 61836 is 5:5:5:5:2:1:1:0.3.

[0011] Furthermore, the anaerobic fermentation is carried out at 30°C-40°C in a closed static culture device filled with nitrogen.

[0012] The camphor tree leaf-vinasse culture medium is composed of the following components:

[0013] 50-150 parts by weight of camphor tree leaves

[0014] 500-700 parts by weight of distiller's grains

[0015] 30-70 parts by weight of corn flour

[0016] 30-70 parts by weight of soybean meal

[0017] 30-70 parts by weight of molasses

[0018] 5-15 parts by weight of potato flour

[0019] 10-30 parts by weight of baking soda

[0020] 2-6 parts by weight of leavening agent

[0021] 100-150 parts by weight of distilled water.

[0022] In one aspect, the present invention also provides a fermentation product.

[0023] A fermentation product is produced by the above-mentioned fermentation method.

[0024] In one aspect, the present invention also provides a novel yeast culture feed.

[0025] A novel yeast culture feed comprises the above-mentioned fermentation product.

[0026] Principle of the invention:

[0027] Distiller's grains, with their rich nutritional content and high yield, can be used as an unconventional raw material to compound specialized culture substrates. After fermentation using the process of the present invention, a distiller's grains-based yeast culture is obtained. Distiller's grains-based yeast culture is a green feed additive, rich in nutrients such as lactic acid, minerals, B vitamins, digestive enzymes, growth factors, a relatively complete range of amino acids, and unknown growth factors. It is a composite functional feed additive that combines nutrition and health benefits. It is inherently drug-resistant and residue-free, and has a wide range of applications in the feed industry, primarily in ruminant and aquatic feeds. In recent years, its application in livestock and poultry feeds has also been gradually recognized and promoted.

[0028] Yibin boasts approximately 70% of the world's unique camphor tree plant resources, producing nearly 600,000 tons of camphor leaf waste annually after essential oil extraction. Currently, this waste is primarily processed as fertilizer, resulting in low added value. However, analysis of its nutritional composition reveals 11.25% protein, 3.97% fat, 4.44% moisture, and 7.23% ash, demonstrating its potential for development as a bio-fermented feed. This patented invention can replace some roughage for ruminants (cattle, sheep, pigs, chickens, and fish), and can be combined with Sichuan's abundant distiller's grains to produce a novel yeast culture feed ingredient through fermentation using a specific composite strain of selected bacteria. This yeast culture product, a biogeographically designated product, effectively replaces staple crops like corn and soybean meal, alleviating competition for food between humans and livestock.

[0029] Compared with the prior art, the present invention has the following beneficial effects:

[0030] The present invention uses camphor leaves and distiller's grains as the basic culture medium for composite yeast, Bacillus subtilis, lactic acid bacteria and Clostridium butyricum, and through a low-temperature drying process, the fermentation metabolites are retained to a large extent, and a large number of bacterial protein cells are obtained, with an overall protein content of about 17%. It can effectively replace part of corn and soybean meal, such as replacing about 10-25% of corn or 5-15% of soybean meal, thereby obtaining a new yeast culture feed raw material with high application efficacy. The optimal addition ratio of the present invention's formula to replace corn in complete feed is 10%-25%, and the optimal addition ratio in pig feed is 10%. In addition, the present invention's formula is combined with corn, bran and soybean meal or rapeseed meal or detoxified cottonseed meal to form a pelleted feed, which avoids picky eating for feeding piglets, fattening pigs, mutton sheep, beef cattle, dairy cows and geese, ensuring the production performance and health of the animals.

[0031] The composite bacteria of the present invention have beneficial effects on the physical health of animals and have a synergistic effect. They can not only regulate the structure and balance of beneficial microbial flora in the gastrointestinal tract of animals and antagonize pathogenic bacteria, but also produce a large amount of substances that promote the conversion, absorption and utilization of feed by animals and the deposition of nutrients, thereby utilizing the conversion of animals and reducing the cost of meat production.

[0032] The invention adopts camphor tree and distiller's grains as fermentation raw materials and adopts the composite bacteria of the invention to prepare the yeast culture of the invention, thereby reducing feed costs, improving feed quality, and effectively alleviating the problem of competition for food between humans and animals. BRIEF DESCRIPTION OF THE DRAWINGS

[0033] Figure 1 It is a process flow chart of the present invention. DETAILED DESCRIPTION

[0034] The present invention will be further described below.

[0035] In the present invention, unless otherwise specified, % refers to mass percentage.

[0036] The formula of the present invention is made into finished products Figure 1 It should be noted that, in the following specific embodiments, the formulations compared with the formulations of the present invention are all the same except for the differences in the following table. Figure 1 In addition, in the present invention, each bacterial species in the composite bacterial formula is cultured separately and then inoculated into the culture medium.

