A pathogenic bacteria detection magnetic bead quantitative adding device

Abstract

The application discloses a kind of pathogenic bacteria detection magnetic beads quantitative adding equipment, it is related to biological detection technical field, the magnetic beads quantitative adding equipment includes equipment machine case main body, equipment machine case main body is sequentially provided with pathogenic bacteria liquid centrifugal adding mechanism, intermittent magnetic bead adding mechanism and detection observation mechanism from top to bottom in it, pathogenic bacteria liquid centrifugal adding mechanism and intermittent magnetic bead adding mechanism are respectively linked with detection observation mechanism.This application is a kind of pathogenic bacteria detection magnetic beads quantitative adding equipment, by quantitative adding magnetic beads to absorb pathogenic bacteria body, to form pathogenic bacteria colony, to facilitate better detection pathogenic bacteria body, it adds pathogenic bacteria liquid after centrifugation using pathogenic bacteria liquid centrifugal adding mechanism, the content of pathogenic bacteria body in pathogenic bacteria liquid at this time will be high, after adding magnetic beads, more easily absorb pathogenic bacteria body, then form pathogenic bacteria colony;Pathogenic bacteria liquid and magnetic beads are directly added to detection observation mechanism, the absorption condition of pathogenic bacteria body by magnetic beads can be directly observed.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of biological detection, in particular to a pathogenic bacteria detection magnetic bead quantitative adding device. BACKGROUND

[0002] Superparamagnetic microspheres are referred to as magnetic beads. In recent years, superparamagnetic microspheres have been widely used in immunochromatography technology. By detecting biochemical substances combined on superparamagnetic nanometer microparticles, quantitative detection data of biological samples is provided, greatly improving the performance of traditional methods. The content of magnetic microspheres coupled with nucleic acids, antigens or antibodies is qualitatively or quantitatively measured by measuring the magnetic field strength of the detection area on the test strip. The concentration of immunocomplex and the magnetic field strength are made into a standard curve, so as to calculate the amount of the biological molecule to be measured. Superparamagnetic immunochromatography technology is widely used in clinical diagnosis, pesticide and veterinary drug residues, food inspection, microbial detection and other fields.

[0003] In the prior art, one patent with an application date of September 17, 2021 and an application publication number of CN 113866430A, "magnetic bead adding device, coagulation equipment and magnetic bead adding method", the magnetic bead adding device in the patent comprises: a magnetic bead storage body provided with a first storage area with a drop opening; and a bead adding module, the bead adding module includes a driving mechanism and an execution mechanism, the execution mechanism includes a plurality of execution units, each execution unit includes a transmission member, an execution assembly provided with a magnetic bead channel, and a coupling assembly for realizing the separable coupling of the transmission member and the execution assembly, the driving mechanism can drive all the transmission members to move together; wherein the drop opening is located above the moving path of all the magnetic bead channels.

[0004] However, the patent directly adds magnetic beads to the test cup during detection, at which time the pathogenic bacteria bodies in the test cup are distributed randomly, and it is not easy to quickly adsorb pathogenic bacteria bodies after adding magnetic beads. Moreover, the cost of magnetic beads is high, which not only slows down the adsorption speed, but also increases the consumption of magnetic beads. After adding the magnetic beads to the test cup, it is also necessary to correspondingly transfer them to the microscope for observation, which is very inconvenient in use. SUMMARY

[0005] The purpose of the present application is to solve the problems in the background art, and to provide a pathogenic bacteria detection magnetic bead quantitative adding device.

[0006] To achieve the above-mentioned purpose, the present application provides the following technical solutions:

[0007] The application discloses a pathogenic bacteria detection magnetic bead quantitative adding device which comprises a device cabinet main body, a pathogenic bacteria liquid centrifugal adding mechanism, an intermittent magnetic bead adding mechanism and a detection observation mechanism which are sequentially arranged in the device cabinet main body from top to bottom, and the pathogenic bacteria liquid centrifugal adding mechanism and the intermittent magnetic bead adding mechanism are connected with the detection observation mechanism.

