Temperature identification type nanofiber-based wound film and preparation method and application thereof

Abstract

The application discloses a temperature identification type nanofiber-based wound film and a preparation method and application thereof. The wound film comprises two layers, an upper layer and a lower layer. The upper layer is prepared by chelating Eu 3+ / Tb 3+ ions with carboxylated nanocellulose to prepare carboxylated nanocellulose with fluorescent color, and then ultraviolet polymerization is performed on the carboxylated nanocellulose, hydroxypropyl methyl cellulose and photocured hydrogel to obtain a temperature identification fiber film. The lower layer is prepared by chemically grafting polyethyleneimine with photodynamic responsiveness and dopamine with near-infrared responsiveness on the carboxylated nanocellulose respectively to prepare photodynamic responsive intelligent nanofiber and near-infrared responsive intelligent nanofiber, and then ultraviolet polymerization is performed on the photodynamic responsive intelligent nanofiber and the near-infrared responsive intelligent nanofiber and the photocured hydrogel to obtain a photothermal / photodynamic antibacterial film. The wound film can realize photothermal / photodynamic synergistic antibiosis and photothermal temperature monitoring at the same time under 808nm laser irradiation, and prevent overheat damage of wound tissue when the photothermal temperature is greater than 50 DEG C.

Description

Technical Field

[0001] This invention belongs to the field of biomass fiber modification technology, specifically relating to a temperature-recognition nanofiber-based wound membrane, its preparation method, and its application. Background Technology

[0002] The skin is the first line of defense for organisms, resisting physical, mechanical, chemical, and pathogenic microorganism invasions and maintaining homeostasis. When the integrity of the skin is compromised, wounds are easily infected by pathogens, causing chronic inflammation and delayed wound healing, and in severe cases, tissue necrosis and even death. Antibiotics are the main clinical treatment for pathogenic bacterial infections; however, due to the overuse of antibiotics in recent decades, drug-sensitive bacteria have continuously mutated, leading to the emergence and spread of bacterial resistance, causing more serious biosafety problems. Near-infrared (NIR) photothermal therapy (PTT) is a promising alternative to antibiotics. The localized high temperature caused by NIR leads to bacterial protein denaturation and membrane damage, ultimately resulting in bacterial cell death. It can effectively combat antibiotic-resistant bacteria and biofilms without inducing resistance. However, during PTT, the photothermal temperature needs to be maintained above 42°C to eliminate bacteria, and the loss of moisture and the instability of the NIR laser during photothermal therapy can cause the photothermal temperature to exceed 50°C, damaging normal tissue and causing serious side effects. Current temperature monitoring technology still mainly relies on thermal imaging devices, which are inconvenient for wound management. Furthermore, for full-thickness infected wounds, PTT treatment alone may not be able to eliminate bacteria that have colonized deep within the tissue. Summary of the Invention

[0003] The purpose of this invention is to provide a temperature-recognition nanofiber-based wound membrane, its preparation method, and its application. This wound membrane has temperature-induced fluorescence change properties and can monitor photothermal temperature while achieving photothermal and photodynamic synergistic antibacterial effects under 808nm laser irradiation, preventing overheating damage to wound tissue when the photothermal temperature is >50℃.

[0004] The above-mentioned objectives of the present invention are achieved through the following technical solutions:

[0005] A temperature-sensing nanofiber-based wound membrane includes an upper layer and a lower layer. The upper layer is a temperature-sensing fiber membrane, and the lower layer is a photothermal / photodynamic antibacterial membrane. Under irradiation with an 808nm laser, the temperature-sensing nanofiber-based wound membrane can achieve synergistic antibacterial effects of photothermal and photodynamic therapy while simultaneously monitoring the temperature, preventing overheating damage to wound tissue when the photothermal temperature is >50℃.

