Application of 4-methylcatechol in preparing herbicides for weeds

Abstract

The present invention belongs to the technical field of farmland weed control, and particularly relates to the application of 4-methylcatechol in the preparation of weed herbicides. The present invention discloses that 4-methylcatechol at a concentration of 0.01-9 g / L can inhibit the growth of Leptochloa chinensis, providing a possibility for the manufacture of a new plant-derived herbicide against Leptochloa chinensis.

Description

Technical Field

[0001] The present invention belongs to the technical field of farmland weed control, and particularly relates to the application of 4-methylcatechol in the preparation of weed herbicides. Background Art

[0002] With the large-scale popularization of the reduced tillage and no-tillage rice cultivation mode and direct-seeded rice, the gramineous upland weed, Leptochloa chinensis, has spread rapidly in paddy fields in southern China and has become one of the main malignant weeds in paddy fields, seriously affecting the yield and quality of rice. At present, the control of Leptochloa chinensis mainly relies on chemical herbicides. However, the long-term use of chemical herbicides has led to the emergence and development of herbicide-resistant Leptochloa chinensis, and the situation has become increasingly serious. There is a phenomenon of "no available herbicide" in some paddy fields. This not only poses a great challenge to the control of Leptochloa chinensis but also brings pressure to ecological environment protection. At present, the green development of agriculture has become the main theme. Developing new green herbicides can not only effectively alleviate the rapid development of resistant weeds but also promote the sustainable development of the rice industry.

[0003] Plant-derived herbicides have broad application prospects in the development of green agriculture and organic agriculture. Plant-derived active substances provide valuable resources for the development of plant-derived herbicides. 4-Methylcatechol is an important organic chemical raw material and belongs to one of the plant metabolites. It is mainly used as a preservative for photosensitive materials to play a role in sterilization and mildew prevention, and is also the main source of apple-type flavors, etc. CN115120521A discloses a polyphenol active composition, its preparation method and application, and discloses that 4-methylcatechol can be applied to industrial fields such as cosmetics, health products, and drugs for anti-inflammatory and soothing, acne removal and scar removal, wrinkle removal and anti-aging, etc. At present, there is no literature data and relevant reports on the control of Leptochloa chinensis in farmland weeds by 4-methylcatechol. Summary of the Invention

[0004] In order to provide a new plant-derived herbicidal substance, the present invention provides the following technical solutions:

[0005] The application of 4-methylcatechol in inhibiting weed growth.

[0006] Preferably, the weed is Leptochloa chinensis.

[0007] Preferably, the structural formula of the 4-methylcatechol is:

[0008]

[0009] Preferably, the concentration of the 4-methylcatechol is: 0.01 - 9 g / L.

[0010] The application of 4-methylcatechol in the preparation of weed herbicides.

[0011] Preferably, the weed is Leptochloa chinensis.

[0012] Preferably, the structural formula of the 4-methylcatechol is:

[0013]

[0014] Preferably, the concentration of the 4-methylcatechol is: 0.01-9 g / L.

[0015] A method for controlling plant weeds, the method is: spraying 3-9 g / L of 4-methylcatechol on the weed seedlings. Preferably, the weed is Leptochloa chinensis.

[0016] Advantages of the present invention:

[0017] The present invention discloses that using 0.01-9 g / L of 4-methylcatechol can inhibit the growth of Leptochloa chinensis, providing the possibility for manufacturing a new plant-derived herbicide against Leptochloa chinensis. Description of the drawings

[0018] Figure 1 To verify the effect of 4-methylcatechol at different concentrations on the growth of Leptochloa chinensis using the petri dish method.

[0019] Figure 2 To verify the effect of 4-methylcatechol at different concentrations on the growth of Leptochloa chinensis using the whole plant method.

[0020] Figure 3 Symptom diagram of Leptochloa chinensis under the treatment of 4-methylcatechol.

[0021] Figure 4 Fresh weight inhibition rate of Leptochloa chinensis under the treatment of 4-methylcatechol. Specific embodiments

[0022] To make the purpose, technical solution and advantages of the present invention clearer, the following will combine the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments in the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts belong to the scope of protection of the present invention.

[0023] The CAS of the 4-methylcatechol involved in the present invention is: 452-86-8.

[0024] Experiment 1:

[0025] 1. Gently grind the glumes of Leptochloa chinensis seeds with fine sandpaper, and then soak the seeds in pure water. After germinating at a low temperature (4 °C) for 24 h, dry the water with filter paper and set aside.