[0008] By adopting the above scheme, when the pathogenic bacteria liquid centrifugal adding mechanism is applied, the pathogenic bacteria liquid is centrifuged, the centrifuged pathogenic bacteria liquid is added on the detection observation mechanism, the magnetic beads are sequentially added through the intermittent magnetic bead adding mechanism, and the pathogenic bacteria bodies are adsorbed on the pathogenic bacteria colonies by the magnetic beads until the detection observation mechanism is observed, so that the subsequent detection is facilitated.

[0009] Further, as a preferred scheme, the pathogenic bacteria liquid centrifugal adding mechanism comprises a pathogenic bacteria liquid centrifugal box which is detachably arranged in a ring-shaped centrifugal cavity which is arranged above the device cabinet main body, a sealing cover is threadedly assembled above the ring-shaped centrifugal cavity, and a centrifugal rotating seat is movably assembled below the ring-shaped centrifugal cavity, and the centrifugal rotating seat is in transmission connection with a rotating shaft of a driving motor.

[0010] Two centrifugal openings are formed in the two sides of the pathogenic bacteria liquid centrifugal box, and a one-way switch assembly is arranged at the centrifugal opening in a corresponding mode, and the one-way switch assembly is located outside the pathogenic bacteria liquid centrifugal box.

[0011] Two pathogenic bacteria liquid adding channels are in communication with the lower sides of the ring-shaped centrifugal cavity.

[0012] Two limiting heads are fixed to the upper surface of the centrifugal rotating seat, and the centrifugal rotating seat is limitingly assembled with the pathogenic bacteria liquid centrifugal box through the limiting heads.

[0013] The one-way switch assembly comprises a one-way switch valve cover which is arranged at the centrifugal opening in a corresponding mode, the two sides of the one-way switch valve cover are slidably assembled on a guide shaft one, a spring block one is fixed to the end of the guide shaft one, and a spring one is sleeved on the guide shaft one.

[0014] When the pathogenic bacteria liquid centrifugal adding mechanism works, the driving motor is started to drive the centrifugal rotating seat to rotate, so that the pathogenic bacteria liquid centrifugal box is rotated, the pathogenic bacteria liquid in the pathogenic bacteria liquid centrifugal box is under the action of centrifugal force, the one-way switch valve cover is opened, the centrifuged pathogenic bacteria liquid enters the ring-shaped centrifugal cavity from the centrifugal opening, and then is added into a standard measuring dish through the pathogenic bacteria liquid adding channel, and if the pathogenic bacteria liquid in the standard measuring dish overflows, the pathogenic bacteria liquid falls into a ring-shaped material groove for storage.

[0015] Further as preferred, the intermittent magnetic bead adding mechanism comprises an intermittent magnetic bead adding box which is detachably placed in a placing cavity I opened in the middle of the equipment cabinet main body, the front and back of the placing cavity I are connected with the outside, the bottom of the intermittent magnetic bead adding box is provided with an adding port, the adding port is provided with an intermittent switch assembly, and the intermittent switch assembly is located in the intermittent magnetic bead adding box.

[0016] The adding port is connected with an intermittent trigger cavity below, the intermittent trigger cavity is internally provided with an intermittent trigger assembly, and the intermittent trigger cavity is connected with a magnetic bead adding channel below.

[0017] The intermittent switch assembly comprises an intermittent switch valve core which is slidingly assembled on a guide shaft II, the end of the guide shaft II is fixed with a spring baffle II, and the guide shaft II is further sleeved with a spring II.

[0018] The intermittent trigger assembly comprises a trigger frame which is movably assembled in the intermittent trigger cavity, the upper end of the trigger frame corresponds to the upper and lower positions of the two intermittent switch valve cores respectively, the trigger frame is movably provided with a trigger wheel below, the lower end of the trigger frame is always connected with the surface of the trigger wheel, and the trigger wheel is in transmission connection with the rotating shaft of a driving motor fixed to the outside of the equipment cabinet main body.