[0037] The strains used in the following examples, Saccharomyces cerevisiae ACCC 41823, Candida utilis tropicalis ACCC 42866, Lindnerella fabienne ACCC 43892, Pichia pastoris CICC 56783 and Bacillus subtilis CICC 76836, Lactobacillus paracasei CICC 38763, Lactobacillus casei ACCC 32876 and Clostridium butyricum CICC 61836 were purchased from the China Agricultural Culture Collection Center (ACCC) and the China Industrial Culture Collection Center (CICC), and were confirmed to be correct by 16S sequence amplification and comparison.

[0038] Example 1 Culture Medium Comparison

[0039] The culture medium formula is as follows: Figure 1 The finished product produced by the process shown in the following table has the following formula components and process conditions. The finished product was tested for protein content, crude fiber content, β-mannose content, fat and Bacillus subtilis count, and the test results are shown in the following table 2.

[0040] Table 1

[0041]

[0042]

[0043] In Table 1, culture medium formula 3 is the culture medium formula of the present invention.

[0044] Table 2

[0045]

[0046] Example 2

[0047] Take the culture medium formula 3 of Example 1, according to Figure 1 The process steps were followed to compare different strains. The strain formula composition and process conditions are shown in Table 3 below. The finished products were tested for protein content, crude fiber content, β-glycan content, and fat content. The test results are shown in Table 4 below.

[0048] Table 3

[0049]

[0050]

[0051] In Table 3, strain formula 4 is the strain formula of the present invention.

[0052] Table 4

[0053]

[0054] Example 3

[0055] Take the culture medium formula 3 of Example 1 and the strain formula 4 of Example 2, according to Figure 1 The process steps were compared, and the incubation time (actual fermentation) comparison experiment was carried out. The finished products produced were tested, and the test results are shown in Table 5 below.

[0056] Table 5

[0057] Cultivation time 24h 48h 72h 96h Protein content 14.33％ 14.72％ 17.42％ 16.32％ Crude fiber content 8.23％ 8.05％ 7.80％ 7.80％ β-Mannan content 0.56％ 0.66％ 0.81％ 0.81％ Fat content 1.92％ 1.93％ 1.93％ 1.93％

[0058] The foregoing description is merely a preferred embodiment of the present invention and is not intended to limit the present invention. Those skilled in the art will readily appreciate that various modifications and variations of the present invention are possible. Any modifications, equivalent substitutions, or improvements made within the spirit and principles of the present invention are intended to be within the scope of protection of the present invention.

Claims

1. A fermentation method for producing yeast culture using camphor leaves and distiller's grains, comprising the following steps: S1, culturing each bacterial species in the composite bacterial formula separately; S2, taking each strain cultured in S1, inoculating it into a camphor leaf-vintner's grains culture medium, and performing anaerobic fermentation to obtain a finished product, which is used to replace 10-25% of corn or 5-15% of soybean meal in a complete feed; The composite bacteria formula is Saccharomyces cerevisiae ACCC 41823, Candida utilis tropicalis ACCC 42866, Lindnerella fabienne ACCC 43892, Pichia pastoris CICC 56783, Bacillus subtilis CICC 76836, Lactobacillus paracasei CICC 38763, Lactobacillus casei ACCC 32876 and Clostridium butyricum CICC 61836; The camphor tree leaf-vinasse culture medium is composed of the following components: 50-150 parts by weight of camphor tree leaves 500-700 parts by weight of distiller's grains 30-70 parts by weight of corn flour 30-70 parts by weight of soybean meal 30-70 parts by weight of molasses 5-15 parts by weight of potato flour 10-30 parts by weight of baking soda 2-6 parts by weight of leavening agent 100-150 parts by weight of distilled water.

2. The fermentation method for producing yeast culture from camphor tree leaves and vinasse according to claim 1, characterized in that: The inoculation ratio of the yeast Saccharomyces cerevisiae ACCC 41823, Candida utilis tropicalis ACCC 42866, Lindnerella fabienne ACCC43892, Pichia pastoris CICC 56783, Bacillus subtilis CICC 76836, Lactobacillus paracasei CICC 38763, Lactobacillus casei ACCC 32876 and Clostridium butyricum CICC 61836 is 5:5:5:5:2:1:1:0.3, and the total inoculation amount is 5%.

3. The fermentation method of the camphor leaf-vinasse yeast culture according to any one of claims 1-2, characterized in that: The anaerobic fermentation is carried out at 30° C. to 40° C. in a closed static culture device filled with nitrogen.

4. A fermentation product, characterized in that The fermentation product is produced by the fermentation method according to any one of claims 1 to 3.

5. The fermentation product according to claim 4, characterized in that The protein content of the fermentation product is 17%.

6. A novel yeast culture feed, characterized in that: The novel yeast culture feed replaces part of corn and / or soybean meal in a traditional feed formula with the fermentation product described in any one of claims 4-5.

7. The novel yeast culture feed according to claim 6, characterized in that: The corn replaces 10-25%, and the soybean meal replaces 5-15%.

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