[0026] 2. Use pure water as solvent to prepare solutions of 0, 10, 50, 100, and 200 mg / L. Use the culture dish filter paper method, put about 50 seeds of Leptochloa chinensis in each dish in a culture dish covered with two layers of filter paper, add 5 mL of 4-methylcatechol aqueous solution of different concentrations, repeat 3 times for each treatment, use pure water treatment as control, culture in an incubator at a temperature of (30±1)℃, alternate light / darkness of 12h / 12h every day, and measure the germination rate, root length and stem length of Leptochloa chinensis after 72h.

[0027] 3. Test results such as Figure 1 As shown in Table 1-3:

[0028] Table 1 Effect of 4-methylcatechol on germination rate of Leptochloa chinensis

[0029] Concentration (mg / L) Germination rate (%) Inhibition rate (%) 0 65.43±9.54a 0 10 48.87±8.13b 24.62 50 38.23±9.34c 41.54 100 28.9±1.75bc 55.38 200 0.00±0.00d 100

[0030] Table 2 Effects of 4-methylcatechol on the bud growth of Leptochloa chinensis

[0031] Concentration (mg / L) Bud length (cm) Inhibition rate (%) 0 0.31±0.04a 0 10 0.25±0.04b 19.35 50 0.2±00.02b 35.48 100 0.19±0.03b 38.71 200 0.00±0.00c 100

[0032] Table 3 Effects of 4-methylcatechol on root length of Leptochloa chinensis

[0033] Concentration (mg / L) Root length (cm) Inhibition rate (%) 0 0.8±0.04a 0 10 0.22±0.04b 72.5 50 0.13±0.04c 83.75 100 0.00±0.00d 100 200 0.00±0.00d 100

[0034] As shown in Tables 1-3 above, 10 mg / L 4-methylcatechol can significantly inhibit the germination of Leptochloa chinensis, with an inhibition rate of 24.62%, and 200 mg / L 4-methylcatechol can completely inhibit the germination of Leptochloa chinensis. 10 mg / L 4-methylcatechol significantly inhibited the growth of the buds and roots of Leptochloa chinensis, especially the root growth of Leptochloa chinensis, with an inhibition rate of 72.5%. When Leptochloa chinensis was treated with 100 mg / L 4-methylcatechol, the roots could not grow. This shows that 4-methylcatechol has a high inhibitory effect on the germination and root growth of Leptochloa chinensis seeds, and has the potential to be developed as a new herbicide.

[0035] Test 2:

[0036] Grind off the husk of the seeds of the genus Leptochloa, add an appropriate amount of pure water to germinate in a constant temperature incubator, then transplant the germinated seeds into a suitable size culture pot, keep sufficient water, and culture them in a ventilated and light-permeable culture room to simulate the outdoor growth environment. When the Leptochloa genus grows to 3-4 leaves, thin out the seedlings and leave about 15 plants with uniform growth. Set the concentration of 4-methylcatechol solution (aqueous solution) to 0.5, 1.0, 3.0, 6.0, and 9.0 g / L and spray the Leptochloa genus seedlings. Repeat three treatments in each group and spray 10 ml. The results are as follows: Figure 2 The results of taking photos and recording them 3 days after spraying are shown in Figure 3As shown, after 5 days of treatment, the above-ground fresh weight of weeds in each treatment was weighed, and the fresh weight control effect was calculated. E1 = (C1 - T1) / C1 × 100%, where: E1 is the fresh weight control effect; C1 is the above-ground fresh weight of the control weeds; T1 is the above-ground fresh weight of the treated weeds. The results are as Figure 4 shown.

[0037] After applying 4-methylcatechol to Leptochloa chinensis, as Figure 2 , 3 shown, black spots appeared on the plant leaves, and the plants withered at high concentrations. Statistical data analysis found that 3 g / L of 4-methylcatechol could significantly inhibit the growth of Leptochloa chinensis seedlings, and its fresh weight control effect was 12.36%; the inhibition rate of 9 g / L of 4-methylcatechol on the fresh weight of Leptochloa chinensis was 71.89%, and the inhibitory effect was significantly higher than that of other concentration treatments.

Claims

1. Use of 4-methylcatechol in inhibiting weed growth, characterized in that, The weed is Leptochloa chinensis; The structural formula of the 4-methylcatechol is: 。 2. The use according to claim 1, characterized in that, The concentration of the 4-methylcatechol is: 0.01 - 9 g / L.

3. Use of 4-methylcatechol in preparing a weed herbicide, characterized in that, The weed is Leptochloa chinensis; The structural formula of the 4-methylcatechol is: 。 4. The use according to claim 3, characterized in that, The concentration of the 4-methylcatechol is: 0.01 - 9 g / L.

5. A method for controlling farmland weeds, characterized in that, The method is: spraying 3 - 9 g / L of 4-methylcatechol on the seedlings of plant weeds; the weed is Leptochloa chinensis.

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