[0019] The trigger wheel is provided with only one trigger tooth head, and the surface of the trigger tooth head and the lower end surface of the trigger frame are both arc surfaces.

[0020] The upper surface of the intermittent switch valve core is an inclined inclined surface.

[0021] Through the above scheme, when the intermittent magnetic bead adding mechanism works, the driving motor is started to drive the trigger wheel to rotate, under the action of the trigger tooth head, the trigger frame is abutted and moved upward, at this time, the intermittent switch valve core is opened again by the trigger frame, the magnetic beads in the intermittent magnetic bead adding box enter the intermittent trigger cavity through the adding port, and then are added to the standard measuring dish through the magnetic bead adding channel, at this time, the magnetic beads begin to adsorb the pathogenic bacteria and continuously approach.

[0022] Further as preferred, the detection and observation mechanism comprises a standard measuring dish which is detachably placed in a placing cavity II opened below the equipment cabinet main body, the front and back of the placing cavity II are connected with the outside, the outlets of the pathogenic bacteria liquid adding channel and the magnetic bead adding channel are both located above the standard measuring dish, and the lower part of the placing cavity II is provided with an annular material groove located outside the equipment cabinet main body.

[0023] The placing cavity II is provided with an observation cavity below, the observation cavity is internally fixed with an upward observation microscope, the microscope is fixed with an upward shooting camera below, and the camera is connected with a display screen fixed outside the equipment cabinet main body.

[0024] The standard measuring dish is made of fully visualized material, such as glass material.

[0025] By adopting the above scheme, when the detection observation mechanism is working, the condition that the magnetic beads adsorb pathogenic bacteria bodies in the standard measuring dish is observed in real time through the microscope, then the camera shoots the image picture under the microscope observation in real time, and the display screen displays in real time, since the amount of the magnetic beads added at a time is small, if it is observed that the magnetic beads adsorb pathogenic bacteria bodies and pathogenic bacteria colonies are not formed, the magnetic beads are added until the pathogenic bacteria colonies are formed.

[0026] Compared with the prior art, the beneficial effects of the application are:

[0027] 1. The pathogenic bacteria liquid centrifugal adding mechanism is used to add the pathogenic bacteria liquid after centrifugation, at this time, the content of the pathogenic bacteria bodies in the pathogenic bacteria liquid will be high, after the magnetic beads are added, the pathogenic bacteria bodies are more easily adsorbed, and then pathogenic bacteria colonies are formed;

[0028] 2. The pathogenic bacteria liquid and the magnetic beads are directly added to the detection observation mechanism, and the adsorption condition of the pathogenic bacteria bodies by the magnetic beads can be directly observed. BRIEF DESCRIPTION OF DRAWINGS

[0029] Figure 1 It is a front view structural schematic diagram of the application;

[0030] Figure 2 It is a left view structural schematic diagram of the application;

[0031] Figure 3 It is a top view structural schematic diagram of the application;

[0032] Figure 4 It is Figure 2 a sectional view along A-A in the figure;

[0033] Figure 5 It is Figure 4 a partial structural schematic diagram in the figure; Figure 1

[0034] Figure 6 It is Figure 5 a local enlarged view at B in the figure;

[0035] Figure 7 It is Figure 4 a partial structural schematic diagram in the figure; Figure 2

[0036] Figure 8 It is Figure 7 a local enlarged view at C in the figure;

[0037] Figure 9 It is Figure 4 a partial structural schematic diagram in the figure; Figure 3 .​​

[0038] In the figure: 1-device machine box body; 2-pathogenic bacteria liquid centrifugal box; 3-annular centrifugal cavity; 4-sealing cover; 5-centrifugal rotating seat; 6-driving motor; 7-limiting head; 8-centrifugal port; 9-one-way switch valve cover; 10-guiding shaft one; 11-spring stopper one; 12-spring one; 13-intermittent magnetic bead adding box; 14-placing cavity one; 15-adding port; 16-intermittent switch valve core; 17-guiding shaft two; 18-spring stopper two; 19-spring two; 20-intermittent trigger cavity; 21-trigger frame; 22-trigger wheel; 23-driving motor; 24-trigger tooth head; 25-magnetic bead adding channel; 26-standard measuring dish; 27-placing cavity two; 28-annular material groove; 29-observation cavity; 30-microscope; 31-camera; 32-display screen; 33-pathogenic bacteria liquid adding channel. DETAILED DESCRIPTION