[0006] The temperature-sensing fiber membrane uses carboxylated nanocellulose as a matrix and chelates Eu... 3+ or Tb 3+Ions were used to prepare carboxylated nanocellulose with fluorescent color, and then simultaneously polymerized with hydroxypropyl methylcellulose and photocurable hydrogel under ultraviolet light to prepare a temperature-sensing fiber membrane that can monitor temperatures >50℃.

[0007] The photothermal / photodynamic antibacterial film is prepared by chemically grafting photodynamic-responsive polyethyleneimine and near-infrared-responsive dopamine onto carboxylated nanocellulose to prepare photodynamic-responsive smart nanofibers and near-infrared-responsive smart nanofibers, which are then subjected to ultraviolet light polymerization with photocurable hydrogels to obtain a photothermal / photodynamic antibacterial film that can coordinate photothermal and photodynamic antibacterial effects.

[0008] Preferably, the photocurable hydrogel is polyether F127 diacrylate.

[0009] Preferably, the mass ratio of the fluorescent carboxylated nanocellulose, hydroxypropyl methylcellulose, and photocurable hydrogel in the temperature-sensing fiber membrane is 3:0.6 to 2:0.2 to 1.

[0010] Preferably, the mass ratio of photodynamic responsive smart nanofibers, near-infrared responsive smart nanofibers, and photocurable hydrogel in the photothermal / photodynamic antibacterial film is 3:5:0.2-1.

[0011] Preferably, both the upper and lower layers are circular, with the diameter of the upper layer being one-third smaller than that of the lower layer, so that the temperature-sensing fiber membrane of the upper layer can be uniformly subjected to heat conduction from the photothermal / photodynamic antibacterial membrane of the lower layer.

[0012] A temperature-sensitive nanofiber-based wound membrane of the present invention is prepared by a method comprising the following steps:

[0013] S1. Preparation of carboxylated nanocellulose with fluorescent color: Carboxylated nanocellulose and europium nitrate hexahydrate or terbium nitrate pentahydrate are uniformly dispersed in water at a mass ratio of 1.5:3-5, stirred for 30-40 min, centrifuged to precipitate, and the precipitate is freeze-dried to obtain carboxylated nanocellulose with fluorescent color.

[0014] S2. Preparation of photodynamic responsive polyethyleneimine: Dihydroporphyrin e6 photosensitizer and polyethyleneimine were dispersed in water at a mass ratio of 0.5 to 2:10. After ultrasonic treatment, the mixture was reacted at 100°C for 10 to 12 hours. The resulting solution was dialyzed with water for 22 to 24 hours and then freeze-dried to obtain photodynamic responsive polyethyleneimine.

[0015] S3. Preparation of photodynamic responsive smart nanofibers: Carboxylated nanocellulose and photodynamic responsive polyethyleneimine were dispersed in water at a mass ratio of 1:1 to 2.7. After ultrasonic treatment, the mixture was reacted at 100°C for 11 to 12 hours. After centrifugation and washing, the precipitate was freeze-dried to obtain photodynamic responsive smart nanofibers.

[0016] S4. Near-infrared responsive smart nanofibers: Carboxylated nanocellulose and dopamine were dispersed in water at a mass ratio of 1:2 to 4.3, ultrasonically treated, reacted at 100°C for 20 to 22 hours, centrifuged and washed, and then the precipitate was freeze-dried to obtain near-infrared responsive smart nanofibers.

[0017] S5. Preparation of temperature-recognition nanofiber-based wound membrane: Fluorescent carboxylated nanocellulose, hydroxypropyl methylcellulose, and polyether F127 diacrylate were dispersed in water at a mass ratio of 3:0.6–2:0.2–1. After ultrasonic treatment, the mixture was irradiated under ultraviolet light for 5 minutes to obtain a temperature-recognition fiber membrane. Photodynamic responsive smart nanofibers, near-infrared responsive smart nanofibers, and polyether F127 diacrylate were dispersed in water at a mass ratio of 3:5:0.2–1. After ultrasonic treatment, the mixture was irradiated under ultraviolet light for 5 minutes to obtain a photothermal / photodynamic antibacterial membrane. Then, the temperature-recognition fiber membrane was used as the upper layer, and the photothermal / photodynamic antibacterial membrane was used as the lower layer. The upper temperature-recognition fiber membrane was used to adhere itself to the lower photothermal / photodynamic antibacterial membrane to obtain a temperature-recognition nanofiber-based wound membrane.