[0039] The technical solutions in the embodiments of the application will be clearly and completely described below with reference to the drawings in the embodiments of the application. Obviously, the described embodiments are only part of the embodiments of the application, rather than all the embodiments of the application. Based on the embodiments in the application, all other embodiments obtained by a person of ordinary skill in the art without creative work fall within the protection scope of the application.

[0040] Please refer to Figures 1-9 The application provides a technical solution:

[0041] The magnetic bead quantitative adding equipment for pathogenic bacteria detection comprises a device machine box body 1, a pathogenic bacteria liquid centrifugal adding mechanism, an intermittent magnetic bead adding mechanism and a detection observation mechanism are sequentially arranged in the device machine box body 1 from top to bottom, the pathogenic bacteria liquid centrifugal adding mechanism and the intermittent magnetic bead adding mechanism are respectively connected to the detection observation mechanism, and the condition that the magnetic beads adsorb the pathogenic bacteria liquid can be directly observed through the detection observation mechanism.

[0042] In application, the pathogenic bacteria liquid is centrifuged through the pathogenic bacteria liquid centrifugal adding mechanism, the centrifuged pathogenic bacteria liquid is added on the detection observation mechanism, the magnetic beads are sequentially added through the intermittent magnetic bead adding mechanism, and the pathogenic bacteria bodies are adsorbed into pathogenic bacteria colonies through the detection observation mechanism, so that the subsequent detection is facilitated.

[0043] Specifically, the pathogenic bacteria liquid centrifugal adding mechanism comprises a pathogenic bacteria liquid centrifugal box 2, the pathogenic bacteria liquid centrifugal box 2 is detachably placed in an annular centrifugal cavity 3 formed above the device machine box body 1, a sealing cover 4 is threadedly assembled above the annular centrifugal cavity 3, a centrifugal rotating seat 5 is movably assembled below the annular centrifugal cavity 3, and the centrifugal rotating seat 5 is in transmission connection with the rotating shaft of a driving motor 6.

[0044] The pathogenic bacteria liquid centrifugal box 2 is provided with centrifugal openings 8 on both sides, and a one-way switch assembly is arranged at the centrifugal openings 8.

[0045] The lower sides of the annular centrifugal cavity 3 are communicated with pathogenic bacteria liquid adding channels 33.

[0046] The upper surface of the centrifugal rotating seat 5 is fixed with two limiting heads 7, and the centrifugal rotating seat 5 is assembled with the pathogenic bacteria liquid centrifugal box 2 through the limiting heads 7.

[0047] The one-way switch assembly comprises a one-way switch valve cover 9, which is arranged at the centrifugal openings 8 and is slidably assembled on a guide shaft 10 on both sides, the end of the guide shaft 10 is fixed with a spring stopper 11, and the guide shaft 10 is further sleeved with a spring 12.

[0048] When the pathogenic bacteria liquid centrifugal adding mechanism works, the driving motor 6 is started to drive the centrifugal rotating seat 5 to rotate, so that the pathogenic bacteria liquid centrifugal box 2 rotates, and the pathogenic bacteria liquid in the pathogenic bacteria liquid centrifugal box 2 is opened under the action of the centrifugal force, at this time, the centrifuged pathogenic bacteria liquid enters the annular centrifugal cavity 3 from the centrifugal openings 8, and then is added to the standard measuring dish 26 through the pathogenic bacteria liquid adding channel 33, if the pathogenic bacteria liquid in the standard measuring dish 26 overflows, it will flow into the annular material groove 28 for storage.

[0049] Specifically, the intermittent magnetic bead adding mechanism comprises an intermittent magnetic bead adding box 13, which is detachably placed in a placing cavity 14 arranged in the middle of the equipment cabinet main body 1, the front and rear of the placing cavity 14 are connected with the outside, and the bottom of the intermittent magnetic bead adding box 13 is provided with an adding opening 15, and the adding opening 15 is provided with an intermittent switch assembly, and the intermittent switch assembly is located in the intermittent magnetic bead adding box 13.

[0050] The lower side of the adding opening 15 is communicated with an intermittent trigger cavity 20, the intermittent trigger cavity 20 is internally provided with an intermittent trigger assembly, and the lower side of the intermittent trigger cavity 20 is communicated with a magnetic bead adding channel 25.

[0051] The intermittent switch assembly comprises an intermittent switch valve core 16, which is slidably assembled on a guide shaft 17, the end of the guide shaft 17 is fixed with a spring stopper 18, and the guide shaft 17 is further sleeved with a spring 19.

[0052] The intermittent trigger assembly comprises a trigger frame 21 movably assembled in the intermittent trigger cavity 20, the upper end of the trigger frame 21 corresponds to the upper and lower positions of the intermittent switch valve core 16 respectively, the lower part of the trigger frame 21 movably assembled with a trigger wheel 22, the lower end of the trigger frame 21 is always in contact with the surface of the trigger wheel 22, and the trigger wheel 22 is in transmission connection with the rotating shaft of the driving motor 23 fixedly assembled on the outer side of the equipment cabinet main body 1.

[0053] The trigger wheel 22 is provided with only one trigger tooth head 24, and the surface of the trigger tooth head 24 and the lower end surface of the trigger frame 21 are both arc surfaces.

[0054] The upper surface of the intermittent switch valve core 16 is an inclined inclined surface.

[0055] When the intermittent magnetic bead adding mechanism works, the driving motor 23 is started to drive the trigger wheel 22 to rotate, under the action of the trigger tooth head 24, the trigger frame 21 is abutted and moved upward, at this time, the intermittent switch valve core 16 is opened again by the trigger frame 21, the magnetic beads in the intermittent magnetic bead adding box 13 enter the intermittent trigger cavity 20 through the adding port 15, and then are added to the standard measuring dish 26 through the magnetic bead adding channel 25, at this time, the magnetic beads begin to adsorb the pathogenic bacteria bodies and continuously approach.

[0056] Specifically, the detection and observation mechanism comprises a standard measuring dish 26 which is detachably placed in a placing cavity two 27 opened in the lower part of the equipment cabinet main body 1, the front and rear of the placing cavity two 27 are in communication with the outside, the outlets of the pathogenic bacteria liquid adding channel 33 and the magnetic bead adding channel 25 are both located above the standard measuring dish 26, and a ring-shaped material groove 28 located on the outer side of the equipment cabinet main body 1 is arranged around the lower part of the placing cavity two 27.

[0057] The lower part of the placing cavity two 27 is provided with an observation cavity 29, the inside of the observation cavity 29 is fixedly provided with a microscope 30 for upward observation, the lower part of the microscope 30 is fixedly provided with a camera 31 for upward shooting, and the camera 31 is connected with a display screen 32 fixed on the outer side of the equipment cabinet main body 1.

[0058] The standard measuring dish 26 is made of completely visualized material, such as glass material.

[0059] When the detection and observation mechanism works, the microscope 30 is used for observing the condition that the magnetic beads adsorb pathogenic bacteria bodies in the standard measuring dish 26 in real time, then the camera 31 is used for shooting the image picture under the observation of the microscope 30 in real time, and the display screen 32 is used for displaying the image picture in real time, and since the amount of magnetic beads added at a time is small, if it is observed that the pathogenic bacteria colonies have not been formed after the magnetic beads adsorb pathogenic bacteria bodies, the magnetic beads are added until the pathogenic bacteria colonies are formed.