[0018] The temperature-recognition nanofiber-based wound membrane of the present invention can be used for the treatment of infected wounds. When used for the treatment of infected wounds, it can rapidly heat up to 45°C and release active oxygen under 808nm laser irradiation, thereby killing bacteria through thermal ablation and oxidation. It exerts a synergistic antibacterial effect of photothermal and photodynamic therapy to promote wound healing. At the same time, it monitors the photothermal temperature during laser irradiation to prevent overheating damage to wound tissue when the temperature is >50°C.

[0019] The present invention has the following beneficial effects:

[0020] (1) The wound membrane prepared by the present invention comprises two layers: an upper layer is a temperature-sensing fiber membrane and a lower layer is a photothermal / photodynamic antibacterial membrane. Hydroxypropyl methylcellulose, as one of the components of the upper temperature-sensing fiber membrane, has different hydrophilicity and hydrophobicity at different temperatures. At >50℃, hydroxypropyl methylcellulose has high hydrophobicity (contact angle >121°), which reduces the distance between the carboxylated nanocellulose nanofibers with fluorescent color and significantly enhances the fluorescence, thereby giving the fiber membrane temperature-sensing properties. The lower photothermal / photodynamic antibacterial membrane is in contact with the wound. The photothermal / photodynamic antibacterial membrane contains both photodynamic responsive smart nanofibers and near-infrared responsive smart nanofibers. The near-infrared responsive smart nanofibers can exhibit a heating effect under 808nm laser stimulation, which can thermally ablate bacteria and biofilms in the wound bed. The photodynamic responsive smart nanofibers can release active oxygen under 808nm laser stimulation. The free active oxygen can penetrate into the deep tissue of the skin, thereby oxidizing and killing bacteria colonizing the deep tissue.

[0021] (2) Under irradiation with an 808nm laser, the lower photothermal / photodynamic antibacterial membrane of the wound membrane prepared by the present invention can achieve photothermal and photodynamic synergistic antibacterial and eliminate bacterial biofilm. At the same time, when the photothermal temperature is >50℃, the upper temperature recognition fiber membrane will show obvious red or green fluorescence, thereby prompting us to reduce or turn off the laser to prevent overheating damage to the wound tissue when the photothermal temperature is >50℃, thus realizing temperature monitoring while treating the wound. Attached Figure Description

[0022] Figure 1 This is a schematic diagram of the structure of a temperature-sensing nanofiber-based wound membrane according to the present invention.

[0023] Attached image labels: 1-upper layer, 2-lower layer. Detailed Implementation

[0024] like Figure 1 As shown, a temperature-recognition nanofiber-based wound membrane of the present invention includes an upper layer 1 and a lower layer 2, wherein the upper layer 1 is a temperature-recognition fiber membrane and the lower layer 2 is a photothermal / photodynamic antibacterial membrane.

[0025] Example 1

[0026] The preparation of a temperature-sensitive nanofiber-based wound membrane includes the following steps:

[0027] S1. Preparation of carboxylated nanocellulose with fluorescent color: 1.5000g of carboxylated nanocellulose and 3g of europium nitrate hexahydrate were uniformly dispersed in 30mL of water, stirred for 30min, centrifuged to precipitate, and the precipitate was freeze-dried to obtain carboxylated nanocellulose with fluorescent color.

[0028] S2. Preparation of photodynamic responsive polyethyleneimine: 50 mg of dihydroporphyrin e6 photosensitizer and 1 g of polyethyleneimine were dispersed in 100 mL of water, sonicated for 10 min, reacted at 100 °C with stirring for 10 h, and then quenched with liquid nitrogen. The resulting solution was dialyzed in water for 22 h using a MWCO500D dialysis bag, and then freeze-dried to obtain photodynamic responsive polyethyleneimine.

[0029] S3. Preparation of photodynamic responsive smart nanofibers: 1g of carboxylated nanocellulose and 1g of photodynamic responsive polyethyleneimine were dispersed in 200mL of water, sonicated for 15min, and reacted at 100℃ with stirring for 11h. After centrifugation and washing, the precipitate was freeze-dried to obtain photodynamic responsive smart nanofibers.

[0030] S4. Near-infrared responsive smart nanofibers: 1g of carboxylated nanocellulose and 2g of dopamine were dispersed in 200mL of water, sonicated for 10min, and then reacted at 100℃ with stirring for 20h. After centrifugation and washing, the precipitate was freeze-dried to obtain near-infrared responsive smart nanofibers.

[0031] S5. Preparation of temperature-recognition nanofiber-based wound membrane: 3g of fluorescent carboxylated nanocellulose, 0.6g of hydroxypropyl methylcellulose, and 0.2g of polyether F127 diacrylate were dispersed in 10mL of water. After ultrasonic treatment for 20min, the mixture was poured into a mold and irradiated under ultraviolet light for 5min to obtain a temperature-recognition fiber membrane; 3g of photodynamic-responsive smart nanofibers, 5g of near-infrared-responsive smart nanofibers, and 0.2g of polyether F127 diacrylate were dispersed in 10mL of water. In the process, after ultrasonic treatment for 20 minutes, the material is poured into a mold and then irradiated under ultraviolet light for 5 minutes to obtain a photothermal / photodynamic antibacterial membrane. Then, a temperature-sensing fiber membrane is used as the upper layer and the photothermal / photodynamic antibacterial membrane is used as the lower layer. The temperature-sensing fiber membrane is directly adhered to the lower photothermal / photodynamic antibacterial membrane by utilizing the adhesiveness of the upper layer to obtain a temperature-sensing nanofiber-based wound membrane. Both the upper and lower layers are circular, and the diameter of the upper temperature-sensing fiber membrane is one-third smaller than the diameter of the lower photothermal / photodynamic antibacterial membrane.

[0032] Example 2

[0033] The preparation of a temperature-sensitive nanofiber-based wound membrane includes the following steps:

[0034] S1. Preparation of carboxylated nanocellulose with fluorescent color: 1.5000g of carboxylated nanocellulose and 4g of europium nitrate hexahydrate were uniformly dispersed in 35mL of water, stirred for 30min, centrifuged to precipitate, and the precipitate was freeze-dried to obtain carboxylated nanocellulose with fluorescent color.

[0035] S2. Preparation of photodynamic responsive polyethyleneimine: 100 mg of dihydroporphyrin e6 photosensitizer and 1 g of polyethyleneimine were dispersed in 100 mL of water, sonicated for 10 min, reacted at 100 °C under stirring for 11 h, and then quenched with liquid nitrogen. The resulting solution was dialyzed in water for 23 h using a MWCO500D dialysis bag, and then freeze-dried to obtain photodynamic responsive polyethyleneimine.

[0036] S3. Preparation of photodynamic responsive smart nanofibers: 1g of carboxylated nanocellulose and 2g of photodynamic responsive polyethyleneimine were dispersed in 250mL of water, sonicated for 15min, and then reacted at 100℃ with stirring for 11.5h. After centrifugation and washing, the precipitate was freeze-dried to obtain photodynamic responsive smart nanofibers.

[0037] S4. Near-infrared responsive smart nanofibers: 1g of carboxylated nanocellulose and 3g of dopamine were dispersed in 200mL of water, sonicated for 10min, and then reacted at 100℃ with stirring for 21h. After centrifugation and washing, the precipitate was freeze-dried to obtain near-infrared responsive smart nanofibers.