[0060] The working principle of the present application is as follows:

[0061] In application, the pathogenic bacteria liquid is centrifuged by the pathogenic bacteria liquid centrifugal adding mechanism, and the centrifuged pathogenic bacteria liquid is added on the detection observation mechanism. The magnetic beads are added one by one by the intermittent magnetic bead adding mechanism until the pathogenic bacteria bodies are observed to be adsorbed by the magnetic beads to form pathogenic bacteria colonies through the detection observation mechanism, so as to facilitate subsequent detection.

[0062] When the pathogenic bacteria liquid centrifugal adding mechanism works, the centrifugal rotating seat 5 is rotated by starting the driving motor 6, so as to realize the rotation of the pathogenic bacteria liquid centrifugal box 2. Under the action of the centrifugal force, the one-way switch valve cover 9 is opened, and the centrifuged pathogenic bacteria liquid enters the annular centrifugal cavity 3 from the centrifugal port 8, and then is added to the standard measuring dish 26 through the pathogenic bacteria liquid adding channel 33. If the pathogenic bacteria liquid in the standard measuring dish 26 overflows, it will flow into the annular material groove 28 for storage.

[0063] When the intermittent magnetic bead adding mechanism works, the trigger wheel 22 is rotated by starting the driving motor 23, and under the action of the trigger tooth head 24, the trigger frame 21 is abutted and moved upward. At this time, the intermittent magnetic bead adding box 13 is opened again by the trigger frame 21 abutting the intermittent switch valve core 16, and the magnetic beads in the intermittent magnetic bead adding box 13 enter the intermittent trigger cavity 20 through the adding port 15, and then are added to the standard measuring dish 26 through the magnetic bead adding channel 25. At this time, the magnetic beads begin to adsorb the pathogenic bacteria bodies and continuously approach.

[0064] When the detection observation mechanism works, the microscope 30 is used to observe the situation of the magnetic beads adsorbing the pathogenic bacteria bodies in the standard measuring dish 26 in real time, and then the camera 31 is used to shoot the image picture observed by the microscope 30 in real time, and the display screen 32 is used to display the image picture in real time. Since the amount of the magnetic beads added at a time is small, if the magnetic beads adsorbing the pathogenic bacteria bodies have not formed pathogenic bacteria colonies, the magnetic beads are added until the pathogenic bacteria colonies are formed.

[0065] It is obvious for those skilled in the art that the application is not limited to the details of the above exemplary embodiments, and the application can be implemented in other specific forms without departing from the spirit or essential characteristics of the application. Therefore, the embodiments should be regarded as exemplary and non-limiting, and the scope of the application is defined by the appended claims rather than the above description, and all changes falling within the meaning and scope of the equivalent elements of the claims are intended to be included in the application. Any reference signs in the claims should not be regarded as limiting the involved claims.

Claims

1. A pathogenic bacteria detection magnetic bead quantitative addition device, the magnetic bead quantitative addition device comprising a device cabinet main body (1), characterized in that: The device cabinet body (1) is sequentially provided with pathogenic bacteria liquid centrifugal adding mechanism, intermittent magnetic bead adding mechanism and detection observation mechanism from top to bottom, and the pathogenic bacteria liquid centrifugal adding mechanism and the intermittent magnetic bead adding mechanism are communicated with the detection observation mechanism respectively, and the condition of the magnetic bead adsorbing the pathogenic bacteria liquid can be directly observed through the detection observation mechanism; The intermittent magnetic bead adding mechanism comprises an intermittent magnetic bead adding box (13) which is detachably placed in a placing cavity one (14) formed in the middle of the device cabinet body (1), the front and back of the placing cavity one (14) are communicated with the outside, and the bottom of the intermittent magnetic bead adding box (13) is provided with an adding port (15) on both sides, the adding port (15) is provided with an intermittent switch assembly, and the intermittent switch assembly is located in the intermittent magnetic bead adding box (13); The adding port (15) is communicated with an intermittent trigger cavity (20) below, the intermittent trigger cavity (20) is internally provided with an intermittent trigger assembly, and the intermittent trigger cavity (20) is communicated with a magnetic bead adding channel (25) below. The intermittent switch assembly comprises an intermittent switch valve core (16), the intermittent switch valve core (16) is slidably assembled on a guide shaft two (17), the end of the guide shaft two (17) is fixedly provided with a spring baffle two (18), and the guide shaft two (17) is further sleeved with a spring two (19); The intermittent trigger assembly comprises a trigger frame (21), the trigger frame (21) is movably assembled in the intermittent trigger cavity (20), the upper end of the trigger frame (21) corresponds to the upper and lower positions of the two intermittent switch valve cores (16) respectively, the lower end of the trigger frame (21) is movably provided with a trigger wheel (22), the lower end of the trigger frame (21) is always in contact with the surface of the trigger wheel (22), and the trigger wheel (22) is in transmission connection with the rotating shaft of a driving motor (23) fixedly arranged outside the device cabinet body (1); Only one trigger tooth head (24) is arranged on the trigger wheel (22), and the surface of the trigger tooth head (24) and the lower end surface of the trigger frame (21) are both arc surfaces.