[0038] S5. Preparation of temperature-recognition nanofiber-based wound membrane: 3g of fluorescent carboxylated nanocellulose, 1g of hydroxypropyl methylcellulose, and 0.5g of polyether F127 diacrylate were dispersed in 10mL of water. After ultrasonic treatment for 20min, the mixture was poured into a mold and then irradiated under ultraviolet light for 5min to obtain a temperature-recognition fiber membrane; 3g of photodynamic-responsive smart nanofibers, 5g of near-infrared-responsive smart nanofibers, and 0.5g of polyether F127 diacrylate were dispersed in 10mL of water. In the process, after ultrasonic treatment for 20 minutes, the material is poured into a mold and then irradiated under ultraviolet light for 5 minutes to obtain a photothermal / photodynamic antibacterial membrane. Then, a temperature-sensing fiber membrane is used as the upper layer and the photothermal / photodynamic antibacterial membrane is used as the lower layer. The temperature-sensing fiber membrane is directly adhered to the lower photothermal / photodynamic antibacterial membrane by utilizing the adhesiveness of the upper layer to obtain a temperature-sensing nanofiber-based wound membrane. Both the upper and lower layers are circular, and the diameter of the upper temperature-sensing fiber membrane is one-third smaller than the diameter of the lower photothermal / photodynamic antibacterial membrane.

[0039] Example 3

[0040] The preparation of a temperature-sensitive nanofiber-based wound membrane includes the following steps:

[0041] S1. Preparation of carboxylated nanocellulose with fluorescent color: 1.5000g of carboxylated nanocellulose and 5g of terbium nitrate pentahydrate were uniformly dispersed in 50mL of water, stirred for 30min, centrifuged to precipitate, and the precipitate was freeze-dried to obtain carboxylated nanocellulose with fluorescent color.

[0042] S2. Preparation of photodynamic responsive polyethyleneimine: 200 mg of dihydroporphyrin e6 photosensitizer and 1 g of polyethyleneimine were dispersed in 120 mL of water, sonicated for 10 min, reacted at 100 °C under stirring for 12 h, and then quenched with liquid nitrogen. The resulting solution was dialyzed in water for 24 h using a MWCO500D dialysis bag, and then freeze-dried to obtain photodynamic responsive polyethyleneimine.

[0043] S3. Preparation of photodynamic responsive smart nanofibers: 1g of carboxylated nanocellulose and 2.7g of photodynamic responsive polyethyleneimine were dispersed in 300mL of water, sonicated for 15min, and then reacted at 100℃ with stirring for 12h. After centrifugation and washing, the precipitate was freeze-dried to obtain photodynamic responsive smart nanofibers.

[0044] S4. Near-infrared responsive smart nanofibers: 1g of carboxylated nanocellulose and 4.3g of dopamine were dispersed in 250mL of water, sonicated for 10min, and then reacted at 100℃ with stirring for 22h. After centrifugation and washing, the precipitate was freeze-dried to obtain near-infrared responsive smart nanofibers.

[0045] S5. Preparation of temperature-recognition nanofiber-based wound membrane: 3g of fluorescent carboxylated nanocellulose, 2g of hydroxypropyl methylcellulose, and 1g of polyether F127 diacrylate were dispersed in 10mL of water. After ultrasonic treatment for 20min, the mixture was poured into a mold and then irradiated under ultraviolet light for 5min to obtain a temperature-recognition fiber membrane; 3g of photodynamic-responsive smart nanofibers, 5g of near-infrared-responsive smart nanofibers, and 1g of polyether F127 diacrylate were dispersed in 10mL of water. After ultrasonic treatment for 20 minutes, the mixture is poured into a mold and then irradiated under ultraviolet light for 5 minutes to obtain a photothermal / photodynamic antibacterial membrane. Then, a temperature-sensing fiber membrane is used as the upper layer and the photothermal / photodynamic antibacterial membrane is used as the lower layer. The temperature-sensing fiber membrane is directly adhered to the lower photothermal / photodynamic antibacterial membrane using the adhesiveness of the upper layer to obtain a temperature-sensing nanofiber-based wound membrane. Both the upper and lower layers are circular, and the diameter of the upper temperature-sensing fiber membrane is one-third smaller than the diameter of the lower photothermal / photodynamic antibacterial membrane.

[0046] Performance testing of temperature-sensing nanofiber-based wound membranes prepared in Examples 1, 2, and 3

[0047] ① The temperature-recognition nanofiber-based wound membranes prepared in Examples 1, 2, and 3 were tested for their temperature-stimulation response performance. The test results showed that the membranes exhibited strong red or green fluorescence under ultraviolet light at 50°C. When the wound membranes were applied to mouse wounds and irradiated with near-infrared laser, the thermal imager showed that the wound membranes could rapidly heat up to 45.7°C. When the temperature rose to 50°C, the wound membranes exhibited corresponding strong red or green fluorescence under ultraviolet light.

[0048] ② The temperature-recognition nanofiber-based wound membranes prepared in Examples 1, 2, and 3 were tested for in vitro antibacterial, antibiofilm, and in vivo anti-infection properties. The test results all showed good antibacterial properties. Under near-infrared laser irradiation, the wound membranes achieved antibacterial rates of over 99% against Escherichia coli, Staphylococcus aureus, and drug-resistant Staphylococcus aureus, and had a significant destructive ability against bacterial biofilms formed by Staphylococcus aureus. The in vivo anti-infection test results showed that the wound membranes could achieve a synergistic anti-infection effect of photothermal and photodynamic therapy under near-infrared laser stimulation. After a 14-day treatment process, the healing rate of infected wounds in mice was 99%. When the photothermal temperature was >50℃, the upper temperature-recognition fiber membrane showed obvious red or green fluorescence, thus providing a temperature warning and preventing secondary damage to the wound caused by photothermal temperatures >50℃. This enabled temperature monitoring while treating the wound.

Claims

1. A temperature-identifying nanofiber-based wound film, characterized by, It consists of an upper layer and a lower layer. The upper layer is a temperature-sensing fiber membrane, and the lower layer is a photothermal / photodynamic antibacterial membrane. Under irradiation with an 808nm laser, the temperature-sensing nanofiber-based wound membrane can achieve synergistic antibacterial effects of photothermal and photodynamic therapy while monitoring the temperature, preventing overheating damage to the wound tissue when the photothermal temperature is >50℃. The temperature-sensing fiber membrane uses carboxylated nanocellulose as a matrix and chelates Eu... 3+ or Tb 3+ Ions were used to prepare carboxylated nanocellulose with fluorescent color, and then simultaneously polymerized with hydroxypropyl methylcellulose and photocurable hydrogel under ultraviolet light to prepare a temperature-sensing fiber membrane that can monitor temperatures >50℃. The photothermal / photodynamic antibacterial film is prepared by chemically grafting photodynamic-responsive polyethyleneimine and near-infrared-responsive dopamine onto carboxylated nanocellulose to prepare photodynamic-responsive smart nanofibers and near-infrared-responsive smart nanofibers, which are then polymerized with photocurable hydrogel under ultraviolet light to obtain a photothermal / photodynamic antibacterial film that can coordinate photothermal and photodynamic antibacterial properties. The photocurable hydrogel is polyether F127 diacrylate; The mass ratio of fluorescent carboxylated nanocellulose, hydroxypropyl methylcellulose, and photocurable hydrogel in the temperature-sensing fiber membrane is 3:0.6~2:0.2~1. The mass ratio of photodynamic responsive smart nanofibers, near-infrared responsive smart nanofibers, and photocurable hydrogel in the photothermal / photodynamic antibacterial film is 3:5:0.2~1. The photodynamic responsive polyethyleneimine is prepared by dispersing dihydroporphyrin e6 photosensitizer and polyethyleneimine in water at a mass ratio of 0.5~2:10, sonicating, reacting at 100℃ for 10~12h, dialyzing the obtained solution with water for 22~24h, and then freeze-drying to obtain the photodynamic responsive polyethyleneimine.