2. The pathogenic bacteria detection magnetic bead quantitative adding device according to claim 1, characterized in that: The pathogenic bacteria liquid centrifugal adding mechanism comprises a pathogenic bacteria liquid centrifugal box (2), the pathogenic bacteria liquid centrifugal box (2) is detachably placed in a ring-shaped centrifugal cavity (3) formed above the device cabinet body (1), a sealing cover (4) is threadedly assembled on the upper side of the ring-shaped centrifugal cavity (3), a centrifugal rotating seat (5) is movably assembled on the lower side of the ring-shaped centrifugal cavity (3), and the centrifugal rotating seat (5) is in transmission connection with the rotating shaft of a driving motor (6) below; Both sides of the pathogenic bacteria liquid centrifugal box (2) are respectively provided with a centrifugal port (8), and a one-way switch assembly is arranged at the centrifugal port (8) and located outside the pathogenic bacteria liquid centrifugal box (2); The lower sides of the ring-shaped centrifugal cavity (3) are respectively communicated with pathogenic bacteria liquid adding channels (33). 3.The pathogenic bacteria detection magnetic bead quantitative adding device according to claim 2, characterized in that: The detection observation mechanism comprises a standard measuring dish (26) which is detachably placed in a placing cavity two (27) opened at the lower part of the equipment cabinet body (1), the front and back of the placing cavity two (27) are communicated with the outside, the outlets of the path for adding pathogenic bacteria liquid (33) and the path for adding magnetic beads (25) are both correspondingly located above the standard measuring dish (26), and a ring-shaped material groove (28) is arranged outside the equipment cabinet body (1) around the lower part of the placing cavity two (27). A observation cavity (29) is opened below the placing cavity two (27), a microscope (30) for upward observation is fixed inside the observation cavity (29), a camera (31) for upward shooting is fixed below the microscope (30), and the camera (31) is connected with a display screen (32) fixed outside the equipment cabinet body (1).

4. The magnetic bead quantitative adding device for pathogenic bacteria detection according to claim 2, characterized in that: Two limiting heads (7) are fixed on the upper surface of the centrifugal rotating seat (5), and the centrifugal rotating seat (5) is limitedly assembled with the pathogenic bacteria liquid centrifugal box (2) through the limiting heads (7).

5. The magnetic bead quantitative adding device for pathogenic bacteria detection according to claim 2, characterized in that: The one-way switch assembly comprises a one-way switch valve cover (9) which is correspondingly arranged at the position of the centrifugal port (8), the two sides of the one-way switch valve cover (9) are respectively slidably assembled on a guide shaft one (10), the end of the guide shaft one (10) is fixed with a spring stopper one (11), and the guide shaft one (10) is further sleeved with a spring one (12).

6. The pathogenic bacteria detection magnetic bead quantitative adding device according to claim 1, characterized in that: The upper surface of the intermittent switch valve core (16) is an inclined surface.

7. The pathogenic bacteria detection magnetic bead quantitative adding device according to claim 3, characterized in that: The standard measuring dish (26) is made of completely visualized material.

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