2. The temperature-identifying nanofiber-based wound film according to claim 1, wherein, Both the upper and lower layers are circular, with the diameter of the upper layer being one-third smaller than that of the lower layer.

3. The temperature-sensitive nanofiber-based wound membrane according to claim 1, characterized in that, The method includes the following steps to prepare: S1. Preparation of carboxylated nanocellulose with fluorescent color: Carboxylated nanocellulose and europium nitrate hexahydrate or terbium nitrate pentahydrate are uniformly dispersed in water at a mass ratio of 1.5:3~5, stirred for 30~40 min, centrifuged to precipitate, and the precipitate is freeze-dried to obtain carboxylated nanocellulose with fluorescent color. S2. Preparation of photodynamic responsive polyethyleneimine: Dihydroporphyrin e6 photosensitizer and polyethyleneimine were dispersed in water at a mass ratio of 0.5~2:10, ultrasonicated, and reacted at 100℃ for 10~12h. The obtained solution was dialyzed with water for 22~24h and then freeze-dried to obtain photodynamic responsive polyethyleneimine. S3. Preparation of photodynamic responsive smart nanofibers: Carboxylated nanocellulose and photodynamic responsive polyethyleneimine were dispersed in water at a mass ratio of 1:1~2.

7. After ultrasonic treatment, the mixture was reacted at 100℃ for 11~12h, centrifuged and washed, and then the precipitate was freeze-dried to obtain photodynamic responsive smart nanofibers. S4. Near-infrared responsive smart nanofibers: Carboxylated nanocellulose and dopamine were dispersed in water at a mass ratio of 1:2~4.3, ultrasonically treated, reacted at 100℃ for 20~22h, centrifuged and washed, and then the precipitate was freeze-dried to obtain near-infrared responsive smart nanofibers. S5. Preparation of temperature-recognition nanofiber-based wound membrane: Fluorescent carboxylated nanocellulose, hydroxypropyl methylcellulose, and polyether F127 diacrylate were dispersed in water at a mass ratio of 3:0.6~2:0.2~1. After ultrasonic treatment, the mixture was irradiated under ultraviolet light for 5 minutes to obtain a temperature-recognition fiber membrane. Photodynamic responsive smart nanofibers, near-infrared responsive smart nanofibers, and polyether F127 diacrylate were dispersed in water at a mass ratio of 3:5:0.2~1. After ultrasonic treatment, the mixture was irradiated under ultraviolet light for 5 minutes to obtain a photothermal / photodynamic antibacterial membrane. Then, the temperature-recognition fiber membrane was used as the upper layer, and the photothermal / photodynamic antibacterial membrane was used as the lower layer. The upper temperature-recognition fiber membrane was used to adhere itself to the lower photothermal / photodynamic antibacterial membrane to obtain a temperature-recognition nanofiber-based wound membrane.

4. The application of the temperature-recognition nanofiber-based wound membrane according to any one of claims 1 to 3 in the preparation of an agent for treating infected wounds; during the treatment of infected wounds, the temperature-recognition nanofiber-based wound membrane can rapidly heat up to 45°C and release active oxygen under 808nm laser irradiation, thereby killing bacteria through thermal ablation and oxidation, exerting a synergistic antibacterial effect of photothermal and photodynamic therapy to promote wound healing, while monitoring the photothermal temperature during laser irradiation to prevent overheating damage to wound tissue when the temperature is >50°C.

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